LOCUS       DAZAOG010000001        81776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000001 DAZAOG010000000
VERSION     DAZAOG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.001"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..535
                     /locus_tag="ACHDLD_000001"
     CDS             17..535
                     /locus_tag="ACHDLD_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3361771.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="ACHDLD_000002"
     CDS             663..1346
                     /locus_tag="ACHDLD_000002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN3361772.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="ACHDLD_000003"
     tRNA            1655..1728
                     /locus_tag="ACHDLD_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="ACHDLD_000004"
     CDS             1887..2246
                     /locus_tag="ACHDLD_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN3361773.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="ACHDLD_000005"
     CDS             2369..4000
                     /locus_tag="ACHDLD_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN3361774.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="ACHDLD_000006"
     CDS             complement(4062..4703)
                     /locus_tag="ACHDLD_000006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN3361775.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4889..4965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="ACHDLD_000007"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="ACHDLD_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN3361776.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="ACHDLD_000008"
     CDS             5320..5664
                     /locus_tag="ACHDLD_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN3361777.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="ACHDLD_000009"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="ACHDLD_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN3361778.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6404..9211
                     /locus_tag="ACHDLD_000010"
     CDS             6404..9211
                     /locus_tag="ACHDLD_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3361779.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="ACHDLD_000011"
     CDS             9402..9893
                     /locus_tag="ACHDLD_000011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN3361780.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /locus_tag="ACHDLD_000012"
     CDS             9996..10985
                     /locus_tag="ACHDLD_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN3361781.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="ACHDLD_000013"
     CDS             11018..11821
                     /locus_tag="ACHDLD_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN3361782.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="ACHDLD_000014"
     CDS             11842..12756
                     /locus_tag="ACHDLD_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3361783.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="ACHDLD_000015"
     CDS             12839..13213
                     /locus_tag="ACHDLD_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN3361784.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="ACHDLD_000016"
     CDS             13352..13597
                     /locus_tag="ACHDLD_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3361785.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13769..14833
                     /locus_tag="ACHDLD_000017"
     CDS             13769..14833
                     /locus_tag="ACHDLD_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN3361786.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15079..15504
                     /locus_tag="ACHDLD_000018"
     CDS             15079..15504
                     /locus_tag="ACHDLD_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3361787.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15497..15946
                     /gene="nusB"
                     /locus_tag="ACHDLD_000019"
     CDS             15497..15946
                     /gene="nusB"
                     /locus_tag="ACHDLD_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN3361788.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16043..16897
                     /locus_tag="ACHDLD_000020"
     CDS             16043..16897
                     /locus_tag="ACHDLD_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN3361789.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16898..18235
                     /gene="xseA"
                     /locus_tag="ACHDLD_000021"
     CDS             16898..18235
                     /gene="xseA"
                     /locus_tag="ACHDLD_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN3361790.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18240..18464
                     /locus_tag="ACHDLD_000022"
     CDS             18240..18464
                     /locus_tag="ACHDLD_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN3361791.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18466..19356
                     /locus_tag="ACHDLD_000023"
     CDS             18466..19356
                     /locus_tag="ACHDLD_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN3361792.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19365..20183
                     /locus_tag="ACHDLD_000024"
     CDS             19365..20183
                     /locus_tag="ACHDLD_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN3361793.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20290..20739
                     /locus_tag="ACHDLD_000025"
     CDS             20290..20739
                     /locus_tag="ACHDLD_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3361794.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20757..22439
                     /gene="recN"
                     /locus_tag="ACHDLD_000026"
     CDS             20757..22439
                     /gene="recN"
                     /locus_tag="ACHDLD_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN3361795.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22494..22610)
                     /locus_tag="ACHDLD_000027"
     CDS             complement(22494..22610)
                     /locus_tag="ACHDLD_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN3361796.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22683..23639)
                     /locus_tag="ACHDLD_000028"
     CDS             complement(22683..23639)
                     /locus_tag="ACHDLD_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3361797.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23810..24178
                     /locus_tag="ACHDLD_000029"
     CDS             23810..24178
                     /locus_tag="ACHDLD_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN3361798.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24348..24779
                     /gene="mraZ"
                     /locus_tag="ACHDLD_000030"
     CDS             24348..24779
                     /gene="mraZ"
                     /locus_tag="ACHDLD_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN3361799.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24800..25759
                     /gene="rsmH"
                     /locus_tag="ACHDLD_000031"
     CDS             24800..25759
                     /gene="rsmH"
                     /locus_tag="ACHDLD_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN3361800.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25764..26165
                     /gene="ftsL"
                     /locus_tag="ACHDLD_000032"
     CDS             25764..26165
                     /gene="ftsL"
                     /locus_tag="ACHDLD_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN3361801.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26166..28358
                     /locus_tag="ACHDLD_000033"
     CDS             26166..28358
                     /locus_tag="ACHDLD_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN3361802.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28384..29346
                     /gene="mraY"
                     /locus_tag="ACHDLD_000034"
     CDS             28384..29346
                     /gene="mraY"
                     /locus_tag="ACHDLD_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN3361803.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29360..30721
                     /gene="murD"
                     /locus_tag="ACHDLD_000035"
     CDS             29360..30721
                     /gene="murD"
                     /locus_tag="ACHDLD_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN3361804.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30747..31835
                     /gene="murG"
                     /locus_tag="ACHDLD_000036"
     CDS             30747..31835
                     /gene="murG"
                     /locus_tag="ACHDLD_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN3361805.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31850..33070
                     /locus_tag="ACHDLD_000037"
     CDS             31850..33070
                     /locus_tag="ACHDLD_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN3361806.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33226..34551
                     /gene="ftsA"
                     /locus_tag="ACHDLD_000038"
     CDS             33226..34551
                     /gene="ftsA"
                     /locus_tag="ACHDLD_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN3361807.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34574..35815
                     /gene="ftsZ"
                     /locus_tag="ACHDLD_000039"
     CDS             34574..35815
                     /gene="ftsZ"
                     /locus_tag="ACHDLD_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN3361808.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35832..36509
                     /locus_tag="ACHDLD_000040"
     CDS             35832..36509
                     /locus_tag="ACHDLD_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN3361809.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36523..37122
                     /locus_tag="ACHDLD_000041"
     CDS             36523..37122
                     /locus_tag="ACHDLD_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN3361810.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37203..37424
                     /locus_tag="ACHDLD_000042"
     CDS             37203..37424
                     /locus_tag="ACHDLD_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN3361811.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37473..38255
                     /locus_tag="ACHDLD_000043"
     CDS             37473..38255
                     /locus_tag="ACHDLD_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN3361812.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38276..38983
                     /locus_tag="ACHDLD_000044"
     CDS             38276..38983
                     /locus_tag="ACHDLD_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN3361813.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39245..42043
                     /gene="ileS"
                     /locus_tag="ACHDLD_000045"
     CDS             39245..42043
                     /gene="ileS"
                     /locus_tag="ACHDLD_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN3361814.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42092..43618)
                     /gene="zwf"
                     /locus_tag="ACHDLD_000046"
     CDS             complement(42092..43618)
                     /gene="zwf"
                     /locus_tag="ACHDLD_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN3361815.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43633..44280)
                     /locus_tag="ACHDLD_000047"
     CDS             complement(43633..44280)
                     /locus_tag="ACHDLD_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN3361816.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44464..44793)
                     /locus_tag="ACHDLD_000048"
     CDS             complement(44464..44793)
                     /locus_tag="ACHDLD_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN3361817.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44970..45698
                     /gene="sfsA"
                     /locus_tag="ACHDLD_000049"
     CDS             44970..45698
                     /gene="sfsA"
                     /locus_tag="ACHDLD_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN3361818.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45714..46727
                     /locus_tag="ACHDLD_000050"
     CDS             45714..46727
                     /locus_tag="ACHDLD_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3361819.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46727..48004
                     /locus_tag="ACHDLD_000051"
     CDS             46727..48004
                     /locus_tag="ACHDLD_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3361820.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48067..50769
                     /locus_tag="ACHDLD_000052"
     CDS             48067..50769
                     /locus_tag="ACHDLD_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3361821.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50921..51238
                     /locus_tag="ACHDLD_000053"
     CDS             50921..51238
                     /locus_tag="ACHDLD_000053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3361822.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51268..51588
                     /locus_tag="ACHDLD_000054"
     CDS             51268..51588
                     /locus_tag="ACHDLD_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3361823.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51696..53156
                     /locus_tag="ACHDLD_000055"
     CDS             51696..53156
                     /locus_tag="ACHDLD_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3361824.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53224..53445
                     /locus_tag="ACHDLD_000056"
     CDS             53224..53445
                     /locus_tag="ACHDLD_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361825.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53476..53657
                     /locus_tag="ACHDLD_000057"
                     /pseudo
     CDS             53476..53657
                     /locus_tag="ACHDLD_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
     gene            53657..54070
                     /locus_tag="ACHDLD_000058"
     CDS             53657..54070
                     /locus_tag="ACHDLD_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN3361826.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54193..55374
                     /locus_tag="ACHDLD_000059"
     CDS             54193..55374
                     /locus_tag="ACHDLD_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3361827.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55445..55609)
                     /locus_tag="ACHDLD_000060"
     CDS             complement(55445..55609)
                     /locus_tag="ACHDLD_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361828.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55763..55836
                     /locus_tag="ACHDLD_000061"
     tRNA            55763..55836
                     /locus_tag="ACHDLD_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55797..55799,aa:Arg,seq:cct)
     gene            complement(55882..55995)
                     /locus_tag="ACHDLD_000062"
                     /pseudo
     CDS             complement(55882..55995)
                     /locus_tag="ACHDLD_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(56378..57610)
                     /locus_tag="ACHDLD_000063"
     CDS             complement(56378..57610)
                     /locus_tag="ACHDLD_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN3361829.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57864..58433
                     /locus_tag="ACHDLD_000064"
     CDS             57864..58433
                     /locus_tag="ACHDLD_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3361830.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58611..59051)
                     /locus_tag="ACHDLD_000065"
     CDS             complement(58611..59051)
                     /locus_tag="ACHDLD_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN3361831.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59209..59973
                     /gene="map"
                     /locus_tag="ACHDLD_000066"
     CDS             59209..59973
                     /gene="map"
                     /locus_tag="ACHDLD_000066"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN3361832.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60005..60928
                     /locus_tag="ACHDLD_000067"
     CDS             60005..60928
                     /locus_tag="ACHDLD_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN3361833.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61004..62143
                     /locus_tag="ACHDLD_000068"
     CDS             61004..62143
                     /locus_tag="ACHDLD_000068"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN3361834.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62136..62936
                     /locus_tag="ACHDLD_000069"
     CDS             62136..62936
                     /locus_tag="ACHDLD_000069"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3361835.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62936..63763
                     /locus_tag="ACHDLD_000070"
     CDS             62936..63763
                     /locus_tag="ACHDLD_000070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3361836.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63768..64475
                     /locus_tag="ACHDLD_000071"
     CDS             63768..64475
                     /locus_tag="ACHDLD_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3361837.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            64575..65441
                     /gene="rfbA"
                     /locus_tag="ACHDLD_000072"
     CDS             64575..65441
                     /gene="rfbA"
                     /locus_tag="ACHDLD_000072"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN3361838.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65455..66027
                     /gene="rfbC"
                     /locus_tag="ACHDLD_000073"
     CDS             65455..66027
                     /gene="rfbC"
                     /locus_tag="ACHDLD_000073"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN3361839.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66049..67077
                     /gene="rfbB"
                     /locus_tag="ACHDLD_000074"
     CDS             66049..67077
                     /gene="rfbB"
                     /locus_tag="ACHDLD_000074"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN3361840.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67175..68026
                     /gene="rfbD"
                     /locus_tag="ACHDLD_000075"
     CDS             67175..68026
                     /gene="rfbD"
                     /locus_tag="ACHDLD_000075"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN3361841.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68061..70094
                     /locus_tag="ACHDLD_000076"
     CDS             68061..70094
                     /locus_tag="ACHDLD_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361842.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70138..71418
                     /locus_tag="ACHDLD_000077"
     CDS             70138..71418
                     /locus_tag="ACHDLD_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN3361843.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71628..72434
                     /locus_tag="ACHDLD_000078"
     CDS             71628..72434
                     /locus_tag="ACHDLD_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3361844.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72446..73666
                     /locus_tag="ACHDLD_000079"
     CDS             72446..73666
                     /locus_tag="ACHDLD_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3361845.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73656..75242
                     /locus_tag="ACHDLD_000080"
     CDS             73656..75242
                     /locus_tag="ACHDLD_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3361846.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75281..77419
                     /locus_tag="ACHDLD_000081"
     CDS             75281..77419
                     /locus_tag="ACHDLD_000081"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3361847.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77787..78770)
                     /locus_tag="ACHDLD_000082"
     CDS             complement(77787..78770)
                     /locus_tag="ACHDLD_000082"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3361848.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79148..80539
                     /locus_tag="ACHDLD_000083"
     CDS             79148..80539
                     /locus_tag="ACHDLD_000083"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN3361849.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80555..81595
                     /locus_tag="ACHDLD_000084"
     CDS             80555..81595
                     /locus_tag="ACHDLD_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3361850.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81614..>81776
                     /locus_tag="ACHDLD_000085"
     CDS             81614..>81776
                     /locus_tag="ACHDLD_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN3361851.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIER"
BASE COUNT        26680 a        13825 c        17337 g        23934 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13681 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13741 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13801 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    13861 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    13921 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    13981 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14041 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14101 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14161 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14221 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14281 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14341 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14401 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14461 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14521 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14581 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14641 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14701 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14761 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    14821 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    14881 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    14941 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15001 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15061 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15121 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15181 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15241 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15301 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15361 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15421 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15481 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15541 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15601 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15661 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15721 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15781 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    15841 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    15901 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    15961 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16021 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16081 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16141 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16201 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16261 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16321 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16381 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16441 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16501 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16561 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16621 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16681 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16741 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16801 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    16861 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    16921 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    16981 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17041 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17101 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17161 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17221 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17281 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17341 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17401 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17461 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17521 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17581 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17641 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17701 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17761 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    17821 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    17881 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    17941 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18001 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18061 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18121 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18181 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18241 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18301 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18361 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18421 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18481 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18541 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18601 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18661 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18721 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18781 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    18841 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    18901 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    18961 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19021 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19081 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19141 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19201 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19261 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19321 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19381 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19441 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19501 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19561 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19621 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19681 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19741 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19801 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    19861 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    19921 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    19981 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20041 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20101 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20161 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20221 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20281 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20341 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20401 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20461 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20521 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20581 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20641 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20701 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20761 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    20821 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    20881 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    20941 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21001 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21061 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21121 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21181 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21241 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21301 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21361 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21421 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21481 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21541 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21601 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21661 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21721 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21781 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    21841 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    21901 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    21961 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22021 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22081 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22141 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22201 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22261 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22321 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22381 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22441 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22501 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22561 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22621 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22681 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22741 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22801 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    22861 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    22921 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    22981 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23041 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23101 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23161 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23221 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23281 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23341 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23401 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23461 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23521 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23581 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23641 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23701 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23761 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    23821 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    23881 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    23941 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24001 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24061 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24121 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24181 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24241 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24301 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24361 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24421 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24481 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24541 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24601 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24661 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24721 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24781 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    24841 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    24901 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    24961 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25021 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25081 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25141 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25201 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25261 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25321 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25381 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25441 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25501 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25561 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25621 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25681 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25741 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25801 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    25861 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    25921 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    25981 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26041 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26101 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    26161 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26221 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26281 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26341 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26401 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    26461 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26521 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26581 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26641 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26701 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26761 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    26821 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    26881 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    26941 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27001 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27061 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27121 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27181 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27241 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    27301 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27361 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27421 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27481 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27541 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27601 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27661 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27721 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    27781 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    27841 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    27901 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    27961 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28021 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28081 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28141 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28201 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28261 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28321 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28381 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28441 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28501 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28561 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28621 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28681 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28741 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28801 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    28861 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    28921 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    28981 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29041 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29101 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29161 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29221 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29281 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29341 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29401 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29461 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29521 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29581 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29641 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29701 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29761 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    29821 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    29881 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    29941 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30001 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30061 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30121 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30181 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30241 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30301 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30361 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30421 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30481 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30541 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30601 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30661 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30721 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30781 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    30841 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    30901 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    30961 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31021 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31081 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31141 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31201 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31261 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31321 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31381 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31441 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31501 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31561 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31621 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31681 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31741 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31801 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    31861 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    31921 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    31981 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32041 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32101 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32161 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32221 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32281 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32341 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32401 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32461 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32521 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32581 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32641 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32701 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32761 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    32821 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    32881 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    32941 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33001 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33061 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33121 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33181 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33241 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33301 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33361 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33421 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33481 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33541 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33601 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33661 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33721 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33781 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    33841 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    33901 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    33961 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34021 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34081 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34141 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34201 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34261 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34321 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34381 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34441 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34501 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34561 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34621 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34681 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34741 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34801 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    34861 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    34921 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    34981 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35041 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35101 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35161 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35221 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35281 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35341 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35401 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35461 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35521 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35581 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35641 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35701 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35761 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    35821 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    35881 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    35941 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36001 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36061 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36121 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36181 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36241 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36301 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36361 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36421 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36481 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36541 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36601 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36661 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36721 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36781 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    36841 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    36901 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    36961 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37021 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37081 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37141 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37201 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37261 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37321 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37381 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37441 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37501 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37561 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37621 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37681 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37741 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37801 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    37861 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    37921 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    37981 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38041 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38101 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38161 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38221 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38281 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38341 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38401 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38461 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38521 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38581 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38641 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38701 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38761 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    38821 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    38881 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    38941 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39001 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39061 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39121 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39181 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39241 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39301 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39361 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39421 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39481 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39541 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39601 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39661 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39721 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39781 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    39841 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    39901 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    39961 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40021 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40081 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40141 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40201 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40261 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40321 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40381 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40441 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40501 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40561 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40621 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40681 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40741 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40801 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    40861 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    40921 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    40981 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41041 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41101 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41161 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41221 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41281 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41341 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41401 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41461 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41521 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41581 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41641 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41701 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41761 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    41821 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    41881 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    41941 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42001 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42061 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42121 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42181 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42241 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42301 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42361 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42421 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42481 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42541 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42601 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42661 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42721 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42781 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    42841 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    42901 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    42961 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43021 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43081 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43141 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43201 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43261 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43321 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43381 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43441 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43501 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43561 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43621 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43681 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43741 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43801 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    43861 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    43921 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    43981 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44041 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44101 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44161 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44221 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44281 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44341 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44401 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44461 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44521 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44581 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44641 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44701 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44761 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    44821 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    44881 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    44941 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45001 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45061 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45121 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45181 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45241 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45301 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45361 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45421 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45481 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45541 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45601 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45661 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45721 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45781 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    45841 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    45901 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    45961 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46021 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46081 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46141 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46201 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46261 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46321 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46381 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46441 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46501 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46561 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46621 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46681 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46741 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46801 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    46861 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    46921 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    46981 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47041 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47101 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47161 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47221 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47281 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47341 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47401 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47461 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47521 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47581 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47641 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47701 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47761 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    47821 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    47881 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    47941 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48001 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48061 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48121 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48181 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48241 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48301 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48361 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48421 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48481 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    48541 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48601 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48661 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48721 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48781 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    48841 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    48901 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    48961 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49021 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49081 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49141 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49201 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49261 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49321 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49381 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49441 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49501 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49561 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49621 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49681 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49741 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49801 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    49861 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    49921 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    49981 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50041 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50101 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50161 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50221 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50281 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50341 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50401 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50461 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50521 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50581 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50641 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50701 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50761 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    50821 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    50881 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    50941 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51001 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51061 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51121 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51181 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51241 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51301 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51361 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51421 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51481 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51541 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51601 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51661 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51721 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51781 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    51841 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    51901 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    51961 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52021 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52081 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52141 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52201 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52261 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52321 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52381 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52441 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    52501 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52561 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52621 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52681 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52741 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52801 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    52861 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    52921 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    52981 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53041 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53101 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53161 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53221 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53281 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53341 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53401 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53461 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53521 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53581 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaga gaagaagcaa
    53641 aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta
    53701 cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac
    53761 tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg
    53821 cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa
    53881 gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca
    53941 ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg
    54001 aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc
    54061 aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga
    54121 aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga
    54181 aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg
    54241 tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt
    54301 cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca
    54361 agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc
    54421 ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc
    54481 atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca
    54541 ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat
    54601 caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg
    54661 gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa
    54721 cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat
    54781 taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag
    54841 cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc
    54901 agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc
    54961 ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc
    55021 acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt
    55081 aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat
    55141 gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa
    55201 acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat
    55261 cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa
    55321 acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa
    55381 acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa
    55441 aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag
    55501 ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag
    55561 atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc
    55621 tccctacatt atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt
    55681 ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag
    55741 ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta
    55801 agcgataggt cgctggttca attccagtca ggaacgtaca aataacccct gtatcctttg
    55861 cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc tatgtcctaa
    55921 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgcgtatg
    55981 tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta ctaaagaaaa
    56041 acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa ttaaacgatt
    56101 tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac tctagcggta
    56161 taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt ttattatttt
    56221 tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca tataaaaaaa
    56281 cggtggggtt gtttagcgaa ataaaggatc taagccaata gcttagatcc tttattctat
    56341 gctttatatt attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga
    56401 aaatcggtac tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta
    56461 ccatgaaatc tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg
    56521 acatttctgt accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg
    56581 atgcgttttc atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag
    56641 ggtcaggtac aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt
    56701 ttgctaagac atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag
    56761 taaatttcat acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt
    56821 aaccatctac tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca
    56881 tgaatttttc tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca
    56941 atccgataat gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat
    57001 tcaattcttc tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt
    57061 cttcttcata gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt
    57121 cttctggcag ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag
    57181 tccaactgac tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg
    57241 ctgattgata agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag
    57301 aaacaacgct gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag
    57361 attgtggtac attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct
    57421 ggtcgtcagt ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga
    57481 tcaatcgagc aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt
    57541 ctgtggctac gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat
    57601 cagataacat aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc
    57661 atttaattat gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg
    57721 ttaagtttca tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact
    57781 aattttgcat atcaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa
    57841 aaaaattaag gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt
    57901 tggcagctgc ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca
    57961 tcgcgaacaa aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg
    58021 acttgagaca agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg
    58081 ttgagcatac agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa
    58141 gagagaagaa gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc
    58201 agaatttctc ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc
    58261 tttcagaaga gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg
    58321 cagctttgat gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc
    58381 aagacagtat agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact
    58441 caaaagttcc acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt
    58501 ttcttacagc gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa
    58561 agagccatac agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt
    58621 gcgactagtt ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga
    58681 tccactttaa cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa
    58741 tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa
    58801 atttttccca atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc
    58861 agatcgccat catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca
    58921 tctgcatcta cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct
    58981 tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt
    59041 gctaaagcca tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa
    59101 acatcattga aaagaaaata aaacctaaag acacctgggg aatgtcattt ggctaaatgt
    59161 atgccgctat gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta
    59221 aaatcagcac gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat
    59281 aaaaatctgc gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc
    59341 agaaactata tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa
    59401 tatgcgactt gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct
    59461 ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct
    59521 gattcttgtt gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa
    59581 gtaacgaaga aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt
    59641 gacatcggtc atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat
    59701 tttattggtc atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga
    59761 gaagctggaa aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt
    59821 aataccggaa cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac
    59881 ggcggacttt cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt
    59941 ttgacatcac aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag
    60001 gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac
    60061 gactcaaaag cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta
    60121 tttactcttg tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca
    60181 catcgatcaa gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca
    60241 gtttatcggt cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt
    60301 ttctgcatta gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat
    60361 gaataaagcg tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact
    60421 aattgtcatt gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg
    60481 acagctgatt ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca
    60541 gttccaaact ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat
    60601 ttatttaatt gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt
    60661 cgctactgtc ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc
    60721 aacgaggatc agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa
    60781 ttttgctgct acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt
    60841 gacgggtcat caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa
    60901 aactaaattt tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag
    60961 taatttataa taattatgac tcttaattaa agtaggtgcc catatgcttg tttcttttag
    61021 tatggttata agattttttc tgacaatttt attatcactg atattaacac ctgtattcaa
    61081 aatcatttca gtacaaacag gtatggtcga caaaccaaat gaaagaagaa taaataaagt
    61141 tccaatgcct tcagcgggcg gactgcctat ctttatttca tttgttatat cgactttatt
    61201 cttattccgt aaaattatcc ctcaatcgta tattgtaccg atcttgattg cttctttagt
    61261 aatcatagtc acaggagtgc tcgatgataa atatgagctg acacctaaac aaaaaagtat
    61321 agggatcttg attgcttctc ttattattta ttttgtagca gatatacgta ttgattcctt
    61381 cacgcttcct tttattggat atatccaact aggctggctt agttttcccg taacgatttt
    61441 ttggatcttc ggtatcacga atgcggtcaa tctgattgac ggtttggatg ggctggcggc
    61501 agggatttca ttgatcggtt taattacgat aggaatcatt ggatattttt tcctccatgc
    61561 ttctacagtg tatattccag ttgttatttt ctgtcttgta gcaagtatta tcggtttttt
    61621 tccttacaat ttttatccgg caaagattta tttaggcgat acaggagctc tattcctagg
    61681 gtttatgatg gccgtccttt cactacaagg gctgaagaac gtgacttttg tttcatcgat
    61741 ttctttgctg atcgtcatgg gagtgccggt aacagacacg ttttttgcta tcattaggag
    61801 aaaagcaaac cgtgtttctt tttctacagc agataaaaaa catcttcatc accgactaat
    61861 cagtttaggc tttactcata aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt
    61921 ttctttcaca gctatggtca tgaattatac tggaagaatc ggaacgatcg tattgattat
    61981 tgccatgttg tttgctgcaa ttttattatt tgaattgatt ggtctgatca atgagaaaca
    62041 tcagtttatt ttaaaaacat tgcgattttt agggaataaa gagtaccgta cgcaagtaaa
    62101 acagaaatat ttgaagaaat ggaaccgtca tatagatgaa taacaaatta aaccagccga
    62161 tccttattag tattgtgacg gcaaacagtg ataaaatttt tcggacacta gataatttta
    62221 tttcaactgt aaaagatgaa acagcagtaa aaatccgaat ttttgacaac aattcacttc
    62281 ccgagtacaa aaaaagacta acagagtata ggaaatatcc ctacatcgat atctattttc
    62341 atgatgaaaa ttccggattt ggttatggac ataatcacaa tctgatagct agcgattctc
    62401 gctatggcat tatttgtaac ccagatattt tagtggatga agagacagtt acagcattgg
    62461 tcttgttatt gaaagaacat ccggaatgtg cgatgttagc acctaagatt cttaatgaag
    62521 atggaacaac tcagcacctc atccgtgagc agctggctgt ttttgattac gttttgcgct
    62581 ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc
    62641 gaggattgcc tactgatgct aatagttatg tacggatgat ctcaggtagt tttatggtag
    62701 ctgatattga taaatttaaa gagataaagg ggtttgatga gcgtttcttc atgtactttg
    62761 aagacaacga cttgtgtttg agttttgaaa aagcaggtta caagttattg tacacaccct
    62821 tttatgcagt tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg
    62881 tgtttttgac ttctatgatg aaatttttca ataagtgggg gtggcgtttt ttctgatgaa
    62941 accagaaata ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat ctccagctta
    63001 tggaccatta agcaaagcac ttagcgaaaa gcaaattcag cttattattt acgataatag
    63061 tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat atcgtttatc atcatgatcc
    63121 gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg tctcaagcag atgaaggaac
    63181 acgttgtctc gtcactctgg atcaggatac gaggctagtc gacgattatt ttgaactatt
    63241 gcgtaaagta acctttacag atgaatgtgt tgctgcagtt ccgatgattt tttctgggag
    63301 ccgacaagtt tctcccctat attcagatcg ttatatcaat cgtacagctc aagcagtaga
    63361 agttgaaggc ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt
    63421 caaatttcta aaaaagattg gcgggtttaa tcctgctttt tctttagatt ttttggatca
    63481 ttggctgttt tggaaagtgg atcagttgga aaaaactgtg gagatccttc ctgtacgaat
    63541 ggaacatgat ctttcggttt tagactatga aaaagtttca agtaatcgat accgttctat
    63601 tttacaagca gaaaatcgat tttatcatga atatgatcaa aggcatttat ccagtcaccg
    63661 acgacaatta ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg
    63721 gcggatgact ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt
    63781 gtatagctac ctttaatgga ggcagatatt taagagagca gttagatagt attttatctc
    63841 agctttcagc agaagatgaa gtagtcattt ctgatgatgg gtcagtagat gatacatggg
    63901 atatattaac tgaatacgca gaaaaagata agcgaatcca gcttttttcg gggcctcgtc
    63961 aaggattgat cgctaatttt ggttatgcga tccagcagac gaaaggagat ctaatctttt
    64021 tggcagacca agatgatgtt tggttggaga caaaagtgaa aaagatcact gactattttg
    64081 agcagaatcc ggatcagcta gtagtagttt ctgatttaat cattgtggac agcgagctgc
    64141 aagagatcca tgattcgtat ttttcgtttc gaaattccaa agaaggatgg cttaataatg
    64201 tgataagaag tcactatatt ggtgctggta tggctttccg gtcttcttta aaacccgata
    64261 ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg gattggtttg ctgggaggaa
    64321 agaagactgg gttcctaaga gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga
    64381 gtgagattga aacacattct aaattacggc aaaaaatttc ttggcgtgtt catctgatta
    64441 cggcgttgat caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa tccttaatat
    64501 ttccatgaca aatataacaa ttgatgagat aataacaaag tgaaatttga aatcaaaaag
    64561 gagagcagat atccatgaag ggaatcattc ttgctggggg cagcggaaca cgtctgtacc
    64621 ctttaacaaa ggcaacatcg aaacaattga tgccgattta tgacaaacca atgatttatt
    64681 atccaatgtc tactctgatg ttggcgggaa tcaaagaaat tctgattatt tcaacgccac
    64741 aagatacacc acgtttcaag gaattatttg gaaacggaaa tgatttagga ctgcagatcg
    64801 aatatgcagt acaagaaagt ccagatggat tggcacaagc gtttatcatt ggagaagaat
    64861 ttattggaga tgatagtgtc tgcttggtat taggggacaa tatttactat ggtggcggtc
    64921 tgtctaaaat gttgcaacga gcagcttcaa aagatacagg cgcgacagta tttggctatc
    64981 atgtcaatga tccagaacgc tttggtgtag tagaatttga tgagaatatg caagctttgt
    65041 cgattgaaga aaagcctgct cagccaaaat caaattacgc tgtaactggc ctttattttt
    65101 atgacaatga ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt ggagaactag
    65161 agattacgga tgtcaataaa gcatatttgg ataaaggaaa actatccgta gaacttatgg
    65221 gacgcggatt tgcttggctt gatacaggaa cacatgaatc attgcttgaa gcatctacct
    65281 ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa gaaattgctt
    65341 accgtatggg ctacatcgat aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa
    65401 atcaatacgg tcaatattta ttgaatttgg ccgctgaata aggagtcctt acagatgaaa
    65461 gtaacagata caaaattaac agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat
    65521 cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg attgaaatat
    65581 gactttgttc aagacaacca ttcactatca gctgaagctg gtgtccttcg ggggcttcat
    65641 ttccaaaaag gagaagctgc acagacaaaa ttgattcggg tagtcactgg agcagtttta
    65701 gatgtagtag tggatatccg caaaggtagc ccgacttatg gtcaatggga cggatttatc
    65761 ttatctgaac ataatcatcg acagttgctt gtacctaaag gatatgccca tggattcgtt
    65821 actttgacac caaatgtgaa cttcctttat aagtgtgata attactataa tgcagaagca
    65881 gatgggggca tcgcatttga tgatccagat ttagcgattg attggccaat tgatccatca
    65941 agagcgatta tgtcggaaaa agatcaccgt catccgacat taaaagaatt tgaagcagaa
    66001 aatccatttg tttacggaga aatctaagaa ttaaggagaa attttcttat gaaaaacatc
    66061 attgttaccg gaggtgccgg attcatcggc tctaatttcg ttcattacgt ggtaaacaat
    66121 catccagaag tgcatgtcac tgttttggat aaactgactt atgcaggaaa caaagagaac
    66181 ttggccggac ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa
    66241 ttagtggacc gtctagtaaa agaagcggac gcagttgttc attacgcggc agaatcccat
    66301 aacgataatt cattaaaaga tcctttccca tttgtacaaa cgaatatcat tggtacgtat
    66361 acacttttag aagcttgccg caagtatgat gtacgctatc atcacgtatc tactgacgag
    66421 gtttacggcg atcttccatt acgtgaagac ctgccaggac atggggaagg cgaaggagaa
    66481 aaattcactg cggaaacacc atataatcct tcaagtccat attcttctac aaaagctggt
    66541 tcagatctgt tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac catttcaaac
    66601 tgttcaaaca actatggtcc ttatcagcat attgaaaaat ttattcctcg ccaagtaacg
    66661 agtatcttaa gcggtattcg tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg
    66721 atccatacga atgatcattc ttcagcagtg tggctgattt tgacgaaagg cagaattggc
    66781 gaaacctatc tgatcggtgc agacggtgaa gaagataata agaccgtgat ggaattgatc
    66841 ttagaaatga tgggccaacc aaaagacgca tacgaccatg taaacgaccg cgcagggcac
    66901 gatttgcgtt atgcaatcga ttcgacaaaa ttgcgagaag agctaggttg gcagcctgag
    66961 tttacgaact tccgtgatgg gttggctgac acgatcaaat ggtacgaaga acatgaagac
    67021 tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg acaataatac
    67081 agaggaaatt tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct
    67141 tttttaaaat aaaaatcaag gagcggataa ccatatgttt ttattaactg gaggaaatgg
    67201 acaattaggt acagaattgc gccatttatt agatgaaaaa aatctgccat atgtttcaac
    67261 agatgcaaaa gacttggata tcacagatgc tgagaaaaca atggcttata ttacagaatt
    67321 aaaacccgat gttattttcc attgcgcagc ttacacagca gtagataaag cagaagaaga
    67381 agcaaaagaa ttagatgaga aaatcaatgt cgatggcaca agaaatgtag cacttgcagc
    67441 aaaagctgtg aatgcacaat tcgtctatat cagtacagac tatgttttcg atggaaaaaa
    67501 caaaaatgta gaatacgaag tagacgacca aacgaatcca ctaaatgaat atggtcgtac
    67561 gaaattatta ggcgaaaaag cagtagaaga gattttagaa gactattata tcatacgaac
    67621 ttcttgggtt ttcgggatct atggacacaa tttcgtcttc acgatgcaac gcctagcgga
    67681 aacccgcgac aaactgactg tagtaaatga ccagtttggg cgaccaactt ggacacgtac
    67741 tttggcagaa tttatggtat atgtcatcga agaaaaagca ccatttggta tttatcattt
    67801 gtcgaacgaa aatagctgtt cttggtatga gttcgcaaaa gaaatattga aagatacaga
    67861 tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa aaagcaacac gtccgcagta
    67921 ttcagtcatg agcctgaaaa agacagaagc gttaggtttt gtcataccaa cttggcaaga
    67981 agcacttgcg caaatgctgg aagctgccaa aaaaatgaat aagtaatttc ctttttatca
    68041 aatagaattt ggagcatgat atggaaaaac aaacaagaag tgcacgacga aaagaaaagc
    68101 caaaagcgaa tcaggcagat tcgaatttgg tgcttgacct gtttctaaaa ctccgctatc
    68161 ttattggcgt tttagtcatc gtggtcatcg ttacttttaa tttaaacgga agttcacttg
    68221 gtgtttggga taaatatgtg tcgcaaagag acgatgggaa aaaaactgat gtgatttttg
    68281 gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca gacacctttt tacctctcac
    68341 aagctgaaga ggacttttcg ttggtgaacg aggattattc attaaacgga caaaacatga
    68401 tcattgcgta taattctcct gtaaaggata ttacggttat cggaaaacca ttcaactggg
    68461 gattcttttt cctcggaaga gatcgaggac tttcctttta ttgggcgatg aaattagtcg
    68521 tcatggtctt gcttggattc gaagtactga tgatattaac gaagaggaaa aaagctctat
    68581 cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg ttcatgcaac
    68641 atgtgggcga tttgattttc tttactttgg gactgatgat tgctttttat cattatttct
    68701 acaatcatga taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt attttcggta
    68761 ttgggtttat tttagtgatt tacccagccc atcaagtgat gctggcgtac ttgctgattt
    68821 tttattttgt tggcctgctt atttactata gcaagaaagt ttcatgggat ctgttggatg
    68881 tagtgttgat tggaggggca gttttattta ttggcggagt catggttcat ttctggctaa
    68941 cgtcaaaaga cgcattgtta gcttctttga ataccttata tccaggaaaa cgggtaagta
    69001 ctggaggaaa gtggacgatt ggcgaatttt tttctttcct tacaaattgg aaaattcctt
    69061 ttgaagatat caactattcg aataactctg aagtggccct gttctatcac ttttttccaa
    69121 ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa caaaagttac
    69181 tgggacgtat tttgatgatt ttttgtctgt tttctatctt ttggattacc gttgggttgc
    69241 caaaatggct agcagaaatc actctgctgt cttatgttcc tccattaaga gccatcctta
    69301 cttttagttt tgcggcttgt ttattatcga tctggtttat cgcttatatt tggagagaag
    69361 atgttattcc tatttggtat aaattcattt tgttggcagt aaatgcatgg tcttatttct
    69421 atgcgattac gcattcgaaa atggataatt atttttcgga ttttgaaacc atcgcagttg
    69481 ctgtgttggg aacagtgatt ttagcttttg cattatttgg ctggagacgc ttgttttacc
    69541 tcgcttttgt cgccttagtt atcgtatctg gctttttcgt caatccagtc gtagaaggta
    69601 caggagcgat ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat caacaagatc
    69661 ctggtaaagt ttggttgtct gaagatgaat tatataactt caccccaaca ttgggagtga
    69721 aatccttcaa tacagtacgc ttttatccag atatagctgc atggaagaaa atcgatccga
    69781 aagaagaata cgagaagtat tataatcggt atgctcatac aagagctttt atcgctaagg
    69841 aagatacaaa gttcatttta gatcgtccag atatgttttc ggtcacgttg aattttgaag
    69901 acgcagaaaa attagggatc gactatgtga tcagtaaacg accattaact gattacaacc
    69961 aattatatga agcgcagttc acgcagctat atggaccgga taaagacggt tatatgattt
    70021 ttaaagtaga atatccggat tatccagatg taccacaaac acaaactcag gaaaatcagc
    70081 tagtcgtaca gtaataggct agctgagaat aatcctgaaa tattagtaga aaggagagtg
    70141 aagataattc aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg tttcttgctt
    70201 ttgttgctga cgctagtctt ttttgtttgg acagcagggg taggttttcc agcaactttg
    70261 tttatatata acaatagttc attacttatc gcttttagtg tggctatttt gctcatttta
    70321 aatgtttata aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct tgcccttttc
    70381 attttctttt cttactttcg ggaattaaga aatgagtctt tatttggaga cttactgttt
    70441 cctttggtta ttttattttg tttagtaatt aaatggatcg agtggacaaa atgggatcgc
    70501 ggtatttatc tgattgtctt tacggtcttc ctcggaatca cgttatatcg tgtatttaca
    70561 gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct ttctgatatt
    70621 tggatcaata cgaatacaat cggaagttct ttgatgactc ttgctttatt gatcagtgga
    70681 tttgccagtt cttttgaacg atggtatgtt cgaatcatcg gtgggatagc agtcgttgca
    70741 gcctttttag gtatttgggt ctgccaatct agaggtgcat tagtcgcgtt gattgttttt
    70801 gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc atttattgcc
    70861 tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt ctctgaaaaa
    70921 gtcaatttgt ttaccgatcg agaagatatt tggcataaat tctatcagac gctaggagaa
    70981 aaatctgaac aagttttact aggaatgaaa acatttatct tccagcgagg aaaccaattt
    71041 ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat aggatttggg
    71101 attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc agatttatcc
    71161 aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc atttatggag
    71221 gatacattga cttcataccc atgggttccg attatttata tcctgttggc tatggccgtt
    71281 catcgatatg atgaaccaga agttgcggag gaattctatg ttatggaaga ggcatatgac
    71341 gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca ttcttccagg
    71401 atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac cgtcgcagcc
    71461 ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc
    71521 ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa aatatggtaa
    71581 aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg tttaaagatt
    71641 tgtatttatt tatgaaggac atttatcaga acagaaaatt attgctacag ttttcgatca
    71701 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
    71761 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
    71821 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
    71881 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
    71941 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
    72001 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
    72061 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
    72121 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
    72181 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
    72241 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
    72301 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
    72361 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
    72421 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
    72481 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
    72541 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
    72601 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
    72661 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
    72721 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
    72781 aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
    72841 tgaatttgct gacatcggag accatattta tcaacctgta aaaacttatt caagcgggat
    72901 gttcgttcgt ctagcttttg ctgtagcaat caatgtgaat cccgatattt tgattgtgga
    72961 tgaagcattg gcagtaggcg atcttgaatt tcaactaaaa tgtatggaaa aatttacaga
    73021 aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatatcagtt cgattcgtcg
    73081 tttttgtgat cgttcctatt ggttgaaaaa cggagaagtc gtcgaatatg gcgatacgat
    73141 ggaagtaacg acgaattatg aaaacttttt aaagaaaaaa tcagttaaaa cagtggatcg
    73201 gaataaaaac caagtagaac ggttcgccgc tccagatatc gtcgacgtga cgaaagcgga
    73261 attgttggat aaagacaaaa atccggtgga tatgctggaa caaggagaaa aactatacgt
    73321 taaagtaact tatgaagtaa aagatgatac aatcaaaaat ccggttttag gaattgcgct
    73381 acgaacagtc gacaattttt atgtatgcgg acttaatact ctactagatg gtatcaagat
    73441 tccttggaaa aaaggagaga atatctttta tctggagtat gaaaagttag gtttgttagc
    73501 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
    73561 taagacaaag tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
    73621 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
    73681 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
    73741 tagagtttgg tcccggaaac ggccgattaa cgaaacattt aatcggagca aaacaatgtg
    73801 aagtgagcat tgttgagtta gataaggaat tgttcgattt tgtcagcgaa tttgctcaag
    73861 atggatttta tggtgatatc gaaagttttg agtgggcaaa ctattatgct ggacagacat
    73921 ttgattacgt cttgtttgcg gatgttttag aacatttggt agatcctgga aaaacattga
    73981 agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc cctaacttgg
    74041 cgcataattc tgtcatgatc gatttgttca ataaccaatt gccgtgggct tcttatggtc
    74101 tcctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa gtttttgaaa
    74161 aagcgggatt attcattaat attgaagatt atttgtactt agctgtaggt gatacagaat
    74221 taaaatcaac ttatgaagaa ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat
    74281 ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagca caaagttcca
    74341 ttgcagagcc acaaaattct aattatgtaa aagtgttaga agtaacccag caaacgaaac
    74401 aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa
    74461 gtttccccgt atcagaaaca acagaaagaa tggtgtttcg cttcgcgcaa caacctagct
    74521 ttatcgaatt cagcgcagaa gctgccggag aaaaactaac atttatcgat tcgaatgctg
    74581 tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaattacca gagttcgttc
    74641 taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat
    74701 tgacaccaac aatgaaagaa ttgttggaaa cgattcgtcc catggctgaa gtcacaaagc
    74761 agctttctga gaaaaatgac gaattagaaa gagaaaatca tcatcttctt gaggaaaata
    74821 caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg
    74881 atgaatggac aataaaacat agactaggac gacgtaagaa agaaacttcg aaaaaaatcc
    74941 aagaaaaaga actttctata tgtattgacg agaaaatctg ggatgcagaa acaaaaatat
    75001 taaaaatcat cggatgggga atcgctaatt cggatcgcca gcccttgtcc tataagttat
    75061 cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagaagaa gtcaatcaag
    75121 cagaacagct cgcaaaagga acagaagcgg gatttgagtt acgaatcctt tgcgagcaag
    75181 aacgttcatt cttagtggaa gcagtagctc aaaatggcca atcatggatc attgaaatgt
    75241 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
    75301 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
    75361 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
    75421 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
    75481 gacccaaagc ggtttcaagg tacgtttatc agggaaaatg agaggaaaag ccatcttaga
    75541 cttccaaaca gcgagacatc aaaatggcat agctgtgaaa ttaaacggta aataccctta
    75601 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
    75661 ttatgcaagg actcatggcg taaaaaaagc gatccgtcgg gtcaaattgg aattgaagcc
    75721 aggatcgatc gactatgcag agtggatcag tcgtcatgag aaaccagatt tgaaagagca
    75781 aaggaaagaa gcacaaggat ttgcgtaccg tccattgatt tccatactta tgcctgtcta
    75841 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtactggatc aaacgtatga
    75901 tcattgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
    75961 ggaatcctat caagcaaaag atgatagaat caaagtcgtt ttccgaaaag aaaatggaca
    76021 catcagtctg gcaactaatt ctgcattgga aatagcagaa ggagaattta tagcactctt
    76081 agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacgt
    76141 acatccagaa ctggatgtga tctacagtga cgaagataaa atcgatgcag atggcaatcg
    76201 ctttgatccg catttcaaag cagactggtc gcctgataca ttgatgggaa ataattatat
    76261 ctctcactta ggcgtatatc gaacaagcat tgttaaagaa cttggcggtt tccgtaaagg
    76321 ctatgaaggt tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga
    76381 tcatatttac cacattgacc gagtcttgta tcattggcgg acgatcccag gatcaactgc
    76441 aagtaatggc gaagccaaaa gttatatcta tgattccgga gtgaaagcat tgacagatgc
    76501 cctgtctaga agaaacatca aaggaagcgt acgtcctggc agaatttctg gattttatga
    76561 gattgcttat gacgtcttgc aagaggattt ggtcagtgtg atcattccaa cgaagaatgg
    76621 ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt attcaaatta
    76681 tgaaatcatt attgcggata acggaagtac agatccaaaa atgcaagaat tatttgcaga
    76741 atacaagcat cagctgaaag accgctttat cgtagaattg attgacattc cattcaatta
    76801 ttctcgaatt aacaatttag cagcagaaaa agcaaacggc aaatactttt tattcttgaa
    76861 taacgataca gaagtgattg agccagattg gatgacggtg atggtttctt atgcacagtt
    76921 tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc
    76981 aggagtatta ttaggaatcg gtggtgttgc agggcatgca ttgaataact atgatcgaac
    77041 acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
    77101 ttgtatgatg gtcaaggcag ctgacttcaa tgctgttaac gggtttgatg aaacattaga
    77161 agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc gttacaatgt
    77221 ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt atgaagatac
    77281 tccagaaaaa caaagacgtt ttgctggtga aatcaaaaaa atgcaagata aatggccagc
    77341 atatattgcg catgatccat tttacaatga taatttaaca aaagacggaa tcggcgactt
    77401 ttcgttgaga ccagattaaa ctataataaa aagctgttat gttgtgattc gctcgcacat
    77461 aacggctttt tatagttttt tgtcaaaaaa aggcagaagg tgccgactca aaaaaataag
    77521 tcgaggttgg gacagaagtg tttaactccg agaaataaga agaaattcac gaaaattgct
    77581 tttcaaattt ttgtgaattt cggcttattt aagaagaagt tgcttctgct cccaccgttt
    77641 attcgttttt aaagaatgag acaaaagtgt ttcttacttt tgtcccacgc tcttattcat
    77701 gctcagagcg ccaatagata caaaatcata gccaaagttt cacgaaccct agctgtcaaa
    77761 gaatcgatat tttattttaa tataggctat tttaaaataa atggtttaag gtattcctct
    77821 acaaaagcca ctctatcaat tatcatgggt ttaaaataag aagtgatgga gataaccaga
    77881 ttgttcttag cgtcaatata aagaacattt ccactattac caattgctga atatatttcg
    77941 ttctcagaat ctaataccca ccataaatag ccatatctca tattttgtaa gtcttctaaa
    78001 gcactggttc taggtttgaa catctcgttt atccacatgg gtggtacgat ttgttttcct
    78061 ttatacatcc ctttatttag gcataataca ccaatttttg ccagatcact tgccgataaa
    78121 cacaaaccat atcctgccgt cccgatattt agtggatcac aaaaccagac attggtttta
    78181 ggagacttat ccattgtaaa ctgcttatgt tcttcagctg tttcagcata ataattgaca
    78241 tggtttggta tccctatagg atcaaataga tacttattgg caaaatcaac agttgttagc
    78301 ttacttgtct gatacaacaa gccagttaat atgtgtaaac agacagtttg atagttgaat
    78361 tcgccggtta taccttttct tcctcccaat aaatctagtg atgtttttgt ccaatcttca
    78421 cttgaacaga cttttgtcca tggatcgcct ttacatttgt agggtgcttt cattgttagc
    78481 aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt atactctgga
    78541 aaataatcta atactttatc atcgatactt ttgattaatc cttgctctaa agcaatgcca
    78601 atcagcaaag atacaatact tttcgttgct gacatgatat gacaagtatc gtctttttta
    78661 taaccattcc attcatctgt ataaacttct accccatcct tatatgcgac aatttgacat
    78721 atatttggtt gtcgaaaacc aataaaatca tgcaactctt gtttattcat aagatattac
    78781 ctcactttct tctataagca taatgtcatt aaaaatcgat ttcaattttt tcgattcttc
    78841 acgtaataca ggtattctta tggactagac agcgctttgg gaagatggat tctctgaaaa
    78901 atataaaggt tcactttatg agtggataaa aaaagcagca aatacttcga cttctttaat
    78961 ttaccaacat tagtgaaaat cgattcatct ggagatttgt aatttagagt ccttgtcttg
    79021 attagaggtg tataaagctt tttattagac atttttaaat tccattacaa tttgcttata
    79081 aactaggttt ttaaaggtga ctatggtaag ataatagaga tattattttg tgattaggag
    79141 tgtatatatg aataaaaatg gagaatggag tgctgcacga cggatattca ttatattaat
    79201 ggatgttatt gtatacaatg cagcaatata tagtagtttc ttactgaagt ttcgtggaga
    79261 gatcccatta cgaaatttag aaactttcca acactcagca attattattt cagtgatttt
    79321 tattgcgttg aacattttgt tgggtgcata tgttttttat aatcgaatga tcagtgacat
    79381 cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta gggatcatgg tagtgacttt
    79441 tgcgggccgc tggtttgctt ttccacgtct tgttatctta ctttcatttg ttctcggtac
    79501 gcttttctta agtatctatc gtattctcgt atataaactt tatttacgcg taagtcgaga
    79561 taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg
    79621 ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt cttgataact attatgcaaa
    79681 catcaaaaat atgattaaag acatcgatat tgtctatctt gcttctcata tcgaagaaac
    79741 tgaaaaaatc aaaatctatc aattagttac taaaaatgaa aaaaaattgt ttttgaatac
    79801 aacatttgaa aacttgacat tgatcaatcc aaatattatg aatttcgaag atgaaagtat
    79861 tattgaagca tctggattta gaatccaacc tgaatatgaa ctattcaaac gtttgtttga
    79921 tatcatcgtt tcacttgttt tattagttat tgcttcacca tttatgttat tcacagcaat
    79981 tttagtcaaa gtgacatcac cgggaccaat catctataaa caagtgcgga tcacaaaaaa
    80041 tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc gccacagccg aagcaaaatc
    80101 tggaccagtt ttagctaaga gcaatgatgc acgagtaacg cctgtaggta aatttattcg
    80161 agctgtccgc tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg atatgtcgat
    80221 cgtaggacca cgtcctgaac gttctttctt cgtcgatcag ttcaatgcag aaaaccctta
    80281 ttactactta cggcataatg ttcgtgctgg tatcactggg tatgcacaag tctatggaaa
    80341 atatgtctct gattacaaca gtaaattacg ttttgattta ctttatatca aaaaatattc
    80401 attgatgctt gatttaaaga tcttgctgca aaccatcaag atcctatttg ataaaatgtc
    80461 ctctcaagga cttgaagaag acgaaaatgt gatctcattg gcagaaattg cttttccgaa
    80521 tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat tcagcataat
    80581 cgtaccagta tacaatgcag aagcaactat tggtcgaacg attgatattc taaaagaatt
    80641 ggattatgaa gagtacgaag tcgttttagt aaatgatgga tcaacagatc aaacacagga
    80701 aatcattcaa gaactgattg aaaatgatga acgttttgaa ttgattacta cgaccaatca
    80761 agggcccgga ttagcaagaa acgaaggaat aagacagtca aaaggagaat atctcttatt
    80821 cttcgatgca gatgacacgc cagtaaaaac gatcttgtca gattatgatc atttgctaga
    80881 aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa tggacgaggg
    80941 aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt cactatctaa ctcacgaaga
    81001 gtttataaaa gacatgtatc agttaatgaa tgaacagttg atgtatgttg tatggaacaa
    81061 atgctatcgt agcgatattc tgaaaaataa ccaaattcta tttaaaactt ataatagttg
    81121 tgaggatcga atatttaact tacattatta tgaatactgt catcaagtga tcatgaaccc
    81181 aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc aataccgacc
    81241 taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa cagacgatat
    81301 taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta ttttttctat
    81361 ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc agatattgag
    81421 tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag caaaaaaaat
    81481 aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa aagttggaag
    81541 cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat attagttaat
    81601 ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt taggtggagc
    81661 agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc aatttgattt
    81721 tttgatttat gaagattatg cagatgttca agactatcat tcagagattg aaagat
//
LOCUS       DAZAOG010000002        58619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000002 DAZAOG010000000
VERSION     DAZAOG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.002"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1201
                     /locus_tag="ACHDLD_000086"
     CDS             20..1201
                     /locus_tag="ACHDLD_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3361852.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(1261..2889)
                     /gene="treC"
                     /locus_tag="ACHDLD_000087"
     CDS             complement(1261..2889)
                     /gene="treC"
                     /locus_tag="ACHDLD_000087"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN3361853.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(3286..5997)
                     /locus_tag="ACHDLD_000088"
     CDS             complement(3286..5997)
                     /locus_tag="ACHDLD_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN3361854.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(6007..6708)
                     /locus_tag="ACHDLD_000089"
     CDS             complement(6007..6708)
                     /locus_tag="ACHDLD_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3361855.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            6894..7493
                     /locus_tag="ACHDLD_000090"
     CDS             6894..7493
                     /locus_tag="ACHDLD_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN3361856.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7574..8587
                     /locus_tag="ACHDLD_000091"
     CDS             7574..8587
                     /locus_tag="ACHDLD_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN3361857.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            8695..9612
                     /gene="rbsK"
                     /locus_tag="ACHDLD_000092"
     CDS             8695..9612
                     /gene="rbsK"
                     /locus_tag="ACHDLD_000092"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN3361858.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            9640..10035
                     /gene="rbsD"
                     /locus_tag="ACHDLD_000093"
     CDS             9640..10035
                     /gene="rbsD"
                     /locus_tag="ACHDLD_000093"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN3361859.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            10035..11408
                     /locus_tag="ACHDLD_000094"
     CDS             10035..11408
                     /locus_tag="ACHDLD_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3361860.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            11765..13909
                     /locus_tag="ACHDLD_000095"
     CDS             11765..13909
                     /locus_tag="ACHDLD_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3361861.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(13961..15001)
                     /locus_tag="ACHDLD_000096"
     CDS             complement(13961..15001)
                     /locus_tag="ACHDLD_000096"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3361862.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(15278..17080)
                     /locus_tag="ACHDLD_000097"
     CDS             complement(15278..17080)
                     /locus_tag="ACHDLD_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3361863.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(17094..17846)
                     /locus_tag="ACHDLD_000098"
     CDS             complement(17094..17846)
                     /locus_tag="ACHDLD_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3361864.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(17855..18217)
                     /locus_tag="ACHDLD_000099"
     CDS             complement(17855..18217)
                     /locus_tag="ACHDLD_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3361865.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(18393..19265)
                     /locus_tag="ACHDLD_000100"
     CDS             complement(18393..19265)
                     /locus_tag="ACHDLD_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN3361866.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(19291..19803)
                     /locus_tag="ACHDLD_000101"
     CDS             complement(19291..19803)
                     /locus_tag="ACHDLD_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN3361867.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(19941..20732)
                     /locus_tag="ACHDLD_000102"
     CDS             complement(19941..20732)
                     /locus_tag="ACHDLD_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN3361868.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(20787..21395)
                     /locus_tag="ACHDLD_000103"
     CDS             complement(20787..21395)
                     /locus_tag="ACHDLD_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN3361869.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(21388..21705)
                     /locus_tag="ACHDLD_000104"
     CDS             complement(21388..21705)
                     /locus_tag="ACHDLD_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3361870.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(21861..22589)
                     /locus_tag="ACHDLD_000105"
     CDS             complement(21861..22589)
                     /locus_tag="ACHDLD_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN3361871.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            22748..23629
                     /locus_tag="ACHDLD_000106"
     CDS             22748..23629
                     /locus_tag="ACHDLD_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3361872.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(23866..24909)
                     /locus_tag="ACHDLD_000107"
     CDS             complement(23866..24909)
                     /locus_tag="ACHDLD_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3361873.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(24912..25580)
                     /locus_tag="ACHDLD_000108"
     CDS             complement(24912..25580)
                     /locus_tag="ACHDLD_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3361874.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25722..28058
                     /locus_tag="ACHDLD_000109"
     CDS             25722..28058
                     /locus_tag="ACHDLD_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN3361875.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(28173..28484)
                     /locus_tag="ACHDLD_000110"
     CDS             complement(28173..28484)
                     /locus_tag="ACHDLD_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361876.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            28665..30140
                     /locus_tag="ACHDLD_000111"
     CDS             28665..30140
                     /locus_tag="ACHDLD_000111"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN3361877.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            30187..30771
                     /locus_tag="ACHDLD_000112"
     CDS             30187..30771
                     /locus_tag="ACHDLD_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN3361878.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            30773..31192
                     /locus_tag="ACHDLD_000113"
     CDS             30773..31192
                     /locus_tag="ACHDLD_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN3361879.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(31473..31757)
                     /locus_tag="ACHDLD_000114"
     CDS             complement(31473..31757)
                     /locus_tag="ACHDLD_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN3361880.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(32109..32819)
                     /locus_tag="ACHDLD_000115"
     CDS             complement(32109..32819)
                     /locus_tag="ACHDLD_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN3361881.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(32816..34288)
                     /locus_tag="ACHDLD_000116"
     CDS             complement(32816..34288)
                     /locus_tag="ACHDLD_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN3361882.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            34426..34899
                     /locus_tag="ACHDLD_000117"
     CDS             34426..34899
                     /locus_tag="ACHDLD_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3361883.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(35052..35603)
                     /locus_tag="ACHDLD_000118"
     CDS             complement(35052..35603)
                     /locus_tag="ACHDLD_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3361884.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            35708..36238
                     /locus_tag="ACHDLD_000119"
     CDS             35708..36238
                     /locus_tag="ACHDLD_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN3361885.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(36721..37164)
                     /locus_tag="ACHDLD_000120"
     CDS             complement(36721..37164)
                     /locus_tag="ACHDLD_000120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3361886.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(37180..38340)
                     /locus_tag="ACHDLD_000121"
     CDS             complement(37180..38340)
                     /locus_tag="ACHDLD_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3361887.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            38847..40547
                     /locus_tag="ACHDLD_000122"
     CDS             38847..40547
                     /locus_tag="ACHDLD_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361888.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(40649..41482)
                     /locus_tag="ACHDLD_000123"
     CDS             complement(40649..41482)
                     /locus_tag="ACHDLD_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN3361889.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(41786..43951)
                     /locus_tag="ACHDLD_000124"
     CDS             complement(41786..43951)
                     /locus_tag="ACHDLD_000124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN3361890.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            44211..46505
                     /locus_tag="ACHDLD_000125"
     CDS             44211..46505
                     /locus_tag="ACHDLD_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN3361891.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            46498..47163
                     /gene="pgmB"
                     /locus_tag="ACHDLD_000126"
     CDS             46498..47163
                     /gene="pgmB"
                     /locus_tag="ACHDLD_000126"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN3361892.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            47156..48163
                     /locus_tag="ACHDLD_000127"
     CDS             47156..48163
                     /locus_tag="ACHDLD_000127"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3361893.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            48185..49204
                     /locus_tag="ACHDLD_000128"
     CDS             48185..49204
                     /locus_tag="ACHDLD_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3361894.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(49485..50852)
                     /gene="nhaC"
                     /locus_tag="ACHDLD_000129"
     CDS             complement(49485..50852)
                     /gene="nhaC"
                     /locus_tag="ACHDLD_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN3361895.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(50962..52383)
                     /gene="tyrP"
                     /locus_tag="ACHDLD_000130"
     CDS             complement(50962..52383)
                     /gene="tyrP"
                     /locus_tag="ACHDLD_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN3361896.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(52626..54503)
                     /gene="tdc"
                     /locus_tag="ACHDLD_000131"
     CDS             complement(52626..54503)
                     /gene="tdc"
                     /locus_tag="ACHDLD_000131"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN3361897.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(54794..56050)
                     /gene="tyrS"
                     /locus_tag="ACHDLD_000132"
     CDS             complement(54794..56050)
                     /gene="tyrS"
                     /locus_tag="ACHDLD_000132"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN3361898.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(56162..56367)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            56630..57931
                     /locus_tag="ACHDLD_000133"
     CDS             56630..57931
                     /locus_tag="ACHDLD_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN3361899.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(58000..58599)
                     /locus_tag="ACHDLD_000134"
     CDS             complement(58000..58599)
                     /locus_tag="ACHDLD_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3361900.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        17782 a        11977 c        10534 g        18326 t
ORIGIN      
        1 agataaagga ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt
       61 tttaggagga aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat
      121 ttattttttc catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat
      181 cttgccccca ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg
      241 tttgagcaag cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt
      301 tttattgggg cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt
      361 gatcagtagt ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga
      421 gaaaacacct tttgccgatt catttaatca attttttgct tctattgacg acgtgacgtc
      481 tgatatagct ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat
      541 tttttcaact gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt
      601 ccttttgaga gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt
      661 tcgtacagat tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa
      721 ggggcaaatc attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat
      781 cggattagaa tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata
      841 tattgggccg tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt
      901 tatggctgtc aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt
      961 gattcctcga gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt
     1021 acttgtcatg ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac
     1081 gttagtcaaa ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt
     1141 ttatggcgat aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata
     1201 aaacacaaaa cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt
     1261 ttaatttttc agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac
     1321 agtatcggaa taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat
     1381 ccttgtatct ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg
     1441 aagaaagcca taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac
     1501 gattggatat gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc
     1561 agaagatagt tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg
     1621 tttccctgta gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt
     1681 atcgcgagat tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag
     1741 gagtaactta taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc
     1801 tgggtcgatc atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg
     1861 gatcatggtt gctagcatct ctgctccttg ttttcgatac tgatcgataa ctacaaatcg
     1921 attgagcgca cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc
     1981 actcatattt tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg
     2041 agtagaccat ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat
     2101 tgataattct tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct
     2161 catttcaccg actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt
     2221 tagatacgta tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt
     2281 atttttcaat tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac
     2341 ccctttttcc atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg
     2401 ccagttcaag tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc
     2461 aaatggcgac caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa
     2521 aatgaagaag tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc
     2581 tgtcgaaacg tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc
     2641 ggctattagc tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc
     2701 agagatatca taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat
     2761 gtcgatgcct agatcattca gatacgggag tttttctttg attcctttta aatctccaat
     2821 tccatcacca ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt
     2881 aaatgtcatg gatatttacc tccattcaac tgatttatta cctaccacag cttatatata
     2941 caagttatca tttattatta taactgaaca gaaagcgttt gtaaaattta tctttataca
     3001 ttctaagagg tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc
     3061 ttgtttaaaa tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc
     3121 tacggcaatc agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt
     3181 tttgctcgat gcaggacggc ttttttacaa cctctgattc atggtgttca attagcagct
     3241 gaccctcgtt attagctcaa aacgctgatt tcacaacctc tgtttttatt cattcgattt
     3301 caacgcatca atcatatcga tatgctttaa tttgaaatgg gtaacaatca tcacaataac
     3361 cgtaaatacg atagttaatc ctgctgataa gacataagcc caccatgtga tcactaatgg
     3421 aaagatgaca ttctccatag acgcttgttg caagatgaag tcagtcaata tatatcctac
     3481 accaaatcct ccgatgattc ctaaaagagt aaagatgata ttttctcgaa caatgtacat
     3541 cgtgacttct ttatcaaaaa atccaaggac ttttattgta gataattcgc gtacacgctc
     3601 ggaaatattg atattagtta gattatacaa gacgataaaa gccagcagtc ctgataatac
     3661 gacaaagatt aagacaaccg agtctaagtt cgccattgat tcttcttgcg tttcgatttg
     3721 tttggacatg aaagacgtat ttgtcacagc gtctgtggca agcagtttct cggaaagcat
     3781 ttcttcttct tttgttgtca tcttatctgt acgcagcaaa aatgcattat ccatcatcga
     3841 ttgatcagta actgaagcat aatattcagg ggacatataa ataaagctac ctagatagtt
     3901 tttcacgatt tcttttactt cgactttcca ttctttttga tcttcatcat aaagcgaaat
     3961 atgatctcct gtagataagt caaatagttc tgctactttt tgcgtaatag cgattcctgt
     4021 tttaggcaag gagcgtgggt tctcttcaag atcagttaaa tggataaaac gatccaaatc
     4081 agctgtttta cttggtacga tcactgtcag actttgcgaa gcatgtcctt tttttctcaa
     4141 ttctacggtt tgagtatgaa tagctaggat ctcatttact ttttcttgct tctgcagcat
     4201 atccgctgct ttttgacgac tatcttccgt ggatgaatcc aatgtaacga ttgcttgata
     4261 gtcaatgatc ggtccaaatt gtttggctga aacagaactt agagaatctt tcaatcccac
     4321 gcctgcaacc atcagcccgg cacatccagc aattccgata atcgccataa gcattcttga
     4381 cttatatcgg aagagatttc ggtaactgat tttttgatta aagctaaggc gtgaccacag
     4441 cggagtgatg tattcaagaa aaatgcgttt ccctggcttc ggtgctttcg gttgaagcaa
     4501 tgcagcaggc cgttctctta aatctttgaa taaaacgatc attgcagcgc caaatgcggc
     4561 aatgaagaat gcggcagctg cttgtaaaat cggcggccag tcataaaaaa taagcgcact
     4621 cccaatatca taccgctcgt tagacagctc aaaaatgatc cttggcaaaa gattcgttcc
     4681 taatatcgtt cctaaaacaa tacctatccc tgaggctaga cttgcataga ttgcatattt
     4741 ttttgcgatt tcaactttcg tataacctaa cgcctttaat gtcccgattt ccctgcggtt
     4801 ttcttctacc atacgtgtca ttgtcgtaaa ggtgatcaat gcggcaataa agaaaaagaa
     4861 aacaggaaaa acattggcaa tcgcagcgat acgatcagac agatcaccaa attcttgaaa
     4921 accgacattt tctgaacgtt cattgtatag gtatgatggt ttagttaaat tttcaatttt
     4981 ttctgcattt cgattcaatt cttcctctgc agaggccagt tcacgttctt tctcaactaa
     5041 ttgcctttct tgttcagcaa gttgagcttc ttgttgaggt aatgtttgaa cagcgattgt
     5101 tcgttgcgct tcaggaagct gctggatagt tgctgactgt tgcgctaact gttttttagc
     5161 atcttctaac cgtgttcttg cttgcgttgt ttgtgctttg ccatccgcta ttttttgctg
     5221 tgccggttct aattcttcgt tggccgattt tttcagctct tctaagcgtt cttcgggtcg
     5281 atcttgtaaa tacttttctg ccttttcttg attttgctcc attttatctt tgtaagcttt
     5341 gctgtacgta ttcaaattct ttacgtttgt aaaatcaaga tataagacag aataaatatc
     5401 tgttttaaaa acggattcag gtaaatagac aaaataatcg acgctaccat ttccgacgtt
     5461 cgcgtatcct ctctccaaat tgttgatgaa caatggagaa cggacaaagc ccgtgatggt
     5521 atactctttt tctttcaatt catctgaatc aatctgataa atatcaccga ttttataccc
     5581 ttcatcttcc gctttttcat ccaatacaag ctggtttggt ttttccggga gttttccatc
     5641 agttaattgt agcttgttct gcttgttatt tttgttataa gaaaatattt gaatcacttc
     5701 gtttttgcca gtattggaat aatagatctg ttttcctgtt tctacttttg catctgggat
     5761 cttttctgct tgagcaatat ctttttgtgt caagccacca gtagaaacga tttgcaaatc
     5821 agaaagtcct gctgtaccaa catagtgatc catcgttttt tgcatgacag gtccagcagt
     5881 tttgacacct acaaataaaa gtgttcctag catgacaata aggatgattg caatgaatcg
     5941 tcctttagat tgccgaattt cgcgaaaacc tgctttaaga tatgttcttg ttttcataaa
     6001 atccccctac catacaatct catcaatgga tgaaggatga tcatttaatt caacagacct
     6061 tactttagca tcattaatgt gaatgactct atcagcgatg ggcgctaaag cagaattatg
     6121 tgtaatgatc aaaacggtat tgccgtgttg tctgctagcg ttttgcaata atttcaatac
     6181 ttgcttgccc gtttcaaaat ccagcgcgcc tgtcggttca tcacataaaa gaagtttggg
     6241 atttttagct agtgcacgag caattgaaac cctctgctgt tcgcctcctg ataattggga
     6301 cggaaagtta ttcaatcgat gagctaggcc cacttgcctc aatacctcta caggatcaag
     6361 cgcatcagga gaaacttccg ttgccaattc cacattttct tttgctgtta gattaggaac
     6421 gagattgtag aattggaaaa cgaaccctac atctgttcgt cggtaagcag taagctgttt
     6481 atctgaaaat tgcgcaatat ctgtgtcatc aataatgatc tgaccttcat caggtgtatc
     6541 catcccacct aaaatattaa gaatcgttga ctttcctgct ccacttggac caagtataat
     6601 cactaattcg cctttttcaa ttgaaaaaga aatatcgtca tttgctgtaa tggtcgtttc
     6661 tcccattttg tatcgtttgt attcattttt tacttcgaca tacttcactc tgattcctcc
     6721 attttcacga tatttctcgc tatatctatg catcgaatgc gttttctcac acttatttat
     6781 tttaccactg aatttctcta gactaaagaa aggaatcctt taatttagtt gacaaataaa
     6841 cgatttatga tttacttaca taaaaaacat tgttatttga aagaaggaac atcatgaatg
     6901 atttaaagga aaatatgatt tttgatttat caaaaactgc ttttgtcgcg atcgacatgc
     6961 aaaaagagat cgtaaataac ggtacactct ctccacacac tgcagcatct attctgacaa
     7021 acaatgattt gttagtaaga acattgaaac gtaccgctgc cttgaaagtt ttagtgaacg
     7081 tagacatctc cactttccct tatttgagtc aacaaactga tatgggtgga atgactgctt
     7141 ctgtacctcc agaattcact gatttacttt tgaaggattc tttgaaagat acagataata
     7201 tgttgactat caaaaaatac aatccaagtg ctttttttgg tacttctctt gatcttcaac
     7261 ttcgccggcg tggaatcgaa actatcattt taagcggtgt agcgacaaca aacggtgtat
     7321 atgctactgc attagatgcc tttcaacacg ggtaccatat cgtgcttgct gaagatgctt
     7381 gcagtgatcg tgacaaagaa tcccatcaat tgttcataaa aaagatattt ccgaaaactg
     7441 cacgagtacg ctcaacgaaa caaataatcg aagctatcca acaaagcaaa taagtactgg
     7501 cacacgtgat tttcatttac ttctttttga tttcatggga aaataaccac aggatttaga
     7561 taggagtatg aacatggtga aaaaaaagat cacaatcaaa gaagtcgcta aactttccgg
     7621 cgtttcaatc acaactgtct ccttgatatt aaatggaaat acggagaaat tcagtccgaa
     7681 aactgttcag aaagttttag ctgccaaaga agaattaggc tatcagcctg actattttgc
     7741 tagacaaatg attaccaagg aaacgaaaac aattggtgta ctcgttcctg atatcacgaa
     7801 tccattcttc agtacactga tgcgaggaat tgaggatatc ttgtataagc agaatttcgt
     7861 cacgatatta tgtaatgctg attctgatca tcaaaaagaa atcgaatatc tagccgagct
     7921 cacacgtcga ggtgtggatg gattcattat cgcgacttct gctgtctcta cagatgcaat
     7981 caacgaaaat ttgaaaaaac aaggccggcc cttcattgtt ttggatcaga aaaaatctga
     8041 aggatttagt gatgccgtac gaacagacga ttttcgcggt ggttacctag cgggcatgca
     8101 cttgcttagt ctaggacatc aaacgatcgc ccttgtctat cctgaaaatc caccggaaaa
     8161 tgtccacgct cggattgagg ggtttaaatc tgctcttgat gtttaccaaa tccctcacga
     8221 ccaactgatc ctcttaccta cacagttctc aaaacaaggt ggctaccaga tcacagcaga
     8281 acttcttgat tctgctgcaa ctggtgtgtt tgctttaaat gatgaattag cttttggatt
     8341 atatcgcgga ttagaggaag ctggaaaaag catccctgaa gactacagca ttattggcta
     8401 tgataatata gatatgtgcg agtatatcaa gccgaaactg acaacgatcg cacaaccaat
     8461 cttcgaattg ggacaaacat ctgccaagtt attgttagat cggatccaat tcccagaaaa
     8521 agaatgggaa gaaaaaagat tacctgtacg attagaaaaa agattttcaa ctgcaccctt
     8581 gaaatagctt tcaaggtgtg ctatattttt atcgaactat taaaacgttt tactaaaacg
     8641 ttttaaaaaa taattttccc atcaaaataa atgagatagt tggaggtttg taaaatgaac
     8701 acggttacag taattggaag tattaatatg gacactaccc ttcgtttaac caacatgccc
     8761 aagccaggcg aaacgatgca tgcgcatgag atttttcacg ctggcggagg taaaggtgcc
     8821 aaccaagcag tggcagccaa acgttcaggc gcacggacat cttttattgg aggcgttggg
     8881 gctgacagcg aaggacaaca gttattagat ctactgacaa aagaaaacat cgatacatct
     8941 gggatagctg aaatccaagg cgccacaacc ggacaagcaa tgattatggt agacgcagca
     9001 ggagaaaata gtatcttgat ccatgcaggg gcaaataatg ctttccatga acaagaagtc
     9061 ctaaaaaaca agcaactgat tacaaacagt gactttatta ttgctcagtt tgaaagtagc
     9121 ttagatgcga cgatcctagc tttttctatt gcaaaagacg caggaaaaac aacgatcctg
     9181 aatcctgcac cagctcgtga aacgattcct actgaactac ttgaaaagac agatatcatt
     9241 ataccaaatg aaacagaaac agaaatcatt acaggaatca gagtgacaga tcataacagt
     9301 ttggtcgcag cagccgaaaa actccatgaa ttaggtattg gaacagtaat cattacacta
     9361 ggcagtgccg gcgctttcta tcatacagaa aaagaacacg gtatcgtccc tgcttttaaa
     9421 gtggatgccg ttgacacaac agccgcaggt gatacattta ttggcgcatt aagcagcaca
     9481 cttcagccag accttagtaa cttgaaagaa gcgatccttt atggaaatct agcctcttct
     9541 gttgctgtgc agtcttatgg tgcgcaacct tctatccctt accgcgaagc tttagaacaa
     9601 tcagcagtct aaaattaaca atgagaggaa gaaacaaaca tgaaaaaagg aaaagtaatc
     9661 aattcagata tttcacgagt aatcgcacaa atggggcact ttgataaatt aagtatcggg
     9721 gatgcaggca tgcctgtccc ctttggtact gaaaaaatag atttggctgt ctcaaacggt
     9781 cttccaagtt ttttatctgt tttagaaaac gttttagaag aacttgaggt acaatcgatt
     9841 gaattggcgg aagaaataaa gacaatgaac ccggaaatat tggaaaaaat caaattattg
     9901 ttaccagata caccgattag ctttatccca catgaagaaa tgaaaaaaga gttgaataac
     9961 tgtcatgcat ttattcgtac aggcgaaatg acaccttacg ccaatattat attagtgagt
    10021 ggagtggtct tctaatggaa acgacaatga atcctgaaca gatggtactg tccgagcaga
    10081 ttcatatgct gattgaacag ttgggaggaa aagacaatat tgattctgtc cttccatgtg
    10141 cgactcggct tcgtatcaaa ttgaaaaaac agccagtagc caatgcagaa aaattaaaac
    10201 agcaaatccc ttccattaag tatttcattt tactcgatag atatgcagaa ataatcatgg
    10261 atcagggatt ggctcctgct tacaaggaat tgacgcgtca aatcgatcat tcagctccga
    10321 aagaaaagct aaatccgatc gaaaaactat ttggtttgat atcggaaatc tttacgccga
    10381 tcattccgct gtttgccggt tctggtatat taaaaggtct ggttgtttta gcgactacga
    10441 ttggctggct tagtgaaaca agcggaactt atcatatttt gtctgctgcg tcgaatgccg
    10501 tcttttattt tctgccaatc tttcttgctt tttctgctgc caaaacgttt aaagtgaatg
    10561 gatatattgc ggctgtcatt gctgcagcac tgattgaacc gaatctgacc tctttacttt
    10621 cagacagtgg caaagcagct actgattttc tggggattcc tgtagtactg atgcagtata
    10681 cgtctactgt catgccggcg attttaggta ttttctttta ttcttttgtt gaaaaattcc
    10741 tcaaaaaata tattcgcgaa aatatgcaat tagtatttgt acctttactt tcattagtta
    10801 tcacggttcc tttgacgtta atggtattcg ggcctgcggg tgtttacctt ggcgaaggtt
    10861 tagctgccgc aattacttgg atgatggata ttaacggacc gatcagtggc gcaatcatcg
    10921 ctggtggttg gaatattttg gtaatatttg gtatccaatg ggctgtaaac cctgtcatga
    10981 tcagtaatat ctctgcttat gggttcgacc gaatcgttcc gttgacagga gcagccaatt
    11041 ttggaatggc tggtgcagct ttaggtgtat ttctgcgaag taaacgttca aaaacccgtt
    11101 cgatctccgg ttctgctttt gcttcgattt tacttgcggg agtgaccgaa ccaactgttt
    11161 acggaattgc gatcccattg aaaaaaccat ttgttgctgc ttgcattggt gcagctgctg
    11221 gtggagctgt aatgggattt gcccaagtga aagcgatcgc ttttgtattt ggtagtctga
    11281 ccacattgcc tgcattcatt tccggtactt ttttctggta cttagcagga ctggctgtta
    11341 gtctagtcgt agcaatgatc actactctcg ttagcgggtt tgatgaagac ctaatgtctt
    11401 atgaatagtt aatgaaaaag actgaagtat gagatgttta tcacatattt cagtcttttt
    11461 ctttttagga tcattatttt tataaatttt tgtgatactt tccaaacttt ggtataattt
    11521 acatatgcat aaccattttt atccccactt gtttatcccc aaacaaaggt ggtctatgca
    11581 ataaaatgaa cgtcaaagaa aaacgaatga atgctcgttg cttatttaca aattgtttta
    11641 tttagaatac acagacagat ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc
    11701 aaaaaatgtc taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa aggactagtt
    11761 tattatgaaa aataaaaaaa cgatacgttt gctagctgct acttggctga ttgctccgtc
    11821 tgttttgtcg accaataccg tatttgccgt agataatcaa atagctgatc aaacagtcac
    11881 aacagatacg actacatcca gctcagctac tagtgaaaca acggattctt ttacagaaga
    11941 cccaactgga acaactaact caacaacagg atcgaccgag ccaactgaaa ttgatccggc
    12001 tgatccatca gaaaatgaaa agccagccga gacatattcc cttacaattg ctccttcttt
    12061 gattggtaag atccaactga ctgcttcgac agcggattcc cctgaacagc aacctttgga
    12121 tattacggaa agtggaacgg tagaaggttt attagcggaa acgaaggtaa cttacactat
    12181 tacgccagct gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa
    12241 ttatgatgca aagaccttta cagtaggtac gaacaattta tctattactg cagaatttaa
    12301 agctggaccg gatacttcaa cagagaaacc aactgatcct agtgaagaag tccctagtac
    12361 accagatccg gaaacgccaa acaaaccaga tgtgggtaat aacaacaata ataatggttc
    12421 aaataattca aacaataata acaataattc gaacagcggg aattcttcga atcaaacaaa
    12481 cccatctgga tcgaataaca atggaaatac tcagagagaa ccaaaccatc caaacaatgg
    12541 ttcatcgaat ggcggaacga acaatgcttc atctcgtcca aatacagcaa atcaaagcgg
    12601 tatagagaat ccatcttctg cttcttctga ttttgttgta aaatcaccaa ttgatgctgt
    12661 attgcctaca aatacaacga atgtccaaca agcaatcgtt cgagaagcat tcaagcatct
    12721 tggaaaacca tatgtatggg gagcaaaagg tccaaatact tttgactgtt ctggtttgac
    12781 ttattacgta tatatgaaag caaccggtca ttatatcggt ggctggacag gcgaacagca
    12841 atatgcaggt acgcagattc cagtaagtca ggcccaacct ggcgatctcg tcttctgggg
    12901 accttcttcc ggtgttactc accatgtagg tatctatatt gggaacggac aatttatcca
    12961 tgcgcctcaa ccaggagata aagttcgagt aacttctatt tcagatttca cgcctgattt
    13021 tgctgtccga gtaaacttag caggtttacc tgctgcttca ggttcgctga caggcggctc
    13081 atctatttta gatggattag atggctcgtt ccatttcagt caaaaccaaa caactgatca
    13141 gttcttgaaa aagattgcag atgatgcaca ggaaatcggg caaaaagaag gaatctacgc
    13201 atcagtcatg atggctcaag ctatccttga aagtggatct ggaaacagct tattatcaag
    13261 cgaaccaaat cataaccttt ttggtatcaa aggatcatat aaaggaagca gcgtgacctt
    13321 caatacactt gaacaagata gttccggaca aagctaccaa atccgcgcac aattccgtaa
    13381 atatccttct tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa atggactgac
    13441 tggcaatcct gatttctaca aaccaacttg gaagtcggaa acaaaagact acaaagaagc
    13501 aaccaaatat ttggaaggac gttatgctac agatcgccaa tattcacaaa aattgaacgc
    13561 gatcatcgaa gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac
    13621 tgaagaagca aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg
    13681 gacaacagat ggcgtgccaa tcacaggatt agaattttca acgataaaac gcgtcaacaa
    13741 cgcttcttcc atcttccgtg tcgcttcaat ctgggatttg tggaacaatt ttacggcaag
    13801 acagatacca gaaacaacta ctcgtacagt taaagctgca actgttcctg tactaacctt
    13861 gttaagttta gacaaatcat tctggcttga tctgccttta aagaaataga ataaattaaa
    13921 aaggggatgt gacactgtca cactcccttt ttaatttatt ctatttcttt atatttattt
    13981 tgtattccgt tacaaactcg cgtacttggt taggttccag agtgacatct ccaaaacttt
    14041 caagtcgctc cgctcctggc agttcttgag tctcaaatgt gataccgcca tgatttacta
    14101 atttattccc gcgcatttcc ggtccatctt ctccaaaatt tgctgtaaaa atcacgattc
    14161 ctgaagcatc tgtttttact tggatcgata ctttttcatc tgggcttgtc aattccgcag
    14221 catttagtaa accagattgt tttttcaaca aaaatggatg atcgatgccg cccactatca
    14281 ttgattggcg aaaatcactg gcaaagacct cacctaattt tttaggacgt tggaagtcaa
    14341 atgctgtgcc acttactgaa actacttctc ctgtaggcgt tgtatcttta ttgagcgtag
    14401 caaataatcg gctattcaac caaagtatat gttcgtcgat tgactgtgct ggatcaccgg
    14461 ttaaattgaa atagacatga tttgtaggat tgaatactgt tttgcggtca ctgatccctt
    14521 ggaccgtcac tcgccatgta tcatcgttcg tcagcgtata ccgtacttct acatctaaag
    14581 ttccaggaaa tccgtttccc atatctgggc tagtctgata gaaaatgacc gatgcttcgt
    14641 tttcaccatt gatgactgta taatgccact gcttggcttc aaagctaggt gcaccaccat
    14701 gtaaacaatg accagtgtcc gggtctgttt ttacttcgaa cgtttcatca tctaatttga
    14761 atgtgccatt cgtaatccgt ccagccatac gtccgattga agcaccgata taagggtctt
    14821 tttctagata ttcttttgct tcatcaaaac ctaagatcaa ctctcgtttt tcattatcga
    14881 ttattgtttg aaaatgaaca acccgtgccc caagatcact gacagacaaa gatgtcccat
    14941 tttgattcgt gatcgtaatc aagtgatagt tttcgccaaa attttgttca ttgatttcca
    15001 tgttccttgc accctctttt ctttcatttg cactgattcc agtatacatg gaaaccgttt
    15061 tcttaacagc ttattccgtg agtaacgaga aaaaatttac tatattctaa taaaagaccg
    15121 tgaaaaaaag gttgtgacaa aaatattgtc acaacctctt tttccgaaca aacggtgttc
    15181 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagga gctatttttg
    15241 cttggctgtt cttattactt gatgcaggac agctttttca caatctcttt ttcaatacga
    15301 ttccagtata taaccatgtc gtcagtacat agtagatgaa atacaatacg aagaagatcg
    15361 taaacggaac ccaaatatct ttgtatggat tttgcatgaa tagaccaaac atttgtaatc
    15421 cgaataagac atggactaca cctaagattc ctggtgctaa gaataacacg cctacttctc
    15481 tacggatcga ttgtttaagc aacccgcgtc gtgttcctat cttttcaagc atttcataac
    15541 gagcaatgtc actcttgctt cctgacaaga tcttgaacat caaacagcta gcaagcatgg
    15601 tcaagaaagc taaacctagg aagaatccca taaattcaaa accagagaat agaccattat
    15661 acacttcata catttcatat ttctggttgc tgtactctac tttttgcaaa gatggattgt
    15721 tcattttatt ttgagcaaca agtgtttcga ttggtttcag gtttgcttgg aagtctttta
    15781 cctgaactag ctgtaaagtg gattgtggca agttcagttg atcaaattct gattgactga
    15841 ctaccttgat ttctttttct tgctgttccg gcaattcata gcttcgaagt tcctcttgta
    15901 cagtcagttc attcattttt ttagcaggaa ctttttcttt cgtgatcttc ggcggtgttc
    15961 catcttcatg ttttatcatg atcaatggtt gagccgcaaa ttcacttgca ttatagtaaa
    16021 tcgttcccgc atcttctttt tgagtataag aagcagaaag tgttggtgaa agttctttga
    16081 ttttttgctg atcttctgcc cgaggattat tcatcactag atcatatcca gaattgtttt
    16141 ctgtcaagtc tgcaatttca tttttaaaac ctaatcctac cgtcaaagcg cccagtgcca
    16201 aggcaaacag catagcgacc attgagagca tttgtgtata ttcacgaata cgaaagttta
    16261 actgtgacaa tgtgaaattg ttcaattttt tcaatgaaat ctgttctgat ttctttagta
    16321 atgataaaaa gaaaatgatc acagaatgga aaatgaaata agttcctaat atgatcgtaa
    16381 ctaaggctac ccctatacca aagacttgat aggtccccac gtgtttcatc atatagtaac
    16441 cgcagcctag aaaagcaatt cccagtacta cttcaaggaa aaacaagaat ttatgctgct
    16501 tgattcttgt tggtgtctga tccgcacgaa tcaatgctaa aatcggtttt ttgacaatcg
    16561 aaaaagcatt gacaacagca gctaataaaa acaaaacagc aaaaaacaaa acagtgatca
    16621 acaacccgtg aatactaaaa ggtgtaaaat gcgaaatatt gagatctaac tgatccacta
    16681 ataatcggtg gacgatactt gtcaatccta caccgacagc tgaacccact aaagtagctg
    16741 cgatccctac taagaatgtt tcagaaaaaa tcatctgtgc gatttttctg ccttttgctc
    16801 ccagcatcat aaacattgca tagtcttttt gccgcatcgt catcaaaaag gaatttgcat
    16861 ataaaatata aacgaatgtg atgatcccta gtaaaacaga accgaatcgg aaaatgataa
    16921 ctgtagccgc aacgatcgta ttacttttca aaaaagcttc gttgcttgcc attgattcaa
    16981 acatgtaaaa aatagcagat gccataacta atcctgagaa taacaccaag tagtctctta
    17041 aacggctctt gatcccagta agtgataatt tccaaagcat agtcaatctc cccctactgt
    17101 tccaagtttc caagaataga tagtatttct cgatagaagg aatcacggct ttgatctcca
    17161 cgtttcactt cttgatggat accgccatcc ttgatgaata aaatacgttg gcaataactt
    17221 gcagaaaacg gatcatgcgt cacaagaaga atcgatacat gatcttttgt attcaattcg
    17281 tccatcgtat ctaaaagatc ccgggcactt ttggaatcca gcgcacctgt aggctcatcc
    17341 cctaatagaa tcgttggttg tgtgatcaat gcacgtgcgg cggcgacccg ttgtttttgt
    17401 ccaccggaaa tctcagaagg atagctttcc aaaatatgag agatggataa acgttctgca
    17461 attttttgga cacgttgctt gatttctttt ggtctgacgc cttgtaaaga aagaggaaca
    17521 gcgatatttt cttgtgctgt caagctttct aataaattga aatcttggaa aataaagcca
    17581 atctcattcg cacgaaaatc tgctagttga tttcctttta atgtggtcac atctttttga
    17641 ttgattcgga tatgcccatc tgttggttta tctaatgtgg ataaaatatt caaaagtgtc
    17701 gacttaccag aaccagaagc gcccatgatc ccaataaatt cgcctttttc tacatcgaag
    17761 ctgatgccgc ttaatgcttg cgtctttttc tcattgtttt ttccgtatac ttttgttaca
    17821 ttctgtactt cgactatttt gttcatttct ttccctaccc tttaattagt ttttccagtg
    17881 ctgttttcgg cacgtctttt ttttcgactt cttcatacga agtgacacct ttatttttgt
    17941 tccaggtgat ttttaaatat gctcctttac gaagcggtcg agtcaattgt cccttgaagt
    18001 ccagttcttt ttccttaccc tctttcgtat agccaggcag gatatatcta tagttgactg
    18061 tttcttgtcc agagtctgat tttgaaactg tttttgtacc atctgtcgta atttgtacat
    18121 agtattcttc tccacccata actacgtaat ctgcgatttt cacccctgca agtaccacaa
    18181 cgatgaaaac aagtagaccg atcagaagtt ttttcacttt ttccccttct ctcttacttt
    18241 atatactgat tatagaaaaa agcgaacaat tctgccttcg aatcaactat cattcttctt
    18301 tcaattttgt cattttcttt aaaacaacta aaaaaagagt tcaagacaat aatccgtcct
    18361 aaactccttc ttcctatctt acaaaaagta tgtcacttac ggtctttcat gtcgcctgta
    18421 ttgataacag gttgcgttcc tttgtctgta atgattgaga caagtaaatt attgatcaat
    18481 tggactttcc gatcatcggt gaaattgatt tcttgtccat cttcgatttg ctctaaggcc
    18541 atttgagtga ttgtgacagc tccttcaaca atggtttgtc gagcagacaa aatggctttt
    18601 gcttgttgac gttggagcat ggcactagcg atttctgttg cataggctaa atggttcaag
    18661 cgtgtttcga ttacttcgac acctgctact gctaaacgct cttgcagctc tttttgcaac
    18721 tcgtcagaca ctgccgtcgt gtttcctcga agcgtcaaat cattctcatt gaatgtatca
    18781 tacgggtatt ggctagcgat atgccgaatg gctgtctcac tttggatttc tacaaaatct
    18841 tgataatacg ctacatcgaa taatgccttt gctgtatcaa cgactttaaa gactactact
    18901 gctgagattt cgatggggtt tccatccaaa tcattgactt ttaaaacaga actattgaaa
    18961 ttccgaactt tcaacgaaac agtcattttt tgtgccaaag gtatcgtaag gaaaaagcca
    19021 ttttctcgaa tcgtacctaa atattgtcca aaaaacaaaa ttacttttgc ttgattagga
    19081 ctgactacag ttgccgaact taacaacaat aacgtgatca accaaagaaa aatacttaaa
    19141 aaaagtgccc aaactttcgc ttgccacata cctaaataga agaaaaacaa tccaactaaa
    19201 gctaatacga ccaatccgac aatccctgca taaccattga tataaaatgc tcttttttct
    19261 ctcatgctac ttcctgcgct cctttatcct tcaataaata tctaatgtta cttttttaat
    19321 tttttttcga atacgcgcca ttacaccttc tagttctaac tctttgtctt cagtgatgta
    19381 ccaacccctt gcctgtgtcc ctccggctgc ctgcggtaaa taatccaatt ttgttcgaac
    19441 ccagtgcttt tcttctgtaa aatagtcttc taccaagacc tctagatcca tccctgctga
    19501 aagaggcaaa tcttcccgaa cagatttaaa gataaatcgt tccttcttcc catcatactt
    19561 caatattcct gtatcgataa tcggtaaaga ataataatcc agctctttct tcaccgtcaa
    19621 taaatgtttg aaaacttcat ggtacacttg ctctaattgg cgaccttctg aattgttcgg
    19681 gtaattaacc atcacttttt ctttgtctgg atcatagaag gtttggctta gttgagtgat
    19741 ataagtatgt aatctttcca tttctgtttt taacttttct ctttgacctt cttctacctt
    19801 catatactct ttcaccctgt tttcttttta gatattttct ttcttatttt actatagtta
    19861 tgtgcgttaa gcgatgaaaa acagcaaaga ttggaaggat ctttgctgtt ttttcttaaa
    19921 atatccagtt gagtacagga tcaatctgtt tcattgtcct cttgatccgt cgtaatattg
    19981 atcgatgtgt ctttagcagt gatctttagc tttttctgtc cttgttctaa caaagtattt
    20041 gttattggct ggtagtcttt ttcaatataa gattcaccag acgtttggat agtatagcca
    20101 ctgttttcca tctcatgcaa agataaattg ccaccgttta ggatgacttt tccttctttg
    20161 ctgatcaaac tgtcttctag atggaagttg ctgttttcac ctgtgacgat caagttatcc
    20221 acattggcct catctaatcg aagtgagcct aattcatctt tcgcagttat tgttcctcct
    20281 gtaaattcat tgaacgatac ccagccatca tgtgtcttag tattcacttt tttgattgat
    20341 acttcgttga tatcaattgc actattattc ccttcgattg tcaattcaga taattcatga
    20401 tctgcaggga caacaatagt gatttgtgca cttgtgtctt ctaaactaat gattgcacct
    20461 gttgattttt tgactgcatt cacagtcaac acgccatttt tctcctttat ttctggcttt
    20521 tctgtcttat cataagtcgt ttttatctca aaatccaatc cgcgtatgac cgaaactgtt
    20581 tgattttctg catggatgac gattttgttt gcatccttga ttttttttgt ctcagtaacc
    20641 atatcgatca accgaggacc attatcccaa gtcagactct tttgagcgcc taatgtataa
    20701 gttgcacctg ctaggaggcc accagtaagc atcagaccaa taccggtaat caaaaaatgt
    20761 ttcaatctca atttatgtgt cctccttcaa acgcccgctg aatgcagatc tctttttgta
    20821 ataaaacgtc gtcccatcca tttaacaaag ttcatcatga cataaaaaag cccttttgtt
    20881 cctttcagca acagtggcga aataaatact gcgcttcctg tggcgaacag accgacaccg
    20941 ataaagaata atccactcag aatcgattgt ccaaacacac tgattcctga gatgatcatg
    21001 aataggccaa cacctaagat actgatacat aaaacataga tactaaaaat gagcgctagt
    21061 aaagcaacca atagtccccc aataaccaat ataaacccca gtgctagtgg aatgaggaca
    21121 ggagaagcaa ataaaccgat aatgattaac caaatcaggc gaatttgtct ttttggattt
    21181 ttcactaact cgttttctgc ttgttcttct tctgaaaagt aactgatttt taattttaag
    21241 gcaaactgct ttggtgtacc atattcatca atgatctgat ctaatgtttt tccagaatct
    21301 aaaaaatatt cctcataata ctggatcaaa tcaaaacgat cgtcttcagg aatttcttga
    21361 agatgatcca caacttcatt caaatattca ttcatttgtg ctctcctcca ataaccggtc
    21421 taaagaatct cgaaaaacaa tccattcatc aataatttct tctaaatgac gacttcccat
    21481 tggtgtcaac gagtaatatc gacggtttct gccttcaaaa ggttcatcat aggtctcaag
    21541 atatccttct tttttcaatc ttctcaaaac cggatatagt gtagattcac tgacagagag
    21601 actttcttgt acgatttttg ttaaggcata cccatacata tcttccttgc ttaatgctgc
    21661 tagcacagta ccatcaagaa gaacagttgg aatctggatg tgcattacgt caccttctat
    21721 tctcaagtgt aatattttat ttcaatctat attatacaac atataatata gtttcaaaat
    21781 cagtttgtca aatacaaaaa aactgactaa aagatttgat cctttttagt cagcaaaaaa
    21841 tggcttctct ttgattttta ttataacagt ggcataatcc aatttgtcca catccagctc
    21901 agtggcatga taacgatcat tgcaggtatc atgtcagcta ctgggaacat tttcacgttg
    21961 atcattctaa aaccggtagc gaccatcaag aatccgccgc aagctttaaa atctaagatc
    22021 atgtctggtg tagtcaatgg ataaataaat tttgccaata agaacaaaat ataaaaaata
    22081 atgaattggg gaattgctat cacagaaacg acatacccta aattacacgc aaaaatagct
    22141 gctgtgaaaa aatcaaggac agatttagaa atcaaaatcg tactgtctcc tgtcatccca
    22201 gaatctaaac ttccatagat ccccgtacca cttgcacaaa atagaacaat gaccgtcact
    22261 aatgtgctga taaattcatc gtgggtcata ccaagctttt cactagggaa gattttagaa
    22321 ataggtactt gcatcagcgc agccccctta tttatcccat tacctaaatg aacaatcaga
    22381 ccaatcgctg ttcctatcac taaagcaaaa ataacagccg gcatattttt catcggcgca
    22441 atggaataga ttcccatccc catcgaacac acaccaaaaa ccatcgtcag ctgtgcttta
    22501 aaatgttcac ttaatttatt tcctactaat ccgccaatta gtccgccaag caaaacggaa
    22561 cctacattga tcaaaactcc agttggcatc aaaaaactcc tctctttttc tacgaagcga
    22621 aaaaaatcag aaaaatttct gtaattattg ctttcgctgc aagaaagatt gtaacagaca
    22681 gagaagatta caccaattca ttttttgtgt taaagtattc tttattggaa taagtaggag
    22741 gaatatgatg gatatccata aattcgaagt ttttcttgat ttagccgaaa caatgaattt
    22801 caccaaaact gctgatcgac aattcaccac ccaaggaaat atttctaaac agattctttc
    22861 gttagaaaca gaattagacg tcaaattatt cgaacgcgcc catagaaaaa tcgaattgac
    22921 cgaagctgga agcttattgc tcccatatgt aaaaaatgtc gtagaacaat accatgctat
    22981 gcaagaagtg cttgaaacct atacgaaaga caacaatctt tcattaaata tcttaacgat
    23041 accgacaatg atcaattata aaggtttttc caaaatcacc gagtttttga aacgccatcc
    23101 tgaatttaca gtccagttga aagaagtgga aagtgtcgaa ttaacttaca atgacgccaa
    23161 tgaacatagt gacacgatcc actttgctag aaatttccag gccccaccaa gttcgattga
    23221 gtggttgcct actgaaacag acgattttgt agcagttcta cctaaaaatc atcctttagc
    23281 tagtaaaaag aaattggatc taagcgaact gaaaaaggag aactttttgc tccttggccc
    23341 cactacgaac ttgtatcaac ctgtactaga tctgtgtcgc gaagctggat ttgaaccaaa
    23401 aatctcttat aaaggagcaa gaatcgactt gatcatcaat atgattgcaa atgacatggg
    23461 gattgctatt gttatggaaa aaacagtcaa gaatctatta aaagaaaata ctgtgattct
    23521 ccctatttct ccaacaacag agagttatct tgcatttact cgtaaagtcg gtgagcattc
    23581 tttggcttca gatacatttt gggagtattt aaatcaagaa gaaaactaag ccctacattt
    23641 tctgtccatc tatccaccca aaaaaagaga gcgacgcatg actttcgctc tctttcttgc
    23701 atcatgtcct tgtttcaatg acacctctaa catttctcgt agttcaataa gaaagcagcg
    23761 atttttatgc caaaagaacc gtaagaacat ttccctaatc acaaaaaaac gactaaaaac
    23821 ccctaaaaac cttcaataga atttttaggg gttttattct ttttcctatt gtttacttaa
    23881 ccgtatcatt gctgtcgttc ctactccttc gatactgcta taatcaatac gtccatcatg
    23941 cagttctaga atttttttaa ccagtgaaag tcctagccca ttcccagaaa tttgacgact
    24001 tgtatctcct tgataaaatt tttcaaacat tcgatctctg gtctcttcat tcattcctac
    24061 acctgaatcg gtcacttcca gtccaatctc tgtggctgtc tcaaatagtt tgatatgaat
    24121 ctgtccattg atttgattgt actttatggc gttatccatg atattcagcc acacttggta
    24181 caaaaactct tcattgcctg tgtaatttac tgcagcaagt tcgatatcca attctagctg
    24241 ttctttctcc catttaggct gcaaaaaaac gattacttct ctgatttgtt catctaatcg
    24301 atactctttc ttttccagtt ggatacgctg gttttccagt ttgttcaatt ttaagatgtt
    24361 ctctgttaat tgtgaaagct gcgtgatact ttcgataatc cgatataaaa atatttggcg
    24421 ttcttcgtca gagagatttg gggcttgtaa caattgaaca tatccttgga ttgttgctag
    24481 tggtgttttg aattcatggg atacactgga cacaaagtca ttcctcagtg tctcgatact
    24541 attgagttct tggaccataa cgttgaaatc tttatacagt tgctgtactt ctgctacttt
    24601 ctgctgttca tccattcgaa tcgaaaaatc tcccgttgct accagactca tatttgtccc
    24661 tagcgcactg attggatgca ggatacgccg cccaacaaaa atcgatattc ctgtacccac
    24721 gaccatactg aaccccgcaa agacgaataa cgggtataac gttggttttc cagccgattc
    24781 agcaggcgag atagaatgtc gagacaataa gaaaataaaa ccgagaaaac acagcagtgt
    24841 cactaagatc gaaatgaaga caatactagc aaaatagatc cataattgtg aaaatagctt
    24901 ttttttcatc tttaaataac cgccttatag cctagtcccc gtactgtgat gatttgaaaa
    24961 tcttccgtct tttcaaaacg ggtccttaaa cgtttgatat gtacatcgat cgttcgttct
    25021 tctgtattcg tatctaatcc ccaaatatca tccatcaact gttggcgagt gaagatcttg
    25081 ttgggataag acagtaattt atacagaagc agaaattctt tttgaggcag aatcaacgaa
    25141 tgctgatctt gtaacactgt ataagcatct agctctaatt ttgtttgtcc gattatcaag
    25201 ctactgctgt aattgatctt tgaacgtcga agcaatgctt ccacacgtag gatcatctca
    25261 ttgacatcga tgggttttgt catataatca tctgctccta atttgaaccc gcgtttctta
    25321 tcctcaaaat cggcctttgc cgtaatcatt agaacaggtg tgtcaaatct ggtgctacgt
    25381 aatgagtcta gcaagtcata gccatccatt ttcggcatca tgatatctgt aatgattaat
    25441 tctatacgtg tcgcttctag ttttttcagc gcatcctctc cattcaatgc ggtgatgact
    25501 gaaaagcccg ctcgttccaa cacagaacgg taaagcaact gtaattgcgg atcatcttct
    25561 actactaaaa tacgattcat tgatctccct ctgttttttg aaaaatttct agattttttc
    25621 ttgtagttca tcttcagtta acatttattt cgtatactac gatcattgtt ctgatatact
    25681 tattagtata ccgaatattt caatacggga gaagggtgat tatgcttcaa ttaaaagata
    25741 taaaaaaata ttataaagtc ggcgaaacca ccaccaaagc attggatggt gtgtctgtcg
    25801 ctttcagaca aaaagaattc gtagcgattt taggtcctag tggttctggt aaaacgacta
    25861 tgctgaatgt gattggtgga ctagataatt atgattccgg tgatatggta atcaacggaa
    25921 agtctactaa agactttaag gacagtgact gggatgccta ccgaaacaac tctatcggtt
    25981 tcatttttca aagttacaac ttgattggtc atctaggcat tattgaaaac gttgaacttg
    26041 ggatgacatt aagtggtgtt tctaaagacg aaaaacgaaa acgggcagaa gatgcattgc
    26101 accgagtcgg cttgacagat catatgcata aaaagccaaa tcagttatct ggtggacaaa
    26161 tgcagcgagt ggccatcgct cgagcactag ccaatgaccc agatatcctt ttgtgcgatg
    26221 aaccaaccgg agcgttagat acagaaacaa gtatccagat catggaattg atccaagagt
    26281 tatcgaaaga aaaattagtg atcatggtca cacataatcc tgaactggcc catcaatacg
    26341 cagatcggat cattgaattt tctgatggta agatcttaac agattcccat cctcacatcg
    26401 aacgaccaaa agatgatcaa tttaacttac gcagaacaaa gatgagtttt tggacagcgt
    26461 taaagctttc cttcaacaat atccgaacaa aaaaaggacg tactttcttg acctcctttg
    26521 cttccagtat tgggatcatc ggaatcgcga tcgttctgtc cctttcttct ggattccaaa
    26581 aacaaatcga taacacgcaa gctgaaacga tggcgaaatt tccgattact atttctaagg
    26641 taacgactaa ccagactcgt gacgatgccg gattgggtgc aagcaaagcg gattatccag
    26701 acagcaaaac gattactgca aaagtcagcg aagaagataa agcacagcac acgaataaga
    26761 ttgatcaaac atacgtcgat tacgtaacag acatcgatcc taacttaagt aataatatcg
    26821 gatttacccg tactacagga atcaatcttt tacgtgatgt aaatggcaaa gtacagcctg
    26881 tcagcttctc gaatcaaaat cctgatacgg aatctctttc tttatctagt acgatgtcag
    26941 caatgacagg tgtgggagtg tcaagtttcc ctacacagct tgacactagc aaagaaaact
    27001 ttttaaaaga caattattct ttacttgccg gaagttatcc agcctcagca acagatgtcg
    27061 tgctgatcgt agacggcaat aataatacta atatcaacgc attgaagaat cttggatttg
    27121 atgtgaaaga agacgaaaaa cttgattttg atgatatcgt tggtacaaca ttcaaattag
    27181 taaacaacaa tacttactat acaaaacttc caactgggaa ttttatccct aatacagatt
    27241 atgatgcgat gtatcagaat gcttctgatg aattgaagat ctcggggatc ctacgcgtga
    27301 aatcttcttc tacaatgaat ctcttatctc caggaatcgc ttatagcgat caattaacga
    27361 ctcaaatcgt caacgaaaac aaagaatcag aaatcgtcaa agctcaaaga gacagtggtg
    27421 taaatgtctt aactactgaa aaagttgacg aaaatgccaa acaaacattg cttagttatt
    27481 taggtggaga cagcttgcct tcaagtatca tgatttatcc gaacaatttt gaagacaaag
    27541 aaaagatctt ggattatcta gatgattaca ataaaggcaa atcagatgaa gataaaatca
    27601 tttatactga tttagctgga acgatgactg aactgactgg cggattgatg gatgctatca
    27661 cttatgtcct tattgctttt gcagggatct ctttagtaac aagtatgatc atgatcagta
    27721 tcattactta tacatctgtg attgaacgga caaaagaaat cggtgtacta aaagctttag
    27781 gtgcacgtaa aaaagacatc actcgcgtat ttgacgcaga aacgtgtatt ttaggtattt
    27841 cttctgggat attaggtgtg tttattgcat ggctagctac gttccctatc aatagcatct
    27901 tatacaacat gacggatcta aaaaatgttg ctcaactaaa tcctgtccat gcgattattc
    27961 tagtgatcgt ctctactata ttgacgatgc ttggcggtca tcttcctgca cgaatggcag
    28021 cgaaaaaaga cgccgctatc gctttgcgtg cagaataagg ttgtgacaac cacatttaga
    28081 tttgagtcat aagagcgctg aaatagtaga aaacagcaag aaacgaggaa gtaatatgtt
    28141 ccttgtttcg cgctgttttt tttaattatt ttttataatt ctggtatact tttctttcct
    28201 ttgtaccaca actcttctgg cgtatacctg tcttttccat ttatgaatac tgttccatct
    28261 gcttcaatcg ttacttcagt cccaatccac atattacgag aaagtgcgag aagatttttt
    28321 ctttttcctc ttcctttttt tccaaattgc aaggcatcgg ttatctcttc tgtccaatga
    28381 gatttcacgt attttacttc tccgttttca aatgtaactt tcaaaatatt cgctatattt
    28441 tccactttta ctgctttagg aaatgactcc ttcatttttt tcactttcct tcctccaaca
    28501 ctaagtatgc gctttctttt agtgataagt atatcatagt ttattctatt atcaaaaaaa
    28561 cgcatccatt ccattgtaga atactttacc gaacaagttg aattgttttc ctctttttca
    28621 ccttttacta tagataggta aagacaacta aggaggaatc tctcatgaca gatcaaccgt
    28681 atgatttaag aaaagtcatc gaagaactaa aaacacttcc aggtcaatat cacgaaacag
    28741 atattgaaat cgatcctgaa gcagaattat ctggtgttta ccgttatatc ggtgctggcg
    28801 gtacagtaaa acgtccgact caagaaggac cagctatgac tttcaacaat atcaaaggtt
    28861 tccccggcac acgtgtgaac atcggaacaa tggctagtcg taaacgtgta ggtgaaattt
    28921 tgcaccatga ttatcgaaca cttggtcgtt tcttgaaaga cgctgtagaa aatccaatca
    28981 aacctgtgaa agtagacaaa gcagacgctc cagcacaaga agtcgttcat ctagctaccg
    29041 atgcagactt cgatattcgc aacattttgc ctgcacctac aaatactcct gaagatgctg
    29101 gtccatacat cacaatgggt gtagtcttag gatcagatcc agaaaaaaca atgagcgatg
    29161 taacgatcca tcgtatggtt ctagaagata aagatacgat cggtatgtac atcatgcctg
    29221 gcggacgcaa cattggtcac ttccaaaaac aattcgaagc cttgaataaa ccaatgccta
    29281 tcactatcaa tattggttta gatccagcaa ttgccattgg cacgactttc gaaccaccta
    29341 ccactccact tggctacaat gaactatggt gtgcaggagc tttgcgtaac gaacctattc
    29401 aaatcgttga tggtatagca gtcgatgaag tgggtattgc tcgtgctgaa ttcatcattg
    29461 aagcagaaat catgccaaat gaaacaatgc aagaagatat caacacgaac tcaggaaaag
    29521 caatgccaga atttcctggc tatactgggg atgctaatcc agctgtacaa gtagtgaaag
    29581 tcaaagcagt tactcaccga aaagacaatc caatcatgca aacaacgatt ggtcccagtg
    29641 aagaacacgt atctatggcc ggtattccta cagaagcaag tattcttgcc ttggtcgata
    29701 aagctattcc aggaaaagta gtaaatgtct acaatcctcc tgcaggtggt ggtaaattga
    29761 tgaccatcat gcaaatccgt aaagaaaacg aagcggatga aggtattcag cgtcaagcag
    29821 ctattcttgc tttatcagcc ttcaaagaat tgaagacagt cattttagtc gatgaagatg
    29881 ttgatatttt cgacatgaac gatgtagttt ggactttcaa cactcgtttc caaggagata
    29941 aagatatcat cgtgatccca ggaatgagga accatccatt ggacccatca gaattaccag
    30001 aatataatcc tggctctatt cgtttccgtg ggatgagtgc aaaaactatc tttgacggta
    30061 cagttccttt cgatatgaaa gaacattttg tacgtgcaaa attccaagaa gtagaagatt
    30121 gggaaaaata tttgaaataa tgaatgatct gcaactgttt tgacatcata aaaaggagtg
    30181 cggaaaatgg aacaattggc acaacggaag aaaaaaatcg tgattggtgt aagtggcgct
    30241 tcaggcacca tttatgccat caacctagtg aagaaactaa aagaatatcc gatgatagaa
    30301 acacacggaa tcatcagtcc atgggcaaaa cagaacttaa agctggaatc cgacttatca
    30361 ctaactgaat tcgaaagcct gtttgattac cattattcaa acaaagattt aggtgctgct
    30421 gtcgccagcg gatcattttt gaccgatgga atgatcatcg taccggcaag tatgaaaaca
    30481 gtcgcaggta tctctgttgg tttaggcgac aacctgattg cacgtgctgc tgatgtaaca
    30541 atgaaggagc aacgaaagtt agtcattgtt cctcgtgaaa cgccattaaa tgccattcac
    30601 ttggaaaact tgactaaatt agcacgacta ggtgtgcaaa tcataccacc aattcctgct
    30661 ttttataatc agcctcaaac gatccaagat cttgttaatc accatacaat gaaacttttg
    30721 gatcaatttg gaattgtaaa tgagcattca aaacgttggc agggtgatta atatggaaaa
    30781 agaaacgata gttgaagtaa ccaaactagg tttcacgatc caagctgctg ttaaagtaat
    30841 cggtaaagac ttattgatca ctttaacagg cggagataca ccacatatcg gaacagtcac
    30901 tacattttct cgtgatacag agatgcaaac tgtacgtttc cctagccacg atggtcggtt
    30961 ccataaagac gatgtcttag ccgaaaaaat agctgtgatc atcaaaactg ccgttccggg
    31021 aagttgtacg atcacttccg gtatccatgt tgaccatatc tccaatgcac aaatcaacgc
    31081 ctcttttccg atgtcagaag agttaggtta caagatttta gattggttgg aaaaaataga
    31141 tttccatatc aaagaaccag tttattataa agatggtgaa aaaacattat gaaagaagag
    31201 gttgtgaaat cagcgttatg acctgagtat gagagaaaaa aatggaaaaa tagcttctca
    31261 tattttgccg tttttgagct aataccgagg gacagctgat tgaacgccgt ttatcaagag
    31321 gttgtgataa agccgcttcg tttcgagtag taagaaaaat tttgtaaaca tgggtttcca
    31381 catttttgca taaatttgac ttactaccga gaaactggct tttgaacacc gtttatcaag
    31441 aggttgtgac acgtcttttg tcacaacctc ttttattttt gcttcactgt aactgctaca
    31501 atatcaccga tttcttttcc gatgttttta cgaatatctt ttcgtagtcc aaggatgtag
    31561 cagactgaac catctggatt tttcacgccc atattcacga tactgccttc atacggcata
    31621 ttgtcaaaag ttgcttggac tttaactctt ccagcaccga attcttttct tatatcatac
    31681 ggaaaaataa tatatgcccc gcccttgtcg acttcggaag catagatttt tgattcaaaa
    31741 gaataaattt ccctcatttt atttctccta ttttttacat ctagtatagc atagcaaaaa
    31801 aaagaaggaa ctgtgacaaa agtcttgtcg cagttccttt tttcgaataa acggtgttct
    31861 cctgccaact cctcggtcac aagtcacaat agtcaacaaa catgagaagc tgttttttga
    31921 aattgctcct tgtgactcag agttaaacgg caggctcaca acctcttcct taaacggtgt
    31981 tcaaaagctt gcatctacgg cactaagccc aatcagatga aaaatatgga gtgctatttt
    32041 tatctgattg ttcttactgc ttgatgcaga gcagcttttt cacaacctcc tacatggtgt
    32101 tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg ttgttggaat
    32161 tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt tggattttca
    32221 gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc catcgtactt
    32281 tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa ttctacccat
    32341 gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt atcagcaata
    32401 ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt cgtcactttt
    32461 tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc agttgctttt
    32521 ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc attccctaaa
    32581 gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat tttttctgtt
    32641 tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac gatcaaactt
    32701 agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca tgttttttta
    32761 gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc aataatcatg
    32821 cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag gcaataataa
    32881 agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct gacagtgtgg
    32941 cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat ttgcttggga
    33001 tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca atcgagcttg
    33061 cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta tttggcaatg
    33121 cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac atacggatcg
    33181 cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca aatggtgcag
    33241 ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg tatttatcga
    33301 aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc aacaacatca
    33361 agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt acgaccattt
    33421 ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc gtgaatgtag
    33481 atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga cgccatgaga
    33541 acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag cttccccagc
    33601 cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc gttgataaaa
    33661 tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc attaggaaga
    33721 atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag tcacgccaat
    33781 ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca agtccaataa
    33841 cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata gatagcatga
    33901 tattttgcaa taatggcatc gatactccca cgccgcaagc ttggatcaag cgaccgatta
    33961 gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca
    34021 tggcagaaag atataaattt cttgaactga atttattgat cagccatgct gttattggga
    34081 tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca gacgctgaaa
    34141 tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg
    34201 tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat ggttttccgt
    34261 aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt tttctgaaac
    34321 tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac ataagttaat
    34381 acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt tggggcttcc
    34441 cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt aagtgatatc
    34501 gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat tcgttcgatc
    34561 gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa agtagaactg
    34621 atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc
    34681 cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa
    34741 ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga gaatgctaag
    34801 atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct tcctgtcgat
    34861 gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat ctcctaagta
    34921 ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg tttgaacata
    34981 agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc catcactgtt
    35041 cggatatttt tttatttttt tcggttactt tcttctaata catctgttag ccaatttgca
    35101 tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta attttcatgt
    35161 ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag tcgtttttct
    35221 tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt cacgtctttg
    35281 acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt ggttggtgta
    35341 tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac tttcttttcc
    35401 aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc catttttgca
    35461 agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc atttttgaac
    35521 gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa caaaatatat
    35581 cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta aatccattct
    35641 atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta taaggagatg
    35701 cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact ttatctatac
    35761 gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg ttgattaccg
    35821 tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg atatcgttaa
    35881 attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag acgtggcatt
    35941 ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta aatgggttga
    36001 agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa tggaagcagc
    36061 tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta cgatcactta
    36121 tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat acgatggaat
    36181 gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag taaaataagg
    36241 ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa agaaacggaa
    36301 aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
    36361 ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa cgctagttag
    36421 aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca gccgtttgaa
    36481 caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaatg
    36541 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
    36601 acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta gtgacttcaa
    36661 aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt attctgttca
    36721 tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta ctgcttcatc
    36781 tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag ctgattgatt
    36841 tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt tcattagttg
    36901 cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga taaactgatc
    36961 aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa tcactgcatc
    37021 tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg gaatgaattc
    37081 atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt ctttggcaga
    37141 caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga ttttaaaata
    37201 aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt atatcttctc
    37261 ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag ctgaaacgat
    37321 tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt cgctttgtac
    37381 tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa tgaatgaact
    37441 accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa gcactttttt
    37501 acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga ctacagctat
    37561 tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc cttttccttc
    37621 ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca aacgatgcgt
    37681 gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt ctgaaatcgt
    37741 tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat aagccaattt
    37801 ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta attcggtaaa
    37861 taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg aatttccacc
    37921 aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc taccattgag
    37981 ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc cgatcagcca
    38041 ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga aaggatcagt
    38101 accaggattg aatccttggt cagttaatcg ttgttttttc tcctccattt tctttcgctg
    38161 cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt gatcaataaa
    38221 actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta cgacgataca
    38281 tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac tctgcttcac
    38341 atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt caggaaaaaa
    38401 atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt aataagtaca
    38461 gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg cagatgcaag
    38521 cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg agtaataaga
    38581 gcgctagtca gaaaacagct tctcatattt tctaggctag tgagcttatt accgagaagc
    38641 agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt cgagtattaa
    38701 gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc ctctctcctt
    38761 agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa ttttctaatt
    38821 tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc aaaggagttc
    38881 gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg gcatttctca
    38941 tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc cattacaatc
    39001 ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt ccttcccttt
    39061 atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt ttcgcttatt
    39121 tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt ctttcaaatt
    39181 tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat gtcaatgttc
    39241 gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct attcaatact
    39301 ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta tgtatgatcc
    39361 ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct gtctcagtgg
    39421 gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg tatatccctt
    39481 tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt caactgatta
    39541 agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta gctattgttt
    39601 acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa aaggcgatag
    39661 atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg atattatcag
    39721 gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa atcgtgttgt
    39781 acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg acattgatcg
    39841 aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc gtgccattta
    39901 cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa caaaagtggt
    39961 tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc cagaacttat
    40021 accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact tatctattcg
    40081 atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc agcttgttcg
    40141 ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa gacaataaat
    40201 acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa acacatccag
    40261 gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat atccctgtta
    40321 tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat tatgaagtaa
    40381 cagatatcgg gaccccaatc gtgaagcaga aaaaaggaat caacgagctc acgcttcatt
    40441 atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc tggtttacct
    40501 tgctatgttt atttatttat catagataca agcttttacg tgcttaggca aaagggataa
    40561 atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt tattttaatc
    40621 agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg atttccaccc
    40681 cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga tattttttaa
    40741 cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta ttttgttccc
    40801 aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg acaacatacg
    40861 aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag tctactggat
    40921 tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag ttttccaagt
    40981 ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag tggcagctcg
    41041 agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg cgtgtctgat
    41101 aatcttcttt cttttgagat tgggagacgg taaggacctg actttccaat gtttttttcg
    41161 taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt tcccaactcc
    41221 aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca aaattatctt
    41281 tacggatagg tacttgattc tttacctgac agaacttcat tagcttcgca ggttctactt
    41341 cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa tcttctaaca
    41401 ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc cagctatgcg
    41461 tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa caaaagggtt
    41521 gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta actcctcagt
    41581 aataagctca atggaccagg agctattttt tgtttggctg tttttatcac ttggtgcagg
    41641 gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct cggtaataag
    41701 cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat gacttgatgc
    41761 aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact ttaccagctg
    41821 tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta aatacgatca
    41881 tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca gctaatttcg
    41941 ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc tgattcattt
    42001 ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt tccagcccaa
    42061 ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca acaacttctc
    42121 ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat accggatctt
    42181 ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct tgttctatac
    42241 ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct ggctctggaa
    42301 tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct gcttttggtc
    42361 cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct gctcgtttcc
    42421 aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac cgagtcatac
    42481 aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc tggaccactt
    42541 gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg tcattgtcgt
    42601 aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa tttgctaaga
    42661 aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga tctccaccta
    42721 gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca aatgaatgga
    42781 cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact gaataaatga
    42841 catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt acacctgtta
    42901 gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc ttatcaggat
    42961 cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa ccaatcattt
    43021 gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat tgcgacatat
    43081 cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac ggatcttgcc
    43141 caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg ccaagctgtg
    43201 tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt aaacgttcta
    43261 atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct gcaatccata
    43321 aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga atcaatgcca
    43381 cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg aaaaatgaca
    43441 aagcatccgg caattttcta taattatagt acttattata agccattgcc ccgacaaatc
    43501 cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat acacttgtaa
    43561 aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca tcttcattcg
    43621 caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt aaaataaatg
    43681 agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct cctattgcta
    43741 aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca attactccgc
    43801 ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc cctatactga
    43861 tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt ccaaattttt
    43921 gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg caatcggttt
    43981 cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt ttatttttct
    44041 gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt ttctcctaaa
    44101 aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg acttgtgcaa
    44161 tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa atgaaacaga
    44221 tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta gataaaaaaa
    44281 atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg atgcgcggga
    44341 actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg ggtgtctggt
    44401 atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac tttggaaaag
    44461 tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag ctgatcgact
    44521 tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat ggtgtattat
    44581 ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc gaacgttttc
    44641 tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg cttgaaggta
    44701 gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa gacagcaact
    44761 atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc ggctatttga
    44821 cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact gcagcaatgc
    44881 gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact ttagcattac
    44941 atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa aaagaaatta
    45001 tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct gtctcagaat
    45061 taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg gcttgggcag
    45121 aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa caaggtattc
    45181 gattcaattt gttccaacta ttctctactt actacggcga agatgaacga ttgaatatcg
    45241 gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat acagaagcgt
    45301 atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat ctgctgaaat
    45361 atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga ttaaaaggcg
    45421 cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg gaaatcacat
    45481 tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat acgaattaca
    45541 ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa atcgcacgct
    45601 tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc cacggtgtga
    45661 ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat acgatcgcag
    45721 cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa gctgctatca
    45781 ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg tacttcccag
    45841 ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa gatctcatgc
    45901 ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct tgggacaaaa
    45961 tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat ttcttcaatg
    46021 atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca atgactgtcc
    46081 atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta ggaatggaaa
    46141 gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat tacaataacg
    46201 acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg attgtccaag
    46261 gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt ctgccaaaaa
    46321 cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc atctcagtca
    46381 cagatgaaca agttactttc gagttattag aaggttttcc tctagaaatt tacgtctatg
    46441 aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag gaagcaaatg
    46501 tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga attccattat
    46561 cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac attcaacgaa
    46621 cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta tggcggaaaa
    46681 gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa cgattattat
    46741 ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt gcctttactg
    46801 gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa aaacgggcca
    46861 ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga cccggcagaa
    46921 gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc tgtcggacta
    46981 aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc catcctctca
    47041 agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga gatcccgctt
    47101 gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtgtca tgaccatgga
    47161 taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa tcacattgaa
    47221 gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc aaatcttcgc
    47281 accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt tcgaagatgt
    47341 tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa ggatccggga
    47401 tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata tccacggtgg
    47461 cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc catcatcaat
    47521 aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa tcactgctac
    47581 gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta gtagccttgt
    47641 tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa atggaacaag
    47701 agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat tagatcaaga
    47761 aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact ttagaaaatc
    47821 cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg tatttctttt
    47881 gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt ctggtcgcca
    47941 aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg gattcgaagc
    48001 tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa tcgaaccgca
    48061 agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc atcctggtca
    48121 agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga aaggtgtttt
    48181 tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt
    48241 ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga aagtccgaaa
    48301 aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag gaaaacgcat
    48361 ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga tcggtaaccc
    48421 attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg atatgactac
    48481 agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc gtatgcattt
    48541 acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagatc ctgtcattga
    48601 ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc atcatgagaa
    48661 cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa cagaattttt
    48721 gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg aaagtttgta
    48781 tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc
    48841 cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt tgaaagaaaa
    48901 cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga tgcagttggc
    48961 gcaaatgtac ggctatgtca ttccagagac actctccgtc attagcttca acaactcgat
    49021 cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct ccgaattagg
    49081 acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg
    49141 tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag aaaacccata
    49201 ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct ttttatgtaa
    49261 aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg acaaaagtaa
    49321 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    49381 cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt tcttcttatt
    49441 tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt ttttacacgt
    49501 cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat cgagaaaaag
    49561 acgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa tgtgccgctg
    49621 actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt tcgtgtcaaa
    49681 taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc tggaagaatg
    49741 atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa ggcagtaaac
    49801 ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat ggtatcaacg
    49861 atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa actagcagaa
    49921 cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg atcaggtgta
    49981 tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa acttttctca
    50041 tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg gatagccgga
    50101 atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca aggagagatc
    50161 caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc ggctgaatga
    50221 ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg gaaatccgta
    50281 taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc tgccaagttg
    50341 gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac gactgcgcct
    50401 gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc aattccaatc
    50461 gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca aacaagaaat
    50521 gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat aatcgtaggg
    50581 atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa aatcaggatt
    50641 ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc ccatgtgaac
    50701 tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg gatatgtggc
    50761 gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat caatacagcg
    50821 atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca aatgaaaagg
    50881 ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt
    50941 cggctgaaag acttattttt tttatttttc ttctaaactt ttttctacct caatccccca
    51001 agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca ctgtgatagc
    51061 tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat
    51121 gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt ttttaccaat
    51181 tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg aaacagcaat
    51241 caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca agaatacagc
    51301 acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga ttgattgtgt
    51361 tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga attttggtgg
    51421 aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa cccaagctga
    51481 taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga tcacttctgg
    51541 taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta attccatttt
    51601 tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata aaactaatcc
    51661 gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca aacgaggcat
    51721 gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta agtatttcaa
    51781 tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac ctagatagcc
    51841 gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca tgatgactac
    51901 tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca tatcgaattt
    51961 caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa cgaaccagtg
    52021 attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta aagtagaagc
    52081 gaccattacc atacctggga acatttgcac ccataatagc catgcaacga caaatcccca
    52141 tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag gaagcatcgt
    52201 ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg caaaaattga
    52261 ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa ccgttgcaca
    52321 cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt cagatttact
    52381 caccgttgac actctccttg attttttgga taaaataacg gtgtctttga agtagtttca
    52441 gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagtcta ttgcttgaag
    52501 ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct ccccttgctc
    52561 ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta acatgattac
    52621 cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc caatttttct
    52681 tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt gttcatgtat
    52741 ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca ttcttcatct
    52801 gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg gatagcaaag
    52861 tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta tgaagaataa
    52921 tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc tttttctttg
    52981 aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc gatttctttg
    53041 tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc accttcgatt
    53101 gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg gtgtgctgcc
    53161 caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc cccaagtaat
    53221 gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat aacgtctctc
    53281 atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc cattttatgt
    53341 gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg tacttcttct
    53401 aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct
    53461 tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta tccgccataa
    53521 gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca
    53581 acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac accgactaca
    53641 cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag ttcgttgatg
    53701 tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc gataccgatg
    53761 atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat
    53821 ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat tttttctggg
    53881 acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc ccaatcgctt
    53941 tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga tttgatgttg
    54001 cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat gtggccccag
    54061 ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc catttggctt
    54121 gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc aaaattgtac
    54181 gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg tccggcatta
    54241 tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac atcctttgtt
    54301 ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat aataggcatg
    54361 tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc attcaacaaa
    54421 gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt taggtttaga
    54481 tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta aattttgtcg
    54541 agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg atttttaatc
    54601 atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat taaaaaaaac
    54661 gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa ccagcgttct
    54721 attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt tcaaggtaaa
    54781 aacttactaa attctaatca attactttgg ccaaaaagta atttttctta ccttttcgaa
    54841 taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt aaatcggtaa
    54901 cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct tgacgtttag
    54961 atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt
    55021 caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt tctttaatat
    55081 ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct tcactatgca
    55141 caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca ggttctgtct
    55201 ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag aatttcaagt
    55261 atttcactac atcgcgatca tcttgattca accagaattg gtagaactca aatggtgaag
    55321 ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc gttccgtcag
    55381 ccttcagcat caatgggatc gttaaaccga acgcttctac ttctggtcct tctaattttc
    55441 ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg atttgcaatt
    55501 gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcagaatt tggtaagtaa
    55561 actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt gctaacatcg
    55621 tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag atttttgaca
    55681 accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat tttctcattt
    55741 gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac acacgttctg
    55801 ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga atatatggat
    55861 gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt ccgatatgca
    55921 tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct tcaacgagct
    55981 cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt aattcatcaa
    56041 taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac ctgctagcat
    56101 gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct tggcatataa
    56161 gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc caacatacct
    56221 ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa atgtaattcg
    56281 ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg gctttaacaa
    56341 ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata gagcgcaact
    56401 ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt cttttttgaa
    56461 tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa ctttttctgt
    56521 attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt tcttcgttac
    56581 aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa
    56641 gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa ttctaagttt
    56701 gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac
    56761 agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag caaaaaatct
    56821 ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata gtaagaacgg
    56881 atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac aactgatcga
    56941 ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg tggacccgca
    57001 aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga ctgttgaaga
    57061 attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta atttaaatga
    57121 tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc tacctgaagg
    57181 aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata tggataattc
    57241 cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga
    57301 gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg cctttgtcaa
    57361 gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga caggttacaa
    57421 cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa
    57481 agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct ggagctttgc
    57541 ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg taaaaaaaga
    57601 caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata ttctgattta
    57661 tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc aaaaatttgc
    57721 ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta caccagcttg
    57781 gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg gcattcgttt
    57841 tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg tccatgatgt
    57901 ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac caaaaaagcc
    57961 gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct tcggtgcgat
    58021 tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg cgtatatctc
    58081 cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact catccgaata
    58141 tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca tgatactgaa
    58201 actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt tttttgtata
    58261 gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac ggattttgtt
    58321 ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca acgctttcgc
    58381 ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc cttccttgct
    58441 gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta ccttatccat
    58501 tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc
    58561 tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct cccgtaatt
//
LOCUS       DAZAOG010000003        58189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000003 DAZAOG010000000
VERSION     DAZAOG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.003"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..922
                     /locus_tag="ACHDLD_000135"
     CDS             41..922
                     /locus_tag="ACHDLD_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3361901.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="ACHDLD_000136"
     CDS             935..2545
                     /locus_tag="ACHDLD_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3361902.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="ACHDLD_000137"
     CDS             complement(2622..3281)
                     /locus_tag="ACHDLD_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3361903.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="ACHDLD_000138"
     CDS             complement(3564..4229)
                     /locus_tag="ACHDLD_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN3361904.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="ACHDLD_000139"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="ACHDLD_000139"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN3361905.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="ACHDLD_000140"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="ACHDLD_000140"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN3361906.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6389)
                     /gene="dhaM"
                     /locus_tag="ACHDLD_000141"
     CDS             complement(5994..6389)
                     /gene="dhaM"
                     /locus_tag="ACHDLD_000141"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN3361907.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="ACHDLD_000142"
     CDS             6543..6863
                     /locus_tag="ACHDLD_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN3361908.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7832)
                     /locus_tag="ACHDLD_000143"
     CDS             complement(6975..7832)
                     /locus_tag="ACHDLD_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3361909.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7940..9243)
                     /locus_tag="ACHDLD_000144"
                     /pseudo
     CDS             complement(7940..9243)
                     /locus_tag="ACHDLD_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9240..9749)
                     /locus_tag="ACHDLD_000145"
     CDS             complement(9240..9749)
                     /locus_tag="ACHDLD_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361910.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9924..12059)
                     /locus_tag="ACHDLD_000146"
     CDS             complement(9924..12059)
                     /locus_tag="ACHDLD_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN3361911.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12142..13797)
                     /locus_tag="ACHDLD_000147"
     CDS             complement(12142..13797)
                     /locus_tag="ACHDLD_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3361912.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13799..14113)
                     /locus_tag="ACHDLD_000148"
     CDS             complement(13799..14113)
                     /locus_tag="ACHDLD_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3361913.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14283..14543
                     /locus_tag="ACHDLD_000149"
     CDS             14283..14543
                     /locus_tag="ACHDLD_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN3361914.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14557..14871
                     /locus_tag="ACHDLD_000150"
     CDS             14557..14871
                     /locus_tag="ACHDLD_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN3361915.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15601..16155)
                     /locus_tag="ACHDLD_000151"
     CDS             complement(15601..16155)
                     /locus_tag="ACHDLD_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN3361916.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16281..18209)
                     /locus_tag="ACHDLD_000152"
     CDS             complement(16281..18209)
                     /locus_tag="ACHDLD_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN3361917.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18515..19231)
                     /locus_tag="ACHDLD_000153"
     CDS             complement(18515..19231)
                     /locus_tag="ACHDLD_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN3361918.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19232..21055)
                     /gene="glpO"
                     /locus_tag="ACHDLD_000154"
     CDS             complement(19232..21055)
                     /gene="glpO"
                     /locus_tag="ACHDLD_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN3361919.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21069..22565)
                     /gene="glpK"
                     /locus_tag="ACHDLD_000155"
     CDS             complement(21069..22565)
                     /gene="glpK"
                     /locus_tag="ACHDLD_000155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN3361920.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22701..24161)
                     /locus_tag="ACHDLD_000156"
     CDS             complement(22701..24161)
                     /locus_tag="ACHDLD_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3361921.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24151..25446)
                     /locus_tag="ACHDLD_000157"
     CDS             complement(24151..25446)
                     /locus_tag="ACHDLD_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3361922.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25778..26455)
                     /locus_tag="ACHDLD_000158"
     CDS             complement(25778..26455)
                     /locus_tag="ACHDLD_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN3361923.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26485..27576)
                     /locus_tag="ACHDLD_000159"
     CDS             complement(26485..27576)
                     /locus_tag="ACHDLD_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN3361924.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27677..28111)
                     /locus_tag="ACHDLD_000160"
     CDS             complement(27677..28111)
                     /locus_tag="ACHDLD_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN3361925.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28695..30152)
                     /locus_tag="ACHDLD_000161"
     CDS             complement(28695..30152)
                     /locus_tag="ACHDLD_000161"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3361926.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30149..32044)
                     /locus_tag="ACHDLD_000162"
     CDS             complement(30149..32044)
                     /locus_tag="ACHDLD_000162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3361927.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32102..33877)
                     /locus_tag="ACHDLD_000163"
     CDS             complement(32102..33877)
                     /locus_tag="ACHDLD_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN3361928.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33927..35423)
                     /locus_tag="ACHDLD_000164"
     CDS             complement(33927..35423)
                     /locus_tag="ACHDLD_000164"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3361929.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35617..37239)
                     /locus_tag="ACHDLD_000165"
     CDS             complement(35617..37239)
                     /locus_tag="ACHDLD_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN3361930.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37688..39001)
                     /locus_tag="ACHDLD_000166"
     CDS             complement(37688..39001)
                     /locus_tag="ACHDLD_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN3361931.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39246..39470)
                     /locus_tag="ACHDLD_000167"
     CDS             complement(39246..39470)
                     /locus_tag="ACHDLD_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361932.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39490..39912)
                     /locus_tag="ACHDLD_000168"
     CDS             complement(39490..39912)
                     /locus_tag="ACHDLD_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3361933.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40056..40766)
                     /locus_tag="ACHDLD_000169"
     CDS             complement(40056..40766)
                     /locus_tag="ACHDLD_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN3361934.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40784..41281)
                     /locus_tag="ACHDLD_000170"
     CDS             complement(40784..41281)
                     /locus_tag="ACHDLD_000170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3361935.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41318..41770)
                     /locus_tag="ACHDLD_000171"
     CDS             complement(41318..41770)
                     /locus_tag="ACHDLD_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3361936.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41905..42510)
                     /locus_tag="ACHDLD_000172"
     CDS             complement(41905..42510)
                     /locus_tag="ACHDLD_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN3361937.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42960..43580)
                     /locus_tag="ACHDLD_000173"
     CDS             complement(42960..43580)
                     /locus_tag="ACHDLD_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3361938.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43582..44451)
                     /locus_tag="ACHDLD_000174"
     CDS             complement(43582..44451)
                     /locus_tag="ACHDLD_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN3361939.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44474..44881)
                     /locus_tag="ACHDLD_000175"
     CDS             complement(44474..44881)
                     /locus_tag="ACHDLD_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3361940.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44900..46189)
                     /locus_tag="ACHDLD_000176"
     CDS             complement(44900..46189)
                     /locus_tag="ACHDLD_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3361941.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46216..46518)
                     /locus_tag="ACHDLD_000177"
     CDS             complement(46216..46518)
                     /locus_tag="ACHDLD_000177"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3361942.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46543..48636)
                     /locus_tag="ACHDLD_000178"
     CDS             complement(46543..48636)
                     /locus_tag="ACHDLD_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3361943.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49125..49889)
                     /locus_tag="ACHDLD_000179"
     CDS             complement(49125..49889)
                     /locus_tag="ACHDLD_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN3361944.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50000..50869)
                     /gene="mdcB"
                     /locus_tag="ACHDLD_000180"
     CDS             complement(50000..50869)
                     /gene="mdcB"
                     /locus_tag="ACHDLD_000180"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN3361945.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50894..51970)
                     /locus_tag="ACHDLD_000181"
     CDS             complement(50894..51970)
                     /locus_tag="ACHDLD_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3361946.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51994..52587)
                     /locus_tag="ACHDLD_000182"
     CDS             complement(51994..52587)
                     /locus_tag="ACHDLD_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN3361947.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52591..54267)
                     /gene="mdcD"
                     /locus_tag="ACHDLD_000183"
     CDS             complement(52591..54267)
                     /gene="mdcD"
                     /locus_tag="ACHDLD_000183"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN3361948.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54257..54556)
                     /locus_tag="ACHDLD_000184"
     CDS             complement(54257..54556)
                     /locus_tag="ACHDLD_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN3361949.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54658..56304)
                     /gene="mdcA"
                     /locus_tag="ACHDLD_000185"
     CDS             complement(54658..56304)
                     /gene="mdcA"
                     /locus_tag="ACHDLD_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFN3361950.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56311..57222)
                     /locus_tag="ACHDLD_000186"
     CDS             complement(56311..57222)
                     /locus_tag="ACHDLD_000186"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN3361951.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57342..>58189
                     /locus_tag="ACHDLD_000187"
     CDS             57342..>58189
                     /locus_tag="ACHDLD_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3361952.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFY"
BASE COUNT        16323 a        12430 c        10027 g        19409 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 agtcgtagaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcagattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggtc gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatgggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatccgct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaagatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatagttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8341 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttcttt
     8401 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8461 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8521 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8581 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8641 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8701 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8761 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8821 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     8881 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     8941 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9001 actcgattgt atcttcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9061 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9121 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9181 aaaatacata atagacaaac aagtatctaa gaataaatta cctgtatggg tgattgagat
     9241 catttcagtt ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt atctagattt
     9301 ttctcttcgg agacagaata aaaacggacc gtttctctct cttcttttgt tccaaaacgt
     9361 tccgtataca cttcttcaag taattcttgt ctttttttca atttttcatc ttcatattca
     9421 aaagatttat ttactttttt gtatagattt cgattgttcc acctagtcaa tacaaatgag
     9481 gtaatcagga aactgaaaaa aatcaataca aaaaagccga ttaagaaata cagcattttc
     9541 ttttctgata aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg
     9601 aaaaagaccc atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt
     9661 tgccaaatct ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat
     9721 gcaacttctt ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt
     9781 aggagtttac catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga
     9841 aaaagaacaa aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt
     9901 tctttttatt ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt
     9961 cctctagata tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca
    10021 aatggatata agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt
    10081 tggcagtttt ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc
    10141 tcttctgttt gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct
    10201 agtattttca acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc
    10261 ggtcggatcg atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct
    10321 gtgattgctc cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg
    10381 tagtcgtcga aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga
    10441 ttctcgtcac ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat
    10501 tgtgccaggg tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt
    10561 accttttcaa caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt
    10621 gtgggttgcc aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga
    10681 tgctcatcca cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt
    10741 aaaagtttcc ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg
    10801 aataattcca actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt
    10861 tttgcttgtt tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga
    10921 agcctatcga tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt
    10981 ttagaaagga ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa
    11041 aacaaaggat ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc
    11101 tgctccgata cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc
    11161 tctgctgaaa gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct
    11221 aatgatggta ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc
    11281 aagtcatcgg atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg
    11341 atagcagagg ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca
    11401 tcccgaatga ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg
    11461 gtctcatctt ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca
    11521 aaaaacttga ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta
    11581 tcgattttat tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt
    11641 tcggcaattg gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt
    11701 gctgtaccga tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt
    11761 ccaaagtagg gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc
    11821 tgtttgatat tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc
    11881 aggttatcta agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac
    11941 tggtttcctt ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt
    12001 agcttttgat acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata
    12061 cacattctcc tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga
    12121 atgcaagctt tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt
    12181 ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat
    12241 tctttccgct agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct
    12301 gtctaattca tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa
    12361 tttgcccttt tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag
    12421 ttctgtcggc aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc
    12481 tgcatatcct agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa
    12541 ttctaattcc ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc
    12601 gatccgttta tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc
    12661 tggatggaag atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc
    12721 ttttcctgat acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact
    12781 taaaccggtg gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc
    12841 ttgatttctt cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc
    12901 tggtgaagca agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc
    12961 atcccccacc gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc
    13021 cccgcgtatg cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc
    13081 aatggataaa ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt
    13141 cccgtggtct tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga
    13201 caattctttt tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc
    13261 ttgttcgatc accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc
    13321 gatgaatcct gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac
    13381 tttatcgata tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg
    13441 aatcgatgga acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat
    13501 gtgtttttga ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac
    13561 ttcatgattt ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac
    13621 aagaagattt tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc
    13681 aaaagaaaca tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg
    13741 aagccgtgta gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt
    13801 atttccctcc tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat
    13861 cataaccaga tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa
    13921 tcacatagac ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat
    13981 ttttagctcc ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg
    14041 ctggattttc caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa
    14101 atagcaaatt cattttctgc acctcattta catttttata aatacctcat ggggtatatt
    14161 aaacaggagc tttattaata tgtcaaccag aatgactctt catttttttc actacttttt
    14221 atatttgcat aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg
    14281 ttatggagat tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc
    14341 gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa
    14401 gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa
    14461 attgctttga gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga
    14521 tcaagatgat tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat
    14581 gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca
    14641 gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc
    14701 aatgcagaat tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt
    14761 gctaatcata tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc
    14821 cttgatttag ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag
    14881 gttgcgacag aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc
    14941 tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc
    15001 caaacaaaaa tagcttctca tatttttcgt ttggttgggc ttattaccgc agatgcaagc
    15061 ttttgaacac cgtttaagga agaggttgtg aaatcagcgg tttgacctaa gtattagaga
    15121 aaaaaacggg aaaatagctc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
    15181 ctgattgaac accgtttaag gaagagattg tgaaaaagtc gcttagtttc gagtattaag
    15241 aaaaattttg tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa
    15301 ctggcttttg aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt
    15361 agagaaacaa acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag
    15421 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
    15481 caagaaataa tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga
    15541 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
    15601 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
    15661 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
    15721 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
    15781 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
    15841 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
    15901 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
    15961 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
    16021 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
    16081 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
    16141 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
    16201 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat
    16261 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
    16321 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
    16381 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
    16441 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
    16501 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
    16561 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
    16621 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
    16681 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
    16741 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
    16801 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
    16861 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
    16921 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
    16981 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
    17041 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
    17101 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
    17161 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
    17221 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
    17281 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
    17341 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
    17401 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
    17461 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
    17521 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
    17581 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
    17641 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
    17701 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
    17761 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
    17821 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
    17881 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
    17941 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
    18001 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
    18061 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
    18121 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
    18181 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
    18241 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
    18301 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
    18361 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
    18421 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
    18481 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
    18541 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
    18601 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
    18661 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
    18721 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
    18781 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
    18841 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
    18901 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
    18961 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
    19021 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
    19081 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
    19141 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
    19201 ctcccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
    19261 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
    19321 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
    19381 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
    19441 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
    19501 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
    19561 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
    19621 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    19681 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    19741 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    19801 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    19861 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    19921 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    19981 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    20041 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    20101 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    20161 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    20221 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    20281 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    20341 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    20401 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    20461 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    20521 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    20581 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    20641 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    20701 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    20761 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    20821 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    20881 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    20941 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    21001 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    21061 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    21121 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    21181 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    21241 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    21301 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    21361 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    21421 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    21481 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    21541 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    21601 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    21661 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    21721 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    21781 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    21841 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    21901 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    21961 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    22021 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    22081 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    22141 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    22201 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    22261 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    22321 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    22381 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    22441 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    22501 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    22561 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    22621 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    22681 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    22741 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    22801 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    22861 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    22921 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    22981 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    23041 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    23101 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    23161 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    23221 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    23281 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    23341 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    23401 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    23461 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    23521 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    23581 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    23641 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    23701 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    23761 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    23821 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    23881 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    23941 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    24001 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    24061 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    24121 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    24181 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    24241 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
    24301 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    24361 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    24421 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    24481 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    24541 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    24601 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    24661 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    24721 ttcccacatc tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc
    24781 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    24841 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    24901 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    24961 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    25021 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    25081 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    25141 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    25201 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    25261 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    25321 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    25381 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    25441 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    25501 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    25561 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    25621 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    25681 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    25741 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    25801 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    25861 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    25921 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    25981 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    26041 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    26101 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    26161 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    26221 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    26281 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    26341 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    26401 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    26461 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    26521 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    26581 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    26641 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    26701 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    26761 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    26821 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    26881 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    26941 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    27001 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    27061 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    27121 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    27181 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    27241 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    27301 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    27361 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    27421 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    27481 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    27541 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    27601 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    27661 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    27721 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    27781 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    27841 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    27901 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    27961 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    28021 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    28081 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    28141 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    28201 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    28261 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    28321 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    28381 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    28441 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    28501 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    28561 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    28621 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    28681 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    28741 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    28801 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    28861 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    28921 tgctgtttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    28981 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    29041 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    29101 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    29161 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    29221 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    29281 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    29341 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    29401 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    29461 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    29521 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    29581 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    29641 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    29701 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    29761 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    29821 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    29881 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    29941 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    30001 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    30061 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    30121 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    30181 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    30241 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    30301 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    30361 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    30421 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt
    30481 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    30541 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    30601 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    30661 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    30721 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    30781 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    30841 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    30901 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    30961 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    31021 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    31081 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    31141 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa
    31201 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    31261 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    31321 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    31381 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    31441 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    31501 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    31561 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    31621 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    31681 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    31741 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    31801 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    31861 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    31921 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    31981 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    32041 ccattcaatt agtacccctt ataatagcga acgaaactgt acaatagtgt gtatggttta
    32101 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    32161 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    32221 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    32281 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    32341 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    32401 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    32461 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    32521 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    32581 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    32641 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    32701 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    32761 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    32821 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    32881 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    32941 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    33001 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    33061 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    33121 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    33181 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    33241 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    33301 ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    33361 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    33421 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    33481 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    33541 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    33601 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    33661 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    33721 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    33781 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    33841 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    33901 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    33961 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    34021 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    34081 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    34141 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    34201 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    34261 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    34321 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    34381 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    34441 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
    34501 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
    34561 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    34621 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    34681 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    34741 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
    34801 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    34861 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    34921 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    34981 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    35041 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    35101 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    35161 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    35221 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    35281 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    35341 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    35401 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    35461 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    35521 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    35581 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    35641 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    35701 aataaccctt aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca
    35761 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    35821 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    35881 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    35941 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    36001 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc
    36061 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    36121 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    36181 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    36241 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    36301 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    36361 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    36421 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    36481 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    36541 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    36601 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
    36661 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    36721 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    36781 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    36841 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    36901 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    36961 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    37021 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    37081 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    37141 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    37201 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    37261 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    37321 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    37381 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
    37441 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
    37501 tatgaggaac tattttttcg tttttttctc taatactcag gccagaacac tgatttcaca
    37561 acctctttat aaacggtgtt cactcagctg tccctcggta ttagctcaaa aaacggcaaa
    37621 atataagaaa ctattttttc gtttttttct ctaatactca ggccagaacg ctgatttcac
    37681 aacctcttta tttcacattc aatatccaca tgaatcccac aaatacaaag aacagaccat
    37741 acatgattgg agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga
    37801 taaaacctaa tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca
    37861 agaatgaagg gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca
    37921 tcaatactcc taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta
    37981 atggagaaaa gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg
    38041 aacgtcctcc aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac
    38101 caaataagga acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag
    38161 gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg
    38221 tatcaaagaa agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga
    38281 tgtcccctac atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa
    38341 caatttcaga aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcta
    38401 tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc
    38461 caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt
    38521 cattcgaaac gatgatccca ccttcgctca gaccaaggaa agcgataaat agaccgatac
    38581 cgccagaaat cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct
    38641 tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt
    38701 cccatggaat ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag
    38761 caggtgccgt tccaattgga taacgggcta atacccccat taaaacacag ccgatcgcac
    38821 tggctaatgc agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag
    38881 ttggatttac aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct
    38941 cacgttttac cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca
    39001 tcttccatcc ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta
    39061 ataaaacaac gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat
    39121 tttaagcaaa atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca
    39181 ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc
    39241 aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct
    39301 tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc
    39361 cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt
    39421 tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac tgtttttcct
    39481 cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc
    39541 agaaaaattg aaagtcaaga tgccattgtt atctgtgatt tctcctggtt cttccatgtt
    39601 ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac
    39661 ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga
    39721 gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc
    39781 accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac
    39841 acgtgttttt tgtatcatca tttgaccctt ctttctattt gattttcttt cctgtctata
    39901 aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa
    39961 ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaaaa
    40021 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    40081 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    40141 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    40201 accgctgctt gtgtttcttc cgtcagcatt gttctttggg cttcacgcca gttgagacag
    40261 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    40321 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    40381 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    40441 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    40501 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    40561 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    40621 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    40681 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    40741 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    40801 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    40861 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    40921 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    40981 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    41041 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    41101 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    41161 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    41221 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    41281 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    41341 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    41401 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    41461 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    41521 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    41581 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    41641 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    41701 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    41761 tttcagccat ttcatcccac ctttattttg tttgtaagac tagtttacct ctttttgttg
    41821 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    41881 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    41941 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    42001 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    42061 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    42121 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    42181 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    42241 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    42301 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    42361 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    42421 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    42481 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    42541 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    42601 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    42661 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    42721 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    42781 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    42841 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    42901 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    42961 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    43021 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    43081 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    43141 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    43201 cgatcacgtt tgtaccgacg ttgacataac acttcaaagt ctgctgttag gcgaatcgtg
    43261 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    43321 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    43381 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    43441 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    43501 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    43561 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    43621 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    43681 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    43741 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    43801 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    43861 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    43921 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    43981 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    44041 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    44101 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    44161 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    44221 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    44281 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    44341 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    44401 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    44461 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    44521 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    44581 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    44641 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    44701 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    44761 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    44821 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    44881 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    44941 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgatgaat cccatcgaat
    45001 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    45061 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    45121 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    45181 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    45241 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    45301 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    45361 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    45421 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    45481 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    45541 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    45601 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    45661 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    45721 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    45781 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    45841 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    45901 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    45961 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    46021 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    46081 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    46141 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct
    46201 cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc
    46261 aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc
    46321 gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga
    46381 aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac
    46441 atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa
    46501 aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga
    46561 aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc
    46621 ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac
    46681 tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg
    46741 ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg
    46801 ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat
    46861 catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag
    46921 ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa
    46981 aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa
    47041 taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac
    47101 atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag
    47161 ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg
    47221 atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt
    47281 gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat
    47341 ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt
    47401 acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt
    47461 cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc
    47521 ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat
    47581 gttttctttc aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc
    47641 catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt
    47701 ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat
    47761 tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc
    47821 tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa
    47881 gctactgtct tttatttctg tatttttcca attaatggca gagataaatt catgtttcct
    47941 gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc
    48001 ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa
    48061 ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc
    48121 ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga
    48181 acctttataa accagtcctt gctttccata actgacgatc tctatcccat aactgaccag
    48241 tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc
    48301 ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa
    48361 aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg
    48421 agcttgcttt tccttttcca gcttcaacaa aaaaactttg ttgcgaattg tacggatctc
    48481 cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg
    48541 ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat
    48601 gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca
    48661 agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga
    48721 aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga
    48781 catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc
    48841 tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg
    48901 gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca
    48961 gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca
    49021 aaatcgtgcc ttattgccga taggctatct ctggcacact gtttattcag aaaaaggagc
    49081 tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc
    49141 ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc
    49201 tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg
    49261 actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt
    49321 cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct
    49381 ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg
    49441 gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt
    49501 tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct
    49561 ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact
    49621 gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga
    49681 ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat
    49741 gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga
    49801 ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa
    49861 atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa
    49921 ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt
    49981 tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag
    50041 tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc
    50101 caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta
    50161 aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac
    50221 tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt
    50281 gtttctgtac atatgaatca ttatctagct ccttagctgc ttggattgca agcctgatat
    50341 gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc
    50401 tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc
    50461 tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa
    50521 tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg
    50581 tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact
    50641 cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg
    50701 tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg
    50761 catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca
    50821 acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc
    50881 cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta
    50941 accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc
    51001 ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata
    51061 cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg
    51121 gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac
    51181 tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat
    51241 gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat
    51301 aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat
    51361 aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag
    51421 tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca
    51481 atactggcca tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg
    51541 gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac
    51601 ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg
    51661 ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa
    51721 acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa
    51781 caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa
    51841 aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca
    51901 taacagccgt tacagctgat actttctgga agtcacttac gttgattttg atttttttgt
    51961 tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg
    52021 tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga
    52081 aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga
    52141 ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc
    52201 actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat
    52261 tccccattta catcctgata atatgtttct agcagcatga aattgctgat aaacaggata
    52321 atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac
    52381 ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg
    52441 taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat
    52501 cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa
    52561 aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc
    52621 acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt
    52681 tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat
    52741 gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata
    52801 gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc
    52861 agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat
    52921 cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg
    52981 tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc
    53041 ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa
    53101 ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt
    53161 cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc
    53221 ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac
    53281 gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact
    53341 aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact
    53401 tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg
    53461 gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc
    53521 aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat
    53581 tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat
    53641 ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga
    53701 atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct
    53761 cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa
    53821 tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat
    53881 tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc
    53941 acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac
    54001 atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa
    54061 ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac
    54121 gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg
    54181 atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc
    54241 gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta
    54301 ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat
    54361 aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg
    54421 tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc
    54481 ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat
    54541 aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt
    54601 attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta
    54661 tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc
    54721 atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc
    54781 ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt
    54841 cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt
    54901 ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct
    54961 ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa
    55021 ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa
    55081 tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga
    55141 atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg
    55201 tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa
    55261 catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc
    55321 gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc
    55381 gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg
    55441 atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata
    55501 agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg
    55561 tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc
    55621 tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt
    55681 cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc
    55741 gttgacttga acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt
    55801 ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac
    55861 taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg
    55921 gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc
    55981 accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc
    56041 tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg catctacttt
    56101 ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt
    56161 gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt
    56221 gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc
    56281 ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc
    56341 gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt
    56401 tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc
    56461 taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa
    56521 caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata
    56581 caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt
    56641 atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc
    56701 ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt
    56761 atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt
    56821 tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc
    56881 gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc
    56941 aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc
    57001 aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag
    57061 taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata
    57121 ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa
    57181 cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc
    57241 acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa
    57301 gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc
    57361 agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta
    57421 tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt
    57481 tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat
    57541 atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg
    57601 agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact
    57661 tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt
    57721 taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga
    57781 ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg
    57841 ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa
    57901 agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga
    57961 cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag
    58021 ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc
    58081 gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag
    58141 atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattt
//
LOCUS       DAZAOG010000004        53431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000004 DAZAOG010000000
VERSION     DAZAOG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.004"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..978
                     /locus_tag="ACHDLD_000188"
     CDS             121..978
                     /locus_tag="ACHDLD_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN3361953.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1093..2013
                     /gene="pstC"
                     /locus_tag="ACHDLD_000189"
     CDS             1093..2013
                     /gene="pstC"
                     /locus_tag="ACHDLD_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN3361954.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2013..2897
                     /gene="pstA"
                     /locus_tag="ACHDLD_000190"
     CDS             2013..2897
                     /gene="pstA"
                     /locus_tag="ACHDLD_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN3361955.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2912..3718
                     /gene="pstB"
                     /locus_tag="ACHDLD_000191"
     CDS             2912..3718
                     /gene="pstB"
                     /locus_tag="ACHDLD_000191"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN3361956.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3734..4492
                     /gene="pstB"
                     /locus_tag="ACHDLD_000192"
     CDS             3734..4492
                     /gene="pstB"
                     /locus_tag="ACHDLD_000192"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN3361957.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4505..5182
                     /gene="phoU"
                     /locus_tag="ACHDLD_000193"
     CDS             4505..5182
                     /gene="phoU"
                     /locus_tag="ACHDLD_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN3361958.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5759..6451
                     /locus_tag="ACHDLD_000194"
     CDS             5759..6451
                     /locus_tag="ACHDLD_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN3361959.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6448..7215
                     /locus_tag="ACHDLD_000195"
     CDS             6448..7215
                     /locus_tag="ACHDLD_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN3361960.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7315..8883
                     /gene="liaX"
                     /locus_tag="ACHDLD_000196"
     CDS             7315..8883
                     /gene="liaX"
                     /locus_tag="ACHDLD_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN3361961.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9180..9500
                     /locus_tag="ACHDLD_000197"
     CDS             9180..9500
                     /locus_tag="ACHDLD_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN3361962.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9500..9850
                     /locus_tag="ACHDLD_000198"
     CDS             9500..9850
                     /locus_tag="ACHDLD_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN3361963.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10017..10955
                     /gene="hprK"
                     /locus_tag="ACHDLD_000199"
     CDS             10017..10955
                     /gene="hprK"
                     /locus_tag="ACHDLD_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN3361964.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10970..11800
                     /gene="lgt"
                     /locus_tag="ACHDLD_000200"
     CDS             10970..11800
                     /gene="lgt"
                     /locus_tag="ACHDLD_000200"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN3361965.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11844..12869
                     /locus_tag="ACHDLD_000201"
     CDS             11844..12869
                     /locus_tag="ACHDLD_000201"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN3361966.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12884..13822
                     /gene="galU"
                     /locus_tag="ACHDLD_000202"
     CDS             12884..13822
                     /gene="galU"
                     /locus_tag="ACHDLD_000202"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN3361967.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13947..14387
                     /locus_tag="ACHDLD_000203"
     CDS             13947..14387
                     /locus_tag="ACHDLD_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN3361968.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14390..14992
                     /locus_tag="ACHDLD_000204"
     CDS             14390..14992
                     /locus_tag="ACHDLD_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN3361969.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15051..16157)
                     /locus_tag="ACHDLD_000205"
     CDS             complement(15051..16157)
                     /locus_tag="ACHDLD_000205"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN3361970.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16367..17368
                     /gene="ccpA"
                     /locus_tag="ACHDLD_000206"
     CDS             16367..17368
                     /gene="ccpA"
                     /locus_tag="ACHDLD_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN3361971.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17705..20050)
                     /locus_tag="ACHDLD_000207"
     CDS             complement(17705..20050)
                     /locus_tag="ACHDLD_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN3361972.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20329..21231
                     /locus_tag="ACHDLD_000208"
     CDS             20329..21231
                     /locus_tag="ACHDLD_000208"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN3361973.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21444..23219
                     /locus_tag="ACHDLD_000209"
     CDS             21444..23219
                     /locus_tag="ACHDLD_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN3361974.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23285..24871)
                     /locus_tag="ACHDLD_000210"
     CDS             complement(23285..24871)
                     /locus_tag="ACHDLD_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN3361975.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25122..25346
                     /locus_tag="ACHDLD_000211"
     CDS             25122..25346
                     /locus_tag="ACHDLD_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN3361976.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25488..27239
                     /locus_tag="ACHDLD_000212"
     CDS             25488..27239
                     /locus_tag="ACHDLD_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN3361977.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27239..29023
                     /locus_tag="ACHDLD_000213"
     CDS             27239..29023
                     /locus_tag="ACHDLD_000213"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3361978.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29349..30200
                     /gene="licT"
                     /locus_tag="ACHDLD_000214"
     CDS             29349..30200
                     /gene="licT"
                     /locus_tag="ACHDLD_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN3361979.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30293..32179
                     /locus_tag="ACHDLD_000215"
                     /pseudo
     CDS             30293..32179
                     /locus_tag="ACHDLD_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(32219..33121)
                     /locus_tag="ACHDLD_000216"
     CDS             complement(32219..33121)
                     /locus_tag="ACHDLD_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3361980.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33352..34755
                     /locus_tag="ACHDLD_000217"
     CDS             33352..34755
                     /locus_tag="ACHDLD_000217"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN3361981.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34755..36188
                     /locus_tag="ACHDLD_000218"
     CDS             34755..36188
                     /locus_tag="ACHDLD_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN3361982.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            36333..37259
                     /gene="trxB"
                     /locus_tag="ACHDLD_000219"
     CDS             36333..37259
                     /gene="trxB"
                     /locus_tag="ACHDLD_000219"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN3361983.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37473..39200
                     /locus_tag="ACHDLD_000220"
     CDS             37473..39200
                     /locus_tag="ACHDLD_000220"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN3361984.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39215..39565
                     /locus_tag="ACHDLD_000221"
     CDS             39215..39565
                     /locus_tag="ACHDLD_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN3361985.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(39657..40517)
                     /locus_tag="ACHDLD_000222"
     CDS             complement(39657..40517)
                     /locus_tag="ACHDLD_000222"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN3361986.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            40609..41037
                     /locus_tag="ACHDLD_000223"
     CDS             40609..41037
                     /locus_tag="ACHDLD_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3361987.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            41199..41375
                     /gene="rpsU"
                     /locus_tag="ACHDLD_000224"
     CDS             41199..41375
                     /gene="rpsU"
                     /locus_tag="ACHDLD_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN3361988.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            41402..41848
                     /locus_tag="ACHDLD_000225"
     CDS             41402..41848
                     /locus_tag="ACHDLD_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN3361989.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(42002..43381)
                     /gene="brnQ"
                     /locus_tag="ACHDLD_000226"
     CDS             complement(42002..43381)
                     /gene="brnQ"
                     /locus_tag="ACHDLD_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN3361990.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            44087..45058
                     /locus_tag="ACHDLD_000227"
     CDS             44087..45058
                     /locus_tag="ACHDLD_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN3361991.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            45082..47274
                     /locus_tag="ACHDLD_000228"
     CDS             45082..47274
                     /locus_tag="ACHDLD_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN3361992.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            47291..47767
                     /gene="ybeY"
                     /locus_tag="ACHDLD_000229"
     CDS             47291..47767
                     /gene="ybeY"
                     /locus_tag="ACHDLD_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN3361993.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            47751..48146
                     /locus_tag="ACHDLD_000230"
     CDS             47751..48146
                     /locus_tag="ACHDLD_000230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3361994.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            48161..49060
                     /gene="era"
                     /locus_tag="ACHDLD_000231"
     CDS             48161..49060
                     /gene="era"
                     /locus_tag="ACHDLD_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN3361995.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            49203..50015
                     /gene="recO"
                     /locus_tag="ACHDLD_000232"
     CDS             49203..50015
                     /gene="recO"
                     /locus_tag="ACHDLD_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN3361996.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            50399..51316
                     /gene="glyQ"
                     /locus_tag="ACHDLD_000233"
     CDS             50399..51316
                     /gene="glyQ"
                     /locus_tag="ACHDLD_000233"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN3361997.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            51318..53393
                     /gene="glyS"
                     /locus_tag="ACHDLD_000234"
     CDS             51318..53393
                     /gene="glyS"
                     /locus_tag="ACHDLD_000234"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN3361998.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17361 a         9464 c        11378 g        15228 t
ORIGIN      
        1 atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga ttttgattcg
       61 ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta
      121 atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt atcaggctgt
      181 tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct
      241 ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta
      301 caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga
      361 aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat
      421 cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc
      481 agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt
      541 ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac ccgtagcacg
      601 tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc
      661 ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct
      721 tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat
      781 gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca
      841 aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc
      901 gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga
      961 aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt
     1021 tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag
     1081 ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa
     1141 atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta
     1201 ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc
     1261 aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg
     1321 aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct
     1381 gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga gatctctcct
     1441 aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc
     1501 gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg
     1561 actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactgtc
     1621 acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga agcttcatta
     1681 gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc
     1741 gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc
     1801 caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc tgcttctaca
     1861 ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat
     1921 gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc
     1981 cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg
     2041 ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca gctttactac
     2101 tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca
     2161 aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt tatttgcttt
     2221 tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat
     2281 atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt
     2341 tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg
     2401 gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta
     2461 gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca ggattggcct
     2521 taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg acaccaggga
     2581 ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct
     2641 atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt
     2701 caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca
     2761 atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga
     2821 ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa
     2881 aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa
     2941 tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc tacatgtctg
     3001 gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac
     3061 tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat
     3121 gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat
     3181 taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg
     3241 tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg
     3301 cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt
     3361 gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca
     3421 gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga
     3481 gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt
     3541 gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag ctcgtatcag
     3601 cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa ctaacaagat
     3661 atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga
     3721 cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc tgtattatgg
     3781 aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa tcactgcgat
     3841 gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc ggatgaatga
     3901 cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag acatctatgg
     3961 tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa
     4021 cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa
     4081 agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga
     4141 tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc agcagcagcg
     4201 tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac
     4261 aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga
     4321 gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa
     4381 gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga agatattcat
     4441 gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa
     4501 cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga
     4561 aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga
     4621 taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg
     4681 cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg
     4741 tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt
     4801 gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa
     4861 agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta
     4921 tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga
     4981 ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt
     5041 catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt
     5101 gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac
     5161 gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt aggaaaagag
     5221 gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga acagtcaaac
     5281 aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat gcaagctttt
     5341 gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc ttttcatcat
     5401 ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat tttttatttg
     5461 tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac ctgtcatcga
     5521 gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac tgatgaacac
     5581 ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc aaatagaaac
     5641 agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg aatggcagct
     5701 agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca ttggcgccat
     5761 gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca tcaatatcgt
     5821 tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc tattaaatat
     5881 atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag atggatggaa
     5941 tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat
     6001 ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg aactgaatta
     6061 tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg
     6121 aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc
     6181 agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag atgtgattgg
     6241 ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt
     6301 atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat cattggaatt
     6361 tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct cggtcctctt
     6421 ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta
     6481 gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc
     6541 atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg gccgatactg
     6601 gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt
     6661 tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta
     6721 ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt agaactagaa
     6781 gaattatctg gcttagataa gactttagcg attgatttta gattacttac gagatcgttg
     6841 cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc tagtgggaca
     6901 ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc
     6961 gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga
     7021 agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc
     7081 aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt gtccgcacta
     7141 cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa
     7201 ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat
     7261 aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa
     7321 gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga agaaggctta
     7381 gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa agaagcagac
     7441 aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga taaacttgaa
     7501 aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag acaagatcaa
     7561 gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt
     7621 ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga gaaacaggaa
     7681 gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca
     7741 cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga tacactgtta
     7801 gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca atggtctgag
     7861 aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga tcaagcaaca
     7921 gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc
     7981 ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga atggaaagat
     8041 gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt ttactatgaa
     8101 gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg
     8161 tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa aatgggtgct
     8221 gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga tcatatctcc
     8281 ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc taaacgagta
     8341 tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac attggaagct
     8401 aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt aactgcaacg
     8461 atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat
     8521 tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga aaacctttct
     8581 gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta
     8641 gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat cggtttggaa
     8701 ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta cgaagtagtt
     8761 cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa
     8821 atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga tacagataaa
     8881 taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tgtaaaatag
     8941 cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg aacaccgttt
     9001 accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt tcagaaaaag
     9061 ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa agacaccgat
     9121 taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag aggtgagaca
     9181 tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg actttagcag
     9241 gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat gtattcctga
     9301 gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg attatcccat
     9361 ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa aaaaacaatt
     9421 atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg
     9481 acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata cgttaacatt
     9541 catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt ggacggccgt
     9601 tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact
     9661 gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca atgcagcgat
     9721 tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt cattcggtgc
     9781 agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg aacataacag
     9841 tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt agaatcagga
     9901 atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt
     9961 tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt
    10021 cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc gtgacaggcg
    10081 acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca ggactagagt
    10141 tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa
    10201 tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga agattatgta
    10261 gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa gaaatgatcc
    10321 aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact tctcgtttat
    10381 taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt gtccacggtg
    10441 ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa
    10501 gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac gatcgagtag
    10561 atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc ctggaacatc
    10621 tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt gcgagtgcgg
    10681 tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca aaagataaga
    10741 aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg ggcattccac
    10801 aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcga
    10861 tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa
    10921 gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata tgttagcaca
    10981 aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg cattgatcat
    11041 cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc gggtaggatt
    11101 aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca ttattggcgc
    11161 tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa tccaaatatt
    11221 caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg cgatcacgct
    11281 gtattttttt actaaatatc attttatttc tttgtggact tttttagata tcgctgcacc
    11341 aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc acgaagcata
    11401 tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat tcatcattga
    11461 taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat cactttggag
    11521 tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg
    11581 cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta ttgaaggatt
    11641 acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat tatctgctgt
    11701 gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa atttaccgga
    11761 ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc gttaaaaatg
    11821 aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt
    11881 ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg atttggggac
    11941 atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt tatttaccag
    12001 atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca gtaaaagacg
    12061 cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca aaagaactag
    12121 tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta gaacagggaa
    12181 cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa agaaaagctg
    12241 ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac atcactacga
    12301 tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg ttcatgaata
    12361 attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg gcagctttga
    12421 aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc gataatgcaa
    12481 aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg
    12541 ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt acttgcacaa
    12601 gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag cccctagaag
    12661 aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg
    12721 aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt
    12781 atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat ggaaaaaaag
    12841 aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag ttaaaaaagc
    12901 agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag ctatggcaaa
    12961 ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag aagcgttgaa
    13021 atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca
    13081 ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt
    13141 gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa
    13201 gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac catttgtcgt
    13261 tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat taatcgatga
    13321 ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc
    13381 aaaatacgga attatcaatc caggagaagt attggaagat gggttataca atgtcaaaaa
    13441 ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta ttggacgtta
    13501 cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag ccggaaatga
    13561 aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa
    13621 attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa caagtattga
    13681 atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc tgtctttagg
    13741 taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa taacagaaat
    13801 caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct aattgaataa
    13861 aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa
    13921 aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga ttgatcgcag
    13981 caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa
    14041 ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa atcgtcacaa
    14101 aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt aatgaactgc
    14161 tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt
    14221 taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg
    14281 gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg acagcgatga
    14341 aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa tggctaaaaa
    14401 aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg ccgcgttatt
    14461 gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag cggatgattt
    14521 gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg agttaacagg
    14581 tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag caggtagtgt
    14641 caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg atatcttgtt
    14701 aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg cagaagatat
    14761 gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag atatcatgat
    14821 tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc
    14881 aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga
    14941 agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact aaaatcacaa
    15001 atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc ttatttttct
    15061 aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct tgtcacatat
    15121 acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg ttcaagagag
    15181 aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg gaattcgtgg
    15241 acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta gccatagctg
    15301 ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg acggacggct
    15361 tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg gaagtcggtt
    15421 ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca aacaacgcca
    15481 agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt accatgaggg
    15541 cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga aacaccttgt
    15601 tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact
    15661 gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat cagtttgtct
    15721 ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag atcagtgtaa
    15781 gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt caaggcgttc
    15841 ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt tccgatgatc
    15901 tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact actgttttga
    15961 gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag agggataaaa
    16021 agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc gatgtggcca
    16081 gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg tagttcattg
    16141 atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt caataattgt
    16201 accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa attttcatga
    16261 aaacagagat aaaacggttg caattcttga gattatttga tattctattt tagaaatgta
    16321 agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg aaaagcaaac
    16381 aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg tttcacgtgt
    16441 cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg aagttatcga
    16501 tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac
    16561 agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag cacgtggtat
    16621 cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag atggcaacga
    16681 ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg gcgtgatctt
    16741 catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa aaactcctgt
    16801 tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca ttgattatgt
    16861 atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt
    16921 tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag gctataaaga
    16981 agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag cacaatacaa
    17041 cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa cagctgtcta
    17101 tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc gtggcgtgaa
    17161 agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag aagtagctcg
    17221 tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat ctatgcgcct
    17281 attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct tgccttatgg
    17341 aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg ttgtgtgcct
    17401 gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat gtttgatgaa
    17461 tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga aaaggggtta
    17521 tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa gtggttgaaa
    17581 ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt caccaccatc
    17641 aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg tgtattctat
    17701 taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt gcataagctg
    17761 aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca aatttaaagg
    17821 cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct ggtgtttgga
    17881 tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca gatacttttt
    17941 tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg tcaggttttg
    18001 cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc cttgctttgc
    18061 tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata gacggagtag
    18121 agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg acccaatctg
    18181 cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta gtgatttttg
    18241 cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc gaatagatcc
    18301 ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg atggaatcga
    18361 tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg ttggtctgtt
    18421 gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa ttgtcatttg
    18481 ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat ccaccggaat
    18541 tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct agtgactcac
    18601 ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc tcgccagcat
    18661 tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat ccgataagcc
    18721 ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca gcttgacgtt
    18781 ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca acgaatccgt
    18841 agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga ctgccagcac
    18901 catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca tagacatctt
    18961 tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt gccttttgat
    19021 aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca gccaattgtt
    19081 gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct ctgtcagtct
    19141 gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt
    19201 attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa tcttttctgg
    19261 caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga ctatacgtga
    19321 tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca tcttttgcat
    19381 ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt cccttattat
    19441 tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt
    19501 tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt gtttcatctg
    19561 taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa gagccaatac
    19621 cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct tggtgtttat
    19681 cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa atcttatcag
    19741 atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta ttatctgcat
    19801 aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg
    19861 gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca atacctagag
    19921 ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa actctaaaaa
    19981 taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt cgagtatttt
    20041 tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata gcaaataata
    20101 gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa gagttaaaac
    20161 gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag aaaatttcag
    20221 ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc atcttaccaa
    20281 tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat gttattaggc
    20341 tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga agcagccagt
    20401 tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag aagaaaagcc
    20461 atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg tctaacaaat
    20521 atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc agaaatgttt
    20581 ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt aggcgctact
    20641 caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg aattgctaga
    20701 attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat cgctttggag
    20761 acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc gcaaatcatc
    20821 gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca tacgaatgat
    20881 gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga taagatcatc
    20941 gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca aggttcgcat
    21001 aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct gaataagatc
    21061 gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta tgtaggcaaa
    21121 gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa agcgcaaaca
    21181 ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta agattttcta
    21241 tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt taagaaacaa
    21301 aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt ttaaatttag
    21361 acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa
    21421 aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc aaccaactaa
    21481 aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt attcgtttgt
    21541 atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat gaccagctgc
    21601 tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat acgactacaa
    21661 cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt
    21721 cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt
    21781 ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt
    21841 ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc
    21901 cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa tacggttcta
    21961 gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca
    22021 ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg
    22081 tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac
    22141 tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg
    22201 gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac ggtattgcaa
    22261 ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag
    22321 atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa
    22381 ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat
    22441 atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct
    22501 tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa
    22561 ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacaa
    22621 tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc
    22681 tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt
    22741 tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat
    22801 tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt
    22861 tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg
    22921 ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat
    22981 ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac
    23041 tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa
    23101 aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa ttcatcgtag
    23161 taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct tctaaataaa
    23221 aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca
    23281 gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt ttgtttcctt
    23341 tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag
    23401 cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag aaactttcga
    23461 tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa
    23521 gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca
    23581 tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc tgggattcat
    23641 tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa
    23701 cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg
    23761 ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta ccagtaatag
    23821 tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag ataccagatt
    23881 ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa gcttcataca
    23941 gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca
    24001 atagcagtgc attgactaga aaagccattg acaactggat attagaatcc caagtagaaa
    24061 aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa
    24121 ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc
    24181 cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga
    24241 taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa
    24301 cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt
    24361 ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg
    24421 gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca atatctgttg
    24481 ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta cttgtgcgtg
    24541 ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct gccatatact
    24601 gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa tattggaaat
    24661 tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca actgctacta
    24721 acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc tttggtacat
    24781 caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct
    24841 tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta tttatctacg
    24901 attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac gcctaatttt
    24961 ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta aaaaaaccac
    25021 tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata
    25081 agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca acaggtattg
    25141 tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc
    25201 gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat
    25261 caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag atgatgcagc
    25321 aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg ggctgatttt
    25381 ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag caaaaaagac
    25441 aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg tcgattttta
    25501 gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg gttttctcgc
    25561 tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg
    25621 agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc ctattgatta
    25681 tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg
    25741 cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca gaaatggaca
    25801 atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg aatgatttga
    25861 atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca gtgattacgg
    25921 gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca ctgattgcgc
    25981 tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa ctccatgatg
    26041 cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca
    26101 ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg gattttgaag
    26161 aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac tccttatttg
    26221 atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa
    26281 agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt agttatatcg
    26341 gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa
    26401 atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac
    26461 tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc tcttttaatt
    26521 atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa gaaggagaaa
    26581 cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc
    26641 gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt
    26701 cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc ttgttttcga
    26761 tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc
    26821 atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa ggttatgata
    26881 ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg atttcgattg
    26941 ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc tctgcagtag
    27001 atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa
    27061 ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg
    27121 atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta ggcggctggt
    27181 atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat
    27241 ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaaaaagat
    27301 catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg ttgctatctt
    27361 atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat
    27421 ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt ttgctggtat
    27481 gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt atttgtattc
    27541 catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa agctgcatac
    27601 actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc gagtgacgaa
    27661 tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga ctgggatctt
    27721 tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac
    27781 tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat tcagttctcg
    27841 tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga atgaatcgat
    27901 ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga
    27961 acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga attcgattct
    28021 gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt tgactttgtt
    28081 tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgaccta
    28141 tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca tcttcacaga
    28201 tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat atgcaccaca
    28261 acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc gcaatgtaag
    28321 cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg ctaatccggg
    28381 agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca tcaacgtatt
    28441 gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg acattcgaga
    28501 atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt
    28561 ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag acgaacagat
    28621 acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac caaaaggata
    28681 tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac agctgatcac
    28741 ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag caacagcaaa
    28801 tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc gtcaaggacg
    28861 gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat tgattttagt
    28921 tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg
    28981 tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt tgtgacagta
    29041 gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt tttcatctgt
    29101 gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt cagaagtgcc
    29161 taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata aagaaaacga
    29221 tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc catggtttca
    29281 tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat catggaaagg
    29341 agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc agggatgaaa
    29401 gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc
    29461 cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg ctttcacaac
    29521 agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct tgtgaaatca
    29581 ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat attgcattga
    29641 cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta ccaaatgtat
    29701 tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa aaaacagtga
    29761 agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga tttatcgcat
    29821 tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac ctgtttacta
    29881 taacaataca ggatattcta aatatcgtcc gctattacta taagattaca tttgatgaag
    29941 attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca cgcgtcctag
    30001 atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt attcaaaaaa
    30061 aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc gagacgttca
    30121 actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc
    30181 atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat gaaataaggg
    30241 tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag aaatgggaaa
    30301 ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa atatcaatgg
    30361 tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga
    30421 tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag ggcagtatca
    30481 agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag caggaaacct
    30541 tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc ttattgatgg
    30601 aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga tgattcgtgg
    30661 actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca catttgttat
    30721 gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg gtttaacagc
    30781 tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag cattaatgaa
    30841 tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt
    30901 ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc cctggattgc
    30961 gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag ctagccaaat
    31021 tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg ttccattctt
    31081 caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat tcacattcgc
    31141 aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag ttatttatgg
    31201 agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt
    31261 accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga cacctgttgc
    31321 agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc ttagaaatcc
    31381 aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga ttacagagcc
    31441 agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct gtgtcggtgg
    31501 tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc aaggtggact
    31561 aggcatcttt acaattccca actatattag accagatggt gatttaacag atgtgattaa
    31621 tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt ttttcttttg
    31681 gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag aaattgtaaa
    31741 aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag cttttgcaca
    31801 aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt
    31861 tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg tttctgaaac
    31921 agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag ggaaatattt
    31981 tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc tacgcttcga
    32041 caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtgatcg tcaccaatta
    32101 cacggattat ttggatattt tggaaaacca aggggagacg gtcgggccag acgatgcact
    32161 tatcaccgca ctgacctgaa cggttaggtg aagaatggac taggcttaac tattcttttt
    32221 attccacttc tttattttca aaattgaaag gctctttcat gatggctgag ctgatagggc
    32281 gtttatcgaa tttttgaccg acagaaatta gatggactaa attctgttga taattttctg
    32341 caaaaacaca actatataac acgtctagaa gataacaaat tgatgtattg atcgtaaaat
    32401 taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct gtactcattg
    32461 cagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga ttttgtttca
    32521 atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc agaatcccgc
    32581 acatatcact cgtgcaattg tacgcttcat agaatgattc accaaaagtg gtgacaatcg
    32641 tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc aaagtattcg
    32701 agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg gctttttgca
    32761 gttggtcatg atgaatgaga gaaagtgtat catgaatcgt catctgttct aaagaagcca
    32821 ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc gcatctacgt
    32881 cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga aaactgaccc
    32941 agccatcaaa acccattttt tttgctacac ggatgagtgt agaggggtgg acataacatc
    33001 gctcagcaat cgcttggatg gtcatttcgc caagtgctgg agaatcgctt aagagaaact
    33061 ggatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca cgtaataaca
    33121 tgtagatacc ttcttcttat ttttttacgt aaattataac ttttttgaaa atgatttgta
    33181 aacggattta tactatataa cttaaaatag acattttttg acaagatatt tacaaaaatc
    33241 cactagaaaa cagtaaattt tgtaaatgtt actggaaaga aacagatttc tattgtttgt
    33301 tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa catggcaaaa
    33361 ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt gatggaagga
    33421 tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga tgtagaagaa
    33481 ggaaaagaaa agcttgagat cgttagtcag atgtctcagc gtatgtggga tgcttctccg
    33541 tatgaagtca agatctatgc aactctggat cgggaagaag cattgaaaga tgcggatttt
    33601 gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga gcggatccct
    33661 agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt caaagctttt
    33721 cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg tccaaatgct
    33781 tggctgatca atttcgcgaa cccaagtggg atggtgacag aagcagttat cagatatggc
    33841 aaatgggata aagtgattgg attatgcaat gtgccagtga tggcgatgat gacagaacca
    33901 gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct aaatcatttc
    33961 cattggcaca aagtagcaga taataaagga aatgaccgca cggcagaatt gattgatcgt
    34021 ttatacgatg aagagaatgg attaccgaaa aatatctttg atatcccgtt tttcaaagaa
    34081 caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta tcgtgaagaa
    34141 gaaatgctgg aacatgcttt agaagaatat caaacgattg gtacaagagc gcagcaagtg
    34201 aaagaaacag aagcagagct atttgaatta tacaaagatc cagaattaga ccataaacct
    34261 gaacaattgc aaaaacgagg tggcgcttat tattcagacg ctgcctgtga aacgattgct
    34321 tcaatctatg gaaataaaga aacacagatc gtggtatcta caaagaatga tggagctgtt
    34381 cctgacctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc acaaggagcg
    34441 agaaatgtag cgtttggttc tcttcctgct gctgaaagag gctggttgca agtaatgaag
    34501 aatatggaat tgctgacgat cgaagcagct gtgactggtg attatgggac agctttacag
    34561 gcgtttacga tcaatccact tatacgatca ggaaagacag caaaacggat catggatgag
    34621 ttgtttattg cacaccagca gtatttaccg caattcaagg agacaatcag ccgattaaaa
    34681 gaagaaggaa tcgaagttca agatgaactg gcaagagaat tatcaggtaa tacacatata
    34741 atggaggaag tataatggga ttcagaaaag attttttatg gggcggtgct acagcagcca
    34801 atcaatgtga gggcggttat gatgaaggtg gacgcggact agcaaacgta gatgttgtgc
    34861 caatcggaaa agaccgtttt cctgttatca ccggaaagaa aaaaatgttt gaatttgatg
    34921 aggatcattt ttatccagca aaaattgcaa tcgatatgta tcatcgttat aaagaagata
    34981 ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc tggagtcgta
    35041 tttttccaat gggggatgag aaagagccga atgaagctgg cttgaaattt tatgaggatg
    35101 cctttaaaga atgccaaaag cacggaattg agccacttgt gacgattacg cattttgatt
    35161 gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc cagagaactc gttggttttt
    35221 atgaaaatct gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa tattggctga
    35281 cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg ctgtattttg
    35341 aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag ttggttgcaa
    35401 gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta ggctgtatgc
    35461 tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg gcttctagaa
    35521 aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa tatcccgctt
    35581 atgcattgaa agaaatggag cgaaatggca ttcagatcga aatggaacca ggggatgccg
    35641 cattgctgaa agaacatact gttgatttta tctctttttc ttattatgct tcccgagttt
    35701 cgactaccga caacgaactc ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa
    35761 atccatatct gcaagcaagt gaatgggggt ggcaaattga tccattaggc ttgagaataa
    35821 cgatgaatga tatttatgat cgttatcaaa aaccattgtt tattgtagaa aacggcttag
    35881 gtgcagtcga tacgccagat gaaaatggtt atgtggaaga tgattaccga atcgattacc
    35941 tggcagcgca tatccaggcg atgaaggatg cagtagaact agacggagtc gatctattag
    36001 gctatacgac ttggggatgt attgatttag tatcagctgg taccggtgag atgaagaaaa
    36061 ggtacggatt catttatgtc gatcgtgata accaaggaaa tggcacatta gcccgttcaa
    36121 agaaaaaatc attcaactgg tataaaaaag taattgattc aaatggagaa aatctttcga
    36181 acgattgatc aatgatttat aaaagtctat tgtttttcag cggatttttc gagtataata
    36241 aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc tgtaaagtat
    36301 gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt attggcgcag
    36361 gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc gtattgatga
    36421 tcgaacaagg tgcccctggc ggccaaatga acaatacggc agaaatcgaa aattatcctg
    36481 gattcgattc gatcatgggc cccgatctag cttataagat gtatgaaagt gtctcaagat
    36541 ttgggacaga aaatgcttat ggaatcgttc aagatatcaa ggatcacggc agccataaag
    36601 aagtcatctg tgaagataaa agttacgaag gaaaaacagt cattatcgca acaggatgta
    36661 tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgaggc gtttcttact
    36721 gtgcagtctg tgatggtgcg tttttccgta acaaacgtct agtagtcatc gggggtgggg
    36781 actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta gtgatcgtcc
    36841 accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt gcaaatgaaa
    36901 aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa atggtagtga
    36961 caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca gcagacggag
    37021 tattcatcta tgttggactg gatccattaa ctgcaccttt tagagaagca ggtttgacga
    37081 acgaagctgg ctggattcca acagacaacg aaatgaaaac gaatatgcca ggcgtttttg
    37141 ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt ggtgacggcg
    37201 gtattgctgg acaacaagtg tataaatact tggagtcttt agaagaaaca acagcataag
    37261 tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt cgcgtgaaat
    37321 ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa tcctttcttg
    37381 atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct atacttatta
    37441 agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg tatgaacaat
    37501 gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat ctgggtgaag
    37561 atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact gcaggtatgc
    37621 ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga caagcaacag
    37681 aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga ggtgtcgcta
    37741 tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc aaaacactag
    37801 cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca gagctttctt
    37861 ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct cacaacccag
    37921 cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct cctgcagacg
    37981 cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc gaagtattat
    38041 ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa gtggacgctg
    38101 cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa acaatgggga
    38161 aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg ggcgaacgtg
    38221 cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct gtggcagatc
    38281 cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt gaatatgcta
    38341 tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct gatgcagacc
    38401 gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact gggaaccaaa
    38461 tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc actttaccag
    38521 aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca attgcggaac
    38581 actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct gaaaaaatcc
    38641 aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa agttatggtt
    38701 atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt ttattagcag
    38761 aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg caaacgattt
    38821 ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc ggacaagaag
    38881 gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg acagaatttg
    38941 ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca gaccgtgaag
    39001 gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca ttggaagaaa
    39061 atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt tatattggtg
    39121 taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa gcgattatca
    39181 aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa actttagaag
    39241 tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat caaaagtaga
    39301 gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa ttctgctata
    39361 tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa tggagcagtc
    39421 tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt ctcatcgcga
    39481 gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt tgaaccagac
    39541 actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact ggagtgtgac
    39601 aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa aaatgattaa
    39661 tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc taaagcattc
    39721 aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac tacacatcct
    39781 agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc gatatcggaa
    39841 taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat attatttaaa
    39901 atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca cagctgtttg
    39961 ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt atttgctaaa
    40021 aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac atctgcttct
    40081 aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat tgctttgatc
    40141 cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat tggcttgata
    40201 ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact aaataattga
    40261 tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa tgtatgcagg
    40321 acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt ttcatctgtc
    40381 acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat agtagcttgc
    40441 gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt aacgatttct
    40501 gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt attataccat
    40561 actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat gaaaaaacag
    40621 acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata tactaaaaaa
    40681 agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc taaagaagtt
    40741 tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta ccggaatctc
    40801 catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga aatgaagttt
    40861 cgttttcatt gctgtcaggg ggaaatcggc catcaccatc attttatctg tacagtgtgc
    40921 ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca gctaggtgga
    40981 tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg tcaataagga
    41041 tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg acggtcaaaa
    41101 cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt tcaataacaa
    41161 ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca gtcgttcgca
    41221 aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca aaagcgggta
    41281 ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga cgcaagaaaa
    41341 aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg gagttggtag
    41401 tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag cgaaagataa
    41461 ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc agattaaaga
    41521 aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa tgaaacagcg
    41581 acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg aaaaagtaaa
    41641 aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg aagaaattcg
    41701 acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag aatttggcaa
    41761 agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc aagtgaatgc
    41821 gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac agtgattgtc
    41881 cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa gaataagatt
    41941 ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt gtttgatttg
    42001 gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga taggcgtaat
    42061 aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca agtcctaatg
    42121 cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca gtttgctgac
    42181 tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca gaaaagaaca
    42241 tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag gaaagcaaga
    42301 ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat tggatgatat
    42361 tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc agccatgtca
    42421 tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca acgagtccca
    42481 tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca ccgaagttac
    42541 ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat aaaggcatct
    42601 tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact tctaacgcaa
    42661 cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta aaaccaagat
    42721 cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca tcaaccgtgt
    42781 tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct gcagcagatt
    42841 ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct aataatgcaa
    42901 gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga tgtacagtaa
    42961 ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat gaatcatatt
    43021 tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc cgcggtgtac
    43081 caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca taccattttc
    43141 cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca gctaagatag
    43201 ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg atccagtttg
    43261 atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg aagaacatcc
    43321 cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt gtttcttcca
    43381 taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc agaaactaga
    43441 aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa aaaagccctt
    43501 ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc gcttaaaaaa
    43561 gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata atggaggcag
    43621 ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat tcgatttatt
    43681 gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta ctgttttcct
    43741 cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt tttgaaatgc
    43801 aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc cagagaatac
    43861 ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat gatcagcttt
    43921 ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt ttcttggtac
    43981 ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt cttctgagta
    44041 tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga cggaagagtc
    44101 tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt taggtgcaca
    44161 cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata cacgtggaga
    44221 aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag tcattcggac
    44281 attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg tgacggcact
    44341 gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg aagagatcgt
    44401 caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga aatatgtcga
    44461 atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag gaaaaacttt
    44521 tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa aaatcatttt
    44581 gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg atctaaaaga
    44641 aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt ttggattaga
    44701 tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag cttacatgcg
    44761 ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca cgacagttgc
    44821 tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg tcaatggaga
    44881 tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg cagaacgaac
    44941 attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg ttgttcggca
    45001 ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc aagaataagc
    45061 tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca agaaaaactt
    45121 ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg cggcgttaca
    45181 tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc agaagaaagt
    45241 atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag aaaactggca
    45301 gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga ccagaacaat
    45361 cggatcaagc aactatttga attgatcgat aaaacgaatg atgccatcaa caaatcttat
    45421 gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac tacagaagag
    45481 cggatcgccg cgttgaagaa aaattttgaa aatgtgaatg cagaaaatgt tgcgttttat
    45541 caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac tgagatccat
    45601 actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca agttcgtgaa
    45661 agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt gaatgtcaca
    45721 ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt gaatgacgta
    45781 ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt acaaccagtg
    45841 atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc ttcagctatg
    45901 aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc gctaattgcc
    45961 atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact tcaattccaa
    46021 aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc agtcagcata
    46081 gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat cccgctgttt
    46141 tatcccgctg cttttgcacc acttgtttta agctttttct tgaaccgaag agcagggatc
    46201 atggctggta ttttccaagc agtatctgca cttttcattt tttatgattc gattggcacg
    46261 aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc aacgattgtc
    46321 agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat tatttttcct
    46381 gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga tgcaacgact
    46441 tggttaatgc ttgtctgcgg gttcactggt gcactcttct cattcttgct gacgatcggt
    46501 ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt aaatgaactc
    46561 agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg tacttatcat
    46621 catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg cggacgttct
    46681 ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca tgctagcttt
    46741 ttcgttgaga acttgccttc aggagcggaa aacccgcaca atttcttatt gccagaagac
    46801 agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga agattataat
    46861 atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct aatgcgttat
    46921 ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt ccgttatcct
    46981 ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag ttgtgaggct
    47041 gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt ccataatctt
    47101 atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt gaaagaaatc
    47161 cgaaaagtgg aaaaatcatt gatcagcggt ttaagtagca ccttccattc tcggatcaaa
    47221 tatcctaaaa tgaaaacaga agttgagaag ataaaggaag aacaagaaga atagaaagaa
    47281 gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag aagagaaaat
    47341 gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc cagaggaaac
    47401 agaaatgtct gttactttca tggacaatgc ggcaatccaa gtaatcaatc gtgattaccg
    47461 tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag gcgaagacga
    47521 aatcccagtg atttttgaag atggagagaa tccattgcct cgtgtactag gagacatcat
    47581 gatttctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat atgatcggga
    47641 acttggcttt ttagcggtac atggattctt gcatatcaac gggtatgacc atatgacacc
    47701 agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct atggactcaa
    47761 aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt gctgtccaag
    47821 gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg ttcggtatcc
    47881 ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta tggctgcttt
    47941 tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa aatgtagtgg
    48001 acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat atggcagctg
    48061 gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta tttgtcccta
    48121 aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac ataaatcagg
    48181 gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga accgaatcgt
    48241 gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata aaatccaagg
    48301 agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa tccataaacc
    48361 aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga aagaagtgga
    48421 tgcagtctta tttatgatca gtgctgatca aaaacgcggc cggggagacg attttattat
    48481 tgaacgtcta aaaaacgtgc attctccagt ttatctgatt atcaataaaa tcgataagat
    48541 ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc cttttgcaca
    48601 agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg atgtattagt
    48661 cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg atcatcctga
    48721 atactttatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac gagacgaagt
    48781 gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg ataaaatcca
    48841 tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta tcggcaaagg
    48901 cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa atcttttagg
    48961 agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag acaaaaaagt
    49021 ctatctgcaa gactttggct accgaaaaga cgaatactga tattctgaac ttcttatagg
    49081 ttaagtatag aaataaactg gataaaaatc cgcacgttga gaaaaacggc ggaagatcga
    49141 aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt aggagggaga
    49201 atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat tacaaagaaa
    49261 aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt tatatcaaag
    49321 gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc gaagcagtct
    49381 atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa gacgtccatg
    49441 cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacctat ttgctaaatc
    49501 tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa tttacgcaac
    49561 aagctctgca gcggatggat caaggagatg atgcagaaat catcacgaat atctttgaag
    49621 ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc gtatgcgggg
    49681 aaacaaaagg aaaatttgac tattcttcca aatctggcgg tgtgatatgc gagaaacact
    49741 ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc gttcgaatgt
    49801 tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa acgaagcagg
    49861 caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat ctaaaaagca
    49921 aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca gagaaaaata
    49981 tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca aaaaaatcaa
    50041 aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa atagatcgtt
    50101 cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta cattgaccaa
    50161 gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga cccaaacgat
    50221 ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat tcggaagtag
    50281 ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga ctttttgtcg
    50341 ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg caaaacagat
    50401 gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt ggtcatcaaa
    50461 cggctgcatg ctgatgcaag cttatgacac agaaaaagga gcaggtacga tgagcccata
    50521 tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag agccatcccg
    50581 ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc atcatcaatt
    50641 tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag aaagcttgcg
    50701 tcttttagga attgatccat tagaacacga tattcgtttt gttgaagaca actgggagaa
    50761 cccatcgatg gggtgtgccg gtgtgggttg ggaagtatgg cttgatggga tggagatcac
    50821 acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta cttctgagat
    50881 cacttatgga ctagaacgtc ttgcttcata tattcaagaa gttgaaagtg tctatgatct
    50941 ggaatgggct gatggtgtga aatatggcga gatcttcaaa caaccagaat atgaacattc
    51001 caaatattct tttgaagtaa gtgaccaaga cttgctacta agcaactttg accgttttga
    51061 aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg attatatttt
    51121 gaaatgcagc catacattca atttattaga tgctcgcggt gcagtatccg taacagaacg
    51181 agcaggttat ctttcccgta tccgcaacat ggctcgtgct gtagccaaaa tctttgtggc
    51241 agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc cagaaaacat
    51301 gaaggaggct gaataagatg gcaaaagact tattacttga aatcgggctg gaagaaatgc
    51361 ctgctcatgt tgtcacacca agcatgaaac aattagaaga aaaaacagca aaatttttag
    51421 atgaaaacca attgacttat gatacgatcg aaacgttttc tacaccgcgc cgcttagcaa
    51481 tcaaagtaac aaacattcca gaacgtcaag cagacagtga agaagaagtc aaaggaccag
    51541 ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagctgaa ggatttgtcc
    51601 gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc gtggaatatg
    51661 tctatgtaac gaaatacact cacggcaaag aagcgaaaga agtattgaca ggtttagcag
    51721 atgtcattaa aagcctcact ttcccagtaa cgatgcattg ggcagaatac gattttgaat
    51781 atatccgccc tattcactgg atcgtggcat tgttagatga cgaagtaatc ccgtttgaga
    51841 tcttagatgt caaaacaggc agagcctctc gaggacatcg tttcttaggc gaagatgtga
    51901 catttgcgca tgcaaaagaa tatgaagaaa aattgaatga acaattcgtt attgcttcac
    51961 cgcaaaaaag acaagaaatg atcgttacac aagcaaatga attagccgaa aaaaatcact
    52021 gggttattga tctagacgaa gatttactag aagaagtcaa caatttagtc gaatatccaa
    52081 ctgtcttttc tggcgacttc gaagaaaaat atctttctgt tcctgaagaa gtactagtca
    52141 cttctatgaa agaacatcaa cgttatttcg aggtacggaa cgatcaaggt atgctgcttc
    52201 ctcattttat ttctgctcgt aatggtgatc gggtaaaatt ggaaaatgtg gttcgaggca
    52261 atcaaaaagt attgacggct cgtttagaag atgcggaatt cttttataat gaagacaaga
    52321 aagcttcaat cgaccaatac gttgaaaaac taaaaactgt tactttccat gaaaaaatcg
    52381 gtagtatcta tgagaaaatg cagcgggtac aagtcattgc ccgtatcatt ggcgaacaag
    52441 taggactatc tgaggaagaa ttgatggatc tgcaacgagc tgctgaaatc tataaatttg
    52501 atctagtaac caacatggta ggggaatttc ctgaactaca agggatcatg ggcgaaaaat
    52561 atgctctgct gaaaggcgag aaacctgctg tagcaacagc gattcgtgag cattacatgc
    52621 ctatatccag tgaaggagag ctacctcagt ctacagtagg ttctgtttta gcgattgcag
    52681 ataaattaga tagcattttt tctttctttg cagttgggat gattccaagc gggtcaaacg
    52741 atccttatgc cttacgtcgc caagcatacg gtgtcgttcg gatcatcgaa tcgaaaaaat
    52801 gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga tgttatcagt aaagatacgg
    52861 atcgtttcgg cattggttta tcagaagggc aacaacaggt gatcgacttt atcaaaggcc
    52921 gtctgcgcca attattgaca acgaagaata ttcgtcatga tgtcatcgaa gctgttttga
    52981 atgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc aagcagcatc
    53041 ttgcagatga agacttcaaa ccatcgatgg aagctttgac acgtgtcgtg aacttagcga
    53101 aaaaagcgga gcttgaaaaa caatcagaag tagaccctga actgttcgaa aatgaagcgg
    53161 agaaagaact gcacaaagca gtagaggcaa tgaaaaaaag ctttgctgac caaacgatca
    53221 acgaaaatta tgatcagctt gtaagccttc gcccactgat cgaaaattat tttgatcaga
    53281 cgatggtcat ggcagaagaa ccagcagtac gtaagaaccg attaacgcaa ttaagtcaaa
    53341 ttgctgccat ggctttgtcg ctagcaagtt tagatcaaat catcacaaaa taatttctaa
    53401 tatgtaaata aaccagagaa taggcacaaa a
//
LOCUS       DAZAOG010000005        50069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000005 DAZAOG010000000
VERSION     DAZAOG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.005"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..272
                     /locus_tag="ACHDLD_000235"
     CDS             <1..272
                     /locus_tag="ACHDLD_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3361999.1"
                     /translation="KETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYV
                     IEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(353..922)
                     /gene="thiT"
                     /locus_tag="ACHDLD_000236"
     CDS             complement(353..922)
                     /gene="thiT"
                     /locus_tag="ACHDLD_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN3362000.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(988..1090)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1096..2067)
                     /locus_tag="ACHDLD_000237"
     CDS             complement(1096..2067)
                     /locus_tag="ACHDLD_000237"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN3362001.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2161..3534)
                     /gene="glmU"
                     /locus_tag="ACHDLD_000238"
     CDS             complement(2161..3534)
                     /gene="glmU"
                     /locus_tag="ACHDLD_000238"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN3362002.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4037..4855)
                     /gene="purR"
                     /locus_tag="ACHDLD_000239"
     CDS             complement(4037..4855)
                     /gene="purR"
                     /locus_tag="ACHDLD_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN3362003.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5022..5846)
                     /locus_tag="ACHDLD_000240"
     CDS             complement(5022..5846)
                     /locus_tag="ACHDLD_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN3362004.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5819..6499)
                     /locus_tag="ACHDLD_000241"
     CDS             complement(5819..6499)
                     /locus_tag="ACHDLD_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3362005.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6522..7460)
                     /locus_tag="ACHDLD_000242"
     CDS             complement(6522..7460)
                     /locus_tag="ACHDLD_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN3362006.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7705..8556)
                     /gene="ispE"
                     /locus_tag="ACHDLD_000243"
     CDS             complement(7705..8556)
                     /gene="ispE"
                     /locus_tag="ACHDLD_000243"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN3362007.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8710..11283)
                     /gene="mprF"
                     /locus_tag="ACHDLD_000244"
     CDS             complement(8710..11283)
                     /gene="mprF"
                     /locus_tag="ACHDLD_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN3362008.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11424..12230
                     /gene="proC"
                     /locus_tag="ACHDLD_000245"
     CDS             11424..12230
                     /gene="proC"
                     /locus_tag="ACHDLD_000245"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN3362009.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12309..13865)
                     /gene="rny"
                     /locus_tag="ACHDLD_000246"
     CDS             complement(12309..13865)
                     /gene="rny"
                     /locus_tag="ACHDLD_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN3362010.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14276..15325)
                     /gene="recA"
                     /locus_tag="ACHDLD_000247"
     CDS             complement(14276..15325)
                     /gene="recA"
                     /locus_tag="ACHDLD_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN3362011.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15434..16675)
                     /locus_tag="ACHDLD_000248"
     CDS             complement(15434..16675)
                     /locus_tag="ACHDLD_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN3362012.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16915..17493)
                     /gene="pgsA"
                     /locus_tag="ACHDLD_000249"
     CDS             complement(16915..17493)
                     /gene="pgsA"
                     /locus_tag="ACHDLD_000249"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN3362013.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17702..18625)
                     /locus_tag="ACHDLD_000250"
     CDS             complement(17702..18625)
                     /locus_tag="ACHDLD_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN3362014.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19364..20650)
                     /locus_tag="ACHDLD_000251"
     CDS             complement(19364..20650)
                     /locus_tag="ACHDLD_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN3362015.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20643..21911)
                     /locus_tag="ACHDLD_000252"
     CDS             complement(20643..21911)
                     /locus_tag="ACHDLD_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN3362016.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22163..22624
                     /gene="mscL"
                     /locus_tag="ACHDLD_000253"
     CDS             22163..22624
                     /gene="mscL"
                     /locus_tag="ACHDLD_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN3362017.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22684..23130)
                     /locus_tag="ACHDLD_000254"
     CDS             complement(22684..23130)
                     /locus_tag="ACHDLD_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3362018.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23223..23468)
                     /locus_tag="ACHDLD_000255"
     CDS             complement(23223..23468)
                     /locus_tag="ACHDLD_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN3362019.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23584..24162)
                     /locus_tag="ACHDLD_000256"
     CDS             complement(23584..24162)
                     /locus_tag="ACHDLD_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3362020.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24239..24769)
                     /locus_tag="ACHDLD_000257"
     CDS             complement(24239..24769)
                     /locus_tag="ACHDLD_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN3362021.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24773..25633)
                     /gene="rlmB"
                     /locus_tag="ACHDLD_000258"
     CDS             complement(24773..25633)
                     /gene="rlmB"
                     /locus_tag="ACHDLD_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN3362022.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25626..26021)
                     /locus_tag="ACHDLD_000259"
     CDS             complement(25626..26021)
                     /locus_tag="ACHDLD_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN3362023.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26018..27430)
                     /gene="cysS"
                     /locus_tag="ACHDLD_000260"
     CDS             complement(26018..27430)
                     /gene="cysS"
                     /locus_tag="ACHDLD_000260"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN3362024.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27427..27963)
                     /gene="epsC"
                     /locus_tag="ACHDLD_000261"
     CDS             complement(27427..27963)
                     /gene="epsC"
                     /locus_tag="ACHDLD_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN3362025.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(28049..28288)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28285..29745)
                     /gene="gltX"
                     /locus_tag="ACHDLD_000262"
     CDS             complement(28285..29745)
                     /gene="gltX"
                     /locus_tag="ACHDLD_000262"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN3362026.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(29915..31072)
                     /locus_tag="ACHDLD_000263"
     CDS             complement(29915..31072)
                     /locus_tag="ACHDLD_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN3362027.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31106..32476)
                     /gene="radA"
                     /locus_tag="ACHDLD_000264"
     CDS             complement(31106..32476)
                     /gene="radA"
                     /locus_tag="ACHDLD_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN3362028.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32539..33066)
                     /locus_tag="ACHDLD_000265"
     CDS             complement(32539..33066)
                     /locus_tag="ACHDLD_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN3362029.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33296..34102
                     /gene="proB"
                     /locus_tag="ACHDLD_000266"
     CDS             33296..34102
                     /gene="proB"
                     /locus_tag="ACHDLD_000266"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN3362030.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34099..35346
                     /locus_tag="ACHDLD_000267"
     CDS             34099..35346
                     /locus_tag="ACHDLD_000267"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN3362031.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35422..35766
                     /locus_tag="ACHDLD_000268"
     CDS             35422..35766
                     /locus_tag="ACHDLD_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362032.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(35883..36209)
                     /locus_tag="ACHDLD_000269"
     CDS             complement(35883..36209)
                     /locus_tag="ACHDLD_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362033.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(36824..38026)
                     /locus_tag="ACHDLD_000270"
     CDS             complement(36824..38026)
                     /locus_tag="ACHDLD_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3362034.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38143..38658
                     /locus_tag="ACHDLD_000271"
     CDS             38143..38658
                     /locus_tag="ACHDLD_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3362035.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(38857..39303)
                     /locus_tag="ACHDLD_000272"
     CDS             complement(38857..39303)
                     /locus_tag="ACHDLD_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN3362036.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39350..40828)
                     /gene="msr(C)"
                     /locus_tag="ACHDLD_000273"
     CDS             complement(39350..40828)
                     /gene="msr(C)"
                     /locus_tag="ACHDLD_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN3362037.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41182..41811)
                     /locus_tag="ACHDLD_000274"
     CDS             complement(41182..41811)
                     /locus_tag="ACHDLD_000274"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3362038.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(41795..42259)
                     /locus_tag="ACHDLD_000275"
     CDS             complement(41795..42259)
                     /locus_tag="ACHDLD_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3362039.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(42746..44014)
                     /locus_tag="ACHDLD_000276"
     CDS             complement(42746..44014)
                     /locus_tag="ACHDLD_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3362040.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44245..44982)
                     /locus_tag="ACHDLD_000277"
     CDS             complement(44245..44982)
                     /locus_tag="ACHDLD_000277"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3362041.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(45049..45618)
                     /locus_tag="ACHDLD_000278"
     CDS             complement(45049..45618)
                     /locus_tag="ACHDLD_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN3362042.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            45746..46618
                     /locus_tag="ACHDLD_000279"
     CDS             45746..46618
                     /locus_tag="ACHDLD_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3362043.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            46960..47832
                     /locus_tag="ACHDLD_000280"
     CDS             46960..47832
                     /locus_tag="ACHDLD_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3362044.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            47829..49787
                     /gene="nagE"
                     /locus_tag="ACHDLD_000281"
     CDS             47829..49787
                     /gene="nagE"
                     /locus_tag="ACHDLD_000281"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN3362045.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT        14264 a        10730 c         9003 g        16072 t
ORIGIN      
        1 aaaaagaaac acctgttgaa aaaatcagtg tggaattatt gaaagaaatg cgtaatttga
       61 tgcaggaagg gatcattttt tcttttgatc aagttggttt gatcgatggt aaatatcgta
      121 tcccaatcta tgtgattgaa gattatattt ggaaattaaa aggcacccgg tatcgagaag
      181 aggaaatcat cgctacttta atctactctg tcaccgatcg acactggcaa ttggtagaag
      241 atgccgatct ttcttatctg ccattgaagt agatctattt cttatcaaaa aataaaaagg
      301 aaacaagaaa ctaggacatg attctgtctc aagtttcttg cttctttttt atttacagct
      361 cctgtcgttt atctggcaca aaaatcgaag gagtcgtttg ttggatccct aataaaacga
      421 tgatcgttac aatagctgta ctcaacccgc tggcaccatt cattacgaaa gaaaagagcc
      481 aaggattcat cccccatagt gcatacgctc cccagaaaac cactcctgca ataaaatgcc
      541 aaaaatatct ggaaagtgcg ccaatcaatg caccaaacac gatgcttttt ctaatttgtt
      601 tcgtattatc gtctacatag cctttttgga catttgaagc catgagccct gctgcgcctg
      661 caaaagtata ggcaatcggg tactcaatca acacttgaac gacacttaaa taataaactt
      721 gtcccaacgg aaaatgtagg atgccccaaa tgaaacctgc cagcatccct gcctttgtcc
      781 ctcttcttaa cgcaaaaaga gtcaatggaa tctgccctaa tgaaatagaa aaactgctgc
      841 cgatctggat cggaatcaac gacaacacca tcgctaacgc tgcaacaatc gttccttcta
      901 cccaaactct attttttccc ataaaaaaac ttcctccttt gaataaaaaa gtcacaaagg
      961 aggaagcagc tttcgaaaat agtcaaacag ctgtttattc acaattccta cgctcgcata
     1021 accgatacag gtacgaaggg tttagaaata gattcaatct cagcccaatt ggactcccct
     1081 ttgtgagttt atttattatt tattttttgt ttcaaacaat ggactaactg gtttgttttc
     1141 atggatacgt ttgattgcat ctcccattag tccgccaaca cttacttcat cgattttgtc
     1201 gattttacga tcttctggaa gatagatcga gtctgtaaca actagacgct cgatagctga
     1261 atcttctatt ctttgtaaag cagggccgga taatactgga tgtgtacatg aagcatacac
     1321 actagttgca ccagcttctt tcaaagcatt ggcagccaat gtgatcgtac ctgctgtatc
     1381 gatcatatca tcgatcagta cacaagtttt gcctttgact tgtccaatga tattcattac
     1441 ttctgcaaca tttgcttttg gacggcgttt gtctatgatc gcaatcggag atttcaaaaa
     1501 ttccgctaat ttacgtgccc gtgtgacacc accatggtct ggtgaaacaa cgacaacatc
     1561 atcgcctttg attccttttt ctaagaaata attggcaata agtggcgcac ccatcaaatg
     1621 atcgactgga atatcaaaga aaccttggat ttggactgca tgaaggtcca aagtcagcat
     1681 tcttgttgca ccagcttttt gaatcatgtt tgctactaat ttcgctgtga ttggttcccg
     1741 tgcacgtgct ttacggtctt gacgcgcata gccataataa ggcatgacta cgttgattgt
     1801 ttttgcactt gctcgtttca gtgcgtcaat catgatcagc aactccatca agttatcatt
     1861 gactgggctg ctcgttgatt gaataacata gacatgcgat ccacgaatac tttcttcaat
     1921 gtttacttga atttctccat cactaaattg agtaacagat gattttccta actcgacgcc
     1981 gactgctgct gcaattttct ctgctaatgg gcgattggaa tttaacgcaa aaatttttaa
     2041 tcttgggtca aagtaatgtt ttgacatggg gacctccact ttctttttca caactctaga
     2101 attctaccta ataaatagta gtcatatttt ttaaataaat caagtgattt tgcaaaagtc
     2161 ttagtttaga taagggagtt tttttgcata tccttcttta ttgacttgtc ttgctctcgc
     2221 aattgccagc gcatattctg gaatattgtc tgtaattgtt gacccggcag caatcgaact
     2281 gtttttagct acctcgacag gtccgatgat attcgtgcta gaaccaataa agctgtggtc
     2341 gccgacagtc gtatgatgtt tgttctttcc atcatagttg acgaagacaa caccacatcc
     2401 gacattgatt tcttctccca gcgtagcatc accaacataa gtcaagtgac cgactttagt
     2461 tcctttgccg atttgagcat ttttcacttc cacaaagttt ccgatatgga cgttttcgcc
     2521 aatttcagct tttggtcgta aatgtgcata aggtcccaca tctgcatgtt tcttgacatg
     2581 gctgctttca atgactgagt tttcaatgac gacgtgatcc tcaatcctac tgtctacgat
     2641 ttttgaatgt gacccgatga cacaatcaga accaatgact gtatttcctt ggatttgtac
     2701 acctgcttcg atcaatgtgt ctggaccgat ttctacgcct gcatcgatgt atgtcgttgc
     2761 tgaatcgaca aagcttacgc cattgaccat atgcatctgg ttaattcgct ttctcatgat
     2821 ctcatttgct ttagctaacg cgatccgatc attgactccc attgattctt caaaatcatc
     2881 cgtttgataa gcagcaactg tatggccttc ttcttttaag atttcgatga tatctgttag
     2941 atagtattca ccttgagcat tgtttgttcc aactttgctt aacgcatcga acaaagcttc
     3001 gttgtcaaaa cagtaagttc ctgtattgat ttcttgtaca agagcttctt ctggtgttgc
     3061 atctttttgt tccacgattt tttctacgat cccaatatgg tcacggatga ttcgtccata
     3121 tcctgttggg ttttctgctt gtgccgtcaa gatcgttgca cttgcatttt ttccttggtg
     3181 atattcaaaa agattgttca gcgtttcagt agtcaataat ggtgtatcgc cactgataac
     3241 taacgttgtt cctttttttc cttccaagaa agaagcagct tgcaaaacag catgtcctgt
     3301 tcccaattgt tctgcttgta acgcatattt actgcgttct cctagttgtg ctttaacttg
     3361 ttctgctccg tgtccaacga tcgtaatcac ttcatctggt ttcgtttcac tgacccgatt
     3421 aatgatatgt tctaccatag gtttaccaga aacgggatgt aaaactttat ataattttga
     3481 cttcatgcgg gtacctttcc ctgccgccaa aataattgca taacgtgcgt ccaactgcgt
     3541 cactcctaaa tatagtttgc tgatttcagt ttatcccagt atacccattt tttcaacaaa
     3601 acaagtgatt ttcttttatc ccagcgtttt ctggataaaa aaatagatag accataacgg
     3661 gcctatctat ttggtaaccg gtgctctcct gccaactcct cggtcacaag tcacactact
     3721 cataaaacat gaaaagctgt tttttgaaat cgtttcttgt gacttagagt taaacggtag
     3781 gcttacaacc tcttcattaa acgtttttca atcagctgac cttcggtatt agctcaataa
     3841 acggcaaaat atgaggagca atttttccgt ttattgagct aatactcagg acaggacgct
     3901 gatttcacaa cctctagata aacggtgttc aaaagccagt ttctcggtat taagccaaat
     3961 ttatgaaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg aaactaagcg
     4021 gctttttcac aacctcttat tctgaaaaat aatttcctgg cacaaccgta atatttttcg
     4081 tttgagtatc tacatctttg acataaagca gtgacgtata atcatcgatg gcgcgatgac
     4141 cgttgaattt tgattcggca aatacagtga tgccaacaag ttctgattcg aattcttcaa
     4201 tcaagctctt cattccgttg actgttccgc cgcctttcat gaaatcatcc actacaagga
     4261 cacgtgaacc acgtttcaaa cttctcttag acagttccat cttttcaatt ctttcagaag
     4321 atccagacac atagttgaca cttactgttg atccttctgt tatttttgag tcccggcgta
     4381 caataacgaa tggcgcattc agataatagg acacagcttg ggcaataggt acgccttttg
     4441 tggctacagt cattacagca tcgatttttt caccaaggta tttagaagca atgattcttc
     4501 caacttgacg cagcagttca ggttctccta acaaatcaga gagataaaca tatcctcctg
     4561 gaagcagacg gtcttgttct gatagacgtt ctgctagaga ttcaatatat tttttcgcct
     4621 cttcatatga aatttcaggg atgaacaata caccacctgc agcacccggg attgtttcca
     4681 aggttccata ccctcgttct ttaaatgttt tcttgatgat tcccaaatct tcactgatag
     4741 aagatttagc agattcataa cgttcggcaa agcttgttaa ggaaatcaat tcatgcgggt
     4801 gattcaataa atactgtgtc atgtcaacta gacgttcact gcgacgtact ttcacctatt
     4861 ccaactcctt ttaagaaatt ttatcgtttt acattatata agtttttctt caaaaattcg
     4921 agcattttat gaaaaaataa gaaaatagtt cggtatttat taaaataaaa agaagcctat
     4981 aaaaaatttt tcgtttttat aaggcttctt ttctttataa tttagttatt ttttcttctg
     5041 aaggcaacca atattcgttt gatgatattt acaatcagga acaaagcaat aaagactaat
     5101 gtgattgttg ctcctggagg tgtatctaga taaaacgagc tagttagtcc tgacagcatc
     5161 cctagaaatc ctacacaaac actgatagca atcacgacat tgaagctttt gcctaatcgc
     5221 ataccgattg ctgcgggaag aaccatgatt gcggatatca aaagtgcacc ggcaatcggg
     5281 atcatcacag caatagccac accagtaatc acattgaaca tcatcgacat caaatgaacg
     5341 ggtagcccat ccacgtgggc ggtatcttca tcaaaagtca gaacatacat cggccgtttg
     5401 aataaaaaga acaatatacc gatggcaacg aataagattc ctaaaaagat gacttgctcc
     5461 catgtaatgg tcacgatcga accaaacaga taggattgga tacttgttgc tgaattccct
     5521 ccgctcaaat tcatcaatac taacgctagc gccaaaccac cagccattaa aatggcaatg
     5581 gagatttctg aataggttcg gtagatcgtt cgcagatact ccaatatcac agctgctaat
     5641 acaacgacta gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc taacgctacc
     5701 cctgctaaag agacatgaga aagcgtatca gccatcagcg attgtcttct gatcactaaa
     5761 aacacaccta gcatcggggc gattccggca atgaagatag cggcaagaaa cgctcttctc
     5821 atgaattcat atgaaagcaa cgccattgag aatcctcctt tctcaccagt cggatctgtc
     5881 tgtccgcata ctgtttgata tcttcatgat catgggtgat cattagaact gatttcccat
     5941 ggacatgcgc actatgtcgg agaagacgat aaaattcatt tctggattct tcatccatac
     6001 ctgttgttgg ttcatcaaga acaaataagt cagggtcagt agcaaaaata cgcgctaaac
     6061 tgattcgctg cttttgtccg ccagacaatt cacctattcg ccgatttcgc atatcccaca
     6121 tccctacagc ttctaaagca cgctgtacat gttgatgatc tttttcagag agacgcttga
     6181 accagcggcc tcttggatat cttcctgaac ggactaattc gatcaccgta ctaggaaaac
     6241 ctgcattgaa agatgcaact tgttgaggaa tataaccgat acttaatttt tttccttctt
     6301 gatttttctc agcaatcttt atcattcctt ttgaaggttt cagtaatcct aacgatgctt
     6361 tcaccaaggt tgacttggct gctccatttt ctccagttaa aatgacaaat tcacccgcat
     6421 ctacatgata agagacatct tctagtacag gttcatcatc atagtaaaag gtcaaatcag
     6481 atatttcgat ataacgcacc ttacaacctc cattctaaaa cttaattgat actttctttt
     6541 aaagctttca gatttttttc catgacagaa atataatttt cgcctgcttt gatttgttca
     6601 ttagtcaagc tttctaaagg attcaacact tctagcttca ctcctgtttc attcgacaat
     6661 gtctcagcta ccttcgaaga ggcattttct tcaaaataaa tcactttgat gttgtttttc
     6721 ttcacaaatt cttttagctc agctaagcga cttggtgtgg gttcctgatc tggtgaaacg
     6781 cccgaaatcg ctacttgttt taagtcgtat tgattcgcta aataagcaaa agcagcatgc
     6841 tgagtgacaa atgtgcggtt tgttgcatct tttagctcat cggagtattt ttgatccagt
     6901 gcttcaagct ttttgatata agcatcggca ttcttcgtaa aagtttcctt tttatctgga
     6961 tatttttcgc ctaattcttc agcaatcgtt ctgacttctt ttatagctaa taccggatcg
     7021 agccaaacat gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt
     7081 tcgtctgctt cttgcgattc taaacggtcg attcctttag ctgcttcgat catcaaggtc
     7141 tgtttgctat ccactgaatc ctttaacgaa tccacaaaaa attccatgtc tgaactatta
     7201 tacacgaaaa catctgcatc ggaaatttct gctctttctt ttgcacttgg ttcgaaatca
     7261 tgcggctccg ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc tacgatctct
     7321 tttgtaaaat catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct
     7381 gcttccttgt tcgtatttcc acatgctgca aacagtaaag cacctatcat taccgtagct
     7441 gtcaaaagat attttttcat ttatatgctc ctttcaatga acaaatcgga attttacgat
     7501 ttattaacgt gaactaatgt atcaaaataa aaaaacacag tcaatagaaa tccatcaatt
     7561 tctgatttaa gcatgtatat tcttgattaa atcgtaaaat ggatgagttg ctgctcatat
     7621 ctagccattt cactacaata aaaaactgaa gaataccatt ttaggattcc tcagtttttc
     7681 atcacctatt ttacttctta tattttattg cagggttcgt actaaataaa cttcctcaca
     7741 aaaacctttt aacccattat atactctctg cgctcttgaa taatgttgac acaaggcaaa
     7801 aacggttggt ccgcttccac tcatcaatgc agcatctgct ccgtacttga tcatacgctc
     7861 tttgatttga tggatgactg gatgccgctt tcccgttata tcttctaaac tgtttcccat
     7921 ttttttgatc atctgctgat aatcttgctc tcggatggct tcagcaagtc cgtggatgtc
     7981 ttgatgttgg atggcttgcg tatctacttc tctaaacact gtacgtgtag agacactcat
     8041 tcttggtttg accaacacaa cccagcactg tggcatagaa ggtaatactt ggatttcttc
     8101 ccctttgcca ccgacaaatg ccgtacttcc gtagacacag taaggaacat ctgtaccgac
     8161 ttccaaaccg atttctgcca gttcttctaa cgaacagccc agattccata attggttgat
     8221 ccctcttaga gcagctgcac agtctgtact tcctccgcct aatccagctg ctactggaat
     8281 attcttctta acaaagatct tgacaccggt tttgatattg tatttatttt ttagcaataa
     8341 agcagcttga taaacattgt tccgttcatc taccggtaaa aatgctttat ttgtttctac
     8401 aatgattttg tcttctgtta tttcttcaaa aaatagatga tctgccaagt ctacgctcga
     8461 catcaccatt tccaactcat gatacccatc ttgccgtcta tataatacat ccagtcctaa
     8521 atttatcttg gctggcgctt tttcaatcaa ttccatcctc tcacccattc gaaaagtcta
     8581 agtcgcaagt attctatcat agggaaaaat aaaagtatag cttatgtctg cctagtggtt
     8641 gattttgtca tcgtttcaac agctttcaca gaattttctc ttcataatcc aaacaagttc
     8701 tagtgattct tataattctg tgagcacttc ttctgccaaa ctcggaacat ttcctttttc
     8761 ttttcctatc agactgagta gctgcatggt agcaaatccg aaattacttc ctttcctata
     8821 agcaaaataa cgtccttccc aatgatcgac atattttaat ttcttttcat aagcagactg
     8881 gaaggaatag aaaaaattac cataattata tacgatatgc atgacttttt cttgaaagaa
     8941 agaaaaatct gtttctccaa cattagctaa tggagctaag cctagattgg cttccgagta
     9001 gcccattttc tcgtatacaa taaacagatt ggctaacaga tagtcagcta attcctcaga
     9061 ttctttaggt aagacccgca acaaatcata tcccgcctgc ttcttcgtca tcggctgagc
     9121 ggtgatgaag ccaacgactt tttgatcttt acggacaata ccgacaccgc tagataatac
     9181 atatgattta tcaaatcgtc ccacagagaa gtatttttct cgtgcacctt gaagccattt
     9241 ttgtgaaact tcatttacag cttgatgtaa ttcttccggc agttgatcgt accaagtaaa
     9301 atggtatcct tctttcatca aacgttgata ttcaagttgc tgactcagcc gagacaagtc
     9361 ttctgttgct agatcaacaa tccctgcttc acctactttc gtgaagtgaa aacccaattc
     9421 atgaagcatc actacgtagc tttctgatat cttataaaac gcaagctgat agcctaaaca
     9481 atctgcttgt ttcataaact gattcgttgc tgcccgcatc attttaggat tgccaactgg
     9541 atctcctaaa acaaagcatt tgtttccttt gatctgataa ccaaaaacga cttgatcttg
     9601 gtcgtcttct tggtaataaa aataactgta acctgacaag cgaagctgat ggcttgtagc
     9661 tgtgcctccg tatgtttcca ataaacggtc aaaacggtct ttttcgtact cactgccaag
     9721 cttcgtagta tcttcagcta aatattgata aagcgccact aaagctagca atgagatgcc
     9781 caaaccaata agccctgaga accacacatc atctgaagga aacaaaataa aatgatttcc
     9841 tgttattcga ccgactaaaa agcgtccgct gtgatagcct gcaattgcgt aaacgataaa
     9901 caaaaagccg aacaagctag catcaaagat tatagctccc caagaataaa cgaattttgt
     9961 tcgataaaat tcttttctgg ataaccacac agctaacaga atcaagacat ataccaatat
    10021 caactgccaa gataacgtac gtgtgattgt atttaaaata ccaaaagata gtaaagcaat
    10081 cgtaggcaga aatgcctttt tgacttttac tgaaagtgct ctagcaaggc cgagtaataa
    10141 aaagccaacc aatagattca ctgtctgatc taaaaaattg aatgagaacg gcaataatac
    10201 ttgaaatagc cgactgattg cagacagatt agtgattgtt gatagaagta ccatcatgat
    10261 ccccgcaaaa tagacagcac ttacgagcac gatatgcgca accttttgga aaaacagtcg
    10321 cggaatgtta tctaaaaatc gattgatttt gactcctgta tgtgtgatga acaatagaag
    10381 tcccgtcata aaaggtagaa cataataaaa caaccgataa tataatagcc aaactactgc
    10441 tacatcttgc cgaacagaca attgactcat tcctaagatc atcaaaacgt caaaagtccc
    10501 cattccacct gggaccatcg tcagcattcc tatcaatgta gcaatcatga acataggata
    10561 gatcgaaagc atcgaaatat ctattctcat caacttgccg atgatcaaaa ataccgcaag
    10621 ggctcccgtc cattgtccaa aggatgccag tagcagacca agcaagcctt ttggataaaa
    10681 ctcgaagaac aatgtctttc tctttagata agcaaaaaag aaaatgactg gtgctaaaaa
    10741 actaccgccg aaaagccaga tccaatagct tcgaaaaata ctttctgact gaataaaaaa
    10801 gacatcaata aatgtaagaa acgaccatac agacaaacca gtcagcataa atagtgcgac
    10861 ttttgataca gtggctaaaa ccatttttct atttgtctgc tttccataaa aactagcccg
    10921 cagacttgct cctaccacac caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca
    10981 cgcagcaaga agaaactctt ttctttccat ttttggtttt ccttgttttt ctaagactct
    11041 gatcgtcacc caatcataca acaacatcgg agtgacaccc agcagtccag ccagaatcat
    11101 gagcaggatc gtcatgcggc tttgttgtcc catcctttgc caaatatctt gccaactcat
    11161 gccattagca atatttgcga cttgatttgc cacaaagact aacacggagc cgaaaaaaat
    11221 cagttttaaa atcagactat gttcctttat ccattgcagc atcaagtatc gtttattttt
    11281 cacattttcc cattcctaac ttgtatactt ttgtgtcata ccccaatgtt atcttattta
    11341 ttttaatttt tccactttag acctgttctt tgcttgcgat gatagaaaaa gaaagatatc
    11401 ctaatgctaa aggagaggat aacatgaaag taggatttat cggcacaggg aatatggcac
    11461 aagctattat tgatggttta ttaaaaaaag gaacagtctc gaaagaagag gtgttcgttt
    11521 ccagtggaca ttttgaaaat gcacaaaaat ttgctgctga aactggcgtc catgcatgca
    11581 aaagtaatca ggaagttgca tcacaatcag atatcgttct tttagccgtg aaaccaaaaa
    11641 tcattgaaaa agtattggaa gaactaagag aatatagcca gaaagtctta ttcatttcta
    11701 ttgcttcagg aaaatcatta atagatttag aaacagcgct tgaagatcct caggcctcca
    11761 tcgtacgtgt gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca
    11821 aaaatgaagc tgccacggag gaacagcttc agcaagctac gatgattttt gaagcaatcg
    11881 gcagcgtcta tcctttagca gaatcagatt tcagtacatt cattggcctt gctggcagct
    11941 cacccgcatt cgtttacatg ctgatcgatg cgatgggacg gactggtgta ctgaatggtt
    12001 tgtccaaaca gacagccact gagattgctg caaaagcaat tatgggttct tgtcaaaaat
    12061 tcctttcaag tgataaaaat ccatgggaac ttgtagacca agtttcttct cctggcggta
    12121 caactgttgc aggtgtagtt gccttagaag aagcaggatt catcccagca atcatcaaag
    12181 gaatcaatac gacgatcgag aaagatcttg agatgttgaa taaaaaataa tgatagcaat
    12241 gttttttcgt gccttacaaa aaaagaagag ctgtggcttt tgccgcggct cttcttttgt
    12301 attttttatt atttagcata atcagttgca cgagtttccc gaataacgat tactttgatg
    12361 tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca
    12421 gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg tccagcttga
    12481 actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag acttcttaaa
    12541 cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc tgagagcgca
    12601 tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc atgatgagaa
    12661 gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc cgcaccgatc
    12721 tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg gagcaacccg
    12781 gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc agctaatttt
    12841 gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa tcgcagacgt
    12901 ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa cgctgcctct
    12961 tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac catttcttcg
    13021 atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt
    13081 tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc atcgatgatt
    13141 aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg tccaatgata
    13201 cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc tgaaacttga
    13261 tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg atctgcttct
    13321 tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt cagctcttct
    13381 tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc aattttttct
    13441 agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc ttcaattaat
    13501 tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg tttttctaac
    13561 gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc ttgagatttt
    13621 aattctaact tactttctct cagctcactt tcaacttcag aacggtattt ctgattttct
    13681 tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt tgcactatcg
    13741 atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg tgactttgcg
    13801 accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag aatgttgaat
    13861 accataaaat ccacctccat actatctttt tcatttttat catctataca gcttctatga
    13921 taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac
    13981 aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa gaaacggctt
    14041 tctacttatt cattaaacga tggtttttaa aataaatgac actagtttca tataggtaaa
    14101 cctttaaatt gagaaacttt tctattaaat tattctttgt agatgaacag atatttttat
    14161 tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat aaaaaaacgg
    14221 caggtcaagg agagtaccta ctcttcagac cagccgtttt tgtttatcat aatttttatt
    14281 cttctactaa aggcaattct tcttgtcctt tttcatcttc tgggacagcg acttcttcac
    14341 caataccata agctgctcta actagagcag aaacttctgc catcatttca ggatgattag
    14401 ccatatagat tttagcgttt tcccgtcctt gaccaatccg atcttcttta taagaatacc
    14461 aagcaccgct tttatcaaca atgtcttttt ctactgccat gtctagaagt tctccttctt
    14521 gtgagatccc taaaccatac atgacatcta cttcagcgat tttgaaagga ggtgctactt
    14581 tgttttttac gactttgatc tttgtacggt taccaacaat atctgtacct tgtttcaatt
    14641 gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag
    14701 gagtgatttc tggattccca aacatcacgc ctactttttc acggatttgg ttgatgaaaa
    14761 tagcgattgt ttttgttttg ttgatcgaac cagagagttt acgcaatgct tgagacatca
    14821 aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa
    14881 ctaaagcagc aactgaatcg actactacga tatctacagc cccacttgat actaaagcat
    14941 cagcgatctc tagaccttgt tctcctgtgt caggctgtga aagaagtagt tcatcgatat
    15001 tcacacctaa tttttgtgca tattgcggat ctaacgcatg ctcagcatca atgaaagcgg
    15061 ccgttccgcc atttttttgt acttctgcaa tagcgtgtag tgcaactgtt gttttacctg
    15121 aactttcagg tccatatact tcgatgatac gtccacgggg gtaaccgccg acacctaaag
    15181 cgacatctaa cgcaagagag ccacttggaa ttgtagagat ttgttggtcg attttttcgc
    15241 ccaatttcat aatagagcct ttaccatagc tcttttcaat ttttttcagt gcagcatcta
    15301 aggctgcttt acgatcatct gccaatacga taatcctcct tatatttaca aatcagagca
    15361 acatttcatt atccttatca tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa
    15421 atattcgcat ttatcatttt cttaaaagct gttttctcaa aagatcaaat ccttgcataa
    15481 ccgcattata tctgatagca gcacgatctc tggcaaagtg atatttttcc gaaaccacgc
    15541 cgtctttaga tgaaatagcg atccatactg ttcctgcaga ttgaccttct aacgtgtccg
    15601 gtccagccac tccggtgaaa gctacactgt aatctgttct tgctatctca cgtgcttgaa
    15661 tggccatctg ctccacacat tctttgctga ctgttccata ttctgctaat agctccggat
    15721 caatctccag aaaaccagct tttgtttgga gcgagtaggt aacgaatccg cctggaaata
    15781 cttcagatac accagcaatg tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt
    15841 ccgctgctgt tacagtctgc ttgttttctt tcagtagttc cactacgact tttgctagcg
    15901 aattatcatc gccgtatcca taaaaatatt cccctactcg ttcttggatt ttttcttcta
    15961 atgctaaaag agcttgattc cctgcctgaa catcatttgt tttcactgtc agtcgtaatg
    16021 tcacttcatt tggtttggca tatggtgcga tcgttggatt gatctgtgtc tcaatcagat
    16081 cttttagctc agtgaccagt cgcgattcac caatcccata aaagcgcaaa actttagaaa
    16141 ttagtttttc ttcagaagga aaacgttcct ctagtaaagg acgtacttgt tccacaaaca
    16201 ttggtttcaa ctcattaggc ggaccgggaa gaaggatata cgcatgtgta tctgtttgat
    16261 aaaaagtccc taaagcaagc cctgtacgat ttggtagagg aaccccgcct tcgataatct
    16321 gggactgttg cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata
    16381 attttttgta tccttccgta ttttgtatga gagacttacc gaggtgagcc gcagttgctt
    16441 cttttgtcag gtcatcttct gtcggtccta atccgccaca taagagtatg agttcacttc
    16501 ttgtttcagc tagagcaatc aactcttcta aacgctgctg attatctcct acaactgttt
    16561 gataataaac ttcgatacct agatcagcta gctgttctga caagaaagtg gcattcgtat
    16621 tcacgacttg tcctagtaaa agttctgttc cgacagctat tatttctgct ttcattactt
    16681 cccgctccct tatatagtat acgctgtatt tttatatttt tttatttaaa aagagggcaa
    16741 ggagctgtga caaaaatctt gtcacagctc cttgttccaa ataaacagtg ttctcctgcc
    16801 aaaacctcgg tcataagtca caatatacat aaaacatgaa aagctgtttt ttgaaattat
    16861 cccttgtgac tcagagttag acggcaggct cacaacctct ccactaaacg atacttacat
    16921 tgatcctttg aacacaccgc tgtttttgat gaagtaatcc acacctgaat agatagtaaa
    16981 gatcagacaa gcataaagca tgatttgatc tactggaata ccgatcaggc tgaatgggat
    17041 attattgata aacagcaaga taatagcaac catttgtgta gccgtcttga cttttcccgg
    17101 ccaagctgca gccatgactt ctccatgttc gacaagtagt aaacgcaagc ctgtgacggc
    17161 taattcacga cagacaatga tcgctactac ccaaccaggg gcctttcctt ggtcaaccaa
    17221 catgataaaa gcagtcataa ctagcatttt atctgccaac gggtccgcaa acttgccaaa
    17281 attagtgaca aggcctcgcg cacgggcaat ttttccatct aaccagtctg tgatactagc
    17341 taaagcaaaa ataactgcac ctactagctg tgtgacagcc agtgtggtgt ctcctacgtt
    17401 aaattgtccc caatctaaag gtaaacctac aaccaaaatg aaaatcggga tcatacaaat
    17461 tctaagtaca gttaatttat ttggtaaatt cacactatcg actcctttct tccctattct
    17521 acgtgactat cagcgaagtt tccaccgata ttatttactt ttccaaatga atttattcaa
    17581 aggagtattt ttattgaaat tgatcttttt gaattaacga agaaggaccg cgacacagat
    17641 ttgtcacgat ccttcttttt tatacctaca agtaaacaga tgcgtaaact aatttatacg
    17701 atcattctgc gtattgaaga gtaaggttca cattttttgg actctgattg ttttcccctg
    17761 gattaactgg tacggattct ccattcgctt tgatcgttgc attgcttgct gcaccaatcg
    17821 tgatggtcgc atttgttgct gtttccggta acacggtact ctgtgtttca tttgctgcca
    17881 gtgtcccttg ataaacatag gcattgtcta ctagcacacc taaccataca cgattgacag
    17941 cagtaaattc aagggttaca ggcttggtag cgtctgtgat atttactgcg atggcactgc
    18001 cagaatcttc tcctgtagct attgccattt tcttttcttc acttgatttt gttgttgatt
    18061 ctactgtaga agactctttg gtctgcgtgc tttgctctga ggaagaggac tcctcgactg
    18121 atccttctac tttaagcgaa gaagctgtgt ctccgattat tggatcagct tgacgatctt
    18181 gccaagtcat atagcccacg acaacgacaa tcgccaaagc aaccaatcct aataaaatga
    18241 caggaagact tgtccaaaag cgacttggat gcgattcttc ctgatgcatt gcttttctag
    18301 aagtacttac tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat ttgcttttcc
    18361 catcaaaaac atcgactaaa tggtctccat cttctcctac agcttcagcg tattgccgaa
    18421 taaatgcccg cacgtaaaat ttaccaggca attggtcaaa atcatcattg tcgattgctt
    18481 ccaaataacg tttttgaatt tttgtaatct gctgtaattc atctaaagat atttttttat
    18541 tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa agccactcgt tcatcttctc
    18601 cagtctgttt gattctccga agcatctgct gttgcaacga aagacgttat gtttgggtgc
    18661 tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa acattgttct cctgccaact
    18721 cctcggtcac aagtcacaat atccaataaa tataagaagc tgttttttga acttgttcct
    18781 tgtgactcgg agttaaacgg caggctcacg acctctttct taaacggtgt tcaatcagct
    18841 gttcctcggt attagctcaa aaaccggcaa aatataagga gatatttttc cgtatgtttc
    18901 tctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt gttcaaaagc
    18961 ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt aagaagctgt ttttacttgg
    19021 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttggtaca cggtgttcca
    19081 gaagtagctt cttcggcaat aagccgaaat atcacaaaaa tttgagaagc aattttcgta
    19141 aattccttct tatttcttga agctgaccac ttctgtcaca acctctttga ttacttcgtt
    19201 tgattttatt ttaatataag ttgattaaaa gagagcatga tttgttttat tttctcacta
    19261 atttttccaa cacaactaag aataccataa aagctgattt tggaggaggt tcctactaca
    19321 agtctaaaac gaaatttaag acttttcctc ataaaatact ggtttatttt ggtctcatgt
    19381 aaaactcgct gaaggcctct tcacttataa atgcttcccc agctgccagt acgtctttca
    19441 tttggatcga atcgatgatt tcaggaagat caaataaagt tcgatcgcca aataaacttt
    19501 gcgtaaattg attggcaata tattcgatcg aatttaatga ttggaaatat tgtccaagca
    19561 ttttcttctt cagcaaatcg agatttgttt ctgacacttc cggatcatca gcgaatccaa
    19621 gaatgatttc tttgactttt tctgctgctt tctctggttc gtccgtatct ccgctgaagt
    19681 ccgcaaagtg aaattcacga tccaagctga attcaaaacc aaagctgtca tcgataattc
    19741 cttgattgta catcgctaga tagtttcgag aagtattgcc taaaatcatt tggaacaaca
    19801 gattgattgc tgttttatag cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc
    19861 ctttaatacc aagtacaaat ttatctcgtg taatggctgc ttccaatgca ctttgtttga
    19921 tgatttcttt tgtgttttca gggaaataac gtacaatttc ttgctttgga gggaaatttt
    19981 ttgcttcttg gttttcccga ataagtttca tcaatttctc aggctccatc ttaccaacta
    20041 caaataaaac catattgctt ggctgataaa acgtccgata gcacgtatac aagtcttgag
    20101 ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag
    20161 gatacaagtt atttagtatg ccaaaaaaca tccgccagtt agggtcgtct tcatacattt
    20221 ggatttcctg accgatgatt cctttttcct tgtttacagt ttcttctgtg aagtaaggtg
    20281 cttgtacaaa atcgatcaga gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca
    20341 gatagctggt tttcgtaaaa cttgtgaaag cattggcgga agctccttgt ttcccaaata
    20401 attgaaagac atcaccatct tctttttcaa acaacttatg ttctaaaaaa tgggcaattc
    20461 catctggaac tttcaccttt tctttttcac catagggaat aaattcgtta tcgatcgaac
    20521 cataattcgt actgaacaaa ccatatgtct tgtgatagtc atttttaggc aacaaataca
    20581 cggtcaaacc gtttggcagt acttcatgat aaagtgtttc attgatttgc tcatattctg
    20641 ttttattcat ttgcatttcc tcctgctaaa aagaagattg cctgcaattc gatttgctca
    20701 gctacttgtt gtatctcttt gatcgttacg ctctccatac gtttcaacca ttcttcatca
    20761 gataattttg tttccggcag ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga
    20821 gagtctagag aaagcagata ttggtttctc agcattgctt ttgtctgaga cagttccaac
    20881 tctgttattt caccatttcg caaactttct aattgctcgt gaatcagatg aagtacttga
    20941 tttcggttct tttcatcgat accggtttgg acactcatga atcctcggaa cgtatccacg
    21001 ctactagagg cataataagc tagactttct ttctctctga cattcatgaa aagtttagag
    21061 tgaggaaaac cgccaaataa accgttaaag accattagcg caaaacgttc tggttcatcg
    21121 taatacacat ttgtctgata agctaggttc agctttgctt gaacgattgg ctcacgtact
    21181 tgtcgttcct tcacgatatt cacttgaggt tgggtataaa acagatctgg ataaactgtt
    21241 tcccgcgact caaatggtaa ttgcccaact gcttcagctg ctttgtccgg atcaacatcg
    21301 ccgacaacaa aaatgtcgat ttgatcattt gccatcattt cttgatagta ggacgctagc
    21361 gaactagcag tgatcttgtc caaatcttcg actacgccca accctgggat cttttgttcc
    21421 gggctgtttt caaaataaag ttcttgcaag gcataggaag caaaagtttg tttatcttcc
    21481 ttcaaacttt ccaaatatag acgcagattg ttcttttcca agtcaaacgt caactgatca
    21541 aactgatgat ttttaatatt tggataaaat aaaacctctt ttaaaaaatc taccgcttga
    21601 gaaaaaaggt ttggatcatt gacatattca ccattcacaa aactgatccc tgcgtttagc
    21661 caatgtaaat ttcctttttt tctaactgat aatccaaagc ttgcgccata aagctctgca
    21721 agttgcgcac ttaactttgt ctgatccgaa taattcaaac tatttgtttc caacatgctg
    21781 ctgagaagtg aacgtttcgt gatgacttct tgttgcagac gcgcagtaaa acggataaaa
    21841 atccggatcg ttttgtattg ttttgttggt aagacatgaa gattcacgcc ttctgccaat
    21901 tgatacacca tatttatatg ctccttttca cagttgattg cccgcttact cttttaatgg
    21961 gtcaacctgc cttttttact tattcctctg cttgaaattt ataagttcag gcaattcgcc
    22021 gttttttcta aatgatgtta cactttatag tatagcatag atgtttttta tcaaaagaaa
    22081 cagacggcta aaagcgtata ttgttttttt attttttatt tttcgatata ctaaagacaa
    22141 ataaaagaag ggagggtcat tcatgatcaa agaattcaaa gagttcatca tgcgtggaga
    22201 tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg tttacaagca tcgtaaacca
    22261 agtggtgcaa ggcttgatca caccactaat aggttggttt gtcgcaatga tcacaggtac
    22321 ttctgattta gaaggagcac tttcaattct tgactggtcg ccgacaaaag gcgtcacatt
    22381 tgcgtttggt aacgttatta gtgctatcat tacatttctg atcactggtt ttgtcttatt
    22441 cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag agcaagtgga
    22501 agtggaagaa actcctactg cagaagatta tcttagtgac atacgcgatc ttcttgccaa
    22561 acaagtggca gaagacaata cacatccagt agagcaaaca aatctttccg atacttctaa
    22621 ataataaaga atctaaaaaa aaagaaagcc gattttcagt gtcggctttc tttttttaga
    22681 ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat cttccaaata
    22741 cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt attcttgtgc
    22801 gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg tttcttgaac
    22861 aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac gtccaaaatg
    22921 gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat cagtatcttg
    22981 aaaaaacgta tgccaagctt ctccatcaat gtcatcaact tcttgataag gacactgttg
    23041 ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa
    23101 ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt ttttattctt
    23161 agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct
    23221 ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag
    23281 ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa
    23341 ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca
    23401 accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt
    23461 gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt
    23521 taccataaaa aaaaaaagaa atgcaataaa aataaataag aagctcatta agatttttct
    23581 aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta ctctcccgta
    23641 agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta caaaatcaac
    23701 gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga tttcgcaatt
    23761 ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca atgtgatctc
    23821 tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt catctttttg
    23881 atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat tgcttataac
    23941 gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc tcaatgcctg
    24001 atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc ttaaataata
    24061 ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag caaatgcttc
    24121 ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat tcctccataa
    24181 attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga acgatatgtt
    24241 atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac tgctcttcat
    24301 tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct tgggctattg
    24361 ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt aatgctcctt
    24421 gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac
    24481 ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg tcttctttcg
    24541 taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga actagttggg
    24601 catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat aaacgatgta
    24661 aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca agttccggcc
    24721 acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg gtctactccc
    24781 cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg gcatttaagc
    24841 tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt ttcaatcctg
    24901 gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg gcattccatt
    24961 tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt tctttgagct
    25021 gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg actgccccag
    25081 tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt atgatcacac
    25141 catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga
    25201 tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt tcaatcagtt
    25261 ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga tgcacgccgt
    25321 gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca gcttgttcac
    25381 gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga agaaagagtt
    25441 tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag acgaaattat
    25501 cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt tgctttttat
    25561 ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc
    25621 tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa ttcttccatc
    25681 cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc tgttgcgacc
    25741 cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt tcttcctctg
    25801 cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa
    25861 aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt attgggtttt
    25921 gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc atctccaaca
    25981 taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt ctccatcggg
    26041 ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca cgaatttcgt
    26101 cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa
    26161 tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa agccattgcg
    26221 agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg tctagataag
    26281 tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca ttaaaatcat
    26341 cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt ttatccgcat
    26401 catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca aacgtattct
    26461 tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag cggatcggac
    26521 gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg tttttgatca
    26581 tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg tcttcgccaa
    26641 tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt ttcgcttcac
    26701 tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca ccatggatat
    26761 cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc caaccgggac
    26821 gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt ttccataaag
    26881 cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga cttgctccga
    26941 cttccagttc atcgatggat tggtcactta atttcccata attttcaaat ttacgggtac
    27001 ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac acttgaataa
    27061 atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca ggttcgacat
    27121 tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact tctggtgctg
    27181 taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg aaattcgata
    27241 cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg
    27301 tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag acatacatac
    27361 cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat gtattgtaga
    27421 tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt ttgctggtac
    27481 tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg caccgatttt
    27541 ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc
    27601 ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta atgttacgcc
    27661 atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga ttcccattcc
    27721 atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa caccagttag
    27781 gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct gatataaaaa
    27841 atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc
    27901 tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg taaaccaact
    27961 catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct tataaagaaa
    28021 agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg ttccactttt
    28081 cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt ggctactttg
    28141 tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc ccagaggcat
    28201 ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa tgatcagctg
    28261 cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg ctaatgattt
    28321 ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt gtcctgatac
    28381 agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt tttgaacagc
    28441 tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta gctgtgcttt
    28501 gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg ctgcttcatt
    28561 caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg cagcatagct
    28621 catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct tttcatctgg
    28681 gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag taaatgcatc
    28741 tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg catcgaaagc
    28801 tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt ggtgttcgaa
    28861 gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga acattgcatc
    28921 tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt cgtcacgttt
    28981 gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga aggttggtgg
    29041 tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa tatggtcatc
    29101 tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag caaaattata
    29161 agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat cggattcgaa
    29221 cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag gcacacggaa
    29281 acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt
    29341 cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc
    29401 ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa gcagctgatc
    29461 aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt attctccagg
    29521 attttcagga gattcatccc aattcattcc caaccaagca aggttttcca actggctttt
    29581 ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa taaagtctcc
    29641 atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc caatatgcag
    29701 gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct tcctcttctt
    29761 tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc aaatatcatt
    29821 tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa agccaactgg
    29881 ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta gtctttttct
    29941 ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca aagatcatgc
    30001 gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca ttcatgtaat
    30061 gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact ccttgttggc
    30121 gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg acgactggtt
    30181 ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat tcagagactt
    30241 tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc aatttgatca
    30301 atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg acagatatac
    30361 cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc acacgtttca
    30421 aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta gggatcatca
    30481 aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca atgatcacgc
    30541 ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct aacagttgac
    30601 tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca aaaactttct
    30661 tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag atgatcatca
    30721 gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga ggggtcgaga
    30781 tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga agataagtca
    30841 agctgacctc acttagtttt tgttctactt gtttgataat agctgagata tgtttggcca
    30901 acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta aatgtattat
    30961 tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa aatgaaatac
    31021 ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc atcgtttttc
    31081 ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt tcagccaaag
    31141 ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag ttgtttttag
    31201 gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt tcaatatgac
    31261 tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc gttggctgtg
    31321 ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca atcgctggtt
    31381 catttaattt taccccacct gcagctttta agtaagcatc ttgattctga agaagcaacc
    31441 cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca agacctgttg
    31501 ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc tctaccaaaa
    31561 ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg tctaggcgtt
    31621 cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca tgcatttcaa
    31681 aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa atccgaaaac
    31741 tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct aacattcttg
    31801 ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct atcccatttg
    31861 ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca cttcctgcca
    31921 cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca taatcagggg
    31981 agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat agataaaatt
    32041 ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc
    32101 cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact tgtaacagca
    32161 aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct ggcaccacac
    32221 ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt tcttttttag
    32281 ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt gtccgagttc
    32341 tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt ccgcaatttg
    32401 ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca aattgtactt
    32461 ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat taaatcaagc
    32521 ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac gttgagcagt
    32581 cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc
    32641 ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga tatggccttc
    32701 atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg tcaacactaa
    32761 aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat cttctcccat
    32821 gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat tttctgatac
    32881 atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg ctttccagat
    32941 acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc
    33001 gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa atcctctttt
    33061 tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg aggctgtaaa
    33121 agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc attaattcgt
    33181 tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga ctttactcgt
    33241 caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt gtcacatgcg
    33301 taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta cattgattta
    33361 tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc tttctgatct
    33421 tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg ttggtatgca
    33481 taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg cggcggctgt
    33541 tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata atcaacagac
    33601 ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta aaaatgtgag
    33661 gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg aaaacgatac
    33721 tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc agctgtcagc
    33781 catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata tcgacgggtt
    33841 ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata tcgataaaat
    33901 cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa caggcggtat
    33961 gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa tgattttagc
    34021 aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag taggtacttt
    34081 atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc aaaagatagc
    34141 gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt tcaaatggca
    34201 caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga cttagaaaaa
    34261 gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac acctgagcga
    34321 atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa
    34381 gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta
    34441 ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc agctagtcta
    34501 gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt cttctcgaat
    34561 caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc
    34621 atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg attgacagaa
    34681 tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt caaagaacag
    34741 gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat cgataaagaa
    34801 gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc
    34861 tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt cttgccagct
    34921 attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg tgcgttagaa
    34981 tatctagaga cggctgcttt agcagaagac acagattggg atactgaata tctagattat
    35041 atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat caatcggcat
    35101 aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca acgtttctta
    35161 aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac agacggatcg
    35221 gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc ccgtggtcca
    35281 atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg acaaatccgc
    35341 gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt aaaggttcta
    35401 tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat ttaccttatt
    35461 tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt tcaattcaaa
    35521 aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa
    35581 aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt attagcgaca
    35641 atcaattact attctttttt cgatcattca atcgcttata cgattacgct actgattgct
    35701 gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa tcggactttc
    35761 cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa aactgccgga
    35821 ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata aacggcgttc
    35881 gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat
    35941 gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt tttttctttt
    36001 tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat accagctatt
    36061 ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa ttctttgata
    36121 tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac gagtgtcaac
    36181 ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt tcttaaatga
    36241 attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa aagggaatgt
    36301 gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg ccaactcctc
    36361 aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt gttccttgtg
    36421 attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat cagctgaccc
    36481 tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt tgtttctcta
    36541 atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa tcagctgtcc
    36601 ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta
    36661 atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca aaagcttgca
    36721 tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc
    36781 ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct tcttcatttg
    36841 tagcaaaata attacggtga taaatgatac aacatacact gataagaaca atggcacctg
    36901 caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca ataaatggtg
    36961 ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg tttcgttgca
    37021 gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct tggaaaaatc
    37081 ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct cctaataaca
    37141 gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc
    37201 gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc cagccaaaaa
    37261 atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca tacgcaagta
    37321 aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga agtttatgat
    37381 tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc tcagtttcag
    37441 gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg
    37501 gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca gccattccgc
    37561 agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact aaaatactga
    37621 cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag ccggcacgta
    37681 acccaatcag cattccgata ttcccagata atccgccaag accagcgaat acaacgatga
    37741 taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag aaacctgtaa
    37801 atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc gtttcagctg
    37861 gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga cccactccca
    37921 taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc tttgtttctt
    37981 catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct ttctctctat
    38041 tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta
    38101 ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac tttagcctat
    38161 atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa gcagctgatc
    38221 acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa gttagaccag
    38281 cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat ctaccacttg
    38341 actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc cgctcctacg
    38401 caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa aacaacagct
    38461 actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa
    38521 aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt tgtgatccag
    38581 cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag agaacaaatg
    38641 cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat tgaagagcaa
    38701 attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga acgacaggcc
    38761 aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact atgacaaaaa
    38821 gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt gaattgcttt
    38881 tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag cggcaatcac
    38941 attcattttc caagcattca tataagcaga gaataattga tcaggtattc cttgttccat
    39001 gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc atgaaatagt
    39061 taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa tttttttcgc
    39121 cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag gcactagacc
    39181 aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg cgctcatccc
    39241 caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt tatttgtagg
    39301 caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt taaaaatttc
    39361 tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca gccgtacgct
    39421 cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt tgcattagct
    39481 tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct aacagcaaca
    39541 cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct cccccactta
    39601 atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc agcaaactac
    39661 ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg gattcttcag
    39721 atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga gagagaagga
    39781 ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa ccattttcac
    39841 cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc agtaatgttc
    39901 tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg tggagttcaa
    39961 gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt gtttctccta
    40021 ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt tgtacactgt
    40081 ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc tttttactgg
    40141 acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt tccaatcgtt
    40201 ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc aattcctttt
    40261 gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct tttacttccc
    40321 agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa accagcgtac
    40381 cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct agatgggttg
    40441 ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc aactcgatct
    40501 ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt aactggaaat
    40561 gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat tgaggcaggt
    40621 agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca ccggctatta
    40681 gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata cgatcttgct
    40741 gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg acttgtagat
    40801 ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat gtaaaacaac
    40861 aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc atcacatctt
    40921 ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag ctttcaccta
    40981 ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt ttatacatgt
    41041 cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc gatgcagtca
    41101 tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag caatagctac
    41161 tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg ctaaacgaac
    41221 agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg gaacataacg
    41281 gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac caattggatt
    41341 tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta agctagaatc
    41401 gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag gaatgatcgc
    41461 agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag cttggttgta
    41521 gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca ttgcagaaga
    41581 agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt ttgcttcctc
    41641 gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg cagtcgtcgc
    41701 ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat catatacgcg
    41761 aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca ttattattaa
    41821 cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct aaaagtgtta
    41881 gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct gtatctttat
    41941 ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct acatacactt
    42001 cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc aataccttca
    42061 gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg ctttgtagca
    42121 atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt ataccgatat
    42181 tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat aaaaggtgac
    42241 taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca acgattgact
    42301 tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac
    42361 gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg aaaaaatgat
    42421 aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata gaagatagct
    42481 cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt tgcaagaatc
    42541 ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag ttatgacaag
    42601 atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag
    42661 ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc
    42721 agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct tcatatctcc
    42781 agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga tgggtaccag
    42841 ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa taaaaactta
    42901 gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt gccattgtgt
    42961 aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg ctgtttggtg
    43021 cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt ttttctccgg
    43081 cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac atcggagaag
    43141 ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca ttgttcaatg
    43201 tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta gcatctttgt
    43261 aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc tgaaaagaag
    43321 tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga tattttctaa
    43381 aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc atcgtaacag
    43441 attggccatt atagcttcct ttaatcccaa atagattgta attaggggca gaagccaaag
    43501 tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat gcatataagt
    43561 cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac gcttgttgag
    43621 aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat tcagacaaat
    43681 ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg atttcttctg
    43741 cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt gtttgttctg
    43801 ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta gctgactgca
    43861 aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc tgagttgcga
    43921 cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga tttcctaaaa
    43981 gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga attatgaaac
    44041 ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca tatttaaata
    44101 acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa atgaagaata
    44161 ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa caagtaattc
    44221 tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga taatcatgtt
    44281 gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag ctatatcttc
    44341 agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct ttttgtcgct
    44401 gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag atttgatgtt
    44461 attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa attttgttcc
    44521 gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag ttgaaatgat
    44581 atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat ttaacacacc
    44641 catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa ctaatggagc
    44701 ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact tttccatcgc
    44761 taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt gaatatatag
    44821 ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc gtgcagctat
    44881 aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga ttccgcttga
    44941 agcgcccatg atgacaatca ctttttctga taaatcattc attctattct cctcctgttt
    45001 tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc aaataatccg
    45061 ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt ctgggacatc
    45121 tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag
    45181 aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct ttactgacaa
    45241 gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga cacgaggacc
    45301 aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca cttgacgatt
    45361 gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa aaggcagcag
    45421 aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt tgcggatatc
    45481 tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat
    45541 ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga cgatttgttt
    45601 ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac ggacggctta
    45661 tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact tagtggtatg
    45721 cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt ttttagcagt
    45781 agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc aacctactct
    45841 ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca ctcgaggctc
    45901 ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa aagaaatctt
    45961 gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct ctggagaaat
    46021 ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt
    46081 gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag atgatatcat
    46141 agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga caaacaaaca
    46201 aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga tacatcggaa
    46261 tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat
    46321 catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt tcagcataga
    46381 tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga tggtcgataa
    46441 cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga cggaaaaagt
    46501 attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga aaaaagacca
    46561 gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat acagttaagt
    46621 agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca aaagatccaa
    46681 tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat aattccagta
    46741 aattactata attataaaaa aagaattgac aaaacataac tgtctatata aaattgatta
    46801 cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa tctctgagga
    46861 acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat caactggtct
    46921 agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa aaagatcttt
    46981 aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat tggaaaaggg
    47041 attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga acggatattt
    47101 gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat tgatgaacgc
    47161 tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat ggaaaagttg
    47221 aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga gaacattaat
    47281 aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta cagtgatgaa
    47341 ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat tccatatact
    47401 tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg tcggaacaat
    47461 aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt agtggaatca
    47521 gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag actggtgaat
    47581 catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt agacacggaa
    47641 atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa aaaaatccgt
    47701 gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg ttacttggcc
    47761 atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa tcatgaggag
    47821 gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt ccagttgcgg
    47881 tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct tatagtaacg
    47941 acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa ttacctttgc
    48001 tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct gctgccttgg
    48061 caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca gtagctactt
    48121 tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt aacgtattta
    48181 ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac gaagcaaaat
    48241 taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg gctgcctttg
    48301 tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac tctgctttag
    48361 tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt tatggcttct
    48421 tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg ttctggtttg
    48481 acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca aaaggcgtaa
    48541 caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca gcaggagcat
    48601 atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc ttgatgttag
    48661 cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc tcatttatgt
    48721 tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct ttagcagtga
    48781 gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc gattacttcc
    48841 tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa ggattagtaa
    48901 cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat ctgatgactc
    48961 ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat tcagacaata
    49021 aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac aatgtaactt
    49081 cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc ctgatcgatc
    49141 aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga aaaaatgcgc
    49201 aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa ctacatggtg
    49261 gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca tttgccggtg
    49321 gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca atcactgaag
    49381 tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta ttaccagaaa
    49441 atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca acgaaacatg
    49501 ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc atcaacactg
    49561 ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa gtcgcaagag
    49621 gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa aacacggata
    49681 tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa cccgcacgtg
    49741 atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc atcgtcttcc
    49801 ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc tcagctcctt
    49861 tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca gccaaacgta
    49921 acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga acagcttttt
    49981 tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc aaatttatgc
    50041 aaaaatgtgg aaagccattt tcacaaaat
//
LOCUS       DAZAOG010000006        49822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000006 DAZAOG010000000
VERSION     DAZAOG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.006"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..1462
                     /locus_tag="ACHDLD_000282"
     CDS             242..1462
                     /locus_tag="ACHDLD_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN3362046.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1459..4179
                     /locus_tag="ACHDLD_000283"
     CDS             1459..4179
                     /locus_tag="ACHDLD_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3362047.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4163..5107
                     /locus_tag="ACHDLD_000284"
     CDS             4163..5107
                     /locus_tag="ACHDLD_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3362048.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5176..6186)
                     /locus_tag="ACHDLD_000285"
     CDS             complement(5176..6186)
                     /locus_tag="ACHDLD_000285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3362049.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6366..6710)
                     /locus_tag="ACHDLD_000286"
     CDS             complement(6366..6710)
                     /locus_tag="ACHDLD_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN3362050.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6703..7137)
                     /locus_tag="ACHDLD_000287"
     CDS             complement(6703..7137)
                     /locus_tag="ACHDLD_000287"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN3362051.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7275..8012
                     /locus_tag="ACHDLD_000288"
     CDS             7275..8012
                     /locus_tag="ACHDLD_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362052.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8005..9216
                     /locus_tag="ACHDLD_000289"
     CDS             8005..9216
                     /locus_tag="ACHDLD_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362053.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9420..10202
                     /locus_tag="ACHDLD_000290"
     CDS             9420..10202
                     /locus_tag="ACHDLD_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN3362054.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10272..10934
                     /gene="trmB"
                     /locus_tag="ACHDLD_000291"
     CDS             10272..10934
                     /gene="trmB"
                     /locus_tag="ACHDLD_000291"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN3362055.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKSSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11032..12786)
                     /gene="ade"
                     /locus_tag="ACHDLD_000292"
     CDS             complement(11032..12786)
                     /gene="ade"
                     /locus_tag="ACHDLD_000292"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN3362056.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12918..13847
                     /gene="comGA"
                     /locus_tag="ACHDLD_000293"
     CDS             12918..13847
                     /gene="comGA"
                     /locus_tag="ACHDLD_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN3362057.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13804..14853
                     /gene="comGB"
                     /locus_tag="ACHDLD_000294"
     CDS             13804..14853
                     /gene="comGB"
                     /locus_tag="ACHDLD_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN3362058.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14850..15128
                     /gene="comGC"
                     /locus_tag="ACHDLD_000295"
     CDS             14850..15128
                     /gene="comGC"
                     /locus_tag="ACHDLD_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN3362059.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15103..15573
                     /gene="comGD"
                     /locus_tag="ACHDLD_000296"
     CDS             15103..15573
                     /gene="comGD"
                     /locus_tag="ACHDLD_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN3362060.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15545..15892
                     /locus_tag="ACHDLD_000297"
     CDS             15545..15892
                     /locus_tag="ACHDLD_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362061.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15855..16280
                     /gene="comGF"
                     /locus_tag="ACHDLD_000298"
     CDS             15855..16280
                     /gene="comGF"
                     /locus_tag="ACHDLD_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN3362062.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16252..16611
                     /gene="comGG"
                     /locus_tag="ACHDLD_000299"
     CDS             16252..16611
                     /gene="comGG"
                     /locus_tag="ACHDLD_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN3362063.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16783..17790
                     /locus_tag="ACHDLD_000300"
     CDS             16783..17790
                     /locus_tag="ACHDLD_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3362064.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17815..18999
                     /locus_tag="ACHDLD_000301"
     CDS             17815..18999
                     /locus_tag="ACHDLD_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN3362065.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19436..21019)
                     /gene="abc-f"
                     /locus_tag="ACHDLD_000302"
     CDS             complement(19436..21019)
                     /gene="abc-f"
                     /locus_tag="ACHDLD_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN3362066.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21124..21252)
                     /locus_tag="ACHDLD_000303"
     CDS             complement(21124..21252)
                     /locus_tag="ACHDLD_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362067.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21597..21740)
                     /locus_tag="ACHDLD_000304"
     CDS             complement(21597..21740)
                     /locus_tag="ACHDLD_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN3362068.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21962..22441)
                     /locus_tag="ACHDLD_000305"
     CDS             complement(21962..22441)
                     /locus_tag="ACHDLD_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3362069.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22514..22798)
                     /locus_tag="ACHDLD_000306"
     CDS             complement(22514..22798)
                     /locus_tag="ACHDLD_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362070.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22876..23070)
                     /gene="ssrS"
                     /locus_tag="ACHDLD_000307"
     ncRNA           complement(22876..23070)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHDLD_000307"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23142..24428)
                     /locus_tag="ACHDLD_000308"
     CDS             complement(23142..24428)
                     /locus_tag="ACHDLD_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN3362071.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24566..25237
                     /locus_tag="ACHDLD_000309"
     CDS             24566..25237
                     /locus_tag="ACHDLD_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN3362072.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25203..25691
                     /locus_tag="ACHDLD_000310"
     CDS             25203..25691
                     /locus_tag="ACHDLD_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN3362073.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25704..26651
                     /gene="prmA"
                     /locus_tag="ACHDLD_000311"
     CDS             25704..26651
                     /gene="prmA"
                     /locus_tag="ACHDLD_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN3362074.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26652..27416
                     /locus_tag="ACHDLD_000312"
     CDS             26652..27416
                     /locus_tag="ACHDLD_000312"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN3362075.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27649..29862
                     /locus_tag="ACHDLD_000313"
     CDS             27649..29862
                     /locus_tag="ACHDLD_000313"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN3362076.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29873..30319
                     /gene="dtd"
                     /locus_tag="ACHDLD_000314"
     CDS             29873..30319
                     /gene="dtd"
                     /locus_tag="ACHDLD_000314"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN3362077.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30490..31749
                     /locus_tag="ACHDLD_000315"
     CDS             30490..31749
                     /locus_tag="ACHDLD_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN3362078.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31838..32785)
                     /locus_tag="ACHDLD_000316"
     CDS             complement(31838..32785)
                     /locus_tag="ACHDLD_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3362079.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33179..34471
                     /gene="hisS"
                     /locus_tag="ACHDLD_000317"
     CDS             33179..34471
                     /gene="hisS"
                     /locus_tag="ACHDLD_000317"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN3362080.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34486..36255
                     /gene="aspS"
                     /locus_tag="ACHDLD_000318"
     CDS             34486..36255
                     /gene="aspS"
                     /locus_tag="ACHDLD_000318"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN3362081.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36364..37239
                     /locus_tag="ACHDLD_000319"
     CDS             36364..37239
                     /locus_tag="ACHDLD_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3362082.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37505..39388
                     /locus_tag="ACHDLD_000320"
     CDS             37505..39388
                     /locus_tag="ACHDLD_000320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3362083.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39463..39747)
                     /locus_tag="ACHDLD_000321"
     CDS             complement(39463..39747)
                     /locus_tag="ACHDLD_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3362084.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39737..40471)
                     /locus_tag="ACHDLD_000322"
     CDS             complement(39737..40471)
                     /locus_tag="ACHDLD_000322"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN3362085.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40600..41223
                     /locus_tag="ACHDLD_000323"
     CDS             40600..41223
                     /locus_tag="ACHDLD_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN3362086.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41415..42536
                     /locus_tag="ACHDLD_000324"
     CDS             41415..42536
                     /locus_tag="ACHDLD_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN3362087.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42543..45671
                     /locus_tag="ACHDLD_000325"
     CDS             42543..45671
                     /locus_tag="ACHDLD_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFN3362088.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45690..48884
                     /locus_tag="ACHDLD_000326"
     CDS             45690..48884
                     /locus_tag="ACHDLD_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN3362089.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49030..49737)
                     /locus_tag="ACHDLD_000327"
     CDS             complement(49030..49737)
                     /locus_tag="ACHDLD_000327"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3362090.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16247 a         8781 c        10411 g        14383 t
ORIGIN      
        1 gaaaaatgga gcataaattc cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat
       61 ataacataga tatttaggat tgtgacaaaa atcatgtcac aatctttttt tcgaataaac
      121 ttattttttg aagtcggcca tttatcgtaa tttctttgca taatacgaac aaattttcgg
      181 ttattgatga aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga ggaaattttt
      241 aatggtgaaa tttatccatg cggcagattt acatatggat cggtcatttg aaggactgac
      301 aactcttgat aaaactgtac aggaaaaact attgaagacg aatctgaccg ttttatcaaa
      361 tatcattgaa caggcaataa taaataacgt tgattttgtt ttacttgcag gagataattt
      421 tcatcaaaat cgtccttcat taaaaataca gaaacatttt tcagaacaga tgaaacggtt
      481 agaaaagaac catatacctg tatttataat attcggaaat catgatttct atcaaaaaga
      541 gcggtattgg ttctcctttc ctaaaaatgt ccatttattt acatccgaaa cggtagagac
      601 aaaaaaaata acgataaaaa gtggagaaac tgtctctctc agtggattta gttatcgtca
      661 accgtggatt caaaaagaca aagtgatgga atttccttct agagaactca cagattacca
      721 tattgggttg taccacgggg aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca
      781 accctcaaag atgcaagaaa agggatacga ttattgggct ttagggcata tccatgtgcc
      841 aacggtctta aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag gtcatactaa
      901 aaaggaacaa gcttctacat ctattctact agttgaatta agtaaaggaa gttgccaaat
      961 acatccgatt aaagtagcag aggtctattg gaagacaaag gaaatatcat tgaggaaagc
     1021 aagaacgaca aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg
     1081 atttagttta attgagataa aattaaaaga ttatgaccat ctcaatacag atgttttgga
     1141 acggatacaa tcaggagagc tattagacta tttgttagag gaattctcag ataggataaa
     1201 tgatttcttt atttggcgta tttctctcat agagaatact ttttatacga agaccccact
     1261 tgctgcttct gctaaattga tggaacagtt atttcaatac tatcagacac ctcaagattt
     1321 ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg gctagaatat tgagcgaact
     1381 gccggaatat caagaagaaa cacttgaaaa agccaagcag ctgcttaatc aagatttttt
     1441 atttgaggag gatcaggaat gaagatttta aggatcgaga ttgctgcttt tggaaaatgg
     1501 cgtcaaaaaa gttttgactt ttattcaggc aatcagctga tctatggggg aaacgaagca
     1561 ggaaaatcaa cgatttatca atttattcaa gcaatattat ttggtttccc ttcaaaaggc
     1621 cgtaaaaaaa aggattatac accaaaagac ggctcagcct atggcggcaa aatatggctt
     1681 aaacatcctg tatatggtga atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa
     1741 tcgaaggtgt ggctggggga tcaagtggga agtgatgagt tactagaaaa actgatcgca
     1801 cctttgacga aagagttatt ccagcaggta tttactttcc agcaagagca gttgatggaa
     1861 ctagatcatt tgagagaaaa agagctacat gatgcgctga tttctttagg catcacgggc
     1921 agttctcaag tattccaaaa gcggcaagaa tattatcaga aagcacaaca attattcaag
     1981 aaaaaaggcc aaaaattact aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa
     2041 aaaattcatc aaaaacaaga acaggaagct tattttggca agttgatcca acaagaacag
     2101 gaatacgagt cgcaacagca gcagttattg gcacaattaa aagaggcgaa caaacaattt
     2161 tcattagcca gacagcaaga acttaatttt tctttatatg aagaatggca atctctcaaa
     2221 aaaaacagca ctacaggtct tcctgatgag caacttcttt tagatctgga gtctttctcc
     2281 aaacggtatc agcaaacaaa tgagcgtttg gaagaattag aagcaacact ggaaaagcat
     2341 agcgggatgg atcagcagtc agccagatat tatttttatt tagagcagga gcaggaacta
     2401 aaagatctcc agcaacttca atttacagcg gaacaattaa acgaagaatg gcaaagatta
     2461 caaattgaga ctagagaaac aaattcagaa ttgaagattt tggaacaaag gtggcattgg
     2521 cagaaaaatc cgttaccgga agaattttta tatgaagaag aatggaagca tcttctggct
     2581 gaagaaagaa ggattgctga tctagaaagc cagatttctg tgcgtctcca aatagctaaa
     2641 gaacagcaag agatgattga agcagaagtg aatgacttgg aagagaccta tccagcatta
     2701 ttacaaaatc agtcagagat agcaccagtc tcttcatcta aaccgtatat gctgatggct
     2761 ggcggagcaa taatcattgc aggtatattt gtaccaatgc ccttgaaagt gatcctttta
     2821 ctaattggtg caggcatgat tggctatttc ttctacaaaa ggcaaccaaa aattaaaact
     2881 aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa aactaggaca acttgatcta
     2941 gccttagctc aattagctga agcagaagaa aaacaaaaga gaactggaga agaaaaacag
     3001 attcttcagc aagaagtata tcgttttgcg gatcatcggc acttaggaaa gatggatacg
     3061 ctcaaactca tgaaagaata tggacaggaa gtcatagatt atcagaaatt atatggcgta
     3121 tttgagcgga aacataaaag agaacaggaa atacaagaaa aactacgaca aatcgaccaa
     3181 aaatttgagt ttgttaaaga atggttaccg atacaagata agacaatttc agaaaaaatg
     3241 gtattgcttt cccgttttat acaagagatg gaacagatga aatttgccag aagctatcag
     3301 caaaatactt tattgaagca agaaatcaat caattgaaaa agaaacaaaa agtatggata
     3361 caagaataca gcgaacaatt gactcaagca ggcttatctt atccttcgga agtacctgtc
     3421 tttttacaaa aagccaaaca agccgaacag cagatgaaaa gatacaatga attatcggaa
     3481 atgcttactc cactatttcc tgaagagctg acaaaagaga aattatctgt aagactgcgc
     3541 caactgaaag aaaagcaatc ccaactccag caagaagaaa aagaagcaag tgaaaatcgg
     3601 caacgactcc aacttcaagt agaagagttg caaagagacg gcacattgga cgaactttat
     3661 caacaagcta gccggcaaaa agcaaaatta caggaattac tggaggaatg gggagcactt
     3721 gaaatcgccg ggacattctt aggtgatctt tcaacagaga tgagtgaaag acaattaccc
     3781 cagttgttaa gagcagcagg tgattattta ggtattttaa caaaaggaaa ttatcagaag
     3841 atattactga aaaatgatac actgacttta acagacggat atacagaatt tcccatctat
     3901 gagttatcta caggtacgaa agatcagctg attatggcga tgagatttgc ttacctctca
     3961 ttagaagcta accgttcgat atgcccgatc atcattgatg atggctggct tcattatgat
     4021 catcagcgaa agtatcaatt agctgaactt ttagctaaat ttggagaaaa gtatcaaata
     4081 atttgttttt cttctgataa agaaatggta agctattacc atgagatgaa ccaaatcgta
     4141 aaacaattag aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt
     4201 tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt
     4261 tattgctttt actttccaag atacttcagg tactatcgat ggtaaatact gggatgcttc
     4321 ggaggaggaa atcaataaat ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga
     4381 ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa gggtagatga
     4441 gccaggagag cctagtttat acatggaaag agcccctttg aagaaagaag aaatggaaga
     4501 agaaatcaat caattgttgt ttgagatcac caatgcgcat tggaatcgta ttgttcgcta
     4561 tctacttaat aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa gaaaccatca
     4621 tgcatttgcc agtggattag cttatcatac tttgacaatg cttcacttag ggaaatcaat
     4681 cgccgatgaa tatcctgagc tgaatcgagc attgttgtat gctggtatca ttttgcatga
     4741 tttaggaaag gtgattgaac tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa
     4801 tttgatcggt catctggtat tgattgacga agaaataact aaagcctgtc ttgcgttgaa
     4861 aatcagcgaa gctgacgaag atgttcttgt attacgtcat atggttttag cacaccatgg
     4921 cttattagaa tatggctcac ctgtacgtcc aagaataatg gaagcagaaa tattacatca
     4981 aatcgacaat atcgatgctt cgatgcagat gatgttgact tcgattcgtc aaacagaacc
     5041 aggaaaatac accgatcgga ttttcggtat ggacaaccga agtttctatg taccaaaaga
     5101 tttatagcaa aaatttattt ttttcattca aaacaaaaaa agcgagacat aagtcttcgc
     5161 tttttttgtt ttcttttatt ttgatgaatc agttgtttct tcagttgaag attcttttgt
     5221 tgaatcagtt gattttgtgt cagaactctt tgttgaagct gtagtttcac ttgagtcttt
     5281 cgtacttgat gaagttgttg tgaatgcact caacacattt tcaaaggcat catctttgat
     5341 ttttacattt gcatctttta attcttcacc gattacttta gttgtgaatg cattgtcact
     5401 tagtttagtt tcagtagcga tatcttttag ttgatctttg tatttatcca tgtcattgcc
     5461 tttattttgg tttttcacca ttttgacaac gtagtattct gtcgcataag aagtaggatt
     5521 tgttgtagta ataacatcgg agatctcacc gtctttcaat ttgaaagctg cttcttttac
     5581 ttctgcagga atagttgttg tcgttgagtc gaatttaact ttaccgccat cttcttttgt
     5641 ctctgtatca gttgatttgt ctttggctaa tttagagaaa tcatctccat catccgctga
     5701 ttttttcaca tcttttgctt catcttcact tgataatttg atgatttgtg cttctacttc
     5761 tggatggaaa gatttccaag cagttttcaa atcgtcatct gtaatatcta cgtgagcttt
     5821 caagccagct tcaaaagcta aattgttacg aatatattct ttataagtat ctttcgtata
     5881 tccagcagct tctaattgtg attcaaaagt atcacctaat gatttcgctt gtttgtcgta
     5941 ttctgcatcc acttgcttat cggttacttt atcgccatat ttgttattga atactttata
     6001 aatgatcatc cgttgtacta aggattggtt cgatgattca agttttgctt catcatagaa
     6061 atcagataca gtgatcgttc cgcctttcat cgttgcgata tctttattag tatcgcctga
     6121 acaagctgct agagttaaaa cagccaacgc actagtagct gccaaaataa ttgatttttt
     6181 tttcattaaa atttgcactc ctgtaattag ttattatttt tatatattaa ctataccata
     6241 tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac
     6301 attcctagga aatacgcaaa cttttctcac ttatatagtt aaatctgttg agaaaataat
     6361 cattattata tttgttcagg atactctggc agatcagctt ttagtttttc tacttgttca
     6421 ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc ttttttgatt
     6481 gcttcttgga aagcaggaat cacagattca gctgtttctt ttgtttcgat caatgcgctt
     6541 ttaaaatgat tcaggctgtt atttaaatca tttgtcagag ttgttgcttc ttccaattct
     6601 tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg ccaataacag acctccgaca
     6661 gtgccagcag cagctccaaa taacagtcct ttgaaaaagt ttttaatcat cgcaggacac
     6721 ctgctttacg attgtttctt gaatagcttt tagttcttcc gatccataac tatcttgatg
     6781 attgacaaag tgaatagaaa aatcatcctc tttgctgtag cgagggacta aatggatatg
     6841 tgaatgaaaa acagattgat aggctagttc acggttgtta ttcaagatat taagaccttt
     6901 catttctgga aaagcttttt ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc
     6961 tcctgcaagt tcgggatcat attcaaaaat atctgctaca tgacgtttag gcacaactaa
     7021 tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca tagaccttgt cgtcttcata
     7081 tattttatag cttgggattt cttgattgat gattttgcaa aaaatacagt tttccatata
     7141 aaacatcctt tctataaaga ataaatactt cagcttaact ttaccataag ttaaagctat
     7201 tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt ataataaata aaaataaatg
     7261 gcggaggaaa aagaatgagt ttaattatcg atcatgtcac aggggggtac ggacatctcc
     7321 ctgtgttaaa agatatcagt ttcgatgtac agcctggaga gatagtaggg ttgattggac
     7381 tgaacggagc agggaaaagt acgaccatca agcacattat cggtctgctg gaagcttcaa
     7441 aaggaacgat agaaatcaat catctatcct taaagaaaaa tacggaagaa tatcgcaaac
     7501 aaattgggta tattccagag actccttctt tatatgaaga attgacattg agagaacata
     7561 tcgaagtcac tgcgatggcc tacgatatac ctatggatat agcaatgaaa agagcagaaa
     7621 agttgttgga gacttttcgg ttatctaatc gtcttgactg gttcccagct aatttttcaa
     7681 agggaatgaa acagaaagtg atgatcttat gcgccttttt gatcgagcct tctttatata
     7741 tcatcgacga accattttta ggcttggatc cactagctat caatgcattg cttgaattga
     7801 tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt ttagctactg
     7861 cagaaaaata ttgtgatcgt tttgtggtct tacataacgg agagatacga gcagacggtt
     7921 ctcttgagat gttgagaaaa gaatttcagc tgccggattc ttctttggac gaaatctata
     7981 ttgctttaac aaaggagcag gcacatgagt agtttttttc aaaaacgttt agcaagacat
     8041 caaaagaaaa tgatgaaata tatgcgttat gtattgaacg atcattttgt tttagtttgt
     8101 cttttcttga ttggcggtgt gggattatat tattctaatt ggttgaaaac cctgcagcca
     8161 ccgtttgcaa ttggagggct cataatctct gtattttgga tgctctgttt attcgtgggt
     8221 aagatggcta cttttgcaga ggcagcagat attgtttttt tacttcctaa agaaaaagaa
     8281 atgcaaacct atttggaacg agcttttcgc tattcttgca tttttccgtt gatctttttg
     8341 atattggcat gcggattggc catgccatta gtggtggtta gtacggagca atccttttcg
     8401 atgtttttcg tctatctgat catattatgg agtttgaaat tcagtcatct gcaaatcaag
     8461 agagcagaat tgtttcgact agatcaacaa gttattcgtt attggaaatt aggctggttt
     8521 ttttcttctc tgatcttttt gtttctatca ttttattggc taatatgggc aggaatagca
     8581 ggtgcattag tacaggcaag tctatattac tggtttttgt ggaagcaaca agaaaaagga
     8641 ttggattggg agaaaatggt gcatgtagaa gaacataggt tgcatcagat ctatcaattt
     8701 atcaatttat tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag acgctatttg
     8761 gatcctatat tgactcatat taaaagagaa tcaaaaaaca cttatcttta cttgtttgca
     8821 agaagattgg caagaggttc tgattttagt ggtctttatt tccgattagt aattataggt
     8881 gggttattga ttgccggagt agtcgacttt tattttagtc tgattatcgg tttcttattt
     8941 ttatatttga tcggtttcca actgttacct ttagcgaacc agttccgcta tatggttctc
     9001 gttcagcttt atccaatcca taagaatcag aagaagcagg cgattcagta tctactcaca
     9061 tgggtattaa ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt attagatggg
     9121 cagcagcgat gggttccgct agcggcttat ttgattgtta ctatgctctt tgttaaacta
     9181 tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa
     9241 tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt cgttctattt tttttgagaa
     9301 aagctggttt tttgaatggc aattaaatga aaaagaacgt atatgtgctt gacgtgtagt
     9361 gttcaggcga ctagaataat aagtactgca aaacaaagag taagaggacg ggagaatgca
     9421 tgatggagtt tcagttagac caagagtggc ggttacgtcc catcaaaggc gacacaggaa
     9481 aaacatacat aggcatgcgt gatgaggaca aagtctttat taaacgaaac acgactccga
     9541 tgttggctgc tctttcaagg gaaggaatta caccaaaact tgtttggaca aaacgaacgg
     9601 ggaatggcga tactttgaca gcacaagagt ggctggaagg tcgcttgctg actcctaatg
     9661 agattggtca aagaaacgac gtgattgatg ttttgtacca tttacatcat tcaaattctc
     9721 ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac gccggagaag atgctgcaaa
     9781 aatatgaaaa agaattaccg gaagaactcc agaaaaatca atttctgcaa attgtccatc
     9841 gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa cttatcagta gtacacggag
     9901 atgtaaacca tcgtaattgg ttagtatgta aaaattattt atatctagtc gattgggatt
     9961 caatcatgtt tgcagaccct gcggtggata ttgcaacgat tttaggaaat tatgtgccac
    10021 tgtctagctg gaatcagtgg ctgatcagtt atggcatacg cccgactgac gaaatgcttg
    10081 aaaaatttca ttggtatgcg gccatgaatt tgctccaaga aatcaccaga tattgcaaac
    10141 gtgaagatca tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct
    10201 gaggtttggc ccaaactctg cggagatttg gccagacctt tttaattaga ggaaaggaat
    10261 ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc
    10321 cgaattttgt aattagtgat ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa
    10381 acgatcatcc aatccacatt gagattggta tggggaaagg ccagttcatc actggaatgg
    10441 caaaagccca tcctgaaatc aattatattg gagtagaaat gcaggtcagt gttgtttcga
    10501 ttgcactaga caagttgatc gaacaaccgc ttcctaacct gaaacttcta catgtggatg
    10561 gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt
    10621 ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag acttttcttg
    10681 ctgtagatga acagattctt cgtccaaatg gagaaatcca tttcaaaaca gataatcaag
    10741 gattgtttga gtattctctg gcaagttttt ctcaatatgg catgatcctc aaacaggtat
    10801 ggttagattt acatcaaagt caatttgaag gcaatattat gacggagtat gaagaaaaat
    10861 cttcttcaaa aggtcaacgt atttatcgag tagaagctca atttcaagat aagaccaaaa
    10921 agaaaagtct gtgaaactaa ctttctgctt tcataaaaac attaatatcc ataaataaat
    10981 ctcagaaaat gaacattaca gatgtaatgt attttctggg atttatttga tttaaaatgt
    11041 atttatttca tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa
    11101 tcctttgtcg gtgatcttca attcgggtat gacagataat gttaagaaag aaagtgtcaa
    11161 aaaaggatca aaggttttag ctgctcctag ttgatgtgct ttagttatta attcattcaa
    11221 ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag gaagagaaac
    11281 acaagctatt tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag
    11341 attagcagca tacagcatat cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc
    11401 atgggcaatc gttgtcgcta tcgctccttg tttcagctga aatcctttca ctatgcctaa
    11461 tccaatgttt cccgtttgat ggtgacgctc gatcaccgca atctttaatt ggtctttttc
    11521 gattgaagga atgaatcgtt cgccttgtcg gtccactttt tctaccaaat ctttagtcac
    11581 tagactattc gggataattt cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa
    11641 cgaatgtgga tttaatggtt gagcgtttag ttttggcaga caacacgtat atgaatttga
    11701 atgatcagca aataaatggt cttggagttg cccagattga acaactaatg ttcccttata
    11761 gaaaacttca tgaattggca gcttggtaag atcgtctgtt aagaagaaat cagcttgata
    11821 cccaggggca atcgcaccta gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc
    11881 catctgaatg gcttgtaatg gatcgattcc aagtgcaata gcttgacgga cgatatgatc
    11941 gattgatcct tcttttacta aatcattgat cagtttatca tcggtgacaa agaagcatct
    12001 tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc agatcttttg caacggtccc
    12061 ttctcgaacc attaaataca taccaagatc caaacgttct ttagcttctt ttgcagttgt
    12121 agcttcatga tccgtccgaa tacctgctgc aggataaaca tttaactctt ccatgccgat
    12181 accagctgca tgcccgtcaa tttttttctt atgctggtgc atcaattgga tcttttctag
    12241 gatgtctgtt tcattatttg ctactgcttg atagttcatg acttcagcta agcctaaaac
    12301 ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt aaatttgcac cattggtctc
    12361 aaaaggggtt acaggtacac aagaaggtag cataaaaaag atattcatga gtgtttgtct
    12421 tgcatcttct atcataaact caagacctgt actcccagca atgtttccta tttcatgggg
    12481 atctgtcact acagtagtaa ctccatgaag caaggatact tttgaaaatt cgcttggtga
    12541 taaaagtgag ctttcgatat gcacatggcc atcgatgaat cctggcacta gatattgccc
    12601 attagcatta tgtacgcttt ttccttcgta ttcaccgatt cctacgatca ttccttcgca
    12661 gattgctaca tctttttcca tgatttcttt cgtaaagacg ttgacgatct tagcatgctt
    12721 gatcactagg tcagcaggct tttttttact tgctacttcg atatatttcc ttaatgttgt
    12781 ttccaatgga gtgcctccca agataaattt ttataagtat aacataaatt cggcttttgc
    12841 caatattttt ttgtaaattc attttatttg aaacaaaaag atcaaaaaga cgtatgtttt
    12901 ttgtggaggg gagaataatg gaaataaaac gattatcgga tcaaatgata agtgaaagtt
    12961 ttgccaaaca tgcccaagac gtatatatat tgccagcttc agaagtattt tacatctatt
    13021 ttcgctatgg aaaaaaacga acattctgga agaaagtctc ttcggcagat ggacagcaac
    13081 tgatctcaag atttaagtat ttgggacaga tggatatcgg agaaaaaaga aaagctcagt
    13141 taggtgcatt tacttattct ttagaaaaac aggaagttcg tctgcggttg tcttctgtag
    13201 gagattacct tcaacgagag agtttagtga ttcggctatt atacggtact tttgaaacac
    13261 cccatcgttg ctttagttct cgagattttg caactatttt acagaaaacc gtcagacgtg
    13321 gattatacct tttcagcggg acagtcggat caggcaagac ttctctaatg tatcggttgg
    13381 ctttggaaga aaaattgcaa gtgattacta tcgaagatcc tgtagagata gaagaaccgt
    13441 tttttctgca gctacagatc aatccgaaaa tagcacagac ttatgataca cttttgaaac
    13501 tcgcattacg gcatcgtcca gacttgctaa tcattggcga gataagagat gaatcaactg
    13561 cacatgctgc ggtaagagca gcactaacag ggcaccgggt ttttgcaacc gtacatgcac
    13621 gcaatcttga aggtacattg gtcagaatcc atgaactgac aaagaaaaaa gaagagctat
    13681 ctgaatgttt agctggtgtc atttatcaag aacttttgga agatgccaat caccatcctt
    13741 ctgttttatg gagttatcaa tttatggagg aagaatcagt aaaaaaatca tgggaggaaa
    13801 gttatgaaga agctcaggaa aatatttggc agtcagaact attctaaagg attaacaaga
    13861 agccagcaaa accagtttgc acaaaccatg ggagaactgc ttttatctgg ttttagtctg
    13921 caagaatccc tttatttatt actcacgatc cgttcgtttc cacctgttat tttggcaaat
    13981 atccaacatc agctaaagga agggcaagcg tttagtgagg tactttcgca aataggatat
    14041 ggagcagagc aactggtaca gatcgagtta gctgaacgtc atgggaatct gccgacaact
    14101 ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag
    14161 ctgcttgcat atccgatgct attgctcacc tttttgctag gtatcttgtt tagtatgcgg
    14221 ttgttcgttt tgcctcagct tattggttca ggaatggtca tgcaagagca ttggggtatt
    14281 cagctgatac aagtctttca ttggtatgtt ctactattca tcctagcggt tactctattg
    14341 gctttactag taaggtatcg tctgatgaag ttgtctcctg ttgatcagag tgaatggata
    14401 agcagacaat tattgattgg accgatatat tccttgtatc aatcggctta tttgtcacta
    14461 gagattggaa agctttttca tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag
    14521 gaaacgaaac aagaaagtct gattcagcat cttgcctcta agttgattca aggattggag
    14581 gcaggtatgt cattggctga gcagttccag aattatcgtt ttctatcaga agaattcagt
    14641 aaaattgttt tacagggaga ggcaaaggga agtttaggta aagaactgat tttttacagc
    14701 gaatggacaa gaaaacagct tttcaaacgt cttcatcact ggatgcattt tattcagcca
    14761 atcatttttt tgggcgtagc ggctttgatt ttattagttt atgccgctgt tttattacct
    14821 gtttacggaa gcatagagga ggttttgcca tgaaaaagag aaaaatatct tattatagcg
    14881 gttttacatt gatcgaaatg ctaatcgtct tactgatcat cagtgtatta gtgttgttgt
    14941 tcgtccctaa tttgtcgcgt tataggaatc atgtagacca agaaagtcgt gaagcgatca
    15001 tccagttggt agacacacaa aaagagctct atgcgcttca aaataatgga agagttccta
    15061 ctgtggagga attgttgaat gaaggctata tcaaacgaga acatgcagaa atctatcagc
    15121 ggccttgaag gtaaggtttt agcatcagga tatacgttgt ttgaatcact cttactgatt
    15181 ttattactta caacgatcct ttcttttcca gttctcgcat tttctgcatg gaagaatgaa
    15241 ttggcagtct atcaattttt cggccaattt gaaaaacgaa tctattctac gcaaaaaatc
    15301 gctattgtga atcagatcca aacaggcttt tattggaata gtgaagagaa tcaaatcatt
    15361 tttcatgttc cgaatccaga taaaataaat tgggaagtat tagatattcc tgatgagatt
    15421 accttgaaac gccatgcttc tattactttt gctgctagaa ccgggaatga aagttcacta
    15481 aaagcttatc aattttattg ggaagaaaaa gagcggacga ttacgtatca attccagata
    15541 gggagtggtc gctatacaaa aagaattgaa taaaacagga tttgttttgt tggagtccct
    15601 tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg agctttggcc agctgttttt
    15661 gcttcaggca gaaaagaaaa gtggggagga actgctattt attcgaatga tttatgaaga
    15721 tatcaagacg cagcggatgt atcacaaaag accagagcga agggaagaaa tgatcccata
    15781 cgatatccaa attgtcgata ataatggagt aatcgaaaaa gtgtttgtca atatggagaa
    15841 agaagcgatc agtattgaaa aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa
    15901 ttggactgat gatattgagc agtctattgc tcacattcaa tcttttgatt cagcaaacca
    15961 tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga gtggctaata tttttagcac
    16021 aattagaaga agaactcaag agcagtcaca atgtctctgt gaaaaatcag caactgcact
    16081 acacgatcga ggataaacag tatatcatcg aaagatatca ggcaatgata cgtaaaagaa
    16141 gaacttccgg cggacatcaa cctatgttaa caagtatcac tgagcttcaa ttattggaaa
    16201 aagaacacac aatttctttt tatgtacact ttgaaaatgg agaagatgga tatgccatat
    16261 ggacaaaaaa cgatcagtag aaaatgttat aagggctcta ttttattttc tgtcttgctt
    16321 ttatttcttt tatgtacgtt tttattgatg gcagcattag aaagctaccg tttgtcaatg
    16381 gatcttaata tacgaatcaa aaattattat atagcaaaaa taatgaagca gatggcttta
    16441 tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt tttactacac aactggatct
    16501 gtaacatatc agtgggtaca acagtcccta ttcttagagg tggaagtctc accgtttatc
    16561 tttcggttca cagaagaagt aaaaaatgat acagatacta gtacagaatg atggaatgtt
    16621 cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa taaaaaaatg tttgccgttt
    16681 cacaaataaa aaacagtgaa aacgattgaa attttgtcaa ttgttcgata gaattaaaca
    16741 ggtgaaaaaa ttgttataat atatctagaa atgaggtgca ccatgttacc agaaaaaatg
    16801 gaacaagggt ttaatcaaaa cctggaagct attcagcttt tacagaatgc attgggaact
    16861 tcctttttag aagcctatgt cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt
    16921 gtggtggatg gagtgccgac aaaagagaca acgcaacgga tcacagctct ttatgaagag
    16981 ttgaagaaac tttctttcga gccagaagaa tggcgcagac tgtctcaatt acttttattg
    17041 aagggcagcc agacagaaca tttgcaggcg aaccatcaac tcactcctga tagtatcggt
    17101 tttttatttg tttttcttat tgaacaacta tacacaaata aaaaagcacc ggttaagatt
    17161 ctggatattg cggcaggaat gggcaacttg ttattaacag ttcttttaaa cctatcgaat
    17221 gcggggtacc aaacagaagg aattggtgtg gatattgatg atacattgtt ggctgtcgca
    17281 gcttctacaa gtgatttgac acaagcaaat gttcaatact ttcatcaaga cgggttgcag
    17341 gaattattaa tagatcctgt cgattttgca atcagcgatt tgccaatcgg ttattatccg
    17401 aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag aaggccatag ttatgcacat
    17461 catttattgc tagagcaatc aatgaaatat gtgaagccgg atggttttgg tttgtttttg
    17521 atgccgagtg gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa
    17581 gaaggttatc ttcaaggaat gattcaattg ccagatgaat tgttccgtaa taaacaatcg
    17641 caaaaaagta ttttgatctt acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc
    17701 ttgcttgtga aacttgcttc attaaaagaa ccagagaagg tgacggaatt tttcaacgag
    17761 tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg agaaggagag tcatatgtct
    17821 aaaacaattg caatcaatgc tgggagttca agtttgaaat ggcaattata tcaaatgcca
    17881 gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga ttctattttt
    17941 accatcaagt acggcgataa tcaaaaattc gaacaaatca tggatatcga aaaccacgaa
    18001 gtagcagtaa aaatgctgtt agatcagttg attgaactgc aaattttaag ctcttatgat
    18061 gaaatcaccg gagtagggca ccgtgttgtt cacggaggag aaacttatgg ggattcagta
    18121 gtgatcgatg agaaagtaat ggaccgcatt gctgaattag cagaatttgc tcctttgcat
    18181 aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga ttttacctga cattttgagt
    18241 gtggctgtat tcgatacatc tttccactca acaatgccaa agcaaaatta tttatatagc
    18301 ttaccggtgt cttattatga agatttcaaa gtaagaaaat atggcttcca tggcacaagt
    18361 catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa
    18421 atcatcactt gccacttagg aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc
    18481 atggatactt ctatgggatt cacaccatta gctggggtga caatgggtac acgttcaggt
    18541 gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa
    18601 atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat
    18661 atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc
    18721 gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca
    18781 attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa
    18841 ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa
    18901 gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa
    18961 ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata
    19021 aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc
    19081 aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg
    19141 cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat
    19201 cccaatattt tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac
    19261 atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa
    19321 accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc
    19381 ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt
    19441 caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt
    19501 ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt
    19561 cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat
    19621 ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt
    19681 caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg
    19741 ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac
    19801 ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac
    19861 ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg
    19921 attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac
    19981 tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa
    20041 cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg
    20101 gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga
    20161 aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc
    20221 gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt
    20281 tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt
    20341 cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg
    20401 atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat
    20461 tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga
    20521 ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacag tgtggaatcc
    20581 ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa
    20641 agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg
    20701 tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat
    20761 ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga
    20821 tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga
    20881 tttgtaaaaa ggttgatttg ccagttccgt tgatatcgac taaggcaatt ttttcaccag
    20941 cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag
    21001 aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc
    21061 cctggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg
    21121 attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc
    21181 aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt
    21241 catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag
    21301 tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc
    21361 agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga
    21421 gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac
    21481 ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca
    21541 ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa
    21601 ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa
    21661 aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca
    21721 gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt
    21781 aaaccacacc agggcgacta atcgccctgg tgttcacttt ttttatttta cttgaactca
    21841 ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt
    21901 tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt
    21961 gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa
    22021 gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt
    22081 ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga
    22141 tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt acattattca
    22201 agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag
    22261 cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga
    22321 catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag
    22381 ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca
    22441 taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga
    22501 aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg
    22561 tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa
    22621 aagctgttaa aatacccatt acgcctaaaa cagcatatgc agcaccgtaa aattgtagga
    22681 atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata
    22741 aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga
    22801 acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt
    22861 attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg
    22921 cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt
    22981 taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca
    23041 aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa
    23101 gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc
    23161 tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta
    23221 tcttttggct gataatactg agcattcttc agtttatctg gtaaatattg ctgattgacc
    23281 caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca
    23341 cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata
    23401 tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga
    23461 tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct
    23521 aatacagcgt tcactgtacg agccgcagca gcaggattac ctaaacctat atcttcataa
    23581 ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg
    23641 agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata
    23701 acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct
    23761 tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta
    23821 gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga
    23881 agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt
    23941 aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc
    24001 tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg
    24061 atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct
    24121 aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct
    24181 acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta
    24241 gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga
    24301 atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact
    24361 agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc
    24421 tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca
    24481 agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt
    24541 gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg
    24601 gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac
    24661 accagaagga agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga
    24721 cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa
    24781 accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca
    24841 gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga
    24901 tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa
    24961 attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc
    25021 gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat
    25081 cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa
    25141 tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga
    25201 aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag
    25261 actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct
    25321 tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag
    25381 aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg
    25441 atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag
    25501 tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc
    25561 ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcaac
    25621 aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc
    25681 caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg
    25741 aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg
    25801 aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa
    25861 atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt
    25921 tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc
    25981 tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag
    26041 cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct
    26101 gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg
    26161 catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct
    26221 tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag
    26281 caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt
    26341 cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg
    26401 atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata
    26461 tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg
    26521 tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt
    26581 ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag
    26641 aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg
    26701 ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg
    26761 aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg
    26821 aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt
    26881 cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat
    26941 ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat
    27001 gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag
    27061 cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt
    27121 tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc
    27181 aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct
    27241 tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac
    27301 acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt
    27361 atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt
    27421 cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa
    27481 tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg
    27541 aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat
    27601 ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa
    27661 gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat
    27721 gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga
    27781 aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta
    27841 aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa
    27901 atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc
    27961 gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac
    28021 cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat
    28081 agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct
    28141 caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa
    28201 tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc
    28261 cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat
    28321 attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac
    28381 atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac
    28441 ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca
    28501 atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa
    28561 gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa
    28621 attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg
    28681 gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc
    28741 tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa
    28801 ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt
    28861 actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc
    28921 ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg
    28981 aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac
    29041 tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc
    29101 caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat
    29161 ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt
    29221 tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc
    29281 ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa
    29341 aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta
    29401 cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc
    29461 tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata
    29521 tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt
    29581 ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg
    29641 gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg
    29701 ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc
    29761 catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa
    29821 gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc
    29881 agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat
    29941 tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga
    30001 ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa
    30061 tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc
    30121 ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat
    30181 tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg
    30241 acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat
    30301 tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg
    30361 cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct
    30421 tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg
    30481 ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg
    30541 tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc
    30601 aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta
    30661 ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca
    30721 ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat
    30781 atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag
    30841 aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag
    30901 atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa
    30961 gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct
    31021 ccgattgatc acataaaagg tgtcgagaat cgcaaaaaat cagaaaaatg ggcgcaaggc
    31081 ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct
    31141 tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa
    31201 ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat
    31261 gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa
    31321 gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt
    31381 tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta
    31441 gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc
    31501 aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca
    31561 caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat
    31621 agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa
    31681 gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac
    31741 aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc
    31801 ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat
    31861 caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata
    31921 cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc
    31981 aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat
    32041 cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag
    32101 gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg
    32161 aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt
    32221 ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat
    32281 ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt
    32341 ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat
    32401 ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt
    32461 ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat
    32521 gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct
    32581 cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga
    32641 tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa
    32701 aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg
    32761 aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa
    32821 ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact
    32881 ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag
    32941 tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc
    33001 ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta
    33061 agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg
    33121 ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat
    33181 gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca
    33241 gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac
    33301 accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt
    33361 ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga
    33421 aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc
    33481 gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg
    33541 acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac
    33601 tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat
    33661 ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt
    33721 gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca
    33781 tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga
    33841 agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt
    33901 ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact
    33961 agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca
    34021 agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc
    34081 aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga
    34141 ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga
    34201 aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga
    34261 ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc
    34321 caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc
    34381 aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga
    34441 agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg
    34501 atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg
    34561 gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga
    34621 atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa
    34681 tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca
    34741 gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg
    34801 aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata
    34861 cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta
    34921 agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc
    34981 gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca
    35041 cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg
    35101 ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat
    35161 ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca
    35221 gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc
    35281 ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac
    35341 ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc
    35401 aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca
    35461 ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa
    35521 tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg
    35581 aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat
    35641 gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca
    35701 ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc
    35761 aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat
    35821 gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa
    35881 gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaattaggc
    35941 ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc
    36001 tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga
    36061 ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga
    36121 gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg
    36181 agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg
    36241 aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac
    36301 aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt
    36361 tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta
    36421 tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat
    36481 atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg
    36541 ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta
    36601 ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg
    36661 acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt
    36721 gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta
    36781 tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt
    36841 ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc
    36901 tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc
    36961 gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta
    37021 gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt
    37081 gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct
    37141 ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac
    37201 gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca
    37261 tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat
    37321 cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt
    37381 atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt
    37441 gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata
    37501 ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct
    37561 ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg
    37621 cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct
    37681 gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat
    37741 gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt
    37801 gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt
    37861 caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc
    37921 accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat
    37981 cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt
    38041 aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc
    38101 aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct
    38161 tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat
    38221 tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga
    38281 tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt
    38341 gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt
    38401 atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat
    38461 tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga
    38521 aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc
    38581 acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat
    38641 cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg
    38701 tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat
    38761 cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc
    38821 ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca
    38881 aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca
    38941 agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt
    39001 accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgattggact
    39061 aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga
    39121 tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa
    39181 tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag
    39241 ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg
    39301 gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa
    39361 cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg
    39421 tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag
    39481 caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa
    39541 tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt
    39601 aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc
    39661 taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa
    39721 tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc
    39781 catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt
    39841 tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga
    39901 tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag
    39961 gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca
    40021 cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg
    40081 ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat
    40141 tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat
    40201 ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga
    40261 tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg
    40321 cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag
    40381 catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata
    40441 attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc
    40501 gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt
    40561 tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc
    40621 ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa
    40681 gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca
    40741 ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg
    40801 ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga
    40861 gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg
    40921 agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca
    40981 ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca
    41041 cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta
    41101 tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca
    41161 gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa
    41221 taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac
    41281 tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt
    41341 aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt
    41401 tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac
    41461 aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga
    41521 aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag
    41581 ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga
    41641 tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc
    41701 catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag
    41761 tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat
    41821 tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata
    41881 aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa
    41941 tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc
    42001 ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg
    42061 ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga
    42121 tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta
    42181 gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg
    42241 atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata
    42301 gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga
    42361 ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga
    42421 aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg
    42481 caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa
    42541 gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag
    42601 atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca
    42661 aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc
    42721 gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt
    42781 tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag
    42841 caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg
    42901 atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca
    42961 gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag
    43021 aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat
    43081 tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc
    43141 aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag
    43201 tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc
    43261 aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa
    43321 aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag
    43381 aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg
    43441 aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc
    43501 gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa
    43561 agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg
    43621 tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg
    43681 tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat
    43741 tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa
    43801 gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg
    43861 cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa
    43921 aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa
    43981 caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat
    44041 gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg
    44101 tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag
    44161 atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc
    44221 aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag
    44281 aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg
    44341 ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa
    44401 tcgaacaatc tttagataga cgaaaaagca agatggccaa tgaaagaata gagctggaag
    44461 aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga
    44521 cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat
    44581 tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag
    44641 aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc
    44701 ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa
    44761 aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa
    44821 caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta
    44881 cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa
    44941 atatccagct aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa
    45001 taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta
    45061 gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag
    45121 atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga
    45181 ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg
    45241 atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca
    45301 gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc
    45361 gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag
    45421 ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg
    45481 atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa
    45541 cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac
    45601 gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc
    45661 aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg
    45721 cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag
    45781 aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta
    45841 cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc
    45901 atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag
    45961 accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt
    46021 tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc
    46081 aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg gcactttact
    46141 tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt
    46201 ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc
    46261 tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg
    46321 ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa
    46381 gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta
    46441 cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt tctgatcaaa
    46501 acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga
    46561 atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt
    46621 ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg
    46681 agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc
    46741 ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag
    46801 atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag
    46861 tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag
    46921 aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca
    46981 gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag
    47041 aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac
    47101 ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag
    47161 taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag
    47221 aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg
    47281 gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat
    47341 tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga
    47401 tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg
    47461 tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa
    47521 cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt
    47581 ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt
    47641 tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct
    47701 taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa
    47761 tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga
    47821 cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt
    47881 tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga
    47941 gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag
    48001 aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat
    48061 tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag
    48121 ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac
    48181 ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca
    48241 catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa
    48301 gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg
    48361 gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt
    48421 tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag
    48481 ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag
    48541 acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg
    48601 gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca
    48661 ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt
    48721 ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac
    48781 gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag
    48841 aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg
    48901 ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata
    48961 gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat
    49021 ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat
    49081 ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc
    49141 ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa
    49201 ggtctcttct ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg
    49261 tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa
    49321 ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa
    49381 tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat
    49441 attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg
    49501 agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt
    49561 ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata
    49621 tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa
    49681 tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat
    49741 tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa
    49801 aaattgtcta aaaaagctga ta
//
LOCUS       DAZAOG010000007        48641 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000007 DAZAOG010000000
VERSION     DAZAOG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.007"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1104
                     /locus_tag="ACHDLD_000328"
     CDS             <1..1104
                     /locus_tag="ACHDLD_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN3362091.1"
                     /translation="GVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVIL
                     QNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPL
                     LRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTI
                     HIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLY
                     GWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQ
                     EYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAE
                     LKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1202..1390
                     /locus_tag="ACHDLD_000329"
     CDS             1202..1390
                     /locus_tag="ACHDLD_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFN3362092.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1448..1801)
                     /locus_tag="ACHDLD_000330"
     CDS             complement(1448..1801)
                     /locus_tag="ACHDLD_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362093.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2332..2754
                     /locus_tag="ACHDLD_000331"
     CDS             2332..2754
                     /locus_tag="ACHDLD_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3362094.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2751..2981
                     /locus_tag="ACHDLD_000332"
     CDS             2751..2981
                     /locus_tag="ACHDLD_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362095.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3479..3679
                     /locus_tag="ACHDLD_000333"
     CDS             3479..3679
                     /locus_tag="ACHDLD_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFN3362096.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3706..4899
                     /locus_tag="ACHDLD_000334"
     CDS             3706..4899
                     /locus_tag="ACHDLD_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3362097.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4967..6532)
                     /gene="guaA"
                     /locus_tag="ACHDLD_000335"
     CDS             complement(4967..6532)
                     /gene="guaA"
                     /locus_tag="ACHDLD_000335"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN3362098.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6591..7514)
                     /gene="coaA"
                     /locus_tag="ACHDLD_000336"
     CDS             complement(6591..7514)
                     /gene="coaA"
                     /locus_tag="ACHDLD_000336"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN3362099.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7710..8315
                     /locus_tag="ACHDLD_000337"
     CDS             7710..8315
                     /locus_tag="ACHDLD_000337"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3362100.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8623..9639
                     /locus_tag="ACHDLD_000338"
     CDS             8623..9639
                     /locus_tag="ACHDLD_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362101.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9780..11081
                     /locus_tag="ACHDLD_000339"
     CDS             9780..11081
                     /locus_tag="ACHDLD_000339"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN3362102.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11127..11789
                     /gene="deoC"
                     /locus_tag="ACHDLD_000340"
     CDS             11127..11789
                     /gene="deoC"
                     /locus_tag="ACHDLD_000340"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN3362103.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11810..12208
                     /locus_tag="ACHDLD_000341"
     CDS             11810..12208
                     /locus_tag="ACHDLD_000341"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN3362104.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12274..13356
                     /locus_tag="ACHDLD_000342"
     CDS             12274..13356
                     /locus_tag="ACHDLD_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3362105.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13568..15130
                     /locus_tag="ACHDLD_000343"
     CDS             13568..15130
                     /locus_tag="ACHDLD_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362106.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15123..16256
                     /locus_tag="ACHDLD_000344"
     CDS             15123..16256
                     /locus_tag="ACHDLD_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362107.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16256..17212
                     /locus_tag="ACHDLD_000345"
     CDS             16256..17212
                     /locus_tag="ACHDLD_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362108.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17311..18477
                     /gene="deoB"
                     /locus_tag="ACHDLD_000346"
     CDS             17311..18477
                     /gene="deoB"
                     /locus_tag="ACHDLD_000346"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN3362109.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18501..19319
                     /locus_tag="ACHDLD_000347"
     CDS             18501..19319
                     /locus_tag="ACHDLD_000347"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN3362110.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19336..20043
                     /gene="deoD"
                     /locus_tag="ACHDLD_000348"
     CDS             19336..20043
                     /gene="deoD"
                     /locus_tag="ACHDLD_000348"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN3362111.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20341..21027)
                     /gene="gpmA"
                     /locus_tag="ACHDLD_000349"
     CDS             complement(20341..21027)
                     /gene="gpmA"
                     /locus_tag="ACHDLD_000349"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN3362112.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21199..21780)
                     /locus_tag="ACHDLD_000350"
     CDS             complement(21199..21780)
                     /locus_tag="ACHDLD_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3362113.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21913..22596)
                     /gene="rpiA"
                     /locus_tag="ACHDLD_000351"
     CDS             complement(21913..22596)
                     /gene="rpiA"
                     /locus_tag="ACHDLD_000351"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN3362114.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22915..23328
                     /gene="rpsL"
                     /locus_tag="ACHDLD_000352"
     CDS             22915..23328
                     /gene="rpsL"
                     /locus_tag="ACHDLD_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN3362115.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23412..23882
                     /gene="rpsG"
                     /locus_tag="ACHDLD_000353"
     CDS             23412..23882
                     /gene="rpsG"
                     /locus_tag="ACHDLD_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN3362116.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23976..26063
                     /gene="fusA"
                     /locus_tag="ACHDLD_000354"
     CDS             23976..26063
                     /gene="fusA"
                     /locus_tag="ACHDLD_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN3362117.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26235..27422
                     /gene="tuf"
                     /locus_tag="ACHDLD_000355"
     CDS             26235..27422
                     /gene="tuf"
                     /locus_tag="ACHDLD_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN3362118.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27829..28137
                     /gene="rpsJ"
                     /locus_tag="ACHDLD_000356"
     CDS             27829..28137
                     /gene="rpsJ"
                     /locus_tag="ACHDLD_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN3362119.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28168..28797
                     /gene="rplC"
                     /locus_tag="ACHDLD_000357"
     CDS             28168..28797
                     /gene="rplC"
                     /locus_tag="ACHDLD_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN3362120.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28825..29448
                     /gene="rplD"
                     /locus_tag="ACHDLD_000358"
     CDS             28825..29448
                     /gene="rplD"
                     /locus_tag="ACHDLD_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN3362121.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29448..29738
                     /gene="rplW"
                     /locus_tag="ACHDLD_000359"
     CDS             29448..29738
                     /gene="rplW"
                     /locus_tag="ACHDLD_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN3362122.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29777..30610
                     /gene="rplB"
                     /locus_tag="ACHDLD_000360"
     CDS             29777..30610
                     /gene="rplB"
                     /locus_tag="ACHDLD_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN3362123.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30653..30931
                     /gene="rpsS"
                     /locus_tag="ACHDLD_000361"
     CDS             30653..30931
                     /gene="rpsS"
                     /locus_tag="ACHDLD_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN3362124.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30954..31301
                     /gene="rplV"
                     /locus_tag="ACHDLD_000362"
     CDS             30954..31301
                     /gene="rplV"
                     /locus_tag="ACHDLD_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN3362125.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31315..31971
                     /gene="rpsC"
                     /locus_tag="ACHDLD_000363"
     CDS             31315..31971
                     /gene="rpsC"
                     /locus_tag="ACHDLD_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN3362126.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31974..32408
                     /gene="rplP"
                     /locus_tag="ACHDLD_000364"
     CDS             31974..32408
                     /gene="rplP"
                     /locus_tag="ACHDLD_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN3362127.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32398..32586
                     /gene="rpmC"
                     /locus_tag="ACHDLD_000365"
     CDS             32398..32586
                     /gene="rpmC"
                     /locus_tag="ACHDLD_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN3362128.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32611..32877
                     /gene="rpsQ"
                     /locus_tag="ACHDLD_000366"
     CDS             32611..32877
                     /gene="rpsQ"
                     /locus_tag="ACHDLD_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN3362129.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32935..33303
                     /gene="rplN"
                     /locus_tag="ACHDLD_000367"
     CDS             32935..33303
                     /gene="rplN"
                     /locus_tag="ACHDLD_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN3362130.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33340..33648
                     /gene="rplX"
                     /locus_tag="ACHDLD_000368"
     CDS             33340..33648
                     /gene="rplX"
                     /locus_tag="ACHDLD_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN3362131.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33676..34215
                     /gene="rplE"
                     /locus_tag="ACHDLD_000369"
     CDS             33676..34215
                     /gene="rplE"
                     /locus_tag="ACHDLD_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN3362132.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34234..34419
                     /locus_tag="ACHDLD_000370"
     CDS             34234..34419
                     /locus_tag="ACHDLD_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN3362133.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34455..34853
                     /gene="rpsH"
                     /locus_tag="ACHDLD_000371"
     CDS             34455..34853
                     /gene="rpsH"
                     /locus_tag="ACHDLD_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN3362134.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34885..35421
                     /gene="rplF"
                     /locus_tag="ACHDLD_000372"
     CDS             34885..35421
                     /gene="rplF"
                     /locus_tag="ACHDLD_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN3362135.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35582..35938
                     /gene="rplR"
                     /locus_tag="ACHDLD_000373"
     CDS             35582..35938
                     /gene="rplR"
                     /locus_tag="ACHDLD_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN3362136.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35959..36459
                     /gene="rpsE"
                     /locus_tag="ACHDLD_000374"
     CDS             35959..36459
                     /gene="rpsE"
                     /locus_tag="ACHDLD_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN3362137.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36474..36653
                     /gene="rpmD"
                     /locus_tag="ACHDLD_000375"
     CDS             36474..36653
                     /gene="rpmD"
                     /locus_tag="ACHDLD_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN3362138.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36697..37137
                     /gene="rplO"
                     /locus_tag="ACHDLD_000376"
     CDS             36697..37137
                     /gene="rplO"
                     /locus_tag="ACHDLD_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN3362139.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37137..38432
                     /gene="secY"
                     /locus_tag="ACHDLD_000377"
     CDS             37137..38432
                     /gene="secY"
                     /locus_tag="ACHDLD_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN3362140.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38494..39141
                     /locus_tag="ACHDLD_000378"
     CDS             38494..39141
                     /locus_tag="ACHDLD_000378"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN3362141.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39332..39550
                     /gene="infA"
                     /locus_tag="ACHDLD_000379"
     CDS             39332..39550
                     /gene="infA"
                     /locus_tag="ACHDLD_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN3362142.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39583..39699
                     /gene="rpmJ"
                     /locus_tag="ACHDLD_000380"
     CDS             39583..39699
                     /gene="rpmJ"
                     /locus_tag="ACHDLD_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN3362143.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39717..40082
                     /gene="rpsM"
                     /locus_tag="ACHDLD_000381"
     CDS             39717..40082
                     /gene="rpsM"
                     /locus_tag="ACHDLD_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN3362144.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40110..40499
                     /gene="rpsK"
                     /locus_tag="ACHDLD_000382"
     CDS             40110..40499
                     /gene="rpsK"
                     /locus_tag="ACHDLD_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN3362145.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40579..41517
                     /locus_tag="ACHDLD_000383"
     CDS             40579..41517
                     /locus_tag="ACHDLD_000383"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN3362146.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41545..41925
                     /gene="rplQ"
                     /locus_tag="ACHDLD_000384"
     CDS             41545..41925
                     /gene="rplQ"
                     /locus_tag="ACHDLD_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN3362147.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42197..43036
                     /locus_tag="ACHDLD_000385"
     CDS             42197..43036
                     /locus_tag="ACHDLD_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3362148.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMVEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            43012..43881
                     /locus_tag="ACHDLD_000386"
     CDS             43012..43881
                     /locus_tag="ACHDLD_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3362149.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43871..44668
                     /locus_tag="ACHDLD_000387"
     CDS             43871..44668
                     /locus_tag="ACHDLD_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN3362150.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44871..45617
                     /gene="truA"
                     /locus_tag="ACHDLD_000388"
     CDS             44871..45617
                     /gene="truA"
                     /locus_tag="ACHDLD_000388"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN3362151.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45950..48619)
                     /locus_tag="ACHDLD_000389"
     CDS             complement(45950..48619)
                     /locus_tag="ACHDLD_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN3362152.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15700 a         8914 c        10391 g        13636 t
ORIGIN      
        1 ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca
       61 gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta ttctggagaa
      121 attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta
      181 ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg
      241 agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat
      301 ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg
      361 aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa
      421 ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt
      481 cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt
      541 ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca
      601 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
      661 aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact
      721 ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga
      781 acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga atatctttca
      841 cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat
      901 aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat
      961 ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt
     1021 accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga
     1081 tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat
     1141 taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt
     1201 tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat
     1261 acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac
     1321 tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca
     1381 gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt
     1441 ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta
     1501 aggatagttc tttgtctgta aagctatcca tgtatttttc tacctgtaac cttcgagtgc
     1561 ttttaatcaa attatctgta ggtaagaaaa attcatcaat agatacatgg agtaatgata
     1621 caagatcata cagcacttgt gtacttgggt gttgtccttt gttttctata tttgttaaat
     1681 aacgtgggtc aatttcaatc attgttccca cttgttcacg agtgagaccc cgtttcattc
     1741 gagcctcttt aatcgctaaa ccaacggctc taaaatcgta tttatcttct tttttacgca
     1801 tactaaatca cctctataca ttttactgtt cctatataac cataaacagg tatagaaaaa
     1861 catgttatat agtttatgag ttcctataga acactgtgga atttattaat gcgatgaatc
     1921 caatttcaaa tagaaaggtc gctgatttta tttacctata caactttata aaatttagtc
     1981 atactcctag aaagtagata aaattggcga cttaatggag ggaaaagtcg ccatgagaaa
     2041 gataatctgt catctagttc tcttaattaa gttcttatca aaaaaactta tccacctgta
     2101 ctggatagat atgaccatca atgtggtttt gcagattaca tagatagaaa aatcaaagca
     2161 tatgaacaga gaaaacaatc tgtttatgtg cttttttggt cacttaataa atatctttta
     2221 ggatttctct tcatttatgc aacaaatccc ccgttcgtgt cggggtttag tatgaaggga
     2281 gataaagaag cacatgccac tccctttcac gacagaaagg gggtgaaaag catgaaacca
     2341 tcttcttttc aagaaaccat agaaaatcag tttgactata tctgcaaaca agttattgaa
     2401 gatgaacgaa aagattactt caaacatcta gacagactat cagcaaaaga agtctcgttt
     2461 tctgatatag ataactacct tgtcaactct ttttctactg ttgatacgta cgtcactgat
     2521 ttccaactat ttaaattatt tggattaaga atcaatgttg aaagtgattt attaagtgaa
     2581 gccctgcgtc atctttcaga gaaaaaacgg aacattattt tacttcatta ctttatggat
     2641 atgagtgatg tggagattgc ggagttacta aatttgaatc gttcgactgt ttatcgtcat
     2701 cgaataagtg gactagccat gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag
     2761 cttatccaag ggttcccttc cccctcattg taaaggcgac agatggcgac gtggaagcaa
     2821 ttaatcagat tgttaaacat tatcgagggt atacctcaaa acgttcatta cgtcgcatga
     2881 cagacgaata tggaaattct catatggtta ttgatgaaac cctacgtggc agaatggaaa
     2941 cgaaactcat ctctaagatt ttatcgttcg aaattaaata gtcccatttt ttcgtggaag
     3001 cgtgtcgaat cgtatccacg cttcccgatt tgggaggttt gttattccat caaagcatat
     3061 tgtgtgttca gtaggttttg ataggctaac aagccaaacg ttctttgaca accaaataaa
     3121 ggcaatcgaa tacgtttcgg taagaaaaga gctgatgaac taacacgcca tgacctacaa
     3181 aattagtgag cgattcatgt tagtaattcg aaagtaatct ttagcaggat tgctgccatg
     3241 actttcttat cgtgataatg atactcccgt atagccatag tccgagcgtt agaagcgtca
     3301 caggcaatgg gtacggctac atgagaacca tgcagaagtg aaattcctgt gagctttgct
     3361 agagctgttc gattgctaag atattcttct cttgtcagtg tattattata aaactatttg
     3421 atataattat aggtgaatat tggagttttc ctagcctata attgggaaag gagtaataat
     3481 gataacggtc acaaaaaagg atttagttga gctaggatat ggaacttctt tttcagcaga
     3541 catcataaaa aaagcaaaag aattgatgat tacaaaaggg catatctact atcaatctcg
     3601 aaagctagat agagtccctc gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa
     3661 tgaaaacagg tctgagtagt ttttgcacag taaggagtaa aaactatggt taaagaccca
     3721 ataaaaaaag caaagaatgg aacctattat tttcgtgcaa atctaggtta tgattctaaa
     3781 gggaaaaaaa ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc aagagaagaa
     3841 tattcgaaac tgctattgat gaaaccagaa gaattaagtg agaataagga taaaatgacg
     3901 tttaaacatt atatctttga aattttcctt ccatggtata agacacaggt aaagctcaga
     3961 acatatgaaa atcgcttacc aactattaag aagcatttct cttattttga taaaatggct
     4021 gtttccgata ttgagcctat tgatgtgcaa aattggcaac taaaattatc aaagaagtgt
     4081 aagtcttctt atgtaagagc tgttcaagga ctattttcag ttgctatgga tcgagcaatt
     4141 gtccttggga taaccaccac aaatccatct aagattattg gcaatgtgaa aaagcaaaag
     4201 tctaaaattg aattttggac aaaagaagaa tttgaaaaag taatctctct aatttacaaa
     4261 gaagattact atcaacactt tttatttatc tctctgtggt ttttatttat gactggaatg
     4321 cgaattggtg aagctactgc cattcaatgg gaagatattg attttgactc aggagtacta
     4381 accatcaaca aaacactcta ttacaagaat caatcgaatt atagatttgt tgaaccaaaa
     4441 acaaaggcga gcgttcgtca tattgtatta gataaatgca cattaactta tttatccgaa
     4501 tggaaggaag tacaacaaaa tctaattcag acagactttg taatgagcta taacggtatt
     4561 cctacccaaa aacatacact atcatatgct attgaacgct atgctaagct ggctggtatc
     4621 catcgaatca gaatccatgg gttacggcat tctcatgctt ccttgttaat cagtatggga
     4681 gaaaatccac ttatcattaa agatcgtcta ggacacgaag atattgagac gactttagga
     4741 acgtatggac atctttatcc taacagtaat ttcgaggtag ctaacaaatt aaatggagcc
     4801 atttccttta aagaagcaaa acaaaattat gatacttcac caaagaatca atttactgta
     4861 gactacttgc gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga
     4921 agagccagaa acccttggta taagaggatt tctagctctc ctacaactat tcccactcca
     4981 ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt
     5041 ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg
     5101 tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt
     5161 cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac
     5221 ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt
     5281 ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg
     5341 gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt
     5401 ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac
     5461 ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag
     5521 tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt
     5581 cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag
     5641 atacaccttt caacttgtct aagaagcgat ctcttgcatc gactttgatg atgttcaagc
     5701 cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt
     5761 ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga
     5821 cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac
     5881 ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac
     5941 aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg
     6001 catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat
     6061 ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc
     6121 tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta
     6181 cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt
     6241 tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga
     6301 tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa
     6361 tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt
     6421 cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt
     6481 agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta
     6541 cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt
     6601 cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt
     6661 cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct
     6721 tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct
     6781 ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc
     6841 agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc
     6901 tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg
     6961 atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt
     7021 atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa
     7081 ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa
     7141 agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc
     7201 gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata
     7261 ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat
     7321 tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca
     7381 tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa
     7441 ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc
     7501 attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa
     7561 ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat
     7621 agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg
     7681 tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag
     7741 atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg
     7801 taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg
     7861 atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt
     7921 atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca
     7981 ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg
     8041 ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg
     8101 taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat
     8161 atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc
     8221 cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata
     8281 aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg
     8341 aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt
     8401 ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt
     8461 ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata
     8521 aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt
     8581 tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga
     8641 gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa
     8701 ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat
     8761 tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg
     8821 atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat
     8881 ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag
     8941 atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca
     9001 aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt
     9061 ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag
     9121 ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg
     9181 aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg
     9241 aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa
     9301 ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc
     9361 gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc
     9421 ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg
     9481 caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg
     9541 atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta
     9601 attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg
     9661 taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag
     9721 tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa
     9781 tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa
     9841 tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat
     9901 tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg
     9961 cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata
    10021 agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta
    10081 gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac
    10141 tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac
    10201 gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg
    10261 acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg
    10321 ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga
    10381 ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca
    10441 tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag
    10501 aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac
    10561 aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg
    10621 ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg
    10681 aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag
    10741 cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta
    10801 aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc
    10861 taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc
    10921 ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg
    10981 acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg
    11041 caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta
    11101 ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt
    11161 tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc
    11221 atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag
    11281 gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga
    11341 caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca
    11401 tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag
    11461 ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag
    11521 atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca
    11581 aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg
    11641 aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag
    11701 caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg
    11761 tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact
    11821 caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc
    11881 ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa
    11941 cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc
    12001 agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc
    12061 tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc
    12121 ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc
    12181 ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa
    12241 aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta
    12301 ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact
    12361 gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca
    12421 ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg
    12481 aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa
    12541 tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt
    12601 attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac
    12661 tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa
    12721 gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta
    12781 gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa
    12841 gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat
    12901 gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa
    12961 aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca
    13021 aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa
    13081 tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca
    13141 ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc
    13201 cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga
    13261 caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt
    13321 gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga
    13381 cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc
    13441 cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa
    13501 cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga
    13561 aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg
    13621 aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt
    13681 gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga
    13741 gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa
    13801 agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac
    13861 tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa
    13921 aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga
    13981 tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact
    14041 ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga
    14101 aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt
    14161 gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg
    14221 tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat
    14281 gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt
    14341 cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa
    14401 cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg
    14461 gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa
    14521 gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg
    14581 acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa
    14641 tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc
    14701 agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga
    14761 acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga
    14821 agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg
    14881 tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt
    14941 actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca
    15001 tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct
    15061 attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc
    15121 acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt
    15181 taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt
    15241 attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc
    15301 aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt
    15361 tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac
    15421 ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct
    15481 tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg
    15541 aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac
    15601 atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa
    15661 atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag
    15721 gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt
    15781 taggatttga aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga
    15841 gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg
    15901 gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta
    15961 ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt
    16021 tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag
    16081 gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca
    16141 acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg
    16201 ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc
    16261 gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat
    16321 tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga
    16381 gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc
    16441 acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt
    16501 ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac
    16561 agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa
    16621 aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga
    16681 tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat
    16741 tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg
    16801 ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg
    16861 ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca
    16921 gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc
    16981 tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg
    17041 ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc
    17101 agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg
    17161 gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag
    17221 cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc
    17281 aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg
    17341 gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat
    17401 acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt
    17461 ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg
    17521 acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg
    17581 ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc
    17641 aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca
    17701 gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact
    17761 tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta
    17821 tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga
    17881 atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat
    17941 gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag
    18001 gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct
    18061 gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat
    18121 ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg
    18181 gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt
    18241 gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg
    18301 tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg
    18361 aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag
    18421 aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct
    18481 ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt
    18541 ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag
    18601 gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct
    18661 ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca
    18721 agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag
    18781 tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg
    18841 catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata
    18901 tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat
    18961 ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag
    19021 aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg
    19081 aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga
    19141 ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta
    19201 ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa
    19261 ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat
    19321 aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag
    19381 attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat
    19441 ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa
    19501 cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa
    19561 ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca
    19621 aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg
    19681 atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg
    19741 aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg
    19801 gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt
    19861 ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag
    19921 acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca
    19981 gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa
    20041 taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag
    20101 ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt
    20161 ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa
    20221 cagcttttca tgtttgatga atattgtgac ttgtgaccga gaagttggca ggagaaaacc
    20281 gtttatacga aaaaagggac tgtgacaaat cgtgtcacag tccctttttt atagtgtttt
    20341 ttataggtag tattttttag caactgttaa gtcatcattt aattcataaa caagtggttg
    20401 acctgttgga atttcaagat ccatgatgtc ttcgtcagag ataccttcga tatgtttagc
    20461 tagggcgcgt aatgagttac catgtgcagc tactaaaaca gttttgttgt ctagtaatgc
    20521 tggagcaatt tcatcttgcc agaatggcaa agcgcgttct aatgtcactt tcaagttttc
    20581 tccaccagga atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc
    20641 ttcgtcagtt tcttccatta atggaggaag agtatcgtaa gaacgacgcc agatgtgcac
    20701 ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc aaaccttgta atttaccata
    20761 gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc caaagttgat ctgaatattc
    20821 taagatcaag ttacatgttt tgattgcacg agttaaaaca gaagtgtaag caacatcaaa
    20881 ttcgattcca gcttctttga ttttgcgtcc gccttctttt gcttcttcga ttccttctgg
    20941 tgctagattt acatcagccc aaccagtaaa ttggttcaat gcattccatt cactaagacc
    21001 gtgacgagaa aaaactaatt ttggcatgtt tagctctcct tttaagatag gtttatattt
    21061 ctgaaatttt cagttctgtt ttattgtaca attttttgga agagatttcc atcgaaaaat
    21121 tagataagat tctatgagaa aacaagtaga gaatataaaa aaacgaacag tgcccacgca
    21181 actgttcgtt tttaggtatt agatgcataa ttgctcgatt tctttttcag gtaaattgat
    21241 atatttataa catgttccaa ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc
    21301 ttttttttca taatagagac gtcggatttt tttgatcgtt tttgcatcga tttttggtct
    21361 tccgccgatt tttccttttt tacgagcttc atctaatcca actaatgtac gttctttgat
    21421 caatgcgcac tccatgtttg ctaggttttc cattaattgg aaatagattt ttcccatttc
    21481 gcgcgtatcg attccttcat caagactaat caagtgcagc ccgcgatctt tgaataattg
    21541 cgtgagttct gttaattgcc gcgtagattt gcctaatcga tgaagttggc aaatgactag
    21601 tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa tgaacagcag tagaatcatt
    21661 ttgttccact tcatatgtct catggaaaat ttcttgacaa ccaaagtcag ataattgatc
    21721 aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa taattttcat
    21781 ggtgattcct ttctagcagt gattttctaa ctggatataa cagtaaaaat gtagcataaa
    21841 gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt ctttttttat tggatgatca
    21901 agcagttttt ctttatttac gacggattgc gtcaagaatc tcaacgcctt tttctgagcc
    21961 gacaactact ttagaaacca tgtttaggaa aagcccatgt tctactaccc caacaagttc
    22021 atctaaatag gtagctaagc tttctggctg ttcgatgact tctagatgca gatcaatgat
    22081 gtaatgacca ccatctgtgg ttaagacttc tccgttttcg tctgtacgca agacgggttg
    22141 gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag ccataaggga tcacttcaac
    22201 tgggagtggg aacttcccaa gtttatcgac taatttagaa gaatcaacga tccaaatcgt
    22261 ttcttttgag tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat
    22321 cccttgaaaa tcagcactga tttcatcagc accgtctatt gtcaaatcga ctacggggac
    22381 ttcatcaata ctctttaacg gtattcctaa tttttccgct tgttcttttg tcgcatttga
    22441 agtagtaact cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa tctcttctac
    22501 catataatag gcggttgaac cggtcccgag acctacgatc atcccgtcct ttacgaattt
    22561 cgcggcttca atgccagcta attgttttaa attcatcttt gcgtccacct ttttttagtt
    22621 tgagcatcgt gctttcctca ctattttagc gtgatttacg gtaaaaagat accattcttt
    22681 tttattccaa taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt
    22741 cgtgatattc taacaaaggt gctttatcta caggtttctg tgaagaaacg tgctgactgt
    22801 ttatcaaagc atatttgata aggatgcaga cattgcattt attttttatc acaaaaaaag
    22861 aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct
    22921 acaattaacc aattagtacg taaacctcgt aaatcaaagg tggaaaaatc taattcacct
    22981 gctttgaaca aaggatataa cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa
    23041 aaacgtgggg tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta
    23101 cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag ttactgctta tatcccaggg
    23161 attggtcata acttacaaga acacagtgtt gtattattac gcggtggtcg tgtaaaagac
    23221 ttaccagggg tacgttacca tatcgttcgc ggtgctttag atactgctgg tgttaacgac
    23281 cgtaaacaaa gccgttctaa atacggaact aaaaaaccta aagcttaatt ttaattatta
    23341 aacaaatgaa acttgcttct actatataga agtaattact atgaacaata tttcggaagg
    23401 aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta
    23461 tttataactc aaaattagta actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg
    23521 ggattgcagc taacattatc tataatgcct ttgacttgat caaagaatct acaggtaacg
    23581 atccattaga agtgtttgaa caagcaatga aaaacgttca acctgttctt gaagttaaag
    23641 cacgtcgtgt tgggggttca aactaccaag taccagttga agttcgccct gaacgccgcg
    23701 ttactctagg tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg
    23761 aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa
    23821 aacgtgaaga cactcataaa atggctgatg ccaaccgtgc gtttgctcac tatcgctggt
    23881 aagattctct ctgtttggtc ggcttttata agtcgacctc agatagtgtc cttattttta
    23941 gcaactagat agtaaagaga ggagaaacaa ggagaatgac cagagaattt tctttacaaa
    24001 acactcgtaa tatcggtatc atggcccaca tcgatgctgg taaaacgact gctactgaac
    24061 gtatcttgta ctatactggt aaaatccata aaattggtga aactcatgaa ggagcttcac
    24121 aaatggactg gatggaacaa gaacaagaac gtggtattac gatcacttct gctgctacta
    24181 cagcacaatg gaagggtcac cgaatcaaca tcatcgacac accaggacac gtagacttca
    24241 cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac
    24301 aatcaggtgt tgaacctcaa actgaaacag tttggcgtca agcaacaact tatggtgtac
    24361 ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc tgacttctta tattcagtat
    24421 caactttaca tgaccgtttg caagcaaacg ctcacccaat ccaattacca attggtgcgg
    24481 aagacgactt cacaggaatt atcgaccttg taacaatgaa ggctgaaatg tatacaaacg
    24541 atcttggaac agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag
    24601 aatggcgcga aaaattggtt gaagctgtag ctgaaactga tgaagaactt actttgaaat
    24661 acttagaagg tgaagaaatc actgaagctg agttaaaaga aggtatccgc cgcgcaacag
    24721 ttaacgtaga attctaccca gtactatgtg gttcagcatt taagaacaaa ggggttcaat
    24781 tactattgga cgctgttctt gattacttgc catcaccgtt agatatccca gctatcaaag
    24841 ggattgaccc taaaactgat gaagaagtag aacgtcctgc agacgacagc gctcctttct
    24901 cagctttagc atttaaagtt atgactgacc cattcgtagg tcgtttaact ttcttccgtg
    24961 tgtactctgg tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg
    25021 aacgtgttgg tcgtatccta cagatgcatg ccaactctcg ttcagaaatc tctgaagttt
    25081 acgctggaga tatcgctgct gctgttggtt tgaaagacac aactactggg gacacactat
    25141 gtgatgaaaa gagtctagtt attcttgaat ccatggaatt cccagaacca gttatccaag
    25201 tcgctattga acctaaatca aaagctgacc aagataaaat gggtgtggct ttacaaaaac
    25261 tatctgaaga agatcctact ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca
    25321 tcgctggtat gggtgaactt cacttagata tcatcgttga ccgtatgcgt cgtgaattca
    25381 aagttgaagc taacgtaggt gcgcctcaag tatcttaccg tgaaactttc cgtgctggca
    25441 ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat
    25501 ggattgaatt tacaccaaat gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg
    25561 gtggggttgt tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa gatgctatgg
    25621 aaaatggtgt cttagctggc tatccattag tagatatcaa agctaaactt tacgatggtt
    25681 cataccatga tgtcgattca aatgaaactg ccttccgtgt tgctgcttct atggcattac
    25741 gtgcagcagc taagaaagct aatccagtaa tccttgaacc aatcatggct gtagaagttg
    25801 ttatccctga agattattta ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg
    25861 ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg gttccacttg
    25921 ctgaaatgtt tggttatgct acaactcttc gttctgctac tcaaggtcgt ggtacattta
    25981 caatgacatt tgaccactac gaagatgtac caaaatcagt acaagaagaa atcattaaga
    26041 aaaacggcgg aaaagctgaa taatttctta aaaccggata gtcctgcaaa gagattatcc
    26101 aaagagacta tatttttaat atagtttcgt gtaaaataat tatcgatgat atgggcatgt
    26161 ggtgtacctt gtgtacatca tatacctatc agacaaataa aaaatatatt gtatttttag
    26221 gaggacattt taaaatggca aaagaaaaat ttgaccgttc taaaccacac gttaacattg
    26281 gtactatcgg acacgttgac catggtaaaa ctacattaac agctgcaatc acaactgtac
    26341 tatctaagaa aaacggcggc caagctatgg cttacgatca aatcgatggt gctccagaag
    26401 aacgcgaacg cggaatcaca atctcaacag ctcacgttga gtatgaaaca gatacacgcc
    26461 actacgctca cgttgactgc ccaggacacg cggattacgt taaaaacatg atcactggtg
    26521 ctgctcaaat ggacggagct atcttggtag tttctgctgc tgacggccca atgcctcaaa
    26581 ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga
    26641 acaaagtaga catggttgat gacgaagaat tactagaatt agttgaaatg gaagttcgtg
    26701 acctattaac agaatacgaa ttccctggtg acgatgttcc tgtagttgct ggatcagctt
    26761 tgaaagctct agaaggcgac gcttcatacg aagaaaaaat tcttgaatta atggctgcag
    26821 ttgacgaata catcccaact ccagaacgtg acaacgacaa accattcatg atgccagttg
    26881 aagacgtgtt ctcaattact ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac
    26941 aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa
    27001 cagttactgg tgttgaaatg ttccgtaaat tgttagacta cgctgaagct ggagacaaca
    27061 ttggtgcttt actacgtggt gttgcacgtg aagacatcca acgtggacaa gttttagcta
    27121 aaccaggtac aatcacacct catacaaaat tctctgcaga agtatacgtg ttgacaaaag
    27181 aagaaggtgg acgtcatact ccattcttca ctaactaccg tccacaattc tacttccgta
    27241 caactgacgt aacaggtgtt gttgaattac cagaaggaac tgaaatggtt atgcctggcg
    27301 acaacgtaac aatggaagtt gaattaatcc acccaatcgc tatcgaaaac ggtactaaat
    27361 tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa atcaaagctt
    27421 aattcactta agttttaaag taaagcaaca tttattcgga cgtaatcgct cggaacgaag
    27481 gtcttaggat ctttgttccg agctttttta tttattgcag gatcaggaaa aaagacagga
    27541 gtattataaa tagaagaaac acgaaataaa agagtcagta ttttctaaat gaaactttat
    27601 gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt gagtttatat atattataat
    27661 gacaaaggtg cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct
    27721 aagaaacgag aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt
    27781 ttcgtggagc tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa
    27841 aaaattcgta tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa
    27901 atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact
    27961 gaacgcagtc tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa
    28021 ttcgaaatgc gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt
    28081 gatgctttga tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac
    28141 taaaacaaaa ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga
    28201 atgacacaaa tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact
    28261 ccaaacgtag tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt
    28321 ggttaccaag atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa
    28381 gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat
    28441 gaagtaggaa aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca
    28501 ggtactacga aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga
    28561 ccaatggctc acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct
    28621 aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc
    28681 caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat
    28741 gttcctggag ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc taaataattg
    28801 taggaagaaa ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc
    28861 caaaacggtg aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt
    28921 gtttacgatg caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa
    28981 aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt
    29041 gcacgtcaag gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca
    29101 acaccacgtt cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct
    29161 gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac
    29221 gcaccaaaaa caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta
    29281 ttggttgttc tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac
    29341 gtttctgtag taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg
    29401 ttagcaacac aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag
    29461 acgtaatcaa acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat
    29521 acacttttga agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg
    29581 cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta
    29641 tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag
    29701 attcaaaaga aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa
    29761 attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa
    29821 catgacaggt tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca
    29881 accattgaaa aaccatgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg
    29941 tggcggacac aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt
    30001 tgcaactgtt caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca
    30061 ctatgaagat ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa
    30121 acttgtttca ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat
    30181 tccagttggt actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat
    30241 ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg
    30301 cttgaactct ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt
    30361 aggtaacgaa caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg
    30421 taaacgccca actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg
    30481 tgaaggtaaa gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct
    30541 tggttacaaa actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa
    30601 aaataaataa ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg
    30661 tagtttaaag aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc
    30721 aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag
    30781 ttttgttgga tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca
    30841 agaagacatg gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca
    30901 tgctgctgac gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag
    30961 aacaaatcac atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc
    31021 gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat
    31081 tcacaccaaa caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg
    31141 cagaaaataa ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg
    31201 aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca
    31261 aacgtacaag tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt
    31321 caaaaagtac atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg
    31381 tatgctgaaa aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt
    31441 tcaactaaac ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc
    31501 gtaaacattt caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa
    31561 gtcgaaagcc taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc
    31621 gtggaaatca aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa
    31681 ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt
    31741 gctggcgctc aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct
    31801 cgttcagaag gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat
    31861 tacgcatggg aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat
    31921 cgtggagaaa ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag
    31981 tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag
    32041 gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca
    32101 caaaccgcca aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga
    32161 aagtatggat taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta
    32221 tgggttctgg taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca
    32281 tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc
    32341 acaaattacc aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg
    32401 aaggttaaag aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta
    32461 aaagaagaat tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca
    32521 cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct
    32581 aagtaattat ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt
    32641 tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca
    32701 aagaaaaacc accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat
    32761 gatgaaaaca acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta
    32821 tcagctacaa aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa
    32881 cacgagattt tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc
    32941 caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc
    33001 aaagtccttg gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg
    33061 gttaaacaag caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc
    33121 gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat
    33181 gctgcagtga ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt
    33241 gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta
    33301 taatcttgag acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat
    33361 aaagttaaag ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc
    33421 ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct
    33481 tctcaagctg ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac
    33541 gtgatggtgg ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc
    33601 aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa
    33661 ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca
    33721 ttgatggaaa aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc
    33781 atcaacatgg gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa
    33841 gaattaactt tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct
    33901 ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga
    33961 atgtacgaat tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat
    34021 ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa
    34081 ttgatcttcc cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt
    34141 attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg
    34201 ccattccaaa aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa
    34261 aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt
    34321 ccacattcag tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat
    34381 aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag
    34441 gaggtaaaag atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta
    34501 acgcaaacat ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca
    34561 tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca
    34621 aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa
    34681 acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa
    34741 aagtattgaa cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata
    34801 aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta
    34861 gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct
    34921 gaaggagtaa cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa
    34981 ttgactcgta ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc
    35041 actcgtccaa acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc
    35101 aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt
    35161 gggtaccgtg cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca
    35221 gtagaaatca caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt
    35281 gttaaaggtg cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt
    35341 cctccagaac cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa
    35401 gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa
    35461 aggcatttgg ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta
    35521 tttttcgaaa cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat
    35581 tgtgattaca aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa
    35641 caatatctct ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat
    35701 ctacgctcaa attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga
    35761 taaagaaatt tcaggtggaa caaaaacaga agcagcacaa gctgtcggta aactagttgc
    35821 tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg gataccttta
    35881 ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa
    35941 gaaggaggaa taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc
    36001 gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct
    36061 gctttagttg ttgtcggtga caaaaacgga cacgtagggt ttggtacagg taaagctcaa
    36121 gaagttcctg aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta
    36181 cctatggttg gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc
    36241 ctaatgaaac ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta
    36301 ttagaattag ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc
    36361 aacgttgttc gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca
    36421 gaacttcgcg gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg
    36481 aattaaaagt tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag
    36541 ttaaagcgtt aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa
    36601 ttaaaggcat gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa
    36661 aaatcataac tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct
    36721 gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga
    36781 aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta
    36841 ggatttgaag ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac
    36901 gtgaaccgta aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga
    36961 gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga
    37021 atcaaagtat tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc
    37081 tctaaagcag ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt
    37141 ttagactatt aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg
    37201 tatttgtgtt gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg
    37261 cttcattgca aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta
    37321 gtgcgatgca aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta
    37381 tcgtcattca gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag
    37441 gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat
    37501 ttatccaatc tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga
    37561 ataatcctaa cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt
    37621 ttgttacttg gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga
    37681 tcatttttgc aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt
    37741 actttataaa tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac
    37801 tagtagcggt cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga
    37861 tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga
    37921 aagtaaatgc agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa
    37981 atgctgtttt acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga
    38041 tggagatctt taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg
    38101 ccttcacttt cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc
    38161 aaaagcaagg aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct
    38221 caagattact gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc
    38281 ttccaatcat tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa
    38341 gcttactgat cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc
    38401 tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca
    38461 cacgttctca cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca
    38521 ggcgcaggta aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc
    38581 tcaacaggag atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca
    38641 aagtcttata tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa
    38701 gagcgactag ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg
    38761 ctagatcaag caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct
    38821 gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc
    38881 tgcagcaatt gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca
    38941 tgtgatcgtt gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt
    39001 aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa
    39061 caaggtttgc taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta
    39121 caaaaaatca ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc
    39181 ttgtcaagtt caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag
    39241 atgatccatt ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa
    39301 aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga
    39361 aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca
    39421 ccaagttctt gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg
    39481 tgacaaagta acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg
    39541 ctttaaataa ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca
    39601 gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt
    39661 tgcccagcaa atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg
    39721 ctcgtattgc aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata
    39781 tctatggtat tggtaacact actgctaaaa agatcttagc tgatgctggt gtatctgaag
    39841 atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata
    39901 aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg
    39961 aaatcggttc ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca
    40021 cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat
    40081 aattcttaag tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc
    40141 gtgtgaagaa gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta
    40201 tcatcatgat cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag
    40261 gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta
    40321 caaaagcagc aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt
    40381 ctggacgtga agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc
    40441 gtgacgtgac tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat
    40501 gagtgccgct cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg
    40561 aaaggggtaa agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa
    40621 gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta
    40681 gggaattccc tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt
    40741 caaatcgatg gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact
    40801 caaatcatct tgaacattaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc
    40861 cttgaaatcg atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt
    40921 gatgtagaaa ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc
    40981 catgctcgct taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa
    41041 gaagatatgc caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg
    41101 aactatcaag ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg
    41161 gagatttgga cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc
    41221 atgactgaac atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc
    41281 atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta
    41341 gacttgtctg ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa
    41401 cttacaaata aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta
    41461 gaagaagtta aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac
    41521 cttttacgaa ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa
    41581 cgtaaagcga tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact
    41641 actgaagctc gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa
    41701 cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc
    41761 gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat
    41821 attgcacctc gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca
    41881 agacgcggtg atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac
    41941 atcactttat agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct
    42001 agctcagcgc tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt
    42061 ttttttatac cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt
    42121 taagcgagaa ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga
    42181 tgaagaagga gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca
    42241 gccagatgat cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg
    42301 gatcgctatt attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg
    42361 attgcttctg ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa
    42421 tatttggacg atccgacaga tggttggaat gatttttcaa aatccagaca atcagtttgt
    42481 tggttcgaca gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga
    42541 agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt agaatggttg agtttgcttc
    42601 acgcgagcct gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt
    42661 tgccttacga cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg
    42721 tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat
    42781 atctatcact catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca
    42841 aggcgaactt gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat
    42901 cgacttaggt ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg
    42961 ggttgatgtg cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc
    43021 cgttttgaac aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta
    43081 tttgacatca atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga
    43141 agcgggaaat caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca
    43201 gtccatattg gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc
    43261 cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta
    43321 gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc
    43381 ctagcaaaag aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca
    43441 tttgaacttt caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg
    43501 ccggaagtac ttgtattgga tgaaccaacg gcaggacttg atccccaggg cagaaaagaa
    43561 atgatggaga tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg
    43621 catttaatgg atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga
    43681 atcgttaata gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa
    43741 caactaggcg tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac
    43801 tttgctacct tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga
    43861 ggaaatgccc atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat
    43921 ccatcgattg gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt
    43981 tttagcaaat aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt
    44041 cttgtcaaaa gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat
    44101 cctcttcact gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg
    44161 gggtgttttc catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt
    44221 cgtgttgatc atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc
    44281 ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat
    44341 ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa
    44401 gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa
    44461 aatgaaagca gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga
    44521 agatcttgcg actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata
    44581 tcgtgtattg cattggcata atagagacac gatggttatc attgcttttg tattattaac
    44641 tgtgctactg atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt
    44701 gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt agggtctgtg
    44761 gcagactaag gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaattttat
    44821 agaaaaatca ttattccaga taacctttta ggaacaaagg agagaaaaac atggtacgtt
    44881 acaaagcaat cattgcgtac gacggaacaa attttaatgg attccaaaaa cagccaaatg
    44941 ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa
    45001 tcacggtctt tggttcggga aggacagatg ccggagtaca tgcgatcggg caagtaatcc
    45061 attttgatta tccagaggag cgtccgttag aacgaatgcg tttcgcgcta gatacccagt
    45121 caccagagga tattgctgta agacaagtag aaattgtttc agaagacttc cacgcaagat
    45181 atttggtcaa agaaaagacg tatcaatttc gagtggatat agggaaacca cgcagtcctt
    45241 ttcgacgcca ttatgcaagt tattttcctt acccacttga tttggaaaaa attcagcggg
    45301 cgcttcctga tttattaggg actcacgatt tcacctcttt ttgtgcatca ggaagctcga
    45361 tagaggataa aatacgtacg atctacgagg ctaagatgga ggtaaatgaa acaaaagacg
    45421 aattgttgtt tacatttagg ggaaatggct tcttgtataa gatgatacgg atcttagtag
    45481 gaacgctttt gaaaattggc aatggccgat tgcctgaaga tagtatccca gatattattg
    45541 cgaaaaaaga tcgtaatgca gcaggaccta cagcacaccc tgaaggactt tatttgtatg
    45601 aagtgaaata tgaataagag gttatgatag aagtggtcag cttcaagaaa taagaaggta
    45661 tttacgaaaa ttacttctca aaattttgca aaatttcggc ttattttcga agaagctact
    45721 tctggaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaaaga
    45781 gcaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt
    45841 gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt
    45901 gttttgattg cccttggtgc ggacagtctg aacaggttcc atcgtttctt tattttctac
    45961 gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc tggatcaatc gtgccataaa
    46021 aggggcgatt gcggtcaagg taccagacat aaagaataaa caatgcaata cataggacaa
    46081 aaaggattgc acctatcgta aatccttcag gcaagtaact gaaagtgaca gtatgtttac
    46141 ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt gctgaccttg actggtttcc
    46201 catcaatttt agctttccat cctttgtcat aaggaatagt tgtcaccaac atggaatctt
    46261 cagcggtatt tacagtccca gaagcttttc tgccagtggc ttccatttcg acaccttttt
    46321 ctttgatctg attcattgcg gcttgatagg ctacaacatc tagaccaacg actttaggct
    46381 ccatgaaact tatttctttc gtcccataaa aacttgcagt aaatgtcact gttgtctctt
    46441 gaggataata gccaagatca tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg
    46501 taccattgac agtgatcgtc gcactagaac tttctaattg cgcaaaattg gttgggaaca
    46561 aacttaagta agcttgtgta tttgcaggca catggacttc ccaagtgagc tcttttggct
    46621 ggttatctgc tgtttctgag taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca
    46681 actgattctt caaagtaggt gtatagaatg taaaatactg ttcatttaga ccagacaatg
    46741 tattgatcaa gtttgtctga ctggtcaaat tatctgtcac tggctgctca acatcattaa
    46801 cggacaacgg tgcagtaaat gctagtggca atgcattgac attctgatac agtgtatagt
    46861 cgccgctttg accagcttgg cggaagccat atttactgaa cgtgctttcg tttttagaaa
    46921 tgttatattt gattcctgta aacccatcca tcagcaaagt attattgtta taacggatat
    46981 tcaaatttgt ccctcttgaa cggaaaccta atttatccag gtaagaggaa gaattacgat
    47041 ttcttataga agaaaacaaa ctgataccac tgtaaccata gttgatcgaa tcgtttgcag
    47101 atactggatc cagattttcc aagcggtaaa acgagtcgga atcttttttt gtttcattta
    47161 caagagcttt caagtcagga taaggatcag tatataaact gcgggaagca taattccaat
    47221 catcaagaat gccatggatc atactgttcg tattgaccag tgcttcagca gacatgatca
    47281 ataataacaa tatactcaga tgattgaatg aaagtttttt atgctgataa aatcccacag
    47341 caaaagcata aatccctaag aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg
    47401 tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa gagagcagct acgaccaaaa
    47461 tgattcctaa caacagccca ccatcgtctt gttctatctt ttcccatcct tttcctgcaa
    47521 gcaggagcac taagaaagag aacagatagg cataacggaa taaaaacata ttcggtgcat
    47581 gcataccatg ccagaacaaa ttcaatggaa caagataaaa acttgcgata agtatggcga
    47641 aaagactgcc gtacaaaaat ttatttttca acggtacttt ccgactggca aaataaaaca
    47701 gacagaggac taatggtagc agacctgcgt aaacgaacgg aatcgatccg tattttgtcg
    47761 tgtcataaac accaatcata tttttcatga ttagatccag ataagaagtc gcttctgttt
    47821 tcaacgtagt gatctgactc agtgcctcgc catttgaccg aagatccagc actgctggca
    47881 atacaatgat cattgaagcg ccgcctgcca gtaaagaagt gataccataa ggaacaatcg
    47941 tttttttata ttctgaccag tttgtcaata gacgggcgat aaaatataag aaagaaaata
    48001 cgccaatcat aaaacccata taaaaactgg ataaaaacaa gagtaaataa ctgataaata
    48061 aaagtttcgg ccgtttaaga tccataacac gatgaatccc caaaatcact aatggaagat
    48121 aaacaaatgc atccagccac atgatgatct ccgaatgtgc agtgataaat gagatcaatg
    48181 cgtaactgac gctaagtgca acatgccccc agcgaggaat cttgaacgta cgtgcaagaa
    48241 accaaaaagc caatccggca cagccgacct taagcaaagt cagaacatac agtgcatctg
    48301 gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa gatcccgcct agatagtaag
    48361 aaattaaaga taaataattc aatcctagag aagcattcca tgtataaaaa atactttgct
    48421 ttccaagaag aacatttcgg aagctggcat gaaaattaga aaactgtgaa aaggcatcac
    48481 ttgccaaaat acttctgctg cttccaggat agattccgat acttagataa acaagtgcta
    48541 aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg tccgttttct ctaaagaatg
    48601 actttaaatt ttttttcata tttattttcc accaaatttc t
//
LOCUS       DAZAOG010000008        46553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000008 DAZAOG010000000
VERSION     DAZAOG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.008"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHDLD_000390"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHDLD_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN3362153.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHDLD_000391"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHDLD_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN3362154.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHDLD_000392"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHDLD_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN3362155.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACHDLD_000393"
     CDS             1642..2274
                     /locus_tag="ACHDLD_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3362156.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACHDLD_000394"
     CDS             complement(2276..3181)
                     /locus_tag="ACHDLD_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3362157.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACHDLD_000395"
     CDS             complement(3266..3925)
                     /locus_tag="ACHDLD_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3362158.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACHDLD_000396"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACHDLD_000396"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN3362159.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHDLD_000397"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHDLD_000397"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN3362160.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACHDLD_000398"
     CDS             6168..7253
                     /locus_tag="ACHDLD_000398"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN3362161.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACHDLD_000399"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACHDLD_000399"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN3362162.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACHDLD_000400"
     CDS             complement(8563..9366)
                     /locus_tag="ACHDLD_000400"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN3362163.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHDLD_000401"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHDLD_000401"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN3362164.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACHDLD_000402"
     CDS             complement(10244..11251)
                     /locus_tag="ACHDLD_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN3362165.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACHDLD_000403"
     CDS             complement(11258..11902)
                     /locus_tag="ACHDLD_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN3362166.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACHDLD_000404"
     CDS             complement(12002..12760)
                     /locus_tag="ACHDLD_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN3362167.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHDLD_000405"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHDLD_000405"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN3362168.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHDLD_000406"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHDLD_000406"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN3362169.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHDLD_000407"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHDLD_000407"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN3362170.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHDLD_000408"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHDLD_000408"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN3362171.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHDLD_000409"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHDLD_000409"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN3362172.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACHDLD_000410"
     CDS             18158..19027
                     /locus_tag="ACHDLD_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3362173.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACHDLD_000411"
     CDS             complement(19153..20052)
                     /locus_tag="ACHDLD_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362174.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACHDLD_000412"
     CDS             complement(20217..21041)
                     /locus_tag="ACHDLD_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3362175.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACHDLD_000413"
     CDS             complement(21052..22347)
                     /locus_tag="ACHDLD_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN3362176.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACHDLD_000414"
     CDS             complement(22363..22842)
                     /locus_tag="ACHDLD_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN3362177.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACHDLD_000415"
     CDS             complement(22848..23876)
                     /locus_tag="ACHDLD_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN3362178.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACHDLD_000416"
     CDS             complement(23893..24999)
                     /locus_tag="ACHDLD_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3362179.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHDLD_000417"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHDLD_000417"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN3362180.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHDLD_000418"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHDLD_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN3362181.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHDLD_000419"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHDLD_000419"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN3362182.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ACHDLD_000420"
     CDS             29970..30668
                     /locus_tag="ACHDLD_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN3362183.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ACHDLD_000421"
     CDS             complement(30836..33475)
                     /locus_tag="ACHDLD_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3362184.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACHDLD_000422"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACHDLD_000422"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN3362185.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ACHDLD_000423"
     CDS             35318..36493
                     /locus_tag="ACHDLD_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3362186.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ACHDLD_000424"
     CDS             36497..37138
                     /locus_tag="ACHDLD_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362187.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ACHDLD_000425"
     CDS             37135..38052
                     /locus_tag="ACHDLD_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3362188.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ACHDLD_000426"
     CDS             38049..38720
                     /locus_tag="ACHDLD_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362189.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ACHDLD_000427"
     CDS             complement(39261..39749)
                     /locus_tag="ACHDLD_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN3362190.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ACHDLD_000428"
     CDS             complement(39950..40216)
                     /locus_tag="ACHDLD_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3362191.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ACHDLD_000429"
     CDS             complement(40216..40479)
                     /locus_tag="ACHDLD_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3362192.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            41000..41140
                     /locus_tag="ACHDLD_000430"
                     /pseudo
     CDS             41000..41140
                     /locus_tag="ACHDLD_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(41282..43105)
                     /locus_tag="ACHDLD_000431"
     CDS             complement(41282..43105)
                     /locus_tag="ACHDLD_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3362193.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ACHDLD_000432"
     CDS             complement(43703..46408)
                     /locus_tag="ACHDLD_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN3362194.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT        13192 a        10064 c         8521 g        14776 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aat
//
LOCUS       DAZAOG010000009        41252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000009 DAZAOG010000000
VERSION     DAZAOG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.009"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..662)
                     /locus_tag="ACHDLD_000433"
     CDS             complement(192..662)
                     /locus_tag="ACHDLD_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN3362195.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="ACHDLD_000434"
     CDS             992..2632
                     /locus_tag="ACHDLD_000434"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN3362196.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="ACHDLD_000435"
     CDS             2786..4108
                     /locus_tag="ACHDLD_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN3362197.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="ACHDLD_000436"
     CDS             complement(4176..4610)
                     /locus_tag="ACHDLD_000436"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3362198.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5512)
                     /locus_tag="ACHDLD_000437"
                     /pseudo
     CDS             complement(4675..5512)
                     /locus_tag="ACHDLD_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(6254..6880)
                     /locus_tag="ACHDLD_000438"
     CDS             complement(6254..6880)
                     /locus_tag="ACHDLD_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362199.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7175..7510)
                     /locus_tag="ACHDLD_000439"
     CDS             complement(7175..7510)
                     /locus_tag="ACHDLD_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3362200.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7497..7781)
                     /locus_tag="ACHDLD_000440"
     CDS             complement(7497..7781)
                     /locus_tag="ACHDLD_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3362201.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7837..9360)
                     /locus_tag="ACHDLD_000441"
     CDS             complement(7837..9360)
                     /locus_tag="ACHDLD_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3362202.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9353..10528)
                     /locus_tag="ACHDLD_000442"
     CDS             complement(9353..10528)
                     /locus_tag="ACHDLD_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3362203.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10532..10717)
                     /locus_tag="ACHDLD_000443"
     CDS             complement(10532..10717)
                     /locus_tag="ACHDLD_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362204.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10683..12377)
                     /locus_tag="ACHDLD_000444"
     CDS             complement(10683..12377)
                     /locus_tag="ACHDLD_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3362205.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12374..12847)
                     /locus_tag="ACHDLD_000445"
     CDS             complement(12374..12847)
                     /locus_tag="ACHDLD_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN3362206.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12916..13071)
                     /locus_tag="ACHDLD_000446"
     CDS             complement(12916..13071)
                     /locus_tag="ACHDLD_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN3362207.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13205..13585)
                     /locus_tag="ACHDLD_000447"
     CDS             complement(13205..13585)
                     /locus_tag="ACHDLD_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3362208.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13589..13804)
                     /locus_tag="ACHDLD_000448"
     CDS             complement(13589..13804)
                     /locus_tag="ACHDLD_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362209.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13807..14214)
                     /locus_tag="ACHDLD_000449"
     CDS             complement(13807..14214)
                     /locus_tag="ACHDLD_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN3362210.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14480..15955)
                     /locus_tag="ACHDLD_000450"
     CDS             complement(14480..15955)
                     /locus_tag="ACHDLD_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN3362211.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15945..16793)
                     /locus_tag="ACHDLD_000451"
     CDS             complement(15945..16793)
                     /locus_tag="ACHDLD_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN3362212.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16830..17192)
                     /locus_tag="ACHDLD_000452"
     CDS             complement(16830..17192)
                     /locus_tag="ACHDLD_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362213.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17193..17351)
                     /locus_tag="ACHDLD_000453"
     CDS             complement(17193..17351)
                     /locus_tag="ACHDLD_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362214.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17492..18116)
                     /locus_tag="ACHDLD_000454"
                     /pseudo
     CDS             complement(17492..18116)
                     /locus_tag="ACHDLD_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18113..18424)
                     /locus_tag="ACHDLD_000455"
     CDS             complement(18113..18424)
                     /locus_tag="ACHDLD_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362215.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18468..18761)
                     /locus_tag="ACHDLD_000456"
     CDS             complement(18468..18761)
                     /locus_tag="ACHDLD_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362216.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18955..19602
                     /locus_tag="ACHDLD_000457"
     CDS             18955..19602
                     /locus_tag="ACHDLD_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3362217.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19663..20808
                     /locus_tag="ACHDLD_000458"
     CDS             19663..20808
                     /locus_tag="ACHDLD_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3362218.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20889..21194)
                     /locus_tag="ACHDLD_000459"
     CDS             complement(20889..21194)
                     /locus_tag="ACHDLD_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN3362219.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="ACHDLD_000460"
     CDS             complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="ACHDLD_000460"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN3362220.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21647..21913)
                     /locus_tag="ACHDLD_000461"
     CDS             complement(21647..21913)
                     /locus_tag="ACHDLD_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN3362221.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22168..22893)
                     /locus_tag="ACHDLD_000462"
     CDS             complement(22168..22893)
                     /locus_tag="ACHDLD_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN3362222.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23254..23324)
                     /locus_tag="ACHDLD_000463"
     tRNA            complement(23254..23324)
                     /locus_tag="ACHDLD_000463"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23290..23292),aa:Gly,seq:ccc)
     gene            23441..24193
                     /locus_tag="ACHDLD_000464"
     CDS             23441..24193
                     /locus_tag="ACHDLD_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN3362223.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24209..24688
                     /locus_tag="ACHDLD_000465"
     CDS             24209..24688
                     /locus_tag="ACHDLD_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN3362224.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24939..26519)
                     /locus_tag="ACHDLD_000466"
     CDS             complement(24939..26519)
                     /locus_tag="ACHDLD_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN3362225.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26512..27255)
                     /locus_tag="ACHDLD_000467"
     CDS             complement(26512..27255)
                     /locus_tag="ACHDLD_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362226.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27431..28207)
                     /locus_tag="ACHDLD_000468"
     CDS             complement(27431..28207)
                     /locus_tag="ACHDLD_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3362227.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28455..29291)
                     /locus_tag="ACHDLD_000469"
     CDS             complement(28455..29291)
                     /locus_tag="ACHDLD_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN3362228.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29302..30231)
                     /locus_tag="ACHDLD_000470"
     CDS             complement(29302..30231)
                     /locus_tag="ACHDLD_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN3362229.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30423..30776
                     /locus_tag="ACHDLD_000471"
     CDS             30423..30776
                     /locus_tag="ACHDLD_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3362230.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30850..31506)
                     /locus_tag="ACHDLD_000472"
     CDS             complement(30850..31506)
                     /locus_tag="ACHDLD_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN3362231.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31732..33666
                     /locus_tag="ACHDLD_000473"
     CDS             31732..33666
                     /locus_tag="ACHDLD_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3362232.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33752..34975)
                     /locus_tag="ACHDLD_000474"
     CDS             complement(33752..34975)
                     /locus_tag="ACHDLD_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3362233.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34969..36840)
                     /locus_tag="ACHDLD_000475"
     CDS             complement(34969..36840)
                     /locus_tag="ACHDLD_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN3362234.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(36861..37979)
                     /locus_tag="ACHDLD_000476"
     CDS             complement(36861..37979)
                     /locus_tag="ACHDLD_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3362235.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(38062..38895)
                     /locus_tag="ACHDLD_000477"
     CDS             complement(38062..38895)
                     /locus_tag="ACHDLD_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362236.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38888..39805)
                     /locus_tag="ACHDLD_000478"
     CDS             complement(38888..39805)
                     /locus_tag="ACHDLD_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362237.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(39828..40007)
                     /locus_tag="ACHDLD_000479"
     CDS             complement(39828..40007)
                     /locus_tag="ACHDLD_000479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362238.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(40032..41156)
                     /locus_tag="ACHDLD_000480"
     CDS             complement(40032..41156)
                     /locus_tag="ACHDLD_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3362239.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12379 a         8303 c         6865 g        13705 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     5101 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5161 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5221 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5281 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5341 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5401 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5461 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5521 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5581 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5641 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5701 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5761 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
     5881 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
     5941 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
     6001 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
     6061 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa acccatatca
     6121 aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag ttatctatca
     6181 ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa acttcaataa
     6241 gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga gctacataat
     6301 ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt tcccaagata
     6361 acgtaaatag atttgactct ggacttataa agtctacttc accttctagt attactttct
     6421 tatgcaaata ttcctctaat aaatatagac ttgctaaagc atacaacaca ttttccaaat
     6481 ttgcttcctt ataatttgaa gaacgaccat gttttactct gttatatatt ttccaccaat
     6541 ctggtgtatc ttcaggccac ccatctccaa aaggttttag tgaagtcaat gttaaagaat
     6601 tcataaccct aacaccatta tccaaacaca ttaattcgct agatttaata acagctttaa
     6661 aatcagttat gcttttattg ctactgctat caaatccgca aagttctttg aaaataatct
     6721 cacactccga accaatcgct agtagtagtt tttgatattc aatagagaat gttttgaaat
     6781 tattctcatc gagcatcaca aaatctgtgg tattaataaa ctcattttct aaatgtagat
     6841 aatacttcca ataaaaattt gtaaactctg ctttgtccat taaatcacct cttttgaatt
     6901 ataacaaaaa aaacacgtcc acaacagacg tgttctttga tttctacaca atcagcttgc
     6961 caaaaccatc caatcagtcc tagcatgcct gtattaaaat tgaacgttca tcatctatca
     7021 tctttcttag tccagcatgt ttgcaaccac acctacaatt ttcagtccaa caatagattt
     7081 cgactagcta agtcttgcaa aattcaggca gctaaccctt catttactac cttaattata
     7141 ctaaataccc acaacgttta caagtgtaat cattctaaga aatctgttta gctatgatcg
     7201 ttgtataatc tttaaacgcc gttccttcat tgattgaaat aattttataa ctcttgtttt
     7261 tccaagctac tttcatatcg tttgttagtt cataatcttg atcgtatcga ataataaacg
     7321 tcaacgttcc ttcaagaatg gtacctacac tagcaagcac gtcttttagc atttgtgaac
     7381 gcacattagc ccaacattca aatacagttt cttcactatc caccatctgc ccatcttcat
     7441 cttttacccg ttttgtcttt ttaaacttga ctctctcagt caattcattt acattcttaa
     7501 tgattggcat aataagcacc tctcatttgt tgtactaatg cttgtaccgt tactggtatc
     7561 cgttcgctgc tggcttcttg acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc
     7621 aaatcaaatt gctgataatt atccatatct ttatccgttg cgctactatc aatagcactt
     7681 tttatatatt cgcctgctgt atcaatcaat tgttgaataa agtcatcatc aatagtgtga
     7741 tcgatccgca tacttttttt tatgctatcc aaaattacca ttttatctct cctaatgaaa
     7801 aaaaggcagt gggataaacc gccgcccaat atttttctat acttttggtg ttacaactgg
     7861 cgtaaactca atatacactg aagcattttg atcaattttc ttataatcgt tgcgaacgat
     7921 cactgcaagt ccttgtgagt agtaatcgaa tttttcccat tgagtcgtta cttgatttct
     7981 acgagcaaca aagatagatt gtgcaatatc ccccataatc atagggaatg ttccagcttt
     8041 aggattggca aacaatgtat ctgcaatcaa tactactggc ataccgaata atgatttacc
     8101 actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc
     8161 taggtggtaa tatccgcttt ggtttagaat taccatttta tttaatgctg ggtctaatgc
     8221 cacattgtat agttgtttca aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt
     8281 taataaatcc atgatatgct tattgtcagt gttttctacc aatttcgcta actgatcttt
     8341 gacctcttgt acaatattca ctgctgaatc ttccacaacc tcatttgata aggcaatctt
     8401 accagcacga gtttctactt tataatcaac tgaagtaaac atttcagcgt caatatcacc
     8461 aatttcagca agttcagctt ttgttgctaa cactgcttgt tggttagttg ctactggata
     8521 tttaccttgt ccatttgata ctgtttttac tgttgcatat tgagctaagt tataatttga
     8581 acgtttcaaa tcaaagactt caccgattac ttcttcggga acaactgccg ctgcatttac
     8641 agtagtgact ccatcacgca cttcgccttg tgaacggata tattcttcat acccacgcaa
     8701 ttctgttttt tcgttatcga ttaaagtttt ttccatgtta ttactctcct tttgtgcttt
     8761 agaagtggat tctaagctat tgtttgtttg ttttgcttgg ttgttactca taaacgattc
     8821 gtaagaacgc ttatttactt ggacatttga tgaatcataa gcgggaacgg tcacgacact
     8881 aatttcatta agtgctttga ttttatctat tgatcgttca attgtgccat cttctttttt
     8941 gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata gaatcaacaa ccccttttga
     9001 gatattctca tacacatcat ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa
     9061 tcctgtatca tcgacattca atttcaacgt acctgctttg acactagcta gtggctttga
     9121 ataatcgtgt ccatataata agaacacatt tgataaatcc acctctttta atgcttcggg
     9181 tgtaatcact tctacaaagc cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata
     9241 gcctgaaata gttttgccgt ctgttggttg tttttcattt tctttctcta ctttgggaga
     9301 atcggctgtt agctcagctt cttctgtcag ccgtttttcc ttttcttctt cattattcaa
     9361 ctggtgtaac tccttcctgt tcttttaatt gatatttctc caacgtatct aaatacgtaa
     9421 agttcaaact tgctagtaaa cgatcgccac catccatggg gggtagcccc attttcgacc
     9481 gcccctcatt aatcgtgaga agtgaccctt tgacctgatc caatacattt tttacttttg
     9541 tttcaggatc agtctctagt aactgatcgg aattaaagcg gatattggtt tcaagtttcg
     9601 tatctaattc actcacaaac acattaaagt aatgaatcag cgtactttgg atatactgga
     9661 gattactttg aacggtgcta gaatgttcat tttcaacgcc taaacgctca atcggtacac
     9721 caaacgcctt ggcaatttgt tttgtgttcc aatcattgga attgataagc ttcaaaacat
     9781 cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc aagaatgatc gttctaagag
     9841 cattatctcc actacttgaa ccgttggctt cttcaaactt ttcacgaatt gcgttctttg
     9901 ctgatccgtc taaatctgac ttatgaacct tcaaaatacc tgacccactg acaccacgag
     9961 taaagaaatt gtgcaatgtg cggttgccag cttgttgaat tttcagttca tcacgtaacg
    10021 cataaagtgg tggtaatcct gttaaaccat cttgggtgaa atatttgaag tgcaaaatat
    10081 cgctagactt cacacgtctt tttttatcac caatctgata agacaacgta ccattatcta
    10141 attgagtaac cgttacactt gagttaggta ataagtgaat ttcttctacc ttttcaccgt
    10201 tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat attgaccgcc aaagcaaact
    10261 ttaaatgcca accatccatt tctgaattag gtttctcgtt tagtaacttc accagttcat
    10321 tatcagcttg tggcatattg tttttgacca attgaatcgg acttgaagca atatcactag
    10381 caataattcg aacggctgca aacacatcac tatttcttaa tgcacgaacg cttgtaaagc
    10441 tggtggtgta atcatccgat tggattgata ccacatgttg taggaaagga tctcccgttg
    10501 tattttgtct cgctttaaaa aatgccatct tttaacctcc cttctgttct gtatttagca
    10561 tgaccgctac cccaataagc actatgccca gcgctaacaa gccatagtag acatttgtta
    10621 gtaaggtgat agcaaccaac acacacacca atccaagaag taaaagaacg gtatgaatgt
    10681 aattagaaag aaaattcttc acttgtataa aattcattgt ctgctttcgc tccttccgtt
    10741 gtggtgaagt aatccattgc aaacacatag gcattgatta atgcagctat tggatcaatt
    10801 ttgttactgt tctttgattt attaatctga atgccgttgt tatccgtttt taaaatagcg
    10861 ttgttcactg catgggtgag aatcgtattt ttattgtgaa taacgttgcc ttcataaact
    10921 tgttctctaa aagttcgggt cggaacgttt agagtaatcg ttccttgtct tacttctaac
    10981 attgggtagt tggctttttc agctttactg ataagcgaat tagcgttata cgggtcataa
    11041 caaatcccca tacattccaa atcattttct tgaatcaaat cttgaataaa ctgaaatact
    11101 tggtcatagt ccacgattcc actttctaat tgagtaatgg aacactcacc agctttttct
    11161 agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt gaagcccata tttggtggct
    11221 acaaagctat gtgaatcaca ataaagttcg ccgttatcta gcggtacgat ccatgaaacg
    11281 ctagttaaat catctgtttt agataaatcc actccgatat aaacgggctt gcctgtaata
    11341 tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa tcatatagct gtcctcactc
    11401 gcttgtctcc ataaattgaa gtttttcacc aatacagaat taaggttatt ttgtttcaaa
    11461 gcaaggttca catcatcttg aatggttgga atcattactt ttttgatttc ttcactttca
    11521 aaaatcggat tggctttgat ccatttctct tgatcgtgaa tttcttcggg atcgtccaat
    11581 tcccatatcg ctataaagta cctgtcggct tgttcttcgc cttttagcac acgatctaac
    11641 aataagtatt cctcgtacat aggaacgttt aaatcaagcc cagcggtact aataacacac
    11701 aataagccgt tcttttgttg ggtcattcct gacttaatga cgttgtacgt tttacgagtt
    11761 tttgcttcgt gccactcgtc aagaattgcg gttgttggtg caaacccatc tagcgtgctg
    11821 gtttcactag ccagtgccat agcgaacgaa ttagagggca agtgctgaat cttagagttc
    11881 ataattttca gttgtggtct taaaaactta ctagacttga ccacgtttct aagtgaatta
    11941 gacagcatat catagcccaa tttagcttgt ttcaaagcgt tacttacaaa caatacttgc
    12001 cttgcttctg ctggttcttg ttccataatc aacgcattgg cagccatgcc acttgcgata
    12061 tacgttttcc cattcttacg gctcatactg gtaaacgctc gattgaatcg cctgtaattc
    12121 ccttctttag tacgccagcc ataaagactg cctagaatga atttttgaaa tcctaacatc
    12181 ttgattgctt taccgtccgt agatggtagc aactcaatga atttaatcgc tttggtggct
    12241 tgttcttcat cgaacacata agggaagcta tctgacttag aacgctccaa atcgcttata
    12301 tgacgctcac acgcttggat aattttcttg ggtgcaatca gcttacctga taggactttc
    12361 tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt
    12421 taaccttgtt caaagctagt tttgatcgtg attctaaggt catacctagt gaggtggctt
    12481 gttttttcaa atcactcata gcttgtgatt gaacccgaac cgctggattg gctttcttta
    12541 ctccgttttc gcatgtgata aacgtacctg attttcttat ctcggcttcg gctgttttca
    12601 ttcgagcaaa ggctaaacaa taggctacca atgaaccata atctgcttca ctcaatggat
    12661 aatctttttt taatgttggt acgatacgat tccattcact tctagcgctc gctgccatcc
    12721 agtcaggggg cgtgatattt attaatggtt gctgcttgaa caattcttct ctagcgtcca
    12781 cacggattgc tttttcttca cttgatatat tgccttttgt atcgtgtaat attttagggt
    12841 gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac gtaagcaatt aagctcacat
    12901 tccttcgttt ttcgatcaga atttttcttt atctaattta tctatataaa tgatttttcc
    12961 gttctgcatt tgaatggtca cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc
    13021 tgttttatca gtaataacaa ttgtattttt caataaatct cctactttca ttatatactt
    13081 tttcacttgt gattacaagt gtaatcgtta tttgtaatta cccttatgcc ttttcacaac
    13141 ggttatttgt accaaaatga gtaggcatcg attgtcagta agtttcacac tatgccccct
    13201 taactcaaag ggtactcttt tctcgtcttt gtctttcatt ggctgtcttg gaattatggt
    13261 gtgcatggca taaagaacga aaattagttt catctaaccg tctattccaa tcatctttta
    13321 gttcaatttt gtgatctaca acatcagctt tccttatcaa gccttcttct aggcaatcct
    13381 cacatactgg atgatttaac cgatacaagt atgaagcctt tcgccatgtt ctactttgat
    13441 agaattgaaa atatttacct tcgctttgct ttctttcttc acgcttaatt gccgtttgct
    13501 ttgctttatg cttcccacaa tacttctgcc tataatcaat caatgtttta catccagcat
    13561 gattacattg ttttttaact gccatagatt accaaccaaa gcgatctcgt tcgtcagaat
    13621 catcttgttc ccattttgat ttgtattcaa tataaggcga aactatcttc aagcacgttc
    13681 cgtctttaaa attaacttta gtgattttat cttcctcaaa actaataaag tctacatctt
    13741 cttgagtgtt tgaatactca atgtgtttct caccatcgaa gtatctaata ctttccatat
    13801 gcataattat tctacctcca ataaattagt tatcaattca tcatctgtta gtagcaaagc
    13861 caagaactta tcatggatgt ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg
    13921 tctcgctcga tcatataggt taagcagaca attaaattca tccattagct cacctgtatc
    13981 atcatagttg ccactatcta ccaatgatcc cactactagc gattgacacc acgctactga
    14041 ctcctgcagc caatcaggtt cttcttccat tatctttaat acttctggat caaatgtgga
    14101 ataatcttct gttaccaatc ccacactaaa tgccagcgtg ttcattagaa accgatccat
    14161 ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa ataatattga gcatttactt
    14221 tcctccttga ttcgtaacac gtggtaacac ctacgtaaca ggtacccgtt accactcaat
    14281 cccaacgggg acaagggttc tagcgttttg gtaacaggta acgggtaaaa tccagtaaca
    14341 ttattcccca gcatattagc taactatttc aaaagtttta aggaaataag gtgttacccc
    14401 gttaccaaat tgctgtatcc cttggggcag taagggtaaa ctagtaacgg gctaggtgtt
    14461 actgacccgt tactacccgt tactttttgt actttccagc tcgattttta ttttcatagg
    14521 catttgactt attgtaaaat cctcgtcgtt tatttctttt tggcagttgc ttccgttccc
    14581 aaccaggaat actgctaata atcaaggcaa tctttttagt ttctgcatct gatcgaccat
    14641 tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc
    14701 tatcaactaa ataaaactct tgttgcatag tagctccatt atagaaatgc tgtaaatttt
    14761 tctcattaca tttactgtca ggatagttct gataataaga acgtctcgcc cacgctggag
    14821 cttcttccca tttatacggg atttccattt ccaaatattt agtgattgct tctttaattg
    14881 gattctccat cttggcatcc tcttgatagt cttttgcaat ttctaagact tctttatctt
    14941 catcgggact aaaatagatt cgttgtcctt tatcaaaaag cacctttgct tcagctaaca
    15001 cttgaagaaa atattcatcc tttgttttaa acggattcat ctttggctta ttcttacaag
    15061 gcaaaggata aaaacggcgg tttcctgtat catctttcaa atactgccca tcattcgacg
    15121 ttccaataaa tacacattgc cttgcccaag ctatgacatt ccgaccataa ggaggtctaa
    15181 ttttatcgat cttggcactg atgaagtttt ttatctgttc aattttagtt ttgttcattg
    15241 aagataattc tccaagttca ataattacat tgccttgaag ctgcatataa tcatctttct
    15301 tttcacctag cgaatctaat gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa
    15361 cggtactctt accaatccct tgcggtccat ccaaaactgg aacgatctca aactttattc
    15421 ctggtttata gatacgtgcg actgatccga ctatccaacg ttttgttact tctcgtgtgt
    15481 aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat
    15541 cccatttaac tttttcgata cgttctttta ctgggttgaa aacattcttt ctagccacat
    15601 ttactactgc tttgtgtatt ttgttgtcat caaaaactac attgtagttt tcttcaatat
    15661 atgccgttaa ggcatcctca aaactgtctt cccattctcc agcggtacga ttgatccaag
    15721 gagaatcttt taataggtga atgtatccgc taaattcgtt ataagcaatt gttccagcta
    15781 aattgctatc attggttaaa atcaattctg cattcacaac actgttactt ttaatctgat
    15841 cgtctttatt gcatcttaat cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac
    15901 gctcgctggc ttcgggaaag tctaaaatac tatctaattt atgcttatga gccatgagct
    15961 ttccccctca aatctttttt aatcatcgat cgaataattt gattcacttc tttatctttt
    16021 agcggtggtt cattgtacgc atttgctgta taaatcaatt ctgctaaagt ttgattatct
    16081 acaccgtgcc atagtaacca accgcataac ttagttaaat gatcgtttct actaccttcg
    16141 ccagcgcctt gaacaatcat atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg
    16201 tattgagtct ttatttcttg cggttttttt agcagtaggt tcactagcca atcgggagct
    16261 ggtgcaatat tatttagaga tacgttttca ataggttcat atgctctccc aacacttctt
    16321 gaaataatca tagttggcgc tattggaact cctttagtga taatatcaat cccgcttaaa
    16381 cttttacctt tatcatcacg cccgaagata tcagcaaatg cacctgtttc ttgttggata
    16441 attacaccat caggaatttt aaagaagaaa tggattcctt tacctggtgt gatttccgta
    16501 taagtagaag gaaatggatc atacattgaa gcaagttttt tataattctc aacgccgttt
    16561 cgtgtttcag aatgttgatc taggtcaact accaccattt gatgatcttc aagcattaac
    16621 ccaatatttc tagttgcatc ttccgtccac cattcattaa tttgattaga atcgttagtt
    16681 gcatcattcc aaccactaga gccttttatt ggaattttag ttccaggaga taagggataa
    16741 attgaccatc ccgacttttg taattccgtt gctgcttgat aattatttgc caataaacct
    16801 ctcctccttt ttcaaaatac tggtataaat cattcagaca cctccatgag cttcaactgc
    16861 tcaagttcgt atttcaaccc atcccgaaca aattcagcac cttttaattg attctgatac
    16921 tttacccatg tattaaatgt tttcaccctt gcttcttgca tatcagtaat gtttaaccat
    16981 gcggttaaaa tctgattttt tagctcgttg atttctcgac aaattatttt gtgttctgca
    17041 tgttttttga gtacatacaa aactgttagt ttttcattga atgaatttcc aaagtaggtt
    17101 ttcccgatat tattttctag ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta
    17161 tcgagttggt ttaatagttt tgttttgtac atctacttat gccctccagt tcttaattga
    17221 tagtcgtact cgtcataagc agtgaagaga tataacgcta atcctgcaaa gatagctatt
    17281 tttgcaatta atggtaataa gccgaaaatt ccgccgatta aaatgcttcc tataattaag
    17341 tttcgtttca taatggttcc ctccgatttt ttaatatggt ataatcggaa caaagaatat
    17401 cgggttttat cccaattaag ttgcctttat cctgtccgcc aagactgtta tggataaggc
    17461 tttttctttg ttctcgatca tgttaatgct cctaagttgt ctgcaattca gcagaacgat
    17521 tataaatttt ctcactcact gaaaactgca acccatattt ttctttaaca gtcattaatt
    17581 ctactgtctc atctaaaata ggttcacgat caaccaacat ttcaggagtc atatctttct
    17641 tttttaccat tttggaatga ccataaatag ttgatacagc tttattggct atcacattcg
    17701 ctttaatgta gtctactggt tttggttcag taatagcgtg gcttaattta gtcattgctt
    17761 ctttttggtg ttctttgtct agcatgcgga atacttgaaa gccttcaagt cctgtggact
    17821 ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac
    17881 ggcttttgaa tgccatgcgg taaatatttt ttttcgttga tgatgtttac atcttgtgtt
    17941 ctgcccaatg aatcgatgac cttactagta gtaaccccat ctttaggcaa agagtgaatg
    18001 gctctagtaa cctgtttaag acctaacgct tccgcaacat ctttcgccac tgcccaccat
    18061 tcatcattaa tatcgacaaa tcttataatg tgtccgttcc aaatttcagt tttcattcaa
    18121 cgtcctccag ttcaagccct agcaagctcg ctaacaactc aacttgctga tacaaaattt
    18181 cttgatactc ttcaatagaa agtcgttctt taaactctgc ttgtactaaa tcgttcaagc
    18241 cacgcaatac tccgcttacc gttggttctt gtggtagcaa tttttgatag tccatttgtt
    18301 cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc caagtttgct aattctgccg
    18361 ctgtgtcttc tgtaagaatt gttgataata ctttttcgat tgctttattt tgtgtcgatt
    18421 ccattattat ttcctcctat taatcccttt gcccccacgg cttgcgttta tttttggtat
    18481 tcttccataa agcgatcaca ttcatcttgt gaaattctta cgactccatc aactataatc
    18541 aactttaaac cgttaggaac atatttctgc atcatagtat tgtatgaaac gttcatatac
    18601 ttagcagctt gtgccttgtt catatatttt ggaaactggt actttttttc taataatgaa
    18661 agtaacgttc cttgagtttg ctctacttgt tgaacaactc gatcgtcaat aattttaaaa
    18721 gctggatcat caagcgtgag tttgtcaatt tttaattcca tatttatccc ccctcgttag
    18781 catacatttc tatacaaaga ttttaaaaaa atgcaagagg ctcttgaata catttctata
    18841 cctcttataa actcaatata ccatcatacc ttttcatttg caagaatttt aacatacttt
    18901 tttgaacttt tatgtttcac acatatttaa atcgtgatat agtagagaaa atatatgaat
    18961 ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag tggagacagg acaaagaatt
    19021 agactggcta gaattgaaaa gggactctca atggaacaac ttggaggtaa attatcccca
    19081 ccggccagta aaggtgccgt tagtaattgg gaaaatggct ataatcttcc caataacacc
    19141 aggcttaaac aactttcaaa ggtattagat gtatccacta cctatctatt aaacggatct
    19201 tatacattaa gagatatgca tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa
    19261 ttgaaaaaaa ctattgaaaa aaataactct ataattgatt ctaatctttc taatgctatc
    19321 aataacatcg aagaagataa cctgactgtc tcacagaaga tttatttaac aaacgcaata
    19381 aaatatctta aaacaggatc tcgaattgaa tttcctgacg ggagtacccc aattgcttat
    19441 ctcgcagctc ttctaaatac gcttttacga tatgactcat taattaatga ttcagaaaaa
    19501 aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa atgatcttct aaaagagttc
    19561 acagctcttg ttactgagct aaataaacac tattcaaaat aataataaac tgcctcctcc
    19621 aaccgccccc acggcttgct acactatgga gggaaaacta ttatggcaac attcaagcaa
    19681 tatgaaacta aaaaaggtaa attttggctc tatgaagcct atcttggaat gaacaaaatg
    19741 actggcaaac ctgataaggt tcgtagacga ggttacaaaa caaaaaaaga agcccagctt
    19801 gccttgtcac gcttgcaagt ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa
    19861 acgtttaaag aagtttatga cttgtggcta gaaacatata aaaccactgt ccgagaagcc
    19921 accttcatga aaactgaaat caagtttaga aagtggattc ttccaaaata tggaaatctt
    19981 cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg taaatagctg ggctgaaact
    20041 actgaccaat acaaggtact tcattcaact tctaagcgta tttttagcta cgctatcaat
    20101 cttgggatta ttgagactaa tccactagac aacgtattaa tgcctaaaag aaccaccact
    20161 aagcataaag aaaaggtcaa agtttacagt aaagaacaac tgatcacctt gtttgattac
    20221 ctaaacagca aacctgaaac ttatcgaaac gactttgata aaacgttact acgctttctc
    20281 ttttatagcg gggttaggat cagtgaagcc cttgcattga attggtcaga cattgatttt
    20341 caagaaaata cggtcaccat caataaaaca ctaagccaat ccaaaaatgg ctataaaatc
    20401 agtgatccta aaactgattg tagtgctggg gttcttccac tagatgaaag aacaatttct
    20461 atattaaaaa aatggcagat taatcagcgg aaatatatgc tgacacttgg tgtaactgat
    20521 ccgactatga ttttttgtgg tatctataaa gaactaatca cacaccacgc tatttatgca
    20581 agattgatta ctatttgtga aaaagctatg gttccatttc tcggtgtgca tgtagcaaga
    20641 cacactcatg cttcaatgct cttggacgct ggagctacta tggtagaagt tcaaactaga
    20701 ttacggcata caaaaattag tcagacgatc gattgttacg gtcacttggc aaaagaaacg
    20761 aaagaaaaga ctgttgaaaa attagtacaa cacttaaata ttgactagat ggcatacaat
    20821 atggcataca gtaaaaaata aaaaaaggag aaaccctatc atatcaaggt ttctcctagc
    20881 tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    20941 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    21001 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    21061 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    21121 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    21181 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    21241 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    21301 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    21361 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    21421 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    21481 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    21541 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    21601 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    21661 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    21721 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    21781 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    21841 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    21901 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    21961 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    22021 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    22081 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    22141 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    22201 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    22261 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    22321 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    22381 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    22441 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    22501 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    22561 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    22621 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    22681 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    22741 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    22801 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    22861 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    22921 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    22981 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    23041 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    23101 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    23161 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    23221 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    23281 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    23341 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    23401 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    23461 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    23521 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    23581 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    23641 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    23701 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    23761 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    23821 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    23881 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    23941 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    24001 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    24061 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    24121 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    24181 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    24241 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    24301 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    24361 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    24421 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    24481 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    24541 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    24601 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    24661 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    24721 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    24781 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    24841 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    24901 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    24961 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    25021 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    25081 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    25141 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    25201 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    25261 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    25321 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    25381 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    25441 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    25501 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    25561 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    25621 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    25681 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    25741 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    25801 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    25861 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    25921 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    25981 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    26041 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    26101 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    26161 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    26221 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    26281 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    26341 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    26401 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    26461 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    26521 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    26581 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    26641 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    26701 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    26761 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    26821 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    26881 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    26941 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    27001 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    27061 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    27121 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    27181 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    27241 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    27301 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    27361 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    27421 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    27481 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    27541 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    27601 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    27661 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    27721 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    27781 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    27841 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    27901 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    27961 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    28021 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    28081 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    28141 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    28201 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    28261 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    28321 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    28381 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    28441 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    28501 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    28561 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    28621 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    28681 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    28741 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    28801 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    28861 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    28921 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    28981 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    29041 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    29101 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    29161 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    29221 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    29281 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    29341 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    29401 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    29461 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    29521 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    29581 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    29641 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    29701 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    29761 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    29821 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    29881 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    29941 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    30001 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    30061 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
    30121 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    30181 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    30241 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    30301 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    30361 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    30421 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    30481 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    30541 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    30601 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    30661 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    30721 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    30781 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    30841 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    30901 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    30961 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    31021 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    31081 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    31141 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    31201 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    31261 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    31321 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    31381 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    31441 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    31501 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    31561 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    31621 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    31681 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    31741 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    31801 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    31861 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    31921 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    31981 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    32041 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    32101 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    32161 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    32221 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    32281 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    32341 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    32401 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    32461 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    32521 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    32581 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    32641 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    32701 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    32761 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    32821 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    32881 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    32941 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    33001 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    33061 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    33121 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    33181 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    33241 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    33301 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    33361 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    33421 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    33481 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    33541 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    33601 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    33661 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    33721 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    33781 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    33841 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    33901 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    33961 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    34021 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    34081 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    34141 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    34201 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    34261 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    34321 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    34381 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    34441 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    34501 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    34561 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    34621 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    34681 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    34741 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    34801 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    34861 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    34921 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    34981 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    35041 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    35101 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    35161 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    35221 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    35281 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    35341 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    35401 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    35461 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    35521 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    35581 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    35641 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    35701 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    35761 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    35821 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    35881 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    35941 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    36001 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    36061 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    36121 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    36181 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    36241 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    36301 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    36361 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    36421 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    36481 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    36541 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    36601 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    36661 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    36721 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    36781 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    36841 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    36901 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    36961 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    37021 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    37081 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    37141 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    37201 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    37261 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    37321 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    37381 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    37441 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    37501 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    37561 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    37621 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    37681 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    37741 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    37801 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    37861 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    37921 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    37981 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    38041 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    38101 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    38161 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    38221 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    38281 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    38341 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    38401 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    38461 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    38521 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    38581 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    38641 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    38701 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    38761 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    38821 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    38881 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    38941 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    39001 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    39061 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    39121 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    39181 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    39241 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    39301 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    39361 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    39421 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    39481 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    39541 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    39601 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    39661 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    39721 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    39781 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    39841 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    39901 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    39961 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    40021 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    40081 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    40141 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    40201 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    40261 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    40321 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    40381 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    40441 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    40501 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    40561 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    40621 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    40681 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    40741 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    40801 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    40861 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    40921 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    40981 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    41041 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    41101 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    41161 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    41221 ttagtaacac aaatatttat ataaaaaaat at
//
LOCUS       DAZAOG010000010        39824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000010 DAZAOG010000000
VERSION     DAZAOG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.010"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..921
                     /locus_tag="ACHDLD_000481"
     CDS             52..921
                     /locus_tag="ACHDLD_000481"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN3362240.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1086..2357
                     /locus_tag="ACHDLD_000482"
     CDS             1086..2357
                     /locus_tag="ACHDLD_000482"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN3362241.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2354..3649
                     /gene="rho"
                     /locus_tag="ACHDLD_000483"
     CDS             2354..3649
                     /gene="rho"
                     /locus_tag="ACHDLD_000483"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN3362242.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3748..4011
                     /locus_tag="ACHDLD_000484"
     CDS             3748..4011
                     /locus_tag="ACHDLD_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN3362243.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4456..4905
                     /locus_tag="ACHDLD_000485"
     CDS             4456..4905
                     /locus_tag="ACHDLD_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3362244.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4962..6380
                     /locus_tag="ACHDLD_000486"
     CDS             4962..6380
                     /locus_tag="ACHDLD_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3362245.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6380..7240
                     /locus_tag="ACHDLD_000487"
     CDS             6380..7240
                     /locus_tag="ACHDLD_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN3362246.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7414..8187
                     /locus_tag="ACHDLD_000488"
     CDS             7414..8187
                     /locus_tag="ACHDLD_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3362247.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8334..8699
                     /locus_tag="ACHDLD_000489"
     CDS             8334..8699
                     /locus_tag="ACHDLD_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN3362248.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8847..10055)
                     /locus_tag="ACHDLD_000490"
     CDS             complement(8847..10055)
                     /locus_tag="ACHDLD_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3362249.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10074..10352)
                     /locus_tag="ACHDLD_000491"
     CDS             complement(10074..10352)
                     /locus_tag="ACHDLD_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362250.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10545..11201
                     /locus_tag="ACHDLD_000492"
     CDS             10545..11201
                     /locus_tag="ACHDLD_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3362251.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11643..12440)
                     /locus_tag="ACHDLD_000493"
     CDS             complement(11643..12440)
                     /locus_tag="ACHDLD_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN3362252.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12437..13621)
                     /locus_tag="ACHDLD_000494"
     CDS             complement(12437..13621)
                     /locus_tag="ACHDLD_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN3362253.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13646..14893)
                     /locus_tag="ACHDLD_000495"
     CDS             complement(13646..14893)
                     /locus_tag="ACHDLD_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN3362254.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15108..16853
                     /gene="dnaX"
                     /locus_tag="ACHDLD_000496"
     CDS             15108..16853
                     /gene="dnaX"
                     /locus_tag="ACHDLD_000496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN3362255.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16873..17187
                     /locus_tag="ACHDLD_000497"
     CDS             16873..17187
                     /locus_tag="ACHDLD_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN3362256.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17212..17808
                     /gene="recR"
                     /locus_tag="ACHDLD_000498"
     CDS             17212..17808
                     /gene="recR"
                     /locus_tag="ACHDLD_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN3362257.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17872..18516
                     /gene="tmk"
                     /locus_tag="ACHDLD_000499"
     CDS             17872..18516
                     /gene="tmk"
                     /locus_tag="ACHDLD_000499"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN3362258.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18531..18860
                     /locus_tag="ACHDLD_000500"
     CDS             18531..18860
                     /locus_tag="ACHDLD_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN3362259.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18874..19812
                     /gene="holB"
                     /locus_tag="ACHDLD_000501"
     CDS             18874..19812
                     /gene="holB"
                     /locus_tag="ACHDLD_000501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN3362260.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19848..20672
                     /locus_tag="ACHDLD_000502"
     CDS             19848..20672
                     /locus_tag="ACHDLD_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN3362261.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20665..21012
                     /locus_tag="ACHDLD_000503"
     CDS             20665..21012
                     /locus_tag="ACHDLD_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN3362262.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21081..21953
                     /gene="rsmI"
                     /locus_tag="ACHDLD_000504"
     CDS             21081..21953
                     /gene="rsmI"
                     /locus_tag="ACHDLD_000504"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN3362263.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22062..23183
                     /gene="dinB"
                     /locus_tag="ACHDLD_000505"
     CDS             22062..23183
                     /gene="dinB"
                     /locus_tag="ACHDLD_000505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN3362264.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23237..23839)
                     /gene="nrdG"
                     /locus_tag="ACHDLD_000506"
     CDS             complement(23237..23839)
                     /gene="nrdG"
                     /locus_tag="ACHDLD_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN3362265.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24154..26343)
                     /gene="nrdD"
                     /locus_tag="ACHDLD_000507"
     CDS             complement(24154..26343)
                     /gene="nrdD"
                     /locus_tag="ACHDLD_000507"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN3362266.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26669..27436
                     /locus_tag="ACHDLD_000508"
     CDS             26669..27436
                     /locus_tag="ACHDLD_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362267.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27426..29468
                     /locus_tag="ACHDLD_000509"
     CDS             27426..29468
                     /locus_tag="ACHDLD_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362268.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29749..29898
                     /locus_tag="ACHDLD_000510"
     CDS             29749..29898
                     /locus_tag="ACHDLD_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN3362269.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29909..31423
                     /gene="dltA"
                     /locus_tag="ACHDLD_000511"
     CDS             29909..31423
                     /gene="dltA"
                     /locus_tag="ACHDLD_000511"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN3362270.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31420..32625
                     /gene="dltB"
                     /locus_tag="ACHDLD_000512"
     CDS             31420..32625
                     /gene="dltB"
                     /locus_tag="ACHDLD_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN3362271.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32670..32903
                     /gene="dltC"
                     /locus_tag="ACHDLD_000513"
     CDS             32670..32903
                     /gene="dltC"
                     /locus_tag="ACHDLD_000513"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN3362272.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            32906..34174
                     /gene="dltD"
                     /locus_tag="ACHDLD_000514"
     CDS             32906..34174
                     /gene="dltD"
                     /locus_tag="ACHDLD_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN3362273.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34315..35397
                     /locus_tag="ACHDLD_000515"
     CDS             34315..35397
                     /locus_tag="ACHDLD_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN3362274.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35397..36200
                     /locus_tag="ACHDLD_000516"
     CDS             35397..36200
                     /locus_tag="ACHDLD_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN3362275.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36270..37010
                     /locus_tag="ACHDLD_000517"
     CDS             36270..37010
                     /locus_tag="ACHDLD_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3362276.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37021..38025
                     /locus_tag="ACHDLD_000518"
     CDS             37021..38025
                     /locus_tag="ACHDLD_000518"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN3362277.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38070..>39824)
                     /locus_tag="ACHDLD_000519"
     CDS             complement(38070..>39824)
                     /locus_tag="ACHDLD_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362278.1"
                     /translation="LTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYT
                     RTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPG
                     LSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTM
                     IIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVH
                     SFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPEL
                     MPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQT
                     TINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQE
                     VNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILAR
                     YQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLS
                     IHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKD
                     TFIDRITVQLSRNYPNPV"
BASE COUNT        12770 a         7015 c         8267 g        11772 t
ORIGIN      
        1 aagaattgtc ttaacaaatt tagttttaca aaacttagga ggaattttat tatgccagta
       61 gtatcaggag ctgaattttt aaaagctgct cgtaaaggcg gttacgctgt cggcggattc
      121 aacacaaaca acttggaatg gactcaagca atccttgaag cggctgaagc aaaacaagca
      181 ccagtactta tccaaacatc aatgggtgca gctaaataca tgggtggtta caaaatctgt
      241 aaagatttaa tcactgattt agttgaagca atgaacatca ctgttccagt tgctatccac
      301 ttagaccatg gtgattacga agctgctttg gaatgtatcg aattaggcta tacttcagta
      361 atgttcgatg gttcacatct accatttgaa gaaaacttga aattagctaa agacgttgtt
      421 gaaaaagcac atgcaaaagg tatctcagtt gaatgtgaag ttggttcaat cggcggagaa
      481 gaagacggaa tcatcggtaa cggcgaatta gctgatattg aagaatgcaa acaaatggtt
      541 gctacaggta tcgactactt agcttgcggt atcggtaaca tccatggtca atatccagaa
      601 aactggaaag gattagcttt tgaccactta caagcaattg ctgatgctgt aggtccagac
      661 atgcctttag tattacatgg cggatcaggt attcctcaag atcaaatcga aaaagctatc
      721 tcaatgggta tctcaaaagt aaatgttaac actgaattcc aattatcatt tgctgcagct
      781 actcgtaaat atatcgaagc tggtaaagat aaagaaggta aaggattcga ccctcgtaaa
      841 ttattagctc caggtaaagc agcaatcgtt gaagatgcta aatctcatat cgactggttc
      901 ggttcagcta acaaagcgta agcatccaca taacagatac gatatatagg aatggagaac
      961 gacttttgaa gtcgctctcc attctttttt acttaaaacg gcagaccatg ctcgtttctt
     1021 ttttttatct atgataaaat acaagcatat ccatgaaaaa agattaataa acaaggtgga
     1081 aacagatgaa aaaaatcgta atcaaaggaa accgaccttt agctggtgaa gtaacgatca
     1141 gcggtgcaaa aaatagtgca gtcgcattga ttccagctgc aatcttagct gattccccag
     1201 tgatcttgga aggtgtgccg gacatacaag atgttcattc attaattgaa attttagaga
     1261 tcatgggcgc aactgtccat ttttccaaca acatactaga aatcgatcct cgagaagtcg
     1321 tatcgattcc gatgccgcac ggaaaaatca atagtttgcg tgcttcttat tattttatgg
     1381 gaacactgct tggtaaattt ggcgaagcgg tagtaggcct gccaggtggc tgttatttag
     1441 gccctagacc gattgaccta cacgtcaaag gatttgaagc tctaggtgct gaagtgacaa
     1501 atgaacatgg tgccatgtat ttacggacag aaaataaagg attgcgtggc aatcggattt
     1561 tcatggacgt ggtttcagtc ggagcaacga tcaatgtgat gttagctgct gtaaaagcaa
     1621 aaggacagac agtaattgaa aacgctgcac gagaaccgga aatcatagat gtggcaactt
     1681 tactgaataa tatgggggca aaagttcgag gagcgggtac agatgtgatc cgtatcgaag
     1741 gagtcgaaac attacatggc tgtcggcatt tcatgattcc tgaccgcatc gaagcaggaa
     1801 cctatctagc attagcagct gcagtaggaa atggtatcaa agtgaaaaat gtgatctttg
     1861 aacatttgga aagctttata gcaaaattgc aggaaattgg tgtccatatg aaaatcagcg
     1921 aagatgaaat cgaagtttac ccctcgaaag aattaaaacc cgcaaacatc atgacctatc
     1981 cttatccagg atttgccaca gatctgcagc agccacttac tgcgttacta ttgatgacta
     2041 caggcacttc agaaatcatc gatacgatct atgcgaaacg tgtgaaccat gtgccggaac
     2101 tagcacgaat gggcgctgat attaccgtgg aaggaaatat gatcatcgtg aatggtccaa
     2161 ataaacttca cggcacagaa gtcgtggctt ctgatcttcg ggcaggtgct tgtttagtga
     2221 ttgctggact attggcagaa gggacaacga cgatttacaa tgtagattat atcttacgcg
     2281 gttatgatca tattatcgag aaactaacag ccttaggggc atcaattgaa atgatagagg
     2341 aagtgggagc tgaatgagcg attatttgac aatggcagaa ctagagaaca aaacgttaaa
     2401 agaagtttat agttatgcca aagaatttaa aattccttat tacagtaaga tgaataaaaa
     2461 ggaattatct ctagctgtga ttcgtgcaca agcagagaaa caaggatttt attatatgga
     2521 aggaatattg gatatcgttg gtcaagatgg ttacggtttt ttacggccaa tcaattatgg
     2581 accaagtgca gaagatatct atatttctgc ctcacaaatc agacgattca gtttgagaaa
     2641 tggggacaaa gtggcaggaa aagcccgtcc acctaaagaa tcggaacgtt attatggttt
     2701 gatgcacgtg gaaagcgtta atggaaaaga tccggaagaa gcaaaagaac gtccgcattt
     2761 tccagcctta acacctcttt atccgcaaag acagcttcaa ctggaaacaa caccgcaaag
     2821 actatctacc cgaatgatcg atatcttttc tccaatcggt tttggtcagc gtggattgat
     2881 cgtcgcaccg ccaaaagcag ggaaaacttc tgtattaaaa gaaatcgcaa atggtatcac
     2941 cgaaaactat ccggaagtag aattaatcgt tctactgatt gatgaacgac cggaagaagt
     3001 aacggatcta gaacgaagcg tgaaaggcga cgtggtatct tctacatttg atctgcagcc
     3061 gcaaaaccat actcgagttt ctgaattagt attggaacgc gcaatgcgtt tagtcgaaga
     3121 taaacgagat gtcgttatct tgatggatag tattactaga cttgctcggg cctataactt
     3181 agtcgttcca ccaagcggac gtacattgag tggcgggatt gacccagccg cattgtataa
     3241 acctaagaaa tttttcgggg cagcaagaaa catcgaagaa ggtggcagtt tgacgatttt
     3301 agcgaccgct ttagtcgata caggaagccg tatggatgac gtgatctatg aagaattcaa
     3361 aggaactgga aatctagaat tacaactttc acgtgaatta tctgaacgtc ggatcttccc
     3421 agccatcgat atcaaaaaat caggtacccg aaaagaagaa ttattgatgc caagtgaaga
     3481 attagaagaa atttggaaac ttcgaaaaaa tatgactgga gattcattag aatatacgga
     3541 acaactgatc cgctttttgc gtaaaacaaa aaacaatcag caatttttca aagaatttca
     3601 agatgtctcc tttggtaaaa aaaatccgac cagaaatctg aaaagataat tgcaaccttg
     3661 actgaaacat gttaagatgt taatgttgta aatcgacaaa tcaactctgg ttcagcaaat
     3721 ggagcagggc aaaggagcga aaaaatcatg aaagaaaaca tccatccaga ttaccaccca
     3781 gtagtattca tggattcaac aactggtttc aaattcttgt caggttcaac aaaaacttca
     3841 caagaaacag ttgaatggga agacggtaat acatacccag tcatccgtgt cgaagtaact
     3901 tctgactcac atccattcta cactggacgt caaaaattca cacaagcaga cggacgtgtg
     3961 gaccgtttca acaaaaaata cggtctcaaa gacgcaaacg ctgcggaata ataaaaacgc
     4021 tgataactca acgtttagca gactatctga tagggatagt ctgttttttt atgttttttg
     4081 ccaatttaaa acttcgtttg atgctgaaaa agtcgtagga tcataatgca caaaacaatg
     4141 aaaaactagt atatagtttt aattatccac ttgataaaaa caccttatat taacgaaatg
     4201 aagaggggtt atttcgaagt aaaaaaccat ttacctaaaa ttagaagcta agctttcctt
     4261 tcaaggaagg ctttttgttt tgttatagac ttgcgattgt ttgtgagcta gatcgttcat
     4321 tttaatgact tatacacgcg gatgatgggt ctttttcctc tgttcattca taatattgaa
     4381 cggtacagct agcgatgcta tactgacctc agttccaaga aaacgttaac attttttgtg
     4441 ggggagaggt tttttatgag gtttgatcaa ttgatcgcag aagaactgat tgttttcgat
     4501 ccatcgatag attcaaaagg atcattattt gagcgaatag gggatgtgtt gaaaaaaaca
     4561 aataaggtaa gaaatgttca aaaagtcgtt aaggcactat tcaaacgaga ggaggagatc
     4621 tccacaggaa tagaagatgg atttgggatt ccccatgcaa aaagcaaata tgtgacaaaa
     4681 ccagcactgt gtttttttca tacagccaaa ttaacggact atagcgggat agatgaaatg
     4741 ccaattgaat atatttttgc catcgtagtt cctgaaaaat cagcagatat gcatctagag
     4801 attctcagca cactctccag acgattgatg aatgcagatt ttagaaaggg aataaaagaa
     4861 gccactacgc cagcagatat catcagacag attagtcaag aatgaaaata gtagagtctt
     4921 tgtccgagta tcgatagtga agaaaaagga ggaaaataat catgaaaatc gttggtgtaa
     4981 ctgcttgccc gactggaatt gctcatactt atatgtcggc agaaaaatta gaagtaacag
     5041 caagaaatct aaaccatcaa gcaaaattcg aaacacaagg tgtaaaaaca gaaaatcagt
     5101 tatctgaaca agaaatcaaa gaggcagatg caatcattct tgcagtagat aaagaaatcg
     5161 aattggaccg atttgccggg aaaaaagtaa agcgtgtctc tacaagccga gcaatcaaag
     5221 aaccgcaagt agtaatagac gaagcattga gagatattgg cgtattcgtt gtgtctaatg
     5281 aaaaagaacc agctgcaaat gagaaaaagc cgacaatcta taatcatttc atgaatggtg
     5341 tgaattacat gcttccattt gtgatcgcag gcggaatcat catcgcactg agttttgcct
     5401 ttgggattac agcggctgat cccaactcag cagactataa tgtgttagca gctgcatttt
     5461 caaggatcgg aggagataca gcgtttgcca tgatggttcc tgcgttaggc gcggggattg
     5521 ctacatcgat tgctggaaaa gcaggttttg ccccaggaat tgttgccgga ttactggcct
     5581 ctacaggtgg ttcaggtttt cttggcggaa tgattggtgg cgtgctagca gggtatatct
     5641 gtgattttct ggcaaataaa attagcgtaa aaaaagaatt ttctgctatt tatcagctga
     5701 ttgtcgtccc ctttttatcg atcttaatta ttgggctttt gatggtattt gtgattgaac
     5761 aaccgatcac atgggtgttg gatgctttga caaattggct gaatagcttg ggaaatacat
     5821 ccgggctatt gttcggatta cttatcggga tcatgatggc agctgatatg ggtgggccga
     5881 tcaataaatc gacttcgact ttctcgatcg gtttgatgtc agctggagta aatgcaccga
     5941 ttgctgcttg tatggctgca ggaatgacac ctcccctagg tattgcttta gccactttat
     6001 tattcaaaca taaatttacc aaagaagaac ggacttcagg tcaatcatgc tggattttag
     6061 gcgcttcgta tattacagaa ggtgctatac cgtttgcggt atctgacccg atccgcgtga
     6121 tacctagttt gatgacaggt tcagcagtcg ctgcagggat ttccatggca gtaggagtta
     6181 cttcaatggc acctcatgga gggatttggg tgatgctaat tccaaatgtt atgaacaatt
     6241 tacctatgta tgtcttggca attgcatcag gtacagtcac tacagccttt atgcttggac
     6301 tattgaaaaa aacagtgaaa gagaaaaaac aagacaagtg gacgaaaaat gaaaatctaa
     6361 caaatgaggt ggaagcgtaa tgttatatac aatgaaagag ttattgaaag tggctaaaga
     6421 gaatcaattt gcagtaccag ccttcaatat ttgcagctat gatatgatga aagcaatcat
     6481 cgaagaagca gagagattag aagcaccagt aatcttagaa attcatcctg atgaaatcgc
     6541 ctatttaagt gatgagtttg ttgcggcagt aagagcctat gcttatacaa gtaaactgcc
     6601 gatcgttatc cacttggatc atggcggtac gatagaagat gtcttacggg cgattcgaaa
     6661 cggctattct tccgtgatga tcgatgcgtc tttgaaatca ttcgacgaca atgtacagat
     6721 tacgaaaaaa gtggttgcgt tagctcatgc ggtaaatgtc tcagtggagg ctgagcttgg
     6781 aacgattggg aataatggtt ctgctgaagg aggtgctgaa acaatcattt atacagatcc
     6841 tgatcaagcg aagatttttg tgaaagaaac aggaatcgat acacttgctg tagcaattgg
     6901 aacagcgcat ggcttgtatc cgaaaaacag aactccaaaa ttaaatatcg acttactgaa
     6961 agaattgaac caacaaatcg atcttccatt tgttttacat ggcggttcag gtaatccgga
     7021 cgcagaagtc agcgaagcag taaaatacgg agttgccaaa gtgaatttga gctcagatct
     7081 taaaagcgtc tttttcaatg ctttgagaga aatattgagt gaaaatccat cgatgtacga
     7141 gccgaatgca atttatccag cagctaataa aaaggtacaa gaagtggtca aacataaaat
     7201 ggaaattttg aacacagtag gcaaggctag tttatactaa aaataaaaat gtgtggtcaa
     7261 gagggtatga caatagtctt gtcataccct cttaaccaca cacgatgttc agaagtagtt
     7321 ttttcgaaaa atttcttctt atttctcgaa gccaatcact tctgtcacaa cctctttttt
     7381 cctgttacta tataaagtaa atggggtgat tttatgttga atgaagtgga gaatagacag
     7441 caggagattg tgaattatct aaaaaacaac caatttgcaa gagtcaccga tttgatcgag
     7501 ttagtaaatt atagcgaagc cacgattaaa cgtgaccttg tttttctgga aaaaagtgga
     7561 ttgatccgta gagttcgcgg tggcgccatg cttattgaca atcgaaaaat tgacgtgcct
     7621 tacctgatga agatcaccaa attagaagaa gaaacagaaa aacgtaagat tgcagataca
     7681 gcttcttctt tgattcgaga tgatatggtc ttattcttgg attccagtac aacctcttta
     7741 cacttagtca gaaacctagc aagatttgat ggtttgcaga tcattacgaa tggcatcgtc
     7801 acagctgcta tgttgagtga atatacaacg gcaaaagtat ctgtattggg aggggcaatt
     7861 gttccgaaac gagcaacaat caacggttca aaagcctata acgatgcatt gtcatattat
     7921 gcggatttag cgataatgag ttgtcgcggg ctagatttca gccaaggagt aacagaaact
     7981 catgaaggag aggcaatggt caaaaaagct tttagaaaac aatcagaccg gcttgccgtt
     8041 ttagttacac agcaaaaaat tgcacataaa ttcatgcatc aagccattgc ttttcatgaa
     8101 atcgattatc tggtgacaag taaagaactg acaaaagaac agtatgcgaa agtaaaggaa
     8161 caccatatcg aatgtctgta cggataaatt attacgtgaa tgttctaagt ggcttcctca
     8221 tttttagata ggaccttatc tttgctcgct tctctcagcc gatggtataa taatgaaaag
     8281 gttatcttgt tagataatct tactttttta tccaggagaa aggagtgcag aaaatgaaac
     8341 atgtgaaggc gttggtagtg aaggcaatca tgatctgggc gattctgtgg gtcgtgctga
     8401 caggtttata cggtgttagc tttatggatt caacgattgt tggggtaatt attgtagtca
     8461 tgatctatgt gctaggggat ctgttgatct tacgtaaagt agggaacatc gcagcgacga
     8521 tcgcagatgc tggttcagct gcagttatct tgtggctcta tctatatttt atgaatgata
     8581 cagtcgatat ttggatgaaa gtattgattc ctgctctatt gatcggtgtg gcagaatggt
     8641 tcttccacaa gtggttgcta agtcaaggca tcgtaccaga tgaacggaaa atggaataaa
     8701 tcaacagtta aactattctt atcgataaat gaaagcttta aaaataaata gatgtttaat
     8761 taagaataat cataaactat ttttactcaa aaaaccgctc agattatttt cagatctgaa
     8821 cggttttttt tatctattca tcaagatcaa atttcatttt tcaatgattc aatcaattcg
     8881 ccctctgctt catgactatg ttttcggcca ttaacaagat agagtgctgt acatatcaat
     8941 ggaatgactc cggcaatcac atacaatcct ctaaatgaag taagagattt tagcatcccc
     9001 atcaaataag gaccaacgcc taagcctagg tctaaaccaa taaagtaggt agataaagca
     9061 atactgattc gatgttcgcg gacgattttt aaacaaacag cctgtccatt tgacataaac
     9121 gtaccataac ctaggccgac taatgctcca gagataagca atgtcacact attcgtcgtt
     9181 acactcagta aaaataatcc tgctgttaag aataaatagc taggatacat cacgtattgt
     9241 tctcctttga catcgaaaat ccttccagtc aatggacgtg taaatgtgat gaccaaagca
     9301 tatacgacga agaaaaatga cccagcagca actaagtgga ttacttttgt ataagaagat
     9361 aaaaatgata aaacacttga ataagaaaga cccatcaaaa aggcaattcc tgtaatgaaa
     9421 agtgctttct tttcaataaa actattgata ttccatgagc gaagagatat tttttgttct
     9481 tcagataatg gaatattttt tacaggaaag acaaggcagg caatcgtaac aaagaagacc
     9541 agtacaattg aaaaccagat aatgaaacga aaatcagtca tattcagcaa aatcattcct
     9601 aaaaatggcc caacagcggc agctaaacta gtacttaatc cataataatt gatgccttcc
     9661 ccttttttag atgcagggat atatgcagta acgatagcat ttgtagctgt agaaactgta
     9721 ccgtagccga atccattcaa gaatcgaact aaatacaatg ccccgatagt tggcgtataa
     9781 agatatgcta atgttgaaag gagatagaag atcgcgccgc ctcttaaggt aaacttccga
     9841 ccaaatagtt ctaactgttt ccccatgaat aagcgtgcca gcaatgttcc gatgatatag
     9901 atcccagaag caaaacctgc ttggctcaag ctggcgtgta aggtgtcttg cgcaatgact
     9961 gccatgatga ccatcaacag ataataaatc aggtagacaa taaaattgat caatgtgatt
    10021 ccaataaaat atttattaaa taatttcttt tccatgtatg ttatacattc cccttaagct
    10081 gaatatgctt ttaatttttg tatttgaact tctacatcgt gataattgga tggatagcga
    10141 tcaacaagtt ggcgaataaa taaatctctt gtagacataa acagatttaa aaattcttcg
    10201 cattcgtttg ttggattctc atccactgct ttgatcaatt cgatcgagtt ttgatacgca
    10261 tcatttaaaa agactaaaga agctttcata tcaaatcttg tttgatatgc tgtttctaat
    10321 cgattaatga ttgtgttcat ccagtaattc atgttttttc cctctattct taaaaaattt
    10381 tacttctgca gtataacgaa taaaaaaaag gaaaaaaatc gcataagtga tggaaaaagg
    10441 tacttaacaa atgttaatat cattagtaaa atacctttgg actttaaaaa gttttaaagt
    10501 actgtatgat ctttttagat gaaagtttgg atgggggagc atcattggat agtagagtct
    10561 taaaggaata tttggataaa aatgattttc cgattatacg caaaggatat cataaatatt
    10621 tgacattcga agggatggaa gattcccata cgtatatctt gaaagaggga acggtaaaag
    10681 caagtgttat ttcaaatgat gggagagaat ttaaccttcg ctatatcaaa gatcttgaaa
    10741 tcgtctctct tttaaaagac gaatattcac aattcatcga ttcgccgtat aatattcgag
    10801 ttgaatcaga atatgcagag ttttatcgaa tcaatcgtgt gcgcttttgg caaaatatca
    10861 accaagaccc aaagctacaa cactatgtaa aagagtatta ccgctatcgt ctgctttatt
    10921 cgatgaaaaa aatgcagcaa atgctattaa acggaaagct gggagctgtt tgtacgcaac
    10981 tttacgaatt atatgattta ttcggtgtgc ggacagaaga agggatgctg atcgattttt
    11041 cgatcacgaa tgaagaaatc gccaaatttt gtggcatctc aactgctagc agtgtaaaca
    11101 gaattttaaa acaactgaaa aaagatggta ttatcaccgt taaagaccat catatcgtta
    11161 ttttgaatat ggacttgtta gaagacaata ttatcttttg atgaacaaag aggttgtgaa
    11221 atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    11281 cgttttttga gctaataccg agggtcagct gattgaacac cgtttaccaa gaggttgtga
    11341 gcctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt tcatgtttga
    11401 tgaatattgt gacttgtgac cgaggagttg gtaggagaac accgtttacc aagaggttgt
    11461 gaaaaagctg ttctgcatca agtaataaga agagccaagt aaaaacagtc tcttatgttt
    11521 ttaacttggc tggacttatc accgtagatg taagcttttg aacgccgttt atttgcaaca
    11581 aaaggggctg tgacacgatt tttgtcacag cccttttttt gcgaacattc atttagcaat
    11641 ttttagaaac ttctgccgcc accgccaaaa gtcccgccac cgctagaatg ggtagtactt
    11701 ccgccaccgc cgcttccagg aggtgggctt ttggggattc ttcttgtcgt gacaaaagaa
    11761 tttgtcagac gatcggtttt ttcagttaat ttgatactgg atttttctcg gaatggatat
    11821 ttgtagatgc ctgatttcaa ttgataacgc gaaacagtca caaaataaaa gacaagacta
    11881 aggataagag ccaaaacaac agcaatgatg atttctactg tcgtcaaaac cttatagcgt
    11941 gtaattttgc cagtttcttc gtctacacga taatgtcctc ctggaactcc gtcttccaca
    12001 tatgttgctg atttagacag gtaagctttt gcagcattgt aataggcagc atttgtcatc
    12061 tgatcataaa catcatctaa gatcgaatcg attctgctat cagtcaaata atcgatcata
    12121 ttaccagaag tcgaaatgta gatttcacgc tgtcccatat ctaatagcaa tactgagcca
    12181 ttttgatcat ttccgactgc gtctcttaag taattatcag caaagtctct tggttcttct
    12241 gaattagatg tagtcgttac gatcaagact cgtcctttga ttttttcatt cagcttttct
    12301 gcctgttgtt ccaaagattg gatttgatct gttgtgaata acccagcctc atcatttacc
    12361 gtagctgtat ctgcatgagt agccaccggc cagccaacac ttactagcaa aagaaagaat
    12421 aaaaaaaact ttttcatcat atcaagtaac ctccgatcag aaagaggatc gctagcccag
    12481 cgaaaatccc aagagcggtc agtcccaatt ttttctggct gatcggcagg acaccgctga
    12541 cctttcctgt ttgtccattc attgcgtagt agtagacttt tttacttgag tcattgcttc
    12601 gataagtaac aagccatacc ggcagtaata cgtaattatt tacttcgttt gtgattgaag
    12661 cagaagttcg cacaccggtc aatgttgtat atccatttgc ggtatctttt aacagcttct
    12721 ctgaatagtc ttttaattca gattgaatct gtgatttgat tgcctcatat tcgatatctc
    12781 ttttttctgc ttggaacccg gctaaatact ggcttttaaa atcaattgct ttatctaacg
    12841 ggaaaggttg cacgccttcg accatctttt gttgcgtatt ttttgataag gcatttttta
    12901 ctagttcttt aaaagaaagc ttgcctgctc ttgaaacagc aaattgcttc gtttctgtgt
    12961 attcgatgtc acctatccgc catatgcgga taactgtacc attcgcctgc atggcaccat
    13021 ctacttcagc atctgtagcc caataaggga aatacacacc tgtcaactta tcgatttgat
    13081 ccttattgaa aaaagctttg ggaacaaacc attttttctt ggtccaagcg agaaatctgt
    13141 tgattgcttc ttctttctcg acagcaaaag gaagtacttt atttggcaag aattctccgc
    13201 ttaaacgtcc cgatagtacg acgggattat ggcagtagta acaataagtc gcagcagttg
    13261 tcgcatcagt cacgatctct gcaccacagt tgggacaaag aaaaagatct atcgctgcgg
    13321 tattatctgc agattcgtcc gctgtatctg aaaaaccgcc tgcctcgtca accgctttgc
    13381 gctcggcttc attcatatcg gcaagctgtt cttgggctgt aaaagtaaaa tcttcggtag
    13441 gtgaaggttc ttctttgctt tcttcgccag ccatacgcgc ttctttttgc ttttgctcat
    13501 attggctgac ttcttcttct gtatagatat tcaggcagta ttcgcaatga aatttttggt
    13561 ctttgggatc aaaagtcaac ggaccgccgc aattaggaca tttatgtgta agaacatcca
    13621 tcagctgcac acctttctaa ataaattaat taacggggat tcctttaaac ggtttaccac
    13681 agtttggaca gaatttaggg atgccgttgg aaagatccac gatctcattg cattcactgc
    13741 atcgcatttg tagcttaggc tgaacgtttt ctacaggttt ggcagctccg cagttggaac
    13801 agaattttcc ggaattttct gtcccgcaaa ctggacatgt ccaagtatcc gcatttcctt
    13861 gtgcagtttg ttgattttgt ttttctgctt gttgtttgat ttgttcttgg ttattttgag
    13921 ctgcttgaga aagataagaa ccatttgcat tcattcccat acctacaccc ataaagccgg
    13981 tcattgctcc tgcttcattt tttccggctg cttccatacc acgtgcaatg gaaccttgca
    14041 cgtatccttc acgtacgcta ggatcaccta gcatcgcccc ttcgttacgc atattgatca
    14101 gtttcacaga atcatcggtg taagaaatgc tggctacagc cacagaaaca atctccatcc
    14161 cacgtaattc acgccaagaa tcatccaatg cagtatccat gtatttgctt aattccaaac
    14221 tcttagatgg tacataagaa atccgttgac catctgcaga catctgattg attgctgatt
    14281 gtagcgctgt taggaattca gctaaatact gctcattgat atcattgatt tccacttgtg
    14341 ttttgttttt agggattgcg tttgtataaa ataaaatcgg gtcaacgaca tgaatagaat
    14401 aagtgccatg tgcccgtaag aataattctg cgttatagaa attgtcaaag tagttcaacg
    14461 gcgcgcttgt accgaatttg attcccttta tttcttggag attgatataa aaaacttgtt
    14521 gtttctgcgg tgtgacgccg ccaaatttga atcggtcaaa agtttcagca attgcttctt
    14581 tcagtgttcc attgaacatg gaaggagctg cgtcattttt aatggtgtaa taaccttcct
    14641 cagcggtata atcgatgatt tttccaccat cgaccaaaag catcatcatg tttggataga
    14701 catggacgac tgttccgtct gttaaaacat cttctgttcc tttacgattg gagccacgtt
    14761 tgtcatcttt gcggactttg acaccttttg tcatgaccgt tgtatcactc atattgtctg
    14821 gttcgatgac ttctagccat tgatctgcca gaccgccgcc aaccatactt gttgctgctt
    14881 tgataagacc cataaaaaat tcctcctctg attcagctag tgccaaacaa ttattgttta
    14941 tttattatac cataggaaga tgtgaaactt ctcggactag agacggaatt gaaatggaaa
    15001 attgcgtatt gtttaaatac ctgaaaaagc cgagaaggat agtagttttt ttacctaata
    15061 tcacgtataa taagagatag tgaatttttt aaaaaagggg gatccttatg gcatatcaag
    15121 cactttatcg agtctggcgt tcgcagcgtt tcgaagacat cgttggacaa aaagctgtga
    15181 cacagacact aaaaaatgct attgtttccc acaaaacctc acatgcctac ttgtttacag
    15241 gacccagagg aaccggaaag acgagtgcag caaagatttt cgcaaaagca atcaactgtc
    15301 cgaacagtaa agacggtgag ccatgcaacg aatgcgaaat gtgtcgatca attactgctg
    15361 gtacgcaaga agatgtgatc gaaatcgatg ctgccagcaa taacggagtc gaagaaatcc
    15421 gatttatccg tgatcgggca aattatgcgc ctacaaaggc agcgtacaaa gtgtacatta
    15481 ttgatgaggt acatatgctt tcaacaggag ctttcaatgc cttgttgaaa acattggaag
    15541 agccaaaaga aagtgtcata ttcatcttag caacgaccga acctcacaaa ataccagcta
    15601 cgatcatttc acggactcag cgatttgatt ttaaacggat caatacggca gatattgtcg
    15661 aacatcttga atttatcttg aaagaatcga ctatcagcta tgaaccagct gcattgaatg
    15721 tgattgcccg ttctgcagaa ggcgggatgc gggatgcatt gagtattctt gatcaggcaa
    15781 tctcgtttag tgaaggaaca attactttag atgatgccat gcaagtgaca ggtagcttaa
    15841 ctgacgaaat gatggacagt tacttgagtg cttgcgtgaa aaaagaagta ccagaagcat
    15901 tggaaacatt gaaccaaatt ctcgcagccg gcaaagaatc acgtcgcttt ctagaggata
    15961 tgttacaata ctgccgtgac ttgctgatgt atcagcaagc acctaaccta gttgaggaac
    16021 aatcgagtac gatcagaagt acatttacag aattggcaca aactgtttca gcggatcagc
    16081 tgttttcaat gataaagatt ttgagcgata ctcaaaatga aattcgtttt accaatagtg
    16141 ccgctattta tttggaagtc gctacagtca aacttgcaca gctaccgtct gcttctgttc
    16201 cttcagcaga tgtagcattt gcagatgatc agatagtaaa acagctacaa caagagatta
    16261 aggaactaag aaacgaaatc aaggaactac gcagtaatac aggaattgag aatacaaaag
    16321 tacagccggt acagcaaaca aatcataaaa aacagacaag tacctatcgt gtaccgacgg
    16381 aacgagtata taaagttctg cgggaagcga caagacagca tctgaaccaa gtaaaagaag
    16441 tatgggaaga cttgctgatg agtctatctg tcactcagcg agcaatgctt aaagcaagcg
    16501 aaccagttgc agctggacct acaggactag tgattgcgtt tgattatgag attgtttgtc
    16561 aaagagcagc aaatgacgaa gaattgcagc tttcggtaca caacaactta agccgaatga
    16621 tcaaagatta tgcgcctgat cctgtttata ttacaagaga aagctggcca gaacttcgaa
    16681 gaaattattt gacgcaggca caagaagagg gaacagaaaa ctcttttgct tctgaagact
    16741 cagaaggaga aatcgagttg cttcctcaag aacaagaagt accagaagaa acacttgtag
    16801 taaaagcaga ggaactgttt ggctccgaag ctgttacagt ggtagatgat taataaaaag
    16861 gagtagataa ttatgatgcg tggaatgggc aatatgcaag gcatgatgaa acaagtacaa
    16921 aaaatgcaaa aagaaatggc agaagcacaa gagctgctaa ataaaacaga gtttacaggt
    16981 aaagccacca atgaattggt caaagcggtt tttacaggcg atcgtcgcat gaaagatatc
    17041 caaatcaatg aagaagtcgt tgatcctgat gatatcgaga tgcttcagga tcttgtcgta
    17101 atggcagtca atgatgcgat cgcacaaatt gatgtggaaa ctgagaaaaa attagggaaa
    17161 tacgcaaaag gtttgccagg attttagttt taaactgaag gagtaatgaa tatgcaatat
    17221 ccagaaccaa ttgcgaaact gatcgacagc tacatgaaac ttccaggaat cggtcagaaa
    17281 acagcgacaa gactcgcctt ttatacgatc gatatgaaag atgaagtagt aaatgaattt
    17341 gctaaatctt tgttaagtgt aaaacgcgat ttgcattttt gtagtatctg cggaaacatc
    17401 acagaagaag atccctgcga aatatgtaga aatccttcac gtgatcgaag cgttatccta
    17461 gtggtcgaag agccgaaaga tgtaatggca atggaaaaaa tgcgagaata tcacggactt
    17521 tatcatgttc tccatggtgt gttatcaccg atggaaggga caggtccaga agacatcaat
    17581 atttcttctc tgttgcagcg gctgcatgat gcagatgtaa aagaagtgat tatcgcaaca
    17641 aatgcaacaa cagagggaga agcaacagct atgtatctct cacgtctgat caaacccgca
    17701 ggtatcaaag tgactcgtct agcacatggt ctgtctgtag gctctgatat cgagtatgca
    17761 gatgaagtta ctttattgaa agctgtcgaa ggaagacgtg agttataaat ttctaattaa
    17821 gtgttttttc agatacaata taaaagcagc acaagagatg ggggcaacaa tgtgaacgga
    17881 ctatttatta caatcgaggg gcctgatggt gcaggaaaaa ccagcatctt gaatgaactt
    17941 taccctcttt tgaaagaagt agcaaaaaca gaaatcattc aaacaagaga accaggaggc
    18001 attccgattg ctgaagagat ccgagcagtg atccttgatc caaaaaatga tcggatggat
    18061 gaacgtacgg aagcactgct ttatgcagca gcaagaagac aacatctagt cgaaaaggtt
    18121 ctgcctgctc tggcaaaagg gaaaatcgtt ttatgcgata gatttgtcga cagttctctt
    18181 gcttatcaag gtgcaggccg acgcatcggc gttaccgaga ttgctcggtt gaatgagttt
    18241 gctacagaag gtactacgcc ggattttacc ctttatcttg acgtagattc agacaccgga
    18301 ttaagaagaa tcaagaaaaa cagacaaaat caaatcgacc gtttagattc ggaaggtttg
    18361 gaattccacc agcgtgtacg tcatgcttac ttaaagctgg cagaagagaa tccggaaaga
    18421 atccacaaag ttgatgcaag aaaaagcttt gaagaagtgc tgcaaacaag ttatcataca
    18481 attattgaac agtatccgca attttttgaa aactaggaag gtgaacgttt atgaaaatta
    18541 ttttagcgat tatccaagac aaagacagca accgtttatc aaatgaattg attgatgcaa
    18601 atatccgtgc cacaaaacta tcatcgacag gcggcttcct taaggctgga aacagtacgt
    18661 ttatcgtggg tattgaagat gaccgtgtag aagaagcatt ggaaatcatc aaaaaaacgt
    18721 gtgaatcaag aaagcaattt gtatctacac ctgttacact ggatatttcg atggatgggg
    18781 gcgtccctta tccagtagaa gtagaagtag gcggtgccac agtcttcgtc ttacctgttg
    18841 aaggattcca tcaattttag gaggagctga gctatgaatc aagaaataaa cttggctcaa
    18901 atgcaaccga tcgtgtatca gcagcttcaa cggagttttg agcatgggcg tatcgctcat
    18961 gcttatcttt ttgaagggga gaaaggaaca ggaaaacatg aagtaggtat atggcttgca
    19021 cagcacctgt tctgtaccca aatgaaagac aatctgcctt gtggaaaatg caacaattgt
    19081 cagcggatcg aaaagaaaga acatccagat gttttggtta ttgaaccgga aggacaaacc
    19141 atcaaagtcg atcagatcag acgtttgcag acggaattca gtcgcagcgg ttacgaatca
    19201 agaaaaaaag tttttttgat caaagaagct gaaaaaatga atagcagtgc ggcaaacagt
    19261 ttgttgaaat tcttggaaga gccccctgga gattttttag cggtgttaga aacagatgca
    19321 gtaggaagaa ttctccctac tattcaatct cgttgtcaag ttctgcattt tcaagaactt
    19381 tcgaaagaag cactgatcca tcgattgcaa gaagagcaga ttccattgga aaaagcaaaa
    19441 cttttggctt tcttaacaaa cagtttagga aaagcagttg aaatctctca agatgaatgg
    19501 tttaatgatg ctaaggactt gatttatcaa tggttcgttt acttgcaaaa gcaagatact
    19561 caagccttta tctatgtcca aaaaaaattg gtaaaatcat ttaaagaaaa aacacaacag
    19621 tttacaggat tatctgtctt gatgttttat tatcaggaag cacagcgtca agcagtagca
    19681 aacggcgaat atggcaaagt gaaacagata aaccagataa ttgaacggat attgatcgca
    19741 gagcagaaac tacgcagcaa tgtcagtttt caggctgttg cggaacagtt cgttttgcat
    19801 acgatttttt agcttgccgc aaaggatgat gatcaaggag gatggaaatg gtagaagtag
    19861 taggtgtccg atatagagat gcaggtcata tctattattt tgcaccagga gactctgaat
    19921 atgtttatag tgacaaggta ttagtcgaat ctcagcaatc cttacatatt gctactgttg
    19981 tccttcctaa aaaagagatt gatcccaatg accttcctga agctatccgt ccgatcttgc
    20041 ataaagcgtc acaagctgaa ttagaaaaag acaaaaaaaa caaaaaagat gcttatgatg
    20101 cattgaaaat cgcaaaagaa aaagtgcagt tgcgtgagtt ggaaatgaaa atggtcaaag
    20161 ccgaatacac atttgatcga aataaattga ttttttcttt cacagcggac gggcgaattg
    20221 actttagaga attggtaaaa gatctggccg ctgtatttcg tactcgtatt gagcttcgac
    20281 aaattggtgt ccgcgatgaa gcaaagataa taggcggaat tggtccatgt gggcgtccat
    20341 tatgctgttc ttctttttta ggcgatttcg tgcctgtatc aatcaaaatg gcaaaagatc
    20401 aaagtttatc tttgaatcca acaaaaattt ccggtttatg cggacgcttg atgtgctgtc
    20461 tgcaatatga gagcgaagaa tacgagacag cgaaaaaaga actccctgac tttggaaaag
    20521 taattgctac acctgatggt aaagggaaag tcgtagggtt gaatctgctt agccgtatcg
    20581 ttagtattcg gctgactgga cgggaaaatc ctgtcgaata tgaatgggat gaattgaaag
    20641 aatttatcca agtaggtgag atgaatggat aagagatcat tatacgacgg attgaatcaa
    20701 ttagaatcag agttgagaaa tacattaaca gaacttgttg aaatgaagca tgctttgcat
    20761 gagatcgtag aaaaaaatac aactttggag atggaaaatc aacgtctgcg ggaacacctg
    20821 cgggaattga accaagttgc acaagctcca gctgaaaatg tgaagcagga actttctaag
    20881 tctcgtatga atcttgaaaa aatttatgaa gaaggattcc atgtctgcta cgatttatat
    20941 ggatcaagaa gggacaatga cgagccttgt gcgttttgtc tagaagtgat ctatcgcgaa
    21001 agcggtcgat gaccaataac aagaggagac tggagcggag aaatactgct ccagtctttt
    21061 tattaaggag attgtgacag atgtataatc aaaaaagttt caagggaaat ccggtatatg
    21121 gggatttata tcttgtaccg acgccaattg gaaatttaga agatatgagc tacagaagtg
    21181 ttcgtatgct gcaagaatcc gatcttatcg caagtgagga tacgcgtaat acacaaaagt
    21241 tattgaatca ttttgaaatc catacacccc aaaaaagttt acatgaacat aattataaag
    21301 aacgtgtccc tcaattgatt gctttgttaa aagaaggaaa aacgattgct caagtcagtg
    21361 acgcaggaat gccttccatc agcgatcctg gacatgagct agttttagcc tgcataaaag
    21421 aagggatttc tgtgatctct atcccaggac ctacagctgg gatgacggct ttgattgctt
    21481 cagggctagt gcctcaacca tttttatttt atggcttcct tccacgtaag aaaaaagaac
    21541 agaaagaaac attgaaaaag ctaaaagaac agacagttac gctgattttt tatgagtcac
    21601 cttatcggat cactgctaca ttgacgaatt ttttagaaat ttttggaaat cgccaagtcg
    21661 ttttgtgtcg ggaattgaca aaggtccatg aagaatacct tagaggaagt acggaagagc
    21721 tactcgctta tatcgaagaa catccagtaa agggtgaatg ttgtctgctt gtagaaggga
    21781 atttgttcga aatgccagtg acggaagttg atgaagagtt gggaacgtta aaggaacaag
    21841 tagaagcaaa gatcgcttca ggcgaaaaac caaatgctgc tatcaaagca gtagcgctga
    21901 aaaatgggct gaaaaaacaa gaagtctacc gtcagtacca tgaattagac tgagatagta
    21961 taagttgtct ataatgaaag actaatgagg aaatcagcta ctaacttttc aaatctcttg
    22021 aacgcgaacg aatgttcttg tataatgaat aagaggtgag gatgatggag ctgcaatttc
    22081 ctttacataa tgatacatca agaaaaatca ttcatattga tatggatgct tttttcgctt
    22141 cggttgaaga acgagatcat cctgagtttc gcggacatcc attagtaatt gccagacacc
    22201 ccagtgacac tggtgggcgt ggagtagtga ctacagcaaa ttatgaagca agaaaattcg
    22261 gaattcattc agcaatgagt gcacaaaaag cgtatgagct ttgtccgcaa gccatcttta
    22321 agcctggcga ccatcataaa tacagcgata tttccaaaca agtacgggaa gtattctatc
    22381 gttatacaga tattgtggaa ccagttagta tcgatgaagc gtatcttgat gtgaccgaaa
    22441 ataaaatcaa ttgcaagtca gccatcaaaa tcgcccgaat gatccaagca gatatttggg
    22501 agagtgtcca gctaacatgt tctgcgggag taagttacaa taagtttttg gccaaattag
    22561 catcagacta tcaaaaacct agaggaatca cggtagttgc ccctcaagac gctgtgccat
    22621 ttttaaaagc tttgccaatt gaaaaatttc atggggtagg gaagaaaact gtacctagaa
    22681 tgcatgagct agggatttat acaggaaaag acctatatga atgcacagaa atgatgttga
    22741 ttcgaaattt tggaaaaatg ggctattctc tttatcgtaa agtcagagga atccatgatt
    22801 ctccagtaaa cgttacaaga gagcgaaaat ctgtgggaaa agagcatact tatgggcagc
    22861 cgttgcaaac ggaagaagca gtgctgactc agttgcgcca actagctgaa aaagtagaag
    22921 aagcactgag aagagtccaa aaacatggga aaacagtagt tttgaaggtc aggtacacgg
    22981 actattctac tgtgaccaaa agagtgacat tgccagaata tatctataaa aaagaagcac
    23041 tcttttatca agcaagcctt atatgggagg aaatcttggg agtagaaaaa ggaatccgat
    23101 tgttagggat tactttgacg aatctggacc cgatgactta tgaaaacatc gtccttccat
    23161 tatgggaaaa tcaagaaata taaaaacagc tttgagagtt gaaaccggaa atccgttttc
    23221 tctcccaaag ctgtttttat atcaagtctt gctttttgat ttgttcatag gtttctgttg
    23281 catccgtaca tttttcccaa atcacaactt gattggattc taaagatttt tggacgtcaa
    23341 tgatccgctg gttgctactt ccgcgaaatt gtagttttag gtcacgtttg cttaattcaa
    23401 agcgtccgtc aacaaggata tcgatttgcg aaaggagttc taatttatct tctgaatctt
    23461 gtagcaattc atcaaaggca tagcctgtcc atgaccagat atcttttgtc gtaccgaact
    23521 cttttcggat acgtcgtacg acttgcagac aaacatctgt attcaaaaaa ggttcgccac
    23581 caagtaaagt taatccttgc acgtaatcgt tgcgcaagtc agcgatgatt tgttcttcta
    23641 attcaggtgt gaatggtttg ccatatcgga aattttgtgc agctttgttg aagcagcctt
    23701 cgcaggcaaa taagcagcca ctaacgtaaa gactgttgcg gacaccttca ccatcgacaa
    23761 aattaaacgg tttgtagtca gcaatatagt tttggctgta gtcttttgct aaccattctt
    23821 ttggttttgg atttctcata aaaaaacttc tttcatttaa ggttcaaaga tatagctatt
    23881 gaagataaaa tctactcgac cacataaata agaacaaact gacctaaatc cattgttcaa
    23941 gaaagcttgt tcttattttg atagagggcg ttcaaagata caaagccatc gaaagagggt
    24001 ggtgaaaaaa gacttgtcat agcctcttat tctgaataaa cggtgttcaa aagcttgcat
    24061 ctgcagtaat aagcccaact aaacgaaaaa tatgaggaac tatttttgtt tagctattct
    24121 tattacttga tgcaggacag ctttttcaca acctcatttc atgtgtttca cacgtgagga
    24181 aatttcttta tggcggccat gtaccatcgg acgagcttgc ggatttccta aatagccaca
    24241 agtacgtttg actacatcac atgtttttgg atcatgattt ccacattctg gacattcgaa
    24301 ccctcgttct gtcggtttga aatcaccttc gaaaccacat tgatagcaat gatcgattgg
    24361 cgtattcgta ccaagatacc cgattttgtc atatgcaaaa tcccatacag cttctaaagc
    24421 tttcggattt tgttggagca tagggtattc acagtagtgg atgaatccac ctgaacaata
    24481 ttttggataa tccttctcaa attccaattt ttcaaatggc gttgggtttt tacgcacatc
    24541 atagtggaaa ctgtttgtat aatattcttt atctgtgata ttttcgatga tgccgaattt
    24601 ttctgtatcc aaacggcaga aacggtcagt tagactttca cttggagttg aatagacact
    24661 gaaatgataa ccatattcat ccccccatgc atctgcatta gcttttaatt ctttgacgat
    24721 atcaagtgtg aaagcttttg cttctgggtt tgtttcccat tcgccaccga agaaagctga
    24781 agcaacttca tacaatccaa tgtagcctaa cgatacagta gctcgacggt ttttgaataa
    24841 ttcattgaca gaatcggtac gtgaaagacg tttcccgaaa gaaccataca tatataaaat
    24901 tggcgcattt gctggaatgg cttctttaca acgttctaca cgatagagaa gcgcatcttt
    24961 catgatttct aagcggtcag acagtaactg ccagaattta ttctgatctc cttttgattc
    25021 gatcgcgatt cttggcaaat tcaatgtaac aacacctaaa ttcattcgtc cgacatttac
    25081 ttcttgacca ttttcgtctt tccacccttg caagaatgaa cgacagccca taggtacttt
    25141 gaaacttcca gttaattcaa cgattttatc gtaattcaga atatcaggat acattctttt
    25201 cgttgcacat tccaatgcta gttgtttgat gtcgtaatta ggatcttcag ggtttaagtt
    25261 cacacctttt ttcaaagtga agatcagttt agggaagata gctgtccgct tttcactccc
    25321 taatccattg attcgaatct gtaaaattgc tttttgaatt tctctttcaa accagttttc
    25381 tcctagtcca aatcctaatg aagtgaaagg agtttgtcca tttgaagtaa ataaggtatt
    25441 gatttcatat tccaaacttt gcatagcatc gaagatatct tttttggttt ttgcttttgc
    25501 atattctttt tggcgttctg gcgaatcgat ccattcttcc gcgtctttta gatgtttttt
    25561 atagttcaat tctgcaaaag gagcaagcaa ttcatctgtt cggtcggcag aacagccacc
    25621 atattgactt gacgctacat tggcaataat ctgtgagatt tgtgctgtgg cggtctggat
    25681 agattttggc gattcgactt cggcattgcc gattttgaac ccattgttca gcatgccttt
    25741 gaaatcaatc aagcagcaat tcgtcatcgg tgtataagga tgataatcca aatcatggta
    25801 atggatatcg cctttttgat gcgcatttgc tatatgtggt ggcaacattt tcagtccgat
    25861 tgattttcca acgatcccgg ctgtcagatc tcgctgtgta ttgaaaacat tactgtcttt
    25921 atttgcgttt tcgttcacga cagtttggtc cttgttgatt agcttttcaa tcgaaaagtt
    25981 gatatctgtt gctttactgc gagcaaaatc tcgtttggta cgataattga tgtactcttc
    26041 tgctaattca tattcgtttt tttctagtaa tatatgttcc acgatgtttt gaatctcata
    26101 gattttgaca ttttcagcaa accggctttt gatttcttgg acaaccaagt ctgcaatcat
    26161 ttgaattttt tcataagtaa gtggtgataa tgagccgtga attttctttt ctgctttgat
    26221 cagtgcttca ttgatctttt gttcatcgaa atcaacatgg cggccatcgc gtttgatgac
    26281 tttcatctgg gatgtctctg gctgctgagt aacgacagtt ttttcttcat aatttacttt
    26341 cattatcttc aactcctgct aaattgttct tgtctatcat aaaacaaaat cggggaagga
    26401 gcaactaaat atcggctata tatagtattc aatgtgctgt ataattggat ttctatacta
    26461 tatgtagtgt atattttatt gtgaaaaaag gtgaaaactt gtagctggtg gatttgtttt
    26521 ttgcatattt aattttgctg aataaaataa ttgaataaaa ttttttattc agtccaagta
    26581 aaagtgacaa aaacataaga gcaaattatt cgtagatatg ctactatgca aataaataga
    26641 aatgaaaaat gaaagaggag gagaagaatt ggaaaagatg atcgaagtga aacatctaag
    26701 taaaagctac ggcagcaaac tttctcccgt caaagcctta gataatatct cattcacagt
    26761 agaagaaggc gagtttgttg ggattatggg cccaagtggt gcaggaaaga caacgctgat
    26821 gaatatcttg tccacgatca atttgcctac gatgggcagt gtctcgatcc aaggaaaaga
    26881 aatcacaaaa atgaaaaaca gtgagctcag tgactttcgt cgaaaaaaac taggattcat
    26941 ttttcaagag ttcaatttga tcgataccct aaatgcaaaa gacaatatcc tattgcccct
    27001 agcagttgaa cggatgacta aagaggaaat ggaaaagaga gtaagacatg tagctcaatt
    27061 gttgaatata gaagaactgc tcaaacggta tcccgatgaa ctttctgtcg gacagagaca
    27121 acgaattgca gctgctagag cacttgttgt tcagccacaa gtgatttttg cagatgaacc
    27181 aactggctct ttggactcga agtcggctac agaattgttg aactacttga agaccatgaa
    27241 tcaaaaagaa aaagcaacga tactgttagt cacccatgat ccatatacag caagctactg
    27301 cgatagaatt ttatttatta aagatggtgt gattttttct gaagttgtca gaagagggac
    27361 aagaagagag ttctttgaga aagtaataga catgcaggcg acgattggcg gaggaggaaa
    27421 aatgaatgct gtataaacta gccgttaaaa gtttttttcg gcagtcacga ggctatcttg
    27481 tttatttttt cagtttgact ttatccgtaa tgatctatta ttccttcagc gcaatgacct
    27541 acgatcaatc tttagtcaga agagcaagtc aagatacttc gatagatggt ggattgagtt
    27601 ttggtggatt tatcattact gttgttttgc tttttttcgt attcagtgcc aaccgatttt
    27661 tcttgaatag aaggcaaaag gaaataggca tctatcagct ttttggtatg aataaactgc
    27721 agatatcagg tatatatgtg ttagaaatca tgataattgg attgttcgcc tgtatttctg
    27781 ggatattact cggtattatt ttttccaaac tcttttcaat gattttggtt cgaatgatgg
    27841 atatggatct tactagtcca ttttttatct ccattccatc agtagtagat actttatttg
    27901 cttttttcat catcctttta gcagtatctg tatattcaat atggaaaata tggcgctatc
    27961 caatgattcg aagttttgga gaaaaagaac aagcagatag ttcaacaatg cgcttcagga
    28021 caaggcaccg attactcgca gtcatcgggt tactactttt aacgagcgga tattttggag
    28081 ctagtcattt tagggaaatc acttcatgga tgatcagtta tcattatctt gatacattag
    28141 ttttcttttt tcctttttta atttttttcg tttgtgtggt cggtacttat ttgtttttct
    28201 gttatacatt tcggttattt ttgatcatat tgagtaaatg gaagatttat tactatggta
    28261 tcaatttttt gataatgggt aacacgcaag ttcatttgct caaaagttgg cgtacaaatt
    28321 ctctgattac agtcattctc ggaatttctt taatgacaat tggcggcacg gctagtatct
    28381 ctactatttt atcctaccag gagaaagttt cctccccgat ggactatcag tttgatgttg
    28441 agacagagaa aaaaatcaag ccgattctag cagaggaagg gcagaagata actaaagaaa
    28501 tcaccttgca ttataaagtc attgggagta tctatagtca aaatctcgaa gaacctatgt
    28561 ttcaaactgc taatctgatc acggaaacac aatatcaagc cttcagaaaa gttaatccag
    28621 atttgccgaa catttctttg aaatcacctg gtcatactgt tttgcttgat caaatgcaaa
    28681 ctctttttcg gaatatttca ttatatggat ctagtattgg actgccagat aatcaagaat
    28741 tgaagattca acaaatgctt ccaagtgtat taggtgacga aacaatggta tatctaagtc
    28801 ctgtactgat tgtagatgat accgtttttg aaaaagcaaa aggtgtggat tatcaagtag
    28861 tcaatgtaga tgtatcaggt ggaaataatg aaaaaataga tgaacgactg agcaatgacc
    28921 tgtcgataaa gtggctcaat gctgtttatt attcttatga tatcgtaaat ggaagtctac
    28981 aaggagaaat tagcacaaag aaacttccgg atgaccaaat gaaggaagca caatttagcc
    29041 aagaaagctc tcgtttgaat ttctcttcca gctattcgaa attgcgtgca gttaatcggc
    29101 gaatcggtat caatattttt gtcacactat ttgtcgggat gatcgtgatt attgctactg
    29161 gtagtatttt gacagtccgg caattagcag aagcagagga agaaaaagaa aattatcacc
    29221 tattgactaa gctggggatt ccacaaagaa ggatcagacg gatgatcttc gagcaaaacg
    29281 cacttacctt ctttccaccc atgatattag ggatcactca cgcagtattt gcaatcaatg
    29341 tcttcactca gtatataaaa actgccgatt actggttggc ttatcttgtg agtggcttat
    29401 tgatccttat ctatcttttg ctttatgtca ttacgagtca gttatattgt cggattatcg
    29461 aggaatgata tacggaataa gacaagataa taaatagaac taaattttga ataccattta
    29521 tttggaataa gaggttgtga cacgattttt gtcacaacct ctttattttt tatatttttt
    29581 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
    29641 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
    29701 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
    29761 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
    29821 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
    29881 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
    29941 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
    30001 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
    30061 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
    30121 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
    30181 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
    30241 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
    30301 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
    30361 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
    30421 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
    30481 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
    30541 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
    30601 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
    30661 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
    30721 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
    30781 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
    30841 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
    30901 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
    30961 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
    31021 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
    31081 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
    31141 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
    31201 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
    31261 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
    31321 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
    31381 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
    31441 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
    31501 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
    31561 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
    31621 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
    31681 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
    31741 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
    31801 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
    31861 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
    31921 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
    31981 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
    32041 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
    32101 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
    32161 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
    32221 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
    32281 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
    32341 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
    32401 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
    32461 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
    32521 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
    32581 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
    32641 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
    32701 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
    32761 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
    32821 aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac
    32881 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
    32941 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
    33001 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
    33061 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
    33121 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
    33181 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
    33241 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
    33301 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
    33361 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
    33421 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
    33481 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
    33541 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
    33601 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
    33661 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
    33721 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
    33781 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
    33841 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
    33901 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
    33961 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
    34021 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
    34081 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
    34141 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
    34201 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
    34261 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
    34321 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
    34381 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
    34441 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
    34501 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
    34561 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
    34621 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
    34681 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
    34741 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
    34801 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
    34861 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
    34921 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
    34981 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
    35041 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
    35101 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
    35161 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
    35221 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
    35281 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
    35341 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
    35401 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
    35461 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
    35521 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
    35581 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
    35641 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
    35701 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
    35761 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
    35821 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
    35881 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
    35941 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
    36001 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
    36061 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
    36121 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
    36181 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
    36241 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
    36301 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
    36361 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
    36421 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
    36481 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
    36541 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
    36601 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
    36661 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
    36721 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
    36781 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
    36841 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
    36901 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
    36961 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
    37021 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
    37081 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
    37141 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
    37201 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
    37261 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
    37321 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
    37381 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
    37441 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
    37501 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
    37561 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
    37621 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
    37681 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
    37741 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
    37801 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
    37861 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
    37921 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
    37981 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
    38041 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
    38101 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
    38161 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
    38221 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
    38281 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
    38341 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
    38401 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
    38461 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
    38521 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
    38581 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
    38641 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
    38701 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
    38761 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
    38821 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
    38881 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
    38941 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
    39001 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
    39061 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
    39121 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
    39181 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
    39241 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
    39301 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
    39361 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
    39421 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
    39481 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
    39541 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    39601 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    39661 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    39721 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    39781 gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaa
//
LOCUS       DAZAOG010000011        35546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000011 DAZAOG010000000
VERSION     DAZAOG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.011"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1104
                     /locus_tag="ACHDLD_000520"
     CDS             34..1104
                     /locus_tag="ACHDLD_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362279.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1119..1787
                     /locus_tag="ACHDLD_000521"
     CDS             1119..1787
                     /locus_tag="ACHDLD_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362280.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1807..2385
                     /locus_tag="ACHDLD_000522"
     CDS             1807..2385
                     /locus_tag="ACHDLD_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3362281.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2788..3699
                     /locus_tag="ACHDLD_000523"
     CDS             2788..3699
                     /locus_tag="ACHDLD_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3362282.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3955..4872)
                     /gene="rnhC"
                     /locus_tag="ACHDLD_000524"
     CDS             complement(3955..4872)
                     /gene="rnhC"
                     /locus_tag="ACHDLD_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN3362283.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5074..5511
                     /gene="zapA"
                     /locus_tag="ACHDLD_000525"
     CDS             5074..5511
                     /gene="zapA"
                     /locus_tag="ACHDLD_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN3362284.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5569..6116
                     /locus_tag="ACHDLD_000526"
                     /pseudo
     CDS             5569..6116
                     /locus_tag="ACHDLD_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            6231..8591
                     /locus_tag="ACHDLD_000527"
     CDS             6231..8591
                     /locus_tag="ACHDLD_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN3362285.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8678..8992
                     /gene="trxA"
                     /locus_tag="ACHDLD_000528"
     CDS             8678..8992
                     /gene="trxA"
                     /locus_tag="ACHDLD_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN3362286.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9174..10952
                     /gene="uvrC"
                     /locus_tag="ACHDLD_000529"
     CDS             9174..10952
                     /gene="uvrC"
                     /locus_tag="ACHDLD_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN3362287.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11097..11996
                     /locus_tag="ACHDLD_000530"
     CDS             11097..11996
                     /locus_tag="ACHDLD_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362288.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11989..13251
                     /locus_tag="ACHDLD_000531"
     CDS             11989..13251
                     /locus_tag="ACHDLD_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362289.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13414..15312
                     /locus_tag="ACHDLD_000532"
     CDS             13414..15312
                     /locus_tag="ACHDLD_000532"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN3362290.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(15611..16330)
                     /locus_tag="ACHDLD_000533"
     CDS             complement(15611..16330)
                     /locus_tag="ACHDLD_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN3362291.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    16520..16567
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16667..16999
                     /locus_tag="ACHDLD_000534"
     CDS             16667..16999
                     /locus_tag="ACHDLD_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362292.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16986..18989
                     /locus_tag="ACHDLD_000535"
     CDS             16986..18989
                     /locus_tag="ACHDLD_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN3362293.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19008..19478
                     /locus_tag="ACHDLD_000536"
     CDS             19008..19478
                     /locus_tag="ACHDLD_000536"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN3362294.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19499..20083
                     /locus_tag="ACHDLD_000537"
     CDS             19499..20083
                     /locus_tag="ACHDLD_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFN3362295.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20098..21084
                     /locus_tag="ACHDLD_000538"
     CDS             20098..21084
                     /locus_tag="ACHDLD_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN3362296.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            21074..21385
                     /locus_tag="ACHDLD_000539"
     CDS             21074..21385
                     /locus_tag="ACHDLD_000539"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN3362297.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21407..23188
                     /locus_tag="ACHDLD_000540"
     CDS             21407..23188
                     /locus_tag="ACHDLD_000540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN3362298.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23181..24557
                     /locus_tag="ACHDLD_000541"
     CDS             23181..24557
                     /locus_tag="ACHDLD_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN3362299.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24564..25199
                     /locus_tag="ACHDLD_000542"
     CDS             24564..25199
                     /locus_tag="ACHDLD_000542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN3362300.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25229..26584
                     /locus_tag="ACHDLD_000543"
     CDS             25229..26584
                     /locus_tag="ACHDLD_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN3362301.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26776..28170)
                     /locus_tag="ACHDLD_000544"
     CDS             complement(26776..28170)
                     /locus_tag="ACHDLD_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN3362302.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28262..29233
                     /locus_tag="ACHDLD_000545"
     CDS             28262..29233
                     /locus_tag="ACHDLD_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN3362303.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29230..30012
                     /locus_tag="ACHDLD_000546"
     CDS             29230..30012
                     /locus_tag="ACHDLD_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN3362304.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30181..30552)
                     /locus_tag="ACHDLD_000547"
     CDS             complement(30181..30552)
                     /locus_tag="ACHDLD_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3362305.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30686..31129
                     /locus_tag="ACHDLD_000548"
     CDS             30686..31129
                     /locus_tag="ACHDLD_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN3362306.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31172..31981)
                     /gene="yidA"
                     /locus_tag="ACHDLD_000549"
     CDS             complement(31172..31981)
                     /gene="yidA"
                     /locus_tag="ACHDLD_000549"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN3362307.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32000..33367)
                     /locus_tag="ACHDLD_000550"
     CDS             complement(32000..33367)
                     /locus_tag="ACHDLD_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3362308.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33351..34181)
                     /locus_tag="ACHDLD_000551"
     CDS             complement(33351..34181)
                     /locus_tag="ACHDLD_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN3362309.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34344..34781
                     /locus_tag="ACHDLD_000552"
     CDS             34344..34781
                     /locus_tag="ACHDLD_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN3362310.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34857..35477
                     /gene="rpoE"
                     /locus_tag="ACHDLD_000553"
     CDS             34857..35477
                     /gene="rpoE"
                     /locus_tag="ACHDLD_000553"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN3362311.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11606 a         6049 c         7648 g        10243 t
ORIGIN      
        1 gtataaaatg attatttcag gaggaggagt taaatgtttt tagcattaaa tgaaattatg
       61 cattcaaagt tacgttatgc gttagttgca ggggtgatgt ttttgatcgc ctacctggta
      121 ttttttttga ctgggttagc ttatggctta gctcaagata atcgaacagc agtcgacaag
      181 tgggaggcag attcgattgt gctttcgaaa gatgcaaatt caaatctagg gatgtccatg
      241 attactaaga agatagcaga agaagtcgaa ggtgggaaag tggcgtatct tgcccagaca
      301 cctggagtcg tgactagtaa agacagtaca gaagaaggaa aaatcaatgt tagcttcttc
      361 ggtatcgata aaaatcaatt catcatgccc aatcttgttg aaggaaaagc ttttgataac
      421 gatgatgagg cagtaggcga tattagtttg aaagaagaat atggtctagc cgtaggagat
      481 acagttaagt tatctggtag tgacaagacg tttaaactaa ctggctttac ggatcatgcc
      541 aaattcaatg tctcaccagt tctctataca accatcaatg cttaccaaga gatccgcttc
      601 gaaaaagaag atacaagtga aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc
      661 agtaatttac ctgaagattt agaacagatc aaaatttcta aattcatcaa tgaattacct
      721 ggatataatg cgcaagtatt gacttttgga tttatgatcg gatttttgat cgttattgcg
      781 gctattgtga tcgggatttt catttatgta ctgacgatgc aaaagattaa tatctttggt
      841 gtaatgaaag cacaagggat cacaggagga tttattgcgc gttctgtagt ggcacaaaca
      901 ttcattttat catttgttgg tattcttctt ggtctattag gaacagtggg aacatccctt
      961 gttctacctg atgcggttcc attccaaagc aattggctgt tttttggcgt cattagcttg
     1021 ttgatgctgg tagtagctgt gttaggcgca ctattttctg ttcggacaat cgtaaaaatc
     1081 gatccgttga aggcaattgg ataagaagga ggaagagcat gaaagcaata gaatttaaag
     1141 aagtagaaaa aagtttccaa gatggcgacc aagtagttaa agcattgaaa gaaacaacca
     1201 tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta
     1261 cttttttgac tttggcaggc ggattgcaga cgcctagcag tggctctgta tttatcaatg
     1321 atcaagaatt cagtaatcaa aaagaaagtg aacgcgttaa actacgtttt aaagaaattg
     1381 gctttatttt gcaagcttcc aatctggttc cttttctgac agtaaaaaag cagttattat
     1441 tagtagataa gatcaaacat gaacatcgtc aaaaagaagc aaaagaatta tttgagcagc
     1501 ttggcgtgga taagttgatc aacaagtatc cagaagaact atctggtgga gaacggcaaa
     1561 gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat tattctagca gatgaaccaa
     1621 ctgctagttt ggattcagaa aaggcacgag aagtagtcga tatcttggca aaagaatcaa
     1681 aagacaaaaa taaagcaacg atcatggtta ctcacgatac acggctgatt gacaaatgcg
     1741 atagaatatt tatgatggaa gatggtgtat tgaaagaaaa agaataacca aaaagcaggt
     1801 ggaaaaatgg tccatcgata caacaaagat acacagcaaa aaattttgaa agaagcgact
     1861 gacttgttta tgtccaaggg atatctcggc acttcaacaa gagagatcgc acaaaaagtt
     1921 gggatcaccc aacccaatct ctatcattat ttcggtgata aagaaaaact atatacggca
     1981 gttttagaaa accacttaaa agatgttgga aaagctttga gagaaatcgt tcaaacaagc
     2041 gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt ttttgattga gacacatctg
     2101 gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc tttcaaagga aacaagagat
     2161 catcttttct ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat cttttcagca
     2221 aaccaatctg tgctaagaaa tgggatttca aaagaaatcg cagcaaggca ttttttctta
     2281 gtgttagcac cttacatcac tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg
     2341 caattgatcg atttatattt gaatggtgta ttaggagaag cgtaaagctg ttatataggg
     2401 agaaaaatag ttaggtgaag aagcaagata tgacgaagat cgttatatct tgtttttttt
     2461 atataggtag tttttcataa atacaaattt gttattctct catgattatc agggtatatt
     2521 tttatttatt atgtcggtgt tccaggaagt gttttaattt aataacgaag aaattaattg
     2581 tttgtagaat gaaggaaact atttattggg tagatgaatc accacgaata gtcaaaagga
     2641 gatttcagga tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta ttttctacgg
     2701 ttatatcttt tacagcccct tttttgcgta cttaagctga ttttaagttt tgacccttag
     2761 tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca
     2821 atcattatca ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga
     2881 caaacggtca gcagtacgaa cagtacggtt acaaagacaa aggaatcatt tgatgataca
     2941 gaacaagaga cattaatcaa aatcggaaaa aatgaccgta cactatatac agatgactcg
     3001 ctaactaccc cacttgcaac tataaacggc ggagagctgg cggagtttct ttctgaaaca
     3061 aaaaatgctt atcagatcaa gacaaatgat ggctatactg gatatttagc cctcgaagat
     3121 ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag agctatctga tgcagtgatt
     3181 gtattagatc ctgggcatgg cggaaatgat actggggcat taagcaatga tgaacaaaca
     3241 gaagaaaaag atataacatt aagtacagcc ataaaagtaa aaaaggcgct agaggatgct
     3301 ggagcaacgg tttatttgac ccatactaca gatgaattag tacagcttgg agatatatgc
     3361 gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga ctctacggaa
     3421 tatgcgaatg aagcaactgg aatcacgaca tattattact atggacaaga ggaaacatta
     3481 gcacagacaa ttgctgactc cttcactgat ctgcctctgg attcaagagg aatctccact
     3541 gggaactatc aagttcttcg agaaaatctt caaccttcta ttttaatcga gatgggttat
     3601 atgaataatg atagtgattt aaaggaatta gtaacagaca gttatcagca gcagattgct
     3661 gcaagtttga cacaagcatt gaccacttat tttcagtaat aaataagccc agccaaacaa
     3721 aaatagctcc tcatattttt cgtttggccg ggcttattat cgcagatgca agcttttgaa
     3781 caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc cgcgtcacaa ggagcaattt
     3841 caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcatga
     3901 gaacaccgtt tattcggaac aagggactgt gacatgactt ttgtcacagt tccattattt
     3961 ttttagcagc ttcaaagctt tttctgtatt ggcaaaatga agtttggcat atttttcaag
     4021 cataggcatg ccgcctttct ccaatagttt tgccgctact tggtcagatt tcgttccagc
     4081 tcctgatgga agggtgattc cagcttcagc tgatgctttg tgtaactctt ccaaaaagct
     4141 ggcacgactg atgatagaag cagcagcaac agctaaatga tattgttctc ctttagtaac
     4201 gaagtacagt ttttcttgta attgattttt ttctaaacgc gcatattttt taaaatttgc
     4261 ttccggcgta aactgatcga tcaaaatcgc ttctggtttt tctggctcga ttttttttag
     4321 cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc cgaacggcat tgtattttgg
     4381 ttggatctca ttgtattttt ctggagataa gacaagcagt ttatagggga tggttttttt
     4441 taagatgtca gcaagctgga tgatttggct atcttttaac tcttttgaat ctctaacacc
     4501 aagtttacgt aattctgcaa gctgatcacg tttcacataa gcagcacata ctgttagcgg
     4561 accaaaatag ctgccatttc cgacctcatc actacctaaa acggataatt gactgatatt
     4621 ttttggcaaa gaagaggctg tttttgctgg cttggcttgt ttatcctgtg cctcccccca
     4681 tttatcagct tctattgctg cattcgtacc ttgaaacatc acttttccgg atgtataagc
     4741 ggtaatggtc gtcccgtctt tttttgcagc aaagctagta taaggaatct gtttatttaa
     4801 caaataagag cgatagtatg ctttcatttg actaagttgg tcgtttgttg ctttgataat
     4861 gacgttgctc ataaaattct cctcgttttt atacataaaa ctataccgca actcacgact
     4921 tttcgttttc taagagattg cgagacatag cattttttta aagtttatca tataatagtt
     4981 aagtatgtga aaaatatttg tggagaacgg tgaaagaacc aacttcatgg tacactaaat
     5041 aaaactttca tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa
     5101 gcagtgattg ccaaccaaac atatactatt atcggacggg aaaccaagca tcatatggat
     5161 atcgtaacga agctgatcaa tgaacagtta gcagaattga aacaattatc accacaaatg
     5221 gataatgaac aagcagcaat cttgatggct gtcaatgcgc tttcggatca attgaagaaa
     5281 caagaaagaa tcttggagct ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa
     5341 tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa
     5401 gtattgaaag aaaatggtca agcagatcat accattaaaa atcatgtgga agcacaacag
     5461 attttaaacg agaaacgaaa aggacaaatt aaacaaaaat cttctaatta agatcctttt
     5521 atcaaaataa gaaaaattta aaaaagttag aagagaaagg aacgtagcat gctgagtttg
     5581 ctgattttat ttttattgct aattgcgttt ttttctggag cacgtcgcgg gtttgctttg
     5641 caaatcatat tcgcgattgg atatgtattg tcatttattg ctgcccagca tttttacaaa
     5701 ccattagcaa gtcatttgga attatacatt ccatacccag ctgtcacccc aacatcaaag
     5761 ttagcatttt ttgatcaggt atttgctttt cacctagatg aagcttttta tgcaggaact
     5821 gcttttctga ttattttgct aattggctgg ctgcttaccc gatttgtggg ggtttttgtc
     5881 catggattga cgtatgtgcc gatcttgaga caggtagact ggattgcagg aggcatcctg
     5941 tcactaatca tggcttacgt tactatattt ttgattttgc agttacttgc ctttgttcca
     6001 ttagtgtggt ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc gttgagagaa
     6061 cgccatttct taccaacaag atgtatgagc tttgggtaac gaatattttg aattaaggaa
     6121 aatagtgctg gaaaatattt cgttttttgt acagagtagt ttatttaaga aggctgcgta
     6181 gcgtatgtcc ggcacagctc ttttttcata gtgaaagata aggtgataaa atgaataacc
     6241 gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa tttgtagtga
     6301 ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa caaacgatca
     6361 caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga ggcgggatcc
     6421 ctatacctaa acttgaaaat atccgtcccc atatgaaacg gatcgagatc ggagcagact
     6481 tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc gaattgaatc
     6541 gtttcattga tgatctttct gacagtgaaa tcgaatttgc acgtttgtat atgtgggcag
     6601 atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc gatgaagacg
     6661 gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac attcggcgaa
     6721 gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat gcaaagtatt
     6781 taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc aaacaagaat
     6841 accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggacag actttattta
     6901 ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa atcgctgaac
     6961 gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc catcgtcaag
     7021 aaatcatcca caatgcttat gtgattggta aaatggatct gatgaatgca aaagcacgat
     7081 ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga agacaatcat gtcgtcttga
     7141 aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac atcacaatcg
     7201 gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa acgatcacgt
     7261 tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt cctgcaggtg
     7321 aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac gaacagtcga
     7381 ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat attttatcaa
     7441 aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca gatccgcaag
     7501 aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct gcttatgtga
     7561 tggcaacaac gcattatcct gaattgaaag tttatggata caatcgggca aatacgatta
     7621 atgccagcat ggagttcgat gtggatacac taagtccaac gtatcgtttg ctgattggcg
     7681 ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat acagaagtta
     7741 ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa atgatcacag
     7801 atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt cattttgttt
     7861 ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc tttgaagaac
     7921 gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca gaggcggaag
     7981 aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt gggcaaggaa
     8041 atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta catcaagaag
     8101 aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg ttgaaaccgg
     8161 gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt aaaaacggga
     8221 atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac gagctgacgc
     8281 ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga tctgaatcta
     8341 gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa gcgctaagtg
     8401 aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg actattgtcc
     8461 acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa aaccatcgga
     8521 gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg acaattgtta
     8581 aatttaagta aagcttacta aaggtaagca gatggttcgc tatccaagta tagaaatgct
     8641 atactaatca tagattttaa aaatggaggt ctttgatatg tcacaagtaa ttacagataa
     8701 gacatttaat gaagaaacag ataaaggact tgtattgatt gatttttggg caacttggtg
     8761 tggtccatgt cgtatgcaag caccgatttt ggatcagttg gaacaagaat atgatgaaga
     8821 agaattcaga attgctaaaa tggatgtgga tgaaaatcca gaaactcctc aacaattcgg
     8881 tatcatgagc attccaacat taatgttgaa aaaagatggt caagtagtag aaaaagctgt
     8941 tggtgttcat tcaaaagaac aactacgtca aatgatcgat caatatttat aaaaacttac
     9001 agcaaaaata agctgagggt gggactgaat ccagttccgt cctcggtttt tttgcggttg
     9061 agttaaggtt attcaaatta atattttttg agtataatta cgttgtcagg ctggattgtt
     9121 tttttgacag acatgctata ataactttaa ttgaatatga aggggagtac cagatgaacg
     9181 aacgaatcaa aaacaaatta gctttacttc ctgatcagcc cggctgttac ttgatgaaag
     9241 ataaaaatgg aacgatcatt tatgtcggca aagccaaaat attaaaaaat cgtgtccgct
     9301 catattttac tggcagccat aatacgaaaa cggaacggct tgttagtgaa atcgaagact
     9361 ttgaatacat cgttacagaa tcgaatatcg aagcattgct cttagagatc aatctgataa
     9421 agaaaaatga tcctaaatac aacattatgt taaaggatga taaaacctat cctttcctta
     9481 agataacgaa tgaaaaatat ccccgtttgg tgattactcg taaagtacta aaagacaaag
     9541 cactgtattt tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag aagatcctcg
     9601 atcgattatt tcctctgaga aaatgcaaac ctagtcagac gaaggagcct tgtctttact
     9661 atcatttggg acaatgtctg tgtccttatt atttcgacgt agatccgaaa gtctatacga
     9721 aaatcgttga tgaagtgaaa aagtttttaa acggaggaca cgaaaaaatc gagtcagaga
     9781 tccatcagaa aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct gcagaatacc
     9841 gagaccagat ccgtgccatt gagacaatca tgactcgcca aaaaatgaca aatactgatt
     9901 tactggatcg agatgtattt ggttatgcga ttgacaaagg atggatgtgt gtccaagtgt
     9961 ttttcgtaag acaaggaaag ctaattgaac gagatgtttc aatgtttcct ttctataacg
    10021 aagcagaaga agatttcttg acttacctcg gtcagtttta tcaggaaaat gagcatttca
    10081 ttcctaaaga agtcttgatt cctgatacga tcgacaagcc cagtgtagaa gctttgttag
    10141 ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa attagttgaa ttggcgaata
    10201 agaatgcctc agttgctttg aatgaacggt ttgatctgat tgccagaaaa caggaacgga
    10261 cgatcggagc agttgaacgt ttaggaaacg cgatgaatat cccgacccca atacgtatcg
    10321 agtcttttga taactctaat atcatgggga cgaatcctgt ggctgcgatg gtcgtattca
    10381 ttgatggaaa accggcaaga aaagaatatc ggaaatataa aataaaaaca gtcaacggac
    10441 cggatgatta tgcatcaatg agagaagtca tctatcgcag atattcacga gtgattcgcg
    10501 aagggttgcc tttacctgat ttgatcttga tcgatggagg aaaaggacaa gtagatgtgg
    10561 caaaagatgt cttagaaaat caattaggaa tcgatattcc tattgctggc atggcaaaaa
    10621 acgataaaca taaaacaaat gagttgctat tcggtataga tttggctgta gtaccgcttg
    10681 agcgaaattc accagagttt ttcttactcc agcgaatcca agatgaagtc catcgattcg
    10741 caatcacttt ccatcggcag ctacgcagta aaaacagttt tgcttcccga ttagatgaaa
    10801 ttgaaaggtt aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa tcgttgaaaa
    10861 atatcacagc agccacgaca gaagaattac aagaaatcgg actgccgaaa aatgtcgcac
    10921 aaaacgtatt tgataaatta catcaggaat gaggaaaggg cactttacaa agaaagtaaa
    10981 gtgtcctatc attttatgta caacaaaaag agcgtttacg ttttgttggt aaataataaa
    11041 tcgcttacgt ctggtgtcta aggatatatg tgatttcttg aagaaggagt gaagaaatgt
    11101 tagaagtcaa ggatcttgtt aaaacttttg ggaactacac agctgtcgat catgtctctt
    11161 tccaaatccc cgatggaaaa atcatggggc taattggcca aaacggggct gggaagacaa
    11221 cgacttttcg tttgatcttg gattttttga cacaagacca aggagaagtg ttatggaatg
    11281 gccatcatct tggggagaaa gattatgatg tcatcggtta tcttccggag gaacggggac
    11341 tttatccgaa agtaacgatt caagaccaac tgctttattt tgcagaactt agagggaaat
    11401 caagaaaaga gattgaacca aaaatcgatt tttggatgga aaaatttcag gtaaaaggga
    11461 aaaagacgga taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc cagctgatcg
    11521 ccacgttaat ccatgaacct aaattgatca ttttggatga gccattcagc gggttagatc
    11581 cggtaaatgc tgaattgcta aaagatggaa tattggaatt gaaaaaacat ggttcatgtg
    11641 tcattttttc tagccacaat atggataatg tagaaaaaat ctgcgatcat ttgatcatgt
    11701 tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa tcatttggcc
    11761 gtacgaagtt attcttggaa tctggtttaa gccaacagga agtagaagaa atcgaaggcg
    11821 tgaaaaaagt gatccttcat gaagacaata cgttagagat taccttagaa gatcctgaag
    11881 ctgggaaaga aatctttact cgtgcgactc agttcggcta cattcctatg tttaaccagc
    11941 aaccgccaac tttggaagag attttcaaat tgaaagcagg tgaaatcgat gcgtaagttt
    12001 tggattatta ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt cttgatcatg
    12061 atattagtcc cttttatcat gctgggtatc atttatgtag caggaaactt tgctcaacaa
    12121 aatagtgatg tcaataaaat cggcgtattg gctgataatg agcaaatcag tcagcaaatg
    12181 agccaagtca aaacagatga ttttgagttc aaagcagttg attctgaaga aaaagcacaa
    12241 aaagagcttt cggaagaaaa aatcgatgcg tatttagtag tctcaactga gaatgataag
    12301 ataaatggga tgctttatag cgaaaattca ttaggacaaa gtacacaact actgatacaa
    12361 caacaactga ctggcttgca atcgatgctt cgagcaagtc aattaggcat ttcaccagaa
    12421 gaagtagcta gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa ttttgattca
    12481 aaaggaaaaa tgacagttgg agaagataac tcgacagtcc aatatgtcgt aagttatgta
    12541 gcaacgattg tcttgttcat tattattttg acgtatgcgc agatcattgc acaagagatc
    12601 gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac tgcacaaaag
    12661 catttttacg gaaaactaac aggtgtttta cttgttgcat taacgcaaat ggcattatac
    12721 ggtattattt ttggtatagg atacagtcaa ttcaaagata tggaaatggt caaaaatata
    12781 ttagacggta tctcattgga taacttgatt ggaccattct tgtggtattc tcttctattc
    12841 atgatctttg gcattttgat tttttctgtt ttagcagctt tatgtggctc tttggtcaac
    12901 aaagcagaag atacagcaaa agcaattttg ccagtgactt atctatctct aggcggatat
    12961 atgcttggat tgatactagg agcttcagat ccaaacaata tcgttattcg gatcacttcg
    13021 tatattccat tcttatcttc atatattatg ccggttcgct tagcaaatga gacagtcggt
    13081 atgggtggag cattgatttc cttgttggta cttgtagccg taacattctt attgatgatc
    13141 ttgtctgcaa acatgtacaa atcgaatgta ctcgtgtata gcgaaggcgg attatggagt
    13201 tcattgaaac aatcgatttc aattatgaga aatgaacgaa aaaaagcata aaatatagaa
    13261 aaatatagaa ggaagctgga catttattgt tcccagcttc ttttttgtat agacctattt
    13321 ctattttttt attatattgg atatatatat tcattgaaaa tagagcataa caactttata
    13381 atgttgagta taccaatttt tggaggcgtt ttaatggacc gctatttaga tttactttca
    13441 gagaagttcc ctacaacgga agatgtcatc acggaaatca tcaatctcga agcaattttg
    13501 cagcttccaa aaggaacaga gttatttttg agcgatatac atggcgaatt tcctgctttt
    13561 gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga ccttttctct
    13621 gaacaaatga cggaaacgga gatcagccaa ttcactattt ttacggctta tccagaatat
    13681 gtgttgacga ctgattggta caaaaaacaa gataaatccc agctgatcca tcgtttgatc
    13741 gatcttttac ggtttacgac agtcaaatac actcgatcaa aagttcgaaa aggattgccg
    13801 aaagaatata gctatatcat tgaggaactc ttgtatatcg atgatcgtat caacgggaaa
    13861 aaagactatg taaaaaaaat cattgaacaa cttgtcttga tgaaggaaga agaaaaattt
    13921 ttaacaaaac ttgcgcagac gatccaaaaa tctgtcatcg atcatttgca tatcgtgggt
    13981 gatatttttg atcgaggaac tcaagcagcc aaagtaatgg atcgaatcat ggatttttcc
    14041 tctgtagata tccagtgggg gaatcatgat attttgtgga ttggtgcgta ttgcggctct
    14101 gaggcatgtt tattgacgct tttacggatt gccgcacgct acaactattt atttgaatta
    14161 gagaaagaat atggattaaa tttacgtcca ttattttctt ttgcccatca aacctatcag
    14221 aagaatccag tatttacccc taaaaacagg aaagatctat ctgaaagaga gcaagaagaa
    14281 ttagaaaaga tccatcaagc actttctatc ctccaattca aatcagaaca tcagttgttg
    14341 aatcgacgac ctgaatttaa aatggacgat cgtaaattgt tggaaaaaat caactatgag
    14401 gaatcaacga tcgttttaga aggtcagctc tatgagttga aagggacttg ttttcaaacg
    14461 atccagccag atgatcctaa aaaattcttg gaagaggaac aaaaagtgat cgatagtttg
    14521 atgtattcat tccagcactc gttaagaatg caaaaacaca tgacgttttt gatcgaaaaa
    14581 gggggaatgt atctcacgaa caatcagcat gtactgttcc atggctgtat acctgtagat
    14641 gaaaaggggc agctcttagc atttgaatta gaccaatctt atcgcggaaa agaactgctg
    14701 ggattttttg aaaaacagat aactgaaagc gcaaaagatt tgactgcaaa tgaagatttg
    14761 gcgaccgatt tgatttggta tgcgtggagc gggccatttt cgcctttatt cggcaagagt
    14821 aaaatggcaa cttttgagcg ctattttctc acagagcctc atacgcacgt cgaaaaaagc
    14881 aatccctatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt ggcagaattc
    14941 gatgcaaaag aagaaggttc agcaatcatc aatggacaca cgccagtaaa agtaaaaaaa
    15001 ggagagtcac cgatcaaagc aaacggaaaa gcatttgtta ttgacggagg tctatccaaa
    15061 gcttatcaaa aaacaaccgg gattgctggc tactcattac tatgcaattc ttacggtttc
    15121 caaatcgtga cacattatcc gttcttgcct attgaacaac aatttgcaaa aaaatctgat
    15181 caaacgaatg taaaaaaagt gatcgagcag cagctcccac gcaaacttaa tcggaatacg
    15241 acaaaaggaa tggaattaca gcaacagatt actcaactcc aaaggctgtt ggattatcga
    15301 aaggacgatt gagaaaaatg ctagccaaca ttgctcaata gactaaaaag cgactgcaac
    15361 aaaaggcgtg tcgcagtctc cttcggaatt agctcaaaaa acggtaaaat attagaagct
    15421 atttttctgt ttatttctct aatactcagg tcagggcgct gatttcacga cctcttcctt
    15481 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaaca aacatgagaa
    15541 gctgtttttt gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctaatc
    15601 agaaaaacga ttaaaattca ttcacaaaga aatcgaataa acgctgttca gatgaatcta
    15661 cacgatgaga aagttccgga tgccattgga caccaaggat tttgtgcata ttatctttac
    15721 tttggactgc ttcaatgagt ccatcgttgg aaaaggcgat ggcttttaac aaaggagaaa
    15781 gatctttgac tgcctgatga tgataggaat tcacaaagta tgaatcaggg agaaggtcac
    15841 ttaataaact gtccgaaacg attttgactt cgtgagttgc aaactgcggg gcagtgggct
    15901 gttgttcgtg ttttacggac cattttggat ataatgaaag atcttgataa agtgttccct
    15961 ccaacacgac attcaatagt tgaagtcctc ggcagactgc aaaaatcggt tttctttgtt
    16021 tcaatgcttc caagatcaat gcctgttcga attggtctcg ttgaatattt gtttccgtca
    16081 gttttggatg agggtcttcc atgtaaagtt gcggactaac atcctgaccg cctgcaagaa
    16141 tgagtttgtc gattttctca attagttgag gcgctgaatc tggagacatg actggcagaa
    16201 taagaggtaa tccttgagca tctaaaactg catccacaaa tccctgcggt gtatagctta
    16261 cttgatttcc ttggaaagta tctgtcgctc gaatcagttg attcccggca atgccgatga
    16321 ttggtttcat ttttattccc cctaaagtag actaattgta acaaggattt aaaaaaattt
    16381 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    16441 gaattttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    16501 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    16561 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    16621 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    16681 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gagaagcgtg ctcgaaccct
    16741 acggcagcag ttgaagacct atgcagaaga aaaagagcaa gaattggaga taatccaaac
    16801 gaacagtcgg caacagctga aacagttggt agaagaaaaa gaacaattcg aaaagcaaga
    16861 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    16921 agaccaatat caaaagcatc agagtcaagc aatcggtgca atcattgaaa gggtgaaaga
    16981 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    17041 agaggatttt acaagcagta caaggaatcc aaaatgttga aatccgggat ctgtatcaaa
    17101 atagaacaaa caaccagtgg gtagaaaaat attttccaga taccacgatt attgataaag
    17161 aagcaaaatt aagcgcttta ggcaatcggc tgaaagacat ccaagaagct atccaattca
    17221 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    17281 tacaagactt ggaagcaaat tattccgaag aaaatttcat gaataaatta gaagaagtcc
    17341 ttgatttaaa agaaaagtgg gaacagctaa ctgaaaaacg tgatcgttta gaagaagagg
    17401 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    17461 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    17521 taagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    17581 tttcgtatgt tgctttaaaa aaatcagttg ctgctataga agaaattgct gggaaatacg
    17641 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaattg caagaaacca
    17701 aacagcaatt atccattgtt cacgagcagc aaaaaaagat aacgacagcg ttaggtcagt
    17761 gcagcggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    17821 gggaaaaagt aaaagaacgg tttatccatg ccacttatct gatcgttttg caaggttggg
    17881 tagatgcaga agaaaaacag aatttgatgc atgtgttaaa cgaaactgta tcagaagaag
    17941 atatatacgt tagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    18001 aattgaaaaa ccatccgctt atcgaacctt ttgaattgct gactgaaatg tacagtctgc
    18061 caaaatatga agaagtagat ccaacgcctt ggatgacacc attttatctt gtcttcttcg
    18121 ggatgatggt cgcagatgtt ggttacggat tgctgatgct cattgggaca atactagctc
    18181 aaaaactatt tgttttaccc cgcgggatga aacgtttcgt aaaattcttt gagattctct
    18241 cggtaccatc gatcatctgg ggattgatct acagttcatt tttcggacag gcattaccaa
    18301 aagaactatt tggtatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    18361 cgattttgat tttatccgtg attttcggtc tgatacagat cttggttgga ttattcgtgg
    18421 cagcaaagga aaatatcagg aggaaagatt acttgagtgc tgtcagtgat ggttttgcct
    18481 ggcaagggat cctgattggg atcgttattg cattgattgg tgtcatgttg ataaaaaata
    18541 cagcatttgt ttatcttggc gcggggctat cgatcattgc tgctttatgt atcttaatcg
    18601 tgccgattat ccaatcgccg tctaaagtca agggtgctgc caaaggagcg tataatcttt
    18661 acggattgac tggttatatc ggtgatttag tcagttatac acgtttgatg gcactaggta
    18721 tttcaggcgg aagtattggc gcggcattca atatgctagt tgctttcatg ccgccagccg
    18781 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    18841 cactgttaag tgcctatgtc catggtgcac gattgcagta tgtcgaattt tttggaaaat
    18901 tttatacagg aggcggacgt gcctttgacc cgctgaaaac ggcggaaaaa tacgtcaaca
    18961 tcaatcataa gaaaaaaaac aataaataat tggaggattt ttatacaatg atggattact
    19021 taattagtca aaatggtgga atggtatttg cagtattagc gatggcaaca gcgacgattt
    19081 tttcagggat tggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcgttaa
    19141 caacaagtca acccgaaaaa ttcggacagg ctttgattct gcaattgctt ccaggtacgc
    19201 aagggttgta tggattcgtt atcgcctttt tgatttttat caacttagga aatgatatgt
    19261 cagttgtaca aggattgaac tttctaggcg cttctctgcc gattgctttt acaggtttgt
    19321 tttcaggtat cgcccaaggg aaagttgcag cagcaggtat ccaaatttta gcgaaaaaac
    19381 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatat gcgattttag
    19441 gtttcgttat ttccttccta ttagtattga atgcctaagc ataaaggagg aaaagcagat
    19501 gaacgccatt gaaaaaatca tctctcagat gaatgaagca gcagagcaag aaagagcggc
    19561 gttagagcaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaaat
    19621 cgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttacttgaaa aaaaataccg
    19681 tcagcttagg aatcgccaac aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    19741 atttttacga cgattgtttg cagatgcaat agctgaaatg gagaattggg atgaatctga
    19801 acaaatccag tttatcaaaa attcattgta ttcattacct ttgacaggca aagtaacatt
    19861 cattgctgga gaaaaatcag cagcttatct ttctcagaca ttgctggatg aatggaacaa
    19921 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    19981 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    20041 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    20101 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    20161 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    20221 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    20281 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    20341 tggatctata cgatgcgcta tactttccat aatctgaagg ttttgacaaa agccgagatg
    20401 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    20461 aaggatgcca ttcatacgca agtttcaaat gaactccctg aaagcattat ggacagtata
    20521 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    20581 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    20641 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    20701 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    20761 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    20821 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatct tgatctcgta
    20881 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    20941 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    21001 cttcgtttac tggttatcgg caaaagaagc catttcgaac cagaaacaat cagaaagcgg
    21061 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    21121 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    21181 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    21241 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    21301 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    21361 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    21421 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    21481 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    21541 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    21601 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    21661 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    21721 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    21781 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    21841 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    21901 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    21961 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    22021 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    22081 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    22141 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    22201 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    22261 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    22321 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    22381 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    22441 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    22501 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    22561 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    22621 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    22681 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    22741 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    22801 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    22861 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    22921 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    22981 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    23041 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    23101 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    23161 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    23221 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    23281 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    23341 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    23401 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    23461 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    23521 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    23581 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    23641 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    23701 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    23761 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    23821 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    23881 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    23941 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    24001 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    24061 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    24121 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    24181 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    24241 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    24301 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    24361 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    24421 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    24481 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    24541 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    24601 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    24661 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    24721 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    24781 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    24841 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    24901 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    24961 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    25021 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    25081 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    25141 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    25201 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    25261 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    25321 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    25381 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    25441 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    25501 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    25561 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    25621 ttacatccta aaattggcct ttgttatcca attgattggt acggttgctt tatcttttgt
    25681 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    25741 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    25801 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    25861 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    25921 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    25981 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    26041 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    26101 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    26161 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    26221 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    26281 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    26341 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    26401 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    26461 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    26521 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    26581 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    26641 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    26701 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    26761 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    26821 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    26881 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    26941 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    27001 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    27061 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    27121 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    27181 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    27241 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    27301 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    27361 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    27421 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    27481 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    27541 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    27601 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    27661 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    27721 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    27781 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    27841 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    27901 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    27961 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    28021 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    28081 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    28141 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    28201 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    28261 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    28321 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    28381 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    28441 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    28501 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    28561 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    28621 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    28681 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    28741 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    28801 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    28861 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    28921 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    28981 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    29041 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    29101 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    29161 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    29221 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    29281 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    29341 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    29401 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    29461 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    29521 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    29581 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    29641 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    29701 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    29761 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    29821 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    29881 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    29941 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    30001 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    30061 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    30121 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    30181 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    30241 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccggcagctt tatgttgctc
    30301 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    30361 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    30421 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    30481 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    30541 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    30601 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    30661 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    30721 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    30781 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    30841 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    30901 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    30961 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    31021 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    31081 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    31141 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    31201 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    31261 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    31321 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    31381 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    31441 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    31501 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    31561 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    31621 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    31681 gtgccatcac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    31741 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    31801 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    31861 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    31921 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    31981 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    32041 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    32101 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    32161 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    32221 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    32281 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    32341 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    32401 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    32461 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    32521 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    32581 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    32641 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    32701 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    32761 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    32821 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    32881 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    32941 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    33001 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    33061 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    33121 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    33181 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    33241 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    33301 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    33361 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    33421 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    33481 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    33541 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    33601 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccgcgtttt
    33661 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    33721 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    33781 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    33841 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    33901 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    33961 actggagcgt aacctgcatc tttcagtgtt tggatcccgt cacttagaaa aggagttcga
    34021 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    34081 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    34141 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    34201 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    34261 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    34321 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    34381 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    34441 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    34501 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    34561 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    34621 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    34681 aagaccgtcc cacaggaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    34741 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    34801 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    34861 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    34921 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    34981 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    35041 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    35101 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    35161 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    35221 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    35281 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    35341 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    35401 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    35461 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    35521 atcagaataa ggaggtgtga cacgat
//
LOCUS       DAZAOG010000012        32844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000012 DAZAOG010000000
VERSION     DAZAOG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.012"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACHDLD_000554"
                     /pseudo
     CDS             107..1248
                     /locus_tag="ACHDLD_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1374..2453)
                     /locus_tag="ACHDLD_000555"
     CDS             complement(1374..2453)
                     /locus_tag="ACHDLD_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3362312.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2556..2996)
                     /locus_tag="ACHDLD_000556"
     CDS             complement(2556..2996)
                     /locus_tag="ACHDLD_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3362313.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3053..3463)
                     /locus_tag="ACHDLD_000557"
     CDS             complement(3053..3463)
                     /locus_tag="ACHDLD_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3362314.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3643..4077)
                     /locus_tag="ACHDLD_000558"
     CDS             complement(3643..4077)
                     /locus_tag="ACHDLD_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3362315.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4177..5025)
                     /locus_tag="ACHDLD_000559"
     CDS             complement(4177..5025)
                     /locus_tag="ACHDLD_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3362316.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5414..7180
                     /locus_tag="ACHDLD_000560"
     CDS             5414..7180
                     /locus_tag="ACHDLD_000560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN3362317.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7282..8541
                     /locus_tag="ACHDLD_000561"
     CDS             7282..8541
                     /locus_tag="ACHDLD_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3362318.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8688..9989
                     /locus_tag="ACHDLD_000562"
     CDS             8688..9989
                     /locus_tag="ACHDLD_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362319.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9992..10843
                     /locus_tag="ACHDLD_000563"
     CDS             9992..10843
                     /locus_tag="ACHDLD_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN3362320.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10856..11671
                     /locus_tag="ACHDLD_000564"
     CDS             10856..11671
                     /locus_tag="ACHDLD_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN3362321.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11822..12988
                     /locus_tag="ACHDLD_000565"
     CDS             11822..12988
                     /locus_tag="ACHDLD_000565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3362322.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13218..13865
                     /locus_tag="ACHDLD_000566"
     CDS             13218..13865
                     /locus_tag="ACHDLD_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN3362323.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13949..14893
                     /locus_tag="ACHDLD_000567"
     CDS             13949..14893
                     /locus_tag="ACHDLD_000567"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN3362324.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15028..16479
                     /locus_tag="ACHDLD_000568"
     CDS             15028..16479
                     /locus_tag="ACHDLD_000568"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN3362325.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16500..17336
                     /locus_tag="ACHDLD_000569"
     CDS             16500..17336
                     /locus_tag="ACHDLD_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3362326.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17336..17995
                     /locus_tag="ACHDLD_000570"
     CDS             17336..17995
                     /locus_tag="ACHDLD_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3362327.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18121..18810
                     /locus_tag="ACHDLD_000571"
     CDS             18121..18810
                     /locus_tag="ACHDLD_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362328.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18816..19772
                     /locus_tag="ACHDLD_000572"
     CDS             18816..19772
                     /locus_tag="ACHDLD_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN3362329.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19816..20328
                     /locus_tag="ACHDLD_000573"
     CDS             19816..20328
                     /locus_tag="ACHDLD_000573"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3362330.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20584..21132)
                     /locus_tag="ACHDLD_000574"
     CDS             complement(20584..21132)
                     /locus_tag="ACHDLD_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN3362331.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21221..22402)
                     /locus_tag="ACHDLD_000575"
     CDS             complement(21221..22402)
                     /locus_tag="ACHDLD_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN3362332.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22638..24146)
                     /locus_tag="ACHDLD_000576"
     CDS             complement(22638..24146)
                     /locus_tag="ACHDLD_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN3362333.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24305..24496
                     /locus_tag="ACHDLD_000577"
     CDS             24305..24496
                     /locus_tag="ACHDLD_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362334.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24699..25616
                     /locus_tag="ACHDLD_000578"
     CDS             24699..25616
                     /locus_tag="ACHDLD_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN3362335.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25609..26682
                     /locus_tag="ACHDLD_000579"
     CDS             25609..26682
                     /locus_tag="ACHDLD_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362336.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26830..27348
                     /locus_tag="ACHDLD_000580"
     CDS             26830..27348
                     /locus_tag="ACHDLD_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362337.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27329..27721
                     /locus_tag="ACHDLD_000581"
     CDS             27329..27721
                     /locus_tag="ACHDLD_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN3362338.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27797..28732
                     /locus_tag="ACHDLD_000582"
     CDS             27797..28732
                     /locus_tag="ACHDLD_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3362339.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28865..29644
                     /locus_tag="ACHDLD_000583"
     CDS             28865..29644
                     /locus_tag="ACHDLD_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN3362340.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29637..29954
                     /locus_tag="ACHDLD_000584"
     CDS             29637..29954
                     /locus_tag="ACHDLD_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN3362341.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30198..31808
                     /locus_tag="ACHDLD_000585"
     CDS             30198..31808
                     /locus_tag="ACHDLD_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3362342.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31965..32813
                     /locus_tag="ACHDLD_000586"
     CDS             31965..32813
                     /locus_tag="ACHDLD_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3362343.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10853 a         5881 c         6404 g         9706 t
ORIGIN      
        1 tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct
       61 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
      121 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
      181 cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
      241 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
      301 aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta tactacaatc
      361 agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg agaacacttt
      421 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
      481 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatttaac
      541 gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag gaggagatct
      601 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      661 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      721 tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      781 aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
      841 agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa aaccagatgt
      901 gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa
      961 aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat
     1021 aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac
     1081 cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa acagctacca
     1141 aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata
     1201 gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat
     1261 gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc aaaataccag
     1321 attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg
     1381 atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag
     1441 aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca
     1501 agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga
     1561 ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca
     1621 tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca
     1681 cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga
     1741 aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc gacaacaaat
     1801 tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct
     1861 tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc
     1921 ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac tgccattttt
     1981 cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa
     2041 tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc ttttggctga
     2101 tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat aagcatcggg
     2161 atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat catgatagct
     2221 aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa
     2281 taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa gaaaagaaca
     2341 aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa aaaacgctga
     2401 taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt cattatatga
     2461 tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag
     2521 ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga
     2581 aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt
     2641 ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct
     2701 tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata
     2761 taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa
     2821 tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga
     2881 tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct aatgatttct
     2941 ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc ttccattttt
     3001 ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac
     3061 taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt
     3121 tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt
     3181 caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac
     3241 tgacagtaga agccccagag atattttttt gctgtttgat caattctaat gcactgttaa
     3301 aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt
     3361 taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact tctccagagc
     3421 gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta ccacctttaa
     3481 ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg
     3541 gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata
     3601 tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat atttctcaaa
     3661 aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca tccataaata
     3721 accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt ctgtcatttc
     3781 ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga tatcagagac
     3841 gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca aaccaagaga
     3901 cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga cttcattatt
     3961 gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg tttgatacag
     4021 ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt tatgcataaa
     4081 gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc agctaaagaa
     4141 aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat gataagtaaa
     4201 tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg gatcgatttc
     4261 ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca ataaaacctc
     4321 ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac gtccaatctc
     4381 agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca taacagattt
     4441 ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg caatagataa
     4501 aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa caacgaatgc
     4561 gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa aaatgagggg
     4621 agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc
     4681 aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca ttgtttctgg
     4741 aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt
     4801 tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta aaataactat
     4861 agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga cacctccagc
     4921 agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga agagggcaaa
     4981 aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt cacttcctta
     5041 attcattaat aatactctta tcttattata acttactttt caccttaagt taactttaat
     5101 taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa tatacttcta
     5161 cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat
     5221 aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt tttaagtcta
     5281 tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt
     5341 tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat attaaaacac
     5401 aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt
     5461 tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa
     5521 agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca
     5581 acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac
     5641 gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg
     5701 tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt
     5761 ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa
     5821 agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga
     5881 ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact ttttcggggg
     5941 agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca tcaatggcat
     6001 ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga ttgattatta
     6061 tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca
     6121 taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc
     6181 acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat
     6241 ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat
     6301 cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga
     6361 agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg
     6421 atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc
     6481 agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc
     6541 ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag attcgattaa
     6601 agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca
     6661 gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag attctcatga
     6721 tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc
     6781 ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac
     6841 tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct
     6901 agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat
     6961 ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc tctatttcac
     7021 acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt
     7081 agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa
     7141 agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac
     7201 aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg
     7261 aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta
     7321 atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa
     7381 ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat
     7441 ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa
     7501 aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct
     7561 gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg
     7621 gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat
     7681 gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa
     7741 gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt
     7801 cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac
     7861 ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc
     7921 acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca
     7981 acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac
     8041 ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa
     8101 gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat
     8161 aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct
     8221 aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa
     8281 aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag
     8341 gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg
     8401 ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca
     8461 gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa
     8521 gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca
     8581 ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt
     8641 atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata
     8701 aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc
     8761 agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca
     8821 ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta
     8881 taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta
     8941 caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga
     9001 tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg
     9061 atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca
     9121 ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc
     9181 caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa
     9241 gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg
     9301 caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta
     9361 ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta
     9421 ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag
     9481 tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg
     9541 acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata
     9601 tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa
     9661 aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc
     9721 tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat ttctccatga
     9781 tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt tctactgata
     9841 ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt
     9901 cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa
     9961 agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt
    10021 ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat
    10081 catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt
    10141 tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac
    10201 actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca
    10261 agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa
    10321 atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc
    10381 cttttatgtc atggctttct tattagaagc gctcgataag tactggttct tgacaatgat
    10441 ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt
    10501 accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt
    10561 ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat
    10621 gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac
    10681 tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt
    10741 tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa
    10801 gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca
    10861 aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg
    10921 caaactatta tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt
    10981 catgcctgtg actctttttt atgccaatca gatccaagcg attccattag aacaatttct
    11041 atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc
    11101 tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg
    11161 tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga
    11221 acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag
    11281 cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag
    11341 agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct
    11401 tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac
    11461 aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa
    11521 tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat
    11581 cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa
    11641 aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa
    11701 tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt
    11761 agttgattct tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat
    11821 catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga
    11881 agtaaattat tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat
    11941 caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg catctttggc
    12001 actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat
    12061 caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct
    12121 gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga
    12181 cttaggtaac gacttttata aactttggct agatccaact ttgacttatt cttgtgctta
    12241 tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt
    12301 agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac
    12361 attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga
    12421 agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg
    12481 tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt
    12541 aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga
    12601 tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc
    12661 gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga
    12721 attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg
    12781 tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt
    12841 cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc
    12901 agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa
    12961 caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta
    13021 aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt
    13081 gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg gttctttaat
    13141 ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac tattttataa
    13201 caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt
    13261 ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta
    13321 gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta
    13381 gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc
    13441 gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt ttggaattag
    13501 atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg
    13561 aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc
    13621 gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca
    13681 cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt
    13741 gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc
    13801 aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg
    13861 attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca
    13921 aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat
    13981 ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga
    14041 tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg
    14101 aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca attctatggg
    14161 acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca
    14221 ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt
    14281 ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc
    14341 ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac
    14401 actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt
    14461 tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt
    14521 caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt
    14581 catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt
    14641 ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg
    14701 tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca
    14761 gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt
    14821 aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg
    14881 caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa
    14941 cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa
    15001 aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt
    15061 aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag
    15121 caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag
    15181 ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg
    15241 ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac
    15301 cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt
    15361 ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc
    15421 ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct
    15481 gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt
    15541 agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca
    15601 gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt
    15661 tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca
    15721 tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct
    15781 ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa
    15841 aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta
    15901 gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg
    15961 atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt
    16021 tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg
    16081 catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc
    16141 ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg
    16201 ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt
    16261 tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact
    16321 ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc
    16381 caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa
    16441 gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa
    16501 tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg
    16561 gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat
    16621 tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat
    16681 tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct
    16741 cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt
    16801 ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga
    16861 ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact
    16921 cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt
    16981 tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt
    17041 taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa
    17101 tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg
    17161 aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg
    17221 gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc
    17281 aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta
    17341 ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt
    17401 ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg tagggttgct
    17461 tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt
    17521 atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt tcgggttacc
    17581 ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt
    17641 tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg
    17701 tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt
    17761 tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt
    17821 agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac
    17881 attcgattat ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg
    17941 gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa tctgaaaaat
    18001 agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct
    18061 agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac
    18121 atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa
    18181 ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt
    18241 agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca
    18301 aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt
    18361 ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta
    18421 gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg
    18481 aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg
    18541 gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt
    18601 ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct
    18661 aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg
    18721 caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc
    18781 cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa
    18841 aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa
    18901 tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat
    18961 ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat
    19021 tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac
    19081 agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt
    19141 tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt
    19201 cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg
    19261 tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg
    19321 acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt
    19381 tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc
    19441 atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta
    19501 acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat
    19561 ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc
    19621 tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc
    19681 atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa
    19741 aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag
    19801 aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta
    19861 gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac
    19921 gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta
    19981 ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt ggatcccgtt
    20041 tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt
    20101 gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg
    20161 ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat
    20221 gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt
    20281 ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat
    20341 ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg
    20401 ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa
    20461 tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga
    20521 gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta ttattcgata
    20581 catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt
    20641 tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac
    20701 ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga
    20761 ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc
    20821 atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt
    20881 ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc
    20941 aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc
    21001 ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg
    21061 ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata
    21121 gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat
    21181 atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc aagccagtca
    21241 caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg
    21301 cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag
    21361 aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa
    21421 aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag
    21481 tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa
    21541 gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga
    21601 tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg
    21661 ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt
    21721 tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag
    21781 ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga
    21841 tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca
    21901 tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt
    21961 ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg cccatcccgt
    22021 tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc
    22081 gtagaatacc gataatagca gaaataaata caattggcat caatacggaa agaaagaaag
    22141 gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag
    22201 caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac
    22261 gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt
    22321 tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa
    22381 taatacctaa taaataactc aaataatcac tcctattcca aattccgttt tattttacct
    22441 gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta gagtgaaacg
    22501 cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc
    22561 ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg ttttcaacat
    22621 ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag
    22681 gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta
    22741 gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga
    22801 ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa
    22861 gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat
    22921 aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct
    22981 gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt
    23041 gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca
    23101 tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt
    23161 ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg
    23221 aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc
    23281 ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca
    23341 gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc
    23401 acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga
    23461 tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact
    23521 gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt
    23581 tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt
    23641 ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct
    23701 acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct
    23761 agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg
    23821 ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa
    23881 gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc
    23941 agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat
    24001 cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa ttctaaatat
    24061 aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt
    24121 tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc
    24181 atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg
    24241 tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg
    24301 ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt
    24361 taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct
    24421 aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga
    24481 tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat
    24541 ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg
    24601 agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta
    24661 ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg
    24721 caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca ggacaagaat
    24781 cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag
    24841 acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat
    24901 acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc
    24961 aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag
    25021 tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc
    25081 ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag
    25141 agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag
    25201 acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa
    25261 tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg
    25321 aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct
    25381 ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag
    25441 aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac
    25501 gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc
    25561 ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac
    25621 gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact
    25681 tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag
    25741 gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac
    25801 acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat
    25861 caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga
    25921 aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg
    25981 aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa
    26041 ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg
    26101 aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc
    26161 tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta
    26221 tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac
    26281 tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat
    26341 caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat
    26401 acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat
    26461 gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact
    26521 acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat
    26581 caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc
    26641 aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac
    26701 cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt
    26761 cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt
    26821 tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta
    26881 gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc tattttttta
    26941 tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg
    27001 atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact
    27061 gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg
    27121 ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt
    27181 gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt
    27241 gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa
    27301 cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat
    27361 gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa
    27421 ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca
    27481 aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga gtattttgaa
    27541 acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca
    27601 ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg
    27661 tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata
    27721 agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg
    27781 aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc
    27841 tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag
    27901 tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct
    27961 gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg
    28021 acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc
    28081 acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc
    28141 gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt
    28201 ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg
    28261 cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg
    28321 tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga
    28381 tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa
    28441 agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat caaaaatcaa
    28501 gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag ttcatttaag
    28561 tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc
    28621 ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat
    28681 gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta
    28741 ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca
    28801 atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat
    28861 tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact
    28921 ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt
    28981 tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt
    29041 agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt
    29101 tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa
    29161 gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta
    29221 cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt
    29281 ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct cggtgattcg
    29341 agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat
    29401 gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt
    29461 attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt
    29521 tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg
    29581 cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga
    29641 ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct
    29701 tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga
    29761 aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg
    29821 aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg
    29881 cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc
    29941 tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca
    30001 aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg
    30061 gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga
    30121 gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa
    30181 tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg
    30241 ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg
    30301 ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg
    30361 gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg
    30421 tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata
    30481 tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag
    30541 ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc
    30601 catctttgat tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg
    30661 atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct
    30721 tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga
    30781 tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg
    30841 gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa
    30901 gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg
    30961 gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact
    31021 ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca
    31081 atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct
    31141 tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag
    31201 atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag
    31261 ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa
    31321 tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg atgcttcaat
    31381 ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca
    31441 tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta tcaaatggct
    31501 tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag
    31561 cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc
    31621 ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa
    31681 gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt
    31741 ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca
    31801 aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta
    31861 cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag
    31921 ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag
    31981 ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta
    32041 ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg
    32101 atgaatatta tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg
    32161 taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa
    32221 gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg
    32281 acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac
    32341 acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg
    32401 cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat gatttgaaca
    32461 atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa
    32521 tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa
    32581 gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac
    32641 cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt gcggagaaag
    32701 agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca
    32761 ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt
    32821 tattcaaaaa acataccaaa aata
//
LOCUS       DAZAOG010000013        32165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000013 DAZAOG010000000
VERSION     DAZAOG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.013"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(404..763)
                     /locus_tag="ACHDLD_000587"
     CDS             complement(404..763)
                     /locus_tag="ACHDLD_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN3362344.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(793..1134)
                     /locus_tag="ACHDLD_000588"
     CDS             complement(793..1134)
                     /locus_tag="ACHDLD_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362345.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1131..1805)
                     /locus_tag="ACHDLD_000589"
     CDS             complement(1131..1805)
                     /locus_tag="ACHDLD_000589"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3362346.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2981..4279)
                     /gene="asnS"
                     /locus_tag="ACHDLD_000590"
     CDS             complement(2981..4279)
                     /gene="asnS"
                     /locus_tag="ACHDLD_000590"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN3362347.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4319..5509)
                     /locus_tag="ACHDLD_000591"
     CDS             complement(4319..5509)
                     /locus_tag="ACHDLD_000591"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3362348.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5530..6057)
                     /locus_tag="ACHDLD_000592"
     CDS             complement(5530..6057)
                     /locus_tag="ACHDLD_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN3362349.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6104..8893)
                     /locus_tag="ACHDLD_000593"
     CDS             complement(6104..8893)
                     /locus_tag="ACHDLD_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN3362350.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9040..9240)
                     /locus_tag="ACHDLD_000594"
     CDS             complement(9040..9240)
                     /locus_tag="ACHDLD_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3362351.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9693..10013)
                     /locus_tag="ACHDLD_000595"
     CDS             complement(9693..10013)
                     /locus_tag="ACHDLD_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362352.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10516..11634)
                     /locus_tag="ACHDLD_000596"
     CDS             complement(10516..11634)
                     /locus_tag="ACHDLD_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362353.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11867..11989
                     /locus_tag="ACHDLD_000597"
     CDS             11867..11989
                     /locus_tag="ACHDLD_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362354.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12105..13412
                     /locus_tag="ACHDLD_000598"
     CDS             12105..13412
                     /locus_tag="ACHDLD_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN3362355.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13533..14732
                     /locus_tag="ACHDLD_000599"
     CDS             13533..14732
                     /locus_tag="ACHDLD_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN3362356.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14759..16027)
                     /locus_tag="ACHDLD_000600"
     CDS             complement(14759..16027)
                     /locus_tag="ACHDLD_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN3362357.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16083..16149)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16197..16838)
                     /locus_tag="ACHDLD_000601"
     CDS             complement(16197..16838)
                     /locus_tag="ACHDLD_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN3362358.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17247..17750)
                     /locus_tag="ACHDLD_000602"
     CDS             complement(17247..17750)
                     /locus_tag="ACHDLD_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3362359.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17873..18637)
                     /locus_tag="ACHDLD_000603"
     CDS             complement(17873..18637)
                     /locus_tag="ACHDLD_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN3362360.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18745..19020
                     /locus_tag="ACHDLD_000604"
     CDS             18745..19020
                     /locus_tag="ACHDLD_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN3362361.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19580..20530
                     /gene="yidC"
                     /locus_tag="ACHDLD_000605"
     CDS             19580..20530
                     /gene="yidC"
                     /locus_tag="ACHDLD_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN3362362.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20576..20977)
                     /locus_tag="ACHDLD_000606"
     CDS             complement(20576..20977)
                     /locus_tag="ACHDLD_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3362363.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20991..21800)
                     /locus_tag="ACHDLD_000607"
     CDS             complement(20991..21800)
                     /locus_tag="ACHDLD_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3362364.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21787..22677)
                     /locus_tag="ACHDLD_000608"
     CDS             complement(21787..22677)
                     /locus_tag="ACHDLD_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3362365.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22690..23169)
                     /locus_tag="ACHDLD_000609"
                     /pseudo
     CDS             complement(22690..23169)
                     /locus_tag="ACHDLD_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23345..24286)
                     /locus_tag="ACHDLD_000610"
     CDS             complement(23345..24286)
                     /locus_tag="ACHDLD_000610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN3362366.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24283..25278)
                     /gene="lacD"
                     /locus_tag="ACHDLD_000611"
     CDS             complement(24283..25278)
                     /gene="lacD"
                     /locus_tag="ACHDLD_000611"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN3362367.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25271..26467)
                     /locus_tag="ACHDLD_000612"
     CDS             complement(25271..26467)
                     /locus_tag="ACHDLD_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3362368.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26485..27213)
                     /locus_tag="ACHDLD_000613"
     CDS             complement(26485..27213)
                     /locus_tag="ACHDLD_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3362369.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27654..28628)
                     /gene="lacD"
                     /locus_tag="ACHDLD_000614"
     CDS             complement(27654..28628)
                     /gene="lacD"
                     /locus_tag="ACHDLD_000614"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN3362370.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28625..29083)
                     /locus_tag="ACHDLD_000615"
     CDS             complement(28625..29083)
                     /locus_tag="ACHDLD_000615"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN3362371.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29080..30543)
                     /locus_tag="ACHDLD_000616"
     CDS             complement(29080..30543)
                     /locus_tag="ACHDLD_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3362372.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30525..31457)
                     /gene="pfkB"
                     /locus_tag="ACHDLD_000617"
     CDS             complement(30525..31457)
                     /gene="pfkB"
                     /locus_tag="ACHDLD_000617"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN3362373.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31599..>32165
                     /locus_tag="ACHDLD_000618"
     CDS             31599..>32165
                     /locus_tag="ACHDLD_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3362374.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
BASE COUNT         9382 a         6920 c         5511 g        10352 t
ORIGIN      
        1 ttccatacag atgttttggc ttattctttc tttttctctt cgatcattgc ccctgctctt
       61 atccaacccg cacttgcctt cttgatcttg acaggatagc aactaagttt gaagcctgtt
      121 gctggcaatt tgtctaaatt cattaatttt tcgataggac aatatctttt ttcaatccct
      181 gcaaaatgtc cttcccagat attcgaagct ccactacgct ttcaaaaata tagaaagagt
      241 tctttaatga aaattatttc aactttccat aaacagtaat cccatataaa taaaagacgt
      301 aaaagtcaca tgggagaact tatacaggaa aacacataga gtcaggtgaa atacatgtat
      361 cccacctgac tctatgcgtt ttctaacctt tattattctc tttttataca atttctgcac
      421 cgaatggcat caacgccaat cgagcaagtt tgaaagattg cttggcaaaa ggtatcccta
      481 tgatcgtgat acatagaagg atgccgctga cgacatgtgc gagagcaatt ggtaggcctg
      541 aaacgatcag ccagatgata ttaacgatca atgacatgcc agactggtga taaattactt
      601 ctttgccaaa aggcgcaaaa gaaagggagg cgatcttaaa acattgcata ccgattggga
      661 tacctatgat cgtgatacac caaagtatcc ccgctgctaa ccagctaaga ccaccgatta
      721 gtccgccgaa tataaaccag atgacatttc ctaaacaact cattgtattt ctccttctat
      781 tactgttttt gattaaaact gggcaaaaat tgattttatt cttctttttt tagcttccag
      841 catcaagtat tctgcaaatt ttttttggat ttcttccgta ttttcttttg ttactaatgt
      901 gtgttggatt agttttcctg atggtcgctg atccacccgt tcttctgact gttgcacgtc
      961 cgcttgcgca aatccgataa tccgtttttc ttttgttaca gcaacgagca atagtttatt
     1021 cttatcattt aggtcagctg ccgtttttgc ttttacgaat gattctggat gatcagtaat
     1081 cagctgattg atttgaggta aatcttcttt tactgcccac cgtatttcca tcatttctca
     1141 cttttctttc gctcattttt atctccacta aaaaaatcga ctgcggcaat tgcatctatc
     1201 cagctttgtg caatgatctc atgtccgcca atcgttgggt ggaccccatc ttctcttgta
     1261 tatttcgaaa aaccataagc aatccctaaa gcattgaata tgccatctaa aggaaccagc
     1321 aatgcgccat attccttagc aagtttacga acaatttgga ttttagggtc taagtcttta
     1381 cgccagccat gacggtcgtc ggttatagga agtacaaacg gctccatcaa gatgatgttg
     1441 cagatttccg ctgtttgaag ttcatttagt aattttcgat aaacggattc aaattgctgc
     1501 gcagatttct cagttccgaa tatagcctga tcgctagtat tatgccaggt atcattgatt
     1561 ccgatcaaaa tcgatacata gtcaggttgt aaagctaaac aatcttcttg ccagcgtact
     1621 tgcagatctg caattttgtc tcctcctatc ccacggttca tgcattcaaa tttcatcgtt
     1681 ggatattggg agattagttt tccggcaatc aaaagtggat aacctttacc tagatcagtt
     1741 gcttctgctc ggtctcttcc tgcgtctgta atgctgtcac cgataaataa tatacgtttt
     1801 ttcatttgtc aaaaactcct ttttgaataa aacgatgatt ttcttcctta tattatccca
     1861 taagccctat caagacgaaa gagaaaatat atgtataaaa aatgacacca gcctagtccc
     1921 ttcaatcaat tgaatgaacg ctcatcaagt gtctagtttt ttatactttt tttcttaacg
     1981 tgccggattc cccttgattc cttcgttgtt tggtccagag aaatcgatca cacctaagct
     2041 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
     2101 ttccagtaaa tgttctttta ctaatatctc ttctagattg cttgggatag attggtcata
     2161 actttatctg gtccgatgat ttcaaggatg aattaaactt acatatggat cattgcatgc
     2221 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtactaga
     2281 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aataaccttc
     2341 tttgtatatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
     2401 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
     2461 actgcgagag ttcgcagttc ttttttttaa ataaacgttg ctctccggcc aactcctcgg
     2521 tcacaagtca caatagtcat caaacatgaa aaactgtttt ttgaaattac tccttgtgac
     2581 tcggagctga acggcaggcg cacaacctct tgatataaac ggtgttcaat cagctgaccc
     2641 tcgatattag ctaaaaaaac ggtaaaatat gagaagctat ttttccgttt tgtttctcta
     2701 atactcaggt caaggcactg atttcacaac ctcttcctta aacggtgttc aaaagccagt
     2761 ttctcggtat taagccaaat ttatgcaaaa atgtggaaag ccatttccac aaaatttttc
     2821 ttaatactcg aaactaaaca gctttttcac aacctctcca ctaaacggta ttcaaaagct
     2881 tacatctgcg gtaataagtc ccgccaattc aaaaacatga acagctgttt ttgcttggct
     2941 gttctaatta cttgatgcag aacagctttt tcatcacctc ttatggataa attcggttca
     3001 acaaacgtgg gaatggaata gcttcacgta catggtcgat accagcaatc caagtgactg
     3061 ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa
     3121 gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat
     3181 catagtctgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca
     3241 tatctgcaca aattaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga
     3301 tggcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt
     3361 catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta
     3421 attcgattgc atcatcgtaa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga
     3481 cttctttgtc tcgtcctagt acatctaatg catattcgca atgatctaaa acattttgta
     3541 ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca
     3601 tttctggttc gatcatccag aattcgatca aatgacgtcg tgtttttgat ttttcagcac
     3661 ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata
     3721 attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg
     3781 tagtgccttc tggtgcagaa ccagtcaaga tcggcggatc aattttgaca aaatgatgtt
     3841 gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt
     3901 gtcttgaaga acgcagccat aaatgacgat gatccatcaa gaagtctgtt ccgtgttctt
     3961 ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt
     4021 cataaccaaa ttttgaacga ctgtcttcac ggatttctcc agttactaac acggatgttt
     4081 cttggtgtag atttttagct gtttggaaaa cttcttccga cacttcgctt tttacaacta
     4141 caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc
     4201 gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga
     4261 tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt
     4321 aattttgact tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag
     4381 cgcgatcagt ggcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca
     4441 caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatact
     4501 tacacatgcg tagcgtttct tttacattag gaaaaagata gaaagctcct tgcggctttt
     4561 caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa
     4621 aggcgttccg catttcttct actgtatctt gctcaccttt caatgcttca acagctgcat
     4681 attgactgac tgctgtcgga ttgctagttg attgggaagc aacagcgatc attccattga
     4741 tgatcacagg atttcccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag
     4801 acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag
     4861 taaactcatt gccattataa actaaacggc cataaatatc atcagcaact atcaaaatat
     4921 cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa atcattccag
     4981 tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta
     5041 gctgttcaac agtaactttc cagctattgg attcttcccc tttcacaaag acaggaagtc
     5101 cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt
     5161 catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg
     5221 ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga
     5281 ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc
     5341 ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca
     5401 cagtcaaact cagcacatct ctgccttttg cttttaatgc cttggccttt gccgcagctg
     5461 ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc ccattcattc
     5521 ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcaa agccaatcag
     5581 gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc
     5641 atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg
     5701 ttttgcttcg ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga
     5761 tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta
     5821 tgtcttcttt cgagtaaacc aataaaaatg atcggtttcc tcaagatttg catatttctt
     5881 tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa
     5941 aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt
     6001 aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc
     6061 ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat
     6121 ttttctttaa gaattcatgg atatcctgat agatttcctc catagctagc tctttgatcg
     6181 gtactttttt cggtaatgca tggatgatcc gtgcgccata ttttgcagtg actagtcttc
     6241 gatccaaaat aagcaatacg ccacgatctg tctccgtcct tatcaagcga cctaatgctt
     6301 ggcgtaactt caaagctgct ttaggtacgg attcctggta aaaaggattg atcccctctg
     6361 attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac
     6421 gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaagctgt
     6481 cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc
     6541 ttccaccaaa cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt
     6601 tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt
     6661 cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct tgattgctga
     6721 cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat
     6781 aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagcctctta gagagatagt
     6841 gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat
     6901 accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa atcatctgct
     6961 gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt
     7021 gatccatcag caagtattgc ttgtctaatt gatcaaatag gctatataaa cttgctaaat
     7081 cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg
     7141 actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt
     7201 ccagtggcag ctgatccaac atctcttttg tcatcatgac ttcttctgga tactgtccgt
     7261 taggttgctt gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc
     7321 atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg
     7381 gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt
     7441 tatggaaata gttaatactt aatcgtttgc catttacttt ttcagcgatt tccggcaaat
     7501 gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg
     7561 tttcttgtac taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt
     7621 gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa aatccaatac
     7681 ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt aattcatcca
     7741 aatcaccagt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt
     7801 gtttttgttc tggcggttcc acaagtgtgc ctttaaaacg ctgcaaatca atatagtggc
     7861 gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa
     7921 tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt
     7981 tttcaggtgt agccaaataa ctcaatggca gcagataacc gattgtttta cccattcctg
     8041 tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagaccgcat
     8101 tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt ttttcttttg
     8161 cttgtttttt tcttggatat gtccgctcgc cataatggac ggatgtgaat agttccactt
     8221 cttttttctg caaagctagc ccatcgacga tttctaatga gggatcaagt ggctctggtt
     8281 tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc
     8341 tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga
     8401 tcagcaaaag aagttgacct gtcacttcgg catcactatc tgcctgatga ggattctcat
     8461 gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga
     8521 aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc
     8581 gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg
     8641 tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga
     8701 cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat
     8761 gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt
     8821 ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag
     8881 ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact
     8941 acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa acgcccagac
     9001 acgcaaatgt ctgggcgttt attttttagt ataggttaat taagctttag taacgtttgt
     9061 tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt actgcttggc cttcttctaa
     9121 agttttgaat ccgtcgcctt ggatagctga gaaatgaacg aatacatcgt ttccgttttc
     9181 tgcagtgatg aatccaaatc ctttttctga gttaaaccat tttactgtac ctgtttccat
     9241 aataaaaatc ctccttgtgt atcaacacat attttgcaat tacttgaatg gtgtatatct
     9301 ggaagatgtt tatttgaaac aagctaccac agattaccga acaaaaacta ctcctatagt
     9361 atatcatacc gtttacaaaa aagctagagt ttttcgactt tttttattaa aaaaagtttt
     9421 ctcagcgatt acataaagtg aaaaaccgaa ctttttttta aaataaacga catattgttc
     9481 agttggtgtt tttttgctct gatgttcttt tttaaactaa aaaaattatt ctttctgact
     9541 acttatcggt tctgtttcaa cggtcgaggc tggggaagat tctttagtag gtctgatgtt
     9601 ctcatggtat ttctgatagc ctatgacagt taccagaaga acaaggcaaa ccaatacgac
     9661 caacgagatt agtttttctt ttttgctcat atctatacct cataaataac aggatgatgt
     9721 ccatcatatt tctgagtatg agtaaaagca aaagctgcag ctgtagaatg taagaaatgc
     9781 cagcaacttt cataatcaca ggtcggacag taccaaaagt aaccaaatct tggctgcaaa
     9841 gaccgttctt ctgtttcagg aatcgcagaa tcaagatctg gtgtctgtac ataaacattt
     9901 ttgtacagac gtgtgcctgt aataggaaac cgctgtgttt cttcagcaaa aactggctga
     9961 cttgttaaac ttagggatag agacagcaaa caccataaca gtcttttttt cataaagacc
    10021 tccttatcga taagtttttc ccaattaaaa tcatatctca agaacgcccg ttattcacat
    10081 tttgttcata aatgatgttt tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca
    10141 caacctcttt tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca
    10201 caaaaatttg agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct
    10261 gtcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca
    10321 aacaaaaaat atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct
    10381 ttttcacaac ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa
    10441 cggcaaaata tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg
    10501 atttcacaac ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc
    10561 tttttgatcc aaaataaaca cttttccgcc attaatgatc acatcatctg ctaatgcatt
    10621 caacacttga cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt
    10681 tgacgtagcg ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac
    10741 taactgatcg atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc
    10801 gagttcactt gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg
    10861 agaagcagaa atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat
    10921 cgtctgattt tctggtagag caaataaata aatcggccat tgttctggat tttcgaaaga
    10981 atccttcaag tacgtatcaa ctgcttggta atagttgacc caatcgatct ctacttcttc
    11041 atcttttgga tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga
    11101 agagaagttc aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg
    11161 tgccccttct ggtagagata cttcttttac ttgtttgttg ctgacttcat acaatttcat
    11221 ggagtcgcgg ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat
    11281 agccagtaaa taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg
    11341 atggatatag gtttggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca
    11401 aaacgaagga tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc
    11461 ggaatatttt ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg
    11521 atctttttcc acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc
    11581 tttagcgcct tctgaagtca attgtgacaa taattcttta tccttttttg ccataaacgt
    11641 acccctccta aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt
    11701 ccatacaata aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat
    11761 ttttttaagt tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc
    11821 atgaaatgct gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat
    11881 gattttcagt ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga
    11941 tgcggaaaca cagcctattc ctgtaaaagt acgtaaaaac gaccgctaac cttccataac
    12001 gtgcgttctt atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa
    12061 attttaattt gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa
    12121 cacgtctagt cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg
    12181 ttgctgtgca agctgaagat actttcaaag taaatgcaaa agcagccttt gctgtagatg
    12241 cgcaaagcgg taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa
    12301 tcacaaaaat catcggtctt tacatcgtcc ttgatcaagt aaaagaaggg aaactatcat
    12361 gggatgataa agtttcaatt tcagattatg cagaaaattt gagtgtcacc ccagatttat
    12421 caaatgttcc tttgcataaa gaaaacacct acaccgttaa agaattattc gactctgcaa
    12481 tcatccagtc agccaacgct tcaatggttg ccttggcaga aaaaatctct ggcagcgaag
    12541 caaaattcac tgaacgaatg aaagaacaat tgaaagattg gggtatcaaa gatgcaacga
    12601 tcgttaatgc atcaggactg aataactctt atcttggcga aaaccgacca gaaggtactg
    12661 gagaaaacga cgaaaatcaa atgtctgctc aagatgtggc tatcgttgcc cgtcatttga
    12721 tcttggattt tccagaaata ctggatgtat ctagcactac gacacaaatg tttggcgaaa
    12781 acacgcaatc accagtagaa atggtcaact ggaactggat gctcccagga ttcgtgaatt
    12841 ataaagaagg cgtggacgga ttaaaaactg gtacgactga acttgcagga gcctgtttcg
    12901 tcggtacgat cactaaaaac gatcaacgaa tcatcactgt cgtattgaat gcagacggcc
    12961 atgcggaaaa cccaagtgcc cgtttcaatg aaacagctcg cttaatggat tattgctacg
    13021 acaattggtc agaaaaagag cttggaaaag ccaatgcaag tatccctagt ctaaaaacca
    13081 ttgatgtaaa agacggaaaa gaatcaacag tgcccgttgt gcttaaaagt cctgtaaaag
    13141 tatgggtacg gaacgatatg gatactggaa aattaacaat cacaccaact cttgacaaaa
    13201 aacaagtcac tgacaatgag atccaagcac ctatggataa aggaacaaaa atcggtaccg
    13261 caacgatcac cttggcagaa gataaattag gttatcttga agacaacaac gcgccatcca
    13321 cagaaatcat cacggataaa accgtagaaa aagcaaatat tttcgttatt ggctggcgac
    13381 gcgtcgcaga atttttcggt aacttgtttt aattgaacaa aaagaaaaaa gcatctttcc
    13441 atgtggaaga tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc
    13501 gttatactgt ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatttttt
    13561 tggttctcta tcttattggg gtattctcca tcgtttattg gcgaaaacag aaaaaggatc
    13621 cttccttgtt cttttacagc agtacatgga cgtttgcggt aatcttgact tattttgtcg
    13681 tgttggaaat tttttattcg accttttatt ttttgattcc cttagtgttt ttcagtatct
    13741 ttgccttttc ctatttttca gaaaagcgaa ggctgttgaa tggtctttta ttcaatattt
    13801 tcctcattag ttttggtatc tacttgtttg tcttgctata cgaaacacaa aatatttttc
    13861 ttggcggact gattgccttg atcacgatcc cgttactgct tgtctttcta ttcggcattt
    13921 atggattgat cgtttttctt ttttggaacg gtgtcacagt tttacgtaga gagtcgcatt
    13981 cattagctaa tcttttaacg ttgatactag ccatttttct tacattgttt cttgtttttg
    14041 actttttctt gttaaaatat ttaccacaat ggataaatgc tttgtttttc tgcatcccat
    14101 tgatcttgat ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc
    14161 agttcaaccg ccctcgatac aaccaagact tcatcgttgt gttaggcgcc gggttgatca
    14221 atggagaaac agtttctcct cttcttgcca agcgaatcaa taaggcgatt gctttttatt
    14281 gggcacaaag ccgtgcgaca ttaaatccgc ctatcctatt aatgtcgggt ggacagggag
    14341 cagacgaaaa agtaccagaa gcaatggcga tgaaacaata tgccatggaa caaggaattc
    14401 ctgaaagaga tattcttgtc gaaaccaatt cgacaaccac tttggagaat atgcgctatt
    14461 caaaggaaat catggatcag caaatgaaag gtccctatcg cgcgatcttc agttccaaca
    14521 attaccacat tttcagagct ggtctctatg cacgacaagc gaaactgaag gcaaatggca
    14581 ttggggcaaa aacagctttc tattacttgc ctaacgcgtt tttaagagaa tacatcgcca
    14641 tcttgatgat gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggttccg
    14701 cattcttatc actgatcact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc
    14761 acaccttgtt tttttctttt attctcttga cgattggcaa gaatagtatt ccttttttga
    14821 cggctacttg ctgtgcagaa taaataccgc tgtttcctga acacataaag ctgatcaagc
    14881 agatcataaa gaagtaagca gcagcttgtg aaccaaataa ttcgattccc atcacaaaac
    14941 aggcaatcgg tgtatttgtc gcaccagaaa ataccccgat aaaacctaat ccagcaagaa
    15001 aaggaacaga aacatggagc aaaggcgcta gtactgcgcc aagcgttgca ccaatctcaa
    15061 atagtggtgt cacttctccc ccttggtacc ccgcacctaa agacaagacc gtgaagaata
    15121 atttcccaat aaaatcataa gcatgtgcct gtccattaaa tgattcctgc agtaacggaa
    15181 gactcaaccc aaggtaacgt tgtgtttgca aaatcaacgc tgcggctaca actactgctc
    15241 cacctatcgt atttcgccaa acaggatgag gaatccattg cgcataccgc gcttttaaaa
    15301 agacgatcga acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa
    15361 ctttgaagaa cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag
    15421 aaacaccata gctttctgtc atgaaatttg caaaaagcgc agcaaagaag ctaggaaata
    15481 ttgcttctgt tcggacttta ccgattgcca acacttctaa tccaaataat gttccagcta
    15541 aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc actgataacc aaaatctccc
    15601 gttctgcttt atccaatcgg aataaatgcg ctatattatc agctaatgct ccccccattt
    15661 gaactgctgt tccttcacga ccaacagatc ccccaaataa atgggtagtg atggtcccaa
    15721 acaaggtcaa tggaatgagc cgcaaaggga tcttctcttc tcctccattc ccttgatcga
    15781 tcactaagtt attcccacga ctagaaagac cgccatgata cgcataaaga taagcaaaaa
    15841 gcgcgccgct gactggcaat agataaagta gccaaggatg tgctagtcgg atatctgtca
    15901 cgaacgtcag gctatttaag aaaaaagcag acaagctccc catgaatact ccgagaatcg
    15961 ctgtgaccac cagccacttc aacatgtaca tcaaaatacg cactggactc ggtaattgtt
    16021 tttccataaa aaattcctcc tcaaataaaa aaacgcctac taaagaaagg ggctggcccc
    16081 agactttagt aggcgtcatt agctatctta cgttagcatt taaaggcgaa caccatcacc
    16141 tattcacttc cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttat
    16201 ttctttacac gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat
    16261 tgatcactat catgacaaat ataattcccc tttttcgtat acgtgccatc agaaccggct
    16321 ttcgttgtag aaagaaagag cgagacattc gatgttttct gacagatcgc acagattccc
    16381 ttattaatcg ttggtgataa agtaccgctg atccctttta attttccatc ttcgtaataa
    16441 accatgaatt ttttttgcgt ccctggatca ttccaaccga tgtacgtatg tgcttgcaag
    16501 tcgatttcat tccaagcggg ttgtttgagt tttttcactt ttcggaataa tttctcgatt
    16561 tgtggtttgc tcggctgcat aaacggctgg acgatttgct tcagctcctc caatgctcgt
    16621 tctgcttgcg tttttgtcag ttttacatct aaaactgtcg ttaaaaacgt atcgatttct
    16681 gatggttcgt aacctaatac atcgcgaatc ttttcagctg ctagagcctg aaccgtttgt
    16741 accgtttttg agtcattgac agatgagtac gcatttaata actgctctac ttctcttttt
    16801 ataaaaaaat attgatatgg tttcattgtt tgttccatga ttttaaagtc ctttcttcta
    16861 accaaagtgc atactttgta gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga
    16921 tcccggactc ttttaaataa gatacgtttt gcatcttatc atcaccccct ttaatgttca
    16981 ttcttctatg tacatgattc attcgataaa taaaagtaaa ccgtatcttg agcagatagt
    17041 caactgcaaa cagaaaaagg actgtgacaa aagtcgtgtc aaagtccctt tctacaaaca
    17101 aacggtgttc cagaagtagc ttcttcaaaa aataagccgg aatttcacaa aaatttgaga
    17161 agcaattttc gtaaattcct tcttatttct tgaagctgac ccacttctgt cacaacctca
    17221 atatggtcta ttattggttt tacctgttaa atcgaggatt cttttccgaa gtaacgttgc
    17281 catctcttac gcatacttgc tgacataggc tgagcggctt ctttgactgc gcagtatttg
    17341 tctacgaatg ttacgatata tccttctttg tacttaggcg gtgcaatcgt tgcgccccac
    17401 atgtgcttca aaatgatatc tcgctcaaga tccgacaaat cggtgatctt ttctgcattc
    17461 tttacagcga ttcttggatg gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc
    17521 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
    17581 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
    17641 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
    17701 tcttctacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
    17761 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
    17821 ggattattct agtcgccttg gataaaatag ccaaagctta atagttcctt aatcataaag
    17881 aaaaatgata aagaacaatc cctgccgcaa ccccaacatt caacgattcc gcatttcctt
    17941 tcatcgggat atatacgttc tgatcagtca aagacaagat ttcctgactt accccctgac
    18001 cttcgttccc catgattatt gcgaagttat ctgatttttc tactttgtct aaggcgtgtg
    18061 cttcttcatt taattctgtg ccatacactg taaacccttt gtctttcatt aaaggaatca
    18121 cttctgtcaa atctttttgc aacacaggca aatgataatt gcttccctgc atgctgcgca
    18181 gcacctttgt actataaata tctgccgttc ctttgcctaa taaaacacca gccatatcaa
    18241 atgcatctgc cgtacggatc atcgtcccta catttccagg atcttgtaca ttatctaata
    18301 acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt taacgccatc actgctaaga
    18361 ttccttgtgg tgtaggtaga tccgatacag catccatgac ctcatccgaa actagaaaag
    18421 tagaaatctc tttgtttgcg gccagccagt ctccccattc tttcatgcct cgctgtgtca
    18481 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcaatcaga tggaaccctt
    18541 ccaaaagata ttcttccttt tcttcccgat atttcttttt atgtagtttt tttaattctt
    18601 tgattcttgg attttttgct gattggatct ctttcacatt cctcacctcc ccatgattat
    18661 atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttaatttata
    18721 aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc
    18781 caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg cgtgactgga
    18841 acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa tgacgacatc
    18901 atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg agtgcagtat
    18961 gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt ggtgggataa
    19021 ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa atagctctcc
    19081 atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca ccgtcaataa
    19141 ggttgtgaga aagtcgctta gttttgagta gtaaggagaa ttttgtgaaa atggctttcc
    19201 acatttttgc ataaaatttg acttaatacc gagaaactga cttttgaaca ccgtcgataa
    19261 ggttgttttt gagacgcttt gcctcaagta ttaaagatag gtagtaaaaa agctgatcta
    19321 taacttgcaa taaatttgct taacccatat tttccagaca gaacaaaaaa gtttttagtt
    19381 ttttaaataa gaaagaaaca cttggaatca ctcttttctt tccaagtatt tctttcttga
    19441 ttttttgtct ttttttcaaa atttttcgct acgtaaaaga acgaatattt atatgaaatt
    19501 tctatgaagt tgattgaatt ccatgcgctt ttttagtata gttaagtttg tgtaaaaatt
    19561 atagaagagg aagtattcta tgaataaatg gaaaaactgg ttattgggat ccggacttgt
    19621 cagtatcctg ctctttttat ctggctgtgt gcggatggat tcaaacggaa accctgacac
    19681 ttcgggaatc gtttatcgag tactcgtcca tcctatgggc caagcgatta catatctagt
    19741 tgagaatttc aattggtctt atggctgggc agttattgca atgactgtga tcgtccgtat
    19801 catcatcttg ccactaggta ttagtcaatc gaaaaaaaca atgatccaat ctgaaaaaat
    19861 gcaagcgtta aaaccacaag ttgaagcagc ccaacaaaaa ttgaaagctg caacaacacg
    19921 tgaagaacaa atggcagcac aagcagaaat gcagcaagta taccgcgaaa acggattgag
    19981 tatgaccggc ggaatcggct gtctgcctct attgatccaa atgccgatct tctctgcctt
    20041 gtactttacg gcacgttata cagaaggtat tcgggaatca tctttctacg gcatcgattt
    20101 aggctcacca agtatggtac ttgttgtcat tgcaggggtc gcctatctgc ttcaaggata
    20161 catctcaacg atcggtattc ctgaagaaca gaaaaagacg atgcgtacga tgctaatcgt
    20221 ctctcctgct atgatcgtct tcatgtcgat cagtgcgcca gccggtgtta cactttactg
    20281 ggtagtcggt ggtgtgttca gctgtctgca aacattcatc acaaacgtta tgatgaaacc
    20341 acgtttgaaa gcacaagtgg cagaagagtt gaaacaaaac ccgccaaaac aagtcgtcac
    20401 tccaagaaaa gacgtgacag aatcagcaaa gccagctaac aacaaacaag caacaaaaca
    20461 aatcaaacaa ccaaaaaaca gtacaaacaa caaaggccgc aacgcaggaa agcagcagaa
    20521 gcggaaataa aaacatagga caaaaaagat gagagctaag ccctcatctt ttttgttact
    20581 cttcctcttc tgctattcca ttaattaatt cgttgacaga gacaatgctt tcacatcctg
    20641 cacgaacaaa ctcgggctct gaaccaatca tttgtgagtt gataaattca atgaccatcg
    20701 gaagattcat cccagcataa agagtaatct gttgtccttc cattattagt ttagaaatga
    20761 tatttgcagg tgtaccacct aaaagatcgc atagtaccac aaaatcattt aacgaatctg
    20821 tgacagtgag aaacttttgt ttgtatgttt cagtgccatc ttctggtaat aaaggtaacg
    20881 tgtagatatc ctcttgaggc cccatgatca tttctgtact ttttttcaat tcctcacaaa
    20941 attttccgtg actaatcaat accaattgtc tactcatttt tcgctctccc ttatttcgaa
    21001 atcacaccca atgcagataa agcaacacac acaattaata cgataaaaat cgctcgagta
    21061 ggagtcatgt ttttctttcc taataaccaa taaacaccac cgacgatccc agcaggtaaa
    21121 attttaggta agatcatatc gatatagttt tgcatattca atgtcacatc gccaatcgtt
    21181 ggtgcccatg aaaatttaac attgatcatt gtcgcaacta atgcccctac catgaatacc
    21241 cctaataaag tggctgcgtc cgttaatgca gttaagcgag attgcatagt agtaactaag
    21301 gaaattcctt ctttgtatgc aaattcaagt tgcttccaac gaaaaaccat gacaacgatc
    21361 tgagcaaaaa tccatataaa cacacctatc ggatttccat cagttgccat attcgccgcc
    21421 aaagccccaa aaatcgttgg aatcaaagca gcaaatacgg aatctccaat cgctgcaaag
    21481 gaacccatca atccagcttt cagccccgat acggtttcct ttgaagcaat tccctctttt
    21541 tcttccattg caagatcaat cccagtaatg attgtattaa aaaaattaga agtattaaaa
    21601 aattgcgtat gtgttttcat catctctttt aattctggtg tattgtctcc atatattttt
    21661 cttagttgtg gcaagatagt atataaatag ccagaaccct gcattctctc atagttccaa
    21721 cctaattgaa aagtgaacaa acttcttcga ttgatttgct taaaatcttc ttttgttaat
    21781 ttgtaattag atttcgtcat cttcgatttc cccctcaaca gaatgtgatg gctcactagc
    21841 taatggttga tggtctttgt tttgatcatt cttataactg atcattgcta gtgctagacc
    21901 aatcaatgct acagcaagca tcggcagcga attgaaagtt gccggaagat ctttaacaac
    21961 gcttgccaca cttgttccta ataattgaac attagagaat aacgttgcta ataatgccgt
    22021 gatcgtaaat cctaaaataa gataagatag atgttttttt actggtaaat agcgcagaag
    22081 aatggcaaac ccaacagctg gtaaaacagc tcctgctact gaaagtccat cgcctagcca
    22141 ttttagattc ccgtttaaaa cagaaacaac attttcaact aatccaccac caaatgctaa
    22201 agctaaaaat acaggaatca tacgagagag cgaccaaggt aaagctccca ttaagaaatt
    22261 gcgttcgatt gcttgataat tcatgtcttc tacgtgacgg tcaatacgat gggcaaagta
    22321 tgtgttagcg aatctagcta aaatatccaa ttggatcatt aagcttgcta caggcactgc
    22381 aatagcagca atcgcttgtt ctggattcat tccaagagaa atcgagaagg cagttgctaa
    22441 aatcgtccca gagttggcat ctatttttga tgcgcctcca aacgtcccca ctcctaaaac
    22501 tgtcaactgc atactgccac caataatcaa acctgcgttt aaatcaccca ttactaaacc
    22561 tgcatataac cctgcaaaca ctggcgcact cattgaagaa tagatctgta attcatctaa
    22621 aatttgataa cctgcatata atgttaataa taagatctgc caccattgaa tatccatgga
    22681 tcattcctcc tatttgatta aaagattcat aaagtcttct gctttatcgt ttggtaccat
    22741 ttgagcaatt aaatgtatac caagctgatc taatttttta aatacttcga tatctgcatc
    22801 taccacattg attgattttg taatggaccg tgtttcattc gtttgtgaca tattccccac
    22861 atttacttct ttgataggaa ctcctttttc tactaaagat aagattcgat ctggcttttt
    22921 tgctacgatc aatactcgtt gagagtcata tttgccagct aaaatatttg ttgctgcttt
    22981 ttcgattggc aaaatgctta attttacccc tgctggagtc gctaacttca atccactttt
    23041 ttcaattgtg tttgtggcta cctcatcatc cacgaccatg attcgactaa tattcagttt
    23101 cgttgtccaa agatttgcta cttatccatg tatcaaacgt ccatcgattc ttacaccaat
    23161 aattgccatc atgcattctc cttatctatt ttttctattt attgttcaat taaaactgtg
    23221 acatcatcaa atatcccttc tgtttcaaag ataacgtttt cattttttct ttcttatatt
    23281 tcatttttaa gctaagaaca aattccttcc caactaaaga aaacgctttc ttttatattt
    23341 aagttcattt gattttctta acttgaatgt gtttcatcaa catcgtaact ttcatagggt
    23401 cgatccagcc agttttctct tgttgcgcgt ttgccatacc acaagccata gaaaatgcga
    23461 gaatctcttc gattgattgc ctttttgata acgcatgggc cgatcctgca agggtcgcgt
    23521 cacctgaacc aacaggatta acaactgata ttttaggaat aatgacttcg taaaaacact
    23581 gagcaacttt tgccacagca cctctctctc ctaaagaaac aacaacccat ggcactcctt
    23641 caaatattgg atgagctaat aaggactgaa ttttttctaa ttgaatcgaa gaacagatac
    23701 gttggccaat cagttctcct aattcttcca gattgggctt gataagtagt ggcttctcct
    23761 taccttccaa tacttcaagc aaaaatttac cagaagtatc tagtaatatg tctttccctt
    23821 ttttatcagc taaagcaacc aattgcttat agtaatcaag agggcatcct tggggtaaac
    23881 tacctgagat cgtcataata tctgatattt cgaggagctg ctcgaaatga tcggtaaatg
    23941 tctgttgttc tttctttgtt atgaaggggc ctcgttccaa aagctctgtt tgtctcgaac
    24001 tatgcaggat agcaatagaa tcccttgtct ctcctttaat tggaacaaac tgatgcatga
    24061 tccctaactg atctagctgg ttctttagat attcaccgtg ctggccacct attaaaccag
    24121 tagctatcac ctcttctcct aataaattta gaaccctcgc cacattcact ccttttcctc
    24181 ccgctgtttt attgactgtt tcaactcgat tgactttgtc gaggtttaat tcttcaattg
    24241 tatacgcgat atcgatggaa ggattcaagg taactgtcgt aatcatttca tcttcacctt
    24301 tttgcttttt gttttattga accaaggggt agcggtttca tttattactt tatttaaagt
    24361 ctcgatgttt tctctcccat ttgttcttaa ccaatgaata gctccgtctt ttccattttc
    24421 gacgtatgct ttgaccccat ctttccatgt cgcacgtcca cacaatacac cattgaaggt
    24481 ggaacccgat tcatgagcaa aaatcagtgt ttgttgaaat aattctgttg aaacaccccc
    24541 actcaaaaaa atgaatggta atggagtcgc ttcactttgc tccttaaaga aggcagcagc
    24601 ctcttctttg ctgtgaataa attcacctgt ggaatagcca ttcacataat tcatattgac
    24661 tggtacttct actttcaaca catcaacttt atattttgga tcagaaaatt ctttcatcat
    24721 ctcaataact ttgtggggct tgactttcgc atactctgga gaagaactat cgcttatttg
    24781 tgcatcataa gaaactaact ccaaatagaa gggacgattt tctccttcgc actcactacc
    24841 taaacgttcg atgaacacat gcttcaaatg attgatttcg ggtgcttcat caacatcgta
    24901 gtaaagtaag aattttactg catccgcttg ctgttctttc aatctgagag aagaccaatc
    24961 cgctaataaa tcaggtagac gaccaggcgt agttgcgtca tagccggttt tctcataagc
    25021 taacaataac cccgtctctt ttgctctgac ttttgctgct ggcaatccat attccggatc
    25081 caataaaata gcagaagcat agggtgtcag ctcagctgat actagttctt tgaattcact
    25141 gatttggtct tcacttggct cttttcccaa cgatttgatc atttttttta gagcaccacg
    25201 ctgatcgatc gctaatgcac taataatccc atcttttgtt gatagtctct ccattgccct
    25261 atttttttct tcattcatct gtcatttctc cttcggtttc atacttatga ataatgacgc
    25321 ccttgactac tcgattgacc gtgcctgttg gagatggtgt atctggttta ttagctactt
    25381 tgattgaagt atagagtgca atgatttgtg cagtcataat catcgctaat gcaagatagg
    25441 catcgggcaa tttactcacc gagtcttcca atagcacagt catacctgag aaatcaatag
    25501 tacccggttg cgcaatcgca atgacattgg cagtgacccg atcttctttt atttcctcta
    25561 aaatatctaa gtcataattt cgcgtatatg gttgattgct cacaaaacct acaacgagtg
    25621 tctcgctatt taaaaaagac tttggtccat gacgaaaccc catcgaagaa tcaaaaattg
    25681 tagcaacttt acctgctgtt aattctaaaa tttttaattg tgcttctctt gtcgcacctg
    25741 ccaaacttcc tgaacccaga taagcgatcc gttcaaaaaa atcaggtaat aacttttgaa
    25801 tttcttgatc tcttgaaaga agatcttgac cgattttaac taattgttca agatatcttt
    25861 cttttttctc cattgctgtt tgatcaaaga tccataaagc actcaataac atacacgtaa
    25921 aacttcctgt catagcaaaa cctgcatcgt ttgctctctc tggcatcaat aacagataat
    25981 tctgtttatc atcctgtgcg ttttttgcca acatcccctc tttagcacaa gtaatcgtca
    26041 aatgaaacaa tgtttcaact tgttcatttg caatttgcac agccgctaca ctttctggac
    26101 tgttgccact gcgagcaaat gaaactaata aagtcggttc atttggaaac aaaaattctt
    26161 ttggtgaagc tacaatatct gtcgtgccaa aactgtggaa gataaaaaac tgattgtctc
    26221 catgttcatt tagatagggt aagagcgtat ccccaatata ttgagacgtt cctgcacccg
    26281 taaaaataac acgtattctt cttccgtttg cttctttttt tatttgatca aaaaatagag
    26341 ctaaattttc ttggctttgc atcaatctat cgattgtttc tttccagaga tctggttgtt
    26401 gttttatttc ttgtgtcgtg attgctgcac ctatttgttt taatttgttc ttcgatagct
    26461 caaacatgtt gttctccctc tcaatcattt tcggatatgc cgaattttat aatgaaactg
    26521 gtccgctctt gctacactat ctgtatattc gataatctca tttttcacgt tataggttat
    26581 cctcgtcaga tgtaagacgg gagcgccatc agcgattcgt agcacaacag catctttttt
    26641 ttgcacgata cttgcataaa actcttcatc agctaattga acttgttgtt cataatcctg
    26701 tgaaaacaca tcatacaacg gtttactttc aagtaacggc aaatcaagtc ccataaatag
    26761 tttcgccggt agataagaac gctcaaccat caatggctct tcatctgcca aacgcaatct
    26821 tttaagttta aatacagttt cacctaaatg gatgttcaaa ttcttagcaa ttctcttatt
    26881 agcttccatt tgtctaaatt ctaaaatgac cgtcttaggg acgcgcccta tttcgcgcat
    26941 ttcttctgta aagctatatg ctccagctaa gttcgatgac ttatcagaca aatcagagac
    27001 ataggtacct tttccatgtc ttcggtaaat ataaccttga ttttccaatt cctgtaaagc
    27061 cagacgtacg gtggtacgac tcaccccata gtgttgacaa agttctcttt cagataacag
    27121 cttatcatgc ggagtcatct gtgtttcgat acgatccttc aataaatcaa ctagttgata
    27181 atacaatgat tttcctttat ttgagctctc cataaaaatc acctcttctt tactggttat
    27241 aaccacaaca caagaatagt gaattattaa tcatttgtca aaggtttttt agaccaaaaa
    27301 agctattata acagctcttt aaagctttat atcttgaata gacaaagata cctcaaaaat
    27361 accagttacc aataaaagaa cagaaaaaaa gacgaaccac ataagcgatc cgtcaaaatg
    27421 gtctaactta tcgagcagcg tgatccacat taatccctat gatattccta caactatcgt
    27481 tttattactt ccaatatcat ttacaacaac taaaaatagc ttattaatga caaatgttat
    27541 tattagtttt agataacttt tgcagtttta tacttttttt ctataaaatt tgacaaacat
    27601 ttttttaaat aactaattat tttttgaatc tgtcactcag acaatccttt ctcttacttg
    27661 ttaacataaa tagaagttgc tgtttcttct aatagtgtac ttaattcttt aatattttgt
    27721 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggtgct
    27781 actcccttcg cccatgttgc tcgcccacat aagacaccat tgaatgtcga acctgattgt
    27841 tttgcaaaac gcaatgtatc ttgaaatagt gcagcgctga ctcccgcact taaaaaaata
    27901 aacggttgtt cagtcacttt cgattgttca tagaaatacg cggatgcctc tgcttgacta
    27961 taacaagtat cttgcccata tccttccaca aatctcatat tcactggtac ttctactttc
    28021 aatacatcca cttgataacg ttcttttgaa aattctcgca tggcttcaat cactttatgc
    28081 ggttttactt ttgcatagtc aagagaagac ggatcgccaa tagttgcatc gtaagaaagg
    28141 agctctaaaa agaaaggtat gccttctgct tcgcattctg aaccaatccg ttctataact
    28201 atttgttttt gcacattaat ctctactggt tcatcaacgt catagtacaa taaaaattta
    28261 caagcatccg cacccacttc ttttaacctt ttcactgacc aactattcag taaatctggt
    28321 agtcggcctg gcattttcgc atcatagccg gttttttcat aagctagcaa caaaccacat
    28381 tctttctctc gaagcgctgc tgcaggcaga ccatattctg gatcgagtaa aatagcggaa
    28441 gcatacggag tcaggtactg agaaaccatc tgtttaaatt ggatgatatc ttctgatgta
    28501 gcttctttat attggttgat cattttcttc aaagcaccac gttggtcgat agctagcgcg
    28561 ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt cctgtttttc tatactcatt
    28621 ttcttcatat atcttcacct atttctttag ttaatattgt agctatttct atttttgatt
    28681 ttgcagctct caactgttct gtaacctctt ttcgcatcaa tgatcgtgca acgagagaca
    28741 ataatttcaa atgagtcgtt cctgcttctg aatctgggat aaataacgca aaaataaaat
    28801 caactgactg tctatctaaa ctgttccatt caattccttt tttcagtgtg acgataatca
    28861 aactaggttc attgattgtt tgagacttcg catgagggat agcaaaacct gacattattc
    28921 ctgtagtacc ttctttttct ctttccagaa gtttttgata tacttctttt tcgtcatgag
    28981 cgattcctaa acagaatgtt tgctttgcta aaaacttaaa tgcttcttct tgattatcta
    29041 atggctgttg taaaaaaatg ttctcttcat gaatgattgt catgataaac ctcctgctct
    29101 tccttcttcg ataactgtac gatttgttgc tttattatcc ctcttcttgt ctttatcagt
    29161 tactcttttc ttttggatca aacctagtat cactccacca acgatcgaac caataagaat
    29221 cgctgtaacc cattggattt ttcccgtcac tacaggtaac actaaaaaac cgccatgtgg
    29281 ggctggtact tggacaccaa acagataggt caagacagca gcaattgatg agcctaacat
    29341 catcgttggt aatactcgga tcgggtcttt cgcagcaaac ggaatagctc cttccgtaat
    29401 atgagttgat cctaaaataa aattaaccaa acctgcatta cgatcattag tatcaaaata
    29461 tttaccaaac aataaagtag caaaaccagt aatcaacggt ggtgcaatgc aagctgctga
    29521 cgcaccagcc ataaaagtcg tgtttccttg tgtcaaaaga gctgttcctg ttacatatgc
    29581 cgccttatta accggtcctc ccatatcaaa cgcacacata cagccaacaa tcacgccaag
    29641 aacaagtgga ttcgaatatt caaatccagc taaaaagctc atcattcctt catttatagc
    29701 cttcattgga cctgccagca aagacatcac gatgccaata aatgctacac caataactgg
    29761 atataagacg attgccttca agccatctag ttgtttaggt aaaattatca gtagcttttg
    29821 caaaaataag ataaaataac ctgctaagaa cccagctaaa atacctccta aaaatccagt
    29881 accgccattc atggctacga ttccaccaac aaatccaacg actaaccctg gacgtttcgc
    29941 gatcccttct gcaatataag ctgctaaaac tggaaccatc attcccattg ctgcatcgtc
    30001 aactgctttt accattgccg ctgtagcatt gtattgtgca tgatctggat cagaagaata
    30061 gattccccaa acggcaaatg aaatagcaat caatactccg ccagctacca cgaaaggcaa
    30121 catatgagag acaccgttca tcaaattttt atagatgcta cgaccaaaat tagcaccact
    30181 tatcgcttct ggttctaaag tatcagcagc ttcattatct gctaagacag taccttttcc
    30241 tgccaatgct tcgtcaatca gccgttgtgc atccttgatt cctgtactga cagaaacatc
    30301 aatgactcgt tttcccacaa atctttctga atggacatct ttgtctgcgg caataatgac
    30361 agcatctgcc tgtttgattt cttcagccgt taattcattc tcgatcccaa tttgtccatg
    30421 ggtctccact ttgatcgtga tgcctttttt cctagctgct tgctctaatg cctcttgcgc
    30481 catgtaggtg tgcgcaattc ctgtcggaca accggttgct gcaattagtt gatattttcc
    30541 catctttcga acctctcttc cttgattttg atttgtttac ttaattcctc cacatccaaa
    30601 aaatcagtca gtcctttacg aaaagcagta gagctgcctg ctgctatgct tttacgtaac
    30661 gtttcatcaa gcgatcgtcg attcatgtat cctgctaaaa aagtgcctaa catggcatca
    30721 cctgcacatg ctgtattgac aacttttcct tttggcgagt ttcctcgaat caccctgtct
    30781 ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca aaacattttc tgcgcctgac
    30841 tctactagtc gttgtccata aacaaagtaa tcttcatctg taaccatttt tcgtccgaac
    30901 caagaagaaa gttcttcctc atttggtttc agcaaaaacg gtcgataggg taaacaatcc
    30961 atgacttctt gatcactact atcaataatc aaacaaatcc gactgatttc tattaaaacc
    31021 tttggagaca gccctcttgg taaactccca gaaacacaaa gataatcccc tgcttgtaat
    31081 gctcttattt gatttaaaaa attatgaaca gctgtctgcg gtatttcagg tccttgattc
    31141 actaatttgt attcttctcc cgtttgattg acttgtgtaa atacattgat acgcgtcata
    31201 ccaggcactt caataaactc tgtttggatt tgtttctgct gtaaatagtc ttctatatac
    31261 tttcctgtga acccagcaga aaaacctaat gccgtacttg ggaaatctag catctttaat
    31321 actagtgaca cattcacccc ttttccgttt gcctgaatgt catcctcttt tgtgcggttt
    31381 accataaaag gatgcatctc atctgtttca ataaatagat caattgccaa attcatcgtg
    31441 catgtgtaga tagacataac caacgacctc cttgattcta ttatgaaata cgcgaaatag
    31501 aaaatgtttt cttaatcgtc ccaacacaaa gaaaaccttt acatttataa actcgtcgtg
    31561 ctttttttaa ctaacttttg gtgaaagaag gaaagaatat gaatcacaaa ttaagtgaaa
    31621 cagagcagta cctctggaac tttatcgaac acctcatcct agagatccct aactattcca
    31681 tcgtgaaact aagtgaacaa gcaaatgtct caacagccac cattgtgcgg acaatgaaaa
    31741 agaaaggcta tgaagggttt acttcgttta agcatcatct aaaagaagga gaaaacaaga
    31801 acatcaattt ttcattttta gataaagtag acaaaggaat ccgccgagcc attctaaaaa
    31861 atgaacaaga agtggtacgt acaatcaata tgttggaaat cgggaacatt gaagatgcaa
    31921 tacaaagaat caaatttgct gaccgggttt ttgtctttgc ccgaggattt tcagaaatga
    31981 ttggccaaga aatgctggta aaacttcaat taacgggaaa aaattgtcag atgcatactg
    32041 atccagaaat tatcaaaagc atcagtcgga aactaactaa gaaagacagc gttatttttg
    32101 tttctttgaa cggtgagaaa acaaataaaa acatcaaaaa taagatttat gtctttattt
    32161 ttcaa
//
LOCUS       DAZAOG010000014        31645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000014 DAZAOG010000000
VERSION     DAZAOG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.014"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(231..2123)
                     /locus_tag="ACHDLD_000619"
     CDS             complement(231..2123)
                     /locus_tag="ACHDLD_000619"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362375.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2120..3847)
                     /locus_tag="ACHDLD_000620"
     CDS             complement(2120..3847)
                     /locus_tag="ACHDLD_000620"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362376.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3863..4513)
                     /locus_tag="ACHDLD_000621"
     CDS             complement(3863..4513)
                     /locus_tag="ACHDLD_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN3362377.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4717..5013
                     /locus_tag="ACHDLD_000622"
     CDS             4717..5013
                     /locus_tag="ACHDLD_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3362378.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5185..5805
                     /locus_tag="ACHDLD_000623"
     CDS             5185..5805
                     /locus_tag="ACHDLD_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3362379.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5897..7063)
                     /locus_tag="ACHDLD_000624"
     CDS             complement(5897..7063)
                     /locus_tag="ACHDLD_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3362380.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7075..7428)
                     /locus_tag="ACHDLD_000625"
     CDS             complement(7075..7428)
                     /locus_tag="ACHDLD_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN3362381.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7556..8578
                     /locus_tag="ACHDLD_000626"
     CDS             7556..8578
                     /locus_tag="ACHDLD_000626"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN3362382.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8597..8758
                     /locus_tag="ACHDLD_000627"
     CDS             8597..8758
                     /locus_tag="ACHDLD_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362383.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9017..9451)
                     /locus_tag="ACHDLD_000628"
     CDS             complement(9017..9451)
                     /locus_tag="ACHDLD_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362384.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9561..11420)
                     /locus_tag="ACHDLD_000629"
     CDS             complement(9561..11420)
                     /locus_tag="ACHDLD_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3362385.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11421..11561
                     /locus_tag="ACHDLD_000630"
     CDS             11421..11561
                     /locus_tag="ACHDLD_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN3362386.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11538..12698)
                     /locus_tag="ACHDLD_000631"
     CDS             complement(11538..12698)
                     /locus_tag="ACHDLD_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3362387.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13005..14429)
                     /gene="araA"
                     /locus_tag="ACHDLD_000632"
     CDS             complement(13005..14429)
                     /gene="araA"
                     /locus_tag="ACHDLD_000632"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN3362388.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14445..15143)
                     /locus_tag="ACHDLD_000633"
     CDS             complement(14445..15143)
                     /locus_tag="ACHDLD_000633"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN3362389.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15100..16761)
                     /locus_tag="ACHDLD_000634"
     CDS             complement(15100..16761)
                     /locus_tag="ACHDLD_000634"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN3362390.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16979..18379
                     /locus_tag="ACHDLD_000635"
     CDS             16979..18379
                     /locus_tag="ACHDLD_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN3362391.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18631..19710)
                     /locus_tag="ACHDLD_000636"
     CDS             complement(18631..19710)
                     /locus_tag="ACHDLD_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3362392.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19796..20755)
                     /locus_tag="ACHDLD_000637"
     CDS             complement(19796..20755)
                     /locus_tag="ACHDLD_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN3362393.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20767..22278)
                     /locus_tag="ACHDLD_000638"
     CDS             complement(20767..22278)
                     /locus_tag="ACHDLD_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN3362394.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22300..22863)
                     /locus_tag="ACHDLD_000639"
     CDS             complement(22300..22863)
                     /locus_tag="ACHDLD_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3362395.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22869..23732)
                     /locus_tag="ACHDLD_000640"
     CDS             complement(22869..23732)
                     /locus_tag="ACHDLD_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362396.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23746..24642)
                     /locus_tag="ACHDLD_000641"
     CDS             complement(23746..24642)
                     /locus_tag="ACHDLD_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362397.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24817..26106)
                     /locus_tag="ACHDLD_000642"
     CDS             complement(24817..26106)
                     /locus_tag="ACHDLD_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3362398.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26078..27595)
                     /locus_tag="ACHDLD_000643"
     CDS             complement(26078..27595)
                     /locus_tag="ACHDLD_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN3362399.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27793..28683
                     /locus_tag="ACHDLD_000644"
     CDS             27793..28683
                     /locus_tag="ACHDLD_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN3362400.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28756..29580)
                     /gene="nadE"
                     /locus_tag="ACHDLD_000645"
     CDS             complement(28756..29580)
                     /gene="nadE"
                     /locus_tag="ACHDLD_000645"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN3362401.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29592..31064)
                     /locus_tag="ACHDLD_000646"
     CDS             complement(29592..31064)
                     /locus_tag="ACHDLD_000646"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN3362402.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9261 a         6642 c         5474 g        10268 t
ORIGIN      
        1 ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtaaaatcag
       61 cggtttgacc tgagtattag agatacaaaa cggaaaaata gcttctcata ttttgccgtt
      121 ttttgagcta ataccgaagg acagctgatt gaacaccgtt tattcggaaa aagggactgt
      181 gacacgactt ttgtcacagc ccctaaaaaa aataataaat ttgtactatt ctatgaagct
      241 tcttcttcag aaaactgact gttataaaga tctgcataga atccgtcttt ttccatcaat
      301 gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca tcacaataat gttgtctgca
      361 tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg tccggttttc caataggcgg
      421 ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta cactagatgt tgcttcatca
      481 aggatcagga catctggatt tgctaagaat gcccgcgcaa ttgtgattag ctgacgctgc
      541 ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt tatatccttc tggcaattta
      601 cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa tgatttgttc gtctgttgca
      661 tcgtcgttcc catatttgat attgtcatag atacttcctg taaacagcca agtatcttgc
      721 agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa gatcacgggt atctacacca
      781 tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt caagaaggtt gatcagtgtc
      841 gtcttccctg caccagtcgg acctacaata gcgaccatct caccaggttt gacatccaag
      901 ttaaaatcag tcattagcaa gttatctggt gtgtagccga attgaacatt ttcaaaacga
      961 actttgtatg gtgtatcttt ttcttctgga aggccagatt tttcattgga catctcttct
     1021 tcatccaaca cttcaaatac ccgttcggct gaagcaactg ttgattggat agtgttcatc
     1081 aaacttgcaa gttgagtgat tggttgagag aactgtgttg tatattgcaa gaacgcttga
     1141 acatccccta atgtcatgtt cccatttgca acttttactc cgcctagtac agcgacgaac
     1201 acatagccta gatttttaac gaaattcatc aacggcatga tgattgccga aataaattga
     1261 gctttccaac cagcggcata cagattctga ttttcttttt caaatacttc ctggtctttt
     1321 tcagtatgat tgaatgtttt aacaatggta tgaccaccat atgtttcttc tacttggtta
     1381 ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca cttgtgattt cggcgctacg
     1441 atcatcacga tgatcaagct taaagggatc atcaataacg cgactaacgt caattgccag
     1501 ctgattgaaa gcatcatcac gatgacccca atcaatgtga ctgcactagt tacaaattga
     1561 gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat cattgattgc tcgagacatg
     1621 atatccccat ttgtatgtgt atcataatag gaaatcggta cacgattcat tttggcttcc
     1681 aaatcatgac gtaattcata aactgttcgt tgggaaacac gtgtcataat aaattgctgt
     1741 aaaaagttga atacagcaga aatcaaatac atcacgatta ctgtcgcaat gatctgcgcg
     1801 attttatcaa agtctatcgg aaattttgtt acttgttggc cttgttgcat ctgttgagcg
     1861 ccagccatta cacctttgaa tatttctgtg gttgctttcc ccaagacctt tggtgtttgg
     1921 atttggaaga tcacggctgc tattgctagt acaaatacag cgatgatcgc aaccatacgt
     1981 ttagacatat agccaaacaa tcggcgtacc gttccccaga aatttttagg cttttcacct
     2041 tttacaccga gatttctgcc aggtccacca tgtcctggac cgcctgctgg tccatgagag
     2101 ggacgtgtgt ttgttttact catgctaagt cctcctctct tagttgtgaa tgaacgattt
     2161 cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt tcctttgcca accagttttc
     2221 cttcatctag cacaaggatc atatctgcat ccattactgt acttactcgt tgagcaacta
     2281 aaacggtgat actttctttc atattcgttt tcaatgcttg gcgaagattg gcatccgttt
     2341 tgaaatccaa cgctgagaag gaatcatcaa acacatagat atctgctgtt ttgactaatg
     2401 cccgtgcaat tgccagacgc tgtctttgtc caccagagaa gtttcctcct ccttgttcca
     2461 catggctatc taagccatct tccatctctg aaacaaaatc tttagcttgt gcgatttcta
     2521 atgctttcca aatcatttca tctgttgcat ccggcttacc atactgcata ttgtctcgga
     2581 tcgttccaga gaacagaact gctttttgag gaacaaagcc cattaattct cgcaaattat
     2641 attgagtcat gtcactaatg tccgttccat ttatttggat cgaaccagat tcgatgtcat
     2701 ataatcgagg gatcaaattg actaaggtcg ttttacctga accagtcccc cctatgattg
     2761 cgacgatctc gcccgacttg gcttggaaat caatatcttc caatgcaagt ttttctgctc
     2821 catgatatcg ataattgaca tgattgaagg ccaatgttgc gttttttcca gcaaaagaaa
     2881 ctgttttagg attttcaggg tcatggatac cgattttttc atccaacact tcattaatac
     2941 ggtcagctga agcttgtgca cgaggcacca tgataaaaat agcggaaagc atcataaagc
     3001 tgatcaagat ctgcatcgca taagtcataa atgcaatcag attaccgact tccaaagtca
     3061 tatctgctat gtagtgtcca ccaaaccaag tgattgctac gtttgtaccg ctcatgatca
     3121 atgtcatcat tggcatcatc aatgcgacga tcgtattaac tttgattgcc gtatttgtat
     3181 aatctttatt cgctaaatca aaacgatttt cttcaaagcg tgtcttatcg aatgctcgaa
     3241 tcactctgac ccctgtcaat ccttctcgaa ataccaggtt taaacggtca gttttctttt
     3301 gcatactttt gaataatgga acagcaaagt acatgattcc gccgatcaaa ataatcatta
     3361 ctgggatgac aactaagaag atttttgtca actgatgatc tttttgataa gcaagtatgc
     3421 tcgcgccaat caacgtgatt ggtgcattga tcatcatacg taaaaacatt tgtgtaacca
     3481 tttggatttg agtcacatca ttggtcgttc ttgtgatcaa agaggccgtt ccatatttat
     3541 caaacgcatc ttttgtcaga ctctctgatt ttttatagac atccgtacgc aattgcttac
     3601 ccagtttctg tgattcacga gtagcaaaaa atgtatttgc gatagcagct aagatactga
     3661 tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat ataatcgaca tctccttgcg
     3721 ctaccccttt atcaataata ttggaggtaa gtgtcggcaa atataaatct gccagtactt
     3781 ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa ggagatccgt tttacgattt
     3841 ttagcatcta gttcccttct ttctatttat atactccgtt tttcagccaa gaaagtttca
     3901 aattgaattc actgattgct tgctctactg agtaatcttc agatgttgaa aataagcgat
     3961 acgaatgcgc aattgttgta aatacagtat tcatcactaa ttggatcagt aattccaatt
     4021 catgatcatt ttttactttc agcttcgtcc gatcgatcaa ttcaagcaac tcttttgcat
     4081 cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg gcgactcgta tgaggagcga
     4141 cttttcttga ggcacggtaa tccatgttca taaacaagtt acgataaaaa gaagcattct
     4201 cgccttccaa aatctcataa atcattttag aaaaatagga ttcgaatcct ttgaatagat
     4261 caccatcgga ttctttcatt agttgtaaca tatctttaga gctgtcacgt ctcattgtct
     4321 caaagtaata aaaataaaga tcctctttat cttcaaaata ctgatagaaa ctgcctctag
     4381 ggatatccgc taatttgacg atattagcaa tcgaagcttc ttttaagggt actctagaaa
     4441 actcgattct cgcagcctcg attagtcttt tttgtttttc tttaggcaaa tggaaaaatg
     4501 tctgtgtcgg catatggatc ctcctttctt tcaagatgac acattgtcat atgacgctgt
     4561 gtcattataa cacctcctac ctgttttgca agacatttgc atagtttttt tcattatcat
     4621 ccgatacatt aaaaaagtga taagaggaat ccttccattt gaattaatga gagttgccca
     4681 ttaaaatagc cacatactaa acaaaggatg agataaatgg cccaatcaat tagtgtccaa
     4741 gacttcaata aattgcctct cgatgctgac agcatcgttt tagatacaag aaatacctta
     4801 gattacacaa aaaaccactt aagccactcg ctttctatcc ccgctgctat cttgccaaga
     4861 caattaaaaa atctagatcc aaagaaactt tattatattc taagctatac aggcaggcga
     4921 tcagaagcat tagccgatca acttactgct aatggttttc gtgctgttta tatcattggt
     4981 gggatggaag ctcttcagtc gagtgttgct tgaatattgc ttgttagatt caaaagaggc
     5041 tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt ctttttttag ttatttattc
     5101 ttcaacctta tcaatatgac ttgatagcct tttctttttc tcatagttat aacaatcata
     5161 gcaataaaag gagaaggtaa gcatatggca tctgttttag tagtcaaagg tcatccccta
     5221 actgcagaag aatcgcgcac agttaaggca ttgacgtctt ttttaacaag ttataaagaa
     5281 aatcatccag atgatgaagt gactgttttg gaactttatc gcgatgatat tccggaaatc
     5341 gatgaagaat tattatcagg ttgggaagca ttacgtgctg gtgcagaatt cacttcctta
     5401 tcagaaagtc aacaacaaaa aattgctcgt ttcaatgaat tgactgatca gttccttgct
     5461 gctgataaaa tcgttatcgc aaacgcctta tggaatctaa atatcccaac aaaattaaaa
     5521 gcttggttcg ataccgtcaa tgtagctgga aaaacgttcc gctatacaga aaacgggcca
     5581 gaaggattag tgacaggtaa aaaagcactg cacatccaat ctaatggcgg tgtatacaac
     5641 ggacaagact ttgcctctca atatgtaaaa ggtattttaa actttgtagg catcgatcaa
     5701 gtagatcagc tattcatcga aggaatcgat tatgaccctg accgtgcaga tgagttgatg
     5761 caaaatgcat tagataaagc tgctgcttta ggtaaatcat tttaaaaatt gagataaatg
     5821 catacaaaag agacagagga aaaaatcttc aaagcagcat ttgtaccttg gatattttcc
     5881 catctgtctc tttttgttat tctttctgct ttttcaacgg attttcttca taaagagaag
     5941 agattgttgt gtaccattca aaaacatgag taatcactac tttagctgct gcataaacag
     6001 gaatccccaa gatcacaccg atgattccga acaatttccc agaagtcagc agaactaata
     6061 aaatcgttac tggatggatt gctaactgat tccctagtac taatggagag atcaagcgtc
     6121 cttctattgt ctgctctact gcaaaaacga tcagtacttt gatgatcatg aatggacctg
     6181 ctacaatggc taaaaagata gccggaatca tcgctaaaaa tgatcctaag taagggatca
     6241 ggttcaaaaa acccgcaata atccccaacg tgacagcata ctctaaacca atgaccgcaa
     6301 aaccaatcat aaacatgatt gccacagcaa aagccacagt tagttgtcct cttatataag
     6361 aagaaacttg actattcatt tcactcagca cattcaacgt cggtttttgc atgcgtgtcg
     6421 gtaaaaattt gacaaaataa ggtgctaatt gtttaccgtc tttcaataga taaaataaga
     6481 taaacggcat cgtcagcaga gccactaaaa tagtcgctac cgcgccgaca aaacttccaa
     6541 gactctgaac tgtactctta gatatatctt ggatcatatc gcctgccgaa ttcatgaact
     6601 tatcacctgc tgtttccaac tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct
     6661 cggttaattt attatcaatc gtatctacat attgagggaa gttactgatg aagctcactg
     6721 tttgttcctg aatcttaggg atgatcacta cgctccccca tgcgattagt gcaacaacca
     6781 aaacaaacag cccaatgata ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa
     6841 ctaccggatt catcaagtaa tacaagattc ctgctaaaat gataggcaga cctacgattg
     6901 ctaaaaattg ccagattggt gcaaacaaat atgaaacttt tgtaaataaa aagacgatca
     6961 gcaatattaa caatacaaca agtaaggctg tgactacttg attatttaaa aaccagcgcc
     7021 aaaaccacga taccggtctg ttcttcttat tttttccttc cataattcct cctgttagtt
     7081 ataaaaaatt tggcttccca aaccaatagc aggcagtcta tgcggatgaa gatagattcc
     7141 atttgcaatt tgtccttctt ctggacaagc atcaaaaatc aatgatacgt gagctactgt
     7201 ctctaaattc ccattggcca tcggaccgac ataatcgatc gtatatacct gatcatcaaa
     7261 agcaattgtt ccgcctgctt tcaaagaaaa aggttcgtct tttgatactt cttgaatcac
     7321 cgaatatttt cgtaaggcag ctgttgctgt tttatcgaac aaaatcagca tgggttcttc
     7381 cttgtttatt gcttctgtcc caatttctgt aatctttgcc tgaatcattg cactctctcc
     7441 tttaattgct ttcaaaaaca gtataacaca ttctcattct ttagccgtgt ggaataatta
     7501 tgtgatagaa taaaaacgaa aactatttca ttacaattag gaggaatcag ctcatatgat
     7561 cgaaaagata attttaggaa cctatacgcg tcgcgaaagt aaaggaatct atacgatcga
     7621 ccttgataca gaaaaagaag aattagccaa cctttcatta gctgttgaag aaaatagccc
     7681 tacctatgta gcaaaaagta aagctggcaa tctttacaca gttactagtg tggatggatt
     7741 aggtggtgcc ggtgcgtacg ataaggattt tcattttttg aatgcggtaa cagagtctgg
     7801 cgctcctttg tgttatgtag cggttgatga agcacgtcaa ttactatacg gggcgaacta
     7861 tcataaaggt gaagtaaatg tctaccatat attaggagat ggcgaactga aagcagcaga
     7921 ttcaatattc catcaagaag cgacaggtcc tcataaaaac caagatcatg cgcatgtcca
     7981 ctatacggat ctgactcctg accaacgatt agtcgtatgt gatcttggta cagatcgtgt
     8041 gtatacttat gatgtgtccg aaaacggcaa attgaatctt gtaactacct tcactgcaga
     8101 gccaggaaca ggccctcgcc atcttgtctt tcatccaaat ggacaattcg cttatttatt
     8161 tggtgaatta gacagcacag tccgcgtgct ttcttataat aaagaaacag gtgcttttga
     8221 agaattacag aaaatatcga ccttgccaga agaattcgac ggtgaaaatg gaggggcagc
     8281 tatccgcatt tcaaatgatg gcaagttcct ttatgcttct aaccgaggac ataactcgat
     8341 tgctgtattc gcagtttttg aagatggttc ccacattcaa aacaagcaaa tcatctcaac
     8401 agaaggggat tttccccgcg atttcgcact agatccaagc aataattttg tcgtgtgtgc
     8461 caatcagaac accgataatc tgacattata tcgccgtcat cctgaaacag gattactgga
     8521 attactgaaa aaagatgtct atgtaccaga atgtgtctgt gtttattttg atcgttagta
     8581 agaaaaggag aatgaaatga atcagcaaga cgtattagac agactacggg aagaactaaa
     8641 tattcctttc tttgatggga aaatcgaaga taaagagtat agcgaagagg aatatcagaa
     8701 actcaaagcc gatttacaga actactttga acaatatgta cagaatgtag aaaactaatc
     8761 agctttccat actatctgaa aagaacatca ggacaagact tttccgtcta tcctgatgtt
     8821 cttttctaca aataaaaaac cgccgagatg gttagactcg acggaaaagg agtatttact
     8881 cgattttgag taagtacaag gttgttgttg gttataggta tgtcattatc ataaaggatt
     8941 ttgatcactc tatcataaag gtaatattac atttatatta gataactcta gtttttattg
     9001 ttatcaaaga tttcttttat tttttcaaca acaaattagt cattttttgg atggcagctg
     9061 tcaattggtc tgtcgcttgg tcttgttttg cttcagatgt attgaataga tcttcatcta
     9121 cccctgattc aagatcgata ctttggatca tttcttcaca gcctgctaca tatgaaagat
     9181 aagctttttc aaaattttta tgaatgccca ttgcttttgc tggtggacgc aaagtactga
     9241 ctttcttaag cattgcccga tatttttttg ttccttcttc aaataacagg atgatctctt
     9301 caagagtcga tttgtccagt tcacttgttt ttccttcatc aattgcgttt ctgatcactt
     9361 catatttcgg atgcattcct tctccgattg cttctgtttc cttgacgatt tgatcgatgg
     9421 actgcacata atagccaata ttcggtaaca tgttcgtttt cctactttct ccattgttgt
     9481 tattatttat tctacttgtt tttatcctca aatgacaagt acttgaaaaa atttaatggt
     9541 ttctatacag ctcgccattc ttattcgtgc tcaaagaaac catctgctaa actatattgt
     9601 ttccctgcga caatgtcata ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag
     9661 cgattgaatt gcgcttcttt ataccactgt ccgttttgat agtacagttc tcgttcaaac
     9721 gccggaagtt cctgttctaa tgataaaagc agctgataaa agccatttgt tgtctgattc
     9781 gtctgttcca ttagattcgc tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt
     9841 tgcttttcta attctccttt actgtcgaac ataagaaatt gtgtttcatg caatgtttgt
     9901 ttgtcatttt ttgcttggat cgtatctgaa tagatccctg gaagatgatc cccccaaaaa
     9961 acaactaaag tccttctaga aagattcttc aattcttccg tgaactgctt cagtgccgtg
    10021 ctgctatagg aaatatcttg cagataattc tctaagtcaa gtgtcccact tccatctgaa
    10081 gtttttgcag gatagtctag attatcatac tttccatcgt agggcatatg tgtctgcatc
    10141 gtgactaaat gaatgaactg aggtgttttt tcttccttga gaagtgtcat tacttctttg
    10201 taagctgact catcagaaat atatggatta ttctctatcg tatccgtata ggtcatcgta
    10261 cgctcgctga tgaactggtc aaatcctaat gtctgataaa catcctctct tttatacatc
    10321 gaagtattgt atggatggat cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg
    10381 atagaaggaa gctgatccat ttttggtaca agcatcgtgt aaggggtggt gagttgcgca
    10441 ttaaataacg ccatagaaaa accagtcaat gcttcaaatt cgatatttgc tgttccaccg
    10501 ccatagtttt gagaaagcat attgccacta tatgtttgat cagctacttc ataataatct
    10561 gccaatggct caccagatac ttctacgccg tttaatcgac taggatctga gaaactttca
    10621 ctcatcacga acacgatgtt cggcgactct tcctctacag ttttattttt ttcatcggct
    10681 agcttctggt atttttcagt gatttctttg atcttttctt tcgaatatcc ttcaggttca
    10741 tccattggtt ctaccttcag attgtacaaa aacccaccga taaaaccagt gttatagtag
    10801 ttcattttct ggctgtaagg tatccatagt gccgttttat tatatgcttt tcgcaacaaa
    10861 ttatcgggat tgttgaaatt actgatatac cctagcagtc caattgccac aagcagtgtc
    10921 gttaatcgga tcaattggaa tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa
    10981 aacaatccta tcacggttcc tgcggcagct aagatcactg taaacagcat agtgccggtc
    11041 atatctttca gcaatccgat ttcagtgatc atcttcaaat cgtctggata aatcggttct
    11101 tgccgataac tcattttcat ataatttgca aaacctaata caccgatggc tacgacatac
    11161 gtaagggaac ctaaagggac agatcctgct agtgcaatca gaaaaataag aataatcagc
    11221 aagacgagac aggctaagaa gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca
    11281 aggtctacgg atagatcatt ttgacaccac tgcaaaaata aattactaaa aacaacaaga
    11341 aaaaagatac tgatgattgc tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt
    11401 ctgcttatat atcttttcaa atgcttgctc cttcatttct atcaataaaa gaatgttgct
    11461 tctcttattt tagttgtacg aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat
    11521 ccaaatattc tctttcttta tggcctttct ggtttcttta ataagcggac ctttcgtact
    11581 ccagaaattt tttctagctg ttgtttcact tgattgagct gttcaaaatc agtactgtct
    11641 atgtcaatca atatgtagac atgctcattc ttatggttac gcgatatatc tgcgatcgct
    11701 agctcgttct cattcagcag agaaaatatc tctgctaata tattggtttt cttttgataa
    11761 aatatagtga atcgaaacgg cgcttggaaa aacattcgtg cggatggata attgaccgct
    11821 tctctaaccg taccgaacaa aagataatct tttaaggctt caactgccaa acgtccgctg
    11881 tctgccaaag cttcctgagt cgatccccct agatgaggca acataagtat acggtcattt
    11941 tctaagaact cttcttctgg aaaatcagtc aagtatttag ccagatgttg tttttccaat
    12001 gcttgcataa cagcatattt atcaacaatt tcacttcttc ctacattgat aagtacagca
    12061 tcttttttca ttttttctat attcgcctga tcgatcaatc cttttgtatc ttctgtcaac
    12121 ggcagcatga ctacgatgta atccgaggta gaaagaactg tttctaaatc tgcttgccga
    12181 acataatcca actgggcatc gcggatagaa taacccaaga catccatccc taagctatag
    12241 caacttagcg ctacttttgt gcctatcgct ccaagaccaa gaagaccgat ggttttgcct
    12301 tgcaattctc gtcccacata agcacttctt ttgttttctg cctgttctaa aatattagga
    12361 cctgttagtg tttgtaccat acgacttgct tcgattatcg gacgggaaga taagagcaaa
    12421 cagcacaaaa caagctcttt tactgcatta gcatttacac ctggtgtatt catgacgatc
    12481 gtcccgtttt cagaagcttt ttcaacgttg atcgtgttca ccccaacacc agctcttgaa
    12541 attgctagca aatctggtgt aagccattca tctttcactt tcgtactacg aataactatt
    12601 ccttcggctg gcaaatcatc tattattttg aaatattccc cttgtaactt ttctatctct
    12661 tgttctggaa tagcatcaat caaatgaacg tgatacataa aaccccctcc ttactaacaa
    12721 gtatctcgaa atttctgcaa ttagacaact gtttaccatt tgacttccta gaagcaaaaa
    12781 gagcagttag agcagagtag gctgagtgat aaaaataagg ctatgacaag ttactgccat
    12841 aagccttatt ttcgagagca aaatattgag ggtgagatat cttgataaat ggtgttccgt
    12901 tagctgacat tgataataag actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc
    12961 tcttcttatt tcttaaaact aagtggcttt ttcacaacct ctttttatct aataaccact
    13021 gtttccaact caactaattt tgcccacgtt tcaatttgat ctgttgttag atttagcgag
    13081 acaacggtat gatgaccgcc acctgcctga atccatgaat ggacaccttc atggaaattc
    13141 ggtttagttt tccataacac acgtgcaaca ggtaaattag gtgcttcagt cgttggttca
    13201 aaggcttcta cttcattgat cagcagttta taatgcgtac cgaaatctgc catcgatacg
    13261 actacccctt ctcctgcttt cccatcaaat acaagacgcg ccggatcttc tctatctccc
    13321 attgataaag gagaaacaac gattcttggt ttcgtcgcag caagcgtcgg atctacttcc
    13381 atcatatggg attccaagat tgcttcttgg ccactagcta gctcgtaagt ataatcttcc
    13441 atgaatcctg tatctttccc tcgagccatg attttcagca accgatcaat cgctgcagtt
    13501 ttccagtcgc cttccccagc aaaaccgtat ccttctgcca tcaaacgttg tacagctagt
    13561 ccaggtagtt gtttcattcc atacaggtct tcaaaattag tcgtaaaagc gttatagcct
    13621 ccttcttcta agaaacggcg aataccgatt tcctgctgag cttgtacttt tacatgttcc
    13681 tcccatgtct tttcttcata atcaccatag tcgaaggtat acaattcctt gtactcttca
    13741 aataattgat cgacttcttc gtctgatacg cgatcgatta cttgtacaag atctccgata
    13801 ccaaaataat caacagtcca accaaattga atctgtgctt ccactttgtc tccttctgtg
    13861 acacccacgt ttctcatgtt gtcaccaaaa cgagccactt ttattccaaa actttcatta
    13921 taggctactg caacatccat ccaatcagca atctcttttt ggatctcagg tcgtttccag
    13981 tagccaacga cgattttatt ttgtttattt aatcgagcat tgatgaaccc atattcgcgg
    14041 tcaccatgag cggattgatt taaattcatg aaatccatat cgatcgtttt ccaaggaatg
    14101 ctttcgttat attgcgtagc taaatgaagc aatggttttt gtagaagctt tgtcccgcgg
    14161 atccacattt ttgctggtga gaaagtatgc atccaagtga tcacacctgc tacttcttct
    14221 cgataattga cctctttcat gatttttgtg atcgtatcag cagtaactgc taattcttga
    14281 aggcggattg gataaggcaa ttggccattt tcattcagtt catcgaccat ttcttgtgca
    14341 tgtttcttta cttctcgcag tgcttcttcc ccatataaat gctgtgatcc tacaacaaac
    14401 caaaattctt tttctccaat attcaacata attagccact ccttttattt gttttgtccg
    14461 taatacgcat tttttccgtg ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc
    14521 tcttctgaaa atgaattgag ctgtctggta aataaattca tcttgcatac ttcatctagt
    14581 acgactgcgt tcattaccgc actttgcgca tctttccccc aagtaaatgg accgtgtccg
    14641 tgtaaaagta cccctggaat tgctaaggga tcaattttgc gttctttgaa tgtttctatg
    14701 ataacttttc ctgtttcata ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga
    14761 gcacaaggta ccgatccata aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc
    14821 aatcctgctt gtgcccagat cgtcgcccat gtcgagtgag tatggacgat tcctccgatt
    14881 tgaggaaatg aacgatataa aacagcatga gtaggcgtat ccgaagaggg attgagttta
    14941 ccttcaatga tatgattgtc caagtccaca acgaccatgt cgtctgctgt catctcttca
    15001 taactgactc cgctaggttt gataacgaaa tatcgtgttt caggatcaaa agcgctcaca
    15061 tttccccatg tatatttgac gagtccgtgt ttaggcaagt caagatttgc ttggtatact
    15121 tcttctttca gttgttccag cattttctat cactcctttt tcttgcaatt gatgaatcgc
    15181 tgttttttca atgtcgatcc cctcacggta acgtctcata aatatctgat agcctgctat
    15241 ttcttctgct gaaggtgtga tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa
    15301 gaagttttct aaagacttcc catgtcgatc catcatatag gctgctaata acgcgattcc
    15361 ccacgctcca ccttctccgg ctgtgtccat cactgtaact ggtgcctcca tagcagatgc
    15421 tagaatccgc tgtccaactt ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc
    15481 aatttggaca ttctcagatt tcaatatatc cattccgatt ctcattgcac caaaagcact
    15541 ggctaaatgg atccgcatga aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa
    15601 taatggcctt ccttcattta ctcctgtaat gttttcaccg gaaaaataac cataagaaag
    15661 taatcccccg caatccgtat cgccttctag cgctttgttg aataataccg tgaataattt
    15721 atctctgctt atctccatac ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc
    15781 attgatatcc gaagagcagt tattcgtatg aaccatgcct actagttctc ctgaaggtgt
    15841 agtgaccatg tcgatttccg gatggacatt ttctagttct ttttctaata cgatcatagc
    15901 gaaggcggaa gtaccagccg aaatgttccc tgtccgtttg gctacactgt tcgtagcgac
    15961 cataccggtt cctgcatccc cttctggcgg acaaaccggg attcctggtc tcaaatttcc
    16021 cgtagggtcg atcagataag cccctgattc gcttaacttg ccagcttctt ctcctgctac
    16081 tagaacttta ggcaaaatgt gatccaagtc aatcgctacg tgcgcttttt cgattaactg
    16141 accaaaaata tttttcattt cttgatggta ggttttcgtt gaaggatcga taggaaacat
    16201 gcctgatgca tctcctacgc ctagtacttt ctcaccagta agctgccaat ggacataacc
    16261 tgcaagcgtt gtcaaaaaat taatttcttt cacatgttct tctttattca aaatagattg
    16321 atacaaatga gcgatactcc atctctgcgg aatattgtat tgaaattttt ccgttaatat
    16381 tcgttctgct tcttcagtga ttgtatttcg ccaagtacga aatggtacta gtaactgttc
    16441 cttgctatca aatgctagat aaccgtgcat cattgcactg aacccaatcg acccgattgt
    16501 cgtaagattc tcgccataga tttcgaatac ttctgctgcc atttttcggt aactgacgcg
    16561 cagtcctttc caaatatctt ctaatgaata ggtccagata ccattttcta gtttgttttc
    16621 ccactcataa ctaccagcgg caataggagt aaaagatgaa tcaatcaata cagctttgat
    16681 cctagttgat ccaaactcaa tccctaaaga tgtacgacca tctatgacat ctaacatact
    16741 ttgcgcattt acttccttca taatgattta actccctcct tctcgtcaaa cctgatcaaa
    16801 ccgtcttttt agtagcgctt acaatgatta ttgtatactt ttgtacgtac atgtcaagcg
    16861 gttatttaca agaatacaat aaaaaacact tgttttgtac gtacataagt tataaaatga
    16921 aattgcttac aaaatctgat caaccgcctt ctcgaaattc tagaaggaga gaatacttat
    16981 gaaaaaagaa aaaaaagttt cctcatcgtt tatttatttt tttggcgcat ttggcggcat
    17041 tctatttgga tatgatattg gtgttatgac cggcgcctta ccttttttac agcatgattg
    17101 gggtttggca ggtaaagctt ctttgatagg atggatcacg tcttcggtca tgttaggtgc
    17161 gatccttggc ggctctttat ctggtcttct ttctgataag cttggacgac gaaaaatgat
    17221 tcttctttct gcacttattt ttatggctgg ttctgtcctt tctgcatctg cccctcacaa
    17281 tggttcttat tttctgattg cagcaaggat tcttctaggt ttagctgttg gcgctgcatc
    17341 tgccctagtt cctgcttata tgtctgaaat ggctcctgct agactaaggg gaagattgtc
    17401 agggatcaat caagtgatga ttgcttctgg catgctcctt tcctatgttg cagattattt
    17461 gctaaaagtg ttgcctgaaa cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc
    17521 cgcgctgatt ttatttttcg gtgttttagc tctaccagag tcaccgcgtt ttcttatgca
    17581 atctggccgc ttggaagagg caaaaagagt attgaactat attcggacac caaaagaagc
    17641 tgaacaggaa ttcgaacaaa tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc
    17701 ttggcatact ttgtttttag aaaaatatcg ttccttagtc attgccggta ttggtgtagc
    17761 agtattccag caatttcaag gagctaatgc tattttttac tatattccgc tgatcgtaga
    17821 aaaagcgact gggcatgcag cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat
    17881 tttagttgct ggttctttgc tctttttggt tattgcagac aagttcaatc ggcgaacttt
    17941 attgaaaata ggcggtagcg tgatgggact ttcattcatc ctaccagctg ttttaggaac
    18001 tgtactggat gctcatacaa attcgttgtt gattctcttg tttttatgta tttatgtcgc
    18061 attttattct tgcacatggg ctccactgac atgggtaatc ataggcgaaa tattcccact
    18121 agctgtccgc gggcgagcat ctggcctagc ctcttctttt aattggattg gttctttcct
    18181 agttggcttg ctttttcctg tgatgactgc ttctatgtct caagaaatcg ttttcggcat
    18241 ttttgggatc atctgctttt taggggtttt atttattcaa gagatcgtac cagaaactcg
    18301 tgggaaatct ttagaggaaa tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat
    18361 ttctatccat cattcttaaa gaggttgtga cagaagtggt cagcttcaag aaataagaag
    18421 gaaccgctta gtttccagtc acaaggaaca atttcaaaaa acagcttctc atgtttgatg
    18481 aatattgtga cttgtgacca gttggtagga gaacaccgtt tagtggagag gtggtgaaag
    18541 aaacgttcag ccttgagcga tgacaaaagc tctcacaaac attgcgacga gtaaccgtag
    18601 gagcaataag aggcggtaaa aacccgtctc ttatttttcg ttttctttca ctgtttcccg
    18661 ttcaactagt acaggttcat agagaatatt tggctgtgcc tgtccttttt caatcgttgt
    18721 gacgatccac cgtgcggcat cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa
    18781 ttttttattc cctaaatggc ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc
    18841 tggaatatga tagccatttt ctgtcaactt atctagcatt aaactagcta tttgatcgtt
    18901 atagcaaatg atgccatcta aagcttgttc ttctttcaac cgttgtactg ttttttcagc
    18961 gattgtttct cgtgtttccg tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt
    19021 atactgttca cacgcgctga taaaaccttt catcctgtat tttccttgaa gatcgtcgat
    19081 ttttgtgatg aacaacagac gctgatgtcc tttttttatc aaatattctg ctgccataaa
    19141 accgactttc acatcatcta aacaaatata agaggcattt aactcttcat aagcagcatt
    19201 gatcatcacc atcggaatgc cttgttctct caaagcaaca taaagtgcta agtttgggtt
    19261 atactgatta cttttcgtag gctcaacgat caaaccatct accccataag caatcatttt
    19321 cttcaaacac tctttctcca attcgtgatc gttattcgta ctggctaaaa gcaatgaata
    19381 cccagattct cgtaattcat tttcgattcc tcggataatc gacggaaaaa tataatcaga
    19441 gagatatgtg gtgatgacac cgatcgtttt tgttctgttc aatggtgttt tttctttctt
    19501 atagctgtca tctacatatg tgcccgaccc tttttcttta cgcagatagc cctcatttac
    19561 caacacagcg attgcttgcc gaatcgtatg tctgctgacg tcatatattt cttgcagctt
    19621 gaattctgag ggaatggttg tatttacttc gtattcctca cgaaggatct tttgttttat
    19681 ctcttctgca atatcttgat attttgccat ttgccttgcc cctttttatt tgtacgtaca
    19741 tatatcataa cgagtctgca aggtttattc aagcaaaaat caaggtttct tgtgctcata
    19801 aggcatacgg aacaggttca ccaaattctg gaaatccttg ctcattccac gtaaagcatt
    19861 gcgcgttcgc gtgccgatta ggattgtcta acggatcccc tttttggttc ttctctggtc
    19921 gagcatgata gatcaaaata tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac
    19981 caggaccaaa ttgcttattt ttttcagaag aaacgaatac tggctccgat aatttatgcc
    20041 atgattcttt taataaaaga ttcttgtgct catcagccca cagcaggccc atggcatagt
    20101 tttcatctgt tgcactacca gaatacgtaa tgaatatttt cccattgcga gtaaggactg
    20161 ctggaccttc atttactgaa aaaccccgtt tttcccattc atactcaggt atagaaagga
    20221 ggatctgctt cccctttaac gtccaaggat tatccatttc ggatagataa agattagaat
    20281 tcccagtaat ttcaggatct tgttgggccc acacataata tagctttcct tgagaagaga
    20341 aagttgttcc gtctaaactg aaagtttccc ttttcgtttt tatttgtccc ttttctatcc
    20401 aatctgtatt cattggatct atatgctgat tttcaagtac gaacatgcgg tgatgatgag
    20461 attcatcacg tatagtttta tgattgcttg cagcaaagta aatataccat tttccattga
    20521 tataatggac ttcaggtgcc cagatcaatt cactcatcgg tccttcttca tgagcctgcc
    20581 atacaatcag gcttgcagca tgttctaatt cattcagtga ctttgccctt cttaattcaa
    20641 tcacttggta gcctggaacc gatcctgtaa aataataata accatctgta tgtttatata
    20701 cccaaggatc tgcccgttgc cgaacgatag gattattata aagtgtcttc atcataacaa
    20761 atttcctcac attttcttta ttctaattac attccaagag tatttttcta tccctacatt
    20821 taattcgtca tgtttttcta ttgaaaaata gtttatttca ggttttattt tttctggttc
    20881 ttcacgtgta tttttgtcca acagatcgct actggttaat gtgtagtgct ctccttgatc
    20941 tagcaaattg aaatcatgtt caatcgttat cttttgttgt tcatttgtgc gattgatcaa
    21001 gaaaatagtc agctgattgc cgttatctac gatcacactg tccacataag gaacattttt
    21061 tatcttttca caatcataaa cagggcaaat gatttgttgt ttcaatactt gacctttagc
    21121 atatttcgat aagtgttgca tcacataata agttggattc ctccatgctt gtcctttagc
    21181 atctgtcagt attaaaggta ttgtgtttac taacaatgat tgacaactaa tctttacgcg
    21241 atctgcgtgt cgtaaaatag ccagccctaa tgaaccaaat aataatgtat cttccatagt
    21301 aaagtaacac caatcgatgg gactaccgac ttcaaaatcc ctatgtcttt tttccggttg
    21361 ggcatgtaca ttccattcat caaacgctag atacattgtt ttctcacttc tcaaatatcc
    21421 ttttacataa tcacaagtag caacgatttc ttcaatttga cgatcgaatc gataagcttt
    21481 tgcaaggtat gtcgctgtat catcacgctc atttttagga ggtttagtca agtcttcttc
    21541 atcatactta tcaatatagt tatgcaaagc taaataatct acattctcat aagcttcttc
    21601 taatgcgata cgatcccatt cagggtacga tggtaaacgt ggcgtagagc ttccaactaa
    21661 gattgtttca attgaatcat ccactaactt catgactttt gaggcttcat ttgctaacct
    21721 accatattca taagcactct tagcccccac ttgccatgga ccgtctagct cattccctaa
    21781 acaccataat ttaacgccaa aaggcttttt ttgcccattt tttattctta aatcactcca
    21841 gaaggttcct tttggaaaat tacaatattc tactaaattt tttgcttcat caattccttt
    21901 tgttcctaaa ttcaccgtga atatcggttt cgcttgtaca tgactgatcc atttaaaaaa
    21961 ttcgtggagt ccaaaagaat ttggttctat acctcgccat gctaaatcaa ttttttttgg
    22021 tcgttgttct ttcctaccta cagtatccac ccaatcataa ccagatgtaa agtttcctcc
    22081 aggatagcga atgacaccta attctaattc ctttacataa gagaggacat cttgtctaaa
    22141 cccgtcacca tcagctgttt catggcccgg ttcataaatc cctgtataga cgacacttcc
    22201 catatgctca acaaatgagc caaacagccg agaactgatc tctcctaccg taaaatcctc
    22261 tctgattttt actttcactt cttcgtccaa cagctttccc tacctttcat ttcctatagt
    22321 caagataata gatacaagtt tccatacgcc accagcaaag ataaaaataa aaggagggaa
    22381 aagaaatatg agcagtacaa atcctaataa caatacgcaa caaagaatat ttctcaaaaa
    22441 atcaaaaaat actttccatg cactttgttc aaaaatttgt aaaaatgtga tatctttgtt
    22501 ttcagaaatc acatatatcc aggataaaag aaacatggaa taaataagta aaactacata
    22561 tccactaatt gaaagtacga ttgtctgttc ttctaataat tgattaacaa gaaaataaat
    22621 aatgatcgtt aacagactaa ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa
    22681 gaaaaaataa atgatatttc ctgatccctc acctttccat tgcgccatta cataaaaaac
    22741 agcacatgta gcaggcacga tagtaataac aggcaaacac acgattaccc agagaatacc
    22801 taataagatg taatcagaaa ccacaatcaa ctgtccataa aatttagtct caactatttt
    22861 catcttcctc actctttcat tccagacata gctgttcctt gaacaaaata tttctgtgca
    22921 aaaatataaa ccactagtaa tggcagcatt gaaataaccg ttccagccat tacaggtcca
    22981 taaaaagaaa tgttctgccc tgtaaataaa gctaaccctg ctggtaaagt tctcattttt
    23041 gaactacttg tcaatatcaa tggataaagc aagtcattcc aactattcat cagcgtgaaa
    23101 atccctaatg ataaaagagt aggtttgctc aatggcaaca tgattttcaa aaaaatatta
    23161 aactcactta atccatcaat gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa
    23221 aaggaacgca tcatgaagat accaaatgca gttgtcatac gaggaatgat caaacctgca
    23281 taagtatcaa gcatgccaag caaattgact tcaataaata gaggaatcat gaagacttgg
    23341 aatgggatca tcattgtcag caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc
    23401 atacgagcaa atgcataccc cgcaagagaa tcaaagaata cagaagtgat cactgtccct
    23461 ccagcaaaaa tcactgtatt tttgatgtaa tctaataaag ggatcgttgt ccaaacctta
    23521 atatagttat ctagggtata gctatctgca aatattctag gcggaaatga gataatgtct
    23581 ttttcgtgtt taaaagaaga aataatcagc cacaaaaaag gaaacgcaat aaccaacaat
    23641 ataactagca tactaatcgt tcgaatgatt tttttaccac taactgcttt tcgactattc
    23701 acagtaactg taatcgagct ttctggtgcc atacaggttt ccctcctatt tcatttgttt
    23761 ttctttccgt gacatgtaca cattagtgga gatagctata gttgcaacaa tgataaacaa
    23821 aaccgaagac agtgctgatg catagcccaa ttcataaggt gctgtaaatc ctcgactgta
    23881 aatatattga acagctgttt ctgtcttaaa ttgtgggcca ccagcggttg caacatatac
    23941 ttggtcaaac acttgcatcg aaccaatcag attcgttaaa atacaaaaac ctaaggaggg
    24001 aacgatctga ggtagcgtaa tgttaaaaaa ctgctgtcga tttgttgcac catccatttg
    24061 agcagattca tacaaagagg gagagattcc ctgaatagca gtcaagagaa tcgtcatagt
    24121 aacaccaaaa ttcttccaaa cagtcatgat ggctaccgtc ggcatagcaa gggttggatc
    24181 gcgaaaaaat tgagggatcg gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac
    24241 attcggatca agcaacatag aaaaaataat cgcaattgac gtcaatgaac aaataactgg
    24301 aatgtaaaat gtcgaacgac aaaaacgatt gaatctggta tttttagata atgctactgc
    24361 tgctaataaa ccaacgataa tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg
    24421 aatcaacgaa ttaactgctc gttcatcact aaatatacgt gtgaaatttt caaaacttac
    24481 aaaatttctt gatgtaaaga aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa
    24541 aagcggtatg acagtaaata ataataaaac taaaaaagat ggagttaaaa acaaatacgc
    24601 agcacgatca ggacgattcc aatatttggt gaatccgttc atatagtcca cctcatcttt
    24661 cattttgata aaatatacaa tcttggttta aacgaatctg aataagatat ccgaacaatc
    24721 gctgtgattt ttgcaataac acctatctgc aaatactagg tcgatcattc agatatctgt
    24781 atcttgccaa cagtctatcc tgtataaagt tctgtactat ttttgcagta attgattgat
    24841 ggcatcatct gcatcgttca tgagtgtctc aggatcttct cctgctaata atttttcaat
    24901 cagtggattg atgatatcgt tattgatcgc agagatttgg gtaaaacctg gtaaatatgg
    24961 ctctgcatag tccatttgtt tcgataattc agatacaata ggatcatttt ttacttcttc
    25021 atcttttgct actgaatcca agtaggcagg aaaaccattt tcgatactcc attttttacc
    25081 aatttcagtt gtattccaat atttcaaaaa ctcatagaca gcttcttttt tattattatc
    25141 tgtacttgcc ggaatggcat atcctacgcc atctaaaata gctgtttttt tgtctgaatc
    25201 actcccttgt ggtaaggtag tcacattata atcaatctcg tttgctttca atccgttatt
    25261 taaccatggc ccattgattt caatggctag ttgaccagca ttcattaaat tgtccatttc
    25321 ggcgccagaa atcgattctg gaccagtctt gtctacatgg atcatttttt ggatcgcttt
    25381 taacgtttta aggttctcag gagacgcaaa ttcagactta gtataatcat cgttcactaa
    25441 tttaccgcca tttgctaaca tccagttata taacggtgca gttccatcct tattgaagac
    25501 aaaacctgat acatttttac tactatcagt taattttggt gccatttctg tcaattcttc
    25561 ccaagtagta ggtggttgtt cagtatctaa tccagcttgt ttaaacaaat cattattcca
    25621 atacatatac atgccttgta cctgcatggg gacaaaatac tgtttacctt ctacttcacc
    25681 taattcaata gccgtttctt taaaatcaga tttatctact ccatcatatt cccagaaatc
    25741 gtctaatggt tgtaccgctc cgttttctac atactgtgca aaatctggat aatttaatgc
    25801 aataaagtca ggtgcttttt tagaagaaat cgctgtagga agtttttcat tcaaattggc
    25861 ccaagtcatg atatccattt caattttgat gtttttatca tttgttttat taaattcatt
    25921 aacgatttct tgcaatattt caccatcaga tgcagtaaac ccattccaaa aagtcaaagt
    25981 cacttcttta tcagaatcag attctgctga acttccgcca ccgcaagctc ctaaaatacc
    26041 tgttgcagct accaacaaag agccaattaa ccatttttta cctttcattt tttgttcctc
    26101 cttcatctga taaacgacat ctgaccacat tccaagatag cggtttcaat aaacaattga
    26161 ttttcccatc ttcagctttt acatttgttg aatgacttgg ttttacttgt tcacttttag
    26221 gaagattagt tgcttttgta tcaaatccac tcatttcaga aaaatcaatc actccctcta
    26281 atgataattc agacaagtga acagttagat tcatttcttc ttctccacgg ttaactgcaa
    26341 atatgacaag ttctttgtca tttattacgg ctatactttc taaatacggc acttcttcaa
    26401 aatcttccgt cgcatagttt gctgaacgaa tttgtgggct taacacctct ccttgtccat
    26461 acacagcagc ttgcataaat ggataataga tcgtctgtct ccaagcttct ccgtttgttt
    26521 ctgtcctgat tggtgcaata acgtttacca attgagcaag acaagcaatc tttactctat
    26581 ctgcatgttt caataaagtg attaatagac agcctaatag taatgcatct tcaaaattat
    26641 agttatcttc tagtaaagga ggagcaatct gccaaggcgc tgcttgctca tcttgttccg
    26701 ctgaatgata ccaaatattc cattcatcaa aggacaggtt gatctgcttc ttactatgtt
    26761 tcttcgcctt gactgtatcg cacatcgctg tcacttcaga aatgaaccga tccatgtcta
    26821 aggaacgtgc aagataatga gaaagatcat ttttttggtt gccataatat tgatgcagag
    26881 acaaataatc tatttgatcg tacgcttgat caagaactgt taactcccaa tcaccaaatg
    26941 taggcatttg ccgattcgaa cttccgcata aaaccacctc aattgaatca tctacgagtt
    27001 tcatcgcctt agccgtttcc tctgctaacc gtccatattc ttccgctgtt ttatgtccaa
    27061 tctgccaagg accgtccatt tcattcccta aacaccatgt cttaattcca aaagggctct
    27121 cttgaccatt cgaacgcctt aaatcactcc aataagttcc tttaggaaaa ttacaatatt
    27181 cgactaaatt acgagctgca tctactccac gagtacccag attgacagcc atattgattt
    27241 cggcccccac atgctgacac cagttataaa attcatgaat cccaacttca ttcgtttcaa
    27301 ttgtccgcca agctaaatcc aaccttctag gtctatttgc tatgggccca atactgtctt
    27361 cccaacgaaa tcctgacaca aaatttccac caggatatct aatcatggga atgttcaatt
    27421 cttttaccaa gtgaatgacg tcttggcgaa atccactttt attagctgat ggatgatctg
    27481 gctgataaat cccttcatat actgctcgtc ccatatgttc aataaatgaa ctgtacaacc
    27541 gcggatcaac tttgcctaca cgcttgacac tactaatcaa cattgtggct tccataactg
    27601 acctccttta atttatacat gaataataca cctcgttgaa agcgttgtca ataatataaa
    27661 tgttaaactt acttattttt tatcgatttt aattaaccaa aatattaata atgattaaaa
    27721 caaataattt aatataactt ttattaacaa atatgttaat atataagaaa aaccttttaa
    27781 gaggtgatat atatgcaact acaaataaac gaagaatcct taccggtata tgaagcatta
    27841 gctagtaaaa caaggatcaa gatcatccag ctactttcca aaaaaaagat gaatgtcaaa
    27901 gatttggcaa aagaacttgg agtgagcagt gctatcacga cgatgcacgt gaaaaagcta
    27961 gaggaagcaa acatcatcaa aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt
    28021 gtcgataaaa tcgatattag ttttccagaa aaaatattca atgcttttga tacgaaagaa
    28081 acatcgatac ctattggtca ctatacaaat tatgcaattg aaccaacatg cggtttagct
    28141 acgatccatg attttatcgg gaaagtggat gaaccacgct attttatgga tcctcgaagg
    28201 atggatgccc gcattctctg gtttaccagc ggatttgtcg agtatcaggc acctaatttt
    28261 ttaaatacag aggatacatt ggagatgttg gaagtttctg tggaaatttc ttctgaattt
    28321 cctttttcaa atgacaattg gccttcagat atcactttca gcctaaatgg tgtggagcta
    28381 ggaacatgga cgagccctgg agattttgct gatatccgcg gtaaatatac gcctgattgg
    28441 tatccagata atcttaatca atacggcttg ctgaagacga tccgaattac caaacacttg
    28501 acgaatatga atggagaacc cttgtcaaac atcacgatca acgacatccc taaagagcaa
    28561 gacacttggc atttgcgaat cgaagtgaaa gacgatgcca aacatgtggg gggctgtact
    28621 ttatttggta aaggattcgg taattacgat caggatatca aattaaaagt ttactacagc
    28681 tgatattttt cttttctatt ttataaaaca aaaacagcca atccctatga agggacggct
    28741 gtttttgctt tattgttatt tccagaaatc atccaaaacg gaaattggca aatgtcgttt
    28801 atgttgtgtc ttgttccacc atgcttcgat tttttcttgt gcttgagcgg gaacttcttt
    28861 tccttctaga taatcatcaa tttcattata tgtgacacct agtgcgactt catctgcaat
    28921 cattggtttc ccatcttcaa gatctgcagt cggaattttt gtgtataatt tttccggtgc
    28981 attcaatgct tgaagcaatt gtttcccttg tcgtttgtcc aaacggaata atggcaaaat
    29041 gtcggctcca ccatcaccaa atttcgtgaa gaatccagta atgttttctg cagcatgatc
    29101 tgtccctaat acggctcctg ctctttctcc agctacggca tattgcgtaa tcatacgttg
    29161 acgtgctttg atatttcctt tgttaaaatc gctgattccc acacctgctt cgcttaatac
    29221 ttgtacttgt gcatcaacag ctggtttgat atttactcgc agactaacat cgggctggat
    29281 aaagtcaaga gcagcttttg catcttcttc atctgcttgt tcaccataag gaagacgaac
    29341 agcgataaat tgatagtttt catcttttgt ttcttcacgc atttcttcca tcgttagttg
    29401 cgccaaacgg cctgctaaag tagaatcttg gccgccgctg atacctagta caaaagtttt
    29461 aagaaacgga tattttttca aatatgcttt cataaagtca atacttttac gtatttcttc
    29521 ttttggatca attgttggtt tgacacctaa ttcattgata atttctgtct gcaggctcat
    29581 ttattctcct cctatgcttc tgtatgaagt tctgctacgc tttttttcat tcgttccatg
    29641 atagccattt tatggttcca gcattcagta gacagatcga ctgggtattt ttgcggattc
    29701 aagtctcgtt tatactcttc ccatagtgaa tctagatttt cttttgtata ttctttgatt
    29761 tcgtttagtg caggtaattc gtaaactctt tttccttcga taaagatatc ttgtaaaact
    29821 gggcgagctg taaagtctcg aacagtttta ttgataaacg tatgcactgg atggaacatg
    29881 aagatagctt cttcttctcg agggtcttca tcccataatg tcacgtagtc tccttcagat
    29941 ttcccatcaa aatttcgagt gatgcgccac acttgttttt tccctggagt agttactttt
    30001 tctgcattgc ttgataactt gatcgtatcc atcatttttc cttcatcatc ttcgatcgat
    30061 actagcttga atactgcacc aagggctggc tgatcatagg ctgtgatcaa ttttgtccca
    30121 acaccccata catcaatttt agctttttgc atctttaaat tcaaaatcgt tgcttcatcc
    30181 aagtcattcg aagcatagat ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga
    30241 acacgcttag atatataggc catatctcca ctgtcgatgc gtacgccttg gaagttgatt
    30301 ttgtcaccta attctttcgc cacgcgaatc gcacttggca cacctgattt caacgtatca
    30361 taagtgtcga ctaagaacac gcagtcttta tgggttttag cgtaggccat aaatgcttca
    30421 taatcatttc cataagattg gactaacgaa tgagcatggg tcccgctagc tggtatccca
    30481 aatatcttcc cagcacgtac attgcttgtt gcatctgctc cgccgatata tgccgctcta
    30541 gttccccaga ctgctgcatc cagttcttgt gcccgtctag ttccaaattc caatagtgga
    30601 tcttctccga tgacagattt gatacgagct gcttttgtcg cgatcaacgt ttggaaattg
    30661 accatattca acagtgctgt ttctactaat tggcagtgcg ctaaaggccc ttccacttgg
    30721 atcaatggtt catttgcaaa aactagttct ccttcacgag cagaacgaac tgttgcttta
    30781 aattcaaaat tttgtaaata ctccaaaaaa ccttcaggat agacttctaa tccacgcaag
    30841 taagcaatat cggaatcatt gaacgtcaga ttctctaaat aattgactaa ccgttccaat
    30901 cctgcaaaaa ccgcgtatcc gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca
    30961 gcatgtaaat cagctcgacc taattcccag tatgtctgca tcatattgat ttgatataga
    31021 tccgtatgca gcgtcaaact gtcgtctggg taagtattat tcatcttgta ttactcctct
    31081 tggcaagttt tcatttccta cccattataa caaaaaaaat taataggaaa agaaatcttc
    31141 cattttttct gaaaaagcac atgttttacg gatatttctt gctaaaaatg aactccatct
    31201 gctaaaacta tcctttctta ttggtattta acctaaacga gaaagaatac ttattttatt
    31261 caatgtatcc gagacacgaa gaaattagcg ttttctattt ttcttacttg ataaaataag
    31321 tagattttcc tactacaatc gtagccgtta agataccgat gattgaacaa atataaccaa
    31381 atgtctctac aacataatca atggcaaaag tttttgaagc aataatacgt ctttcctttt
    31441 ttgcttctta tccccaaaga aacggatata acttattatg tattgaattt ttttggaaag
    31501 gcgaattatg acaagatata ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt
    31561 tttaaaaaga tacaaagtaa gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga
    31621 catcaatcag tctcgccttg ttttt
//
LOCUS       DAZAOG010000015        30491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000015 DAZAOG010000000
VERSION     DAZAOG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.015"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..129)
                     /locus_tag="ACHDLD_000647"
     CDS             complement(<1..129)
                     /locus_tag="ACHDLD_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3362403.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKY"
     gene            complement(131..328)
                     /locus_tag="ACHDLD_000648"
     CDS             complement(131..328)
                     /locus_tag="ACHDLD_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN3362404.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(722..2449)
                     /locus_tag="ACHDLD_000649"
     CDS             complement(722..2449)
                     /locus_tag="ACHDLD_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN3362405.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2442..3635)
                     /locus_tag="ACHDLD_000650"
     CDS             complement(2442..3635)
                     /locus_tag="ACHDLD_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3362406.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3822..4466)
                     /locus_tag="ACHDLD_000651"
     CDS             complement(3822..4466)
                     /locus_tag="ACHDLD_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3362407.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4560..4694)
                     /locus_tag="ACHDLD_000652"
     CDS             complement(4560..4694)
                     /locus_tag="ACHDLD_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN3362408.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4696..6828)
                     /gene="feoB"
                     /locus_tag="ACHDLD_000653"
     CDS             complement(4696..6828)
                     /gene="feoB"
                     /locus_tag="ACHDLD_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN3362409.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6825..7298)
                     /locus_tag="ACHDLD_000654"
     CDS             complement(6825..7298)
                     /locus_tag="ACHDLD_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN3362410.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7699..7923
                     /gene="nrdH"
                     /locus_tag="ACHDLD_000655"
     CDS             7699..7923
                     /gene="nrdH"
                     /locus_tag="ACHDLD_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN3362411.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8082..10241
                     /gene="nrdE"
                     /locus_tag="ACHDLD_000656"
     CDS             8082..10241
                     /gene="nrdE"
                     /locus_tag="ACHDLD_000656"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN3362412.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10291..11256
                     /gene="nrdF"
                     /locus_tag="ACHDLD_000657"
     CDS             10291..11256
                     /gene="nrdF"
                     /locus_tag="ACHDLD_000657"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN3362413.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11345..12484)
                     /locus_tag="ACHDLD_000658"
     CDS             complement(11345..12484)
                     /locus_tag="ACHDLD_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3362414.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12793..13506)
                     /locus_tag="ACHDLD_000659"
     CDS             complement(12793..13506)
                     /locus_tag="ACHDLD_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN3362415.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13760..14461)
                     /gene="nagB"
                     /locus_tag="ACHDLD_000660"
     CDS             complement(13760..14461)
                     /gene="nagB"
                     /locus_tag="ACHDLD_000660"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN3362416.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14695..16227
                     /locus_tag="ACHDLD_000661"
     CDS             14695..16227
                     /locus_tag="ACHDLD_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3362417.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16418..17620)
                     /locus_tag="ACHDLD_000662"
     CDS             complement(16418..17620)
                     /locus_tag="ACHDLD_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3362418.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17702..18391)
                     /locus_tag="ACHDLD_000663"
     CDS             complement(17702..18391)
                     /locus_tag="ACHDLD_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3362419.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18497..18865)
                     /gene="rplL"
                     /locus_tag="ACHDLD_000664"
     CDS             complement(18497..18865)
                     /gene="rplL"
                     /locus_tag="ACHDLD_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN3362420.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18918..19421)
                     /gene="rplJ"
                     /locus_tag="ACHDLD_000665"
     CDS             complement(18918..19421)
                     /gene="rplJ"
                     /locus_tag="ACHDLD_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN3362421.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19625..19752)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19831..20520)
                     /gene="rplA"
                     /locus_tag="ACHDLD_000666"
     CDS             complement(19831..20520)
                     /gene="rplA"
                     /locus_tag="ACHDLD_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN3362422.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20624..21046)
                     /gene="rplK"
                     /locus_tag="ACHDLD_000667"
     CDS             complement(20624..21046)
                     /gene="rplK"
                     /locus_tag="ACHDLD_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN3362423.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21389..22057
                     /gene="sdaAB"
                     /locus_tag="ACHDLD_000668"
     CDS             21389..22057
                     /gene="sdaAB"
                     /locus_tag="ACHDLD_000668"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN3362424.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22070..22942
                     /gene="sdaAA"
                     /locus_tag="ACHDLD_000669"
     CDS             22070..22942
                     /gene="sdaAA"
                     /locus_tag="ACHDLD_000669"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN3362425.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23033..24133)
                     /locus_tag="ACHDLD_000670"
                     /pseudo
     CDS             complement(23033..24133)
                     /locus_tag="ACHDLD_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24225..25112)
                     /locus_tag="ACHDLD_000671"
     CDS             complement(24225..25112)
                     /locus_tag="ACHDLD_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3362426.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25189..25734)
                     /gene="nusG"
                     /locus_tag="ACHDLD_000672"
     CDS             complement(25189..25734)
                     /gene="nusG"
                     /locus_tag="ACHDLD_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN3362427.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25835..26005)
                     /gene="secE"
                     /locus_tag="ACHDLD_000673"
     CDS             complement(25835..26005)
                     /gene="secE"
                     /locus_tag="ACHDLD_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN3362428.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26029..26181)
                     /gene="rpmG"
                     /locus_tag="ACHDLD_000674"
     CDS             complement(26029..26181)
                     /gene="rpmG"
                     /locus_tag="ACHDLD_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3362429.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26343..26987
                     /gene="cbpA"
                     /locus_tag="ACHDLD_000675"
     CDS             26343..26987
                     /gene="cbpA"
                     /locus_tag="ACHDLD_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN3362430.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27043..27546)
                     /locus_tag="ACHDLD_000676"
     CDS             complement(27043..27546)
                     /locus_tag="ACHDLD_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN3362431.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27559..27605)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28143..29108
                     /locus_tag="ACHDLD_000677"
     CDS             28143..29108
                     /locus_tag="ACHDLD_000677"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3362432.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29207..30379)
                     /locus_tag="ACHDLD_000678"
     CDS             complement(29207..30379)
                     /locus_tag="ACHDLD_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362433.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30366..>30491)
                     /locus_tag="ACHDLD_000679"
     CDS             complement(30366..>30491)
                     /locus_tag="ACHDLD_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362434.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9301 a         6263 c         5401 g         9526 t
ORIGIN      
        1 atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata
       61 tttaggatct ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt
      121 ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc
      181 ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca
      241 ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt
      301 taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga
      361 ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat
      421 aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat tctttaatat
      481 tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg
      541 atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttggacgtt
      601 tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga
      661 gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt
      721 tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt
      781 gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc
      841 aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa
      901 ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca
      961 gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt
     1021 ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc
     1081 agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc
     1141 ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta
     1201 catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt
     1261 cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg
     1321 aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg
     1381 gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag
     1441 caacttctgt atcccattca acataagata agttgatacg ttttgtttct ttggcagatg
     1501 aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc
     1561 cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt
     1621 tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga
     1681 caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg
     1741 gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga
     1801 tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac
     1861 ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta
     1921 aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa
     1981 ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct
     2041 gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga
     2101 tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag
     2161 tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga
     2221 acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga
     2281 taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc
     2341 caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat
     2401 tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc
     2461 tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt
     2521 ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt
     2581 accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt
     2641 ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt
     2701 ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt
     2761 ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc
     2821 actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc
     2881 aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg
     2941 tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca
     3001 ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc
     3061 gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca
     3121 acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa
     3181 ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg
     3241 acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt
     3301 tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc
     3361 gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat
     3421 agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct
     3481 tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc
     3541 tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat
     3601 atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat
     3661 tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca
     3721 aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa
     3781 aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc
     3841 tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg
     3901 atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga
     3961 gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg
     4021 tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta
     4081 tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca
     4141 tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca
     4201 tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt
     4261 ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc
     4321 tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact
     4381 ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg
     4441 cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat
     4501 cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat
     4561 catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta
     4621 ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat
     4681 aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt
     4741 ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata
     4801 cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact
     4861 aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt
     4921 catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc
     4981 taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct
     5041 gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc
     5101 tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg
     5161 ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac
     5221 aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat
     5281 aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc
     5341 tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc
     5401 aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa
     5461 ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat
     5521 aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc
     5581 ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat
     5641 cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac
     5701 aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat
     5761 aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat
     5821 cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg
     5881 taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg
     5941 aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg
     6001 attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac
     6061 aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc
     6121 actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg
     6181 agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc
     6241 ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc
     6301 ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc
     6361 ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac
     6421 tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat
     6481 gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt
     6541 taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg
     6601 ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata
     6661 gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt
     6721 ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa
     6781 caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc
     6841 ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact
     6901 gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc
     6961 gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg
     7021 atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc
     7081 tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt
     7141 accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg
     7201 atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga
     7261 taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat
     7321 aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa
     7381 atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga
     7441 tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag
     7501 ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc
     7561 tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt
     7621 ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa taagaatgtg
     7681 ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt
     7741 aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat
     7801 gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt
     7861 acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc
     7921 taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca
     7981 gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca
     8041 atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac
     8101 aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc
     8161 ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata
     8221 cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag
     8281 aagcagaatt cattcaattg tattcaccat catttattga aaagctatat caatttttgg
     8341 acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg
     8401 cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca
     8461 atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga
     8521 tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg
     8581 gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct
     8641 caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa
     8701 atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc
     8761 cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag
     8821 gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa
     8881 aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac
     8941 ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata
     9001 gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc
     9061 atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg
     9121 aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga
     9181 accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac
     9241 aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta ggaacggata
     9301 tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc
     9361 aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg
     9421 tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg
     9481 gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact
     9541 ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg
     9601 ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt
     9661 atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt
     9721 ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga
     9781 aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata
     9841 tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga
     9901 agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt
     9961 acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca atggcacaac
    10021 aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca
    10081 tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt
    10141 acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag
    10201 aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct
    10261 tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac
    10321 tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt
    10381 tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa
    10441 ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa
    10501 tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt
    10561 ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt
    10621 acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat
    10681 ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag
    10741 aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt
    10801 tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga
    10861 gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta
    10921 cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac
    10981 gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc
    11041 aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc
    11101 tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca
    11161 agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg
    11221 atgaaagacg acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt
    11281 ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt
    11341 atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt
    11401 ccattcttct caataaatag attttccgga tatttaatat tatcgtctta atcactgcat
    11461 agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata
    11521 ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca
    11581 aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg
    11641 gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg
    11701 ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata
    11761 ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga
    11821 tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg
    11881 ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata
    11941 gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt
    12001 ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat
    12061 ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt
    12121 catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg
    12181 tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa atgattacga
    12241 ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa
    12301 cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa aaaactgccg
    12361 aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga
    12421 tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt
    12481 gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg
    12541 cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga
    12601 cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt
    12661 ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa
    12721 tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca
    12781 acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac
    12841 ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat
    12901 atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac
    12961 gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa
    13021 ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc
    13081 aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat
    13141 ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt
    13201 cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag
    13261 ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa
    13321 tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa
    13381 aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc
    13441 gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc
    13501 attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt
    13561 gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta
    13621 tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg
    13681 ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    13741 tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa
    13801 cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca
    13861 tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt
    13921 tcataattga accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga
    13981 agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt
    14041 caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt
    14101 ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat
    14161 cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga
    14221 catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta
    14281 agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag
    14341 taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt
    14401 tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca
    14461 tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt tttcttttta
    14521 caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc
    14581 gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag
    14641 tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt
    14701 atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc
    14761 gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa
    14821 agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg
    14881 caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca
    14941 atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct
    15001 tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa
    15061 ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg
    15121 agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt
    15181 ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc
    15241 ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt
    15301 caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc
    15361 accacccata tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa
    15421 caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa
    15481 caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca
    15541 acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct
    15601 tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg
    15661 gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc
    15721 atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta
    15781 ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac
    15841 acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga aacaccacta
    15901 caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct
    15961 aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa
    16021 caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat
    16081 gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa
    16141 tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa
    16201 cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg
    16261 tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt
    16321 cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt
    16381 acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc
    16441 tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc
    16501 taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag
    16561 ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg cttgctgctt
    16621 cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc ccgacgtcta
    16681 ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat aaagcaataa
    16741 agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa taagaatctg
    16801 ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc gctaatatcc
    16861 ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc
    16921 aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct cttaatagac
    16981 gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa acagctgtac
    17041 tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga attccgactg
    17101 cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca ataccagata
    17161 ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc atgaccttcc
    17221 ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg
    17281 aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag
    17341 cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt ggacgatcca
    17401 ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat tgggtcaaca
    17461 tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact tttgttccaa
    17521 tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat aatagtaaaa
    17581 taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc catttcctat
    17641 cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt cgccacactt
    17701 attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg aagacaacta
    17761 accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta tatccgtgaa
    17821 acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca atcacgatcg
    17881 acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg accatagaag
    17941 atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt ttcatttgtc
    18001 ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca
    18061 taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg atcgcacctg
    18121 tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga atacctggaa
    18181 cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc ccatgcgaac
    18241 gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat tggatgtctg
    18301 tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt cccaacgaga
    18361 tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat ctatattcat
    18421 taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc
    18481 ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt
    18541 tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa
    18601 ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt
    18661 taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag
    18721 cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt
    18781 cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa
    18841 cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt
    18901 aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca
    18961 gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct
    19021 tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg
    19081 atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc
    19141 gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg
    19201 tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact
    19261 ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt
    19321 aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca
    19381 tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg
    19441 aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct
    19501 caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg
    19561 aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca
    19621 tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat
    19681 tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag
    19741 ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc
    19801 agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc
    19861 aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg
    19921 tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc
    19981 aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac
    20041 tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt
    20101 agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat
    20161 catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac
    20221 catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc
    20281 tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc
    20341 acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt
    20401 tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata
    20461 aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat
    20521 tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc
    20581 aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc
    20641 ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta
    20701 ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt
    20761 tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag
    20821 cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa
    20881 ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca
    20941 tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac
    21001 ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct
    21061 taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct
    21121 gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta
    21181 tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt
    21241 tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta
    21301 gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat
    21361 tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga
    21421 tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat
    21481 cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata tttatctata
    21541 tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct
    21601 gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc
    21661 cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa
    21721 gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg
    21781 caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac
    21841 cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc
    21901 tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc
    21961 cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca
    22021 gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc
    22081 gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc
    22141 agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa
    22201 cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg
    22261 aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag
    22321 tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa
    22381 aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct
    22441 gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac
    22501 cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg
    22561 atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa
    22621 aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg
    22681 actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt
    22741 aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat
    22801 tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt
    22861 caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga
    22921 gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat
    22981 cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca
    23041 tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg
    23101 acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca
    23161 acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga
    23221 agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta
    23281 cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt tgacgtccac
    23341 catgattgga tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg
    23401 gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt
    23461 catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc
    23521 cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt
    23581 ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa
    23641 tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt
    23701 ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt
    23761 tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt
    23821 tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga
    23881 taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc
    23941 cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg
    24001 tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt
    24061 ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg
    24121 tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa
    24181 cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata
    24241 aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt
    24301 tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc
    24361 gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca
    24421 gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga
    24481 tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta
    24541 tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat
    24601 gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc
    24661 cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg
    24721 atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc
    24781 ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact
    24841 gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta
    24901 aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct
    24961 atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc
    25021 gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca
    25081 cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa
    25141 gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt
    25201 atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac
    25261 tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc
    25321 gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct
    25381 cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct
    25441 tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca
    25501 tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat
    25561 ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa
    25621 aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt
    25681 ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg
    25741 ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc
    25801 attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc
    25861 tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga
    25921 ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat
    25981 cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc
    26041 tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc
    26101 gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag
    26161 tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc
    26221 ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg
    26281 gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag
    26341 cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta
    26401 gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct
    26461 tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata
    26521 aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt
    26581 tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata
    26641 ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta
    26701 atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca
    26761 caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg
    26821 ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc
    26881 taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga
    26941 attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa
    27001 aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa
    27061 atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc
    27121 acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat
    27181 ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt
    27241 tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc
    27301 gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga cgatgacccc
    27361 gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga
    27421 aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata
    27481 aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt
    27541 ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg
    27601 gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct
    27661 gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg
    27721 tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc
    27781 aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc
    27841 tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg
    27901 aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt
    27961 cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa
    28021 cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta
    28081 ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa
    28141 tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga aatggctata
    28201 aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt
    28261 atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga
    28321 ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag
    28381 gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc
    28441 cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca ggccttccaa
    28501 aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca
    28561 taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta
    28621 taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga
    28681 acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc
    28741 aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat
    28801 ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg
    28861 ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa
    28921 tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc
    28981 actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg
    29041 gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt
    29101 ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa
    29161 aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc
    29221 aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca
    29281 ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat
    29341 tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga
    29401 atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga
    29461 taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta
    29521 ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta
    29581 aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa
    29641 gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat
    29701 tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa
    29761 atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga
    29821 aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct
    29881 caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt
    29941 tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa
    30001 ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc tttcctactt
    30061 cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa
    30121 gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat
    30181 tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt
    30241 tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa
    30301 caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa
    30361 tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa
    30421 aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag
    30481 aaataaagtt t
//
LOCUS       DAZAOG010000016        27866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000016 DAZAOG010000000
VERSION     DAZAOG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.016"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..3663
                     /gene="rpoB"
                     /locus_tag="ACHDLD_000680"
     CDS             46..3663
                     /gene="rpoB"
                     /locus_tag="ACHDLD_000680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN3362435.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            3742..7395
                     /gene="rpoC"
                     /locus_tag="ACHDLD_000681"
     CDS             3742..7395
                     /gene="rpoC"
                     /locus_tag="ACHDLD_000681"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN3362436.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(7990..9015)
                     /locus_tag="ACHDLD_000682"
     CDS             complement(7990..9015)
                     /locus_tag="ACHDLD_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362437.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            9290..10975
                     /locus_tag="ACHDLD_000683"
     CDS             9290..10975
                     /locus_tag="ACHDLD_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN3362438.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            10989..12272
                     /locus_tag="ACHDLD_000684"
     CDS             10989..12272
                     /locus_tag="ACHDLD_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3362439.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            12342..13211
                     /locus_tag="ACHDLD_000685"
     CDS             12342..13211
                     /locus_tag="ACHDLD_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362440.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            13208..14044
                     /locus_tag="ACHDLD_000686"
     CDS             13208..14044
                     /locus_tag="ACHDLD_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362441.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            14070..15137
                     /locus_tag="ACHDLD_000687"
     CDS             14070..15137
                     /locus_tag="ACHDLD_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN3362442.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            15134..15961
                     /locus_tag="ACHDLD_000688"
     CDS             15134..15961
                     /locus_tag="ACHDLD_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3362443.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            16006..17031
                     /locus_tag="ACHDLD_000689"
     CDS             16006..17031
                     /locus_tag="ACHDLD_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3362444.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            17028..19211
                     /locus_tag="ACHDLD_000690"
     CDS             17028..19211
                     /locus_tag="ACHDLD_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN3362445.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            19211..20341
                     /locus_tag="ACHDLD_000691"
     CDS             19211..20341
                     /locus_tag="ACHDLD_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3362446.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            20338..21015
                     /gene="pgmB"
                     /locus_tag="ACHDLD_000692"
     CDS             20338..21015
                     /gene="pgmB"
                     /locus_tag="ACHDLD_000692"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN3362447.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            21099..21626
                     /gene="msrA"
                     /locus_tag="ACHDLD_000693"
     CDS             21099..21626
                     /gene="msrA"
                     /locus_tag="ACHDLD_000693"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3362448.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(21827..22513)
                     /locus_tag="ACHDLD_000694"
     CDS             complement(21827..22513)
                     /locus_tag="ACHDLD_000694"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN3362449.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            22593..24131
                     /locus_tag="ACHDLD_000695"
     CDS             22593..24131
                     /locus_tag="ACHDLD_000695"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN3362450.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24251..24718
                     /locus_tag="ACHDLD_000696"
     CDS             24251..24718
                     /locus_tag="ACHDLD_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN3362451.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            25252..25680
                     /gene="rplM"
                     /locus_tag="ACHDLD_000697"
     CDS             25252..25680
                     /gene="rplM"
                     /locus_tag="ACHDLD_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN3362452.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            25696..26088
                     /gene="rpsI"
                     /locus_tag="ACHDLD_000698"
     CDS             25696..26088
                     /gene="rpsI"
                     /locus_tag="ACHDLD_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN3362453.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(26176..27402)
                     /locus_tag="ACHDLD_000699"
     CDS             complement(26176..27402)
                     /locus_tag="ACHDLD_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3362454.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(27474..27776)
                     /locus_tag="ACHDLD_000700"
     CDS             complement(27474..27776)
                     /locus_tag="ACHDLD_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362455.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         8951 a         5175 c         5963 g         7777 t
ORIGIN      
        1 tgatcgggta gtctttttga atgacatttt agaggggtga agagcttggc tggacacgta
       61 gtaaaatacg gaaaacatcg cgaacgtaga agtttcgcaa gaatcagtga agtgttggaa
      121 ttaccaaatt tgatcgaaat tcaaacagac tcttaccaat ggttcttaga tgagggactt
      181 agagaaatgt ttgaagacat tttgccaatc gatgacttca atggcaatct atctttggaa
      241 tttgtagatt atgaattgaa agaaccaaag tatacagtag cagaagctcg tgcgcatgat
      301 gcaaactact ctgcaccatt acatgtaaca ttgcgtttga caaaccgcga gactggcgaa
      361 atcaaagcac aagaagtatt cttcggtgat ttcccattaa tgacagaaca aggaacattc
      421 atcatcaacg gggcagagcg agtaatcgtt tctcagttag tccgttcacc aggtgtctat
      481 ttccatggaa aagtcgacaa aaacggaaaa gaaggattcg gttctactgt tatccctaac
      541 cgtggagctt ggctagaaat ggaaactgat gcaaaagaca tctcttatgt acgtatcgac
      601 cgtacacgta agattccttt aaccgtatta gttcgtgcgt taggatttgg ttccgatgat
      661 acgatctttg aaatcttcgg cgacagcgaa acattacgca atacagtcga aaaagacttg
      721 cataaaaacg caagcgactc aagaacagaa gaaggtttga aagacgttta cgaacgtttg
      781 cgtccaggcg aaccaaaaac agcagacagc tcacgtaact tgctgaatgc tcgtttcttc
      841 gatccaaaac gttacgactt agcaaacgtt ggtcgttaca aagtaaacaa aaaattagat
      901 ttgaaaacac gtctattgaa tctgacatta gctgaaacat tagttgatcc agaaacaggc
      961 gagatcatcg ttgaaaaagg aacagttctg actcaccaag tcatggaaac actagctcct
     1021 ttcattgata atggcctaaa ttcagtgact tactatccat cagaagacgg tgtggtcaca
     1081 gatccaatga ctgtccaagt catcaaagta ttttcaccaa gagaccctga acgtgaagta
     1141 aacgtgatcg gaaacggcta tccagaagca gctgtaaaaa cggttcgtcc agccgacatc
     1201 attgcttcaa tgagctattt cttgaacttg atggaaggta tcggcaatgt cgatgatatc
     1261 gaccacttag gaaatcgtcg tatccgttca gtcggtgaac ttttacaaaa ccaattccgt
     1321 atcgggctag cacgtatgga acgtgtggtt cgtgaaagaa tgtcgatcca agatactgaa
     1381 acattgacac ctcaacaatt gatcaatatc cgtccagttg tggcaagtat caaagaattc
     1441 tttggttctt ctcagttgtc acagttcatg gatcaaacaa accctcttgg cgaattgaca
     1501 cacaaacgtc gtctttcagc cttaggacct ggcggattga ctcgtgaccg cgccggttat
     1561 gaagttcgtg acgttcacta ctcccactat ggccgtatgt gtccgatcga aactcctgaa
     1621 ggcccaaaca tcgggttgat caatagtttg tccagttatg cgaaagtaaa taaattcgga
     1681 tttatcgaaa caccttatcg tcgtgtagac cgtgaaacag gtcgtgttac tgaccaaatc
     1741 gactacttga cagctgatat cgaagaccat tacatcgtag cccaagcaaa cagcccattg
     1801 aatgaagatg gaacatttgc tcaagacgtt gttatggctc gtgctcaaag tgaaaactta
     1861 gaagtatcta ttgataaagt agactacatg gacgtatcac ctaaacaagt agttgccgtt
     1921 gcgacagcat gtattccttt cttggaaaac gatgactcca accgtgcctt gatgggtgcc
     1981 aacatgcagc gtcaagcagt gccgttgatc aatcctcaag caccatgggt tggtactggt
     2041 atggaataca aatcagcgca tgactcagga gctgctttgt tatgtaaaca tgacggtgtc
     2101 gttgaatacg ttgatgcttc tgaaatccgc gtacgccgcg acaacggtgc attagataaa
     2161 tacgatgtaa caaaattccg tcgttcaaac tccggaacaa gctacaacca acgtccaatc
     2221 gttcatttgg gtgagaaagt cgaaaaaggg gatactctgg cagacggacc atctatggaa
     2281 caaggcgaaa tggctctagg ccaaaacgtt ttagttggtt tcatgacttg ggaagggtac
     2341 aactatgaag atgcgatcat catgagccgt cgtttggtaa aagatgacgt atacacttct
     2401 atccatattg aagaatatga atcagaagcc cgcgatacaa aattaggacc tgaagaaatc
     2461 acacgcgaaa tccctaatgt cggggaagac gcattaaaag atttagacga aatgggtatc
     2521 atccgtattg gtgcagaagt ccaagatggc gatttgcttg tagggaaagt cacgccaaaa
     2581 ggggtaacag aattatctgc agaagaacgt ctattgcatg cgatctttgg cgaaaaagct
     2641 cgtgaagtcc gcgatacatc attacgtgta ccacatggcg gcggcggtat cgttcacgat
     2701 gtgaaaatct ttactcgtga agctggagat gaattgtcac caggcgtaaa catgttagtt
     2761 cgtgtttata tcgttcaaaa acgtaaaatc catgaagggg ataaaatggc cggtcgtcac
     2821 ggaaataaag gggttgtatc ccgtattatg ccggaagaag atatgccatt cctacctgat
     2881 ggaacaccaa tcgatatcat gctgaatcct ttaggggtgc cttcacgtat gaacatcggg
     2941 caagtattgg aattacactt gggtatggct gcacgtcagt taggtatcca cgtagcaacg
     3001 ccagtatttg atggtgcaag tgatgaagac gtatgggaaa cagttcgtga agccggtatg
     3061 gccagcgatg cgaaaactgt tctgtacgac ggacgtacag gtgaaccatt tgatggacgt
     3121 gtgtctgtcg gtgtgatgta catgatcaaa cttgctcaca tggtcgatga caaattgcat
     3181 gcacgttcaa tcggacctta ctcattggtt acacaacaac cattgggagg taaagctcaa
     3241 tttggtggac aacgttttgg tgaaatggaa gtatgggcac tggaagctta cggtgcggct
     3301 tatactttac aagaaatctt gacatacaaa tcagatgacg tagtcggacg tgtgaaaaca
     3361 tacgaagcaa tcgtcaaggg tgaaccaatt ccaaaaccag gtgtaccgga atcattccgc
     3421 gtattggtga aagaattgca atcactaggt ctggacatgc gtgtcttgga tatcgaagaa
     3481 acagagatcg aacttcgcga catggacgat gaagacgacg atttgatcac tgttgatgct
     3541 ttgacaaaat ttgccgaaca gcaaactgcc aaagaattag aaaagaaagc tgctgaacaa
     3601 gtggaagatg aaaaagacga tgtcatccaa aactttgaaa ccgcagaaga taatttagac
     3661 taatccggtc tatgagctgc aaggcggtcg aagatcagcc gtcttgcggt ttgccattaa
     3721 atgaaatgga gggaacccct tttgatcgat gtaaataaat tcgaaagtat gcaaataggt
     3781 ttggcttctc ccgaaaaaat cagaagctgg tcttacggtg aagtaaaaaa acctgagacg
     3841 attaactatc gtaccttaaa acctgaacgt gaagggttgt tctgtgagcg gatttttggt
     3901 cctacaaaag actgggaatg tgcctgcgga aaatacaaac gtatccgtta taaaggaatc
     3961 gtttgtgatc gatgcggagt ggaagttact cgttcgaaag ttcgccgtga acgcatggga
     4021 catattgaat tagcagctcc tgtttcacat atctggtact ttaaagggat tccttcacgt
     4081 atgggtcttg tattagacat gagcccacgc gctttggaag aaatcattta ttttgcttca
     4141 tatgtagtca tcgaaccagg aaatacgaat ttagagaaaa aacaattgtt gacagaacgc
     4201 gaatatcgcg aaaaacgtga acaatatgga caagaattcc aagctgctat gggtgcagaa
     4261 gcgattaaac aattacttga taatgttgat ctagatgcag aagcagccga attgaaagaa
     4321 gaattgaaat ctgcacaagg acaaaaacgt acacgtgcaa tccgccgttt ggatatttta
     4381 gaagcattcc gcgcttcagg aaatcaacca agctggatgg ttatggacgt tatccctgtt
     4441 attccaccag atttacgccc aatggttcaa ttagaaggtg gacgttttgc aacaagtgac
     4501 ttgaatgatc tttaccgtcg tgtaatcaac cgtaataacc gtttgaaacg tttgttagac
     4561 ttgaatgcac ctggaatcat cgttcaaaat gaaaaacgta tgctgcaaga agcagtagat
     4621 gcattgatcg ataacggtcg tcgcggacgt ccagttactg gaccaggtaa ccgcccattg
     4681 aaatctcttt ctcatatgtt gaaagggaaa caaggtcgtt tccgtcaaaa cttgctaggt
     4741 aaacgtgtcg actactcagg tcgttccgtt atcgttgttg gacctttctt gaagatgtac
     4801 caatgtggac ttccaaaaga aatggccatt gaattattca aaccattcgt gatgcgtgag
     4861 cttgttcaac gcgaactagc ttcaaacatc aaaaatgcga aacgtaaaat cgaacgtcaa
     4921 gaagacgaag tatgggatgt tttagaagac gtaatcaagg aacacccagt attgctgaac
     4981 cgggcaccta cgcttcaccg acttggtatc caagcattcg aaccagtttt agtacaagga
     5041 cgcgcaatcc gtttgcatcc acttgtatgt gaagcttata atgccgattt cgatggagac
     5101 caaatggctg tccacgttcc attgaatgaa gaagcacaag cagaagcacg tatgttgatg
     5161 ttagctgctc aaaacatctt gaaccctaaa gatggtaaac cagttgttac tccttctcaa
     5221 gatatggttc taggtaacta ctacttgaca atggaagaag aaggacgcga aggcgaagga
     5281 atggtcttca gagatcaaaa cgaagccgtt atcgcatggc gcaacggata tgttcattta
     5341 cattcacgta tcggtgtgaa ccctaactca ttaggtgaaa aaccatttac tgaatggcaa
     5401 aaagaacgta cgatgatcac aacagttggt aagatcatct tcaacgagat catgcctcct
     5461 gaattccctt atctgaatga accaacagac ttcaacttga ccgtccaaac accggacaaa
     5521 tactttgttg aagctggtac tgatattcct gcacatatca aagagcaaga attagtatta
     5581 ccgttcaaga agaaaaactt aggaaacatc atcgcagaag tcttcaaacg attcaaagta
     5641 acagaaactt ctaagatgct tgaccgtatg aaagacttag ggtacaaaca ttcgacacat
     5701 gccggtatca cagtagggat cgctgacatc agtgtactga atgaaaaaca agaaatcatt
     5761 gaaaatgccc ataaacaagt tgaaactatt acaaaacaat tccgtcgtgg tctgatcaca
     5821 gatgacgaac gttatgaacg tgtcattgct gtttggaatg ctgcaaaaga cagcatccaa
     5881 caaaaactga tggaaggttt ggaagcgaaa aatccaatct tcatgatgtc tgattctgga
     5941 gctcgtggta acatttccaa ctttacacag cttgccggaa tgcgtggttt gatggccgct
     6001 cctaatggtc gaatcatgga attgccaatc atttcgaact tccgtgaagg actatccgtc
     6061 ttggaaatgt ttatctcaac tcacggggct cgtaaaggga tgaccgatac agccttgaag
     6121 actgccgatt caggttactt gactcgtcgt ctggttgacg tggcccaaga tgtaatcatc
     6181 cgtgaagaag attgcggtac tgaccgaggt cttgaaatcc aagccatccg cgagggaaat
     6241 gaaatcatcg aatcattaga agatcgttta gtgggacgtt acacccgtaa agaagtacgt
     6301 catcctgaaa caaatgaatt gatcattgca ggaaaccaat tgatttccga agacatcgca
     6361 aaacaaatcg ttgatgcagg tgtcgaaaca gtgacaatcc gttctgtctt cacatgtaat
     6421 acaaaacatg gtgtctgcaa acattgttac ggacgcaacc ttgcaactgg ctctgacgtt
     6481 gaagtcggag aagcagttgg tacgatcgcc gctcaatcaa tcggtgaacc tggtactcag
     6541 ttgacgatgc gtacattcca tacaggtggg gttgccggag acgatatcac tcaaggtctt
     6601 cctcgtgtcc aagaaatctt tgaagcacgt aatccgaaag ggcaagcagt gatcacagaa
     6661 gtaacagggg atgtcatcga tatctcagaa gatcctgcaa cacgtaccaa agaagtaaca
     6721 atcaaaggaa aaacagatac acgtacgtat actgttcctt atacagcacg tatgaaagta
     6781 gcagaaggcg atatgatcca ccgtggtgca ccattgacag aaggatcaat cgatccaaaa
     6841 caattgttgc aagttcgcga tgtattatct gttgaaaact acttgcttcg tgaagtacaa
     6901 cgtgtttacc gtatgcaagg ggtagaaatc ggcgacaagc atatcgaagt aatggttcgt
     6961 caaatgctgc gtaaagttcg tgtaatggat ccaggtgata cagatatctt accaggtaca
     7021 ttattagaca tcgcagactt taaagatcaa aactacaata cgctagtctc aggaggcgtt
     7081 cctgcaactt ctcgacctgt cttactaggt atcacaaaag catcattaga aacaaatagt
     7141 ttcttatctg ctgcatcatt ccaagaaaca actcgtgtct tgacggatgc tgcgatccgc
     7201 ggtaagaaag atcctcttct tggtttgaaa gaaaatgtta ttataggtaa gatcatccca
     7261 gctggtactg gtatgcaacg ttatcgtgac atggaaccaa aagaagtcgg tgtagcaagc
     7321 gaaaatgtct acagtatttc agatattgaa gcgcaaatgg ctgcagaaga cgcaatgaac
     7381 aatcaaacaa aatagatatt ttaaaaaaga ggttgtgaca aattattgtc acaacctctt
     7441 ttttggtttg tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa
     7501 catcggttcg tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac
     7561 aaaagtaaag atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt
     7621 atttttaata ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga
     7681 catgtctgtt attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa
     7741 taagggtaaa tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa
     7801 tagttttttc ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct
     7861 atctaaaaca gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg
     7921 atgagtgatt gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat
     7981 aattctgttc tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag
     8041 aatgattctt ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc
     8101 tttgattcct aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga
     8161 ggacaataag ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga
     8221 ttgttctgcc actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt
     8281 tggtctttct cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa
     8341 gccgccgtct ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg
     8401 gtaaccattg agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat
     8461 atgacggtgt ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc
     8521 cgtgtccact gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg
     8581 aatattatat tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat
     8641 aattcctcgg atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg
     8701 tgtttttgcc gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga
     8761 ttgaataaca tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa
     8821 tgctagaatc gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata
     8881 attcaattct ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg
     8941 gttatttaat actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata
     9001 aatagtaggt ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat
     9061 agtacaatag acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat
     9121 catgagagac catatttttt taagaagcta ttaattattg atacataagc tttcttattt
     9181 gtatttttgt gtatatcaaa attatttaaa aaaatgctat tgacagtaag cgttttccat
     9241 aatatgatca aatcgttggg aacgctccca aaaaagaaag gggaatccga tgtatacaaa
     9301 acttttacaa gcgatttatc gcgactcaga tttgtctgag aaaaccgcct ctatgattga
     9361 acaaaaagtc agtgtttatc agcagaagaa aggccaggca ggcaaactgc attgggacga
     9421 agaagatatt ttcttgatta cttatgggga tcaatttttt gaagagcagc aaccaacttt
     9481 gcgtacattt aagaaatttt atcaaaagta tttggcagat acatttagag ttgtacattt
     9541 cttgccattt ttcccatata catctgatga tggtttctcc gttgtaaatt atcaggcaat
     9601 caacgaccgt ttgggcacat gggaagatat agaagaaatg agaagcgaaa cacggttaat
     9661 gttcgacttt gtctgcaatc atatgtctgc cgaaagtact tggtttcagg aatatttaaa
     9721 cttgaatgat acgtacaaag atttttttat tgatttgcca ctggatacag atacaagcag
     9781 cgtgactaga ccaaggacca gcccgttact tacaacgttc attgacaaaa acgggaaaga
     9841 aaaacacgta tggacgactt ttagcgaaga tcaagtagac ttgaatttta aaaatccaga
     9901 agttttattg aaaatgatcg atattctttt gttttactta gaaaaaggcg cttcatttat
     9961 tcgcttggac gctgtgggat ttttatggaa agaactagga acttcttgta tacacctgcc
    10021 agaaacgcat aaaatcatcc aattattccg tgcaatcgct gatgaagcag ctccaggaac
    10081 ggtattgatc actgagacaa atgtgccaca caaagataat atcagttatt ttgggaatgg
    10141 cgagaatgaa gcacagatgg tctatcagtt ttcgctacca cctttgaccc ttcaagcgat
    10201 tcgttcgggt aatacaagta agttgaaaga atggttaaaa accatcgatt tcgcatcgaa
    10261 aaaaacaact ttttttaatt ttttagcctc ccatgacggt atcggattga atccggtacg
    10321 aggtattctg ccggaaaaaa cgatccttga attagtggaa cagctgaaga aagagggtgc
    10381 ccgagtaaac tacaagaaaa acccagatgg aacgaccagt ccatatgaga taaacgctac
    10441 gtatctagat gcgttaagtt ctccaacaga tacgacggaa tggagaagca aacgcttttt
    10501 agctgctcat tcatttttgg taacgttacc aggcgtgccg gcaatttata ttcaaagtgt
    10561 gctgggaagt cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa
    10621 tcggcaaaaa tttttgttag cagatattga gaaagagttg cagacagatc cattaagaac
    10681 agcagtatat ggtgaattga cccagatgct atctattcga aaaaaagaaa aagccttcca
    10741 tccgaatagt ttgatttact tactggactc agcagattct tttatcagtt ttattcgcga
    10801 agcgaaggaa gagaaagtct tagttatcca taatttttcc aatcaatcag caggttatct
    10861 tttaccaaaa ggagattacc tagacctgtt ttctggacaa attatccatt gcagtcaaga
    10921 acgaaagttg atattccagc cgtatgaata tatgtggctg aaaataataa aataagaggt
    10981 ggaaaaaaat gaaaaaaatt attgttacta cctgtctgct agctactgga tttcttctta
    11041 ctgcttgtgg tgaaagtcaa agctctaacg aatcacaaca gacaacgatt caattcatgc
    11101 actcttctgt cgaacaggaa cgattaagtg tgatcaatga actggttgct gattttgaaa
    11161 aagaaaatcc agacatcaaa atcaagcaag tgccggtaga agaatcagcg ttcaatacaa
    11221 aaatcgttac gttagctcgt tccggtaaat taccagaagt aatggaagtc agtcaggatt
    11281 ttgctaaagt gatggataaa gatgaactga ttgatcagac ggcagtccaa aacgttatcg
    11341 aggaagctgg agaagataat tattatgacg gagccaaaaa ccttgttcgt tcagaagatg
    11401 gaaaaagcta tattgctgca ccaattagcg gctgggttca aggtatttgg tatgacaaag
    11461 aaaaattatc agaagctggt ttttcagaac cagaaaattg ggatgatatt ttaaaaattg
    11521 ctcaacattt cacagataaa gagaataaac aatatggaat tgctatgcct actgctgaag
    11581 gaacaatgag tgagcaagcg ttctctcaat ttgctttgtc aaatggagca aatgttttgg
    11641 atgatgaagg cgaggtaacg atcgatacgg aaaagatgag agaagcattg tcgttttatc
    11701 aaaacttatc ccaatacacg atgccaggct ctaatgatgt gacagaaatc aaagatgctt
    11761 tcatgaatgg aacagcacca atggcaatct attcaactta cattttgcct tctgtttatg
    11821 aagcggggaa ctcagaaaat atcggttttg ctatcccaac acaaaaagat caagctgtct
    11881 atggcacagt atctggattg acgatttctt ctggtctaga cgagaaacaa aaagaggctg
    11941 ctgaagcatt cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt
    12001 ctcctggtgg tgctcaacca gtgaataaac aagtagtcga actggatggt tacaaagaaa
    12061 atgaagtcat caagtctttt ggcgagctgc catctgagat tgctagtgct tttgatgaag
    12121 tacaagtctt tggactagtc ggggaaaaga atttcacgaa aatgggagat atcacaagct
    12181 caggtgtgat cggaaaagca gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag
    12241 ctttagctga cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa
    12301 aaagtagata gctattggca gattatcagg gggaggtttc aatgaatcgt ccaaggaaac
    12361 attctgactt aaaattagcc atgggacttt tagcaccaag catgctttta ttagctgttt
    12421 tagtgctgat tccaatgctt tccaatattc agatcagttt tttcgatatg ccgctgaatc
    12481 ctaactcaga cgccgttttt atcggattaa gcaattatct tgaaatttta agtgatagtg
    12541 aattttatcg ttctttaggt ttaacagttg cttacacatt cttagttgta gcaggcagta
    12601 cagctttagg attagccgta gctattttgc ttaaccgcaa gttcagattt aggaaaacgg
    12661 tgcgttcact gattttatta agttatgtta cgccatctat ttctttgatt tttgcttgga
    12721 aatatatgtt caataatagt tatggaatcg tcaatgatct gttagtagat aggttgcagc
    12781 ttttcaacga agcaccgctg tggttcgacc agccagttgc tagcttcttt atggtcgttt
    12841 tgtttgctat ctggagatac tatccgtatg cgtttatttc atttttggct attttgcaaa
    12901 cagtcgacaa aacgctttat gaagcagctg aaatggacgg tgcaaatgct tggcagaagt
    12961 tcaagatcgt gacactgccg gcaatcaaac cagtactagc aactgtgatt gttttacgat
    13021 ctatctggat gttctatatg tttacagatg tctatctact gacgaataaa gtcaatatct
    13081 taggcgttta tttgtatcag acagcctttg cgttcaatga tcttggaaaa gcagcagcta
    13141 tatcgattct attgttcatc attattgcag cggtcgtctt gctctcacga aagaaggtgg
    13201 acttgaaatg aaaaataaaa agagcaggaa aatcttattt tacgcgacga ttttgttgct
    13261 attagctgtc aatttgtttc catttttgat catgctgatg acatcattca aggattcaca
    13321 agaagcaatt gcaacgacac aaactatttt tccgaaagaa tggacttttg aacattatca
    13381 ggatattttc aatgctgaga tttttccatt catcatgtat ttcaaaaata gtttagtgat
    13441 ctccttgata gcctccgtaa ctgcagtagt gattgggatc agcggggcat acgctttgtc
    13501 aaaactacgc ttcaaagctc gctcgaaaat caatgctagc ttttacaccg tttatatgtt
    13561 ttccggtatt ttgatcgttg ttccattatt taaaatcatt tccggtctag gtttgtatga
    13621 taccaataca gcactgatta tcacgatggt cgttcaaaca cttcctactg cgatattcat
    13681 gctaaaaagt tattttgaca cgattccgac tgatattgaa gaagcagcaa tgatcgatgg
    13741 tcttagccgg atccagatca ttattcggat cattatacca ttggctattt ctggaatcat
    13801 ttctgtcttc gtttattgct ttatggtttc gtggaatgat tatttatttg cttcgatttt
    13861 cctgtcgacc tccgaaaaat ttactttacc aatcggtttg aacacattat tcagtacacc
    13921 agactacatt tggggacgaa tgatggccgc ttcattggtg accgctttac cagtcatcat
    13981 tatgtatgcg ttatctgaac gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg
    14041 ataaaaagaa aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa
    14101 tagccggatt caaagattac gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag
    14161 taattttcgc tactccaaaa ccaggaacag aaattgtcga ctttagagga ttaagtccat
    14221 ttatcgatga agaattttca gaagaatggc gcctattctt gccaagagaa gaaggaagcc
    14281 aacgcggtgt ggtatttggc gaatttcaat taggaaatat gattgtcggt acgatcgaag
    14341 aaacgggcag tttggtgaca gagtatcaaa aaggagagtt agtctgcact tatggaccca
    14401 ttcgtgaaac gctgattaca aacggtgttg ataattataa actacgtaaa cttgatcggt
    14461 tggatcaatg gaaaaatgca gtatgttacg atcctgctca attcgcttta ggtggtatcc
    14521 gggaagcaca tgttcgagca ggtgatgctg tagcaatctt cggtttaggc gctatcgggc
    14581 aaattgcgat tcaattagct aaaaaagcag gtgctactac catcgttggg atcgatccca
    14641 tagcgcagcg tagagaatta gcagaaaaac acggtgccga ttacacgcta gatcctatga
    14701 aagcagatgt cggttttcgt ctaaaggaat tgacagataa acgtggagta gattcagtta
    14761 ttgaaacgag tggcagtgct gccgcattac aagcttcttt aatagggatc ggctatggcg
    14821 ggatcatttc ttatgttgcc tttgctaaac cttttccagc tgggtttaat cttggtcgcg
    14881 aagcacattt caataatgca aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag
    14941 attacccaag atggaaccgt aaacgaatcg aggacacatg ttgggaatta ttgatgaacg
    15001 gttacttgga ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa agcgcagaag
    15061 cttacatgcg ttatgtagac cagcacccag aagaaagtat caaaatgggc gttcaagtcc
    15121 gaggaggaga acaatgaaat tcggtacgca agatcaagca tttttcccaa gagacatttt
    15181 agaaaaattt agatttatca aagactgtgg atttgaagaa tacgaaatcg acggtaagtt
    15241 gttaatagaa aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt
    15301 gacagcttgt aacggctacg atgggtggat cggtgacttt atcgaagaac ggcgtcttaa
    15361 tggtgtacgt caaattgctg agattttgcg tgcactaagc gaggttggag gcaaaggaat
    15421 cgttatccca gcagcttggg gaatgttcac ttatcgatta ccgccgatgg tttcaccccg
    15481 aagcaaagaa ggagactata aagcagtcag cgaatcactt cgttatttag ataaagtagc
    15541 cgaagaaaca ggtacggtta tttatttgga gcctttgaac cgataccaag accatatgat
    15601 caatttgtta gcggatgcgc gtcattacat tgactctaat caattgaaac atgtgcagat
    15661 catcgctgat ttttatcata tgaatattga agaagatgat atttcaaaag cattgactac
    15721 gcaaaaagag tatgtgggtc atatccattt agcagataat catcgctacc aaccaggatc
    15781 tggcgcaatt gattttactc gccattttca aacgctactt caaaatgagt atgaaggtta
    15841 tatggtatat gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt
    15901 gcaatacttg aaaaactgtc tggaaacagc gcgtcattta aatgatttag agaaagaata
    15961 gagaaagaat aacaataagt aaataagcaa aggagagaga aataaatgaa gaaagtagca
    16021 atcataggcg ctggacaagt agcagaaaaa gtacatgctg cttattatca aaaaagatca
    16081 gatgtcttag aattggccgc tgtggttgat ccagtcagcc agcgagcaga agacttttgc
    16141 cgacgtacag gattttctca tgcgtataca tcagttacac aaatgctgaa tgagatttct
    16201 cccgatatcg tgagtgtctg tacacccaat cgctttcact tcgaacatgt attggctgcc
    16261 ctcgaagcta atgcttcagt gatgtgcgag aaaccaccag ctatgacagc tgatcaagca
    16321 agaaagatgg cagagacagc cgataaaaac gggcagattt tggcttatgg attccagcat
    16381 cgtttttctg acgaagcaac aatgatcaag aatcatcttg atgttctcgg ggatatttat
    16441 tacgtagaag cgaatgcgct tcgtcggtct ggtgttccag gctggggaac gtttatcgac
    16501 aaagaacttc aaggaggcgg gcctttgatc gattatggca ttcacatgct tgatacagcg
    16561 atgtatatta ctggatttcc taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt
    16621 ggtacgaaaa aaaattcagg gaaattcggg aaatgggatc caagcaaatt cacagtagaa
    16681 gatgcaattt ttggcacact ggaattagaa aacggcaaga tcatccaact gaatacttcg
    16741 tttgctttga atatcagaga agacaaactg aatgtggctt tttgtggcga tcaggcaggt
    16801 gctaatctgt atccaggcga actattcgaa gatgtagatg gaaagctggt ttccttacaa
    16861 aagaccacgc cagttaatga cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg
    16921 ctcggtgacc agactcagat gattgcagat ggctggcaag gatatgtcat ccagaatctg
    16981 gtggaaaatt tatatcgttc ggcagaaaca ggagaagtta ttaccttatg aaagtagaag
    17041 aaaaacagtt tgatttagag tatatcaata agtatgcgag tcagatgacc attggaaatg
    17101 gattttttgg tatcagagga agccaggagg aagactatcg gaatcaagta aggggaatgt
    17161 ttgcagctgg tgtatataac cgacctttag gagcagaatc agccgagtta gtgaatctcc
    17221 cagatgtcat cagatgggaa ataaaaatcg acggtgaaca tttttctctt gagacgatga
    17281 aagttcttgc atacgagcgg gcactagatt tagaaatggg agagcttaaa cgctatgcgc
    17341 tcattgaaac tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa
    17401 ataatctcca tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag
    17461 tagatgtgaa aacgggtatc gatggacagc aaaccaattt tggtacacag cagttggttg
    17521 aaaaagaact gcgagtattt ggtgacgatt tgatcagtgc atcctaccag acaacagaaa
    17581 gtaaaattga tattgggatt gcagctaaat tcaataaagc aggtgtattc caagcaaaaa
    17641 atcgccggat caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg
    17701 agaagatcat cgtgctctct acctctcttc agcaaacaga cccgtttaag aaaagtattg
    17761 cccaagccca gaaaagcgtt agttatacag aaattttaga gcagtcgatg tcttattgga
    17821 cagatttttg gcatcagcat cggataacga tccaaggcga ttcttctttc gatcagttag
    17881 cgatggactt cgctgcttac catttacaga ttatgactcc ccgcaatgat ccaagtgtta
    17941 gtgtaggggc gaagggacta actggcgaag gttataaagg gcatgttttc tgggatacgg
    18001 aaatttttat cctgcctttc tttttatatc agtttcctga agtagcaaag aatttgctgc
    18061 tgtaccgtta tgatcgatta gaaggcgcac ggcaaaaagc aagagattat cactatcaag
    18121 gtgcactttt cccatgggaa agcgcacaca ctggtcagga agagacaccc aaatttgctg
    18181 cattgaatat caaaaccgga acaagacaag aagttgcttc agctgccgca gaacatcata
    18241 tcagtgcaga ccttgcttat gccgttcaag agtatgtcca agcaacagag gatcaatcgt
    18301 ttttagaaac aaagggaaaa acgctcattc atgaaacagc agctttttgg ctgagcagag
    18361 ctgtaaaagt cgatgatcat ttagagatac acagagtcat tggtccagat gaatacacag
    18421 aatatatcga caataatgca tatacgaact atctggcgca ttataatgtg gccttagctg
    18481 ttaaatatca agctggcgat ccgctttttt tgaaagaatg tcaaaatttt ttagataagt
    18541 tatatttgcc agtgcctaac aaagaacagc tgattccgca agatgacacg tatctcagta
    18601 agccagttat tgatttgacc acttacaaag aacgtcctgg cagccagtct attttacttg
    18661 attatgcccg tagcgaagta aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc
    18721 tgttatactt attacctgag tatttttctc aaccaatcgt ttctaagaat ttgcattact
    18781 atgaaaacaa aacaatccac gactcatcgt tgagtaaagc gattcacgcc atagaggctt
    18841 gccgtatcca agaaatggaa tgggcgtatc atttattcca agaagcgtgc ctgattgatt
    18901 taggaccaga tccgcattca tcagatgacg gaattcatgc tgcttcatta ggagcactat
    18961 ggctagcagt tatatttggt ttttcaggta ttacagtaac gaatggaact ctttctattt
    19021 ctccaagact tccttcagca tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca
    19081 ggttgctatt tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat
    19141 tacctatttt tgtcaatgga gaccaatacc agctggaaaa agaactgatc ttggaacgga
    19201 ggggaaaata atgcgtattc tgattgcgcc agattctttc aaagaaagtc tgacagcctt
    19261 agaggcagca caagccattc aatcaggttt tcaagaagtt tttgttcagg ctgaatatga
    19321 tctgctacct ataggcgatg gaggagaagg aacggtttca tcattagcag acagtttaag
    19381 gttgaagcgg caaacagcag tcgttactaa tgcatttggc aaaaacaaag aaatccagta
    19441 cgcttcagac ggtcaaactg cgatttttga aatggcagaa atcgtcggat tgaaagatgt
    19501 cccactaaac caacaacagc cacttaggtt atccacgcga ggagtaggag aactgatacg
    19561 acaattgttg caacaaggtg ttcgacacat catcatcggc gttggcggaa gtagtaccaa
    19621 cgatggcggc atcggcatgg cagaaggtct aggttatgct ttttacgatc aagataatca
    19681 aaaagtagaa gcactgggag aaaatctagg taaaattgct acagttgata aaacgaacgt
    19741 gcctattgag ctgattaaag ccaaaatcac tgtcattact gatgttacta atcttttatg
    19801 cggaaaagaa ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt
    19861 agcagtggct gaccaagcca tgcatgattt ttatatgaga tttgcccctc aagtaatcga
    19921 ggcatcgggc agtagtgctg gtggtggaat gggtgcagga ctgttagcat ttgcttcagg
    19981 gaaaatagtc tctggcatcg actacgtatt ggatttactg gagttcgata aacgggtaca
    20041 gcaagcagac ctcgtaatcg taggcgaagg acgtctagat ggacaaagtc taaaaggaaa
    20101 agcgccagtt gggattgcta gaagagtacc agagggcgtt cctgtgatag ctatttgcgg
    20161 aagtgttggt aaaggaagcg aaaaagcagc agatgttggc atttcggccg tttttccgat
    20221 ccttcctgca gctgtgcctt tacaagaagc tttacagcaa gccactgata atcttcatcg
    20281 cacagctaaa aacatcgcat ctttacttca agtcgttcat tttcaaggag gtaatcgatg
    20341 aaaatcaaag cagcactatt cgatttagat ggcgttcttg tagacacagc tcgttatcat
    20401 tacgaagcgt ggctggtttt agctaaccag ctgtccattc cattcactga gaaagaaaac
    20461 gagcaactga aaggaatatc cagaaccgag tcactagagc gattgctgtc atttggaaaa
    20521 atggaacaga aattctcaga aaaagagaaa tcagcctttg ctgagcaaaa aaacaatcta
    20581 tatcttcaag cgattcaaaa aatggatgaa acatccgttt tgcctggtgc aatagctgtt
    20641 cttgagtact tgaaaaaaac aaacatcaag atcggattgg gatcagcaag caaaaatgca
    20701 cgcctcattt tagaaaaaac gaatttgaca agttattttg atgtccttat tgacggcacg
    20761 caagtatcaa aagcaaaacc tgatccagaa gtttttctca aaggagcaca gcaattgaat
    20821 gtaccgccaa atgcatgctt ggtcattgaa gattcagaag ctggttgtca agcagctttg
    20881 gctggtaaca tgcatgtatt gggaataggt gaaaacataa atcttccttc tgctgaatat
    20941 gtgattcctg atttaaccgt ttttgatcaa gtacgttcgt tttggcattt atcagaagcc
    21001 gtatcacgta tataatagtt tccgtttttt ctgtcacaag caaaacgtta tcctcctttt
    21061 ggaaatcaag gtatgatgag aaaaaagggg gattttatat gacaaaaaaa gcagttttcg
    21121 caggcggatg cttttggtgt atggtcaaac catttgaaga gcagccaggg atcatttctg
    21181 tgacatccgg ttatactggc gggcatgtac caaatccaac ttatgaacaa gtaacaacag
    21241 gcacaacagg gcatacagaa gcagtagaga tcgaatatga tccagaagtt atttcttatg
    21301 atcagttggt ggaaatctat tggcagcaaa ccgatccgac tgacgcatta ggacagttcg
    21361 ctgatcgcgg tgattcttat cgtccagtga tctactataa tgatgaagaa gaacgaaata
    21421 ttgcagagcg ttcaaaacaa gcattgcaag acagcgggcg attcgataag ccaatcgtga
    21481 caaaaatcga accaagaagc gaattttatc ctgcagaaga ttaccatcag gattattaca
    21541 agaaaaacaa gctccattac aatctttatc gagaaggttc tggacgtgca ggtttccttc
    21601 gtcgacattg gggcgtcaag aaataagaag ccgaaaaagc tacttatgga acaccgtata
    21661 tcagaggttg taaaaccagc attttgagct aatactgagg gtcagctgat tgaacaccat
    21721 ttagtggaga ggttgtgaac ctgtcgccta gctccaagtc acaaggattt ggtaagagaa
    21781 cactatatat cagaggttgt gacaagtcgt ttgtcacaac cttttttcaa tacctaagat
    21841 gaaggacaat cagcaacgca ctgctcaaaa aaggaatgaa aggcagcttt tccaactttt
    21901 ttttatgaca gataaagtaa acagaaaaaa cggatattcc aaacagacta gcaagaaaca
    21961 agatctgaag caactcttct agagacaagc caccactcca agaaagcatc aacagagtat
    22021 cgccagctcc gagataggat tgaccatagc gaaggcacac accgcctacg ataaacgtta
    22081 acagcacact ttgccattgg aagacgccgg cctgcaacca aaaaatccat aaaattgccc
    22141 aagaacagaa tagcgtgtga gaatggacca tgaagtaaaa taaatccatc aacgaaaata
    22201 gcaaagccga aagcatccat atgaacgtcg aaaaatttct tgcggaagaa aaagtaaaga
    22261 accaggcaaa aagaccacca cataaggctt ctgccaacaa atagtagaag ggcaaccgac
    22321 acctgcaata gcggcagcga aatcgttgga cgatgatcga gagaatcgga atcaattcat
    22381 accaagaaag cgaacgacag catttgacac aatgagatct aggataaata atagagtgac
    22441 ctaacggaat ccgctgggcg gttaaacata aaaatgatcc gatacagcaa ccaatgataa
    22501 aataaagtaa cacgccatct cccccttttc aaaaccaaga tacgcaagct taaactaaat
    22561 ttttgtacat acataaaatt tgaggtgaaa aaatgaacgt ttcaatcgga ttagatatcg
    22621 gaaccacaca aaccaaagca gttgctttca cagatgaagc ccaagaagca gcatccgctt
    22681 attttcgcta ccctttgatc caagaaacac aagggatggc agaacaagat caggaattga
    22741 tctttcaagg tgtctgcacc gttataaaag aagtaattaa acagttgcct tcagacacaa
    22801 tcattcaatt tgtctccttc tcctctgcga tgcatggcct gatattgttg gatgaagaga
    22861 aaacaccgtt atctcgaatg atcacttggg cagataaccg agcagcggac gaagcagaaa
    22921 aactgaaata taccgtgctt ggcaaagaat tttatcggca gactgggacg ccgattcatc
    22981 caatgacacc attgaacaaa atcaagtggc tgcaaaacag ccaacctgaa ttattcaaaa
    23041 aaactgctta ttttgtcggt atcaaagact atatctttta tagattgttt ggcgaactga
    23101 tcagcgacta cggaacagct tcaggaactg gatattttga tatccatgaa ttccaatggg
    23161 cagaagaagt gcttgactac ttgaacattc gaatagagca attgccgcaa gtctttcctt
    23221 ccacctatca agtaacaggt ctaccatcaa aaacagcagc agaactaggt ttgccaaatg
    23281 atatatcatt tgttttggga ggggcagacg ggccgctttc taatctaggg ttaggtgctt
    23341 ttggtgatac gatcgccgct ttgaccgtag gaacgagcgg agcacttcgt tttattacta
    23401 ctcagcctca gttccatcca gaaatggaga cgttttgtta tgttctggat cagaaacatt
    23461 gggtagtggg aggagcgact agcaatggtg ctggcatctt tgattgggca gggcatacat
    23521 ttttacagga agtcgttcaa tctgctaaag aaaaaggaga aaatccatat gatgcactgc
    23581 tttcggctat cgcttttgtg ccagcgggtg ccaatggtct gatttttcat ccctatttac
    23641 ttggtgagcg agcaccgctt tgggacgcag atgcgtttgg cagttttatc ggtttacagc
    23701 ggcaacatac agaaaaagaa atgatgaagg cgattctaga agggatctgt ctcaaccttt
    23761 atcggatttt aaaaggagtt tgccaggaaa atcagctcac agagatccgt gctacaggcg
    23821 ggtttgctca atctccgatt tttcgtcaga tgatggctga tgtactgggt tacccgcttg
    23881 ttttccctac tgtcacagaa gcatccgcat taggagctgt gattttaggc tggagaagca
    23941 tcggaaagat ccaaagtatc agtgaagcac aggaattgat agaattgaaa gagcaagttg
    24001 gagtacaaga aactgatcat aaaaaatatc agaaaatcta tccattattt gtttcgacac
    24061 aaaagcattt agcacagact tatcagcaat ttgctcaact gcgagaagaa ttagctgatt
    24121 ctgaaaaatg attggcttta cggataaaaa agcgaactga aaatcgttct caggtaaatg
    24181 attttgttgt acttttcatt gaaagtctac tattatttta agtgtaagaa aatgttagga
    24241 ggcaagaaag atgaaattcc aacaaaccaa agaagtatta aatcaattag ttgcagatct
    24301 aagccaaatg tctgttgttg ttcaccaaac acattggtac atgagaggac caggtttcct
    24361 tacattgcac ccaatgatgg atgagttcat ggatgatttg aacgctcagt tagatgaagt
    24421 atccgaacgc ttgatcacat tagacggttc accttattct acattgcgtg aatttgcaga
    24481 taacacaaaa atccctgatg aaattggccg ctgggatcgt actattccag aacgtttgga
    24541 aacacttgtt gcaggctatc gttatctagc agatctttac caaaaaggaa tcgaagtttc
    24601 tggagaagaa ggcgacgatc caacacaaga tatctttatc gaattcaaaa cagccacaga
    24661 aaaacgtatc tggatgatcc aagctcattt gggcaaagcg ccagagatcg acgcgtaaaa
    24721 actgttgttg aacttgaccg aaaaagaggt tgtgaaatca gcgttttgac ctgagtatta
    24781 gagaaacaaa cgtcaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    24841 tcagctgatt gaacaccgtt taccaagagg ttgtaagcct gccgttcagc tccgagtcac
    24901 aagaaacaat tttaaaaaac agcttcttat gttttatgga tattgtgact tgtgaccgag
    24961 gagttggcag gagaacaccg tttattcaaa ataaggggtt gtgacaagac ttttgtcaca
    25021 gcctcttttt gtttttcatt ttgacaggaa acgacccttt tatataaaag aaagaaaaga
    25081 agtaaagttt catataaatt tttcaatcaa gcagaaaaac gttgacattg actagattag
    25141 cgggtaatat gttaaatggt attgtttgcc ccacaaagag ccccggaaac tcgaagtgta
    25201 tgtcaaacat cagaacttaa attggaggaa aataacgtgc gtacaacata tatggccaaa
    25261 ccaggcgaag tagaacgtaa atggtatgta gtagacgcaa ctgatgttcc attgggacgt
    25321 ctttcaacag ttgttgcatc tgtactacgt ggtaaaaata aaccaacttt cacacctcat
    25381 ttggatactg gcgatttcgt catcgtcatc aatgcagaca aagtgaaatt aacaggtaaa
    25441 aaagcgactg acaaaattta ttaccgtcac agcaactacc caggaggatt gaaatcaatc
    25501 actgctggcg aattgcgtgc taaaaattct cgtcgtttga tcgaaacttc tgtaaaaggt
    25561 atgcttccta aaaacacttt aggccgcaaa caatttacaa aattgaacgt atacggtgga
    25621 gcagaacatc cacatgcagc acaacaacca gaagttttag acatcacaaa cttaatctaa
    25681 taggagggaa tttcattggc acaagttcaa tatatcggca caggccgtcg taaaaacgca
    25741 gttgctcgcg tacgtctagt accaggaact ggcaaaatta ctgtaaataa aaaagatgtt
    25801 gaagaataca ttccacatgc tgacttgcgt gaagtaatca atcaaccttt cgctgttact
    25861 gaaacaaaag gcgcatatga cgtatttgtt aacgtaaacg gcggaggata cgctggacaa
    25921 tcaggagcta tccgtcatgg tatcgctcgt gcattgctag aagttgatcc tgacttccgt
    25981 ttagctctta aacgtgctgg cttacttact cgtgacgcac gtatggtcga acgtaaaaaa
    26041 ccaggtctta agaaagcacg taaagcttca caattctcaa aacgttaata tcctttatat
    26101 atcaacgttt ctggcacttt tcaattattt ggaaagtgct tcttttgttt ttaagaacac
    26161 atttaagaac acatatcaat cacacttatt caatcctctc tgaaaagctt caaaaacagc
    26221 cttatcaact atatctggtg tgttcacata tatctttgtt gtagaaatat ctgagtgtgt
    26281 taaagcattt gaaatttgat tcatatctgc tccaccttgt ctagctatag ttgcataagt
    26341 atgacggagt ttgtgtggag ataaatgaac taagtcagga tgccttcttt taattgaatt
    26401 gattcgatag tttaggtaat cggcatgaac aggacaattt acttctccag atggtttagt
    26461 ataagtaaag agatactgat ctaacgtttg cttaattttt aatttcaaca attgatcagc
    26521 ttgtaccgat ttccattcac ttagcaatgt cattacaact tctggtactt gaatgacggt
    26581 atcttttcga ccttttgtaa acttttttct ttgatattta tctaaagtat gtttaagacg
    26641 gaccgtttgt ttttcaaaat caatatattt ccattgcaaa gcgtaagtct cactttttct
    26701 atcaccaaga tacagggtaa tcataaaaag tgtgtaatca tgaaaagtaa gtaaatgatt
    26761 attaaaatca tcatttactg cttctatcca attagtaagt tcttttgatg atagtgcttg
    26821 cttaccttct tgtattcttt tttcttctaa agcatttttt ttaggtgcag tgatactttg
    26881 aataatttta gtagttcgat taaattcgat ataatttagt atttctgcaa tatcgaacat
    26941 ggaacgaaca tatcctttta tgatttttat atttgcatat tctttcgatt ttttggttaa
    27001 ttcatctgat attatctcag tattgatatt tagataattc attgcgtatt caccaaacat
    27061 aggtaaaata tgcaaccgaa atatatcttt tgtattacta atggttatat cactaggagg
    27121 cctgtttgat ctaattgttt gacctaattc atatctagga agccatttag attcgtaaaa
    27181 ttttttaaac gttatttttc cttttaattc taatgagttt gcattctcct ctctcaaaac
    27241 tttttctatt ttcttcttca tatcattttc ggctttaatc gccaattgcc tatttggaaa
    27301 tgttttttga tacttttctg atgcaattcc tgttatttct ttaaaccttt ttggatatct
    27361 cagattagtt ttatatttcc catttgaaag ctttgtaggc aaaatcctca ctccttaaaa
    27421 aaagtaaggc agtatatttc actaatttta gtatatgcgg cgagaagaaa atttcaagtt
    27481 attttaaatg tattcataaa gtctttaata tcttctacgt caaagcgaac tacaccacca
    27541 actcttatta ctgggaaatt atatatacga atccacttat ctagagtatt attatcgatt
    27601 cctagaaatg cacaggtttg ttttttattc aggtagactc tggattctat aaaatcatct
    27661 cctaaaatag tagttatgaa atttttgaat tgctttgggt aagcacatag gaatttcacc
    27721 tctccccagt ttacggcgag ccttacggaa gtatcattat ttgctgctat tgtcatggca
    27781 ttttgttttc ccagaacaaa aagaaatctc taagtatttg tcgctaatca ggtcatggcg
    27841 tacttgagtt tctagctcga atagca
//
LOCUS       DAZAOG010000017        27785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000017 DAZAOG010000000
VERSION     DAZAOG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.017"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..1191)
                     /locus_tag="ACHDLD_000701"
     CDS             complement(247..1191)
                     /locus_tag="ACHDLD_000701"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN3362456.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1487..2431)
                     /locus_tag="ACHDLD_000702"
     CDS             complement(1487..2431)
                     /locus_tag="ACHDLD_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3362457.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2651..3865
                     /locus_tag="ACHDLD_000703"
     CDS             2651..3865
                     /locus_tag="ACHDLD_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3362458.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3887..5902
                     /locus_tag="ACHDLD_000704"
     CDS             3887..5902
                     /locus_tag="ACHDLD_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN3362459.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5985..7358)
                     /locus_tag="ACHDLD_000705"
     CDS             complement(5985..7358)
                     /locus_tag="ACHDLD_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN3362460.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7384..7668)
                     /locus_tag="ACHDLD_000706"
     CDS             complement(7384..7668)
                     /locus_tag="ACHDLD_000706"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3362461.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7670..9685)
                     /locus_tag="ACHDLD_000707"
     CDS             complement(7670..9685)
                     /locus_tag="ACHDLD_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3362462.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9864..10169)
                     /locus_tag="ACHDLD_000708"
     CDS             complement(9864..10169)
                     /locus_tag="ACHDLD_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN3362463.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10258..11289)
                     /locus_tag="ACHDLD_000709"
     CDS             complement(10258..11289)
                     /locus_tag="ACHDLD_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3362464.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11496..12371
                     /locus_tag="ACHDLD_000710"
     CDS             11496..12371
                     /locus_tag="ACHDLD_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3362465.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12523..13365)
                     /locus_tag="ACHDLD_000711"
     CDS             complement(12523..13365)
                     /locus_tag="ACHDLD_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3362466.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13457..15334)
                     /locus_tag="ACHDLD_000712"
     CDS             complement(13457..15334)
                     /locus_tag="ACHDLD_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3362467.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15331..16752)
                     /locus_tag="ACHDLD_000713"
     CDS             complement(15331..16752)
                     /locus_tag="ACHDLD_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3362468.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16755..20144)
                     /locus_tag="ACHDLD_000714"
     CDS             complement(16755..20144)
                     /locus_tag="ACHDLD_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3362469.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20401..21792
                     /locus_tag="ACHDLD_000715"
     CDS             20401..21792
                     /locus_tag="ACHDLD_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362470.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21865..22512)
                     /locus_tag="ACHDLD_000716"
     CDS             complement(21865..22512)
                     /locus_tag="ACHDLD_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3362471.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22520..23206)
                     /locus_tag="ACHDLD_000717"
     CDS             complement(22520..23206)
                     /locus_tag="ACHDLD_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362472.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23212..24285)
                     /locus_tag="ACHDLD_000718"
     CDS             complement(23212..24285)
                     /locus_tag="ACHDLD_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362473.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24507..25181
                     /locus_tag="ACHDLD_000719"
     CDS             24507..25181
                     /locus_tag="ACHDLD_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3362474.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25186..26526
                     /locus_tag="ACHDLD_000720"
     CDS             25186..26526
                     /locus_tag="ACHDLD_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN3362475.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26675..27661)
                     /locus_tag="ACHDLD_000721"
     CDS             complement(26675..27661)
                     /locus_tag="ACHDLD_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN3362476.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8099 a         5248 c         5045 g         9393 t
ORIGIN      
        1 tgaatcattg tatttgccgt tgcggaagga ttttttaatt aaccacagat aaacattgat
       61 aacgattcgc ttattgtaat caacagatta aatttcatat gttcatatgt acggatacat
      121 tttcggaccg attaatttta accattctgt ttatttgctt acctattaga tatcaataaa
      181 aaactgctta acagctattt cagctgttaa gcagttttag gatttttagt tcatattcat
      241 tgtttcttat aatacagtgt tcaatacttc tcttaaagca cttgttgatt tgctgaacat
      301 tgctttttct tcttcgttga ttgataattc aacaacttca cgaacaccat tttcgtcaac
      361 gattgatggc acacctgtaa agatatcttt ttcgtcgtat tcaccagtta aataagcaga
      421 tacaggcaat acagcttgtt cgttattcaa gatagctttt acgatacgtg cagtactcat
      481 accaatacca tagtaagttg cttttttacg atcaatgatc tcataagcag cattacggac
      541 tttatcagca atcacatcta attcatcttg ggcaatacga tggtcttttt ctacaatttc
      601 aaacactggt ttaccaccga cagttgtatg tgaccatgca gcaacttctg aatcaccatg
      661 ttcacctaag atatagccgt ggacgctgcg tgggtcaact tttaatttca atgcgatttc
      721 tttacggaaa cgagttgtat ccaatgttgt tccagtaccg ataacacgtg atgttggcag
      781 accagattcg ttccaagcga tatatgtcaa aatatctact gggtttgaag caacaacgat
      841 aataccgtcg aatcctgatc ccatgatttc ttttacgatt tggcgcatga ttgacgcatt
      901 tgttttaact aaatctaaac gagattgacc aggtttttgg ttgataccag ctgtgataac
      961 aacgatatca gcatctttac attcttcata gccacctgac catacagcca cattttcatc
     1021 gccccaagcc ataccatcta aaaggtccaa tgcttcccct tctgcttttt cttgattcac
     1081 atcgatcaat actaattcat tagagattcc ttggttgatc attgcataag cgatacttgt
     1141 tccaacaaaa cctgtaccga cgattactac tttacgactt gtttttttca tgaataaaca
     1201 gctccttttc ataactattt gttactttca tgaaaagtat agtcttgttt attcatttta
     1261 tcaacatgtt aattataata gttgttatta taatttttga tggaaacgaa ttcctgaata
     1321 cgcataacag ataaatgaca aatgtttaca cctatatccc ctgataatac aaccaaaagt
     1381 ttagaactga aaataaatcc aaaaaaattc gctgcaacga tataataaga cagcaatcgc
     1441 acctaaaaca attttccttc atatgcttta gtgaaagatt ttcttcttaa tgtttatttc
     1501 tccattcatt gggagtacat gttgaatact tcttgaataa tttgaataaa gaagtcgttt
     1561 gtttatatcc gatacgatta gcgatttctt ccaacgaaaa actcgtttct tgaatcagtt
     1621 ccaacgcaaa gtcgaatttt ttctttagaa ccagttcttg aaaggaagca tcaaaataca
     1681 ttttcaattt attactaaga tagaccggat tgtagccaaa atgagctgct gtttctttta
     1741 acgtacaaga agtaaaatgc ttatcgatat aatctaatac tctaagcatc tcattttctg
     1801 cgcatccatc tgctgatgaa aagctttgtt gttctaataa atgagtcaat tcgatcagta
     1861 atagtgatgt atagagattt aacgaacgga tcttatgact gtatgtatca aaataaatca
     1921 acagcatatt ttcaatcaaa tgcgtcaaac gacttgtttt tccgccgcga aaatgaatga
     1981 attgtgtatg ttcccctttt tcttttgatg catttatcaa aaaagaagaa agtagattat
     2041 ttgtgcttgc caaatgattc aatatctctg tagataaggt ttcttctttt aataagatgt
     2101 tgattaaaat atcctcttgc cctgtgtatt cgattctttg agtagcttct ttatcaatga
     2161 tcaccacgtc actttcttcc atctctatcc tttctccatt aataatttgt aaacatgtac
     2221 cgctatacat ataattgatt tcaacaaaat catgtgtatg ctcgggaact tccgcatatc
     2281 gacggtgttt actgatatga atatttccct gttgaaatag atcccctcgg tggatttttg
     2341 gcggtgtctg aagatggtaa gctaagttta aatcattgat attcattcct tgttcacgct
     2401 gcagagtttc gattgattct tcgttacgca aaaaaatgtc taattcagct agttccaatt
     2461 gactccctcc tttttgtaag cggtttcttt cgcttattag ttcacgctgt actgctatta
     2521 tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg aacttcctgt
     2581 gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg cttacaaaaa
     2641 tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt cagtctctct
     2701 tattttgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag ctacttatgc
     2761 cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctctttta tggtcactat
     2821 ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat
     2881 cggattagtg attgcatcga tttgtggtat cctccccctc ttctcacaaa gttacagtat
     2941 tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt ctttggcagt
     3001 cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg gttttcaaag
     3061 tgcatttcaa ggatttggtg cagctttaat gacatttgtt gcaggacagc tagtacagtt
     3121 cggttggcaa tatacctttt tagtctacgc catcagctta cctattcttg tcttgtttgt
     3181 tttgtttgtt cctacacctg aaacaactga acttcaatcg catggcacag ccccaacaag
     3241 acaagtaatc aaaacgaatc ctaaaatgat tagttatggt atttttctct tgctggtcat
     3301 cgtggtatat aatgctgtat cgattaaatt agccacagtc ctgacgactt attctctagg
     3361 aaacgaaact aatgcagcga atatcttaac aatcatgcaa ttggcaagta tggctgccgg
     3421 agtttgtttt ggctggcttc agacaaaagc acatcgctat atgcttacac tttctttact
     3481 actgatggga atcggctttg tattgattgc cactgtgata aacctgtatg tgattgggtt
     3541 tggtgcgatc ctaacaggaa tttctttttc gttattcatt ccttacttat tcaaccaagt
     3601 aagtatccaa gcgcccaagt acgcggagag tttcaatact tcaatcttat tggttggcgc
     3661 taatttaggt actttcattg caccatatgg tttgctcttt ctctcttttg cttctcttgg
     3721 gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat
     3781 tagccttcta gtagtacaca ggacgaatag aaagacagtg ataagagtag atgaagaaat
     3841 aaaagaacaa ttgacaacaa aataaatttg atggaggtcg tgtaaaatga caaatgaaaa
     3901 acataaagat agatggcttt ggtatcctgg agactttgag atccgccatg gactgctaca
     3961 aaatttccaa cgggaagaac gaggttttga ctggccagcg tactggtaca tggatgattg
     4021 ccatcggaac gtcaagttta agagatatta ctttcttgat caaccttcca tgtttaaagt
     4081 gacgatccaa ggagttgggt atgtagaaat taatggacaa aaacatccat gtggaaaatg
     4141 gctgacttgt ccagctggta aagcaaaaat tcgtatcttt gtaggacata catcagggct
     4201 accagcgatg tttatcgagg gagacgaagt aaaatcagat attggctgga cagcaagtaa
     4261 ttttattgaa gaatatccag ctggctggag tccgctttat gtcgatatcg caaaagatcc
     4321 aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc actgaaacgg agattggcgg
     4381 aggaatctta ttcgattttg ggcgagcagt gaatggctgt gtagaactaa tatctctgga
     4441 tgaccatatc gaatctatca ctctatgtta tggagagtcc gatgtagaag ctatggatat
     4501 cgattattgc tactataagc aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc
     4561 ttttcgctat ttgtttatac cagaggtttc tactggaatg gttcaacttc aagtttttca
     4621 tgaattttta tctctttcga aacaagctgc tttttcaagc gattcttctt tactgaatca
     4681 gatctggtca gtatcagaag aagccttcaa gttatgtagt gatttgtttt ttatcgacgg
     4741 tatcaaacga gatcgctgga tttggtctgg agatgcgtat caatcattca tgatcaatca
     4801 atatcttttt tttgacgagg aaatcaataa acgaacgata ttagcattaa gaggacaaaa
     4861 tgaaatcaag cagcatatga atactatcgt tgactattcg atcttgtggt tgattggtat
     4921 cgaaaaccac tatatgatga cgaaagacta tgaattttta cgcatcgtct accctaagat
     4981 gcaatcatta atgagatact tgatggagca aacgaatgaa ttagggttta tttatgggag
     5041 agcaaaagat tggatattta ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc
     5101 agaacaagtc ttattactga aagcctatca ttccatgatc acttgcggag atatcttagc
     5161 tgaaacatat ggagagaacg aaaattatca tactcatctc tatcaagaaa aatatcaact
     5221 attgaaacaa aacctatttg actacttttg ggatgaagaa cttggcgctt ttatcgattg
     5281 ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat attttcgctg ttttattcga
     5341 ttttgctaac aaagaccaac agcaatcgat cttaaagaat gtcttattga atgacactat
     5401 cacacagatt actactccat atttcaaatt ctatgaacaa gatgctttat gcaaactcgg
     5461 acagacgaat atcgtctacc aaacgattct tgattattgg ggagggatgt tatctcatga
     5521 tgcggtaact ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa agtacagtat
     5581 gtatggagat ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc caatctatct
     5641 tttaggacgc tattttatcg gtttgtatcc aacaaagcca ggttatgaag aatttatgat
     5701 acaacctaat ctagattcat ttgaaacact gaattgtacg ctacctatca aagatggaaa
     5761 gatttcgctt gaaaaaacaa aagataagat cgtgattact tctgatagaa caggcggaac
     5821 agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa cagccacttg tcttaacaag
     5881 ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg acatatcaga agactttaaa
     5941 tcttcttctg atattgtcca agcaattttt attttgaagt ttgcttaatt ctgttcttta
     6001 gaaacaatgt ttgttctttt tccggcaaaa agaaagctag ataatacgac aagtaaaaag
     6061 aaagcgatga ttccaactgt caaggctatt tgaccgtatt ctcctagttt cccaagtaca
     6121 agtcctggta ctagataatc agtatcggca aatgtagaag aagcgatttt aagatctcct
     6181 aatactggaa ggataaataa tggcagccaa ctaatcaata agctattgaa agcagaacct
     6241 aataccgccc ctcttctacc gccagtcgag ttgccgaaga caccagaagc agctccgcag
     6301 aagaaatgtc ctactacccc aggaatgata actgtagttt gcaagcctat catagcaaac
     6361 atggatagtg ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc attaggtgca
     6421 tatggaaaaa cgataggaac atctaatgct ggtttagaat cgggtacgag ttttttagca
     6481 atcccttgga atgctggaac gatttctgcc aacaccattc gaaccccttg aaggacgata
     6541 acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa agattacagc gttgtctcca
     6601 ttgcttagtt ctctttcaac aaatccagga ccagctaaca aagcaagtgc gacatatacg
     6661 atcgacatca agagcatgat cgatacagta gaatctcgca aaaagctgaa acttttagga
     6721 atctttatgt cctctgtaga acggtctttg tttccaaaaa actttccgat ccatccacta
     6781 gaagcataac cgatatttcc ggtatgccct aacgcaactt gattgtttcc agtgatgttc
     6841 ttcataaacg gttgacaaag agcaggcgta atagttaata gtgttccttc gaagaccccg
     6901 cctaataaga tcatccacaa atttgcagta aaccccgcgc ttactaaaat gactgctgtt
     6961 agacaagaaa cataaagcat tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat
     7021 ctagcaagta aaatattcac gatcatccct actagcatga tcaaagcagt aggtgtaccg
     7081 tattgttgca aagcaatcgc cactactgct tcattgctag gaactactcc ttgagtatgc
     7141 agagctgttt ggaacatcga agcaaaaggg gttagtgaat tactgattaa accagcaccg
     7201 ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc cttggatcac ttctgaaaat
     7261 ttcttcttct gaagaacgag accaagcagt gcaatcaaag aaacgaggat ggagggctga
     7321 ctgacgattg acacaaacaa attcaacaat ttatccattg atagttcctc ccttgaatca
     7381 tttttattct tttgacaaga tcttcgttac tttttctttt acttcttcgc tgtcaatgat
     7441 cgattgcaaa ggtacgagct tttcatcagg tatagaagtt agcgtatctt tcaaagaatg
     7501 ctcaatgaag aaataatcag ccatatcgct agatacactt ccaacatccg tatgacttac
     7561 ttcaaactcg tccacatctg ctccaagatc tgcaagtgct tgtttgatgt tcatctctac
     7621 cataaaactt gttcctaatc cactttgaca tactgcaaaa attttcatat cattcgtctc
     7681 cttttcttat gatcgctaat atttcttctt ttgttgatgc gtgtaataaa tcttgaagat
     7741 tttgtttatt agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt gcctcattat
     7801 cgatagcagc taaacagatg aacaaactaa tgggatgggc tggattatca agcagatcca
     7861 caggttgttg aagtttcagc aaagacatcc ctacttcgtt tacgccatct tctggacgag
     7921 catgaggtaa tgcgatgtga tcgccaatat ggataaacgc accatactgc tccacgcgct
     7981 cgatcatagc tttgatataa ctttcttcta ttttattttc tgataatagc ggctgtgcgg
     8041 caaatgtgat tgcttcttgc cagttttttg gttgatccga taattggatc atttcttctg
     8101 ttaataattc agatagcata ggacgttgat cctccttctt ttctaaaagt ttgttcattt
     8161 tacgatttaa tacttgatag agcttttctt cgtctacgcc ttcttttaat tcgatataag
     8221 gcagtagcgc cgacaacaat tcttgggcag aaggcattga aaatcctggt atcagccaat
     8281 ctctttgtac ttgattgatc agctgatttt tttccaagtc agtcatgatt ggatgcagaa
     8341 catagagaga ctttttctta tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt
     8401 aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt agtctctgta aaaaagatca
     8461 tcgggaataa ttgctgaagt tcacttttta agatcaatga agagctaatg ccacttggac
     8521 agacgatcac ggctcttatt tttctatttc tttcttctgc gtccgctttt ctgatctctc
     8581 cgccaaatag tatagtgaaa aaaccaatct cttcttcagg gatgctcttc cctattcgtc
     8641 tttctaacgg aagtaatgct tttcttgtca tttcgtagat ggaagcatat tgatgcttaa
     8701 tttgatcgat catcacatta ggtaaataaa aagaatagga aatacgaaaa taggcaggga
     8761 ccaagtgata aaagacattc atcaataatt cacgaaacga agcaaattct acggccatca
     8821 ggaattccat gcgatggatg acttctgaag aacattccag caaaaaatca agtgcaggct
     8881 cctggacatc tccttcaacg atcgatgcta agcaaagaga gaaaaatata atctccgaat
     8941 tttgatgttc ggaatagcca aggccagtca atacttgtcg actaatgtca gtgactgcaa
     9001 gagaagccaa cagttcagca tcttttacca gttccatgga tgtttttcca attcgaatgg
     9061 ttgtcaaact caaataataa gctaattcat ccattcgatt tggtgcgaat cgaagatctg
     9121 atagtgaaat cgttttatct agcaaatctc taactttggc gtaataatca atggaaaggg
     9181 ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc
     9241 gattttttgc ttctcttctt aatattgctt ctgaccctag cagcctaaaa ccagttttcc
     9301 tagtatacga cagctgacaa tcttccactt ctaattcatt tcttatcttt ttgatatctg
     9361 acaggactgt ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg
     9421 ttgcagtcga ataaaccatc aaataaagaa gatctcttcg ctcttgttcg gtatacacag
     9481 cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc gattgctcgg aatttcttta
     9541 tacggatacg ggtatcatcc acatattcgg caaaaggagt taattgtttg atcgcctcgc
     9601 ttgtatttac ttcgatgatc tctgctgctt tttctagact gatttctctt ggtgttttca
     9661 ggagataact aattttgtag ttcacagcgc tgttcacctc ctacttatat tctagtatgg
     9721 tagcgctttt aaatcgaggg gtgtttggta caacttacag ttggattttt tttacgactg
     9781 taccgaacga tttctatcga atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc
     9841 ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt tattttttga ggtcagttct
     9901 tcaaattttt ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc
     9961 tcattaggct ttgtttcctc ttctacagaa tttgaaccag ataaagctgt ttctttattt
    10021 tctgtttcac cagagtttgt gccagctaat gattcttctt tgtcttctgt ttcaccagaa
    10081 ttagttcctg ctaaagccac ttcttcttct gttttagggg ccattgaacc tcttaatcgt
    10141 ttgtgttttt caccgtcata atttgtcatg ttggttcctc ctagtagttc tatacttttt
    10201 catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca
    10261 tatttttcct tctgattctt gaatgctttt gattccttct tccaaaattt tcatttgtaa
    10321 gattgtttct tcatctttga tgactttttc tgcgccatta actaatgtct cgtaaatccc
    10381 ttcgtagaca cggctgtaat caccaacctc ggaaattact ttttcttcat gataactccc
    10441 ttgttcatct atataggtca atacaccata atgttcgggc aaatcgatac caaagtcttc
    10501 atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg tttgtttcac
    10561 gaacatccct ttttttccgt aaacgacgaa acttggacgt tctttgatgc ggaaataact
    10621 ggattttatc gaaattttca tatttccata gaaaaaatcc agatcgaagt aatcattcat
    10681 tcgtcctttc cctaaaagtt gacgtacatc atagctgata tcatctggtt cgccgaaata
    10741 agaaagaact tgatctactg tatgacaagc atgtccatat aaatagctgt tcgcagtaga
    10801 aaattcatgc gtattttctg gtacttccgg tctgaaataa tcataatgca tttctacttc
    10861 taaaatatcc cctaattttc cgctttcgat tactttttgg actgtcaaga agtcagaatc
    10921 gaaccgtctg ttttggtaac attggataaa tagacctttt tcttttgcta atgcaaataa
    10981 ctcttctgct tcctttgacg tttctgcaaa aggtttctct actagtacat tcttaccatg
    11041 ttcgagtgcc atttttccat attcataatg aaattgacta ggtgtagtga tgacaactaa
    11101 ttggatctca ggatcatccc aaagatcaga cagctgatca gtatattcga tgtcttcgaa
    11161 tgttttccac actggttcag atggggaaaa aatttttttg atattgattt tgttcgtaag
    11221 ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt ccattgccaa gataagcgat
    11281 tgtaagcatg agtggtgcct ccttttattg atgttttcag tatatcctag aaaagcgcag
    11341 aaagtattta taatagctat tctggtaaaa aagttcagaa aaacacttgt tatcattcgg
    11401 tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa aagatagtag cggaaccaag
    11461 tgtttatcag taagatagaa tttggaggtc agatcatgct tattgaagaa aaactaacaa
    11521 aacaagagtt gtttaccacg acagagaaac ggattgtcga ttacatccgt aggaatatcg
    11581 aagctgcagt ctacatgacg attgaagaat tagcaaaagc tacgtatact tcccattcag
    11641 ctatcattcg tttatgcaaa aaaatgggat tcagcggatt taaagaattc cgtttcgagc
    11701 tggggcggga agttcatcaa ttgattgctc agttcaatca aacgattgat cctaattttc
    11761 ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc
    11821 agtcaataaa aaaagcacaa ttgcagattg aacatgaaga tttagatcaa atcggtaaag
    11881 tattgaacaa ggcacagcga atctttcttt ttgcaaaagg tgattctcaa atcacggcaa
    11941 ggaaattcca gaataaaatg gtcaagttga ataagttttt gatcatggct gaagaataca
    12001 gtgattcttc ttggaatgca gccaatttga ctagcgagga ttgcgccatt tttattagct
    12061 acagcggtcg catccatcat tatgaacgaa taatgaccta tttgaagcat gtagaggccc
    12121 ctaccttgct gattaccgga aatcagcatt cagaaatggc caaacaggct gatatgtgtc
    12181 ttgttatatc gcaggaggaa tatgattttg ctaaagtagc gactttctcc tctcaaattg
    12241 ccttcgatta cgtattaaat acgttattct cagtgatata ttctcaaaat tttgaagaga
    12301 atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt aaagaatatc
    12361 ggaaaactta agaaaagatc ctcatttagt aataagaaag atagtgtttg ataggctctc
    12421 tttgatatca tcataaaaac aggctgaagt tttctttttc aaacctcagc ctgtttgttt
    12481 acggaacgat tcgaatagat atattataga aatgaggaat agctaacttc tgtttcttat
    12541 tttgaacgat tgacgaaaaa gatatataag aaggaggaaa atcagtatac atgcagctaa
    12601 caatccatac aggaaaaatc gtttgttctc ggatactttc tgtacttctt tttgaacttc
    12661 ttttgcatga taaggaaccc ggtgccctct gacaagtaag cgatgactgt tgaccatata
    12721 aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt tcgattctca gatcttttgt
    12781 atcagtcggt tctaccgtct tgatctgatc gacttgataa gcgagtgtcc gtccattgat
    12841 ttcgatatag aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca actttgcttg
    12901 aggcagtcct cgatggctgg aaataacggc atgtgtattt tctccccccg taggataaga
    12961 cgttccttcc aataatgagg cacctttttc tagcagcaca gtattcgttt catcaaaaat
    13021 tggcaaatgg acattgatct cagggatcgt caaaataccg atcgtatgtt tctcaaaata
    13081 ggttctatcc tttttcgcag gttctttttt cttagtaaag ggatctgcac ctggatttcc
    13141 accttctttt gctaattgct ggtttttctt tgtcatgttt tcttggattt tagccatgac
    13201 ttcttcattt tctttgaccg cttgttgctg ataatgagag atgatctgct gatccagata
    13261 attattcagc gcgtcgctaa caaatggata agctagtatc cctattcctg agagaagcaa
    13321 gaaaagcatc agcagatcta gaagtttcct tttctttggt ttcatttgtg ttctccttgt
    13381 gatgactgtt tgataataaa agaaaaagga gcatttgcgt aatccgctgc tcctttttcc
    13441 aattcttgca aaaagactaa atctgactgt gcttgcgtct agcaaagtac agtccagcaa
    13501 tcagtagaag gactactcct gcaccgatat acacatagat tcccttaccg cctgttgaag
    13561 gaagtgtacc tttgtgttta tttggtattt tttcaggaga aatcaactgt cctgctgtta
    13621 cataagattg ttcatcgata gtaaatgcta cacggtttgt taatggaaca tatttttctg
    13681 gcgctttcgt ttcttccaga tagtacgtgc catattttag acctgtcaca tcgattaaac
    13741 cgttacttgt ggttgtaaaa accgttgctg attctttcgc cgatacccag ctgacggctt
    13801 ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact atcttgatca cgaacgacga
    13861 attctgctcc agcaagtgtt tggtctgatg taacgtcacc atctacttta acaaatcgtt
    13921 tgcccccagt aacgacatcg actgacggtg gtgtttgatc atttgtatgc ccgttatcga
    13981 catttgcttg gttagaaaat cctttggttg gatctgcttt ttcgttcaaa tgcatatagt
    14041 aaacgaattt caatgtaccg ccaggagtta atgaaggaat ataattcgga tcaactgaaa
    14101 ccgtgaatcc gtctgtttgc tcagtgacag aataatttac tgggtcgatt tctttatttc
    14161 catcatataa ggcataagca tacgttcctg aagaatcatt atcaaatgtt aaagcagcgt
    14221 catgagtatc gataagtttg aatgttgtgt acttgttttg cgttccttct ttatcagcga
    14281 tacctaatgg gatattgaca gaaatctcat attgaatttt ttcaccgatt gcgacatctt
    14341 ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt atcatttttg atggtctttt
    14401 ctactggtgt ttggctattt gccaggatct caaaaggcgt gattgtttgc ttttcaatca
    14461 tttccgcatt atcaggagct tttacttctt ctaaattata ttttccaact tctaaatgat
    14521 taacgatcaa ctggcctttt tcaatagatg cctcggcaaa gtcattgttg ccgatgtcat
    14581 aagaatgacc agtaatgaaa tgtttggctt tggtttgatc agttgtccaa gtgtacaatc
    14641 catctgtgac actttggatg tattttttga cgcttggtgt tccttcttct ttagaaataa
    14701 taaattctgc tccatttagt gcttcgtttt cggcagtacc gatttttgta actttcaacg
    14761 ttccatcatt accgactgta tttttagggt agagatggat agtatctagt tcttctgtcc
    14821 cgtatttata agagccatct gcttgtttga tcatctcata tacagggaaa gctaaaacca
    14881 tgtttgcggc agctgacact ccgtcttttg gttcttcttt gatcgtgtag actgcatctt
    14941 tcccattttg ttttttaggt aaagataaag tgacatttcc atcagcatct gtcgttcctg
    15001 aagcaactgg tgtaccagga gtcaaagact ggactgcttg ttttgctgca tccacggacg
    15061 ctcctttatc tcgttgcgca taaaattctt gagtgacgtt ataaactgaa aatgaaatat
    15121 cggctaatcc ttggtattga tcgaattcgc tcatttcttt cccgctgttt tggattaaag
    15181 gatcgggtaa atcagtcatt ttctttttat gaaggatgac ttgagctgct gtctcctctg
    15241 caaacacttt ttttgcgccg aagctgtttg tgagtaatgg taaaataagg aaaaggactc
    15301 ctaacataac gtttattttt ttatgatttt tcattttttc actcctgttc tttttttgat
    15361 aaacaaccag attgcaaagc ttgcgaatac cataccaaca acgcctaaaa gtgtattttt
    15421 cgtttcgttt gtttttggaa gaaatggacg gctattttct tttttattga ctactttcaa
    15481 tatactgtcc gcctctttat cctcagaaag aaaagttgaa atggtaaacg gaatttctgt
    15541 ttcacttaaa atataaccag aaggtgcttc gatctctttt aatcgataac ttcctgtttt
    15601 caacccggaa attgaaaaag cgccattttt atcggaaatc aggtgaagcg catctgattc
    15661 gttctttgtc cacttgtatc cgttggctgt ttgctggagg taatttcctg ctttgttttt
    15721 aataaggaat actgcgccag ctaatcgttt atctggttta tttgtctcga ttttgacaaa
    15781 ttgcttactc cctgtatata cagagacttt ctttgtactg acaccaaaac caaaatccag
    15841 tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct gcatctgata
    15901 agaaatggtc agtttttttc cagcatattt ttccaacgaa gggatcgaaa ataaaacatc
    15961 aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc agcttttctc cattcgatga
    16021 aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc gcactatctg acaattcgaa
    16081 cttttgatag tcaaggatat ttacaggaat tgtcgtatgc aaggaataag tgattttttc
    16141 accaatcgta aaatcatttc taggctcttc taataccttc tcaaaaggtg gatcagggta
    16201 gtcgttttct tcatttttag gatacaaatg gatagttgaa agtttttgat tgttttgtcc
    16261 atgtacagga agaacaacta ccatattctc tgccttttct ttgacgactt ctggtacttt
    16321 ggattcaatg aaaagatacg ctttatctct tttttcttga tctttagcgg ccagagaaaa
    16381 agaagcgata ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg taaacatacc
    16441 ggaagacgca ccgttttttg cgatctctgc ttgtgcttct tctaccgttt tgcccttttc
    16501 ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg aatgtgacac catttaatcc
    16561 tcgatacgtt tgtaataaag ctttttcttg tccgtcattt ggatgatttt tcggcagttg
    16621 cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt
    16681 tgctacctga gtaaaagctg gtttaaatag taacaagaag agacacatac taagccaacc
    16741 aagttttttc atatttagct gccccctctt tcttttttca aaaaaagata ccaaatagaa
    16801 aacgcacaac caatcatccc tactagatag attcctaaac ggccaattcc tcccgtttca
    16861 ggtaatggta cttttgcctg attcgtgatg tctaaagaaa tctggttgtt tttggctcct
    16921 ggtgacagaa cagattcatg atcttgtcca tccacttgaa ttgacccatc ttcgtgtata
    16981 actatagtaa ctggctcttt tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc
    17041 gtataagttc caggttttaa attttcgaat agaaaggtat ctgtttcttg tccatctttt
    17101 ggcgattcta agtccatttc tggtccctgc agtctgaatt ttgctccttt taatgtctgt
    17161 ccgttgtctt cttttttgtg taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg
    17221 agcgtccata cattatttgt catctcggta ttttttcccg tgtaaatgat ttttccatat
    17281 ttatcgattt ggaattcata atttcccggc gaagtatcat atccggcagg accagctgtt
    17341 tcaacgactc gatatttacc aggtgtgagt gaatcaaaac tggcaagacc tttttcattc
    17401 gttgtttggc tgtcaattgg ctgataagta ccatttgctt cttttttctg tagggaaaat
    17461 gctgctcctt taagatttat ctctttgtcc gtcccttgca tcgcatactt acgtactgct
    17521 acaggaactt caacaaaagg attttcaata gtcaactgga tcgtatcatc ggaagtagtg
    17581 actgtttttc catcaatggt taccgtacca tcagaagcga tcttgatttc ccaagtagtc
    17641 tttttgctta atccatgccc ttctggagct tttgtttctg tcagcgtata ggtcatttct
    17701 ttttgcaatt tgacattttc tggaagagaa taggttccgt cgccatgatc tgttagtaaa
    17761 gtatcaatag aatctcctgt taatttgaaa acagcgccaa taagatcctt ttcacctgta
    17821 gaggaatttt tcgttatttt caagtttaac ggtgtgaatt gtttagtatt tttccatgtc
    17881 attgcatcta tttgttgaga atcgtatcct ggtacaggta attctttgat tgtttggtaa
    17941 ctgaatgctt gaccttgatt attgtattga ggtagtgata atatctcttg ataactttct
    18001 ccgctatttg cagataattt gtcaatgtct gcacgttccc acgtatttgt tgtatcttta
    18061 gctggtttaa tgattcgaat atatccgttt ttccaagctg cagcatttgt cgtatgttcc
    18121 cgttgaatct caaaagtaac ttgatctgga cgatcagcta gattgttgtc aaattcttcc
    18181 cataactttt tgatgtgaag actgactccg ggagctttag cagatggtat accgaattca
    18241 gctaattcat tggtatcaat gtttggctgg aaagttgtcc tgccgttcat ttgataccag
    18301 aaatttggat ggaagtcctc attttctgtt tggattctca cttggtaatg gatttggatt
    18361 tcttggtctt ttcctaaata gatctgattg ctcttgatgg tatttccttc tatggaaata
    18421 gatggagtga ccgttgtagg acttgtcccc acactcttga ctgaaagtgt accaggctga
    18481 taaatgaatg gttctgcgat tggatcattt atttgtccat tgcttacagt tgctgagatc
    18541 tgtacagctt ttttggctag gtattcagag atatctgttg catgatcagc tgattcatag
    18601 taaagatcgc ctttttcatc tgatgaaacc atttgtcgca tacgagactc tacttctgct
    18661 tttgagagac cagctgccgg atcgctttga agttggatgc caagaccatg tatttcgatt
    18721 cctcgttctt tgagtgccat cgcttctccg atggttgcga taaacgtact gtcgattctt
    18781 ctggatagat tgttttggtc aggtgcatca tagattcttg agattagaga agtatttccc
    18841 ggccgatctt gcgtattaga aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt
    18901 acccgctgta ctttatagga aaatgttggt acaccatccg tcagcaaaac gatcactttt
    18961 ttatgtccat ttggaacgga tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa
    19021 gtaccaccat ttgtccgtga aggtgtaatg gattttactt gatttttcac tgaatcaaat
    19081 gaacccatct gtactgcacc gttactgtag ctatatcctt cgcttgagta gccgacatat
    19141 cccatattga ttttgtctgt gataccgcta tctgctaaag tatcgacaaa acgatcgaca
    19201 ccaatcttta cttcaccgat ccgattattg tcgttcatac ttcctgacca gtctacgacc
    19261 aatacgagat caagaggcgt gatatctttt tgtacatttc cacgagcatt caaataaaca
    19321 tcaaacaatc cgggtgtact tgtttccttt gcatactttc gtatcgcata gtctgcttga
    19381 ttaccggttc ctccatattc gatatacgaa tgggtccgat cattaggatc tccattccaa
    19441 gaatttatac catcccattg atttgttccg tttttattgc cttgatgatc aagaacattt
    19501 gtatttcctg taggttgcca actggcttta ggataagtgc cctgatcatc tgttgtatat
    19561 tctgtaggaa tcgtttgatt agatggcaca ctcacagctg gcaaggataa tgtaggcaat
    19621 gtaaatgggc tgacagaatt ttcgttttct tttgatgatt cttcttctat ttcgtctggc
    19681 tttggtactg acacatctgt atctttctta ttttctgtct catcaataat gtcatattct
    19741 ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa
    19801 ctcagatcga tagtttgttt agtgtttgat gagatttcca acgtaatttt cccgctattt
    19861 acctgataag catagccatc tccttctttt ctttccacat cacttaagta attttcgact
    19921 agaccaattt ctttttctat cttttcagca ctggtgttat tcgcatttag cttgatgttc
    19981 agtgcttttt ttccagactg atccgaaatg acttcagctg aaattgtttc taaaactgtt
    20041 ggatcatctg tagtgtctgc tattgcagat aatggcacaa agtttgataa actcaatatt
    20101 atcagcatga aaataacttt cagtttcttc cctgtggtcg tcatttttcc cctccttttt
    20161 ctttttttct gttttgacgt gaatagtgaa aattattaaa ataaacaggc gttacataat
    20221 tttatttatt ttaatatgat aattattata cgaagggcgc taaagggaat cacaccaatt
    20281 ttcataccaa ttggtttcat ttatgaagtt gattccttcg attaatgagc gtttcattga
    20341 aagaaaaggg ttgagggaga aaaacatatg atcaggttgt tggaaggaaa ctatagaaag
    20401 atggaaaaaa tcattcgaat tctgtccatt tctgacaagt ggatgactaa aaaagaactt
    20461 gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga gatcaaaatc
    20521 cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg tcttgaacat
    20581 ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc tacaaacagt
    20641 cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa
    20701 atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca aattttagaa
    20761 agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct taaaagtgag
    20821 ctccagttgc gtatctttgt tacctttttc tttttaagtt actacggaag acgcacgttg
    20881 ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg
    20941 aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg
    21001 catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta
    21061 cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg
    21121 aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt
    21181 tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg
    21241 ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa
    21301 atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact
    21361 tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa
    21421 cttcaatttt attccactct actgaatcag aatctgattt attatttatc tagctatatc
    21481 ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg
    21541 attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt
    21601 cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa
    21661 tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt
    21721 cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta
    21781 ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg
    21841 acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac gagatttata
    21901 gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt
    21961 gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat
    22021 ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga
    22081 tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg
    22141 ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa
    22201 aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga
    22261 actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc
    22321 accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg
    22381 tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt
    22441 gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc
    22501 cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt
    22561 ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag
    22621 tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat
    22681 ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag
    22741 cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt
    22801 taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt
    22861 ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat
    22921 tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg
    22981 gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc
    23041 ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga
    23101 attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag
    23161 aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc
    23221 tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat
    23281 tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg
    23341 catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc
    23401 taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat
    23461 cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa
    23521 aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat
    23581 attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag
    23641 acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat
    23701 cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc
    23761 tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat
    23821 cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc
    23881 agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc ctttcgtgag
    23941 ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt
    24001 tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt
    24061 cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac
    24121 aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc
    24181 tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc
    24241 atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct
    24301 atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat
    24361 ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac
    24421 cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa
    24481 agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag
    24541 tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg
    24601 atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt
    24661 tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac
    24721 agactccagt attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg
    24781 aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg
    24841 tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg
    24901 atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac
    24961 aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa
    25021 aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg
    25081 aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca
    25141 aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt
    25201 acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt
    25261 tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat
    25321 ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat
    25381 cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa
    25441 cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa
    25501 aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta
    25561 aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc
    25621 tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt
    25681 gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc
    25741 acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac
    25801 ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt
    25861 gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat
    25921 ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg
    25981 tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat
    26041 tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt
    26101 gttaaaccat ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat
    26161 gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg
    26221 gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac
    26281 cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa
    26341 gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga
    26401 tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct
    26461 gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa
    26521 aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt
    26581 tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttctttaagc
    26641 tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg cggtcttcaa
    26701 tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct tctgtcactt
    26761 tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata actgtatgtt
    26821 tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct tccaattttt
    26881 cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg atttcttcac
    26941 gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg atgtcgactt
    27001 ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt tgaattgtat
    27061 catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc attgtatcag
    27121 tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga tccgttgtat
    27181 tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca gtttctaatc
    27241 cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta tcttctgttg
    27301 tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa atatctcctc
    27361 tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag gtatcaaaag
    27421 aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc atccgatttt
    27481 catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat gctgcttcat
    27541 cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac cgttcaatta
    27601 ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt ttctcttcca
    27661 tgtaaattcc tcctagcaaa tagatttatg attggtatat tgttacttat aaatttattc
    27721 tataggagac gattgttttc tacaaatatt tagattggaa tttgattaaa acaatttaat
    27781 tgaaa
//
LOCUS       DAZAOG010000018        25629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000018 DAZAOG010000000
VERSION     DAZAOG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.018"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..503
                     /locus_tag="ACHDLD_000722"
     CDS             21..503
                     /locus_tag="ACHDLD_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN3362477.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            540..1550
                     /locus_tag="ACHDLD_000723"
     CDS             540..1550
                     /locus_tag="ACHDLD_000723"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN3362478.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1665..2378)
                     /locus_tag="ACHDLD_000724"
     CDS             complement(1665..2378)
                     /locus_tag="ACHDLD_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362479.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2551..3158)
                     /locus_tag="ACHDLD_000725"
                     /pseudo
     CDS             complement(2551..3158)
                     /locus_tag="ACHDLD_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            3642..3869
                     /locus_tag="ACHDLD_000726"
     CDS             3642..3869
                     /locus_tag="ACHDLD_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN3362480.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3885..5051
                     /gene="hemW"
                     /locus_tag="ACHDLD_000727"
     CDS             3885..5051
                     /gene="hemW"
                     /locus_tag="ACHDLD_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN3362481.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5285..6328
                     /gene="hrcA"
                     /locus_tag="ACHDLD_000728"
     CDS             5285..6328
                     /gene="hrcA"
                     /locus_tag="ACHDLD_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN3362482.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6422..6985
                     /gene="grpE"
                     /locus_tag="ACHDLD_000729"
     CDS             6422..6985
                     /gene="grpE"
                     /locus_tag="ACHDLD_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN3362483.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7036..8865
                     /gene="dnaK"
                     /locus_tag="ACHDLD_000730"
     CDS             7036..8865
                     /gene="dnaK"
                     /locus_tag="ACHDLD_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN3362484.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9016..10182
                     /gene="dnaJ"
                     /locus_tag="ACHDLD_000731"
     CDS             9016..10182
                     /gene="dnaJ"
                     /locus_tag="ACHDLD_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN3362485.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10264..10893)
                     /locus_tag="ACHDLD_000732"
     CDS             complement(10264..10893)
                     /locus_tag="ACHDLD_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362486.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            10905..11078
                     /locus_tag="ACHDLD_000733"
     CDS             10905..11078
                     /locus_tag="ACHDLD_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362487.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11123..11860)
                     /locus_tag="ACHDLD_000734"
     CDS             complement(11123..11860)
                     /locus_tag="ACHDLD_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3362488.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11862..13364)
                     /locus_tag="ACHDLD_000735"
     CDS             complement(11862..13364)
                     /locus_tag="ACHDLD_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN3362489.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13623..15617
                     /gene="uvrB"
                     /locus_tag="ACHDLD_000736"
     CDS             13623..15617
                     /gene="uvrB"
                     /locus_tag="ACHDLD_000736"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN3362490.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15627..18443
                     /gene="uvrA"
                     /locus_tag="ACHDLD_000737"
     CDS             15627..18443
                     /gene="uvrA"
                     /locus_tag="ACHDLD_000737"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN3362491.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18706..19971
                     /locus_tag="ACHDLD_000738"
     CDS             18706..19971
                     /locus_tag="ACHDLD_000738"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN3362492.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            19975..20706
                     /locus_tag="ACHDLD_000739"
     CDS             19975..20706
                     /locus_tag="ACHDLD_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN3362493.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            20841..21725
                     /gene="rapZ"
                     /locus_tag="ACHDLD_000740"
     CDS             20841..21725
                     /gene="rapZ"
                     /locus_tag="ACHDLD_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN3362494.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21722..22720
                     /gene="yvcK"
                     /locus_tag="ACHDLD_000741"
     CDS             21722..22720
                     /gene="yvcK"
                     /locus_tag="ACHDLD_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN3362495.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22754..23689
                     /gene="whiA"
                     /locus_tag="ACHDLD_000742"
     CDS             22754..23689
                     /gene="whiA"
                     /locus_tag="ACHDLD_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN3362496.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24232..24882
                     /locus_tag="ACHDLD_000743"
     CDS             24232..24882
                     /locus_tag="ACHDLD_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362497.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25069..>25629
                     /locus_tag="ACHDLD_000744"
     CDS             25069..>25629
                     /locus_tag="ACHDLD_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFN3362498.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8462 a         4339 c         5480 g         7348 t
ORIGIN      
        1 aatagaaaag agacataaaa atgattgact tggcaaagaa attcttacac gaaaatatat
       61 attttgagtt gtatcatcat cgagccattt atacaaatga agatgctttg gtaataaaga
      121 aagaacaagg atttacaggt acagagacca aaagcttatt cttaaaagat aaaaaagata
      181 ataactacgt atatctgact tttactacga aacaaacgga tttcaaagca ttagggaaga
      241 tcgtcggtaa aaggctttct attgtttcat cagaacgcat ggaagaacaa acgggacaaa
      301 agcagggcgc tgtttctccg tttggttatg aaaaacagat tccgattatt attgatgaag
      361 agctcttatc aaatgaaaaa cttgtttttg caccaggtag accagatcaa acgatggtca
      421 tccaaacggt tgacttaaac aaaatcatcc agctacttga gatgccgata tttttgatgc
      481 cggcaaacga aaaaaacaat taaccaagta aaatagtgca tacttggagg tgaagcaaca
      541 tgataagtta tccattatta gaaagaaatg caacgattgg ggttactgca ccatcctcag
      601 gcgtatcaga ggaacaccat tcgttactcg aatcagcttt aaagagaatg gaagaaaaat
      661 ctttccaagt agttactgga aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag
      721 caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct
      781 ttccgacttg gggaggagaa ttattgattg agattctcga ttttgtcgat tttgatgctc
      841 tacaaccaaa atggatagta ggttattcag atattagtac gttgctactt gctgtcacat
      901 taaaaacagg gatagccact gcacatggaa ctaaccttat tgatttaaga ggagagtact
      961 tggatgaaac gacagcaatg tgggaaagtg tactgaaaac aagagaagga gaatcgattc
     1021 tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca tgagcattca tcatcttctg
     1081 ttttccatct cactgaacca acaaattggc aatcagtcag cggagatgca gtaaggatga
     1141 agggacgatt attaggtggt tgtattgata cgatcaggca tttaatcggt actccttatg
     1201 gagatgtcca atcttttaaa agaaagtata atgatggaaa gccgatcttg tggtttctag
     1261 aaaactgtga gctatcgtca gttgatttaa gaagatcact tgttcagatg aaactggcag
     1321 gctggtttga tggcagctcg gggatcatgt tcggtagaag tgcggcagag gatttgttag
     1381 attataaaat gaaggatgta tacgaagatc tgtcaaagga attaaaacta ccgatcttgt
     1441 atgatattga ttgtggtcat gtaccgccac aactgacatt gattaatggt gcctatgcag
     1501 aagtagcggt agaagataga aaagcgactg ttttgcaaac gtttatttaa aaaatttttt
     1561 cctgccttgg agtctatcaa agaccaataa atctaaatag aaaagaaagt gaaagaagct
     1621 gacaaaatgc tgtcagcttc tttttgtata acatattaga aagtctaatt atctataact
     1681 gtcacatcga ttaactgttt cacgtcattg ggatttcctg taggaatagc tactacagat
     1741 gcattatcaa tgatttcttg agttgtttct ctaatgaacg ctggataaga tgtttgatct
     1801 gcaggcattt gccaatgtga aggaactgga taactagtga ttgtcccgtc atggttactt
     1861 gaatacacta aaactccctg gtaactgtat ttcccagtga gcataaccac atctttcgga
     1921 tagactgcac tccctgcatc ataaggggcg ataggtgttc ctgcaggata atgaataaca
     1981 tgtaattcga aatcacccga tgtatctcta aaagtattac ctctaatagc tagccaaaca
     2041 cgtgcgtact caatttgtag atcagaatat cctgctaacg gatctattga ctgagaagac
     2101 tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg tctcaactgt actactagat
     2161 gtagaagaag tattgggctc tgagacttta ctgcttgttg aagatgtatt cgtttcagta
     2221 gaagctgatt tctgagtact tgatttttca gctgaactgg tttgtctgat tgaacttttg
     2281 tctgtggtcg cagatggatc actattttct ttactaggag acgtacatcc tacaaggaat
     2341 aaagagacca acaataaaat aaaacctaac tttttcatat aaaatacctc attatttttt
     2401 taataccagt atagcataat cctgaatttt tgaatccgta aaaccttgtc tatgaatata
     2461 aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataat catcagtaaa
     2521 attacttgtc acactattga taaggagtat ttaattgaag agaatgatta ggagaaagga
     2581 tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat cgttttttct
     2641 cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa ttctatagga
     2701 gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc tataaaatct
     2761 tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc ggtgtgtttg
     2821 taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat agcagctact
     2881 ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt ttttatgtcc
     2941 catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg ttcgattaag
     3001 taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat agaagcagtt
     3061 ttgttttgcc atttctcatt ttggtttagt gtgctagtaa tataagcacc ttcccaatcg
     3121 gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc ctttactatc
     3181 tataacaagg acaacagttt taacctacag acattcatta aaattgggta gcgacagcga
     3241 aaacaacaat tcttaagttt tagacagagt atacagatag tcaacttaat taaatacgaa
     3301 ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt ttgcatacat
     3361 ggatgacaaa agaaccaatt cccacttgct tgagtacaat attaaaagaa actggaaggc
     3421 aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat gaaaacaacc
     3481 ctagagatat ataactaggt aaccagttta atgaaagaaa aaatcatcca agaagtcgat
     3541 tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat atgagaggtt
     3601 gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata gcttttcata
     3661 tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt tatcgagagg
     3721 ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag ctaggtttcc
     3781 ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat tttaagaagg
     3841 tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata gaaacaataa
     3901 atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag catatctgct
     3961 attactgtga ttttaataaa gtttttttag aagggcagcc agtagatgag tatgtggaaa
     4021 tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc gacagcgaat ttagaaacac
     4081 tttacgtagg gggcgggaca ccaacttctt tatctgctaa acaacttgat cgactgttat
     4141 caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta gaagcgaatc
     4201 caggtgattt gacccgtgaa aaacttcaag taatgaaaaa ctatggcgtc aatcgtttat
     4261 ccatgggagt gcagacattt gataatcgct tattgaaaaa aattggacgt aaacacaccg
     4321 cagaagatgt gtatcaaact atgcaatttt tagaagcaga aaattttacc aatgtaagca
     4381 tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatctggat actttggaaa
     4441 aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc gaaaacaaaa
     4501 caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag gaaacggaaa
     4561 ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac caatatgaaa
     4621 taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac tggaacaatg
     4681 accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt cgttaccgca
     4741 acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc ccggtattgg
     4801 aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta ggactaagaa
     4861 aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca attgaatcgg
     4921 tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat gaagaagacc
     4981 gtgtttattt gacaccaaga gggacctttt tggggaacaa tgtgtttgag cgctttttgt
     5041 ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat ttaaataaaa aaagttcaaa
     5101 aatacaataa aggactattg aaacgttgat ataactggat ttacatcaat tatttagaat
     5161 tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct
     5221 tcttgctata tttataagtg gattagcact tagaatatat gagtgctaat gagaggtgag
     5281 gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag
     5341 tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag ggagtcaagg ccagctcagc
     5401 aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc
     5461 ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa
     5521 acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt
     5581 tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta
     5641 tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt
     5701 acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag
     5761 ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat
     5821 tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga
     5881 gattccgatg attcttcata aatatttcca aacgacagaa gggatgtctc agttatttga
     5941 caccgttctt ggtcatgttt ttgaagataa ggtttatgtc agtggacaaa tgaatctctt
     6001 ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga
     6061 ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg
     6121 tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat
     6181 agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg
     6241 gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta
     6301 ccgttcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt
     6361 tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat
     6421 cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga
     6481 aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga
     6541 aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc
     6601 tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg
     6661 ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc
     6721 cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga
     6781 aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc
     6841 ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc
     6901 cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc
     6961 aaccatggtc attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag
     7021 aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac taactctgca
     7081 gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca
     7141 acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt
     7201 caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt
     7261 tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt
     7321 caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc
     7381 actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc
     7441 gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt
     7501 ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt
     7561 gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac
     7621 aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc
     7681 aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac
     7741 gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca
     7801 tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa
     7861 tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgcta
     7921 aaagatgcag gtttatcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact
     7981 cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca
     8041 gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt
     8101 gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg
     8161 ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa
     8221 gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa
     8281 cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca
     8341 gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta
     8401 aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct
     8461 tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag atgcagaagc aaatgcagaa
     8521 gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagatgc attgctattc
     8581 tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa
     8641 gcagaaacag ctcgcgatga attgaaagcc gctgttgaag caaacaacat cgaagaaatg
     8701 aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa
     8761 caagcggcac aacaacaagc tcaagaaaac ccagaagcag ctcaaggcgg agcagatgat
     8821 gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt
     8881 tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc
     8941 ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca
     9001 ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa
     9061 ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct
     9121 gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag
     9181 attttaagtg acccgcaaaa acgcgctgca tatgaccaat acgggcatgc aggcacagat
     9241 ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt
     9301 ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca
     9361 gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt
     9421 gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat
     9481 acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat
     9541 ggtgcaggta cgattaatgt agaacgccaa acaccacttg gacgagtgat gagccgtcaa
     9601 acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag aaccttgtcc aacctgtcat
     9661 ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa
     9721 gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt
     9781 ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata tctttgatcg tgaaggttct
     9841 gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa
     9901 gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg
     9961 aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa
    10021 catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta
    10081 cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc
    10141 gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaacgc
    10201 taagtcatta ataattaaaa accagacgaa caatcatatg ttcgcctggt ttttttgtag
    10261 ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt catagtttct
    10321 aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg tgtttttatg
    10381 aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga agcatgccaa
    10441 agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac gttttacata
    10501 taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta aatcttcaaa
    10561 aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt gtttggcatg
    10621 aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc caaagtttag
    10681 ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg atagcaatga
    10741 ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag tgagcaaagc
    10801 ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga tccgttcttg
    10861 acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag aagaaaaatc
    10921 aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat gaaccgatgg
    10981 tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga aaacaagcga
    11041 tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc ccttcatggc
    11101 gaccgtgcat ttttggtttt tattaaatat ttaatacttt attcaagaaa tcttttgtcc
    11161 gttcattttg cggattttca aagatttgtt gaggcgttcc atcttctaaa aagtttccgt
    11221 cgtcaatgaa catgacgcga ttggccactt ctttagcgaa ccccatttcg tgcgtaacga
    11281 taaccatcgt cataccttgt tctgctaact ttttcatgac gttcaagaca tcaccgacca
    11341 tttcaggatc gagtgcagaa gtaggctcat caaaaagcat gatgtcaggg ttcatcgcta
    11401 gagcacgagc aatagccaca cgctgttttt gtccaccaga aagcatttca ggatacatat
    11461 cttttttatc ttctagacca actgtctgca gtaatttgat tgcttttttc tctgcttctt
    11521 ctctagattc tttcttcagg tccatcggtg ctaaaatgat gttttccaaa atagaaagat
    11581 gagggaatag gttaaagtgt tggaatacca ttccgatatg ttgtctcacc aagttgatat
    11641 ctgtgctttt atccatcaag tgggcgccgt caataataat ttcaccgtca gaaggttctt
    11701 ctaaacgatt caagcaacgc aagaatgtac tttttcctga acctgaaggg ccaatgatac
    11761 agacgacatc tccttcgttg atggatacat tgatgtcgtt taatactgtg ttatcgccgt
    11821 attttttgac taaatgctca actaagattt tttctgccat cttacttcac cttcttttct
    11881 aaaacttttg ctaattttgt caatgctgtg atcaaaatca agtacatgat cgcaatgatc
    11941 aagtaaacat acgtactttg tgttgtacgt gcaacaatga tcttaccagt ttgcagcaat
    12001 tcgactacac cgattgcgga aatgatcgtt gtgtctttta atgagataac gaactgattg
    12061 acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa tgattttttg cattgtccga
    12121 ttataagaaa gacctaaact tctggaagct tccatttgtc cgacaggaac ggcgttgatc
    12181 ccgcctcgaa caatctctgc gatataagca cttgcgttaa gagtcaaggt gataacacca
    12241 gcaaggaagt caggaatcgt aaatccaacg ataccaggca acccaaagaa aataaagaac
    12301 gcaagaacca tcattggaat tcctcggatg atatcgacat aaatactagc aattgtgcga
    12361 agagcgcgta ttggtgctac actgaataat ccaaaaatca caccgattac tatagccaaa
    12421 gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc ctttcaacaa tgcttgatag
    12481 ttgttttgga taagtccaag aaatgttgat tcatcagcag tggaagactc actggaagtt
    12541 tctgaaccat ccgctacata ttctttaata atcttatcgt attcgcctgt tctttttaat
    12601 tctttcaatc cttcattgaa catattaaga agctcaggat tttgtccttt tttaacggca
    12661 aatgcatatt ttccgccaga ttcttttttg atcggtgttt ccaattcttg tccttgtttg
    12721 atcgcataac cgattaccgg ataatcatcc atcattgcat cgattgcgcc aacttttaac
    12781 ccgtcgtaca attgatcagc agcatcaaaa tatttgatag aatagccata ttcagattca
    12841 tggtcgctta aaaattcagc actttcggta ccaacttttg ctccgactgt ttttcctttc
    12901 aaatcttcat aagacttgat cgaagtatct ccttttttga cagctaattg gatcccgcta
    12961 tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt catctgttac agtcattccg
    13021 gcaatcattc cgtctgcttg tcctgattcc aaagcttgca aagcagcaga gaaaccacgg
    13081 tagtccattc ggatgttaaa tccttgcagt ttggcgatgt tttccatcaa ttttacatcg
    13141 atgccttcgt actcgccctt tgcgttttga aattcaaatg gagcaaaagc agagtcactg
    13201 gcaattacgt atacgtcttt tttgggctct attttttgca ttgtctcgct tgtgcttgac
    13261 tctgccgttg tagtgttgtc agcttgtgca acatgaccaa acgcaaatgt cacaagtcct
    13321 gtcattaaga aaacaaatga gtgaaataaa tgctttcttt tcataatcaa cctcctaaat
    13381 ttccaatatt ttattattat aggcgaaaag tgctgaatca gacaagactg taagctcgaa
    13441 aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata aaacataaca tgtataataa
    13501 gataaaaaag tggcgaaagt atgttcggtg tggtataatt ttttagtaag gaatgccttt
    13561 gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa tgaatgaaaa ggaggacgaa
    13621 gaatgattga gagagatacc tcccgacact ttgagcttgt ctcgaaatac caaccggctg
    13681 gtgatcagcc tgaagcgatt aaccaattag tggatggcgt agtcggcggg aaaaaagcac
    13741 aaatcttatt aggtgcgact ggaacaggga aaacatatac gatttccaat cttattgaga
    13801 aagtgaataa accaacactg attattgccc ataacaaaac attggctggt cagctgtacg
    13861 gggaattcaa ggaatttttc cctaacaatg ctgttgaata ttttgtttct tattatgatt
    13921 attatcagcc tgaagcttat gtgccttcaa gcgatacgta tatcgaaaaa gattcaagtg
    13981 taaatgatga gatcgacaaa ttacgacatt cggctactag ttcattatta gaaagaaatg
    14041 atgtgatcgt cattgcatcc gtttcatgta tttttggatt aggctcgcct tttgagtacc
    14101 aaaaacaagt agtttcgatt cgacagggag cagaactaga tcgcaatcag ttgattcgtg
    14161 acttagtcag tatccaattc gaaagaaatg atatagattt tcagcgcggt cgttttcgag
    14221 tgcgcggtga tgtcgtggag atttttccgg cttcaagaga tgaacgtgcc ttacgtgtgg
    14281 agttttttgg agatgaggtc gaacgtatcc gagaagtcaa tgcactgaca ggtgaagttt
    14341 taggagaaac ggaacacgta gcaatcttcc cggcgactca ctttgtgacg aatgatgaac
    14401 atatggagca tgccgtagcg aatatcaaag cggaattaga acagcgttta actgtcttgc
    14461 gaaatgaaaa caaacttctg gaagctcaac gtttagaaca gcgaaccaat tacgatattg
    14521 aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa ttattctcgg catatggacg
    14581 gacgtaaaga aggtgaaccg ccttatacgc tattggattt ctttcctgaa gattttttga
    14641 tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag aggaatgtat aatggtgacc
    14701 gagcgagaaa acagatgcta gtagattacg gtttccgttt gccatctgct ttagacaatc
    14761 ggccacttcg gttagaagag tttgaaaaac atgtcaatca gatcatttat gtctctgcga
    14821 cgccaggacc atatgaacat gaacaaacag ataccgttat ccaacaaatc atccgtccga
    14881 cagggctgct tgacccagtg atcgaagttc gtccaatcat gggacaaatc gatgatctag
    14941 taggcgaaat aaatgaacga gtcgaaaaag accagcgggt gtttgtaacg acattgacga
    15001 aaaaaatggc agaagacctc acagattatt tcaaagaatt agggatcaaa gtcaaatatt
    15061 tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat tcgagatctt cgattaggtg
    15121 aatttgatgt gttagttgga atcaatttgc ttcgtgaagg attagacgta cctgaagttt
    15181 cccttgttgc tatcttggat gcggataaag aaggcttttt acgtagtgaa cgttccttag
    15241 tccaaacgat tggacgagct gctcggaatg aagaaggtaa agtaatcatg tacgcagaca
    15301 aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc aagacgtcgt acgattcagc
    15361 agaagtacaa tgaagaacat gggatcgttc caaaaacgat cattaaagaa atccgagatt
    15421 tgatttctat tacgaaagaa tcggaagatg atacaaaaga agcggtacaa gtctcgtacg
    15481 aagaaatgac gaaggaagaa aaagacacgt tattgatgaa attagaaaaa gaaatgaaag
    15541 atgcggcaaa agctttggat tttgaaacag ccgcgaatgt gcgagatatg attttagaat
    15601 taaaagcttc aaagtaggag gacaacatgg ctaacgataa aattacgatt catggtgcac
    15661 gtgcccataa cttgaaaaat atagatgtaa cgattccacg agataaattc gtggtcgtta
    15721 caggtttgtc cggttctgga aaaagttccc tagcatttga tacgctgtac gctgaagggc
    15781 agcggagata cgtagagagc ctttctgctt atgctcgtca gtttttagga caaatggata
    15841 agccggacgt agatagcatc gacggattga gtccggcgat ttctattgat cagaaaacaa
    15901 caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga aatcaatgac tatttacgct
    15961 tgttgtttgc acgtgtagga catccgattt gtcccaacga tcatatcgag attaccagtc
    16021 aatcagtaga tcaaatggca gatcaagtaa tggctttgcc tgagcgcaca aagatccaag
    16081 tgttggcacc gatcgtggta aagaaaaaag gacaacataa aaaaatattc gaacgaatcc
    16141 aaaaagaggg atacgtccgc gttcgagtcg atggtgagat ctatgattta agtgaagtgc
    16201 ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt catcgaccgg atcattgtga
    16261 aagaagggat tcgttctcgc ctatttgatt ctttagaggc ggccctgcga ttagcagacg
    16321 gctatgctct ggttgacgtg atcggtgaag aagaaatgtt gtttagcgaa cactatgcct
    16381 gtccttattg cggatttact gttggcgaac tagaaccaag actgttctca ttcaatgcgc
    16441 cttttggagc ttgtccagac tgtgatggtt taggtgtcaa attagaagtc gatcaagatt
    16501 tagtgattcc agatagaacg aagacactta gtcaaggtgc tatcgttcca tggaatccaa
    16561 tcagctctca gtattatcct caaatgttag cgcaggcttg tgaaagtttc ggtatcgatt
    16621 tagatacacc attcgaagaa ttaccggaag agcatcagga gatcatcttg aatgggacag
    16681 atgatctttt ccatttccat tatgaaaatg attttggtgg cgttcgcgat gtagaaacga
    16741 ctttcgaagg ggtattgaag aatatcgagc gtcgttatca tgatacgaac agtgatttca
    16801 cgcgtgagca aatgcgtctt tatatgacag aattaacttg tcagacatgt aaaggttatc
    16861 gattgaatcc ccaagctcta tctgttcaaa tcaatggaac gaatatcggg caaacaaatg
    16921 agttgtctat tcaaaaagca gtagatttct tctcaaactt aacgttatct gaacaagaga
    16981 aagtaattgc caaaccgatt ttaaaagaag taaatgacag actttcattc ttggaaaatg
    17041 tgggactaga ctatctaact ttgagtcgcg cttccggaac attatctggg ggagaagcgc
    17101 agcgaatcag attggctaca caaattggat cgaatctttc tggagttttg tatattttag
    17161 atgaaccatc gatcggattg catcaacgcg acaacgatcg actgattggc tcattaaaga
    17221 aaatgcgtga tcttggcaac accctcgttg tagtcgaaca tgatgaagat acgatgtggg
    17281 cagcagatta tttgattgac gtcggacctg gggccggaga acaaggcgga caaatcgtgg
    17341 ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc tttgacaggg aaatatcttt
    17401 cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa aggtaatggg aaagcaatca
    17461 agatcactgg tgcttcagaa aacaatctga aaaatattag tgttgaattt ccattaggag
    17521 aatttgtagc agtcacaggt gtgtctggtt ctggaaagag tacattggtc aatagtatct
    17581 taaagaaatc gttagcgcaa aaattaaata agaattctgc taagccaggt aaattcaaga
    17641 caatttccgg ctacgaaagt atcgaaaaga tcatcgatat cgatcaaagc ccaatcggcc
    17701 ggacgccgag aagtaatcca gcgacttata caagtgtatt tgatgatatc cgtgggttat
    17761 ttgctcaaac gaacgaggca aaaatgcggg gttataagaa agggcgtttt agtttcaacg
    17821 taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat tattaagatc gaaatgcact
    17881 ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg taaacgatat aattctgaaa
    17941 cattagaagt gcattacaaa ggaaaaagca ttgctgatat tttggaaatg acagtagagg
    18001 atgctgtaga attcttcaag cacattccaa agattcatcg caaactgcaa acgattgttg
    18061 atgttggctt aggttatgtg acgatggggc aaccagcaac gacattgtcc ggtggtgagg
    18121 cacaacggat gaaacttgcc agtgaattgc acaaaatctc taatggaaag aatttctata
    18181 tactagatga accaacgaca ggacttcata gcgatgacat cgcccgcttg ttgcatgtat
    18241 tacaaagatt agtagatgct ggtaacacag ttttagtgat tgaacacaat ctagatgtaa
    18301 tcaaaacagc agattatatc attgatttag gaccagaagg tggagaaggt ggaggaacga
    18361 tccttgcgac tggaacacca gaagaaatca ttaacgtaaa agaaagttat acaggtcact
    18421 atttgaaaaa aataatggta taaagaggat agatagctga gatagttaaa aaaacataaa
    18481 aaaatgaatt tttgcttgtt ttattaatat cagtgttcta gaatcattac atggtagttc
    18541 tttctacact gattggtata gataaaaact tcctataagt tcaatccgtt caaaaaagaa
    18601 ttgagcaatc ggaggacttt cctttgattg cttttttctt tatggtatga tattccttgt
    18661 gtagttggaa aagaaaaaca agagattatg agtaagtgag agataatgat gaacagtatt
    18721 gaaaatgaag aatttatccc tatattatta ggaagcgaca tgaacgttta tggtatggca
    18781 cgttctttta atgaagcata cggcaaaatc tgccaagcat atgcttcgga tcagttagcc
    18841 cctacccgct acagtaaaat tgtcaatgta gaagttattc caggatttga taaagaccct
    18901 gtatttatcg agacgatgct tcgtttggca aaagaaagat attctgataa gagtaaaaaa
    18961 tatcttttga ttgcttgtgg tgatggatat gcagaattga tctcccaaca taaacaagag
    19021 ctgtcagaat attttatctg tccatatatt gattattctt tatttgaaag gctgatcaat
    19081 aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc cgtatcctaa aacattgatc
    19141 gtcagagaag aaatgttagt aaatggacat ttggaacagg aattgccttt tgaattccca
    19201 gttgctttga aacctgcaaa cagtgtggag tatctttctg tccaatttga aggaagaaaa
    19261 aaagctttta ttttagagac tagagaagag ttcgatttaa ttttaggccg gatatacgaa
    19321 gccggttata aaagtgaaat gattgtacaa gattttatcc cgggtgatga tagtaatatg
    19381 cgtgtgctaa atgcgtatgt agatgaagat catcaagttc ggatgatgtg ccttggtcat
    19441 ccactattag aagatcctac accagcatcg attggtaatt acgttgtgat catgccagat
    19501 tataacgaaa aaatctatca aaccattaag gcatttttgg aaaaaatcga atatacaggc
    19561 tttgccaatt tcgatatgaa gtatgatcct agagatggag aatataaatt atttgaaatc
    19621 aatttgagac aaggtcgtag cagtttcttt gtgacattga acggcttgaa tctggctcgt
    19681 tttgtcacag aagatcgagt gttcaacaaa ccctttgttg aaacgaccta tggaacgaat
    19741 caatcagata aagcaagatt atggatgggt gtgcccaaaa aaatcttttt agaatatgct
    19801 cgggaaaatg aagacaaaaa actagctgag caaatgatca aagaaaatcg ttacggtact
    19861 actgtatttt atgaaaaaga ccgatcaatc aagcgctggt tgttgatgaa atatatgttc
    19921 cataactata ttccaagatt caaaaagtat tttcatgtga aagaaggcta gttcatggag
    19981 aattttttca gtattttagg cggaatgggc acgatggcga cagaaagttt tgttcgcttg
    20041 atcaaccacc gaacgaaagc tacaaaagat caagaatatt tgaattatgt cttattcaat
    20101 catgcaacag ttcctgatcg tacagcttat attttagatc gatcagaaga aaatccaatg
    20161 ccgtttctgc tggatgatat tgagaaacaa aatttattgc gaccaaactt cattgtgttg
    20221 acttgtaaca ccgcacacta cttttttgaa gagttacaag cggcaacgga tattcctatc
    20281 ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc agcatacgac agggagagta
    20341 gcgattctag gaacagaagg aagtatgaaa gctggaatct atgaacgcga agtcaaaaat
    20401 cttggtttcg aaacagtgat tcctgatact gcgcttcaag aaaaaatcaa ttacctgatc
    20461 tatcatgaga tcaaggaatc ggattatttg aatcaagagc tctattacga aatcttagag
    20521 gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag gttgcacaga attatctttg
    20581 atgcatgaat ttgctgaaga taatcattac ccagtgatcg atgctcaatc tattttagcg
    20641 gatcgaacga tcgaacgtgc attagctgaa agaagcgagg cattggatac agcatcggaa
    20701 aagtaaaaaa attggatttg gggcaaatat cctaaaagat atttggtcca aatctttttg
    20761 taaaagaaaa agaaaactgg taatgatacg cccgggatga tataatttta ttatgcacaa
    20821 tgtgagaggg gttcgtaaat atgacagata atttgcaatt agtcatcata acaggtatga
    20881 gtggtgcagg gaaaacagta gctatccaaa gttttgaaga tatgggctat ttttgtatcg
    20941 ataatatgcc gcctcgattg attccaaaat tctgggaatt gatcaaagaa tcaggtaaag
    21001 tcacaaaaat ggctttagga gtggatttgc gttctcgttc cttctttgaa gaaatccaaa
    21061 acatgctgat cgaaatcgaa aatacgaact tcatcgacac ttctatctta tttttagatg
    21121 cttcagacga agaactagtg gctcgctaca aagaaacaag acgagcacat ccaatggcga
    21181 tggacggttt gatcactgaa gggatacgta aagaacgggc gatactagac gacttgaaag
    21241 cccaagcttc cgtgatcatt gatacgacaa cattgacacc acgacaatta cgtgaaaaaa
    21301 tcaatgagtc attcaaatca aagggagacc aaggatttcg gatcgaattt gtatcatttg
    21361 gttttaaata cgggttgccg atcgattcgg atattgtgat ggatgtgcgt tttttgccca
    21421 accctcatta tatcccggaa ctaagaccat tgaccggaaa agatcagcca gtctatgatt
    21481 atgtgatgtc ctttgatgaa acgcaatcat tctaccataa atttttggat cttctggaaa
    21541 ctgtattgcc tggatacatt aaagaaggaa aaagcagctt gacagttgca atcggctgta
    21601 caggcggtca acaccgttct gtggcattga ctgaacgttt ggcaaatgct ttagggaaaa
    21661 actataaagt caatgtgaca catcgggaca aggataaaag gaaagagacg gtgaaccggt
    21721 cgtgaaaatg aaaacctacc ggataagaaa accaaagatc gtagtggtcg gcggtggcac
    21781 tggtctgcca gttatcctga aaagtttacg aaatcaagga gcagatatca cagcagtagt
    21841 gactgttgca gatgatggtg gaagcagcgg tgcaatcaga gaatcgatcg ctatggctcc
    21901 tcctggtgac ttgagaaatg tcttagtggc gttatcggat atgccacaat tctatgaaga
    21961 tattttccaa taccgtttca aaaaggaaga tcagttttta gccaatcata cgatcggtaa
    22021 tctgatcatt gccgcagttt ccgagatgcg gggaagcact tatgaggcga ttcaattatt
    22081 agcaaaaatg atgcatgtag atggacatgt ttatccttct tcagaaactc ctttgacctt
    22141 gcatgctgtt tttaaagacg gaacgactgc agtaggagaa tctaaaatcg cgatcaaccg
    22201 taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat aaaagtgaag ccaaagcggc
    22261 tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc gttttgggac ccggaagttt
    22321 attcaccagt atccttccaa atttagtgat accagaaatc ggcgaagcaa tgctcaagac
    22381 aactgctgaa accgtctata tctgtaatat catgacccaa aaaggggaaa cggaacattt
    22441 cacagatgcc gatcatatac gtgttttaca caagcatttg aatcagcctt ttatcgatac
    22501 agtattggtc aatacggaaa aagtgcctga agattatatg gacccagaga tctatgatga
    22561 gtatctagta caagtcgcac acgacttcaa cggattgaga aatgaaggat gtcgagtgat
    22621 ttccaccgac tttttagaat tgaaagatgg tggtgtcttc cacgatggag acaaagtagt
    22681 agaagaattg ttccgtttag ttttcggttc aaaatattaa gcaatttatt gttggagaaa
    22741 ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa gaactaactg gtttggaagt
    22801 tcatcgagaa catgcgaaag cggaattagc tgctttgctg cgaatgaacg gctctttagg
    22861 tattgtgaac cagcagttta ttttgaacgt ccaaacagaa aatgctgcaa ttgccagacg
    22921 tatctattct cttttgaaag atcattatca ggtacgaagt gagctactcg ttcgtaaaaa
    22981 aatgaaatta aaaaagaaca atgtctatat cgtccggttg aagcaaggca caaaagatgt
    23041 gttgatggat cttgatatca tggatggtat gatgttcaat ggacacgttt cggacgagat
    23101 tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt gcttttatgg cctcaggttc
    23161 agtcaataat ccggaaacga gtcgttatca tttggaaata ttttctattt atgaacagca
    23221 taaccaagac atttgcgata tgttaaatta ttacggtcta catgctcgaa cgcttgaacg
    23281 gcgcaatggc tatatttcgt atttaaaagg agcagaaaaa attgctgatt tcttaacatt
    23341 gattggtgca accaactcaa tgctccgatt tgaagatgtt cgaatcgtac gggatatgag
    23401 aaattcggtc aatcgtctag tcaattgtga gacagcaaat ctgaacaaga cgattgatgc
    23461 ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca aaggtcggtc tacatgccct
    23521 tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa catccagagg tcagcttgaa
    23581 agaattaggg gaaatgattc cctcaggtgc aatatcaaaa tcgggaatta atcaccgaat
    23641 tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa gcagtttaat ccaaagaaaa
    23701 aactgataca tgaagcagga gatgcgaatg tatcagtttt ttttaaaagc tatacttgct
    23761 tttcttccgt caaactataa ttccaacttt actggagtaa cataatgttg aaaggcttct
    23821 attgtttttt aatacaatcg tataaaaagc ggggagacaa ctgaatgaag attttagttt
    23881 tatacataaa cttttgctta atatagaagt cctgatatag ataggatcaa gtatttatct
    23941 ataactggag attaattaaa aactggaagg aactgctcga tcaaccttga gaactgtttt
    24001 tcttacttct ggtcaagatt ctcgtggttg gtggataagg atggttgata agttcattgg
    24061 attagctagt cgaaaacaac caggtgtaat taatgtagaa gttttaacaa ggggatggag
    24121 tgatttattc agtgataatc ataagttcat aaatagtaat tgggatgtat ggttggtata
    24181 cgttatacct gaacttgaac tatcaagatt tggtttagaa tttgagaatc gatgtttata
    24241 aagggagggc aaaaaatgtg cttttttaca acttttacta tcattatgat tgcagtactt
    24301 tttatttttc tagatatagc aaagcgaaat acagcatttt tattatatcg ggttttactt
    24361 cgagcaggtc tcataacatt tatatcgatc gttggatttt ttctattcac agttattgta
    24421 tttatttgga gaactacagc accaccattg ccagagatta catatggaga atttccgttt
    24481 agtcttgaat atgaaataga tgaggaaaga attttgattg aagatacggt gattgcagaa
    24541 tttataggaa gttttagagg gaatgtgaca actcaaccta gacgaagttg gagtataaaa
    24601 cttttaaata atagtggaga gttgaaagca tttcattatg aattcttaat aatagaaagt
    24661 gaagatgtaa caatcacttt aagtagtggc tctgctggat attttatgaa tgatggtgga
    24721 agcaagccac agctcacagt ccgtgatcgt attgcaggtt ttatgcattt ttctcactat
    24781 gacttagaag aagctcgtaa aatacttgca gaatatggga tcacattaat caattgggaa
    24841 tatgcgccac cgataagaaa ttttttcaat gattattatt agtcttataa aaaacagaat
    24901 gtaaaattta tgtgcgcgag tatttagttt tccatttttt gtttgataga tatatagcat
    24961 tgttgaagca tgggatagtg gtgccaattt tatcaatgag gttggtcagg cagttaacat
    25021 aattggtgat gctataaatc catttaattg gaattggagg aattaggtgt ggaaacagtg
    25081 agattatgga tattcatgaa aaattttcgt tcggctttga gatatgttat tctggaatca
    25141 aaatcaggtt attatctgat tgacacagat cactatactt tttggggagg ctaccttttt
    25201 ccgattttta attggataat cccccataag gttatgaaaa ttaacttgcc tgcagaagaa
    25261 ataaatagat taatactcaa caaagattta caaaggaaaa gagagaaaga acattctaat
    25321 gttgcttgga taggtccagc atttgcagta gtgacttctt caattgtcgg tcctatgcta
    25381 gagagtattt atagtgattt ttctattgga atacgttatt tatttgttac tttaagtata
    25441 gctgttcttt tgtgcataaa gggttatgtt agcaagaaga ataaagatat tttaacacta
    25501 atcggtgtaa acaatttatc tagtactaaa attatactgt ttcctgaatc tataaaagaa
    25561 ggatcaaggt tgttgataat ttttataatg cttatactgt tttctgtttt atttggggtg
    25621 atgtttata
//
LOCUS       DAZAOG010000019        24980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000019 DAZAOG010000000
VERSION     DAZAOG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.019"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1233)
                     /locus_tag="ACHDLD_000745"
     CDS             complement(451..1233)
                     /locus_tag="ACHDLD_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362499.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="ACHDLD_000746"
     CDS             complement(1298..2134)
                     /locus_tag="ACHDLD_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3362500.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="ACHDLD_000747"
     CDS             complement(2152..3561)
                     /locus_tag="ACHDLD_000747"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3362501.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="ACHDLD_000748"
     CDS             complement(3575..5398)
                     /locus_tag="ACHDLD_000748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3362502.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="ACHDLD_000749"
     CDS             complement(6241..6567)
                     /locus_tag="ACHDLD_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362503.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="ACHDLD_000750"
     CDS             complement(6613..6975)
                     /locus_tag="ACHDLD_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN3362504.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="ACHDLD_000751"
     CDS             complement(7012..7227)
                     /locus_tag="ACHDLD_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3362505.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="ACHDLD_000752"
     CDS             7354..7689
                     /locus_tag="ACHDLD_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3362506.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="ACHDLD_000753"
     CDS             complement(7765..9762)
                     /locus_tag="ACHDLD_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362507.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="ACHDLD_000754"
     CDS             complement(9860..10135)
                     /locus_tag="ACHDLD_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362508.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="ACHDLD_000755"
     CDS             10367..11101
                     /locus_tag="ACHDLD_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3362509.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="ACHDLD_000756"
     CDS             11140..12282
                     /locus_tag="ACHDLD_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3362510.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHDLD_000757"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHDLD_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN3362511.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHDLD_000758"
     CDS             complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHDLD_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN3362512.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="ACHDLD_000759"
     CDS             complement(14230..15465)
                     /locus_tag="ACHDLD_000759"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN3362513.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHDLD_000760"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHDLD_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN3362514.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHDLD_000761"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHDLD_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN3362515.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="ACHDLD_000762"
     CDS             complement(17731..18579)
                     /locus_tag="ACHDLD_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3362516.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="ACHDLD_000763"
     CDS             complement(18603..19289)
                     /locus_tag="ACHDLD_000763"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN3362517.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="ACHDLD_000764"
     CDS             complement(19282..20316)
                     /locus_tag="ACHDLD_000764"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN3362518.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="ACHDLD_000765"
     CDS             complement(20711..21049)
                     /locus_tag="ACHDLD_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN3362519.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="ACHDLD_000766"
     CDS             complement(21051..21407)
                     /locus_tag="ACHDLD_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN3362520.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="ACHDLD_000767"
     CDS             complement(21413..22600)
                     /locus_tag="ACHDLD_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN3362521.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="ACHDLD_000768"
     CDS             complement(22791..23636)
                     /locus_tag="ACHDLD_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3362522.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="ACHDLD_000769"
     CDS             23870..24835
                     /locus_tag="ACHDLD_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN3362523.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7309 a         5247 c         4241 g         8183 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa
//
LOCUS       DAZAOG010000020        24630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000020 DAZAOG010000000
VERSION     DAZAOG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.020"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHDLD_000770"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHDLD_000770"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN3362524.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHDLD_000771"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHDLD_000771"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN3362525.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHDLD_000772"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHDLD_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN3362526.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHDLD_000773"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHDLD_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN3362527.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHDLD_000774"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHDLD_000774"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN3362528.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHDLD_000775"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHDLD_000775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN3362529.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHDLD_000776"
     CDS             complement(8053..8523)
                     /locus_tag="ACHDLD_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN3362530.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHDLD_000777"
     CDS             complement(8569..8994)
                     /locus_tag="ACHDLD_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN3362531.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHDLD_000778"
     CDS             complement(9061..9330)
                     /locus_tag="ACHDLD_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN3362532.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHDLD_000779"
     CDS             complement(9341..10936)
                     /locus_tag="ACHDLD_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3362533.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHDLD_000780"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHDLD_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN3362534.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHDLD_000781"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHDLD_000781"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN3362535.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHDLD_000782"
     CDS             15402..16376
                     /locus_tag="ACHDLD_000782"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN3362536.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHDLD_000783"
     CDS             16546..17946
                     /locus_tag="ACHDLD_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN3362537.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHDLD_000784"
     CDS             complement(18024..18746)
                     /locus_tag="ACHDLD_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362538.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHDLD_000785"
     CDS             complement(18825..19331)
                     /locus_tag="ACHDLD_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3362539.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHDLD_000786"
     CDS             19454..21253
                     /locus_tag="ACHDLD_000786"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN3362540.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHDLD_000787"
     CDS             complement(21321..22895)
                     /locus_tag="ACHDLD_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN3362541.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHDLD_000788"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHDLD_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN3362542.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHDLD_000789"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHDLD_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN3362543.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
BASE COUNT         6806 a         5352 c         4472 g         8000 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc
//
LOCUS       DAZAOG010000021        24386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000021 DAZAOG010000000
VERSION     DAZAOG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.021"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..911)
                     /locus_tag="ACHDLD_000790"
     CDS             complement(63..911)
                     /locus_tag="ACHDLD_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3362544.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACHDLD_000791"
     CDS             complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACHDLD_000791"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN3362545.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3412..3591)
                     /locus_tag="ACHDLD_000792"
     CDS             complement(3412..3591)
                     /locus_tag="ACHDLD_000792"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN3362546.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3638..5461)
                     /locus_tag="ACHDLD_000793"
     CDS             complement(3638..5461)
                     /locus_tag="ACHDLD_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3362547.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5739..8018
                     /gene="helD"
                     /locus_tag="ACHDLD_000794"
     CDS             5739..8018
                     /gene="helD"
                     /locus_tag="ACHDLD_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN3362548.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8111..8758)
                     /locus_tag="ACHDLD_000795"
     CDS             complement(8111..8758)
                     /locus_tag="ACHDLD_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362549.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8937..9656)
                     /locus_tag="ACHDLD_000796"
     CDS             complement(8937..9656)
                     /locus_tag="ACHDLD_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362550.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9787..10938)
                     /locus_tag="ACHDLD_000797"
     CDS             complement(9787..10938)
                     /locus_tag="ACHDLD_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN3362551.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11155..11895)
                     /locus_tag="ACHDLD_000798"
     CDS             complement(11155..11895)
                     /locus_tag="ACHDLD_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3362552.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACHDLD_000799"
     CDS             complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACHDLD_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN3362553.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACHDLD_000800"
     CDS             complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACHDLD_000800"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN3362554.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12856..13506)
                     /locus_tag="ACHDLD_000801"
     CDS             complement(12856..13506)
                     /locus_tag="ACHDLD_000801"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN3362555.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACHDLD_000802"
     CDS             complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACHDLD_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN3362556.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACHDLD_000803"
     CDS             complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACHDLD_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN3362557.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14949..15476)
                     /locus_tag="ACHDLD_000804"
     CDS             complement(14949..15476)
                     /locus_tag="ACHDLD_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN3362558.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15656..16441)
                     /locus_tag="ACHDLD_000805"
     CDS             complement(15656..16441)
                     /locus_tag="ACHDLD_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN3362559.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACHDLD_000806"
     CDS             complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACHDLD_000806"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN3362560.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17304..18683)
                     /locus_tag="ACHDLD_000807"
     CDS             complement(17304..18683)
                     /locus_tag="ACHDLD_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN3362561.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACHDLD_000808"
     CDS             complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACHDLD_000808"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN3362562.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACHDLD_000809"
     CDS             complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACHDLD_000809"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN3362563.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACHDLD_000810"
     CDS             complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACHDLD_000810"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN3362564.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACHDLD_000811"
     CDS             complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACHDLD_000811"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN3362565.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACHDLD_000812"
     CDS             complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACHDLD_000812"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN3362566.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22641..22865)
                     /locus_tag="ACHDLD_000813"
     CDS             complement(22641..22865)
                     /locus_tag="ACHDLD_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN3362567.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22918..23880)
                     /locus_tag="ACHDLD_000814"
     CDS             complement(22918..23880)
                     /locus_tag="ACHDLD_000814"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN3362568.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23890..24330)
                     /locus_tag="ACHDLD_000815"
     CDS             complement(23890..24330)
                     /locus_tag="ACHDLD_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3362569.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6671 a         5427 c         4250 g         8038 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1021 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1081 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1141 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1201 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1261 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1321 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1381 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1441 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1501 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1561 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1621 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1681 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1741 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1801 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1861 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1921 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     1981 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2041 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2101 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2161 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2221 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2281 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2341 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2401 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2461 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2521 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2581 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2641 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2701 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2761 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2821 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2881 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     2941 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3001 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3061 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3121 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3181 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3241 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3301 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3361 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3421 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3481 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3541 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3601 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3661 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3721 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3781 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3841 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3901 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     3961 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4021 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4081 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4141 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4201 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4261 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4321 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4381 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4441 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4501 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4561 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4621 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4681 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4741 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4801 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4861 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4921 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     4981 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5041 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5101 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5161 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5221 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5281 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5341 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5401 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5461 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5521 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5581 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5641 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5701 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5761 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5821 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5881 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     5941 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6001 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6061 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6121 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6181 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6241 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6301 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6361 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6421 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6481 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6541 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6601 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6661 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6721 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6781 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6841 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6901 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     6961 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7021 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7081 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7141 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7201 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7261 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7321 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7381 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7441 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7501 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7561 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7621 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7681 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7741 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7801 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7861 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7921 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     7981 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
     8041 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
     8101 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
     8161 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
     8221 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
     8281 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
     8341 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
     8401 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
     8461 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
     8521 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
     8581 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
     8641 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
     8701 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
     8761 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
     8821 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
     8881 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
     8941 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
     9001 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
     9061 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
     9121 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
     9181 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
     9241 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
     9301 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
     9361 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
     9421 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
     9481 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
     9541 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
     9601 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
     9661 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
     9721 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
     9781 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
     9841 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
     9901 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
     9961 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    10021 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    10081 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    10141 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    10201 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    10261 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    10321 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    10381 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    10441 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    10501 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    10561 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    10621 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    10681 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    10741 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    10801 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    10861 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    10921 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    10981 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    11041 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    11101 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    11161 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    11221 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    11281 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    11341 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    11401 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    11461 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    11521 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    11581 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    11641 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    11701 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    11761 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    11821 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    11881 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    11941 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    12001 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    12061 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    12121 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    12181 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    12241 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    12301 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    12361 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    12421 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    12481 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    12541 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    12601 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    12661 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    12721 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    12781 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    12841 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    12901 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    12961 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    13021 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    13081 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    13141 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    13201 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    13261 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    13321 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    13381 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    13441 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    13501 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    13561 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    13621 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    13681 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    13741 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    13801 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    13861 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    13921 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    13981 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    14041 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    14101 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    14161 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    14221 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    14281 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    14341 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    14401 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    14461 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    14521 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    14581 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    14641 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    14701 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    14761 caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa
    14821 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    14881 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    14941 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    15001 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    15061 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    15121 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    15181 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    15241 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    15301 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    15361 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    15421 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    15481 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    15541 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    15601 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    15661 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    15721 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    15781 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    15841 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    15901 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    15961 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    16021 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    16081 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    16141 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    16201 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    16261 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    16321 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    16381 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    16441 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    16501 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    16561 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    16621 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    16681 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    16741 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    16801 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    16861 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    16921 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    16981 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    17041 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    17101 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    17161 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    17221 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    17281 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    17341 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    17401 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    17461 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    17521 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    17581 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    17641 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    17701 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    17761 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    17821 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    17881 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    17941 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    18001 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    18061 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    18121 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    18181 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    18241 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    18301 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    18361 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    18421 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    18481 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    18541 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    18601 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    18661 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    18721 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    18781 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    18841 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    18901 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    18961 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    19021 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    19081 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    19141 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    19201 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    19261 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    19321 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    19381 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    19441 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    19501 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    19561 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    19621 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    19681 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    19741 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    19801 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    19861 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    19921 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    19981 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    20041 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    20101 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    20161 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    20221 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    20281 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    20341 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    20401 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    20461 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    20521 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    20581 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    20641 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    20701 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    20761 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    20821 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    20881 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    20941 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    21001 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    21061 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    21121 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    21181 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    21241 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    21301 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    21361 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    21421 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    21481 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    21541 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    21601 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    21661 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    21721 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    21781 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    21841 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    21901 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    21961 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    22021 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    22081 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    22141 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    22201 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    22261 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    22321 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    22381 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    22441 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    22501 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    22561 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    22621 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    22681 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    22741 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    22801 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    22861 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    22921 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    22981 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    23041 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    23101 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    23161 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    23221 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    23281 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    23341 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    23401 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    23461 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    23521 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    23581 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    23641 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    23701 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    23761 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    23821 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    23881 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    23941 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    24001 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    24061 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    24121 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    24181 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    24241 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    24301 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    24361 caaatctttt gatgttcaaa ctatat
//
LOCUS       DAZAOG010000022        23885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000022 DAZAOG010000000
VERSION     DAZAOG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.022"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHDLD_000816"
     CDS             complement(55..789)
                     /locus_tag="ACHDLD_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN3362570.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHDLD_000817"
     CDS             1116..1526
                     /locus_tag="ACHDLD_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN3362571.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHDLD_000818"
     CDS             1519..2220
                     /locus_tag="ACHDLD_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN3362572.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHDLD_000819"
     CDS             2220..3806
                     /locus_tag="ACHDLD_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362573.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHDLD_000820"
     CDS             3825..4046
                     /locus_tag="ACHDLD_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN3362574.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHDLD_000821"
     CDS             4043..4804
                     /locus_tag="ACHDLD_000821"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN3362575.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHDLD_000822"
     CDS             5184..5693
                     /locus_tag="ACHDLD_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN3362576.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHDLD_000823"
     CDS             complement(5768..6307)
                     /locus_tag="ACHDLD_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN3362577.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHDLD_000824"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHDLD_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN3362578.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHDLD_000825"
     CDS             7320..8171
                     /locus_tag="ACHDLD_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN3362579.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHDLD_000826"
     CDS             8199..8834
                     /locus_tag="ACHDLD_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN3362580.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHDLD_000827"
     CDS             8854..9627
                     /locus_tag="ACHDLD_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3362581.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHDLD_000828"
     CDS             10035..10832
                     /locus_tag="ACHDLD_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN3362582.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHDLD_000829"
     CDS             10810..11223
                     /locus_tag="ACHDLD_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3362583.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHDLD_000830"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHDLD_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN3362584.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHDLD_000831"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHDLD_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN3362585.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHDLD_000832"
     CDS             16000..16560
                     /locus_tag="ACHDLD_000832"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN3362586.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHDLD_000833"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHDLD_000833"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN3362587.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHDLD_000834"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHDLD_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN3362588.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHDLD_000835"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHDLD_000835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN3362589.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHDLD_000836"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHDLD_000836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN3362590.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHDLD_000837"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHDLD_000837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN3362591.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHDLD_000838"
     CDS             21907..22485
                     /locus_tag="ACHDLD_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3362592.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHDLD_000839"
     CDS             22537..23019
                     /locus_tag="ACHDLD_000839"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN3362593.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHDLD_000840"
     CDS             23136..23510
                     /locus_tag="ACHDLD_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362594.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7810 a         4289 c         5123 g         6663 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtc
//
LOCUS       DAZAOG010000023        23690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000023 DAZAOG010000000
VERSION     DAZAOG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.023"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1394
                     /locus_tag="ACHDLD_000841"
     CDS             54..1394
                     /locus_tag="ACHDLD_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3362595.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1612..2055
                     /locus_tag="ACHDLD_000842"
     CDS             1612..2055
                     /locus_tag="ACHDLD_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN3362596.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2559..3977)
                     /gene="nhaC"
                     /locus_tag="ACHDLD_000843"
     CDS             complement(2559..3977)
                     /gene="nhaC"
                     /locus_tag="ACHDLD_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN3362597.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4380..5603)
                     /locus_tag="ACHDLD_000844"
     CDS             complement(4380..5603)
                     /locus_tag="ACHDLD_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3362598.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5634..6473)
                     /locus_tag="ACHDLD_000845"
     CDS             complement(5634..6473)
                     /locus_tag="ACHDLD_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3362599.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6490..7170)
                     /locus_tag="ACHDLD_000846"
     CDS             complement(6490..7170)
                     /locus_tag="ACHDLD_000846"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN3362600.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7172..8245)
                     /locus_tag="ACHDLD_000847"
     CDS             complement(7172..8245)
                     /locus_tag="ACHDLD_000847"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN3362601.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQESPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8301..8539)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8582..8815)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8892..9563)
                     /locus_tag="ACHDLD_000848"
     CDS             complement(8892..9563)
                     /locus_tag="ACHDLD_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3362602.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9650..12298)
                     /gene="mgtA"
                     /locus_tag="ACHDLD_000849"
     CDS             complement(9650..12298)
                     /gene="mgtA"
                     /locus_tag="ACHDLD_000849"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN3362603.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12640..12814)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12987..13424
                     /gene="ndk"
                     /locus_tag="ACHDLD_000850"
     CDS             12987..13424
                     /gene="ndk"
                     /locus_tag="ACHDLD_000850"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN3362604.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13446..14312)
                     /locus_tag="ACHDLD_000851"
     CDS             complement(13446..14312)
                     /locus_tag="ACHDLD_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN3362605.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14472..16565)
                     /locus_tag="ACHDLD_000852"
     CDS             complement(14472..16565)
                     /locus_tag="ACHDLD_000852"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3362606.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17314..17937)
                     /locus_tag="ACHDLD_000853"
     CDS             complement(17314..17937)
                     /locus_tag="ACHDLD_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362607.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18096..19493)
                     /locus_tag="ACHDLD_000854"
     CDS             complement(18096..19493)
                     /locus_tag="ACHDLD_000854"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3362608.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19525..20496)
                     /locus_tag="ACHDLD_000855"
     CDS             complement(19525..20496)
                     /locus_tag="ACHDLD_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362609.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20870..21295
                     /locus_tag="ACHDLD_000856"
     CDS             20870..21295
                     /locus_tag="ACHDLD_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362610.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21783..22484)
                     /locus_tag="ACHDLD_000857"
     CDS             complement(21783..22484)
                     /locus_tag="ACHDLD_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3362611.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22500..22940)
                     /locus_tag="ACHDLD_000858"
     CDS             complement(22500..22940)
                     /locus_tag="ACHDLD_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN3362612.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22940..23515)
                     /locus_tag="ACHDLD_000859"
     CDS             complement(22940..23515)
                     /locus_tag="ACHDLD_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN3362613.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6828 a         4906 c         4071 g         7885 t
ORIGIN      
        1 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
       61 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
      121 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
      181 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
      241 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
      301 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
      361 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
      421 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
      481 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
      541 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
      601 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
      661 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
      721 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
      781 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
      841 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
      901 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
      961 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
     1021 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
     1081 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
     1141 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
     1201 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
     1261 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
     1321 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
     1381 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
     1441 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
     1501 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
     1561 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
     1621 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
     1681 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
     1741 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
     1801 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
     1861 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
     1921 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
     1981 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
     2041 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
     2101 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
     2161 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
     2221 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
     2281 atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc
     2341 cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga
     2401 aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg
     2461 tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga
     2521 ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc
     2581 ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca
     2641 ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt
     2701 gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag
     2761 ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct
     2821 ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca
     2881 ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc
     2941 gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat
     3001 ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct
     3061 ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg
     3121 atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact
     3181 gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt
     3241 aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca
     3301 tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg
     3361 aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt
     3421 ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac
     3481 atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag
     3541 cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca
     3601 ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg
     3661 acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat
     3721 acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg
     3781 ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac
     3841 atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg
     3901 ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt
     3961 gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt
     4021 taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata aaaataaaaa
     4081 gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct
     4141 gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt cgtcaattag
     4201 gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc gctactgcgt
     4261 gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa
     4321 tattttttca tttttctgaa aattattttt ataactttca cacacaagtt tattttttac
     4381 tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc ttgcttgatt
     4441 ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag tagttctgct
     4501 gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa ttcttctgtg
     4561 tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg tatgacttgg
     4621 ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc tatttcttca
     4681 tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga aggagtaacg
     4741 atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc
     4801 gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt ttgccgattt
     4861 cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc agctactcct
     4921 ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg tagagcgttg
     4981 attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc aggtgctgct
     5041 gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc taaagattct
     5101 gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac atcctcaaaa
     5161 aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc ttcccctggg
     5221 gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac tatgctggcg
     5281 gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat acctggtaaa
     5341 gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat ataacgtgca
     5401 tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc tttttctaat
     5461 tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg atgttcgtgg
     5521 atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc cttcaattgg
     5581 tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt ttcttatttt
     5641 ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc gattactttg
     5701 actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt gttttcatct
     5761 tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac tggttctaag
     5821 aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct attgatcact
     5881 gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc agaaatgtct
     5941 agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt tgccttatcc
     6001 actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt tgtaacatca
     6061 ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa agtgttagaa
     6121 taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt tccgtgttct
     6181 ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat cgatccatct
     6241 gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa attaatgttc
     6301 tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc aataatccct
     6361 agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct acaccctgcc
     6421 aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt cattttttct
     6481 cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt gacttaaaaa
     6541 aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga atcgattata
     6601 ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag ccatggtagt
     6661 cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag gcaaaccttc
     6721 taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt ctgccgcttc
     6781 gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac
     6841 aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc caactaagaa
     6901 acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt
     6961 gatcagctta tcaagtattt caaatagcag acggttagct agtaatccat tagggcgtgt
     7021 cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa
     7081 agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca aataaacatt
     7141 gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc actttgatac
     7201 gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg atcaaataat
     7261 ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa ctccctaacg
     7321 gcgattcttg taataactct acattgccat ataaaatatt cgctgttact tgatatttgc
     7381 tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt aattccacca
     7441 gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt
     7501 ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca ggattgctga
     7561 agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc gctaccttgt
     7621 tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt aaacctaact
     7681 ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga tcaagtgcac
     7741 tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt gcgattgcta
     7801 ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt tcttccaaac
     7861 cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat tttccagaat
     7921 actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct
     7981 gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag agctgactca
     8041 gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa ttcatcacac
     8101 gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca tcgccttttt
     8161 caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata
     8221 cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa
     8281 gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc tcatgttcaa
     8341 gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga tatcggtcgc
     8401 agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag
     8461 tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt
     8521 accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata aactatgtca
     8581 gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca
     8641 acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta
     8701 cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc
     8761 agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt
     8821 tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat
     8881 tttttctata tttatttttc tgttttttta tttaacattt caataacagt tcgaagatct
     8941 tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa
     9001 ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact
     9061 aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc
     9121 agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc
     9181 aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc
     9241 gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat
     9301 ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct
     9361 cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt
     9421 ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc
     9481 tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct
     9541 aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt
     9601 tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt tataagagtt
     9661 cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga taagcgatga
     9721 ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac
     9781 caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca
     9841 tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca
     9901 ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa
     9961 tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca ggacaaataa
    10021 caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga ccaaagtaaa
    10081 ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat
    10141 ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc aaaaacaaaa
    10201 gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc acggaaaaca
    10261 tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt
    10321 ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt
    10381 ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt
    10441 tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac
    10501 gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt
    10561 cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca
    10621 attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc
    10681 cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg
    10741 tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct gactcatctt
    10801 tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta
    10861 agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta
    10921 atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg
    10981 cgccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac
    11041 gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta
    11101 aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat
    11161 agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca
    11221 tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtacataaca
    11281 catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt ccttttttgg
    11341 ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg
    11401 ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa
    11461 gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg
    11521 atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg
    11581 ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac
    11641 tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt
    11701 cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg
    11761 aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg
    11821 aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat
    11881 tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg
    11941 aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg aagataatgg
    12001 ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa
    12061 taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta
    12121 caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac
    12181 gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca aataattctt
    12241 gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct
    12301 caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc
    12361 atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat agccctggcc
    12421 tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact
    12481 aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc caattagacc
    12541 acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat
    12601 actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt
    12661 tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt
    12721 atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat
    12781 gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac
    12841 taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac
    12901 gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga
    12961 aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg
    13021 gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag ggactggcca
    13081 ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact
    13141 tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt
    13201 atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggagcaacaa
    13261 aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa
    13321 acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc
    13381 cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg
    13441 ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga
    13501 ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt cgtcttttct
    13561 tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc
    13621 actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc
    13681 tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga
    13741 tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa
    13801 ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca
    13861 aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt
    13921 aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag attcaaaacc
    13981 aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa
    14041 ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt
    14101 ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct
    14161 aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact
    14221 agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt cttgttgact
    14281 cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt
    14341 tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg
    14401 tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt
    14461 ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt tactggatat
    14521 aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg ttcgcctttt
    14581 tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt tttcggatca
    14641 atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct
    14701 gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc atcgattcct
    14761 gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg
    14821 gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc atgctgagaa
    14881 gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt
    14941 aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc tttagtataa
    15001 ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat
    15061 gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt cgagatccca
    15121 aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac
    15181 gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact
    15241 gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc
    15301 gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat
    15361 tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat
    15421 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
    15481 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
    15541 ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag
    15601 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
    15661 tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta
    15721 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
    15781 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
    15841 ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca
    15901 tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg
    15961 attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg
    16021 ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg
    16081 atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa
    16141 accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc
    16201 aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg
    16261 actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta
    16321 tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa
    16381 gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg
    16441 atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag
    16501 aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt
    16561 gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa
    16621 atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc
    16681 acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt
    16741 cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa
    16801 aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt
    16861 ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    16921 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    16981 aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt
    17041 caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa
    17101 taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca
    17161 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccttcggt
    17221 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    17281 aggtcaaaac gctgatttca caacctctgt ttttcattgt ccataggatt gatattgtcc
    17341 ttgtgatgac tcattcgtaa taattgaggc ttgattttct tcacttcccg ttatcgtttg
    17401 tccactgcca gaatctgaag gacttgttcc tgtcgactcg tttttttgag aagagacagt
    17461 tgttttcttg acttgttcat ttgtcttttt ccattccgct aaacgatcgg cgaccacttt
    17521 tttgtctact ttttcagaat aagcggtgat cacttttcca tttttcaaac gggctacttt
    17581 gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg tctttaaatt gatattgtct
    17641 gagagaaatc gcactaagta cgtcatcgat attaataaat tgattttctt cttttccttt
    17701 tggcaaataa atgtccaatt cgcctttttc gtaattggca gttattttat actccgccca
    17761 ccaataagtt gagaattctt ttttgaaatt atctactgat tctttgcttg ctacgatttt
    17821 ttctgtttct ttctgagcaa gatctgctgt catttttcct gaaaatgctg cggataaaat
    17881 aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt tcctcatgct
    17941 ttcctccact cctatttaat tttgactatt ctattatata caaaaaatga aatagaaaga
    18001 caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg
    18061 gagaatttca cgcatttgct ctcaactatt ctttcttaag cctcaaatcc gtttttatct
    18121 gatacgtctt tgaaccaatg acccgatttt ttgattgttc tttttccatc ttgtgctaag
    18181 tctactgaaa taaagccata tcgattctta tatgcattgg tccacgacca gttatccata
    18241 catgtccaca tatgatagcc taaacaattg gcaccttctt gaatggcttg atggacatat
    18301 ttcagatgag attggacaaa ttcgatccga taatcatcgt ggatcatgcc atcttctttg
    18361 ataaatcgtg cttcgttttc tacacccata ccgttttcag aaataaagca gtcgatattt
    18421 ccataatgat ctcgtacatt gatcaaaata tcatagattc cttgttcata gatctcccat
    18481 ccgcgataag gattcatttt acgaccaggc atttcataat tgtcgaaata atcctcaggc
    18541 attggaccgt ttttagtatc gattggtgtt tcttttgctt ttacccggcg tggctggtaa
    18601 taattgatac ctaaaagatc tactgtattt tctttgataa ccgtcaaatc ctctgcatgg
    18661 atcacaggca tgagatcaag ttcttttact atctcaatca attcacttgg aaactcgcct
    18721 tttacagctg gatcaaggaa tgaacgattg aagaacgcat ctgctatttg cgctgcttta
    18781 acatcttcag ggtttgattc gtctcttgga tagcttggtg tcagattcaa gatgatacca
    18841 ataacaccac cttgctccat ttcatggtaa gatttgatgg ctaacgctga agccaacgct
    18901 tcatgatagc caacttggat cgcttttttc atatctgact cttctggata atgccatcga
    18961 tataaataac ctgcttcgac tggaacgatt ggttcattat gagtaaacca tttttttact
    19021 ttcgaaccaa acaattcaaa acaacttttg gcatattttt cataggcttc aaccgtttct
    19081 cggttcaacc atccgccttt ttcttgcaga tacatcggca tatcgaaatg atacagattc
    19141 acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg
    19201 gcttcttcgt tgattgttcc agattcatca ggtatcaaac ggctccattg gatagatgtt
    19261 cgaaatgaat tatgcccagt ctgtttcatc aactgtacat cctcttgata cttcgtataa
    19321 acttgcgagg ttttttcagg tcctacttga tcaaagaact tttgcggttc ttttttgtac
    19381 caataatcaa aaatgttctc tccttttcca tcgcctgaaa tccttccttc tgtttgtggg
    19441 ccacttgctg cagaacccca ccaaaaattt gctggaaatt gatatttttt catcaaagca
    19501 cctctttttc ttttttagtt cgttttagtt gattccatgt atgatatttt ccatcacaat
    19561 cactttttca ttttcttcct cttctttttt gattcgtttt aacagctgtt ctgctgctcg
    19621 ttccccgatt ttatctgttg gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt
    19681 tttatctaaa taatcatagt gactgaacgc aatatccttc ccgattctga caccttcttg
    19741 attgaaacat tcgatggcac caatcgacat attgtaattg ctgacaaaaa tagcagttgc
    19801 ccctttttct aataatgctt ttgccccgta atagccgctt tttctagaat agtctcctgt
    19861 aaagacctct aattggatcg atggatcatt ttggaccgct tctttggctc ctgctaaacg
    19921 ttctcttgct acgtagtctg tttgagtggc agcaataaat ccgatttttt tatgtccttg
    19981 ttcaccatga tgagcgatca cttttgctac actttctcga tcattcacta atattgtatc
    20041 aatacctgct ggtccttctg gacaattcaa tgcgataact ggaatactca tttttgtgag
    20101 catttcaaat ccagaccact cttcttcgga taagaagaca atcagcccat cgatcacatg
    20161 cttcatcagg tattcaattt tttgttcaat tttttctggc tgatcggcaa acccgcttaa
    20221 taataagcta tacccatttt cttcagctac tctttcgatc cctgagacaa cttccgaccc
    20281 aaaacggctg gaaatcccgt ttatcaaaag tccaatagag aagcttcgat tattcttcaa
    20341 ccctttagca atgatgtttt ctttatagtc caacgctgaa atcgcttctg ctattttttt
    20401 catgttcttt tccttcagtt gctgaccatt caagtaacgg gaaactgttc ccactgaaac
    20461 accagccatc ttagccacat cacggatcga tgccatacat tctcactcct gccagttaca
    20521 tttgatttct ctttcttgtt gttcgattat gatgaccgca ttctttgcat agtgttgctt
    20581 gtcatcaata tccatataac taaagtcttc agtagagcaa cttcctctca ctattaatta
    20641 tacttaaaca agtatttgaa ccggttcatt tttaatatag cattttttta aaagattgca
    20701 acactttttt aaaacgcttt ctgaaagtgg cggatttttt gcctttttaa aattttattc
    20761 taaaatttac tctatttgtg ggtgttttgt gcgtttttct cttccatccg tttgaagttt
    20821 tacattccaa tggtataatt tctttatcct atcaaatggg ggaagtattt tgaagaaatt
    20881 aatcgtacta tttattttgt ctgcgggact tttgacattg actggctgtg atttgtttgg
    20941 cggctctgac caatcctcgg acgaaacaac cgattcttct gtcttaaaag cggaaaaagc
    21001 agataccacc aacagtgtat atacgctaaa agtacgtaaa gatatggatt tatccgcggg
    21061 acagttgaca tttacagata aaaaaatgga atggacacgc acgtatcaga cacaaggaac
    21121 tgattcttct gaatcatctg aagaaaaaac agtgctgaca gatatcaaga tcactaccaa
    21181 agatgacgaa tatattattt caggaaaaga aaataaaaaa gaaacaacag tcatatttac
    21241 aaaaatcggt aattaccgtt tgaaggatga ggatggcaac atctacagtc tatagtgccg
    21301 tctgttggat cttcttttgt ttctcccctt cttttctgct atagtaaaga agcaagatga
    21361 ttgtaaagga gacgaaatgg atgatcgata gtcctgatta ttgtacatgt aataacaaca
    21421 aaaatatcca tacagaattt aatgaccaag gaagcttgag catatgcgac aactgtcaca
    21481 aactagtcaa agattcattt aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa
    21541 gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa
    21601 actcagcttc ttcctttgat caagcttcat cagtaaaaca aacagtggat catttcagtt
    21661 tacaaatcaa agagtaaaga tttatttatt tcaaatattt gaatataaaa taaccaaacg
    21721 atagaaaggc ttattctaca tcacgtagta atttttcttt cccgtcgttt ggttttttat
    21781 ttttagccta attcttgacc ttttgcatgc tggttatccg taaaatattt tccagcaggc
    21841 tttccttcca attcagctaa tttttcttgg atctgacgga tcaccttttt tgcatcttct
    21901 ggattttcta caaaatcgaa tttatcccca tccacttgta ttttaggaca tacatcaaaa
    21961 tcttcatacc attgattata gcgggtgttc aacgtttcat aataggtata taacgtctca
    22021 tcgctttcta gctgttcata gtcacgccca cgttttttga tacgttctag catcgtgtcc
    22081 aaagaaactt tgatatggac catcaaatca ggccgttttt ttggagcaac atcatctaac
    22141 tcgtttagca tcgtatctaa taagctatcg tactgctgta cttcgatatc cgttacccga
    22201 cctaaatcag cgtttagatg gaacaacagg ctatcttcat agatggatcg gtctagcaca
    22261 ttgtcatcgt tcactaaagc atttttcatt gctaaaaatc gcttgttcaa aaagaagatt
    22321 tgcaataaaa aagcgtactg ctcaggattt gaataaaaga gcggcaatac ttcgttatct
    22381 tcgacatttt cataaaatgg tttagaatca atctcttttg caagcatatc cgtcaaactg
    22441 ctcttacctg caccaatcgt tcctgaaatc aaaatcactg acatacaacc cttcctcttc
    22501 tataagtact cgcctacgta agtttcaaca ggcaatgttt caaaatcata ttcacgtaca
    22561 gcttggtctg attttaaata cgtatggaga ctttgtgtaa tcatcaagtt cgtaggtacc
    22621 gtctcttctt gtacaatgat catcggtttt ttcaacatag ttgccacacc taattcgtaa
    22681 gccgttcctg gatcgattgt ttgatgctca aagtcaagga tagcaacaac aaattctgca
    22741 tttgtcagtt cttccatatc tttttcgtat acttcctgcg cccatccagc gctgaatagt
    22801 tcataattgc tttcttggtg atgacgtgga gagtagaaac ttgtgactgt tttgttttcc
    22861 tccaacgctt tttcgatacg acttactcga tcgatttgct cctctgaaaa aaatggtcct
    22921 gctaaatata tttgactcat tattttgcct ccttcaataa taactgattt ttaatagata
    22981 ctgctcgtaa ttcattgacc agttccaatg gattgttgga aaagattact tgatccaacc
    23041 attttttatc cataaaatcc tcttttgcaa aagtttctaa taattgttgc aagccgctgt
    23101 aataaccgcc aatattcaat attccgattg gcttgtcggg cgtcagaccg attgctatcc
    23161 aagaaagaag ttgtgagaat tcctccaatg tacctagacc tccagggaaa acgaggacag
    23221 catctccctt ttcaaataat aatacctttc tttcatcgat cgtttctgta ggaataaatg
    23281 tagtaacatc ttttccaggc aattcagttt caaaaagccc attcggatag atacctgtga
    23341 cttttccgtt attttccagt actgttttac taatcactcc cataatcccg gagtatgaac
    23401 caccgtaaac cagttcgatt tcttcttgtg ccatgtattt tcctaacgtt tgcgcaataa
    23461 atttgtaact ttctttatta ccaaaacggg aaccgcaaaa aacagtaagt ttcataaaaa
    23521 aacactccct aactcaaaag tctatcttca ttctacacgt gtttttttaa aataaaagac
    23581 tagattttgt agttttttag tggtatatag gatagacgcc actatatata gtatttagaa
    23641 aaaaatcatt tttcagccat tttatagata aaacaaaaat aaataacgtg
//
LOCUS       DAZAOG010000024        22969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000024 DAZAOG010000000
VERSION     DAZAOG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.024"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(330..2711)
                     /locus_tag="ACHDLD_000860"
     CDS             complement(330..2711)
                     /locus_tag="ACHDLD_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN3362614.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2701..3348)
                     /gene="recU"
                     /locus_tag="ACHDLD_000861"
     CDS             complement(2701..3348)
                     /gene="recU"
                     /locus_tag="ACHDLD_000861"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN3362615.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3412..3951
                     /locus_tag="ACHDLD_000862"
     CDS             3412..3951
                     /locus_tag="ACHDLD_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN3362616.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4041..4460
                     /gene="gpsB"
                     /locus_tag="ACHDLD_000863"
     CDS             4041..4460
                     /gene="gpsB"
                     /locus_tag="ACHDLD_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN3362617.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4482..4849
                     /gene="rnpB"
                     /locus_tag="ACHDLD_000864"
     ncRNA           4482..4849
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHDLD_000864"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5058..6224
                     /locus_tag="ACHDLD_000865"
     CDS             5058..6224
                     /locus_tag="ACHDLD_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN3362618.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6270..7766
                     /locus_tag="ACHDLD_000866"
     CDS             6270..7766
                     /locus_tag="ACHDLD_000866"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN3362619.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7847..8530
                     /locus_tag="ACHDLD_000867"
     CDS             7847..8530
                     /locus_tag="ACHDLD_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3362620.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9175..10365
                     /locus_tag="ACHDLD_000868"
     CDS             9175..10365
                     /locus_tag="ACHDLD_000868"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN3362621.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10467..11420
                     /locus_tag="ACHDLD_000869"
     CDS             10467..11420
                     /locus_tag="ACHDLD_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN3362622.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11489..12838
                     /locus_tag="ACHDLD_000870"
     CDS             11489..12838
                     /locus_tag="ACHDLD_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3362623.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13021..13344)
                     /locus_tag="ACHDLD_000871"
     CDS             complement(13021..13344)
                     /locus_tag="ACHDLD_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362624.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13473..14636
                     /locus_tag="ACHDLD_000872"
     CDS             13473..14636
                     /locus_tag="ACHDLD_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3362625.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14706..16055
                     /locus_tag="ACHDLD_000873"
     CDS             14706..16055
                     /locus_tag="ACHDLD_000873"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3362626.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16100..16531)
                     /locus_tag="ACHDLD_000874"
     CDS             complement(16100..16531)
                     /locus_tag="ACHDLD_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3362627.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16919..19561
                     /gene="alaS"
                     /locus_tag="ACHDLD_000875"
     CDS             16919..19561
                     /gene="alaS"
                     /locus_tag="ACHDLD_000875"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN3362628.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19761..20357
                     /locus_tag="ACHDLD_000876"
     CDS             19761..20357
                     /locus_tag="ACHDLD_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3362629.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20479..21627
                     /locus_tag="ACHDLD_000877"
     CDS             20479..21627
                     /locus_tag="ACHDLD_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3362630.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21665..22078)
                     /locus_tag="ACHDLD_000878"
     CDS             complement(21665..22078)
                     /locus_tag="ACHDLD_000878"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN3362631.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22157..22588
                     /locus_tag="ACHDLD_000879"
     CDS             22157..22588
                     /locus_tag="ACHDLD_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN3362632.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22687..22920)
                     /locus_tag="ACHDLD_000880"
     CDS             complement(22687..22920)
                     /locus_tag="ACHDLD_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3362633.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         7396 a         4038 c         4801 g         6734 t
ORIGIN      
        1 gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac
       61 aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat
      121 tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt
      181 tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta
      241 atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa
      301 ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg
      361 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
      421 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
      481 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
      541 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
      601 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
      661 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
      721 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
      781 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
      841 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
      901 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
      961 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     1021 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     1081 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     1141 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     1201 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
     1261 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
     1321 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
     1381 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
     1441 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
     1501 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
     1561 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
     1621 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
     1681 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
     1741 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
     1801 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
     1861 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
     1921 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
     1981 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
     2041 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
     2101 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
     2161 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
     2221 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
     2281 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
     2341 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
     2401 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
     2461 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
     2521 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
     2581 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
     2641 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
     2701 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
     2761 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
     2821 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
     2881 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
     2941 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
     3001 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
     3061 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
     3121 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
     3181 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
     3241 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
     3301 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
     3361 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
     3421 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
     3481 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
     3541 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
     3601 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
     3661 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
     3721 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
     3781 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
     3841 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
     3901 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
     3961 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
     4021 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
     4081 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
     4141 atatcatcaa agattatgaa gcttacagca aagaactcct tgctttacaa gaagaaaacg
     4201 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
     4261 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
     4321 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
     4381 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
     4441 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
     4501 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
     4561 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
     4621 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
     4681 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
     4741 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
     4801 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
     4861 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
     4921 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
     4981 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
     5041 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
     5101 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
     5161 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
     5221 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
     5281 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
     5341 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
     5401 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
     5461 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
     5521 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
     5581 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
     5641 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
     5701 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
     5761 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
     5821 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
     5881 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
     5941 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
     6001 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
     6061 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
     6121 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
     6181 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
     6241 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
     6301 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
     6361 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
     6421 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
     6481 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
     6541 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
     6601 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
     6661 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
     6721 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
     6781 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
     6841 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
     6901 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
     6961 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
     7021 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
     7081 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
     7141 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
     7201 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
     7261 aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
     7321 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
     7381 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
     7441 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
     7501 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
     7561 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
     7621 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
     7681 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
     7741 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
     7801 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
     7861 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
     7921 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
     7981 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
     8041 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
     8101 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
     8161 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
     8221 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
     8281 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
     8341 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
     8401 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
     8461 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
     8521 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
     8581 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
     8641 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
     8701 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
     8761 tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa
     8821 aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa
     8881 taaataaatt aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt
     8941 gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc
     9001 atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa
     9061 ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga
     9121 cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct
     9181 tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta
     9241 tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa
     9301 aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa
     9361 aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc
     9421 aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca
     9481 gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta
     9541 atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt
     9601 tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag
     9661 aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat
     9721 ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa
     9781 aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt
     9841 gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta
     9901 tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg
     9961 ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca
    10021 gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg
    10081 gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct
    10141 gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag
    10201 aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg
    10261 acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca
    10321 gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt
    10381 tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt
    10441 agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac
    10501 aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg
    10561 cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg
    10621 taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc
    10681 atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga
    10741 acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta
    10801 cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata
    10861 ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg
    10921 attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt
    10981 cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag
    11041 caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt
    11101 atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg
    11161 ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat
    11221 ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt
    11281 caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct
    11341 tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa
    11401 aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga
    11461 ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga
    11521 agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt
    11581 tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac
    11641 aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc
    11701 aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata
    11761 tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat
    11821 ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct
    11881 atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct
    11941 gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg
    12001 caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga
    12061 cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc
    12121 gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg
    12181 agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat
    12241 catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt
    12301 cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg
    12361 aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct
    12421 tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct
    12481 ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt
    12541 catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt
    12601 tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga
    12661 acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat
    12721 cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat
    12781 ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct
    12841 aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat
    12901 gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt
    12961 ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt
    13021 ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac
    13081 caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt
    13141 agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag
    13201 attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt
    13261 agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc
    13321 aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt actttacttt
    13381 gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat
    13441 aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt
    13501 ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg
    13561 aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt
    13621 tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg
    13681 cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag
    13741 acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg
    13801 tatttttgga accagctttt aatgttttat tactcatacc atttggcatt tatttaagat
    13861 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
    13921 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
    13981 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
    14041 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
    14101 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
    14161 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
    14221 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
    14281 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
    14341 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
    14401 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
    14461 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
    14521 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
    14581 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
    14641 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
    14701 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
    14761 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
    14821 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
    14881 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
    14941 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
    15001 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
    15061 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
    15121 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
    15181 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
    15241 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
    15301 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
    15361 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
    15421 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
    15481 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
    15541 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
    15601 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
    15661 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
    15721 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
    15781 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
    15841 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
    15901 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
    15961 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
    16021 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
    16081 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
    16141 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
    16201 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
    16261 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
    16321 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
    16381 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
    16441 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
    16501 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
    16561 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
    16621 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga
    16681 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
    16741 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
    16801 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
    16861 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
    16921 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg
    16981 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
    17041 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
    17101 aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
    17161 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
    17221 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
    17281 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
    17341 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
    17401 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
    17461 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
    17521 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
    17581 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
    17641 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
    17701 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
    17761 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
    17821 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
    17881 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
    17941 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
    18001 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
    18061 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
    18121 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
    18181 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
    18241 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
    18301 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
    18361 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta
    18421 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
    18481 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
    18541 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
    18601 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
    18661 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
    18721 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
    18781 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
    18841 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
    18901 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
    18961 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
    19021 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
    19081 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
    19141 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
    19201 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
    19261 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
    19321 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
    19381 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
    19441 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
    19501 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
    19561 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
    19621 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
    19681 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
    19741 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
    19801 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
    19861 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
    19921 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
    19981 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
    20041 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
    20101 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
    20161 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
    20221 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
    20281 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
    20341 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
    20401 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
    20461 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
    20521 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
    20581 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
    20641 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
    20701 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
    20761 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
    20821 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
    20881 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
    20941 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
    21001 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
    21061 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
    21121 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
    21181 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
    21241 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
    21301 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
    21361 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
    21421 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
    21481 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
    21541 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
    21601 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
    21661 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
    21721 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
    21781 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
    21841 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
    21901 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
    21961 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
    22021 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
    22081 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
    22141 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
    22201 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
    22261 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
    22321 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
    22381 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
    22441 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
    22501 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
    22561 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
    22621 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
    22681 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
    22741 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
    22801 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
    22861 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
    22921 agcactcacg acctttctaa agtctgatca tagccttcat tataaagag
//
LOCUS       DAZAOG010000025        22462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000025 DAZAOG010000000
VERSION     DAZAOG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.025"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHDLD_000881"
     CDS             <1..395
                     /locus_tag="ACHDLD_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN3362634.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHDLD_000882"
     CDS             398..1147
                     /locus_tag="ACHDLD_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362635.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHDLD_000883"
     CDS             1159..1428
                     /locus_tag="ACHDLD_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362636.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHDLD_000884"
     CDS             1433..1597
                     /locus_tag="ACHDLD_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362637.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHDLD_000885"
     CDS             1590..1892
                     /locus_tag="ACHDLD_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362638.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHDLD_000886"
     CDS             1889..2050
                     /locus_tag="ACHDLD_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3362639.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHDLD_000887"
     CDS             2047..2352
                     /locus_tag="ACHDLD_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362640.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHDLD_000888"
     CDS             2352..2708
                     /locus_tag="ACHDLD_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFN3362641.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHDLD_000889"
     CDS             2668..2913
                     /locus_tag="ACHDLD_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362642.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHDLD_000890"
     CDS             2910..3329
                     /locus_tag="ACHDLD_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN3362643.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHDLD_000891"
     CDS             3326..3883
                     /locus_tag="ACHDLD_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFN3362644.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHDLD_000892"
     CDS             3880..4176
                     /locus_tag="ACHDLD_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362645.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHDLD_000893"
     CDS             4253..4666
                     /locus_tag="ACHDLD_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN3362646.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACHDLD_000894"
     CDS             5012..5386
                     /locus_tag="ACHDLD_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362647.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACHDLD_000895"
     CDS             5423..5590
                     /locus_tag="ACHDLD_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362648.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACHDLD_000896"
     CDS             5616..5960
                     /locus_tag="ACHDLD_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN3362649.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACHDLD_000897"
     CDS             5965..6246
                     /locus_tag="ACHDLD_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362650.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACHDLD_000898"
     CDS             6349..6663
                     /locus_tag="ACHDLD_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN3362651.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACHDLD_000899"
     CDS             6641..8335
                     /locus_tag="ACHDLD_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3362652.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACHDLD_000900"
     CDS             8355..9533
                     /locus_tag="ACHDLD_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3362653.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACHDLD_000901"
     CDS             9496..10182
                     /locus_tag="ACHDLD_000901"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN3362654.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACHDLD_000902"
     CDS             10182..11342
                     /locus_tag="ACHDLD_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3362655.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACHDLD_000903"
     CDS             11352..12227
                     /locus_tag="ACHDLD_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362656.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACHDLD_000904"
     CDS             12224..12535
                     /locus_tag="ACHDLD_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362657.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACHDLD_000905"
     CDS             12525..12878
                     /locus_tag="ACHDLD_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3362658.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACHDLD_000906"
     CDS             12868..13269
                     /locus_tag="ACHDLD_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362659.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACHDLD_000907"
     CDS             13262..13666
                     /locus_tag="ACHDLD_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362660.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACHDLD_000908"
     CDS             13678..14286
                     /locus_tag="ACHDLD_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3362661.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACHDLD_000909"
     CDS             14306..14668
                     /locus_tag="ACHDLD_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362662.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACHDLD_000910"
     CDS             14671..14853
                     /locus_tag="ACHDLD_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362663.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACHDLD_000911"
     CDS             14870..18289
                     /locus_tag="ACHDLD_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN3362664.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACHDLD_000912"
     CDS             18340..19077
                     /locus_tag="ACHDLD_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3362665.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACHDLD_000913"
     CDS             19087..21378
                     /locus_tag="ACHDLD_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3362666.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>22462
                     /locus_tag="ACHDLD_000914"
     CDS             21402..>22462
                     /locus_tag="ACHDLD_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN3362667.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGST"
BASE COUNT         8103 a         3316 c         4804 g         6239 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcgact tcgggggata tgattatagc ggtaagccga
    22021 atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg tcactaactt
    22081 cggataacga aaagctacat tttacttctg atggtacagg aagcattatt atgtttacgc
    22141 gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg aaagttcgat
    22201 ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt acatcaccag
    22261 gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat gtagggaaag
    22321 aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta gatcgatttt
    22381 atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag ttgtacgaca
    22441 tcaaaatcga agaaggctca ac
//
LOCUS       DAZAOG010000026        22028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000026 DAZAOG010000000
VERSION     DAZAOG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.026"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACHDLD_000915"
     CDS             complement(61..1371)
                     /locus_tag="ACHDLD_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN3362668.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACHDLD_000916"
     CDS             complement(1383..2801)
                     /locus_tag="ACHDLD_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3362669.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACHDLD_000917"
     CDS             complement(3041..4609)
                     /locus_tag="ACHDLD_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN3362670.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACHDLD_000918"
     CDS             complement(4613..4894)
                     /locus_tag="ACHDLD_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN3362671.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACHDLD_000919"
     CDS             complement(4891..5814)
                     /locus_tag="ACHDLD_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN3362672.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACHDLD_000920"
     CDS             complement(5811..6689)
                     /locus_tag="ACHDLD_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN3362673.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACHDLD_000921"
     CDS             complement(6676..8013)
                     /locus_tag="ACHDLD_000921"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN3362674.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACHDLD_000922"
     CDS             complement(8016..9119)
                     /locus_tag="ACHDLD_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3362675.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACHDLD_000923"
     CDS             complement(9193..10701)
                     /locus_tag="ACHDLD_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3362676.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACHDLD_000924"
     CDS             complement(10667..11359)
                     /locus_tag="ACHDLD_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN3362677.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACHDLD_000925"
     CDS             complement(11381..12355)
                     /locus_tag="ACHDLD_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362678.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACHDLD_000926"
     CDS             12655..13527
                     /locus_tag="ACHDLD_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362679.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ACHDLD_000927"
     CDS             complement(14109..16307)
                     /locus_tag="ACHDLD_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362680.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ACHDLD_000928"
     CDS             16539..17531
                     /locus_tag="ACHDLD_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362681.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ACHDLD_000929"
     CDS             17591..19012
                     /locus_tag="ACHDLD_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN3362682.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ACHDLD_000930"
     CDS             19005..20504
                     /locus_tag="ACHDLD_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN3362683.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ACHDLD_000931"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ACHDLD_000931"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN3362684.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAZAOG010000027        21439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000027 DAZAOG010000000
VERSION     DAZAOG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.027"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..820
                     /locus_tag="ACHDLD_000932"
     CDS             119..820
                     /locus_tag="ACHDLD_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3362685.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="ACHDLD_000933"
     CDS             complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="ACHDLD_000933"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN3362686.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="ACHDLD_000934"
     CDS             complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="ACHDLD_000934"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN3362687.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="ACHDLD_000935"
     CDS             complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="ACHDLD_000935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN3362688.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="ACHDLD_000936"
     CDS             complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="ACHDLD_000936"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN3362689.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4352..5332)
                     /locus_tag="ACHDLD_000937"
     CDS             complement(4352..5332)
                     /locus_tag="ACHDLD_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362690.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5356..6840)
                     /gene="galT"
                     /locus_tag="ACHDLD_000938"
     CDS             complement(5356..6840)
                     /gene="galT"
                     /locus_tag="ACHDLD_000938"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN3362691.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6852..7841)
                     /gene="galE"
                     /locus_tag="ACHDLD_000939"
     CDS             complement(6852..7841)
                     /gene="galE"
                     /locus_tag="ACHDLD_000939"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN3362692.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8089..8256
                     /locus_tag="ACHDLD_000940"
     CDS             8089..8256
                     /locus_tag="ACHDLD_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN3362693.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8318..10129)
                     /locus_tag="ACHDLD_000941"
     CDS             complement(8318..10129)
                     /locus_tag="ACHDLD_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN3362694.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10126..10491)
                     /locus_tag="ACHDLD_000942"
     CDS             complement(10126..10491)
                     /locus_tag="ACHDLD_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN3362695.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10654..11049)
                     /locus_tag="ACHDLD_000943"
     CDS             complement(10654..11049)
                     /locus_tag="ACHDLD_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3362696.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11067..12029)
                     /locus_tag="ACHDLD_000944"
     CDS             complement(11067..12029)
                     /locus_tag="ACHDLD_000944"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN3362697.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12029..12241)
                     /locus_tag="ACHDLD_000945"
     CDS             complement(12029..12241)
                     /locus_tag="ACHDLD_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN3362698.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12262..12960)
                     /locus_tag="ACHDLD_000946"
     CDS             complement(12262..12960)
                     /locus_tag="ACHDLD_000946"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN3362699.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12980..13522)
                     /locus_tag="ACHDLD_000947"
     CDS             complement(12980..13522)
                     /locus_tag="ACHDLD_000947"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN3362700.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13654..14658)
                     /locus_tag="ACHDLD_000948"
     CDS             complement(13654..14658)
                     /locus_tag="ACHDLD_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN3362701.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14655..15644)
                     /locus_tag="ACHDLD_000949"
     CDS             complement(14655..15644)
                     /locus_tag="ACHDLD_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN3362702.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15641..16447)
                     /locus_tag="ACHDLD_000950"
     CDS             complement(15641..16447)
                     /locus_tag="ACHDLD_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362703.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16613..17569
                     /locus_tag="ACHDLD_000951"
     CDS             16613..17569
                     /locus_tag="ACHDLD_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3362704.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17646..18164)
                     /locus_tag="ACHDLD_000952"
     CDS             complement(17646..18164)
                     /locus_tag="ACHDLD_000952"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN3362705.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18252..18401)
                     /gene="rpmG"
                     /locus_tag="ACHDLD_000953"
     CDS             complement(18252..18401)
                     /gene="rpmG"
                     /locus_tag="ACHDLD_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3362706.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18629..19075
                     /locus_tag="ACHDLD_000954"
     CDS             18629..19075
                     /locus_tag="ACHDLD_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN3362707.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19270..21165)
                     /gene="asnB"
                     /locus_tag="ACHDLD_000955"
     CDS             complement(19270..21165)
                     /gene="asnB"
                     /locus_tag="ACHDLD_000955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN3362708.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6450 a         4551 c         3699 g         6739 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      901 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
      961 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1021 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1081 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1141 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1201 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1261 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1321 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1381 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1441 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1501 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1561 tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg
     1621 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1681 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1741 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1801 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1861 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1921 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     1981 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2041 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2101 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2161 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2221 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2281 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2341 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2401 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2461 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2521 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2581 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2641 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2701 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2761 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2821 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2881 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     2941 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3001 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3061 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3121 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3181 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3241 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3301 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3361 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3421 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3481 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3541 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3601 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3661 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3721 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3781 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3841 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3901 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     3961 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4021 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4081 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4141 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4201 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4261 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4321 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4381 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4441 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4501 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4561 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4621 aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg
     4681 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4741 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4801 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4861 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4921 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     4981 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5041 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5101 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5161 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5221 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5281 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5341 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5401 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5461 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5521 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5581 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5641 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5701 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5761 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5821 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5881 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     5941 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6001 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6061 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6121 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6181 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6241 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6301 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6361 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6421 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6481 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6541 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6601 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6661 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6721 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6781 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6841 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6901 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     6961 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7021 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7081 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7141 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7201 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7261 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7321 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7381 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7441 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7501 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7561 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7621 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7681 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7741 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7801 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7861 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7921 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
     7981 aacattttca agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc
     8041 tcttttgttc cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt
     8101 gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct
     8161 tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac
     8221 cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa
     8281 gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt
     8341 gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg
     8401 cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt
     8461 cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat
     8521 aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt
     8581 ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat
     8641 agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa
     8701 tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt
     8761 tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc
     8821 atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg
     8881 gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt
     8941 tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat
     9001 tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg
     9061 gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct
     9121 ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt
     9181 ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca
     9241 ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc
     9301 ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg
     9361 atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa
     9421 acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag
     9481 gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat
     9541 ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac
     9601 cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac
     9661 catcttttct tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa
     9721 attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag
     9781 attgctgatc tttttccaga cgattgattt cttctataat tagttgctta tttttggtta
     9841 ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct
     9901 gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa
     9961 cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat
    10021 tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt
    10081 ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc
    10141 catttcgata aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata
    10201 aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt
    10261 cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc
    10321 tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag
    10381 atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct
    10441 atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc
    10501 cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat
    10561 gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat
    10621 tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc
    10681 agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa
    10741 tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc
    10801 ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa
    10861 aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat
    10921 tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag
    10981 atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca
    11041 taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca
    11101 atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc
    11161 gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc
    11221 gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa
    11281 gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct
    11341 tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc
    11401 agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta
    11461 gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga
    11521 tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg
    11581 ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg
    11641 ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata
    11701 gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag
    11761 tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc
    11821 atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt
    11881 gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc
    11941 catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct
    12001 aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa
    12061 ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac
    12121 ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac
    12181 ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca
    12241 tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat
    12301 acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata
    12361 gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc
    12421 accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac
    12481 aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa
    12541 ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag
    12601 aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata
    12661 aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag
    12721 tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa
    12781 acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat aataagggat
    12841 ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc
    12901 ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat
    12961 gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct
    13021 ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa
    13081 aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat
    13141 tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg
    13201 attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat
    13261 aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt
    13321 ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta
    13381 tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt
    13441 tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta
    13501 atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa
    13561 aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc
    13621 tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct
    13681 gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg
    13741 tacttcccca cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga
    13801 accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc
    13861 agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct
    13921 tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa
    13981 tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc
    14041 tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc
    14101 ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat
    14161 cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat
    14221 tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc
    14281 taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa
    14341 gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga
    14401 atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc
    14461 tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa
    14521 agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt
    14581 accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt
    14641 ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca
    14701 atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta
    14761 aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga
    14821 tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca
    14881 ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac
    14941 cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa
    15001 gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat
    15061 actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc
    15121 tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata
    15181 gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa
    15241 ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa
    15301 tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg
    15361 attcctagta agcctgaatc tgctaaagga tttcttgtca ccccttgcat caatgcaccg
    15421 ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt
    15481 cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct
    15541 gctttttcca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca
    15601 aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct
    15661 cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa
    15721 tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg
    15781 taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag
    15841 catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag
    15901 tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca
    15961 agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc
    16021 caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg
    16081 cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt
    16141 atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg
    16201 ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat
    16261 tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag
    16321 attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag
    16381 acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc
    16441 tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat
    16501 ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata
    16561 tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt
    16621 aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg
    16681 ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag
    16741 tactcataca gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg
    16801 gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca
    16861 gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc
    16921 aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt
    16981 gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc
    17041 aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg
    17101 caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat
    17161 caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg
    17221 ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata
    17281 cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc
    17341 tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct
    17401 tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc
    17461 agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc
    17521 tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa
    17581 aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat
    17641 agattttatt cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga
    17701 tgggcatata gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct
    17761 gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc
    17821 cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag
    17881 atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc
    17941 gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga
    18001 agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt
    18061 gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg
    18121 ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat
    18181 gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa
    18241 ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat
    18301 ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt
    18361 tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt
    18421 acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt tttctagatt
    18481 gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta
    18541 cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt
    18601 atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc
    18661 aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt
    18721 agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg
    18781 gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa
    18841 gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt
    18901 agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca
    18961 ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc
    19021 aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata
    19081 tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt
    19141 cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg
    19201 aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt
    19261 tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac
    19321 aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc
    19381 tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc
    19441 ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa
    19501 ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag
    19561 atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt
    19621 acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc
    19681 caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg
    19741 atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta
    19801 atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc
    19861 tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc
    19921 aagtgattca tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc
    19981 taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa
    20041 ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc
    20101 tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac
    20161 gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa
    20221 cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt
    20281 tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc
    20341 tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac
    20401 ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc
    20461 gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg
    20521 aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc
    20581 tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc
    20641 ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc
    20701 aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct
    20761 tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat
    20821 cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc
    20881 atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata
    20941 gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc
    21001 gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata
    21061 ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt
    21121 ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc
    21181 taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac
    21241 gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt
    21301 acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt
    21361 ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac
    21421 acgacctaaa gatcgtgtc
//
LOCUS       DAZAOG010000028        21172 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000028 DAZAOG010000000
VERSION     DAZAOG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.028"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..671
                     /locus_tag="ACHDLD_000956"
     CDS             <1..671
                     /locus_tag="ACHDLD_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3362709.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVA
                     IFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRK
                     KRNI"
     gene            complement(1024..1872)
                     /locus_tag="ACHDLD_000957"
                     /pseudo
     CDS             complement(1024..1872)
                     /locus_tag="ACHDLD_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2025..3137
                     /locus_tag="ACHDLD_000958"
     CDS             2025..3137
                     /locus_tag="ACHDLD_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3362710.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3215..3508)
                     /locus_tag="ACHDLD_000959"
     CDS             complement(3215..3508)
                     /locus_tag="ACHDLD_000959"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN3362711.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3676..3990
                     /locus_tag="ACHDLD_000960"
     CDS             3676..3990
                     /locus_tag="ACHDLD_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN3362712.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4011..5990
                     /locus_tag="ACHDLD_000961"
     CDS             4011..5990
                     /locus_tag="ACHDLD_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3362713.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6003..6329
                     /locus_tag="ACHDLD_000962"
     CDS             6003..6329
                     /locus_tag="ACHDLD_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN3362714.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6345..6863
                     /locus_tag="ACHDLD_000963"
     CDS             6345..6863
                     /locus_tag="ACHDLD_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362715.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6863..8215
                     /gene="celB"
                     /locus_tag="ACHDLD_000964"
     CDS             6863..8215
                     /gene="celB"
                     /locus_tag="ACHDLD_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN3362716.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8299..9804)
                     /locus_tag="ACHDLD_000965"
     CDS             complement(8299..9804)
                     /locus_tag="ACHDLD_000965"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN3362717.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9896..10486)
                     /locus_tag="ACHDLD_000966"
     CDS             complement(9896..10486)
                     /locus_tag="ACHDLD_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3362718.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10665..12311
                     /locus_tag="ACHDLD_000967"
     CDS             10665..12311
                     /locus_tag="ACHDLD_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFN3362719.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12360..13079
                     /locus_tag="ACHDLD_000968"
     CDS             12360..13079
                     /locus_tag="ACHDLD_000968"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN3362720.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13558..14505
                     /locus_tag="ACHDLD_000969"
     CDS             13558..14505
                     /locus_tag="ACHDLD_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362721.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14517..15437
                     /locus_tag="ACHDLD_000970"
     CDS             14517..15437
                     /locus_tag="ACHDLD_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362722.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15478..16935
                     /locus_tag="ACHDLD_000971"
     CDS             15478..16935
                     /locus_tag="ACHDLD_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3362723.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17280..17894
                     /locus_tag="ACHDLD_000972"
     CDS             17280..17894
                     /locus_tag="ACHDLD_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3362724.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17887..19611
                     /locus_tag="ACHDLD_000973"
     CDS             17887..19611
                     /locus_tag="ACHDLD_000973"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3362725.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19608..21059
                     /locus_tag="ACHDLD_000974"
     CDS             19608..21059
                     /locus_tag="ACHDLD_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3362726.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6823 a         3584 c         4274 g         6491 t
ORIGIN      
        1 tcctgcaagg agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc
       61 taaccacagg agcagatgga aaactggcga ttgacggctt agcaccagga gtgtatcagc
      121 tggtagaaac ccaagcgcca acggggtatg aattggatgc gacaccaatc gagtttgaga
      181 tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaatcgactt acaccaggcg
      241 gtgtcgtgtt gacaaagatc gatgaccaaa gcggagaagt cctgcaagga gctgtttttg
      301 agttacaaga tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa
      361 aactggcgat agatggctta gcaccaggag catatcagct ggtagaaact caagcgccaa
      421 cggggtatga attggatgcg acaccagtaa ctttcaagat cgaaaaagaa caagaagtgg
      481 cgatcttttt aacaaaagag aatagaaaag ttgctgatag ctcagatata agaggaaaca
      541 atccaacaag cagtggaaat aagcatcttc caaaaactgg agaaaccatc attagccagt
      601 ttatattgtc aatattgggt gtgctacttg ttttcatcag tataaaattt agaaagaaac
      661 gaaatatatg agctaaaata aaaaattgaa taagcatagt ttttaaaatc aactagtacc
      721 catcaattga atggaaacta ccgattttag aaggggtata caaacaaata ttagtatcgg
      781 agtagagaag gttgaaccca accagtaaat aagtaaagac aattttgtgc tgcgttgaga
      841 agtgaaaaat aaacctttgt agtaatacta gttaataaaa aatatacaaa aacactgcga
      901 aaatcattct tgagtttcgc agtgtttttt gcctaatgac tcaagtttat tgggtaatac
      961 tttgattttc tttctacaaa taatcagcga tcagttgtaa ttttttctct agttttaatg
     1021 gctctttgtc aataaggaga aaaacaagtc gggtcttttg tctcggaaag cttcaaccaa
     1081 ctcgtgaata aaggttacgc ctccttgaaa ggcgtggaaa aaccaagtaa tcgatcaatc
     1141 accaatttcg catttggtag aacatgtttg gtgagctgaa agtaggtggc gttcatgtct
     1201 gtaactaaga attctatttc tttaggatta gtgcagctaa ggaaatagtt tgttaatcga
     1261 ggaatttttt gtgtaggtaa attttctcga ttaatttttc tgtctcgcca tccgcggaaa
     1321 taaaactcag tttatcctca acggaaacgt gagaacgaaa ttcacaaccc tcaacactca
     1381 agacagaatt cttttggaag actttggtaa atagcttctc aactctttca atgtgcgaat
     1441 aacggttgtc aaagatacct aacaactttt cgaaataaaa aataacgaca ctttttcagt
     1501 cagtaaagaa gtgattttat gtctgacatg atttgaaatc gagtgccttg gacggataaa
     1561 ataactttga gcgttccaat ggcttcgaca gtttttacag gtatagcgct gcttttttag
     1621 gcgtataacc attggcatat gattatatta ttcaaagtgg acaatggttt ccttcttctt
     1681 gtttttcacc accattaatt tcctatttcc atctaccaca gcagaaccac aatttctaca
     1741 gacacatgga gcagggaaga gaactgcagc aacgatcaat gtttttttct tctgtaaggt
     1801 ttcgtaagag acctctgtaa tcatcaaatt tttgtctatt gatctcagaa tttttttgat
     1861 agaaccattc ataaagcata tcgttctctc agttgtttat tttgtggtga tttaatcata
     1921 caagaaaacg atatgttttt ttataaaaac atgaatagga aaaatagttg acaagaaaat
     1981 gacaacgcca tatgatgttc catagtattt acaacctgtc atagatgact gtaaaaaata
     2041 aaagtaaaaa gaaaggacga caacaggtgg atttttatca gtctctacaa ctagatccgt
     2101 ttattttaaa acaaaaaata cgagaagcag cttctaaaaa agaaaagtgc atgtatattt
     2161 gttcattgtt tttacgatca ttattcatcg tattgtttgc catttgtttt atcattataa
     2221 ttacaacatt atttgaatcg aagcataaac catacgctgt ggttttattc tgtatgttga
     2281 tgagtattcg ttttgttgac tttggttata agatttccca ttcgatcata ggtttagcaa
     2341 tcgtcatgtt ctcactatta gtagcaccgt atgtccagct gatcaaatgg tcagttatgg
     2401 gcatgttgat tcattttatt ttactaagca gcatattaat ggcaaccgct agtgatccca
     2461 aaatggggaa tgcttctttg tacggctttt catatctctt tattgtttat tcgttaccta
     2521 aagattcgtt gaacaagaat ttctttacac aaacaagtag cttattattt ttgttttttt
     2581 gttggttttc agttctcctt tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt
     2641 ttaaagagat ttttttgaaa ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg
     2701 catttggcat tagtcttttg atcgttgcag gagagtatgt cccttttcag cgattgatgt
     2761 gggcagggtt tgcattttct tctattgtat ctagctatgg attgatgtcg attggtttca
     2821 aagaacgagc agtagaccga atcattggct cattgattgg atgtgtttta tttattggta
     2881 tcagccaatt tattcctttt aactgggtcg gtatattagg tggtctagct ttaggtgttt
     2941 gttctaccta tagatataaa acagttttta attcttttgg tgctttggca atcactgcca
     3001 gtttattcgg ggttccagga gcagtaacga tccgcatttt tgagaatatc cttggggtat
     3061 gtttaggaat catgtatatt ggggtgactg aaatacttat ccgaaaaata agagaaaaac
     3121 acggtttgaa tcattagatg agatcgaata aaaaataagg aaaaagtgaa gatacacatg
     3181 gatgataaat tgctatcatt ccatgtgtat ctttttacgg taaatcagtg aaacgttcga
     3241 ctttcatttt taatttatac tttttacgga gttcgtttat ttgagccatc atttcggatt
     3301 tgtgatgctt ttcgattgct tcttcatctt tccaacggtc gatcagtaaa agagattctg
     3361 gatcttctag tggaaagtaa tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt
     3421 tgacaatgtt tctttcgacc atttcttctg caaattttct ggcttgtcca tttgtacttg
     3481 tatacaaaat attcactgtg atactcacta aatcccctcc tgaaaaaatt ctatcactag
     3541 tatatcacgg tgccagaaat tattattcaa gatatgatac acattttttc cgcaatgcta
     3601 cggaaatata tattttgaat agaaaacgct tacaagatat gcttggttta taaaaataaa
     3661 aggagtgatt gatttatgaa aaaagcattg attatttgtg cggcaggaat gtcatcctcg
     3721 atgatggcct caaagacaac ggatttcttt aagcaaaaaa gagaagaggt actcgtagat
     3781 gcagtttcag ctacagaagg cgataatatg attaagaata gtgattttga tttatttctg
     3841 atcagcccgc aaacgacgat gtttttggat aaattcaaaa aattaggaga atctgttgga
     3901 aagcctgttg ttagtatccc tttccaagcg tatgtcccaa tccctacagg catccagaag
     3961 ttagcagagg tgatcgaaga gaacatatga tggaatagga gtggatgata atgaaacaga
     4021 aggaaaaatt gctgttgaga tatttgattg atcacaagaa ggagtatgtc accagtcaac
     4081 gtttagcttc tgaattgtta ttatctgatc ggacaatccg taattacttg cagcggatca
     4141 aagagttagt agaaaaaaac ggaggtaaga tcattgctaa accaggttat ggctaccaac
     4201 tgcatatttt acaaagactg acatttgact tgtttttgag tcggcaagaa atagtacctg
     4261 gatatgctag agaaccgcaa ggattttatg agtccgaaga tcgacaaaag tatatattga
     4321 accaattgct tttggaagat gccattcttt ttatggatga tttggcagaa gagctttata
     4381 tcagtcgttc cagtttgaca aaggacatgc aagaaatcaa ggagcgattg attccatatt
     4441 cactaaaaat cgtttctaag catggtcaag ggacatggat cgaaggggaa gaaagaaatc
     4501 gacggcattt tatcatggat actttttttg gaaaccatta tggaaattca ttgaaagaat
     4561 atttagggca aagccgtttt ttcccagaca tcagttttga agagctagtc attatcattc
     4621 tcgatgaaac aagagaagct aagttaaaag tttcggattt tatcatacag aatctgactt
     4681 tacacttggc tttggggatc aaacggctcc gagaagggtt tgaaatcaag gaattaggca
     4741 tcgaacaaga agtttctaag cgagtagagt atcaagtggc gcagcggatc gttcaacgta
     4801 ttgaggtaat cgcaggtgta tcgtttcccg aagaagaaat tgcttatttg acattacatc
     4861 tcatggctaa gtcgaatcac aaagaaaatc aaaatgatca ggaactatca aaggaattag
     4921 cgaaagttct gaaaagattg tctcaggaat ttcatttttc attagtagag gatctccagc
     4981 ttttcaatgg actactcgat catttgtcac cgatgatgat tcgattgagc cgaggtattc
     5041 gcattgagaa cccattgact gaggagataa agaaagaaaa tcccaaagtc tttgacgcag
     5101 taaaaaggca tttcagcaac atgccggctt tgaaaaacta taccatcaat gaagatgaat
     5161 gggcctattt ggctttgcat ctcatggcgg cattggaaaa agaaagagct gcgcataaac
     5221 tacatgcatt gattatctgt gcaacgggtt acggcagtgc ccaactatta aagaatcgcg
     5281 tagtcagtga attcggtaaa aatattacag tggtaagtgt aaaaggctat tatgagatca
     5341 atgaaacaac tttaaaagga atcgacctga ttatttcttc tattgatctt tcgacaatgt
     5401 ttttcaaaat tccagtttta catgtcagta tttttttgaa tgaagatgat gttcgtaaaa
     5461 tacgaaaggt agttggagaa tctataccct atcattcctc agatagaccg ataccagcat
     5521 tttctctaca gcaaaaaaag cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga
     5581 cttatacgta cgaaccgaaa aaggaagaaa ttcttcatga tttgctgcaa aagctgagca
     5641 taaatgaaga agaaggatat ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata
     5701 tgggacaaat catattcagt gatacagtgg cagtcccaca tcccgctgtt ccagttggaa
     5761 tcgaaacaaa ggtagccgtt gcattgattc cttctgggat gcagtgggat caatatcaag
     5821 aaattcattt tgttttttta gtttcgcctt cttatgtcga aaatgaagga attacggtcg
     5881 tgactaaagc aatcgttagg ttagttgacc agctaaaagt acagcaggaa atcttagctg
     5941 aacctatttt tgagaatttc agcagtaaat ttatcaaact gatcgaataa ggaggaaaac
     6001 agatggctga aaaaataaac agtgaaacac tacaagtgac ttcttttgat atcattttcc
     6061 atagcggaaa tgcacggaca ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt
     6121 ttgaagaagt agaagagaag ttgcgacaag caaatgaaga gattcttcat gcgcatcagt
     6181 cacaaactca acttctgaaa gaatatgcca gcgggacaaa aatagaaatg gaaattatca
     6241 tggtccatgc acaagatcat ctgatgacga cgatgacatt attggaagtt gcacaggaaa
     6301 tgaattattt gtatcaaaaa aataaataga aaggaaggat cattgtgcag gatacagtaa
     6361 aaaggaaaat cgaattagaa aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt
     6421 atctaatcgt acgttacctg actgcctttt tctttttcat gaacatgcaa tggatgattc
     6481 tgcttctatc agctaaaagt ttaggaagta gtttaccgat gattctgatt ttagctattt
     6541 tgcctgcagt aggagaacaa gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga
     6601 caaagtggta ttttttgttc cagagtgtct ttaatttatt agtgattcca gtgttgttta
     6661 cctcaggatt tacattcttg tatcctttta tggcaaataa caatggagga cagttatttg
     6721 ttttcattct tgttgtaagc ggtatttttg ccagtgcatt tatacagtat cgtttaaaga
     6781 aaatttcact caaccaagac caacagtata tccgtattaa acaatatgaa aaagcgttat
     6841 atttaggaaa ggagaataac taatgtttga ttttttgcag aaatacctga tgggacctat
     6901 ggggaaaatt gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga tggcttctat
     6961 tcctttcacg attgtaggct cgatgttttt ggttttaaat attttgccat taccgttccc
     7021 atttttagaa ggatttttta atgcaacttt tttcaaagtt agtgacttgt acatgatcgt
     7081 caatacaatg acgatgggga ttttatctat ttactttgcc attgtgtttg cttatgaatt
     7141 aacgtcaatt gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg gggcattact
     7201 atctgttttt gcattcttta tgtgtatccc tgaattgatc atgactgatg gtaaaatcag
     7261 cttgattgct tctatcaatg agacagaaac tgtagtaaat ggtattagga tgggctcttt
     7321 tgtcgaacgt ttaggaactt caggaatctt tacagctatt attatgtcga ttattgcggt
     7381 cgaattgtat tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag atgttgttcc
     7441 gccaggagtt tctcgttctt tcactgctct gatcccaaca tttgtcattg cgtttgtggt
     7501 gatgatcatc aatggattat tagttgcact aggaacggat atcttcaaaa tgattgctat
     7561 cccatttggg tttgttacgc agttgacgaa tacatggctt ggtgtcatgg tgatttattt
     7621 cttgatccat gcattgtgga tcgtagggat ccatggagcg aatatcatca cttctttctt
     7681 gaccccgatc gtattagcta atatggcagc taacgcacaa ggcgcaaatt atccgttggc
     7741 aggagaattc aacaattctt atgtaaccgt tggtggatca ggtgcgactt taggattgat
     7801 catctttatt gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa aagcctcact
     7861 tgtaccagga ttattcaata tcaatgaacc aatcattttc gggatgccaa tcgtctacaa
     7921 tccctattta gcggttccgt ttttcttagc tccgatggct tctgctacac tcgcttattt
     7981 tgccattcgg ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc cttcaccagt
     8041 cgggatcgga gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat
     8101 ttgtgcaatc atcgcattca ttatctggtt cccatttatc aaattctatg ataataaatt
     8161 attaaatgaa gaaagagaaa aggcagcaga attagctagt gaaaagttaa aatgatttct
     8221 aagaaaaaag gaactgtgac aaaaaatcac agtccctttt tttcttagaa atgggaaacc
     8281 actagtatat aaataccatt attcttcaat caaccgcttc acgattgcat aatccccttc
     8341 ataaggttca tctacaccat tgattttttg atagggatcc acgccttttc ccgcaataac
     8401 gactgaatct tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag gacgatccat
     8461 ttcataatga acgatcaaat ctggattagt aattgcttca ttgatttctt ccgcgatttt
     8521 tttcggatct tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga
     8581 tagcactttt ccaaaatcat gtcgacggga gattgctttg tttcctgggc tgcctaatac
     8641 aacaattaca cggccatctg gatgttcatt tttggcaaat ttcagcaatg ttttcaagct
     8701 tagataatta tgtgcatagt ccacatagac atgcgctcca ttggattgga tcaattgatc
     8761 cattcggcct ggtacacgag cttcttttaa tccgtcttgt gcatcttgac tggaggcacc
     8821 tgttagtgct gcggcaatga tcgcactggc tgcattttct tggttgaatc ctccagccag
     8881 caaaatatca taagcagtat cgcagatagc taagcgatct tcatgagaag ataaagtaaa
     8941 accgtgagta cctttatctg aacggatcac ttgataattg ctttcttccg aacgaccata
     9001 agtgatcgtt ggaatctgga atagatcggc tgtttcccta agtaaatcaa aatagtcact
     9061 ttcatgattt aaaacaactg tttttgaatt caagatcaat tgtcgcttgc aatagaaata
     9121 gtcatcaaat gtcggatgtt cgatcggact gatatggtca ggcgagatat tcaagaagat
     9181 tccgacatct aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc
     9241 catcactaga tgagtcatac cattttcaac tgcttctgac atcatacgat ataaatctaa
     9301 tgactcaggc gttgtcaaat gggatttgaa atatgttttg ccatctaacg ttgtattcat
     9361 agtagaaagc aatgctgttt ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta
     9421 cgcagcagtt gttttacctt ttgttccagt gaatccgatc actttcaatt tattttgcgg
     9481 atagtcgtaa aaagccatag caagaagcgc cattgcttta cggatatccg ttacgatgat
     9541 tcctactgct gaattttcgt atggttgttc tgatacataa tattgaatac cctgttcgat
     9601 tgctgaatcc aaatattctt ctttgaaatt cattccttta cagaaaaaca aagtagaagc
     9661 atcagctttt cttgaatcat aacttaactg aagaaattca gctttttctg gtacatcaag
     9721 atgccagcct cttgttgaaa caaattcttt taatagattt tcttttaata gtaaatgacg
     9781 gatgtcttct aaagagaagc tcattgattg atccctcgct ttattcaatt tcttggttta
     9841 ttatagcaca ctctttttaa tatatcgttt gtttccgcta tgacaaaatc cagctttatt
     9901 caaattttct tgacacaggg gacaagcgga tgagtatttt cataaacaat gaactgactg
     9961 taatagatag gagaacgaat agtgtctcca acaatccaaa aacataataa ttcaaaaaga
    10021 cgataagact attggtaagt aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga
    10081 aaataatcgg cggtatcgtt ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa
    10141 ccccaagact gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt
    10201 ccacttttgg aacaatctct aaaacaacag gaaaaagcag acttcccgct acagtcttaa
    10261 aaaatactga ttttcctaat aggacagcgg ctagaagcag catcataaga ttgatgctcc
    10321 aaacagtaac tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg atggcgccca
    10381 ctccggcgga agctatatcg tgagggagca agaaccaatt caatccaact gtgataaaga
    10441 aaagacctaa aaatataacg ggtagttttt ttatttcttg tgtcattgat atctcctata
    10501 aatgttagcc ataaaaggct gtatatttct ttactttacc aaaaaaacga aaccgaattc
    10561 aaaaaaacaa ttgttcttat aaaagttata taaaatcgat tgccagtttc tgttttacca
    10621 attaaaatga tagtaatgtt ttgtagaaag ttggaatctg accgatggat aatcaaagaa
    10681 aaccagcatt agatcaatta acaccgcaag aaaaaatggc acgaggctct gcttggatga
    10741 ccgccagtaa tatcatttca agattgctag gcgctgtcta tatcattccc tggtatgctt
    10801 ggatgggcga aaatgctaag gctgccaatg gattattcaa tatgggatac aatatatatg
    10861 cattgtttct actgatctct acagcaggaa tacctgctgc gattgcaaaa caaactgcac
    10921 gctataattc attgaatgaa tatggcactt cacgacgatt atttatccgt gcattacaga
    10981 tgatgggcgg gttaggcctg ttgtttgctt tgttcatgta catcgcctct ccttggctag
    11041 ctcatgcttc tggtggcgga gaagaactga ttccaacgat gcgttctttg agtatcgctg
    11101 tattggtttt tccttgtatg agtgtgatac gagggtattt gcaaggcaat caagaaatga
    11161 tgccttatgc gctgtcgcag attgtagaac aggtggcacg tattttttat atgctattgg
    11221 ctacattcat catcatgaaa gtcatggatg gtgactatgt gacggcggtc acgcaatcaa
    11281 ctttcgcggc atttatcggt atgctagcaa gtattcttgt ccttttgtat tttttgaaga
    11341 aacatcaagc gtatacatcg gcgtttattc actacagcga aaacaaaata aatattgcaa
    11401 cgaaggaatt gttgttagat accgttaaag aagcgattcc gtttattatt gtgggttcag
    11461 gggtaacgat cttcaaatta gtcgatcagt tcacttttat gaagatcatg tctgcatcca
    11521 ccgagtattc caatgcacag ttactggatc ttttttcgat ttttagtgcc aatccagata
    11581 agttgacgat ggtagttatt gcattggcaa catcgatcgc gtctactggg ttaccgttga
    11641 ttacagaagc tgttacgttg aaagatcgag taggtctggc aaaattgaca agcggaaatt
    11701 tacagttgtt ttcatttttt atgtttccgg cgacatttgg tgttatgtta cttgcctatc
    11761 cgctaaatac attattttat acaccggatt ctctaggaag taatgtactg atccaagcaa
    11821 gttttgtagg actcttccta ggactatata tgctagtttc caacatgctg caaggaatgt
    11881 ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt tctagtgaaa ctccttcttc
    11941 aatacccagc cattcgttta tttgaagtat acggcccttt attggcgaca atgatcggat
    12001 ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga agtagcccgc tttaaaccaa
    12061 gttttgtctg gcgcaggaca ttgctgatct ttattcttac cttgattatg cttttggctt
    12121 cgtggatcac aaagatgatt tttggtgctt ttttaaacga agatagcaaa ttccagtctc
    12181 tattgttgat tcttgtagta gcaggtatag gggctttagt gtatggttat ttagctttga
    12241 aaatccgctt ggcagaaaga ctactaggac caggaatagc gcgtttacga cataaattaa
    12301 aaataaaata agtgaatcag aagagctaga ttgatgctct tttgaaatgg gggaaacgca
    12361 tgcgtttaga taagttttta gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat
    12421 taatcaaaaa aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag
    12481 taaatgaatt cgaagatgcg gttacttatt taggagaaat cattacgtat caaaaagatt
    12541 tttactacat tttgcataaa cctgcaggag tcatttctgc tactcaggat aatcatgatc
    12601 aaaccgtaat cgatttattg gaagaccagg attatcggga agatcttttt ccagtcggcc
    12661 gattagataa agacacagag ggcctcttga ttttaacgaa tgacggggtg tttgcccatc
    12721 agttactatc acctaaaaaa catgtggaga aagaatatct agctgaggtt agcggtataa
    12781 tgacagaaga agatatccgt ttatttgcag aaggactgat tattgatggc gatgagcaga
    12841 cattgccggc aaaactccaa attgatcaag tagatcaaga gacagaaact tcaattatcc
    12901 gtctgatttt gcatgaagga aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa
    12961 aagaagtctt atatttaaaa agaatccgta tgggaaaatt ttggctgcct aaagatttga
    13021 ataaagggca atatcgaccc atgaccaaag aagagctaga acaagtgaga gaaaaataat
    13081 tgaatggata gctaaaagaa taagcagagt agttgtagcg ccatctgaaa agcagacttg
    13141 tccgtctgac tttcggatgg cttttttgtg aaatttcggc ttatttccgt agaagctact
    13201 tctggaacac cgtttgttcg gaataaggga ctgtgacaag acttttgtta cagtccccat
    13261 tgttccttgt gactcagagt tagatagtaa ggacacgatc tccctaatac tcaggccaaa
    13321 acgctgaatt cacaacctct gataaacggt gttcaatcag ctgaccctcg gtattagctc
    13381 aaaaaacggc aaaatatgag gaactatttt cccgtttttt tctctaatac tcaggccaaa
    13441 acgctgattt cacaacctct cctatacttt gtttcatttt ccctatactt tgtttattta
    13501 ttctcattat tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg tttttagatg
    13561 aaaaaacgaa aggggttcaa tcattttttt atgaatctat ggcgttataa agcacttgtc
    13621 ctgatggctg taccgggtat gctttggatg atttttttct tttacatacc tgtaatggcg
    13681 aatgtcgtag cattcaaaaa ttttcatatt tcagcagatg ggttcttggc tagcttgagg
    13741 gaaagtcctt gggtgggttg ggaaaatttc aaatttttat tttcttcgga tcaagcattt
    13801 ctcatcacta aaaatacaat tttatataat gtagcattta tccttttgaa tctgctgatc
    13861 tcagtcgtat ttgcaatcat tatgagcgag ttgagaaata aacgactcgt taaagtttat
    13921 caaacgatgt ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac
    13981 gcttttttaa gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa
    14041 gctgtcaatt ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca
    14101 tggaaaggga ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg
    14161 acttattatg aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg
    14221 acgataccac aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt
    14281 ttccgagctg attttggcct gttttataac attcctagga attccggtgc attatatgat
    14341 gtgactcaag ttttggatac gtatatctac aatggcctga catccacagg agattttggt
    14401 atgacggctg ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca
    14461 aatacgatcg cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga
    14521 aaaaaaagaa agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct
    14581 ctaatataat gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg
    14641 ccatctcttt taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa
    14701 agttcagtat atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag
    14761 cgcttttcgt cactgtactt gttactgttt taggtacact gatcaatagc acggctactt
    14821 ctttatatgc ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt
    14881 tttgtttgat tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa
    14941 atcttcttca gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct
    15001 tcaatattat tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat
    15061 cggcacggat cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag
    15121 ctgttccagg tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt
    15181 tcaatgcttt actatacatc caagatgata agctagttcc actacagtat cttttgatga
    15241 aaatccaaag taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag
    15301 gcggattagc ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt
    15361 tgcctattgc gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg
    15421 gcggagtaaa agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg
    15481 aagttatgga agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca
    15541 gcatgcggag gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag
    15601 atgtatcaaa tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa
    15661 cgaatcgaaa aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac
    15721 gaaaagaaaa tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac
    15781 aactatgttt ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag
    15841 tacgctgaaa aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat
    15901 ggaaaattat atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt
    15961 aacaaagctt tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat
    16021 gcagaaaaag tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt
    16081 ggacaaggct tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt
    16141 gctattgatc tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat
    16201 atcggactgt tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct
    16261 gcaacgagca ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca
    16321 caaggaccgt atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc
    16381 tcaaaagcaa ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc
    16441 gtatcaaata cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt
    16501 gatccagaat tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc
    16561 gatggttcag atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct
    16621 gcttggaata caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg
    16681 attgccaaac gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc
    16741 ttcaacactg acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat
    16801 ttagatggat tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac
    16861 gctttggaca aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag
    16921 ttccgacaag aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac
    16981 ctcttgttcc gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg
    17041 taaaaatgtg gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat
    17101 ttcacaaact cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac
    17161 gacaaaatat gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga
    17221 ttgcccaacc tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata
    17281 tgttagggaa ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct
    17341 tgtatttctg gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga
    17401 aaacagtcaa tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa
    17461 gaggctggaa tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt
    17521 tatctggtac tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca
    17581 tttttttagc tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc
    17641 aatacagtat gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa
    17701 gttttatttc ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta
    17761 taattttagg cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt
    17821 tgacggtttg gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg
    17881 aaagttatga ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg
    17941 cggaaatacg cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc
    18001 catacttggg aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc
    18061 acaagcagaa tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta
    18121 gggaattccg aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat
    18181 ctgatgtatg tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa
    18241 atcaaagatc tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa
    18301 ctaccggagt attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca
    18361 ccaaaattac tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca
    18421 ggaagtattt ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg
    18481 tcattcaatg gactctcttt atacatgatt tctggaacga caaatcgact atacacattc
    18541 catgataata aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt
    18601 ctgaagaaaa attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac
    18661 caagaactgt ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc
    18721 gaaacgggaa aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg
    18781 cttatctttc tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag
    18841 gccatggatc aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa
    18901 tatgaactga gggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt
    18961 gtagaagaca tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa
    19021 gcacagatca atccacactt tttatataat acattagaat atatccgaat gtatgcaatc
    19081 agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac
    19141 aatacgaatc aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat
    19201 gtctatttgt atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct
    19261 gatttggaaa agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt
    19321 aaacacggta ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa
    19381 ggcaaacggg taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta
    19441 aaacagattg aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg
    19501 caaaacgtga atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag
    19561 aataatgaga caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc
    19621 aagtgttatt ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt
    19681 ggcaaagttt aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg
    19741 tacttgaaca gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg
    19801 gactggaatt tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat
    19861 caggatacca agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt
    19921 tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt
    19981 tagatcagca aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc
    20041 aatggttgaa tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg
    20101 agaagcagca taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata
    20161 gatggttgca agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat
    20221 tgatcacgtt gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa
    20281 actttcctga tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa
    20341 tccctgaaac attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata
    20401 aagacgagca tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt
    20461 caagtttcag acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg
    20521 atttttttga acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt
    20581 ttttactgtt tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac
    20641 aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt
    20701 cattcatcaa aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg
    20761 taatcctgca ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc
    20821 atttaaatgt catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag
    20881 cttatttgaa ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa
    20941 acatcaatga gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat
    21001 tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa
    21061 agaaaaagcc aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc
    21121 tgttcttatt acttgaggca aggcagcttt ttcatgacct ctcgataaac gg
//
LOCUS       DAZAOG010000029        20824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000029 DAZAOG010000000
VERSION     DAZAOG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.029"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHDLD_000975"
     CDS             591..1280
                     /locus_tag="ACHDLD_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3362727.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHDLD_000976"
     CDS             1267..2475
                     /locus_tag="ACHDLD_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN3362728.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHDLD_000977"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHDLD_000977"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN3362729.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHDLD_000978"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHDLD_000978"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN3362730.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHDLD_000979"
     CDS             complement(6578..7273)
                     /locus_tag="ACHDLD_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN3362731.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHDLD_000980"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHDLD_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN3362732.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHDLD_000981"
     CDS             complement(8472..8663)
                     /locus_tag="ACHDLD_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN3362733.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHDLD_000982"
     CDS             complement(8684..9574)
                     /locus_tag="ACHDLD_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN3362734.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHDLD_000983"
     CDS             complement(9561..10328)
                     /locus_tag="ACHDLD_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN3362735.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHDLD_000984"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHDLD_000984"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN3362736.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHDLD_000985"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHDLD_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN3362737.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHDLD_000986"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHDLD_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN3362738.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHDLD_000987"
     CDS             14899..15282
                     /locus_tag="ACHDLD_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362739.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHDLD_000988"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHDLD_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN3362740.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHDLD_000989"
     CDS             complement(16226..17044)
                     /locus_tag="ACHDLD_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN3362741.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHDLD_000990"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHDLD_000990"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN3362742.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHDLD_000991"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHDLD_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN3362743.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHDLD_000992"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHDLD_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN3362744.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..>20824
                     /gene="dnaN"
                     /locus_tag="ACHDLD_000993"
     CDS             19704..>20824
                     /gene="dnaN"
                     /locus_tag="ACHDLD_000993"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN3362745.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVR"
BASE COUNT         5939 a         4249 c         3824 g         6812 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacg
//
LOCUS       DAZAOG010000030        20726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000030 DAZAOG010000000
VERSION     DAZAOG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.030"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            169..2097
                     /locus_tag="ACHDLD_000994"
     CDS             169..2097
                     /locus_tag="ACHDLD_000994"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3362746.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2235..2690
                     /locus_tag="ACHDLD_000995"
     CDS             2235..2690
                     /locus_tag="ACHDLD_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN3362747.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2770..2898
                     /locus_tag="ACHDLD_000996"
     CDS             2770..2898
                     /locus_tag="ACHDLD_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362748.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3160..3429
                     /locus_tag="ACHDLD_000997"
     CDS             3160..3429
                     /locus_tag="ACHDLD_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362749.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3829..4083
                     /gene="rpsT"
                     /locus_tag="ACHDLD_000998"
     CDS             3829..4083
                     /gene="rpsT"
                     /locus_tag="ACHDLD_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN3362750.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4172..5218)
                     /gene="holA"
                     /locus_tag="ACHDLD_000999"
     CDS             complement(4172..5218)
                     /gene="holA"
                     /locus_tag="ACHDLD_000999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN3362751.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5256..7529)
                     /locus_tag="ACHDLD_001000"
     CDS             complement(5256..7529)
                     /locus_tag="ACHDLD_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN3362752.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7522..8061)
                     /locus_tag="ACHDLD_001001"
     CDS             complement(7522..8061)
                     /locus_tag="ACHDLD_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN3362753.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8197..8415)
                     /locus_tag="ACHDLD_001002"
     CDS             complement(8197..8415)
                     /locus_tag="ACHDLD_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN3362754.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8412..8657)
                     /locus_tag="ACHDLD_001003"
     CDS             complement(8412..8657)
                     /locus_tag="ACHDLD_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3362755.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8773..9501)
                     /locus_tag="ACHDLD_001004"
     CDS             complement(8773..9501)
                     /locus_tag="ACHDLD_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN3362756.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(9660..10703)
                     /locus_tag="ACHDLD_001005"
     CDS             complement(9660..10703)
                     /locus_tag="ACHDLD_001005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN3362757.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(10693..11184)
                     /gene="coaD"
                     /locus_tag="ACHDLD_001006"
     CDS             complement(10693..11184)
                     /gene="coaD"
                     /locus_tag="ACHDLD_001006"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN3362758.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(11181..11741)
                     /gene="rsmD"
                     /locus_tag="ACHDLD_001007"
     CDS             complement(11181..11741)
                     /gene="rsmD"
                     /locus_tag="ACHDLD_001007"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN3362759.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(11860..12198)
                     /locus_tag="ACHDLD_001008"
     CDS             complement(11860..12198)
                     /locus_tag="ACHDLD_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN3362760.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(12182..12628)
                     /locus_tag="ACHDLD_001009"
     CDS             complement(12182..12628)
                     /locus_tag="ACHDLD_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3362761.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(12609..13733)
                     /locus_tag="ACHDLD_001010"
     CDS             complement(12609..13733)
                     /locus_tag="ACHDLD_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN3362762.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(13754..17182)
                     /locus_tag="ACHDLD_001011"
     CDS             complement(13754..17182)
                     /locus_tag="ACHDLD_001011"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN3362763.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(17208..18371)
                     /locus_tag="ACHDLD_001012"
     CDS             complement(17208..18371)
                     /locus_tag="ACHDLD_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN3362764.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(18374..18676)
                     /locus_tag="ACHDLD_001013"
     CDS             complement(18374..18676)
                     /locus_tag="ACHDLD_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN3362765.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(18790..20562)
                     /gene="recQ"
                     /locus_tag="ACHDLD_001014"
     CDS             complement(18790..20562)
                     /gene="recQ"
                     /locus_tag="ACHDLD_001014"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN3362766.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         6001 a         4299 c         3566 g         6860 t
ORIGIN      
        1 gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata aaaacaacat
       61 caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat tattatcaat
      121 tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat gactaatcag
      181 aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg ttttatcttc
      241 gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat catcggtgga
      301 tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact gaatgtggat
      361 ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt tgaaggagca
      421 atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac taataaaagt
      481 caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt attaaaagaa
      541 aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt ggtatttgtc
      601 gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc aacgatcgat
      661 gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga tgagatgtat
      721 gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac gagttccgaa
      781 actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc aactcaaaca
      841 gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct tgctgttcct
      901 ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag cttttatcga
      961 ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc tacaccagct
     1021 tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg cggtatccat
     1081 ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac gctcactaaa
     1141 ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc acaacaactt
     1201 cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt gcaatatttc
     1261 gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac tggttttgga
     1321 ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata tgatcctgag
     1381 acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa tcaaggcaaa
     1441 accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct gctagatatt
     1501 ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat ccatacaagt
     1561 atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt agggattgaa
     1621 cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa agagaatgaa
     1681 ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc tttagcagcc
     1741 gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat cgcagacatc
     1801 gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc tttaaaaatg
     1861 cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt gttgattgct
     1921 tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga aggaagcaca
     1981 atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct ttctgattct
     2041 tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt caaataagca
     2101 aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa taggtcttga
     2161 aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt cgaagaaagt
     2221 gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt gtagcaattt
     2281 tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg aagctcatac
     2341 cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt ggggtgacga
     2401 tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta cgtgatctat
     2461 tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg ctagtggctg
     2521 tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact gttgcacttg
     2581 ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga ccggtcattg
     2641 cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag aacaaatttt
     2701 gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata ctgacaacga
     2761 ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg gtttcttcta
     2821 gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat acaaaatgtg
     2881 tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc tgcgatgcag
     2941 gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga cttcctatag
     3001 aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac tagattacag
     3061 tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga agaattagtg
     3121 cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt tgaaggtctt
     3181 ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat caacttgcag
     3241 cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa atatatctta
     3301 cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg tcggaaccag
     3361 ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat aatcggtaca
     3421 ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag tttcttaata
     3481 gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg caaacggttc
     3541 tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt taaatagata
     3601 aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt tttgattttt
     3661 ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact gttaaggaaa
     3721 aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa tgaatgttga
     3781 attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat gccaaacatt
     3841 gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa ttcatctcaa
     3901 acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc aggtgctgac
     3961 aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga atcaaaaggt
     4021 ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa aatcgctaaa
     4081 taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa aacaaacgac
     4141 cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac tgccctgtgc
     4201 gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg atttgataat
     4261 ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat ttttttactt
     4321 cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca atatttgcct
     4381 gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat tgagaaagca
     4441 aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg tataaacgca
     4501 aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata ttatgttcta
     4561 acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga ttttgtccat
     4621 aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg gtcaacctca
     4681 agaataaatc aattgcactt cgttccattt tgattgagga attctctaag gtattcatca
     4741 gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt gctgtttttt
     4801 tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca aagaatacta
     4861 atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta ttttgttcga
     4921 ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat actaaccgtt
     4981 gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa cttgcatttt
     5041 ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt tttttgacaa
     5101 tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag taacaagatg
     5161 caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta ctcaccactt
     5221 tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt ttccactagc
     5281 ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg atccgttcgc
     5341 catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg gtgaccataa
     5401 cgattattta cgccgcagga tataatccca tgttttgctt caatatggga aagaaaatct
     5461 gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc tttcagatta
     5521 ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa aaataaaaag
     5581 cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc ctctcccttt
     5641 tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc atgtttgatt
     5701 tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa tggctcattt
     5761 cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga taacgtgggt
     5821 aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat tacacttatc
     5881 ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat cataccttgc
     5941 gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat cgtttctttt
     6001 cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa agcgatcgtt
     6061 gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc aacataaaac
     6121 aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc gaaaatatac
     6181 atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga agaaaaatcg
     6241 attattcgct caaataagat taatccctct tctatcagca aaagtaaagt ttccggcagc
     6301 cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt gaataccggt
     6361 gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg ccatacaatc
     6421 aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa ccaatctcca
     6481 tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc acccgcatga
     6541 aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa taagtccatg
     6601 gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag caaactagca
     6661 cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag tagactcaac
     6721 ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata cagccaccct
     6781 aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc actctcccta
     6841 aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa agaattaata
     6901 tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga tcctcttttt
     6961 ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt ttgaatttgg
     7021 aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt ataaagattc
     7081 cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc ctttcctcgg
     7141 aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag ctggagcttt
     7201 tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc acgaatcgta
     7261 tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga atactcttgt
     7321 agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc gataaaacgt
     7381 ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata accccaattg
     7441 tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat caaccgattt
     7501 cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt gaagttttga
     7561 aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt cgatcaacgc
     7621 ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca caccagcttg
     7681 caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt ctgaaccttc
     7741 cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat ggatcgttcg
     7801 caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat ctccactgac
     7861 tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg ctcctacttc
     7921 taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa agtactggtc
     7981 ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg tatcgcatgc
     8041 ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg attaaaaagt
     8101 actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct gacaaaagat
     8161 cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa aaattcttcg
     8221 aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat ggcgttttgc
     8281 tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat atttttgttt
     8341 tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat agtctttttt
     8401 cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag ttgtttatgc
     8461 tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata gctaagtaca
     8521 tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc cgtattcata
     8581 atatgaatcc cagatatttt attttggaga tagacattca gcattggttt ttttaatgtt
     8641 tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt cctgccaagc
     8701 tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg tgccagtcga
     8761 ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg tcttttcacc
     8821 gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt cttctctaaa
     8881 tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa gttctgatgg
     8941 agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct gttttccttc
     9001 cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag ggatatatag
     9061 gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg cttcttctaa
     9121 taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa aggaatagat
     9181 accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac gagcatcctc
     9241 ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg aagaaagtat
     9301 ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata ataaaaaacc
     9361 acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt tgggtgaagc
     9421 tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg accagtaaaa
     9481 atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc aaaatcgtcc
     9541 cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt gaagattttt
     9601 gacgaaaaag aggggttgtg acagaactcg tgtcacaacc cctctttaat tatttagttt
     9661 catttcattt tctctagata gtcaagcgct tcttgtactg tttttactgg tacgattttc
     9721 attgaggaat tcaatttttt agcggcagct tttgcttctt cgtagttggt ttttatttca
     9781 ggatattctt tcttaacatc gtctgaaatc gtatcatctg gtgcaaagaa aatctctacg
     9841 ccagctttat ccgcacttgc cactttttta tcgattcctc cgattcggcc aacttctcct
     9901 tcactgttga tcgttccagt tcctgcaatt tgcttaccat gtcgcaagtt tttaccagtg
     9961 acttgttcat agatttctaa agtgaacatc aatcccgctg atggcccacc gatagagcca
    10021 gaattaatac gaacatctgt atctgattcg atctcggtat ggtcagtaag cgtgatacct
    10081 attcctgcct ttttatctgt tggtaactcg atcagatcac cagaagctgt tttttcttca
    10141 ccattatgca aataactgat ttcgattgtt tgtccaactt tttggctttt tacataatcc
    10201 ataaatgctt ctgtactttg aaagcttttc ccgtctactt ttgttactgt atctccaact
    10261 gaaagcttgc caaagaaatt cgatgttttc tctacactca tgacatatac gcctttgaat
    10321 tccattttat aaggcttgtc tgcaagtttt aacgcttgtt cgattgctgc attttgggaa
    10381 gaatccatat aatactgttg catttgatta tattcttcat ttgtcgcccc accagtcaat
    10441 tcttcttcac tgatcaattc ttcaaaaggg gtcattttag cttttaaagc taagaaacct
    10501 gtagcttgcc tgataccaac agaggtaaga gagaaagacc cagaaccttt atcttcctta
    10561 tcattgaccg taattagttc tttcagatcg atcgtggctc ccggtgattc tacataatac
    10621 ggcaagggaa ctactacaaa tccaatgatc aacattccaa atatgataat cagtactgtt
    10681 ttcaaccatt gtttagcttt catttgattc acgctttcca gctaaagctt cgttgatcac
    10741 tggcggtaag tagctgctca catctccgcc aaagtgcagt acttctttta aaatactgga
    10801 actcacgtga ctatattctg gttttgctaa taagaagaca gtttccaatt catcatatag
    10861 atgatgattc atctgagcaa tctctcgttc atattcaaag tctttgatac ttcgtatgcc
    10921 tctgatcaac gcatctgcac caatctcttt tgctgtttct accgtcaatt gattttcttg
    10981 atgcatgact tttacattag gaagatgaga aacagctttt gtgatcaatg tcattctttc
    11041 ttctggggga aacaaacttt tctttgaagt attgataaaa acaccaatta ctacctcatc
    11101 gaataattta gccgcacgtt ccaccgtatc taagtgacct tttgtaaaag gatcaaaact
    11161 tcctgggaat aatgcacgtt tcattgattt ccctcttttc tatagatagt tacggccgtt
    11221 atgccataaa tctgttttct tatttgatgg aatccagcca cagtttctgg caattcgact
    11281 gttttatccg tttcgcatac gataactgcg gttttagaaa aaagtcccag ttcttccatt
    11341 ttatggattt gatcgattat tttttgtttt gcatatggcg gatcaagaaa caagtagtcg
    11401 aattgttgtt tctccgctgc tagccgttcg attgctttgt ccgcatcaag ttttattgtg
    11461 gtaaatttct ctggttcttt cgtaatttcg atgttttctt tgatgatttt tagcgcttgg
    11521 taatttttgt ctacgcatac cgcatgactg cagcctctag atactgcttc gatagctaaa
    11581 ccaccgcttc ctgcaaacag atccaatact gtctctccat caaaatatgg tccaatcata
    11641 ttgaatatgg attcttttac tttatctgta gttggtctgg tgttcgcccc tgctaaactt
    11701 tttaaacggc gcccgccata atttcctgat attatccgca tctactcact ccttagaaat
    11761 ggtactggta ctagtatacc aaaaaaatac ttggacaatg atcaaaaatc gatcgttatc
    11821 caagttattt tttatttaaa aactgctcat aagagatttt taaagatgtt cagctaatcg
    11881 gatttctgta tttagctctt caatttcgaa accttcgtct tttaatactg ttttgtttcc
    11941 tattttttcg gcaaaattca tttcgatatc cggtcggtaa gaacgttcta cgtttcgaac
    12001 aaaatgtaat ttacggattc gttcttctgt ttcttccaat gtctcttcat ttaagtaaag
    12061 cacgacatat ttcattcttc ttgagacata atgcaacaag ccgaactttt tcagattctt
    12121 taattgtttt aagctgtaga cccatacgat cagcccgcgt cgttttgtta tttcaaattc
    12181 actattgtct aattgcatga caattacctc cgcatccaaa gtgttttttc gtttcaaaaa
    12241 acgggctacc tgcatctact ttgatttcat tagaaacttc ttcggcaagt acttggacaa
    12301 tttgatccaa cagactttgc agctgtgttt cactatatcg gaaagctgca acatgctcat
    12361 ccatatcaag ctgtcttttt gcttttcgaa cagctcgttt tttttcacga taatcaggtg
    12421 catactcacc atatgccgct acattttcaa atgcttcttt cttagacaag aacgcttctt
    12481 tcaactggca aacttttgca gattcttcca ttgactgttt atgttcaaga taagaagcca
    12541 tcgttttact cttttttatc atttcagcga cttggaggca ttgatcctcc agttgaaaca
    12601 gctcttcgtt aacgatcact gctatcacta ctttctccgt tttcttcttg caataagact
    12661 aacatatttt ctttagaaaa agcaatgcct acagaatccg ttcccgggtc catgaaacgg
    12721 tcatggttgt aaggatcgct gtaccaatat aaaaacgaaa aagtagcgtc aatcgccgga
    12781 tgaataaata gcccactagt attttctaat ttttgtttac tgattaactt ctccaattca
    12841 tcattcgtca gtgagaattt cactgtcgtt tgagcactgc ctagcgattt ttgccaatca
    12901 gccatacgtt cctctgacaa tatttcctca ggccttgtca aaaaatcatg tagtagcagc
    12961 tgactgtggt tttgaaaggc aagtgtttga ttgtatatgg gaagttcgtc ttctcttcta
    13021 agcatattta ataaagtcgt actttttcgt tctttaacgc gattgattga ttcccaatca
    13081 gccgatttat ctgcttgata atgtggaagg ccttcaccaa acacttgata tggcaatagt
    13141 ttcaaaagac tatctttatc taaatacaca actgaaaata ggccgtttgt tgtttgatca
    13201 aaaaataaaa tagcaaaagt gtcgttatca aaagcaatta gcggacgata attcatatcc
    13261 tcttccatta gttcgattcc aacttctgta tcatggtact caaaagtgaa attagtatag
    13321 atagtagtga ttttcgtaag atcttgcatc gtcatcccta aataaaacgg cgcgatatca
    13381 gcaggccctg tttgcagtac tttaacagtt gtgactctgc tgtcttggat gttcgcttca
    13441 aatgtgagat gactagcttt attttcataa tatcttgttt caaaaccaaa tccgctagtt
    13501 aatcgatcat aggcttggcc aaaatccttt tccagctgat cgatcggttt tccgatataa
    13561 gtagaaaaac cagttgcttt aagctcctgg tatttccacg ttgtcatttg gtttgatatt
    13621 tgttgatcag agttttcctt cacttcaagt tttggcggaa acaagactgg ttccatgtag
    13681 aatgccacaa taactaatag cagaatcagc caaaacaaac ccagtctttt catgtttccc
    13741 cacctttcgc ttattatttc tcagtcactt caatcaaaag gtcaccagac gaaatagtgt
    13801 ctcctgaaga cacatataca tgtgcgactt ctccgtcaaa acgtgcttcg atggccgttt
    13861 ccattttcat cgcttcagta atcatcaacg tatcaccttt tttaacgtta tcgccttttt
    13921 tcactaatac atccaaaaca gaaccagaca tcgttgctcc aatttgttct cggtttgtcg
    13981 gttcagcttt caatcgggtt tggaccgtac tgatgatcga attatctttg atagtgattt
    14041 cacgtctttg tccattcaaa ttgaagaaca gtacgcgatt cccttcgata tcgggttctc
    14101 cgatttcatc taaacgaatg ataagcgttt ttccgcgttc gatttgtaca ttgattgttt
    14161 cacccaaacg tataccttgg aaaaatgttg gtgtgtccag taatgtaaca tcgccaaatt
    14221 gattatatgc tttttgatag tccaaaaaga cttgcggata catcaaatag cttaatactt
    14281 cgtcttgttt tggttcatag ccaatttttt ctgccaattc tttttttact tcgttgaagt
    14341 cgactgattc agccaaaaga cctggacgtt cattcaatgc aggtctgcct ttaagaataa
    14401 tcttttgcaa ttttttagga aatccgccaa cgggttgccc tagttctcct tggaagaaag
    14461 tcacaacaga ttcagggaaa cttagtgttt cgcctttttc ataaatatct tcttctgtaa
    14521 gatcattttg gaccataaat agtgccatat ctccaactac tttagatgat ggagttactt
    14581 taacgatatc gccaaacatc aagttgacag tatgatacat ttgtttgatt tcatcccagc
    14641 gatgacctaa tccgacagct tttgcctgtt gctgtagatt cgaatactgt cctcctggca
    14701 tttcatgcat atatacttct gtttcaggag cattcaagcc gttttcaaac ggtttgtaat
    14761 acatgcgcac atcttcccaa taatggttta atttctgtgc attttcaatc gtgatttctg
    14821 gcagccgtgg accattgacc agtgcataat acaagctgct catgctaggc tgactagtat
    14881 tcccactcat tgcactcatt gccacgtcaa cgatgtctac tcctgctttt gaagcagcag
    14941 aataagtgat gattccattc ccgctggtat catgagtatg cagatggatt ggcagatctg
    15001 tcgtttcttt caattcacta atcaaacgat aagctgcctg cggtttaagc agcccagcca
    15061 tatctttgat tgcaatgata tgcgcaccca gttgttctag ttcctttgcc atgtctttat
    15121 aatactgaac attgtatttt gcccgacttg gatcgttgat atcgccagta tagcaaatag
    15181 ctgcctctgc gattttacca gtatcccgta cagcttggat acttttttcc atttgtggag
    15241 tccagttcaa actatcaaag attcgaaata catcgattcc ttgcgctgct gcttctttga
    15301 caaattccac caatacattg tcaggataat tagaataccc aacggcattt gaacctctga
    15361 aaagcatttg cagcaacgta ttaggcatta aagaacgaat ttttctcaac cgttgccaag
    15421 gatcttcatt caaaaagcgg taagccacat caaatgttgc tcccccccac atctcacttg
    15481 aaaagagttc aggaagacct tctccagtca aacgtgcaat ctgcttgaaa tcctttgtcc
    15541 gaacacgagt tgctagcaga ctttgatggg cgtctctaaa tgtcgtatcc gtcaacaaca
    15601 aattttcctg tcctttgatc cattcgacca aagcatcggc acccttcgca tctaatacat
    15661 tcttagccgt tacgtaatca gttcttgtga ttagatcttt aggcatacgt ggttcttcat
    15721 aaaatggttt ttcgccactt tcgatacctg gaaaaccatt cacagtaatt tcaccgatat
    15781 atttcatcgt tttgtttccg cggtctcgta gtcgaggaaa ttcaaataat gttgctgtac
    15841 tatcaatgaa ggttgttttt gcatttcctg attggaattc aggatgtgtg atgacattga
    15901 gcataaatgg aatgtttgtt ttcactccac ggattctaaa ttctttcaag caacgttcca
    15961 ttttctgaat ggctgtttca aaggtagcac catgcgtaca tacttttact agcaatgaat
    16021 caaaatacgg tgtcactaca tagcccgcgt aagcatttcc gacatccaga cgtacgccaa
    16081 aaccaccagg tgaacgatac gtatcgattt tccctgtgtc tggtaaaaag ttattcaacg
    16141 ggtcttctgt agtgattcgg cattgaatgg ctgaaccatt taatttaatt ccttcttgct
    16201 gaggcaatcc gatttcttta tgcagatcca aaccttgagc aatcaacagt tgcgttgtta
    16261 cgatatcaat atctgtaatc atttctgtga tcgtatgttc cacttggacg cgagggttga
    16321 cttcgataaa gtaaaaatca tctccttcaa caagaaattc tactgtacct gcgttgacat
    16381 acccgacata tttcatcaat tggacagctg cctggcagat tttttgacgt tgctgttcat
    16441 tcatagagac acaaggcgct acttctacta ctttttggtg acggcgctga acagaacaat
    16501 ctctttcaaa aagatggatg acgtttccat gcgtatcccc gagaatctgt acttcgatat
    16561 gcttaggatt agcgatgtat ttttcaacat acacttcatc actaccaaag gctgcttttg
    16621 cttcgctttt cgcacgttcg tagccctctc ttgcactctt ttcatcgtga gctacacgca
    16681 tgccgcgtcc gccgccgcct agagctgcct tgatcataat cggataaccg tattgtttcg
    16741 caaactccaa ggcatcgtct atagaagcga ttggtccatc tgtgcctgga atcgaagcaa
    16801 tccccgcttc gatagctgca gcttttgctt tgattttatc tccaaaaata tctaaatgat
    16861 gcagtttggg tccaacaaaa atgatgcctt cttcttcgca ccgttgcgca aaattcaagt
    16921 tttccgacaa aagaccgtaa cccggatgaa tagcgtcagc tccgcattct ttcgcgatag
    16981 aaatgatccc ttcaatatct agatacgcat caatcggttt ttttccttgt cctactaaat
    17041 atgcttcatc cgctttgaaa cggtgtacgg aatattcatc ttcttctgca tagattccaa
    17101 cagttttaat tcctaattct gtacaagcac gaaatacacg tacagcgatt tcacccctgt
    17161 tagcaactaa aacttttttc atcgaaccac tcctacatct ttttttatta taaacctaag
    17221 ctcttgcgtt tttcatccgc actgatgttg aggacaaagc cgacacaaat cgaaagcatc
    17281 agcaaactgg agcctccttg acttaaaaat gggaacgtga tccctgtcaa aggaataacg
    17341 ccagtaattc ctccaagatt gacaaatact tggatcaaaa acattgcgcc gatcccaatg
    17401 cacaaaagcg aattgaacgg atcttttgaa cggataccta caaggacaat gcgcacaatc
    17461 ataaagaata gcaacgctaa tatcaaaatc cccataatca ctcccagttc ttccactacg
    17521 attgcataaa taaaatcggt ttgtgcttct tgtaaaaatc cttttttctg gatactgtta
    17581 cctaatcccc tgccaaatag tcctccgttg aacatggcat agtagccgtt gactagttga
    17641 tgccctttat ttaattcgtc tgagaaagga ttttggtaaa tagcaaatcg attatagata
    17701 tactgtaaac gtccgggtaa gatctttccg tttgtgatat tgatcagcca gatcgtaaaa
    17761 gtacttagac aaaagcctcc tactcctaca atcaaagtat acacataatt gactccgctg
    17821 gctaaaagca gaaccaaaat aatcaaaaag ataactgccg cattaccaaa atcgggaaga
    17881 atagcgacta atgcagtaag tccgataacc attgcaagtg gacgtttcac agtcccaaga
    17941 aaatcttttt gtacactgtt ttgtctttta gataaagtca gagacaggta ccagatagaa
    18001 atgattttta ggtattcggc aggttggatc gagaatcctg caatttgcag ccaacctttc
    18061 gccccattga tctccttacc aaaaaacaca acaatcagca gcaaaatcgt caaaactgcg
    18121 atggctgcca tgatcaaacg ctggtttttc aaaacatctg ttttcatttt ataaagtagt
    18181 gcgatagcaa tcaagctaag cacccaaaaa atactctggt taatgactga tgcggacgga
    18241 ttttgtccgt tttctaataa tagatacgac gtagaactat ataccatgat caatcccaat
    18301 acacataaaa ttaaataggg aatcaagatg ctatagtcta atagatgcct ttttttcact
    18361 ttcttaggca agtctattcc tccttgccaa cttctttttt atcataaatt tcttggtaaa
    18421 cctcactata taactgattc aattctcgtt ctagctcact taacatcagc tggccctcgt
    18481 tctttccgat tactcctata cggacagcaa aaccgacttg ccgggaaaag ccgtacatct
    18541 gtgtatctac cacttcttcg aaggctttgc attgtgagat acagagactg ttgttttgat
    18601 ttcggattaa tcgtttgatc cgttctgctt catcatttaa tacttggatc gcaagttttt
    18661 tcgacaaaga atccatctgt gcttacctcc cattctaatt ccttaattat aacatatttg
    18721 attttcataa ggagcaaaaa aaacagattt aaccagttgt ttttattcat ttttacgtat
    18781 tcgttctttc tacttatcca gaacgttaga gtccgcattt tttatccgag ataaaaaggc
    18841 ttcgccatat ttatcaagct tgctttggcc aactcctttt atctgaagca tctccaatct
    18901 gcttgatgga cgtattcggc tcatttcttt caatgtggaa tctgaaaata cgacatacgg
    18961 gggaacgcct gcttctgatg ctagatccgt tcgcagttcc cgcagcactt caaatagtgt
    19021 atcggtttct tcatccgata gctgctggac tttcttgggt tcctttcgat aaacagattc
    19081 ttgtcctttc aatacttgta tccctctttc tgttactttc aaaacaggat actcaccatt
    19141 gatggtcaga agatagccat tcgatatcaa atattcaatt aactgcatcg tttctttttg
    19201 cgattggttt ttcatcagtc cataggtaga taaatgtcca aaacctaaag cttgcagctt
    19261 ttgttctttc gatcctgcta agactttcat cagcatttgt ttaccatagt tctcacccat
    19321 acgttttaag caagaaagaa ccatctgtgc ttctgtcgtt acttctacta tttctctgtc
    19381 atctaaacag ttaccacaac ggttgcacgg actgcttgtc tcgccaaaat agtttaggat
    19441 gtattgctga agacaggact caatatacac gtattctgtc atcgctttta atttttcata
    19501 ttctttttgc ttttgccctt cttctctttg ggactgctgg ataaaaaact tttggacttg
    19561 gatatcttga ggagagaaaa gcaggatggc ttcactggaa agaccatctc ttcccgcccg
    19621 accagcttcc tgatagtatg cttctagaga gccaggaatt tgataatgga taacaaaacg
    19681 gacattgctt ttatttatac ccataccaaa tgcatttgtc gctacaatca gctgcaagcg
    19741 atcgtataaa aaggcttctt gcatttctgt tcgctcattc tcatttagac caccatgata
    19801 tctacccacg gaaaaatcga attttttaag caaatgatag agacggtcta cttcttttct
    19861 cgttgctgca taaatgatac cagattgatt tttgttgatc tttaaatatt cgattagata
    19921 ttgttctttt ttttgatctt taattacttg aaatcgtaga ttttctcgtt caaaaccagt
    19981 ctgaatatgg ttgttttctg gaatttttaa aagtcttttt atgtcagcag ctacttgcac
    20041 agtcgctgta gctgtcaaag caacaattac aggacgattt ggcagttgat ctagctggtt
    20101 cgccatctgt aaataacttg ggcggaaatc atgcccccac tgagagatac aatgtgcctc
    20161 gtcaatagcg atcatacgga cgttccatcg cttcattgct tggataaaat ccggcatgat
    20221 aaaacgttca ggagcgatat aaagtaaacg gtactgctgt ctatcgattt cttggaagcg
    20281 tctagctgtt tcgtaaccat ctaacgagct attgataaaa gtggcgggta tacctaattg
    20341 attcgccgcg tccacctgat ccttcatcaa agagattaaa ggggacacaa ccacagtaag
    20401 cccatctaat agtaaagctg gaagctggta acagattgat ttcccacttc cggtaggcat
    20461 gattcctaat acattttcct gatcaatgac tttctggata atttcttttt gaccaggtct
    20521 aaactcatca taaccaaagt attgttttaa taagtcgttc atcttcccgc tcctttctct
    20581 tgccatttta cttcgatttt cttcgaagga caagttgatc tcctttcgtg aaacattatc
    20641 ttattatgaa acaaaaactc tctagaaata agttttttaa aagtctttta aacgtctttc
    20701 ggcgtatttt ctgaatttat gtccat
//
LOCUS       DAZAOG010000031        20665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000031 DAZAOG010000000
VERSION     DAZAOG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.031"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="ACHDLD_001015"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="ACHDLD_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN3362767.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="ACHDLD_001016"
     CDS             complement(2019..2921)
                     /locus_tag="ACHDLD_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3362768.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="ACHDLD_001017"
     CDS             complement(2918..3610)
                     /locus_tag="ACHDLD_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN3362769.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="ACHDLD_001018"
     CDS             complement(3653..4294)
                     /locus_tag="ACHDLD_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN3362770.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4610)
                     /locus_tag="ACHDLD_001019"
     CDS             complement(4332..4610)
                     /locus_tag="ACHDLD_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362771.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4637..5263)
                     /gene="pyrE"
                     /locus_tag="ACHDLD_001020"
                     /pseudo
     CDS             complement(4637..5263)
                     /gene="pyrE"
                     /locus_tag="ACHDLD_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACHDLD_001021"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACHDLD_001021"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN3362772.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="ACHDLD_001022"
     CDS             complement(5983..6915)
                     /locus_tag="ACHDLD_001022"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN3362773.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="ACHDLD_001023"
     CDS             complement(6912..7688)
                     /locus_tag="ACHDLD_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN3362774.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACHDLD_001024"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACHDLD_001024"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN3362775.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /locus_tag="ACHDLD_001025"
     CDS             complement(10932..12008)
                     /locus_tag="ACHDLD_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN3362776.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="ACHDLD_001026"
     CDS             complement(12013..13293)
                     /locus_tag="ACHDLD_001026"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN3362777.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="ACHDLD_001027"
     CDS             complement(13312..14238)
                     /locus_tag="ACHDLD_001027"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN3362778.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="ACHDLD_001028"
     CDS             complement(14251..15534)
                     /locus_tag="ACHDLD_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN3362779.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACHDLD_001029"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACHDLD_001029"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN3362780.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="ACHDLD_001030"
     CDS             complement(16322..17233)
                     /locus_tag="ACHDLD_001030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3362781.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACHDLD_001031"
     CDS             complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACHDLD_001031"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN3362782.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="ACHDLD_001032"
     CDS             complement(17699..18397)
                     /locus_tag="ACHDLD_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN3362783.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="ACHDLD_001033"
     CDS             complement(18384..18770)
                     /locus_tag="ACHDLD_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362784.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="ACHDLD_001034"
     CDS             complement(18917..20587)
                     /locus_tag="ACHDLD_001034"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN3362785.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         5743 a         4684 c         3655 g         6583 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttcaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttatt
//
LOCUS       DAZAOG010000032        20324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000032 DAZAOG010000000
VERSION     DAZAOG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.032"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..705
                     /gene="purC"
                     /locus_tag="ACHDLD_001035"
     CDS             <1..705
                     /gene="purC"
                     /locus_tag="ACHDLD_001035"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN3362786.1"
                     /translation="EGKAKKIYLTSDENLIWIEYLDQATALNGARKDQISGKGELNNQ
                     ITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFSKRLGVPEG
                     KEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINEILTELFAK
                     VGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDLGDLIPVYQ
                     EVYNRLSELTEEKERV"
     gene            705..959
                     /gene="purS"
                     /locus_tag="ACHDLD_001036"
     CDS             705..959
                     /gene="purS"
                     /locus_tag="ACHDLD_001036"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN3362787.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            961..1632
                     /gene="purQ"
                     /locus_tag="ACHDLD_001037"
     CDS             961..1632
                     /gene="purQ"
                     /locus_tag="ACHDLD_001037"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN3362788.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1633..3855
                     /gene="purL"
                     /locus_tag="ACHDLD_001038"
     CDS             1633..3855
                     /gene="purL"
                     /locus_tag="ACHDLD_001038"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN3362789.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3840..5279
                     /gene="purF"
                     /locus_tag="ACHDLD_001039"
     CDS             3840..5279
                     /gene="purF"
                     /locus_tag="ACHDLD_001039"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN3362790.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5302..6354
                     /gene="purM"
                     /locus_tag="ACHDLD_001040"
     CDS             5302..6354
                     /gene="purM"
                     /locus_tag="ACHDLD_001040"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN3362791.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6351..6929
                     /gene="purN"
                     /locus_tag="ACHDLD_001041"
     CDS             6351..6929
                     /gene="purN"
                     /locus_tag="ACHDLD_001041"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN3362792.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6926..8461
                     /gene="purH"
                     /locus_tag="ACHDLD_001042"
     CDS             6926..8461
                     /gene="purH"
                     /locus_tag="ACHDLD_001042"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN3362793.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8477..9751
                     /gene="purD"
                     /locus_tag="ACHDLD_001043"
     CDS             8477..9751
                     /gene="purD"
                     /locus_tag="ACHDLD_001043"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN3362794.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9873..10787
                     /locus_tag="ACHDLD_001044"
     CDS             9873..10787
                     /locus_tag="ACHDLD_001044"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN3362795.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10920..12485)
                     /locus_tag="ACHDLD_001045"
     CDS             complement(10920..12485)
                     /locus_tag="ACHDLD_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362796.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12478..13929)
                     /locus_tag="ACHDLD_001046"
     CDS             complement(12478..13929)
                     /locus_tag="ACHDLD_001046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3362797.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13943..14959)
                     /locus_tag="ACHDLD_001047"
     CDS             complement(13943..14959)
                     /locus_tag="ACHDLD_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3362798.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15179..16267
                     /gene="chvE"
                     /locus_tag="ACHDLD_001048"
     CDS             15179..16267
                     /gene="chvE"
                     /locus_tag="ACHDLD_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3362799.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16321..17847
                     /gene="mmsA"
                     /locus_tag="ACHDLD_001049"
     CDS             16321..17847
                     /gene="mmsA"
                     /locus_tag="ACHDLD_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3362800.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17849..19054
                     /gene="mmsB"
                     /locus_tag="ACHDLD_001050"
     CDS             17849..19054
                     /gene="mmsB"
                     /locus_tag="ACHDLD_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN3362801.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19311..20288
                     /gene="guaC"
                     /locus_tag="ACHDLD_001051"
     CDS             19311..20288
                     /gene="guaC"
                     /locus_tag="ACHDLD_001051"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN3362802.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6501 a         3456 c         4662 g         5705 t
ORIGIN      
        1 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
       61 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
      121 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
      181 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct
      241 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
      301 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
      361 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaaacc ggaagaaatc
      421 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
      481 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
      541 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
      601 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
      661 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
      721 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
      781 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
      841 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
      901 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
      961 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
     1021 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
     1081 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
     1141 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
     1201 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
     1261 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
     1321 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
     1381 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
     1441 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
     1501 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
     1561 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
     1621 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
     1681 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
     1741 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
     1801 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
     1861 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
     1921 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
     1981 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
     2041 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
     2101 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
     2161 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
     2221 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
     2281 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
     2341 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
     2401 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
     2461 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
     2521 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
     2581 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
     2641 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
     2701 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
     2761 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
     2821 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
     2881 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
     2941 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
     3001 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
     3061 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
     3121 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
     3181 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
     3241 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
     3301 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
     3361 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
     3421 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
     3481 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
     3541 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
     3601 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
     3661 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
     3721 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
     3781 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
     3841 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
     3901 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
     3961 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
     4021 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
     4081 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
     4141 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
     4201 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
     4261 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
     4321 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
     4381 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
     4441 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
     4501 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
     4561 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
     4621 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
     4681 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
     4741 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
     4801 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
     4861 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
     4921 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
     4981 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
     5041 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
     5101 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
     5161 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
     5221 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
     5281 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
     5341 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
     5401 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
     5461 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
     5521 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
     5581 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
     5641 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
     5701 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
     5761 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
     5821 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
     5881 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
     5941 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
     6001 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
     6061 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
     6121 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
     6181 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg
     6241 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
     6301 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
     6361 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
     6421 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
     6481 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
     6541 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
     6601 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
     6661 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
     6721 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
     6781 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
     6841 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
     6901 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
     6961 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
     7021 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
     7081 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
     7141 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
     7201 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
     7261 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
     7321 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
     7381 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
     7441 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
     7501 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
     7561 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
     7621 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
     7681 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
     7741 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
     7801 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
     7861 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
     7921 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
     7981 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
     8041 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
     8101 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
     8161 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
     8221 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
     8281 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
     8341 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
     8401 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
     8461 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
     8521 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
     8581 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
     8641 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
     8701 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
     8761 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
     8821 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
     8881 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
     8941 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
     9001 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
     9061 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
     9121 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
     9181 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
     9241 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
     9301 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
     9361 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
     9421 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
     9481 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
     9541 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
     9601 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
     9661 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
     9721 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
     9781 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
     9841 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
     9901 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
     9961 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    10021 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    10081 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    10141 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    10201 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    10261 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    10321 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    10381 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    10441 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    10501 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    10561 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa
    10621 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    10681 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    10741 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    10801 caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg
    10861 agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat
    10921 tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt
    10981 tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata
    11041 tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct
    11101 gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag
    11161 ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt
    11221 tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt
    11281 gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg
    11341 tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt
    11401 ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat
    11461 cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg
    11521 tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga
    11581 agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg
    11641 gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg
    11701 acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac
    11761 gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa
    11821 aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa
    11881 aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct
    11941 gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc
    12001 aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc
    12061 aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt
    12121 actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca
    12181 tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat
    12241 atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg
    12301 aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt
    12361 gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca
    12421 gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta
    12481 tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt
    12541 tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag
    12601 gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt
    12661 ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat
    12721 aactttgaat tggatcatct catcaatttt gcatgcggta atacgaataa atcctttgtg
    12781 acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt
    12841 taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc
    12901 gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg
    12961 aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat
    13021 agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt
    13081 ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg
    13141 gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat
    13201 gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt
    13261 ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct
    13321 ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc
    13381 cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc
    13441 gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac
    13501 ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag
    13561 ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat
    13621 ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag
    13681 gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact
    13741 ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat
    13801 cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat
    13861 aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt
    13921 ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct
    13981 gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat
    14041 ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga
    14101 ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag
    14161 ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac
    14221 cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt
    14281 ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt
    14341 gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac
    14401 gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac
    14461 cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa
    14521 ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct
    14581 cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg
    14641 cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca
    14701 ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat
    14761 ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct
    14821 ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg
    14881 cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa
    14941 aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca
    15001 gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa
    15061 aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa
    15121 tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat
    15181 gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt
    15241 aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat
    15301 gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga
    15361 aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc
    15421 acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg
    15481 ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta
    15541 tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg
    15601 agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg
    15661 accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta
    15721 caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc
    15781 tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc
    15841 aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc
    15901 gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag
    15961 tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc
    16021 gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa
    16081 tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga
    16141 aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga
    16201 taaagataat tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa
    16261 aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt
    16321 atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg
    16381 ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac
    16441 ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat
    16501 gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa
    16561 gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa
    16621 gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca
    16681 gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta
    16741 gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa
    16801 tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg
    16861 aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca
    16921 cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag
    16981 acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg cgggatggtg
    17041 ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt
    17101 aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac
    17161 atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc
    17221 agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt
    17281 atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga
    17341 ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg
    17401 gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa
    17461 aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc
    17521 catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg
    17581 ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg
    17641 gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt
    17701 cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac
    17761 gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa
    17821 ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa
    17881 agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt
    17941 gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg
    18001 tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg
    18061 gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt
    18121 tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt
    18181 tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta
    18241 tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac
    18301 acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc
    18361 tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt
    18421 aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa
    18481 aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc
    18541 agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt
    18601 gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg
    18661 tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac
    18721 aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt
    18781 agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga
    18841 tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat
    18901 gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg
    18961 cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct
    19021 agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag
    19081 actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg
    19141 tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct
    19201 cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa
    19261 attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat
    19321 ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat
    19381 gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca
    19441 tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata
    19501 tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg
    19561 gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt
    19621 tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta
    19681 actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg
    19741 ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa
    19801 caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga
    19861 ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca
    19921 tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga
    19981 caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag
    20041 tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg
    20101 aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata
    20161 cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc
    20221 tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca
    20281 caatctaaaa aataaataaa atattaggat gttgtgaaaa agtt
//
LOCUS       DAZAOG010000033        19806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000033 DAZAOG010000000
VERSION     DAZAOG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.033"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..310
                     /locus_tag="ACHDLD_001052"
     CDS             <1..310
                     /locus_tag="ACHDLD_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3362803.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
     gene            783..1337
                     /locus_tag="ACHDLD_001053"
     CDS             783..1337
                     /locus_tag="ACHDLD_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3362804.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1510..1947)
                     /locus_tag="ACHDLD_001054"
     CDS             complement(1510..1947)
                     /locus_tag="ACHDLD_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3362805.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1995..2942
                     /locus_tag="ACHDLD_001055"
                     /pseudo
     CDS             1995..2942
                     /locus_tag="ACHDLD_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3213..3648
                     /locus_tag="ACHDLD_001056"
                     /pseudo
     CDS             3213..3648
                     /locus_tag="ACHDLD_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3664..4497
                     /locus_tag="ACHDLD_001057"
     CDS             3664..4497
                     /locus_tag="ACHDLD_001057"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362806.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4742..5767
                     /locus_tag="ACHDLD_001058"
     CDS             4742..5767
                     /locus_tag="ACHDLD_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3362807.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5905..6030
                     /locus_tag="ACHDLD_001059"
     CDS             5905..6030
                     /locus_tag="ACHDLD_001059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362808.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6055..7170
                     /locus_tag="ACHDLD_001060"
                     /pseudo
     CDS             6055..7170
                     /locus_tag="ACHDLD_001060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7253..8149
                     /locus_tag="ACHDLD_001061"
                     /pseudo
     CDS             7253..8149
                     /locus_tag="ACHDLD_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8165..8989
                     /locus_tag="ACHDLD_001062"
     CDS             8165..8989
                     /locus_tag="ACHDLD_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362809.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9021..10331
                     /locus_tag="ACHDLD_001063"
     CDS             9021..10331
                     /locus_tag="ACHDLD_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN3362810.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10479..11738
                     /locus_tag="ACHDLD_001064"
     CDS             10479..11738
                     /locus_tag="ACHDLD_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3362811.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11748..12620
                     /locus_tag="ACHDLD_001065"
     CDS             11748..12620
                     /locus_tag="ACHDLD_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362812.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12632..13462
                     /locus_tag="ACHDLD_001066"
     CDS             12632..13462
                     /locus_tag="ACHDLD_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362813.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13502..15685
                     /locus_tag="ACHDLD_001067"
     CDS             13502..15685
                     /locus_tag="ACHDLD_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3362814.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15746..16966
                     /locus_tag="ACHDLD_001068"
     CDS             15746..16966
                     /locus_tag="ACHDLD_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN3362815.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16963..18423
                     /gene="gtfA"
                     /locus_tag="ACHDLD_001069"
     CDS             16963..18423
                     /gene="gtfA"
                     /locus_tag="ACHDLD_001069"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN3362816.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18500..19606
                     /locus_tag="ACHDLD_001070"
                     /pseudo
     CDS             18500..19606
                     /locus_tag="ACHDLD_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6756 a         3006 c         3816 g         6228 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc
      361 cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga
      421 aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt
      481 cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta
      541 agaacttcta cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata
      601 agtaaggtta atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata
      661 aaatcataaa ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa
      721 tgattatggt aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa
      781 tagtgagcta taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt
      841 ggctaagaag aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata
      901 atgggttacg tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta
      961 agcctattat tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga
     1021 gagaagaaat tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc
     1081 ggcaacaagg tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag
     1141 aaaaattagg acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact
     1201 actacaagaa aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag
     1261 caattacaaa aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat
     1321 ttaaactagg tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta
     1381 tttttatcta gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt
     1441 acttggtata ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa
     1501 aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa
     1561 gaaaaatact gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc
     1621 gtttttgacc atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat
     1681 gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat
     1741 atcatccata gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga
     1801 cgcaccagat tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg
     1861 ttttaagtta ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag
     1921 gggcttattt tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct
     1981 catttttaga tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc
     2041 gaccgacatt tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta
     2101 atcaagacga tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa
     2161 ctgaactgac tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga
     2221 ttgaacgaac gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt
     2281 ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac
     2341 gttttgaagg gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata
     2401 ttgatgatta tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa
     2461 ttgatgccat tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg
     2521 taggctggta gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct
     2581 tagaaaatca agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc
     2641 cagttgccat tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat
     2701 ctgatttaca acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa
     2761 aagatcgtga attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa
     2821 ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg
     2881 aagaaattcc tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat
     2941 aattagctaa tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa
     3001 gaaaaacgca tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa
     3061 actatcattt tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt
     3121 tatacctctt tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt
     3181 ctcaactatt tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg
     3241 caagagaaac aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt
     3301 accagcgtat ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct
     3361 ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt
     3421 cgtccagagg ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag
     3481 attggtggca gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt
     3541 gtgttgtttt catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat
     3601 gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata
     3661 ggattgcttt atttttcggc tattgataat gctgattttg tattttctga tccatttacc
     3721 gcagaagttt tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg
     3781 cataatgtta attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg
     3841 ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt
     3901 cctgttgttt atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa
     3961 atgaatcgtt tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat
     4021 ttgattcatt atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa
     4081 ccaagtgtta ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta
     4141 acctttaaat cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta
     4201 gctgaattta ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt
     4261 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
     4321 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
     4381 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
     4441 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
     4501 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
     4561 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
     4621 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
     4681 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
     4741 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
     4801 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
     4861 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
     4921 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
     4981 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
     5041 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
     5101 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
     5161 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
     5221 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     5281 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     5341 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     5401 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     5461 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     5521 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     5581 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     5641 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     5701 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     5761 cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta
     5821 gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac
     5881 gcattcaaaa caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata
     5941 gctgttttaa ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac
     6001 gggcaagtaa ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa
     6061 atgttagatg aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct
     6121 tgggatcaat attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta
     6181 gtaaccatgc actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt
     6241 gatgatgtta tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat
     6301 ttctatacgc ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta
     6361 tggtacaaca aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca
     6421 tgggaaacac tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt
     6481 tttgcatcac atattggaac agagactggc tactggccat ttatttatca aaatggggga
     6541 gaagtatttg ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct
     6601 ttagcttttt atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta
     6661 caagatcctg ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga
     6721 aactggtttg caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc
     6781 gtgttaccta agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt
     6841 gcgagtacaa aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac
     6901 gccaataaaa tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac
     6961 gagtgggctg cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat
     7021 ggagtccctc gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt
     7081 attaaccgta ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa
     7141 gaagtcaatg cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa
     7201 ggattctatt tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa
     7261 tcgaattttt ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc
     7321 aacggcatta ggattaattt tattaaatat atggccagca attcaaacag caattttaag
     7381 ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa
     7441 aatgtttgca gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt
     7501 ttcgatacct agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt
     7561 taaaggattg tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc
     7621 agtcgcaatg atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt
     7681 ggctaaaata ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc
     7741 tattatcatt gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg
     7801 cttataagaa attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat
     7861 ccagcagttt ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt
     7921 aacaacagtt atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa
     7981 tagtaccgcc atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat
     8041 tttaaatgac aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat
     8101 tattactgca attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca
     8161 aattatgaaa aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg
     8221 tattgcaatg gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga
     8281 atctacccaa attccaccta caattttccc tgagaaattt cagctaagta actatcaaca
     8341 agtacttgat ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg
     8401 acgagtgatt ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag
     8461 ttttccaggg aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc
     8521 catttttatt actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc
     8581 agcattaata ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt
     8641 cttaggttta cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac
     8701 gatctttttc aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt
     8761 cacagcgttg tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga
     8821 taagatgcct ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc
     8881 acaattaatg gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt
     8941 ccaaagaaaa ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt
     9001 agtgattgga gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt
     9061 ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg
     9121 cattgtacga tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta
     9181 ataaaaattc gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt
     9241 taagagaggc tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag
     9301 tgattgattt tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg
     9361 ggattggtgg gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag
     9421 aaatggaaga agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt
     9481 taaccatggg tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc
     9541 aagtatgcgt accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg
     9601 actttcaatg gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa
     9661 atggggaaga tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc
     9721 cagattctgt acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg
     9781 aacacaatgc agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg
     9841 aattagggat tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg
     9901 aaagcatgcg agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat
     9961 atgcttcata tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg
    10021 tcttaaataa aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt
    10081 tagtcgataa aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg
    10141 ctttaaatcg gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag
    10201 ataaatcaaa aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt
    10261 taacagatat caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg
    10321 aatataatta aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag
    10381 ttaaatttag taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa
    10441 aagaaaacgc attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta
    10501 ttttagcttg tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg
    10561 aagatgcaag cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa
    10621 cgattcagga gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg
    10681 tggatgtacc gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag
    10741 atgtcattaa tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt
    10801 tagaggactt aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt
    10861 ttgctattga ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact
    10921 ataacaaaac tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg
    10981 aaaaaatagt agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag
    11041 aaggctggac tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag
    11101 aggaagcgaa caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag
    11161 aaatgcaaaa agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca
    11221 actggcaagg tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga
    11281 ttatgccgaa tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag
    11341 taggaacttt tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag
    11401 acctagcctt atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag
    11461 aatatatgac aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg
    11521 ctgttgaggg cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg
    11581 cttttactga tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt
    11641 ataccttaac aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa
    11701 atgctttctt taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag
    11761 aaattttaaa tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga
    11821 tttttttcta ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc
    11881 tgacttataa ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga
    11941 agtttatcaa atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa
    12001 ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct
    12061 ttagagcatg gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta
    12121 aacaaatttt taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac
    12181 aaaccagtct tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt
    12241 ggcaaggaat tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag
    12301 aaattattga agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg
    12361 aaatcaccta cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct
    12421 taacagcatt tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat
    12481 ctttggggtt acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg
    12541 ctattgcttt agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat
    12601 cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc
    12661 taaatacttg cttttattat tagggggctt catgatttta gttcccttag tttcaacgct
    12721 attcggttca tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc
    12781 agcgactttg gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa
    12841 ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc
    12901 ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt
    12961 aattttagga atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag
    13021 taaaattggt ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc
    13081 acagacacta tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga
    13141 ggctgctgaa attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat
    13201 gttaaaacca atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt
    13261 catgctacca ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca
    13321 atataattat acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt
    13381 aggaattgtc acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat
    13441 gagcaatgga gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact
    13501 tatgattata tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta
    13561 tctattagga atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac
    13621 gcactattct ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc
    13681 aaatgcgcaa gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg
    13741 acatggtgat tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa
    13801 tgattttcgt tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc
    13861 agccttatat acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc
    13921 gattagtgga ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat
    13981 tcgttctacg caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag
    14041 tcaatcaatt gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc
    14101 aagagaacgt caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa
    14161 acgcggatca agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga
    14221 tgaatttcaa ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt
    14281 gattcaaaaa gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt
    14341 tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag
    14401 taatgaaggt ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat
    14461 tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta
    14521 ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat
    14581 tgaaatgttt gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt
    14641 aggtgattgg gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca
    14701 agtgcatgat aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga
    14761 tagtgaacta tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc
    14821 aattagtcga aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta
    14881 tcaacaaatg acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa
    14941 tcgaaatatg acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc
    15001 tcatcgttat atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac
    15061 tattttattt gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta
    15121 catgccacaa acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata
    15181 tggaaccagc ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa
    15241 tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt
    15301 ttttggttat gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga
    15361 acaagttgcc ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt
    15421 ggaaagtccg tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga
    15481 agccatggtc ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa
    15541 attagcagga ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga
    15601 tgaattgatg aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca
    15661 acgcttttat ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa
    15721 gagactcatt ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc
    15781 tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg
    15841 gttagataaa ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact
    15901 agagtatggc atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat
    15961 tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc
    16021 acataatagt atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat
    16081 tggtgaagat ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc
    16141 tgaaactggt gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat
    16201 ttttacgaat caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca
    16261 cgatcaacca cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa
    16321 aaaaatgttg gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca
    16381 agaactagga atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac
    16441 gcattctcag tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc
    16501 tgtcaataaa cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa
    16561 tgccagattc tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact
    16621 caatgcagaa gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat
    16681 taaattaaga aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt
    16741 gaaagataaa aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt
    16801 agttatctta aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca
    16861 agtattaatc aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg
    16921 accgtttgaa agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa
    16981 gcaatgctca tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc
    17041 ttagacaaac atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg
    17101 acaggtgatc gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca
    17161 tgggaagatg tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat
    17221 cacatttctc gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca
    17281 tacaaagaaa tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa
    17341 gatattgatt taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg
    17401 gatggtacga cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg
    17461 accaaagaag ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc
    17521 tgttctttaa ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat
    17581 ttcttcgtgg aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa
    17641 tatgacgtcg aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc
    17701 aatcatgatt attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat
    17761 agtggaaaat cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca
    17821 acattggata cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa
    17881 gaattggatt acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat
    17941 tcaagtgcca attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca
    18001 ttaggcgata atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg
    18061 attccacaaa tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa
    18121 gcaacgaaag aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa
    18181 gaagtgcaac ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct
    18241 gcggcgtttg atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg
    18301 attacacgta aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg
    18361 aaagaattta tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt
    18421 taacctattc catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac
    18481 caataaaaag gactcataca tggataagta taaaacaaat acagcattta ataaatggtt
    18541 ttctgccata aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa
    18601 ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa
    18661 tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact
    18721 agaaaccgat gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga
    18781 acctaaagtg ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa
    18841 accaaccaag cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa
    18901 acgtcataag tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta
    18961 ttgttatatg gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga
    19021 acatgatctc aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga
    19081 tcgtgggtat taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt
    19141 cactcggatt aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga
    19201 aacagaaatt ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa
    19261 tttccgacta gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg
    19321 aatggacgtc tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt
    19381 gtttttcaag cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa
    19441 aggcgtgcag aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa
    19501 attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact
    19561 gtttcagccc tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt
    19621 ggctcgttac ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat
    19681 cctctgcgtc agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt
    19741 ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt
    19801 tttgag
//
LOCUS       DAZAOG010000034        19491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000034 DAZAOG010000000
VERSION     DAZAOG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.034"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..3648
                     /locus_tag="ACHDLD_001071"
     CDS             121..3648
                     /locus_tag="ACHDLD_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN3362817.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3645..7367
                     /gene="addA"
                     /locus_tag="ACHDLD_001072"
     CDS             3645..7367
                     /gene="addA"
                     /locus_tag="ACHDLD_001072"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN3362818.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7436..7777
                     /locus_tag="ACHDLD_001073"
     CDS             7436..7777
                     /locus_tag="ACHDLD_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN3362819.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8086..9129
                     /gene="pheS"
                     /locus_tag="ACHDLD_001074"
     CDS             8086..9129
                     /gene="pheS"
                     /locus_tag="ACHDLD_001074"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN3362820.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9134..11554
                     /gene="pheT"
                     /locus_tag="ACHDLD_001075"
     CDS             9134..11554
                     /gene="pheT"
                     /locus_tag="ACHDLD_001075"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN3362821.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11724..12734)
                     /gene="trpS"
                     /locus_tag="ACHDLD_001076"
     CDS             complement(11724..12734)
                     /gene="trpS"
                     /locus_tag="ACHDLD_001076"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN3362822.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12988..13725
                     /locus_tag="ACHDLD_001077"
     CDS             12988..13725
                     /locus_tag="ACHDLD_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3362823.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13739..14554
                     /locus_tag="ACHDLD_001078"
     CDS             13739..14554
                     /locus_tag="ACHDLD_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3362824.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14567..15214
                     /locus_tag="ACHDLD_001079"
     CDS             14567..15214
                     /locus_tag="ACHDLD_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3362825.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15230..15886
                     /locus_tag="ACHDLD_001080"
     CDS             15230..15886
                     /locus_tag="ACHDLD_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3362826.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16048..16869
                     /gene="racE"
                     /locus_tag="ACHDLD_001081"
     CDS             16048..16869
                     /gene="racE"
                     /locus_tag="ACHDLD_001081"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN3362827.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16872..18227
                     /gene="rph"
                     /locus_tag="ACHDLD_001082"
     CDS             16872..18227
                     /gene="rph"
                     /locus_tag="ACHDLD_001082"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN3362828.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18228..18746
                     /locus_tag="ACHDLD_001083"
     CDS             18228..18746
                     /locus_tag="ACHDLD_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3362829.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18853..19353
                     /gene="cbpB"
                     /locus_tag="ACHDLD_001084"
     CDS             18853..19353
                     /gene="cbpB"
                     /locus_tag="ACHDLD_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN3362830.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6558 a         3347 c         4183 g         5403 t
ORIGIN      
        1 cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat atagaacaaa
       61 tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag gaggaaaagc
      121 atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt cttgatcgac
      181 gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct tgttcccaac
      241 tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag aagcggaaaa
      301 aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc ttggtatttg
      361 ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc tgcaatgatc
      421 ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg agaaatcaat
      481 aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac aggaaatatt
      541 caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga ccagcaactt
      601 aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac tcaacgtgca
      661 ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca agatttcaac
      721 aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta tcaattactg
      781 caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg tccttataca
      841 gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta tttccgtctt
      901 tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc accagagcag
      961 actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc tagccaaaag
     1021 aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga agctgaaaat
     1081 ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag tgaagaaaac
     1141 taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg aaatatcctt
     1201 cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca aatgatggaa
     1261 aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta ttcttatcgg
     1321 ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga agcagaaacc
     1381 gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga catcactgaa
     1441 aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa agactggcag
     1501 tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt ggaagaacag
     1561 tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa aaaaataaag
     1621 aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat ccaaagtggc
     1681 gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa cttagaagcg
     1741 gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga atatgtagta
     1801 atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac aagtggtcta
     1861 gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat caatcgtttg
     1921 gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa tgatcaagtg
     1981 tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga agtattaaat
     2041 caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat ctcttttgag
     2101 cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct tagttatgca
     2161 caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag aatcacagaa
     2221 tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga ccctaattgt
     2281 tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg attagcttta
     2341 gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt actgaattcg
     2401 tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa tgttcctgtt
     2461 tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac ttcaatttca
     2521 cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg tttgaaattg
     2581 aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta tcatgaatca
     2641 ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa tatgtccgat
     2701 gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga acttcggttt
     2761 gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg acagacgatt
     2821 caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca tcctcttcag
     2881 acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct ggaattaccg
     2941 ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga tgtagcttct
     3001 gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg ttcattcgat
     3061 gtcacagaag cttattatgg catggctatg caattattga cgtatctgga cgtagcattg
     3121 atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc atattattta
     3181 catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac attgaaaaaa
     3241 tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact agatcattca
     3301 ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa agatgaatac
     3361 caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca actattcatg
     3421 gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg agaaatcaaa
     3481 ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc attcagaagc
     3541 atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga gaaaatagat
     3601 aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat caaattcctt
     3661 taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt gatcaaggag
     3721 ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt gttcgaagag
     3781 tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg acatttactg
     3841 aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa tccgtcaata
     3901 gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta ttgcccacag
     3961 cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat tattacttaa
     4021 ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc ttgatgaagg
     4081 aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa cgatttttcc
     4141 aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat ctggtgtttt
     4201 ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa cagttaagcg
     4261 ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa caaatccgtc
     4321 ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg actcagttag
     4381 cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa cagcagatca
     4441 agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct ggtttggaac
     4501 aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa atttccaatg
     4561 aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt aaaagttatt
     4621 ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg ttagtcgaag
     4681 aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa cgggaaaaag
     4741 gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca gaaaaaacga
     4801 aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc aaagaagtaa
     4861 tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac tggctaagag
     4921 aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa tccatctatt
     4981 ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc tcaaagaaag
     5041 aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa gtcctttctt
     5101 ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc aattatgacg
     5161 aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag tttgagccgg
     5221 aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg acggatgata
     5281 tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt cgtcaattaa
     5341 ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg atagagtaca
     5401 aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta gagattttca
     5461 aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa gcgacagaaa
     5521 ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat attccgcttg
     5581 cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc agcattcgat
     5641 tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca tcaaacgaag
     5701 atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg cgagaacttg
     5761 ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg ggatatctag
     5821 agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat gcattggttg
     5881 atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc gtgcgtttca
     5941 ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca atagaagata
     6001 atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca gttgtatttt
     6061 tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat gcttttgaag
     6121 aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta tatgatacgt
     6181 taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa gaaatgcgta
     6241 aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt tcctataaaa
     6301 ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag ttagtttttg
     6361 atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt tacggattga
     6421 tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct cttcttcaac
     6481 attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt gaacaaagac
     6541 agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa gatgaaactt
     6601 tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa gcttcttcac
     6661 aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat ccagatgata
     6721 cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc cgtcaatttc
     6781 gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg caagtgtcag
     6841 cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta gagatgccaa
     6901 ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta gtagacgaaa
     6961 aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca gaacttggtc
     7021 aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc atgctgcttc
     7081 ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta atccacggaa
     7141 tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt aaaacagatt
     7201 ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa tatcaaggac
     7261 agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta gaaaatgttt
     7321 tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta taattgagca
     7381 aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga gtatgatgga
     7441 gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct ctattttagg
     7501 aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc aacgctttgt
     7561 tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac gcttgaagga
     7621 attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga gccgttcaga
     7681 atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa tacaagtttg
     7741 gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa atcttgtaaa
     7801 ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg gctaaaccag
     7861 ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg aagttcactt
     7921 tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa gagtgcggtg
     7981 taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt aacaggaatg
     8041 aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc tctacaagca
     8101 caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca agatttaaat
     8161 gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac agaagtatta
     8221 cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt cgcaaatgag
     8281 atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga agaagaagcg
     8341 ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag aaaagtaaaa
     8401 catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat ttttgtgggt
     8461 atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa tttcgaacgg
     8521 atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta tatttcagac
     8581 gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga aaaacatgat
     8641 ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag agatacagat
     8701 gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga taaaaacatc
     8761 acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt tggggaagat
     8821 cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt agaagtagat
     8881 gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac tggatggatc
     8941 gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg aatcgatcca
     9001 gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat gttgcggtat
     9061 ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag tcagttcaag
     9121 ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag atttatcgaa
     9181 tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag tagagggagt
     9241 aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg aatgcgtacc
     9301 acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag acgaactgtc
     9361 tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg ttgcacttcc
     9421 taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg gccaagtatc
     9481 aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta taccgaaaga
     9541 atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc cagtttttcc
     9601 ttatttggat atggatgatt cgattattga attatctatc acaccgaatc gtgcggatgc
     9661 attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga ctcctacatt
     9721 cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc aagtggaagt
     9781 tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag taagaatcca
     9841 gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc caatcaacaa
     9901 tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac atgcatttga
     9961 ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa atgaaacatt
    10021 agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca ttactaacgg
    10081 caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga tcacgaatga
    10141 aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc gtcgtacagc
    10201 aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa tcaaccatgc
    10261 gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg cgggtgggga
    10321 agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat cagtgaagat
    10381 ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag aagtaaatga
    10441 gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt ccgtagtagt
    10501 accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag ttgctcgtat
    10561 ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg ctggaagctt
    10621 gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa gcggattgag
    10681 cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg ttataaatga
    10741 aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat tacgaatgaa
    10801 tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa atacagagat
    10861 tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta cacatctgcc
    10921 gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa aagattggca
    10981 gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa acttatttga
    11041 acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga aggaaatgca
    11101 tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg tcggtcaagt
    11161 gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg agtttgattt
    11221 atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga caaaattccc
    11281 agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc aggaattaac
    11341 agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat ttgatgtcta
    11401 tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga cattcgtcaa
    11461 cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag tcgaaaaagc
    11521 gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta agtaacgatc
    11581 aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc gtcacatctc
    11641 tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag gcctttacag
    11701 cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc tgattttact
    11761 tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg taagatttcc
    11821 ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg tgcaaattca
    11881 gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc gccgcggcgg
    11941 taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc caagtaggtg
    12001 aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt tggatcagta
    12061 tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata aataccgttt
    12121 cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc cattcctttt
    12181 ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt gaagctttgt
    12241 acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg tacaagattc
    12301 gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa tcctgtaggc
    12361 acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt gcgccgaacg
    12421 cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc cggaatttgt
    12481 gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata gtctaaagca
    12541 acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt cagtgcttgc
    12601 atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc cactcgggtt
    12661 tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc tcccgttaaa
    12721 ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac ttaatatctt
    12781 accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa gaggataaaa
    12841 taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc taagaaactt
    12901 tacaaattac taaactatca gatataatgt attttattat acatctttaa aagaaatctt
    12961 actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca cgtggataaa
    13021 tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa aggagaagtt
    13081 gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg tatcaatgga
    13141 ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca tgataaaaag
    13201 acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt caatttgtac
    13261 ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt gaagcaagac
    13321 caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat gttagataag
    13381 aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc aatcgccaga
    13441 ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc tttggatccg
    13501 gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg tatgtcaatg
    13561 attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt tatcttcatg
    13621 gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga aaatccaaat
    13681 gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga ggaaaaagat
    13741 gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc ttgcactagc
    13801 cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca aatctaccaa
    13861 tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg atattgagag
    13921 cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa agatccttgg
    13981 tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc cattattgaa
    14041 aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac gaaaaaaaca
    14101 ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa agaaaggaag
    14161 ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag tcaaaggttc
    14221 tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag aattagaaaa
    14281 ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga ctacggataa
    14341 tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg gtggaacgtt
    14401 tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct tgaaagctgt
    14461 taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct atcaaaaatg
    14521 gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga gcgaattgat
    14581 acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt cgagtgtctt
    14641 agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat tatcaaagaa
    14701 taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca ttccattatt
    14761 aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc tagatggatt
    14821 ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg aaacagtacg
    14881 ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt ctggttttac
    14941 ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg ttatcccgcc
    15001 attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa tcgtagctgg
    15061 tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata ccttcgatac
    15121 gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt acttaatggt
    15181 ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca tggattttag
    15241 cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg ttactgttga
    15301 agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag gtgtgattcg
    15361 ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata ttatacggaa
    15421 tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa tcggcattca
    15481 gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag ctatgttgtc
    15541 ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag caggattatc
    15601 aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag catttaaatc
    15661 aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt cattagcagt
    15721 aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac agaacaccaa
    15781 ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat gttacgcgct
    15841 ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga tgattctttt
    15901 agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga aaaaaagaga
    15961 tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag tacacaatag
    16021 ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg ccctatcgga
    16081 tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa acaattaccg
    16141 aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc tagacccgcg
    16201 gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca aggaatcaag
    16261 atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat caaagctgct
    16321 ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt aaaaaaaaca
    16381 caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca agcttatgaa
    16441 aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg tcccaaattt
    16501 gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat tgtggcagaa
    16561 acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg cacccattat
    16621 ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact gatcgattct
    16681 ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt gagcaattca
    16741 ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa acttttcgag
    16801 gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat cgaattggga
    16861 ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa ctggagcttc
    16921 aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt gatacaacag
    16981 ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa acaggaacag
    17041 gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga aatcgtagag
    17101 aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt attggtcgtt
    17161 ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc gtagactgcg
    17221 atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca tttgtggcat
    17281 tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat ccaataaaag
    17341 aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc acagatttgg
    17401 attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca gaatctggaa
    17461 aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa cagttgaatg
    17521 aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa aaacatgctt
    17581 tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca atcatcattg
    17641 cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa gcgggatatc
    17701 atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact ggcagcacat
    17761 ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat cagccagtct
    17821 tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga atctattctg
    17881 cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta ttatatgaat
    17941 tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta gtattcgcgg
    18001 cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta gcccgtatac
    18061 catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt aagaaacaaa
    18121 cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct caagcaatga
    18181 aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg cggtatttag
    18241 tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat cgttatcgtt
    18301 ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct gacccattat
    18361 gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc gaacagaaaa
    18421 aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta tcgaatgtcc
    18481 gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat atggtgtttt
    18541 ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt ttgaatcctg
    18601 gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt atcgaaagtt
    18661 tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt gaagagttgg
    18721 ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata aaaataagct
    18781 ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg aaatagaaat
    18841 ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa tcaggaaacg
    18901 tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt gtcccatggt
    18961 ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa agatgatcac
    19021 tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt gcaaacaatc
    19081 agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga tgttccgaca
    19141 attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc gcctttttta
    19201 ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga gctgcttaaa
    19261 gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc gaaaatcgaa
    19321 gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa gataatcaaa
    19381 aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga ggctgggaca
    19441 aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc a
//
LOCUS       DAZAOG010000035        19116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000035 DAZAOG010000000
VERSION     DAZAOG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.035"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHDLD_001085"
     CDS             complement(<1..703)
                     /locus_tag="ACHDLD_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362831.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHDLD_001086"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHDLD_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHDLD_001087"
     CDS             1758..2837
                     /locus_tag="ACHDLD_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3362832.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHDLD_001088"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHDLD_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHDLD_001089"
     CDS             complement(3572..3799)
                     /locus_tag="ACHDLD_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3362833.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHDLD_001090"
     CDS             complement(3902..6490)
                     /locus_tag="ACHDLD_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN3362834.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHDLD_001091"
     CDS             complement(6510..7169)
                     /locus_tag="ACHDLD_001091"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3362835.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHDLD_001092"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHDLD_001092"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN3362836.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHDLD_001093"
     CDS             complement(7942..8808)
                     /locus_tag="ACHDLD_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN3362837.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHDLD_001094"
     CDS             8917..9558
                     /locus_tag="ACHDLD_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN3362838.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHDLD_001095"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHDLD_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN3362839.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHDLD_001096"
     CDS             complement(10999..11898)
                     /locus_tag="ACHDLD_001096"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3362840.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHDLD_001097"
     CDS             complement(11900..12697)
                     /locus_tag="ACHDLD_001097"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN3362841.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHDLD_001098"
     CDS             complement(12675..13379)
                     /locus_tag="ACHDLD_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN3362842.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHDLD_001099"
     CDS             13510..14085
                     /locus_tag="ACHDLD_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN3362843.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHDLD_001100"
     CDS             14203..14880
                     /locus_tag="ACHDLD_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN3362844.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHDLD_001101"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHDLD_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN3362845.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHDLD_001102"
     CDS             complement(16943..17941)
                     /locus_tag="ACHDLD_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN3362846.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHDLD_001103"
     CDS             complement(18167..18820)
                     /locus_tag="ACHDLD_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN3362847.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5522 a         4136 c         3322 g         6136 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatct
//
LOCUS       DAZAOG010000036        18981 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000036 DAZAOG010000000
VERSION     DAZAOG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.036"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..973)
                     /locus_tag="ACHDLD_001104"
     CDS             complement(20..973)
                     /locus_tag="ACHDLD_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3362848.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(996..1751)
                     /locus_tag="ACHDLD_001105"
     CDS             complement(996..1751)
                     /locus_tag="ACHDLD_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362849.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(1748..2707)
                     /locus_tag="ACHDLD_001106"
     CDS             complement(1748..2707)
                     /locus_tag="ACHDLD_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3362850.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(2700..3650)
                     /locus_tag="ACHDLD_001107"
     CDS             complement(2700..3650)
                     /locus_tag="ACHDLD_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3362851.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            3800..4252
                     /locus_tag="ACHDLD_001108"
     CDS             3800..4252
                     /locus_tag="ACHDLD_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN3362852.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4341..6608)
                     /gene="gshAB"
                     /locus_tag="ACHDLD_001109"
     CDS             complement(4341..6608)
                     /gene="gshAB"
                     /locus_tag="ACHDLD_001109"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN3362853.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(6795..7793)
                     /gene="ftsY"
                     /locus_tag="ACHDLD_001110"
     CDS             complement(6795..7793)
                     /gene="ftsY"
                     /locus_tag="ACHDLD_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN3362854.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(7804..8613)
                     /locus_tag="ACHDLD_001111"
     CDS             complement(7804..8613)
                     /locus_tag="ACHDLD_001111"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3362855.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8622..12203)
                     /gene="smc"
                     /locus_tag="ACHDLD_001112"
     CDS             complement(8622..12203)
                     /gene="smc"
                     /locus_tag="ACHDLD_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN3362856.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12223..12909)
                     /gene="rnc"
                     /locus_tag="ACHDLD_001113"
     CDS             complement(12223..12909)
                     /gene="rnc"
                     /locus_tag="ACHDLD_001113"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN3362857.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13257..15038)
                     /locus_tag="ACHDLD_001114"
     CDS             complement(13257..15038)
                     /locus_tag="ACHDLD_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3362858.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15064..15978)
                     /locus_tag="ACHDLD_001115"
     CDS             complement(15064..15978)
                     /locus_tag="ACHDLD_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3362859.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16005..16967)
                     /gene="opp4B"
                     /locus_tag="ACHDLD_001116"
     CDS             complement(16005..16967)
                     /gene="opp4B"
                     /locus_tag="ACHDLD_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN3362860.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(16967..17911)
                     /locus_tag="ACHDLD_001117"
     CDS             complement(16967..17911)
                     /locus_tag="ACHDLD_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362861.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(17912..18916)
                     /locus_tag="ACHDLD_001118"
     CDS             complement(17912..18916)
                     /locus_tag="ACHDLD_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362862.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5197 a         4135 c         3366 g         6283 t
ORIGIN      
        1 taatcctctg ttatttatgc tatttaaatg cttgattcac atcttcgatc atcagtttca
       61 ttgattctaa accgcccccg cttaaatacc agacatctgg ttcaagggag atgatctgct
      121 gatttttacc agcattcgtt tgtgctacca gttcattggc agaaatatca tctttgctgt
      181 cgtctccacc aatagctttt gttctgtcta ctacaaataa gatatccgga tttttttcca
      241 gcacatattc ataagaaaca ctttgaccgt gagtggatgc ttcgatttga tcatctgctt
      301 gttcaaagcc aaataaatca tggatgaaac cgaatctaga accagatccg taggctgata
      361 attgcccttc attcacaagt gtgactagtg cttttttgtc agtagcttct gctttctttt
      421 tcgtctgatc gattgcacta gataattcct ctaattgagt tttagcttct tcttgcttac
      481 caaagatctc ccctaaagca gtgacatttt gctggaaaga ttcccatgga tttttcgcat
      541 ctaaagaaag gaaaatggtc ggagcaattt gacttagatc tttttgaaaa tctctttgtc
      601 ggccagaaat gatgattaga tctggctgaa gctgattaat tttttctaag tctggttctt
      661 ttattccacc tgctgattct acttttttat aggatgaaag atactcagga aggctgcttg
      721 tagctgctcc aactacctta tctccaacac caagagcatc catcgtatct aaagaaccat
      781 tgtcgaatac gacgactttt tctggattat ttggcacctc gatttggtct ccattactat
      841 ctgttatcgt caatgtagtt tcagtttgtg tgctgctttt ttttgtgtct gcctgactgg
      901 tgttgttgct atttccacat gctgctaaac caaatgatgc tagaatcaag atacctacta
      961 ctaatttttt catctcttct ctcctaatgt tagttttaga aataaagaca gaatctgcgg
     1021 ccttcaatct cacaaatccg tatgtccatt tcgtataatt catccaatac atcttttttt
     1081 atcatttctt gggtcgttcc ttgcgtaaat aactgtccat ctttcattgc aacaatttcg
     1141 tctgcataac ttgcagcaaa gttgatatca tgaagaacaa taacaattgt tttcccgatt
     1201 tcttgtacta aattttttaa aatctgcatg atttgaaccg caaaattcat atctagattg
     1261 ttcaaaggtt catccagtaa aatataatcc gtgtcttgcg ctagcaccat tgcaatatac
     1321 gcacgttgga gttgaccgcc tgaaagagtg tcaatcgatt gatccgctat ttcttctaac
     1381 ccaaggtgtt ccagtgattc ctgaacgatt tcctcgtctt ttgtcgtcaa ttttcctttc
     1441 gtataaggaa atcgtccaaa ttctactaat tctttcacgg tgatttggac atttatttca
     1501 ttcgattgtc gtaggatcga taattttttg gctaattcat tggatttcca agcctttact
     1561 tcgttatggt ctaaatagat ttcacctgta tcttttggta tcaaacgact gatcatcgac
     1621 aacaccgttg actttccagc accatttgga ccgataaaag cagtcaattt cttttcagga
     1681 atcgttaact gtacatcttt aaccactgat tttccatggt acagtttatt gaccttttgt
     1741 aaatcaatca taggatactc ttcctttcgc gaatcaattt tgtgacaaaa taaattcctc
     1801 cgacaaattc aacgacgaca ctcactgttg tattccagcc gaaaatctgc tctactgcga
     1861 gctgtcctcc tgtcagaaac agcatagcta ccagaaagct tccgataaat agctgaccat
     1921 gacgataagt agaaaagaca cgataactga tatttgcgac aataaaccct aaaaaagtaa
     1981 ctggacccac aagagccgtt gatgtccctg tcaaaagact gacacaacat aaaatcaaaa
     2041 attcaaaacg atctgtgtct atcccgaggt ttactgcttg ttcatttccg agatggaaaa
     2101 cgtctagcac atgtcgatat cgaaataaca ggtaccagca gccgataatg atcaaagaag
     2161 caggcaaaag atactgactc tggatattgc caaagcttgc aaataatctg ccttgaagca
     2221 aatcatattc attcggatcc atgatgactt gtaaaaaggt actgacacta ccaaaaagcg
     2281 tacctaaaat catccctgac atcagtaata aaaaaagatt cgctctcatt tttttcaaaa
     2341 gaaagaaaaa taacaataga ctcagcccga ccatcagccc aacgttcaat aagaaaaaaa
     2401 ctgtacctgt ctgctgaagc atcgtcactc ctccgataaa gaagaaaagg accgtttgga
     2461 caaggacata taacgactca agtcccaata ctgaaggagt caaaaactgg ttatgcgtaa
     2521 ttgtctgaaa gctgattgtg gcactgctga cagctagcga gacaaggata aatgcaaaaa
     2581 ttttcttact cctcaatgca catgcaaaat gccagtttcc atatgtatgg atcgtcaagt
     2641 acattcctgt aacaaataaa acaagaacaa gcataccaat aaaaatagat ggcttttttt
     2701 taagcatatc gtcgtcccct tatcagaaga taaaggaaga tagccccacc gatgattcca
     2761 acgatcacac tgactggtat ttcataagga aaaatcaaca cgcgagcgac aagatcgcaa
     2821 aatactaaga acaaactacc tgctaaagca gttacgccaa ttgttttgtg cacttgatct
     2881 ccatatattc tagagacaag attcggaatg atcactccga taaaaggaat gcttcctacc
     2941 atgatcaaca tgactgcact agccaatgat actaagccaa tgcctaaaaa ctgcatcttc
     3001 tggtaatcta ttcccagatt tactgcaaac gattcgccca gaccaatgat cgtaaatcgg
     3061 taagcaaata gccaaaccaa gagcaacaat ggaacaacag catacaataa ctcataattt
     3121 cctcgtatca cagtcgcaaa attcccttga agccatgaag tcatattttg taccaactgg
     3181 aattgataag caaaaaaagt agcaactgaa ccaatgatat tccctaacat gactccaatc
     3241 aacggaagca tcaattgatt tttctggagg aacaatcgcg taagggataa aaaaagcaat
     3301 gttcctgcaa aagcaaagca aaatgctgtc aaggatcgga caatcaagga tgcaccaggt
     3361 aggaaaatca tagccactaa aatacctaaa cgcgcactat ccatcgtgcc cgcagtcgtt
     3421 ggtgaaacaa atttattttg ggtcaaatgt tgcatgatca acccacaaat actactggtc
     3481 gctcccacaa ggatcaaaca aatcgttcgt ggcaaacgag tcactagcaa gatttgtaag
     3541 ctttgcccat ctaactggag cagttctgac aattgtacat tcgatactcc gatgaacaat
     3601 gaaatactac ttaaaataac taataatgct actagtcgtc cgtagcgcat ccaatccacc
     3661 tttctaattg agaatgattt tcattcttat tttaagtgaa tcgttgaaca aaagcaaccg
     3721 ctttttcata tgttttaggt attattggga atatgctata atcgtaagaa agacaaagag
     3781 aagaaggagg gtatttttta tggcacaaga tgatgtaaaa agccatttgg ataagggact
     3841 ttatggtaca cctttagtga accctgaaga acagcataaa tatttaggga cattcaggga
     3901 acgctgctat cttagtatga ctgttgctga aatgatccaa ccaaaaaaca aagaaaactt
     3961 tttaaaagaa ctcaaaaaac atccagatgc ttccatactc ttgaatgggg ctctacccct
     4021 ttctgtccaa aaccaatata tccagctagc aacgaaaaac aatagccgtt tcactgtagt
     4081 caacgattac gtcactgcaa atcctgagga attcggattg ctcttaacag caaaagaagc
     4141 agtaaatgaa cctgtgattg atatcgaaaa aaaataccca gaagaaaact cttcgccagc
     4201 tgaaaaagaa aaaccaaaaa agaaaaaaag cttctgggat cgtttatttt aaaacgaaaa
     4261 aacacataaa tttttacatg caaaaaaagc ttttgatcag attgacatca aataggacaa
     4321 tctgtcaaaa gctttttctt ttaaaatact tcaggatata acaatttcaa tacattcatc
     4381 gtcaatcttc tgccttttcc tgcaaaagga taaacatgca tatgcatggc tggattgaaa
     4441 tttgcttcga tgatcccata tgctttttta tcagttgtag gatcgatttc tttatctggt
     4501 ataatcaaat cgataccact aatcttagct cctagtgctt ctaccgcaga aacggcgatt
     4561 tttttgtaag cttcagaaaa ttcatcagtc atatcgatcg aatcaccacc tgtactgata
     4621 tttgagttct cccttagata aacaatttga ttggcttggg gaactgattc gatcgtcaac
     4681 ccttgttctt tcaacatcaa ttgttctaat tcacctaatt ggatcaattc taaaggtgca
     4741 cgatgattcg ttccacgcaa agggtcacta tttttttctt caacaagttc tttcactgtt
     4801 cggatactgt caccaataac attagcaggg actcgcaaca tgatggcttg gacttctccg
     4861 tcgatcacaa agaaacgata ttccgttccc ggcataaatt cttctactaa aaccgaactg
     4921 tcttcacgaa aagcaatggc taaacctgct tgataatctt ccagactagc tccttccttg
     4981 aaaatcgtga tacctaaccc gtaattggtt gatttaggtt tgatcacaaa tgcctgctcg
     5041 gcaaatctcg gataagcttt taccgcttct tccatggatg aaaattctgc gcctcctggt
     5101 acccggaacc cagcttcttt taagactttt ttcgttactg ttttgttttc catgataagt
     5161 gggacgatat agctgtcctt acttgtcatg tttgcatttt tcacatattc tacatggtct
     5221 tggaattgca gacgcaaaaa ttgatccgac tcatctaata ctttgacttg gacacctttt
     5281 tggatggcat cgaacataaa tatctgagta gaaagttcca tttcgcgaaa accggccaac
     5341 tgatagggac gttcatggct ttctttataa tattgatgtc ccaatgccac agcaacttct
     5401 tgctgactcg atagctgtgc ttcttgatag agtttcgctg ctaacgtttg ttcaggatga
     5461 tccatttgag tcaatgcttc ctctactaaa taaagagaat ccaaccaatc caattcaacc
     5521 agcatttgct tcattccttc aagaatttct ctaccttctt gatgaaattc tgtttgatct
     5581 gatggttgtt ctagtgctac tttgttattt cgagtcgtac cttctgctac ccattggtca
     5641 cagtcttctt tttcttctgt ccataacatg tacatcagga ataattgtaa gaaccgaact
     5701 tgctctggat tgatacccaa tcttgcataa ggattcaaat cgatattccg caattcaata
     5761 taacgaacac ctgttgttgc taaatctgcg actttctttc cgcctcggaa acgaagcggt
     5821 gtataaaatt ctttctcctc cgaaagtttc ccttcttcga tcattcgctc gatatctgct
     5881 aaatactgtt tcatagaagc ataggaaact ttcacatttg gatgattcgt atagcccagc
     5941 gcactgttgc gaatactgcg aacaggttct tgcggatgtg attcatctaa aaagtagttc
     6001 ttctcactca ttggtgaagc accgaataaa taagtgatca tccagcgata gcgcataaag
     6061 tttctcgctg ttttcaaata aagttctgtt ttgaattcgg aaaaatcttg aaattcttcc
     6121 tgatggctga acaatgttct tagcaactca tcgccaaatt caaaattgaa atgaataccg
     6181 cttaccatct gttttcgctt cccgtattct tttgctaaat aacgtcgata caagacatct
     6241 tcgaaatttt ttaattttgc aataatgatc tcttcgtctt tttccggtaa ggcaggtggc
     6301 atacttaatg gccagatcat ctcttctttc ggtatagaac gagcagtcac atcataaaca
     6361 gcagcgagat actgaaataa ttcgggaata gaatcggtaa caggggtgat catctctgtt
     6421 tgtgtttcgc taaaatctgt ttggatatag gggtgatagg atcgatcgcc aaagattgct
     6481 ggatgatcgg tttttgctag atttcctgca agatcaaccc gctgtccttc ccgttctata
     6541 ccaaatcgag cttggtcgat aaaagggcgc gcattgacat gcaataataa ttgcttaaaa
     6601 ttcatcatta agtgacctcc aattcatatg aatctaatta taatcaaaaa gtctaagaaa
     6661 aagaacgaaa ttgtttgaga accgcttaca aataagtata aaaatgaagg catcaaaaac
     6721 acagctatgt taaaaacaat cattcaaaca aggagctgca acttttgtca cagctcctgc
     6781 tttttagaca tgggttattc ttcttttagt aatcctttga acaatccaac gacaaaatca
     6841 tttggatcaa acggctccaa gtcatcgatt ccttcaccaa gaccgaccaa tttcactggt
     6901 aaatgcaatt cattacggat agcaagtacg ataccgccct ttgctgtacc atctaatttt
     6961 gtcaaaacaa gaccggtcac atctgttgtt tctttgaatt gttttgcttg aaccatcgcg
     7021 ttttgaccag tggttgcatc taatacaaga agtgtttctt ggggtgcttc tggtaattcc
     7081 cgctggatta ctcgttttat tttttctaat tcgttcatca agttcacttt attttgcagt
     7141 cggcctgcag tatcaactag taaaacatct gcctgctctg tctttgctct ttccaaagca
     7201 tcgaatacga cagctgctgg atcgcctcct gcattgccac gaacaacttc aacaccagcg
     7261 cgttcgcccc agacaactaa ctggtcgatt gctccagcgc ggaaggtatc tgctgctgcc
     7321 atcaatactt ttttaccttc gtttttgaac tgatgggcaa gtttaccaat acttgttgtt
     7381 ttccctacac cattaacacc gacaaacaaa ataactgtta gtccgtcttc ttggatattt
     7441 agttcattta cttcattgat tccttccgct tcatagagat ccacaagttt ctcgatgatc
     7501 gcattttgga cttctgccga ttttttcgca tttctgagtt tcacttcttg acgtaatgct
     7561 tcggtaattc gtatcgctgt ttcaaaacct acatctgcac cgataagtgt ttcttctaat
     7621 tcttcaaaaa agtcttcatc gactgttcga aaattggcaa acaactcatt catccgttca
     7681 ccaaatgttt tacgcgtctt ttctaaaccc ttgtcgtact tctcctgtac ttcaacttct
     7741 tctgttttct cttcgccagt aaaagcacgc ttgattttat caaaaaatcc catttgactc
     7801 cccctactct aatacaaatt tttggatcac tttggctacg ccattttctt cattgctggc
     7861 tgtcacgaaa tctgctgctt ctttgacgaa aggcacagca ttctccatcg ctacgcctag
     7921 tccagcatat tggatcatcg gtaaatcatt ttcctcatca ccgatcgcca tgatttcttc
     7981 ttgtttcaaa ccaagatctt ttgccaataa agaaatacca taagcttttg taacaccttt
     8041 tggcataaat tccaaaagca tacttcttgt tttgatgatc tcatacattt catggaattc
     8101 tgctggtatt tctttgattt taggatctaa ttcttcttgg tcataagcga ttactgcctt
     8161 attaagcaca aattcatctg taatatcgct tatatccgct ggttggaatt gcagcagctt
     8221 attcaacaca ttgtacaagg attgcttcct tggggcacta ggcagctgta aaaccacatt
     8281 gtcagacaaa acatctagtg gcagatccaa ttgctgagcc agtttgtata agcgttggat
     8341 atctgatact tccatcactt tcttttccat gatttcacca gtatcatttt tttgtaccaa
     8401 accgccgttg aaagtgatgc tgtagtcacc ttcatctacc agtcctaatt cttgcagata
     8461 atgcgccatc gcagctaagg ggcgtcctgt acatagaacg atcttgaccc cttgctgttt
     8521 tgcttgtgct aatgcttgtt tattctcatc tgaaatcagc ttttgttcat ttaataatgt
     8581 accgtccaaa tcgattgcta ctaatttgat cattttcgtt cctacacttt cgtttcgttg
     8641 attttcgcct cttcattgat atcttccatc cgtacagaaa cgatttttga tacaccggat
     8701 tcctgcatcg tcactccgta cagtacatct gctgcgacca tcgttccttt gcggtgtgtg
     8761 actacgataa attgtgtgtc atcttggaaa tcgctcaagt aacgaccaaa acgtttcacg
     8821 ttggcttcat ctaacgctgc ttctacctca tctaagatac aaaacggaac aggacagaca
     8881 cggataatgg aaaagagcaa cgcgatggct gtaagtgctc gttctcctcc agaaagcaaa
     8941 cttagacttt gcagtttttt gccaggaggt tgtacttcaa tctcaatacc tgttttcagc
     9001 aaatcagttg gttccgtcag tactaattca gctttaccgc cgccaaacat atttggaaag
     9061 acaattttga attcttgacg gatagcttca aatacttctt tgaatcttgt ccgcacttca
     9121 tcatccatct catccatcgt ttcaaacaat tggtttttcg ctgctaacag atcatctcgc
     9181 tgtgttgcta agaatgtatg tctttcactg acttgttcgt attgttcgat tgaatttaag
     9241 tttactggtc ccagtttttc aatctgttct tttaaggatg aaacctttgt tctactgtct
     9301 tcgatatcgg ttgtttcttg atagtcttgg gaagctttct caaatgtcag ctgatattct
     9361 gactgcagat aggtcaaatg attatctaaa atcatttcag ctcggtcttt ttggacttct
     9421 aatttggatt gttccgacag tcgtgctttt tgctcacgat tacgctctgc caagactgct
     9481 tcaagcttat cgatctcctt ttgccttcta tctcgctgct ctttggcttt tgccaactcg
     9541 gcttttaatg tttctcgctg agcagataat tcgttaattt gtttttccaa actttcttct
     9601 gtgacttcat gatcagaaaa gtctgctgtc aatgtcgcca actgcttttc tatcgcttct
     9661 tgacgttcgg ttgcctctgc tttttggact cgggcaccgc ggagctggat ctgcagatga
     9721 ttaaactgct ctttcaagac agcaagatct gcttgttctt gtgctttttg actttgtact
     9781 tgtgctcttc tggcttccat ctggtcgctt tcttgactta acgatttgat ttcttcgtcg
     9841 attttttgtc gttgcgattc aagatctgtt tgtttgtctt tcaattcgtt cgtttgcttt
     9901 tcgtaatctt cgataaactg ctgcagttca cgtgtctcaa aagttgagat ttgcttttct
     9961 ttttcaaaac gttcaagttc atttatgatg ttttgtagct gatttgtcgc ttcttgttct
    10021 tcaaaacgta attgttcgcc tcgtgtacgc aaaacttctt gttcttcggc taaacgagct
    10081 gtttcttgtt gcagctcctg aacttttttt tcttgtgctt ggagttgttt atctgcttct
    10141 tcaaattcac tagtcaactg tttcaattct tgattttgta caaacaaact gccttggttt
    10201 cctcgtttat tcgctccacc tgtcatggat cctccggcat tcatcacatc accttctaag
    10261 gaaaccactc gatattgata gcggatcgtt tgcgcgattg cattggcact agttaagtct
    10321 ttggctaaaa ggatcgttcc tagcaggttt tgaaccacag tttggatttg atcgggataa
    10381 ctgacttgtt cactggcaat ccctataaac ccttctacag ctgctgcttg tgtcaatata
    10441 tgagccggta attgtcttgg tttgattgtc gtcagtggaa ggaatgttgc tcttcctcct
    10501 ctttgctgtt tcaaatacgt gatggcttgc cgagcatctt tttcattttc aacgatgaca
    10561 tgctgggctg ccccacctaa agctgtttcg atagctaacg tgaaatctgc aggaacatcg
    10621 atcagttcag ccactgctcc gacaataccc gaaagctgct gtttatgctg caagaccaat
    10681 cgaacccctt ggtaaaaacc aaaatagttt tcttggattt cttgaaggct tttcttacga
    10741 gcacgtagct gctggacttg attcattaat tgatacattt taggctgttc ttttgctaat
    10801 gcttcttgga actttttccc gttttgttgg atcatttcta acttctcttt tgtttcagtt
    10861 aaagatgatt ttagctgcgc ctgcttttgt gtcaattctt ctttttgtaa gatcaatcgg
    10921 tctactgatg cttctacttc cgattgcttg gctaacgtct gcttgctttt tgctgtttct
    10981 tgtatatatt gacgttctag atacttcagt tcgtttccta cagctgcttg ttcttgcatc
    11041 aaatcaacat attgatctct taattctgcc aataattctt tcgttgattt ttggtatttt
    11101 tctaattctt gctgtgtttt tatgagattt gcttcagctt tttggatctc tgtttctttt
    11161 tctgctgctg ctttcatcaa gctttcttga agcttttcaa aatgtttaac ctttttttgt
    11221 gcttctgcta atgaagtttg atattcttgc gagctttttt gtgtatgctt tgtccgctcc
    11281 tgcaaaacat ctttttgtcc ttctgtctgc ttcagttttt ccgaaagatc taataatact
    11341 tgctgatttt tttcgatcag acgatcggct tgcgcatttt ctttacgctg cttcgccaaa
    11401 atggattctt gttcttggat cgactcagat agtttgccta attctagatt gaatttcgca
    11461 agctgtgctt gtttgttgtc ccaatctttt tttgctgtct tgatttcagc aaccatcaaa
    11521 gagacatcgg tttgagtcag tgtttctttt agccgtaaaa actcctttgc tgcctcactc
    11581 tgggcggcca aaggagttaa ctgttcttcc aattcgtgga tgatgtcttg gacgcggctg
    11641 aggttgtctt ctgtttcaaa aagcttctgt tctgctttct ttttccgctg cttgtatttc
    11701 aagacaccag ctgcttcttc aaagatccca cgacggtctt ccggtttact actgaaaatc
    11761 gcttctactt ttccttgaga aatgattgag aacgattctt ttcctaatcc agaatctaag
    11821 aacaattctt ggatatcttt taatctgcag ctttgtttat tgatgaaaaa ctcgctttcc
    11881 ccagtccgtc gataacggcg ggttacactg atctcattgt actccaatgg cagataatga
    11941 tcactattgt ctaaaatcac tgtgacttct gcaatattca gctgcttacg ggtatcggaa
    12001 ccggcaaaga tgatatccgg catttttccg cctcgcagac ttttagcaga ttgttctcct
    12061 aatacccagc ggatagcctc tgtaatattg ctttttccgc tgccatttgg tccaaccact
    12121 gctgtgacgc tattttcaaa atcaatgact gtcttgtctg caaatgattt aaaaccggct
    12181 atttcaattc gtttcaaata caaagctttc cctcacttac ttttacagtg ctgccaatgc
    12241 attttccgcc gcagcttgtt ctgctaattt tttcgatttc ccttgtccag tgccaatcaa
    12301 ttgatcatct acataaactt cgatccaaaa tactcgttca tgagctggac cttcttcatt
    12361 gatcaatcgg tattcgattg atacatcacc tgatttttgc aagacttctt gtagttttgt
    12421 tttatgatcc atctcatgtg aaaaagcacc tgctttgact ttaggaaaaa tcactttttc
    12481 gatgaatgca tgggtcgtgt cgaatccctg gtcaagatat aacgcgccta aaaaagcttc
    12541 gaataggtcg caaagcaatg ctggtcgcgt acgtcctcct gaattctctt ctcccttgcc
    12601 caacaaaaca tattgatcaa agtgacattc tttggcaaat ttcgacaagc tgtcttcgcg
    12661 aacgatcgca gctcgtgttt ttgtcaattt tccttctggc aaatgaggat ataaacggaa
    12721 taaatactga gaaaccatca gttctaaaac ggcatctcct aaaaattcaa gtcgctcatt
    12781 atccgataat tgcagattgc gatgctcatt cacataggat gaatgggtaa aagcttgttc
    12841 aagcaaattt agatcgtgaa aaacgatgtt atagttttct tttagttctt ttgtcagctg
    12901 gttgtccatt ttccacattc cttttatgga tattttttcg atacttcctt attatactgg
    12961 ttttttgtca aatttgaaat ggttcggtca aaaggtaagt aaagctttaa taatcctagt
    13021 gattgacgag aagttgtgaa aaattcgttt agtttcgagt atgaagaaaa aattttgtga
    13081 aaatggcttt ccatattttt gcataaattt gacttaatac cgagaaactg gactttgaac
    13141 accgtttatt ggagaagtca tgaaatcagc gccttgacct gaatattaga gaaataaaca
    13201 gaaaaatatc aaaaaaccga aaaaggacat cgcccttttt cggtcatttt tagtgattat
    13261 gaacgtgaat ctgctgtaac agaaacattt gcccatgggt ttggtacatc ataagaccaa
    13321 tcccagtctt tgacacggtc gttgactggc aatacttcat tacggaacaa cgttggaatc
    13381 gcaaatgctt cttcaaatgc atattgttgc catttttgat atgcttcttt ttggtactct
    13441 tcatcaaatg ctttgcttga actgatatcg ttgatcaatt ttgtattttc ttctgattca
    13501 taacgagtgt agttgaacgc agcatttggt ccccatagtc cacttggatt tggatcagtt
    13561 cctgtattcc aagcagcttg gtaaacatca atttcaggat catcgttttc taatttatca
    13621 tagaaagaat tgaattcgat cagacgacct gttgtgtatt ggacatccaa accaatttct
    13681 ttccattgtt ggatatagta atctgccaat ggttgcgctg tttccccacc ggacatcgaa
    13741 gcaaagttga ttgttagttt atttccatct ttgtcttctc ttaaaccatc gtcatccaca
    13801 tctttgtagc cagcatcatc taatagtttt ttcgctttgt ctagatcttg tgtgaatcct
    13861 ttcatctcat catcatggta tgactggaat actggaggaa tcaatgaagt cgcattgctt
    13921 ctcaaacctg catagaaacg ttcacctact gcattattat ctaatgcata gcccattgct
    13981 tgacgtaatg atttatcagc catttttgaa tctggattgt attccacact ctttgtatct
    14041 gcattccatt tacctaactt gaatcctaga taagtataag aagtttgttc acggcctaag
    14101 tttgtatagc cagcaatttc tttataggta tcgtaagtat ctgttggcat ttggaaaatc
    14161 atatcatata atttagagtt caatgcttca gttgcacttg ccatcggtac agatttcatc
    14221 gtgatctttt tcaatttagg cgtaccattc cagtagtgct cgtttggcaa gtattctact
    14281 gattctcctg caacgatatt actcatgtaa taagggccaa aagtcactgg atttttacgt
    14341 acttgatcgc ttgattccat atctttgatt gctacacctt ctaacgtatg ttttggtgct
    14401 gcatatgacc agataccacc acccgattga agcatgcttg ggtttacttc tttatattcg
    14461 atttcaactg ttttgtcgtc tatttttttg attccggaga ttgtgtcggc acttccatca
    14521 tgatattctt ccatacctac gatgttacgg aaagtgtcgt cataacggat accagtgtaa
    14581 tctttattcc caatgatttc gtaagggaaa atcacatcgt ccgccgttac atcttcacca
    14641 tctgaccatt tgacgttgtc ttttactttg attgtcgctt ttttcgtatc ttcatctaga
    14701 tcaagcgttg cagctccgtc gtctgtgatt ctgaaatcgt catcgtttgc aaatagcggt
    14761 tcatgagaag gagccataaa agctgcatca tatgaatctt ggtaaaactc ccactggaac
    14821 aatcctttaa actgactgtc cgttgcgacc gcagcttcta attcgccgtc ttcgattgca
    14881 tcatcattat tttttgttgc aattggaaaa gttaattcct ctttgttatt gccgttgcct
    14941 ccagagttgt ttccagaggt atctgtacct ccgcccccgc atgctgcaag aacgacagca
    15001 gacatcagcg ctgcaaatcc aaaaatcgtt ttcttcattc ctatttcccc cttcgcttct
    15061 atactagcct aaccgttggc gcgcatctgc agaacgcttg aatgcttgtc caacataatt
    15121 gatactcaac atcaaaacta aaataaggat tgatgcgggt aaccagatcc attgccgatt
    15181 cactaagact tctccgttac ttgcataacc gatcaatgta cctaaactag gtacatttgc
    15241 cggcaagccg aaacctaaga aagtcaaagt tgtctcgata ccgatgtttg ccgcaaagtt
    15301 catcgtcaaa ttagtaatga ttaatgaact tagattcggc atgatttcgc ggaacatgat
    15361 tttaaaatca ttggttccca ttgtttttga ggcactgaca taatctcttc ttgcttcaga
    15421 taatgtcttg cttcggaata atcgggcttt agctacccaa taaaatgcac tcatgatgaa
    15481 tataaaggac cagacattgt atctggaaat aatcgatacg aagacgataa tgatcagcat
    15541 gatcggtaga atcatgataa aatcaacgat ccgcatcaag atgttgtcaa tgatccctcc
    15601 gtagtagcca gagatgatcc ctaatccaac cccgatgatc gaagtaagga tcgtgatcgc
    15661 aaatccgatc gtaacagagt ttctagcacc tatgatcaat tgtcctaata catcacgtcc
    15721 gccttcatct gctcccagaa tgaacttttg tccttgtgtc gaaactgttc ctggtgcagc
    15781 gtatttgtcg aagatactaa catacatcac tttgtcttga tcggtcagca tcgcaccgat
    15841 aaagatcact aataataaaa ccactagcaa acctaatgaa aaaatagcaa ttttatcttt
    15901 tttaaattct cgagcaatca ttcgtatccc cataggagga atactttctt gaactgctgt
    15961 attgtttttt tcactcatcg atccattctc ctttctgatt ggttttattg aatccgaata
    16021 cgcgggtcaa cgatactcat gatgatatct gaaagtaatg tccccactag cgtcgcgatt
    16081 cctaaaatca atactaagga tgtgatcacg gtatagtccc gttggccgat ggaatcaata
    16141 aatagttttc cgactcccgg ataaccaaaa atcttttcga taactacaga accagcaatc
    16201 aacgaagtga tttcataccc catttgagaa gccatcggca acgctgcatt tcggaaaata
    16261 tgtcgactga atactttatt cgtcggtacc cctttggaac gggctgttct gacaaaatcg
    16321 agcgatttcg aatcgattac ttcacttcgc agatattgga ttgtgacggc agtcgccaaa
    16381 aatgcttggg taattgatgg taacagaata tatttcaaac ggtctatcca ttgagtgata
    16441 aatccacctt gcaaacctag tgtttgtgat ccacttgttg ggaaccagcc taaacgataa
    16501 ccaaaaacaa atagcatgat caagccgaag ataaataaag gaacagcaaa actaaagaag
    16561 ttgtagatta cgacaatctt atccaagatt gaattttgat accggccagc aagcatcccc
    16621 agaggcacag caatcaaata agtaaggaca actgtcaaga tcgatagata taaagtgttg
    16681 atgatccgtt cgccgatcaa gtcagcaaca gagcgtttgt ataagaaact ttgaccaaaa
    16741 tcgccttgca aagcatttcc gatccaacgg aagtactgga cataccatgg gtcattcaag
    16801 ccagctgctt gtcgcatctg ttcgatcact gccggatcct gatttggatt gatcaaaccg
    16861 gtaaatggat cccccggcat cgcttgggct aataagaaga tcagaacact taaaataata
    16921 acttgcggga tcatcaataa aacacgtcgc agaatcgttt tccacattag cttgcacctc
    16981 catctttcaa cgccactttg tgtgtagttg tgatcgtacg caaatcgtag actcgaccag
    17041 tcgcatcata gtattctttt tggttttgga tatactcttg ttctactctt cgtcgatttt
    17101 ctttgtggct ttccctattt tctacatcga tttgcggaat agctgaaagc agacgtttcg
    17161 tatagatatg tcgcgggtca ttataaatgt cttctcttgt accgatttct acaaatcgac
    17221 ctttgtacat aattgcgata ttgtcgcaca tgtgttttac tacacctaaa tcatgtgaaa
    17281 tgaataaata gctgaggccg tattcttgct ggattcgttt catgaaattc aatacttgcg
    17341 cttgaacaga tagatccaga gcagatacag gttcatcggc tacgatcagt tttggattgg
    17401 tagcaacagc acgggctact cctagacgtt gacgttgtcc gcctgaaaat tcatgaggat
    17461 atttatatag cgcatcttcc ggcatcccaa cgatatctag cagtcctttt acctttttct
    17521 tttcttcttg atcgcttaac cgttcaaaat ttcggatggg ttcagcaatg atatccaaca
    17581 ctcgcttttt aggattcaaa ctggacattg aatcttggaa gatcatctgc acatccttgt
    17641 tataacccat ttttttgcgg ttgcttcgct tcgttacatc ttttccttca tacatgattt
    17701 gtccggctgt taccttttct aatcccacaa cggcttttcc cgtagtagat tttccagagc
    17761 cagactctcc cactaaacca tatgtctttc ctttttcgat aataaaatcc acaccatcta
    17821 cagcatatac atgatccgtt actcgattga agaatccact gcgaataggg taatgaactt
    17881 tcaggtcttt gattgtgatt aattctgtca tctacacctc tcctttttcg tctctaaagt
    17941 ggaaatgctt atagcaggag caacgtacca agtggttcgg tgcgacttca tgaaggatag
    18001 gctcttcttc atgatcgctt gctggtatcc atggaatacg tggtgcaaac cgacaacctt
    18061 tacgaggcat gtttttcaga gaaggaacga caccttcgat cacatggagt tctgcatcat
    18121 gatttccttc ttgtggtata gagttcagta aagagcgtgt ataaggatgc ttaggatgat
    18181 caaacagttc ttttacacct gctacttcaa cgaactgtcc gccatacatc actgccactc
    18241 gatcagccat ttctgctacg actcctaagt catgagtgat caagatgatc cctgtttttg
    18301 tttcttcctg aaggtctttc agtaaatcaa gaatctgtgc ttggatggtt acatctaggg
    18361 ctgtcgtcgg ttcatctgca atgacgattg gaggtttgca ggcgattgcg attgcgatga
    18421 tgacacgttg tctcataccg cctgatagtt catggggata ttggcgtcca actctcgcag
    18481 gattaggaat ccctacttgt tctagcaatt ctagtgcacg agcttgacgc tgttctgcat
    18541 tcatctttgt gtgataggaa agtccttctt tgatctggtc ttcgattctc atgagaggat
    18601 ttaatgcaga caacggatct tggaaaatca ttccgatatc atttccgcgg attttattat
    18661 agagcgtttc gttcaggtcg attaaattca gatcgttata taaaatctca cctgtaattt
    18721 ttgtgttatt aggatcaagt aatcccataa tcgtagtagc caaggtactt ttcccgcacc
    18781 cagattcccc aacgattgct aaaatctcgt ttttgtctaa agtaaatgaa acgtcatcta
    18841 ctgcatcgta atagtcatct ttgagtcgga atcctgtatg taggttctga acttctaata
    18901 attggttatc ggacaatcga atacccctct ctgcttccta aatggaagtt tttcttaata
    18961 aaaaaggaca ttacacagaa t
//
LOCUS       DAZAOG010000037        18973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000037 DAZAOG010000000
VERSION     DAZAOG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.037"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1546
                     /locus_tag="ACHDLD_001119"
     CDS             119..1546
                     /locus_tag="ACHDLD_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362863.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1638..2003)
                     /locus_tag="ACHDLD_001120"
     CDS             complement(1638..2003)
                     /locus_tag="ACHDLD_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN3362864.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2000..2365)
                     /locus_tag="ACHDLD_001121"
     CDS             complement(2000..2365)
                     /locus_tag="ACHDLD_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN3362865.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2557..2805)
                     /locus_tag="ACHDLD_001122"
     CDS             complement(2557..2805)
                     /locus_tag="ACHDLD_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362866.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2804..2932
                     /locus_tag="ACHDLD_001123"
     CDS             2804..2932
                     /locus_tag="ACHDLD_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN3362867.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2989..4683
                     /locus_tag="ACHDLD_001124"
     CDS             2989..4683
                     /locus_tag="ACHDLD_001124"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN3362868.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4783..4956
                     /locus_tag="ACHDLD_001125"
     CDS             4783..4956
                     /locus_tag="ACHDLD_001125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362869.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5334..6467
                     /locus_tag="ACHDLD_001126"
     CDS             5334..6467
                     /locus_tag="ACHDLD_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN3362870.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6498..6818
                     /locus_tag="ACHDLD_001127"
     CDS             6498..6818
                     /locus_tag="ACHDLD_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN3362871.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6847..7311)
                     /locus_tag="ACHDLD_001128"
     CDS             complement(6847..7311)
                     /locus_tag="ACHDLD_001128"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3362872.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7443..8696)
                     /locus_tag="ACHDLD_001129"
     CDS             complement(7443..8696)
                     /locus_tag="ACHDLD_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3362873.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8725..9042)
                     /locus_tag="ACHDLD_001130"
     CDS             complement(8725..9042)
                     /locus_tag="ACHDLD_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3362874.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9068..9388)
                     /locus_tag="ACHDLD_001131"
     CDS             complement(9068..9388)
                     /locus_tag="ACHDLD_001131"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3362875.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9852..11267)
                     /locus_tag="ACHDLD_001132"
     CDS             complement(9852..11267)
                     /locus_tag="ACHDLD_001132"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3362876.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11284..12600)
                     /locus_tag="ACHDLD_001133"
     CDS             complement(11284..12600)
                     /locus_tag="ACHDLD_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3362877.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12750..13448)
                     /locus_tag="ACHDLD_001134"
     CDS             complement(12750..13448)
                     /locus_tag="ACHDLD_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN3362878.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13737..14726)
                     /locus_tag="ACHDLD_001135"
     CDS             complement(13737..14726)
                     /locus_tag="ACHDLD_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3362879.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14806..15639)
                     /gene="msrA"
                     /locus_tag="ACHDLD_001136"
     CDS             complement(14806..15639)
                     /gene="msrA"
                     /locus_tag="ACHDLD_001136"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3362880.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15814..17448)
                     /locus_tag="ACHDLD_001137"
     CDS             complement(15814..17448)
                     /locus_tag="ACHDLD_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN3362881.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17435..18961)
                     /locus_tag="ACHDLD_001138"
     CDS             complement(17435..18961)
                     /locus_tag="ACHDLD_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362882.1"
                     /translation="MNYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIW
                     LLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPA
                     VLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKND
                     IKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQAL
                     NNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTP
                     ISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENY
                     AKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKG
                     KYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGE
                     NKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK
                     "
BASE COUNT         6018 a         3728 c         3163 g         6064 t
ORIGIN      
        1 aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag
       61 aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat
      121 gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta
      181 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
      241 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      301 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      361 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      421 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      481 attaaaaaaa caagtattag cttactttaa tgactgcggg atcgaaatcg accgctactc
      541 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      601 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      661 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      721 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      781 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      841 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
      901 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
      961 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
     1021 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
     1081 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
     1141 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1201 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1261 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1321 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1381 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1441 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1501 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1561 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1621 acgtcaggct cacaacctca cttattttgg acatctaggg gtccgatctc tttatttaaa
     1681 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1741 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1801 atatagcctc ccaacatctt tttaatagca ttgttctcgc ttactcggtc gctttgcact
     1861 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     1921 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     1981 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     2041 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     2101 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     2161 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2221 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2281 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2341 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2401 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2461 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2521 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2581 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2641 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2701 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2761 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2821 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2881 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     2941 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     3001 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3061 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3121 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3181 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3241 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3301 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3361 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3421 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3481 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3541 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3601 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3661 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3721 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3781 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3841 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3901 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     3961 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4021 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4081 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4141 acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt
     4201 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4261 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4321 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4381 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4441 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4501 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4561 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4621 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4681 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4741 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4801 cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta
     4861 aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa
     4921 caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg
     4981 tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt
     5041 ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg
     5101 tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt
     5161 acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt
     5221 catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta
     5281 tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag
     5341 cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat
     5401 tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc
     5461 atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa
     5521 tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg
     5581 ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc
     5641 aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga
     5701 acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt
     5761 catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt
     5821 tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga
     5881 ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag
     5941 agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg
     6001 gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg
     6061 ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca
     6121 aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg
     6181 catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag
     6241 cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg
     6301 caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc
     6361 ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct
     6421 ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa
     6481 tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa
     6541 aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca
     6601 cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg
     6661 cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt
     6721 tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa
     6781 aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct
     6841 cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt
     6901 gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa
     6961 gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt
     7021 ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag
     7081 atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt
     7141 ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat
     7201 actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg
     7261 ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac
     7321 ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga
     7381 caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt
     7441 aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt
     7501 agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt
     7561 tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct
     7621 gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc
     7681 aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt
     7741 tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg
     7801 tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct
     7861 aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct
     7921 gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg
     7981 accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt
     8041 aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa
     8101 gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca
     8161 gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca
     8221 ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt
     8281 gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg
     8341 cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag
     8401 ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta
     8461 ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc
     8521 aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc
     8581 attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca
     8641 atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat
     8701 cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat taatgatctc
     8761 ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa
     8821 aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg
     8881 atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc
     8941 accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat
     9001 gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata
     9061 atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca
     9121 tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag
     9181 caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag
     9241 attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt
     9301 ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac
     9361 atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg
     9421 tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt
     9481 taaggtctat accaagttgc atacacacta aaaaaaaaag cgcacactaa acacacaaat
     9541 taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt
     9601 cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc
     9661 aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag
     9721 ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa
     9781 acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa
     9841 aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa
     9901 gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt
     9961 cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc
    10021 cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa
    10081 tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac
    10141 tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat attatgataa
    10201 ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg
    10261 gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa
    10321 ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg
    10381 attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg
    10441 aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc
    10501 atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct
    10561 tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca
    10621 ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa
    10681 ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag
    10741 caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg
    10801 taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac
    10861 cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc
    10921 ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct
    10981 tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga
    11041 taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa
    11101 ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag
    11161 ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca
    11221 gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt
    11281 tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa
    11341 aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc
    11401 cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc
    11461 aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa
    11521 cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat
    11581 gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc
    11641 aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata
    11701 catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc
    11761 cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt
    11821 tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg
    11881 taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag
    11941 gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag
    12001 aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat
    12061 tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga
    12121 gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc
    12181 tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata
    12241 agacatcact gcgatgatac cagggaatac accatccact ttattcagct tggctaattc
    12301 gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc
    12361 agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt
    12421 ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg
    12481 aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt
    12541 tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat
    12601 tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga
    12661 tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt
    12721 atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc
    12781 tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca
    12841 ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga
    12901 tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata
    12961 tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat
    13021 tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc
    13081 atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc
    13141 aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa
    13201 aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc
    13261 cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc
    13321 cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac
    13381 tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt
    13441 tcttccaaac cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg
    13501 tttgaatcat ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa
    13561 acgaagaaga tatttaccat aaaaaagact aggataaaat tcactcctag ccttcgatca
    13621 tttttatttt gaacgacaaa aaatatgatg ttcaataaca gacgatcttc attcgattct
    13681 tgatgtcatc ttttgttttt tttaatatat ggataaaaag tttgattaca tcgtttctat
    13741 tctagaattt ggtcgattct caattcttct tctggtgaga aatctaaatt atccatcatt
    13801 cgaacacttt cttgtaactg acttaaccga cttgctccga ccagtacact tgtaacagat
    13861 ttttgtcgca aattccatgc aagggccatt tgcgctaatg attgtcctcg tgatacggcg
    13921 attgtttgca gtgctttgat tttttccaga gttgatccaa cttgttcttc tgaaaggaat
    13981 ggtatttcct tacgatgtgc tctagaatct tcaggaatac catgtagata acgatttgtc
    14041 agcaaccctt gagcaagcgg actgaacgtg attgccccta gcccttcttc ttctaatact
    14101 ggagatagat cgtcttctag tagtgcacga gaaaacatat tgtagcgcat ctgatgaata
    14161 atgaacggtg cttcctttct ttttaaaatt tcagtcattt tttttgtatc ttctccatta
    14221 tagttggata taccaatata taatgctttt ccttgtctta caagttgcat caaagcttct
    14281 gctgtttctt ccaaaggtgt atcagggtct gggcgatgat gatagaagat gtcaacataa
    14341 tctagcccca ttctttggag actttggtca caacttgaaa tgatggattt acgagagccc
    14401 cattccccat atggcccttc ccacatatga taacctgctt tgctagaaat aatcagttca
    14461 tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa agtttttctc tgcagctcca
    14521 gcagggcctc cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct
    14581 cttaaaatct ctctttgatt atggatagga tcataatcac caaaattttg ccataatccg
    14641 agagataaaa tcggtaattt caaacctgaa tgaccggatt tacgatattc cattttttca
    14701 taacgtgcac tatctgcaat atacaatttt tctcctcctc atcaaatctt tttaaaacgc
    14761 tttctttttt ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt
    14821 ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg
    14881 tgcttgctgg atttttttat aacgtttcgg ttgttttttg taaaattgct gatgatagtt
    14941 ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt
    15001 tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgacttg
    15061 ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt
    15121 gggatcagta atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc
    15181 ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt
    15241 tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga
    15301 ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata
    15361 gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc
    15421 cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg
    15481 acgtagtgtt gcttctagtt ttgataactg ttctttaacg gatatatttt ctcttatgtc
    15541 attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag
    15601 attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag
    15661 aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa gcgattactt
    15721 catcccccta cagatttttc gtataaaaaa gagagaatcc ttatcgacag attctccttt
    15781 ttaatcaatt tattttataa atgaaaagtt aatttacctt ttatggaaaa ctattttaaa
    15841 aaataaacat aaatatgcag ttaatgtaac aagataagaa ataatagtcg ttactgtata
    15901 acttgtgctt actttaatat cagcaactcc tttgactccg cttatttgta ccaatccatt
    15961 agcattcacc tgctccttta caggttgatg atttatttct acattcgttc ctaaataagc
    16021 atatacagga agtgtgatta cttggctctt ctcattagtt actctatata tgatagaact
    16081 accagttgtt ttataaggaa ggtttaccca ttgactgcca atctttgctt cgtgttttat
    16141 tatatgctcc ttattctcca atgaagcgat aggagaataa tcatataaaa aataatccat
    16201 tcctttagtg gtatacgcct ctactgtttc tttgtttaca acaatatgtc ccggctgaga
    16261 aattattttt tgattgaaat tttttttact agaaacagtt aaagtgatta ataacaaaac
    16321 agtcataaat aaataaaata gttctcttgt cttattgtat cttctatttt tgaaaaatat
    16381 catcgttaat atcagagaag agaatattat ttgcaagctt aagatccgcc aaggaaattg
    16441 taacaatttg gctggcgtat tttgcagaag ataccatggc accaaactag ttgtagctag
    16501 ccaagtcaaa cacgtaccat aataaatctt tttttctatg tttgttaatt ttttaaataa
    16561 aatcagggga atacataaag ataacagacc aactagtccc attgagtaag tagttaagtc
    16621 gttgttcata gcttcagtca gactatcgaa cacattagat gcatgagcat ataaatttgt
    16681 cacgctaggt ttgttgattt tttggtagag catttgttca aacatcggat accaaaaagc
    16741 actcgtcaga accaatgtca ttcctgccgc actaaataat tttttaaata tagcaataaa
    16801 atctgcattc ttgaaacctt tagaaaccaa acgaataaaa ataaaaatga taattaatgc
    16861 tgtactcatt aatgttgata gtacatggct gtacactagc aaactcatac tcaatgctag
    16921 ctttacagac ggatactttt cttcttttcc agagatgatt tgataagcat aaaataatac
    16981 agggataaca agtgttattg cgatagcttc ggcaattgcg gaacgatagt agatgtctac
    17041 tgtccgataa gcagagaaaa tatagagaca actaaataaa aatgccgctt ttttttgttt
    17101 taaaaaccgc tcgccataat gataagctac gagtgttgta cacaagttca ataaccaaac
    17161 gtaaataata tagcttacaa tcaaattttt tgtcatccaa tataacatta cagctggaaa
    17221 aaaagtcaga aagggataaa aataatttac accatatcca gtataattga atgtctcata
    17281 attaatcgga ctggtaaaaa tagtcgataa ccccttgatt cgagataagt gaaaagaaag
    17341 atctccagtg gtataaatac tctgtgtatt tttgaaaaag atgaaagaat atatactact
    17401 aactataatt aatccactca atattactac ttttttattt attttcattc ttcttgttta
    17461 tctccttttt agtcacatat cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac
    17521 ttgaatacac cactaaacaa taagtcagaa tactgatcca taaagccatg agagcagttg
    17581 gagtatattt tgttgtaatt tctatcttat tttctccagc atttacatcc acattcacta
    17641 agccatgttt tttataagag tcatttacga ctttaccgtt tacttttacc tgtgtaccta
    17701 aataagcata tactggtact gtcacttttt gctttttgtt gcttgccact gtgtaagtaa
    17761 tgttattatc tgcagcttta tacttccctt tttcccacga ttctcctact tttacttcat
    17821 gtttttctaa gtgaggcctt tcttttatcg cttctgttgg ggcgtaatcg tataatcctc
    17881 ccatatctga tgttgtataa cgtgggagtg tttcttcatt gataacaata tgaccgtgtt
    17941 ttgattggat aacttttgcg taattttctt ttgctgctat agacgtgacg agtagaaaca
    18001 ccacagtagc gccaatgatt aaccatgaat attttttatt catagtcttt ttcataaaaa
    18061 caagcgttat gatcattgaa gaaaaaaaga tttggatacc taagatacgc caaggaaact
    18121 ggagtaagct tatcggtgtt ttttgaaaca aaaaccatgg aaataaatta gttacgcata
    18181 cccatgtaga aactacgcca acatagatga ttttttcttt tttctccatt tctttcagta
    18241 tgaataaagg aaaaatcaac gcaaccaatc ctaataaccc catagtgtat gttgtaatat
    18301 cgttatttaa cgcttgaatg atactatctg aaatagaaag tgcttctagc tgcaaatctg
    18361 ttttgaaggg actgtgtatt tcttgataat tcatctgctg caacatagga taccaaaaat
    18421 agctagttaa tgcacaagtc aaaaaaacag cctttactaa attcacgacc acaaatttta
    18481 tatcgttttt tatttgcttg cttaaaaaca aacgaactat aatcatacaa gcaatcaatg
    18541 cactacacaa tagtgtagtt aagacatgag tatacacaag tagacttata ctgacagata
    18601 aagcgatgac ttttttttct ctattgaata tcaatccata agcataatat aaaacagctg
    18661 ggataatcgt aagagcgatt gcttctgcaa ttgcggatcg ataatagata tcaactgttc
    18721 gataagttgc aaaagtatat aaacaactaa ataaaaaagc agcatctatc tttttaaaaa
    18781 acttttctcc ataataaaaa ctaatcagaa tcgtacaaat atttaataac caaatatata
    18841 gaacataact taatattaag ttatttgaaa ttccataaaa aacgactgca ggaattattg
    18901 taagaaaagg ataaaaatag ttcagtccat ctccataatt gttaaatgtt gtatagttga
    18961 ttggcccttc aaa
//
LOCUS       DAZAOG010000038        18745 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000038 DAZAOG010000000
VERSION     DAZAOG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.038"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..552
                     /locus_tag="ACHDLD_001139"
     CDS             <1..552
                     /locus_tag="ACHDLD_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN3362883.1"
                     /translation="EFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIF
                     VNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKV
                     KGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGY
                     FTSKGDLLYPTQLLDKIEFDYNI"
     gene            752..1066
                     /locus_tag="ACHDLD_001140"
     CDS             752..1066
                     /locus_tag="ACHDLD_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3362884.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1087..1464
                     /locus_tag="ACHDLD_001141"
     CDS             1087..1464
                     /locus_tag="ACHDLD_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3362885.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1474..2247
                     /locus_tag="ACHDLD_001142"
     CDS             1474..2247
                     /locus_tag="ACHDLD_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362886.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2269..3672
                     /locus_tag="ACHDLD_001143"
     CDS             2269..3672
                     /locus_tag="ACHDLD_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN3362887.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3854..5038
                     /gene="mobT"
                     /locus_tag="ACHDLD_001144"
     CDS             3854..5038
                     /gene="mobT"
                     /locus_tag="ACHDLD_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN3362888.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5035..5325
                     /locus_tag="ACHDLD_001145"
     CDS             5035..5325
                     /locus_tag="ACHDLD_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362889.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5322..5543
                     /locus_tag="ACHDLD_001146"
     CDS             5322..5543
                     /locus_tag="ACHDLD_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362890.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5585..6364
                     /locus_tag="ACHDLD_001147"
     CDS             5585..6364
                     /locus_tag="ACHDLD_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFN3362891.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6425..6925
                     /locus_tag="ACHDLD_001148"
     CDS             6425..6925
                     /locus_tag="ACHDLD_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN3362892.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6981..7619
                     /locus_tag="ACHDLD_001149"
     CDS             6981..7619
                     /locus_tag="ACHDLD_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFN3362893.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7691..8086
                     /locus_tag="ACHDLD_001150"
     CDS             7691..8086
                     /locus_tag="ACHDLD_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3362894.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8070..10523
                     /locus_tag="ACHDLD_001151"
     CDS             8070..10523
                     /locus_tag="ACHDLD_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3362895.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10520..12547
                     /locus_tag="ACHDLD_001152"
     CDS             10520..12547
                     /locus_tag="ACHDLD_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3362896.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12544..13566
                     /locus_tag="ACHDLD_001153"
     CDS             12544..13566
                     /locus_tag="ACHDLD_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFN3362897.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13583..14512
                     /locus_tag="ACHDLD_001154"
     CDS             13583..14512
                     /locus_tag="ACHDLD_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3362898.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14826..14873
                     /locus_tag="ACHDLD_001155"
                     /pseudo
     CDS             14826..14873
                     /locus_tag="ACHDLD_001155"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14889..15689
                     /locus_tag="ACHDLD_001156"
                     /pseudo
     CDS             14889..15689
                     /locus_tag="ACHDLD_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15927..16163
                     /locus_tag="ACHDLD_001157"
     CDS             15927..16163
                     /locus_tag="ACHDLD_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362899.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16170..18122
                     /locus_tag="ACHDLD_001158"
     CDS             16170..18122
                     /locus_tag="ACHDLD_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN3362900.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18194..18691)
                     /gene="dfrG"
                     /locus_tag="ACHDLD_001159"
     CDS             complement(18194..18691)
                     /gene="dfrG"
                     /locus_tag="ACHDLD_001159"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN3362901.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         6426 a         3107 c         3650 g         5562 t
ORIGIN      
        1 gaatttggaa tagaagttaa agattatttt attgacttag atttattaca gctaataaac
       61 agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa atttgttacc
      121 aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat ttttaagcac
      181 tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt atctagtagt
      241 gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac tacagcacat
      301 aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa cgggaaaatt
      361 cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc gagaaaacta
      421 ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga ggtgggttat
      481 tttacgagta aaggagactt gctttatccc actcaattat tagataaaat agaatttgat
      541 tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat cggacgctca
      601 gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg aaaataaaaa
      661 atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata agattcattc
      721 aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca ttcccaatat
      781 ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac aacgtcgaat
      841 aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga ttcaacgagc
      901 agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg aatccgacga
      961 aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg gcaatcgagg
     1021 atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa ggaaaggtga
     1081 aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt tggcacattg
     1141 aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac ggtttccgaa
     1201 gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat gatacaagtc
     1261 agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt ggaattaatc
     1321 aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt tgattggtac
     1381 gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa atataaaaaa
     1441 gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt gattttgaaa
     1501 gaaattccga aggatgaagc catttctttt attcggcaat accattattc aaagatactc
     1561 cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt aggcgttgtg
     1621 gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc cgacagttcc
     1681 cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga aatgaatcaa
     1741 accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa agaacatacg
     1801 gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg ctatgtctat
     1861 caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg tgataagcag
     1921 tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa tgctcgcttt
     1981 gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa agggattgag
     2041 aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc gaaaaaactg
     2101 ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt acgctttgaa
     2161 aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga taaacaggct
     2221 cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat gccattgctt
     2281 catgacagag gagagcgaat caaagccaac gataagcact tggtctatca ttttgtacta
     2341 atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag agcgttacaa
     2401 tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca tacgccatac
     2461 ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg gctttattat
     2521 cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt
     2581 ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt taaagattta
     2641 cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta tcgaatgaaa
     2701 gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga tcagcttttg
     2761 cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa aacgttgaaa
     2821 gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat tggcattgaa
     2881 gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg ggagttcgat
     2941 tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta ttttattctc
     3001 acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga ccctaaaaat
     3061 gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa agaagatatg
     3121 ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac catgaaacaa
     3181 caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc caattttctt
     3241 atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac caccgttatt
     3301 cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt aattcttgcc
     3361 tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt taacttccgt
     3421 gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag cgatacacaa
     3481 aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg aaaaagtgtg
     3541 attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg agaaatagga
     3601 aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg tacaggttca
     3661 gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct ctatctaaca
     3721 ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg atggtacaaa
     3781 ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc ttttttagat
     3841 tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag aaaagcgact
     3901 ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca gaccttacct
     3961 atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag atttactgaa
     4021 cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact atgtacggat
     4081 tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt tgaatatgga
     4141 ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc gtttcggtga
     4201 tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa
     4261 aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt atgatttctt
     4321 tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta tcaacgataa
     4381 aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag aatgcatttc
     4441 cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg atgaaaaacc
     4501 atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt tctgtatata
     4561 tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa
     4621 gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga aagatttatt
     4681 ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata tccgctttgc
     4741 agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt gggaatggtt
     4801 tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt attcttttga
     4861 acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg caagcatttt
     4921 agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca aactgacaga
     4981 aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga ttacatgagc
     5041 cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat
     5101 atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca agctggtgat
     5161 accatctata cctatgattt ttacaaggtg agattttata ataattatac caatcgagta
     5221 accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa aagctatatc
     5281 tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt gggcagaatt
     5341 tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt gttgtcattg
     5401 gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg attattgctc
     5461 taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat
     5521 ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa gtatatattt
     5581 tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc ttttaattaa
     5641 aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa
     5701 aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt ataaaggata
     5761 taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt ttgatgattt
     5821 agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca tatatgataa
     5881 acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga attacgaagt
     5941 aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg aagacgctga
     6001 gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca ctgcttttca
     6061 tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag acgaaaatct
     6121 atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac ctactgaact
     6181 tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct ctcttaatga
     6241 agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa agagagaagc
     6301 aaaactaata ataggtattt caagtacagt tacagattat attagcggat accttgataa
     6361 gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa ggatgtgata
     6421 agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag gtgaattagt
     6481 cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa tcggtttaaa
     6541 tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg tggacgaata
     6601 cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg aaggtacacc
     6661 tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg aagaattact
     6721 ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag atgtcgcccg
     6781 ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac aaaattatat
     6841 tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag ttacctctca
     6901 tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa attaataaat
     6961 agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta ttaaaactag
     7021 acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa cagcaaaatt
     7081 atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat gctctgttaa
     7141 aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat gctaattcgg
     7201 taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa tacagtagta
     7261 acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt ccgacagaag
     7321 ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta agtcaaagtg
     7381 attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct ttaaagggaa
     7441 atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg tatagaccca
     7501 gcttatatga acgattaggg tcagatacca atttaagttt cagatattca atgaatccag
     7561 atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa attgaatagt
     7621 taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt tacggaaagg
     7681 acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag aaaaagtgat
     7741 ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa tgacatggtt
     7801 tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt ctctgattga
     7861 cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca tgtgccaaaa
     7921 gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct atgctctacg
     7981 ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa tgaatgaatc
     8041 gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat atggaagata
     8101 atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt ccctataatt
     8161 acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt caattgattg
     8221 cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca agtattcgag
     8281 ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata gcttacgaaa
     8341 aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa gtagactatc
     8401 gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta aaaaatgttg
     8461 ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac aaattaatgg
     8521 gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg gaacagttgt
     8581 tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat gattttggtt
     8641 atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac gactaccatt
     8701 taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt ctcaaaccca
     8761 ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa gataccacta
     8821 cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc cctaatagtg
     8881 aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt atgaatgttg
     8941 aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa gaactgaaag
     9001 atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg gtggacgctt
     9061 tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct atgtacaaac
     9121 taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa cgttgtgatg
     9181 aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt ggcgatatgc
     9241 taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac tatattcaat
     9301 atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg attggtgaaa
     9361 attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat ctccaaccta
     9421 gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc gcttttattg
     9481 gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat tcagtactct
     9541 ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg aaagaaacct
     9601 tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac gacaataaag
     9661 ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc cttgccattg
     9721 atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt cctgtcctgc
     9781 gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg gtgattgatg
     9841 aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc gaaagctttg
     9901 tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa tccattagct
     9961 tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat tccaatagct
    10021 cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt gtcattagta
    10081 cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt gaccttgatg
    10141 aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga ttggttcgag
    10201 caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac gatttactag
    10261 atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact gacctagtgg
    10321 aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac aatcagaaac
    10381 gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt cgtgtagggg
    10441 ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc agaccgcctg
    10501 tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact atagttggat
    10561 aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca atgcagtaca
    10621 agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca agtatggttt
    10681 gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc catggaattg
    10741 gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta attttatttg
    10801 gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag cctatgcctt
    10861 agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga ttgcaggcat
    10921 tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc tttttattct
    10981 cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat
    11041 acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc
    11101 acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa
    11161 tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat
    11221 gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg
    11281 ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc
    11341 agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga
    11401 caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt
    11461 tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc
    11521 gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt
    11581 gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac
    11641 aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc
    11701 gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg
    11761 gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca
    11821 aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag
    11881 atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac
    11941 aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac
    12001 tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca
    12061 acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag
    12121 aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca
    12181 aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa
    12241 agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca
    12301 aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc
    12361 tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc
    12421 acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac
    12481 accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca
    12541 aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc
    12601 ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat
    12661 tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg
    12721 attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt
    12781 atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt
    12841 ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt
    12901 gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat
    12961 aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa
    13021 ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct
    13081 atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag
    13141 ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga aaccgtgcaa
    13201 gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg
    13261 cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt
    13321 tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa
    13381 gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact
    13441 catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat
    13501 ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat
    13561 aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa
    13621 accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac
    13681 gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat
    13741 ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca
    13801 gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt
    13861 ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt
    13921 gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc
    13981 tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt
    14041 actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag
    14101 ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac
    14161 aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac
    14221 agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta
    14281 tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat
    14341 taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca
    14401 agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc
    14461 gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt
    14521 ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc
    14581 ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa
    14641 tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat
    14701 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
    14761 ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc
    14821 ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg
    14881 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
    14941 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
    15001 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
    15061 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
    15121 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
    15181 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
    15241 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
    15301 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
    15361 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
    15421 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
    15481 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
    15541 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
    15601 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
    15661 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
    15721 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
    15781 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
    15841 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
    15901 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
    15961 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
    16021 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
    16081 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
    16141 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
    16201 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
    16261 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
    16321 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
    16381 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
    16441 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
    16501 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
    16561 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
    16621 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
    16681 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
    16741 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
    16801 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
    16861 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
    16921 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
    16981 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
    17041 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
    17101 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
    17161 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
    17221 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
    17281 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
    17341 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
    17401 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
    17461 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
    17521 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
    17581 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
    17641 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
    17701 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
    17761 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
    17821 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
    17881 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
    17941 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
    18001 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
    18061 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
    18121 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
    18181 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
    18241 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
    18301 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
    18361 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
    18421 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
    18481 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
    18541 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
    18601 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
    18661 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
    18721 catctaattt cttatttcta tcaaa
//
LOCUS       DAZAOG010000039        18740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000039 DAZAOG010000000
VERSION     DAZAOG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.039"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACHDLD_001160"
     CDS             256..531
                     /locus_tag="ACHDLD_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362902.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACHDLD_001161"
     CDS             544..2616
                     /locus_tag="ACHDLD_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN3362903.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACHDLD_001162"
     CDS             2784..4667
                     /locus_tag="ACHDLD_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3362904.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACHDLD_001163"
     CDS             4679..5626
                     /locus_tag="ACHDLD_001163"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN3362905.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACHDLD_001164"
     CDS             5646..6173
                     /locus_tag="ACHDLD_001164"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN3362906.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACHDLD_001165"
     CDS             complement(6236..6889)
                     /locus_tag="ACHDLD_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN3362907.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACHDLD_001166"
     CDS             7021..7863
                     /locus_tag="ACHDLD_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3362908.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACHDLD_001167"
     CDS             8021..8917
                     /locus_tag="ACHDLD_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3362909.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACHDLD_001168"
     CDS             8920..9633
                     /locus_tag="ACHDLD_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN3362910.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACHDLD_001169"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACHDLD_001169"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3362911.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACHDLD_001170"
     CDS             10168..10395
                     /locus_tag="ACHDLD_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN3362912.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACHDLD_001171"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACHDLD_001171"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3362913.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACHDLD_001172"
     CDS             complement(11181..11723)
                     /locus_tag="ACHDLD_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN3362914.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACHDLD_001173"
     CDS             complement(11960..12358)
                     /locus_tag="ACHDLD_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3362915.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHDLD_001174"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHDLD_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN3362916.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACHDLD_001175"
     CDS             13471..14238
                     /locus_tag="ACHDLD_001175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN3362917.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACHDLD_001176"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACHDLD_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN3362918.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACHDLD_001177"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACHDLD_001177"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN3362919.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHDLD_001178"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHDLD_001178"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN3362920.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAZAOG010000040        18450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000040 DAZAOG010000000
VERSION     DAZAOG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.040"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1396)
                     /locus_tag="ACHDLD_001179"
     CDS             complement(89..1396)
                     /locus_tag="ACHDLD_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN3362921.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1399..1980)
                     /locus_tag="ACHDLD_001180"
     CDS             complement(1399..1980)
                     /locus_tag="ACHDLD_001180"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN3362922.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2096..2193)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2552..3844)
                     /locus_tag="ACHDLD_001181"
     CDS             complement(2552..3844)
                     /locus_tag="ACHDLD_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3362923.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3841..4464)
                     /locus_tag="ACHDLD_001182"
     CDS             complement(3841..4464)
                     /locus_tag="ACHDLD_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362924.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4467..5303)
                     /locus_tag="ACHDLD_001183"
     CDS             complement(4467..5303)
                     /locus_tag="ACHDLD_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362925.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5320..6207)
                     /locus_tag="ACHDLD_001184"
     CDS             complement(5320..6207)
                     /locus_tag="ACHDLD_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3362926.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6215..7450)
                     /locus_tag="ACHDLD_001185"
                     /pseudo
     CDS             complement(6215..7450)
                     /locus_tag="ACHDLD_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8133..8816)
                     /locus_tag="ACHDLD_001186"
     CDS             complement(8133..8816)
                     /locus_tag="ACHDLD_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN3362927.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8834..9451)
                     /locus_tag="ACHDLD_001187"
     CDS             complement(8834..9451)
                     /locus_tag="ACHDLD_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3362928.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9461..10285)
                     /locus_tag="ACHDLD_001188"
     CDS             complement(9461..10285)
                     /locus_tag="ACHDLD_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN3362929.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10278..10838)
                     /locus_tag="ACHDLD_001189"
     CDS             complement(10278..10838)
                     /locus_tag="ACHDLD_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362930.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11012..11968
                     /locus_tag="ACHDLD_001190"
     CDS             11012..11968
                     /locus_tag="ACHDLD_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3362931.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(12273..13229)
                     /locus_tag="ACHDLD_001191"
     CDS             complement(12273..13229)
                     /locus_tag="ACHDLD_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN3362932.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            13333..13935
                     /locus_tag="ACHDLD_001192"
     CDS             13333..13935
                     /locus_tag="ACHDLD_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3362933.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(14023..15522)
                     /locus_tag="ACHDLD_001193"
     CDS             complement(14023..15522)
                     /locus_tag="ACHDLD_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN3362934.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(15538..16062)
                     /locus_tag="ACHDLD_001194"
     CDS             complement(15538..16062)
                     /locus_tag="ACHDLD_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN3362935.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            16360..17109
                     /locus_tag="ACHDLD_001195"
     CDS             16360..17109
                     /locus_tag="ACHDLD_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3362936.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17100..17885
                     /locus_tag="ACHDLD_001196"
     CDS             17100..17885
                     /locus_tag="ACHDLD_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3362937.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            17854..>18450
                     /locus_tag="ACHDLD_001197"
     CDS             17854..>18450
                     /locus_tag="ACHDLD_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3362938.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYL"
BASE COUNT         5513 a         3921 c         3221 g         5795 t
ORIGIN      
        1 aaacggcaaa atacgaggaa ctatttttcc gtttgtttct ctaacaatca ggtcaaaacg
       61 ctgatttcac aacctttctt ttgctctatt acttcttgaa ggcttcttcc aatatttctt
      121 catctttttt caatccatta aagatcaaat tcaatatgat agccgtaatg ctactcatta
      181 cgatgccatt tccagtgaac atttgcaatg tttgcggcat ttgactgaat aatgtcggcg
      241 taacattgaa acccagccca aatccaacag aaacagcaac aatcaataga tttttatcat
      301 tgctgaaatc aacttccaac agcatacgga ttccttggac tgccaccatc ccgaacatga
      361 ctaacattcc tccaccaagt accggctcag ggatgatttg tgctaacgca ccgattttag
      421 ggaataatcc taaaacaatc aagaagaatg cagagaaata gatgggtttt cttgttttga
      481 ttcccgaaag ttgaaccaat ccgacatttt gagaaaagcc agtataaggg aatgtattga
      541 aaataccgcc taagataacg gcaagtcctt ctgcacgata tccctttttc aattcttctt
      601 ctccaacatt tttcccagta atgtcaccaa gtgcaaaata tacacctgtt gattctacca
      661 tgctgaccat ggaaatgatg atcattagta aaatcgacca tacgtcgaaa gttggtttac
      721 caaaataaaa gggttgtgga acatggaaca acggcgcatc tccaatcgcg ctcagatcaa
      781 tcattcccat gaaagcagct aagatgcttc ctccgactaa accaatcaat accgcgattg
      841 aacgaatgaa cccttttgcc agcatttgca cgcctacaat taggagaatc gtcacaaatg
      901 caagtagcag atttgttttg tcaccaaaat cagctgctgt tgcattacct ccacccattt
      961 tctcgattgc tacaggaatc aaagtcaaac caataacggt gataaccgtt cctgttacta
     1021 taggagggaa tagtttttta attttagaaa agacaccaga aactaacaca acaaagatcc
     1081 cagaagcgat aatagatccg tagatagctc ctactccttc attggttccg atcaagatca
     1141 gtggagctac tgcttggatc gcacatccta aaacgacagg caagccaatt ccgaaaaatc
     1201 ggttaactgt caattgcaat aatgtcgcta atccacacat aaagatatca attgaaatca
     1261 aataagtcat ctgttcttga ttaaagccta gtcctgtacc gatcaaaaga ggcaccgcta
     1321 ctgctcctgc atacatcgct agcagatgct gcagccctaa aaccgcagct tttccatttt
     1381 gtgtttcttt ttccacgatt atgcgtcctc ctcaagaaac tcaacttgtc cattcgtcaa
     1441 ggaagcaata cgcgcaagag aaacgactct caatcccatt tcttcaagta actgacgtcc
     1501 atcttggaaa gacttttcga tgacgatccc gattccttct acttgcgctc cagcttgctg
     1561 gcataattcg atcaatcctt ttgccgcttg tccgtttgcc aagaaatcgt cgataatcaa
     1621 tactttgtct tctgaagata aaaatttacg agaaatagaa atcgtacttg tcacttgttt
     1681 cgtaaaagaa taaacagaag ctgtcaataa ttcttcatcc atcgtcaaac tttttgcttt
     1741 tctagcgaag atcattggaa catctaattc tcttgcagca aataaagctg gtgcgatccc
     1801 agaagcttca atggtcacaa ctttcgtgat tccttgttct ttaaatactt ctgcaaaccg
     1861 ttttcccatt tgttccatca atacagggtc tacttggtga gtgacgaagc tgtcgacttt
     1921 taatacgccc tcgcctaata tacgtccgtc tttttgaata cgttcaacta attctttcac
     1981 tttatttgct cctttatcat ttattaatac cgaaataacc gcttttgtgc aaaaacctac
     2041 tatgtttctg ttaaagaaaa acaccctcca ctttatcata agaaaagaag ggtgaaatag
     2101 ccaatctttt acttatagtc cagcatttac ggtactggct agaaactgtc atccatatta
     2161 tgacgatata taagtaggta tgtaaaacta ataatagtgt aaattcattt tcttgcaact
     2221 gttttgcacg tgaaggcagt ttgccatatt tgaataaaaa atgttgtttc aaatattgaa
     2281 aacagtctaa cactactttc tgaaactgac aacgtggttt acttattttt ttgtaaaatc
     2341 taatgatttt taatgcatta ttcctttttg ttcgggttta aatagttatt tttattttct
     2401 cctatctaaa aaagcagccc acataaaaag aaggcatgac aaaaagcatg tcatagcccc
     2461 tttttccgaa taaatagtgt tcaaaagaca gcctatcggc aataaggcaa gattttataa
     2521 actacgggaa gtaatttccg taaatctatt cttattcttt taagctgaca agctcgtcaa
     2581 tcactactgc ttttcctgaa gcaatagaat gatttgctgc gattcctgtc aaaatcgaca
     2641 aaataccatc tacgtgagtt gctgcccgat tccacggatc ttcttctttt tcttcaccaa
     2701 atagatcatc taataaacgc ggatctcctc cgccatgtcc gcccgcttct tgttcgacga
     2761 ttacctcata tggctcaccg aacatcggat agacatggat ggactgaaga acggctgctc
     2821 cttcatcctt tttatctcca cctgcattga tgtaagactt ttcgatcact ttcatttcga
     2881 tccgtccttt gctgccattg aaagaaacat tgaatccttc ccacggcata tacgcgttta
     2941 gcgaatacgt caaaatcgca ccagtttcat atttgaccgt tacgcctagt gtatcttcaa
     3001 tagagatatc atcggaaaat acactgcggt cacgaatata tccactgtct ttttctgctt
     3061 ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc tatagcaaat ggatcagctt
     3121 tagctagttc gcttccatag catcgactgt aaaatatttc tactccgcgt ttctccgcat
     3181 tatgcatccc ataaaagttt aatccaccga atgcaaatac cttttgggga cggctgttca
     3241 accaaaaatt cactagatca aaatgatggg tagatttatg aacaagaagc ccgccgctat
     3301 tgtgcttatt tcgatgccat cttctgaaat aatctgcccc gtgttgcgta tttaacagcc
     3361 attcgaagtg gatagaaaac acatcaccaa tcacatcact ttctatcagt tcctttatct
     3421 tcgtgtgata cggtgcataa cgataattaa aagtgactcg caggcttctt cccgtctgtt
     3481 tttggaccgt tattatttct tgagcttttt cctcatcgat agtcagaggt ttttcgcaaa
     3541 taacatcata tccttttttc attgcacgaa tgatataatc atgatgcgtt cgatctactg
     3601 acgtaataat gacttcagcc ggccgtacgt catccagtag ttgctcaaat tgtgatggca
     3661 tacatgtctt tacttttttc tgtccatact tgttcacaag tatccgattt gcataatcca
     3721 tccttgtttg agatacatca caaaaaccga ctagttcaca agtttgatga tacgtagagg
     3781 caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc gacttgtaca tattttttct
     3841 tcatttaaag tcattctcct tttcttttaa gcaaaatact gtgacaaatg gcagccctgg
     3901 atacagtaat gcagtgagta aaaaatcagg ctgtatacac cagaacagtc catacaaaag
     3961 gcataaatac cacagaaacc ccttgacgtt ttttgacaac agccagatat tccttttgat
     4021 caaataaaca agactatctg ctttatcttc tttgcagata tcaattagcg tactaaaaaa
     4081 actatgggat acaaaaagca acagacacaa tagaaaagga atcagatacc aagctccaat
     4141 ctttcccact aataaatacc aatcaactgc aaaaataatg acaaacagct gaaacaacgt
     4201 aatgatgaaa cacatccgtt tgattttccc tttcctccaa catacctttt tttctgccaa
     4261 gtaatggaca gatagataat aagaaggata catcatcaaa aaataaatca gatagacaac
     4321 acgagcagac agcactaatc ctaaagtgaa caaacatcct agaactaaag cgaaaagagc
     4381 attactgata agactggtta aaaaaatccg acagacatag tagttgagct tgtaaagaaa
     4441 catctcttct attggctttt tcatattcag cccttcagac cgcttgtact gatcccctgg
     4501 acaagatatt tattgaagaa taagaaaatc agaaaaaccg gtagcagcga caacgtagac
     4561 atcgcaaaca tactcgcgta agtcgtacta gatccagcgt ctgcaaataa tttgatggca
     4621 atcgataccg tgtaagatct cggcttgttt aaataaatca atggtcccat gaagtcgtcc
     4681 catttccaat aaaactggat gatgatcgtc gtgatgatcg aaggtttcag caaaggaaaa
     4741 ataattttgg taaagacact atacttgctg catccgtcaa taatcgctga ttcatctaac
     4801 tctttgggga tgctgaccat gaattgcatg atctggtaaa tgaaaaatgc ttgtccaaaa
     4861 aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat tcagccgatt gtaaataata
     4921 aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg ttactaacat gacgacgaac
     4981 cagaatcttt ttcctttgaa atcgattctc gataatgcgt aagcaataca agtcgagctc
     5041 agaactgtcc ctatcgtcga aactgcagag atgacaaacg aattcctaaa gaaagtaaaa
     5101 aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat tttcccattg aaaagtctct
     5161 ggaatcaaat tcagcgaacc actcataatc tcttgatccg ttttaaaaga acctgcgatc
     5221 atccataaga ccggataaat catgactaga cctaatagca aaataaaacc gtattgaaac
     5281 catttcgtta ctgtattatt catttcttcc tcctctcatt cacacaacga cccatttttt
     5341 tgatgatttg aaaatcagaa aggtgacagc tcccataatc aacagcatga tccacgaaat
     5401 agcactagca taccccatag aattaaaaga aaaagcttgg ttatacacat aaagagcata
     5461 aaagttcgtg gcatcattcg gaccaccttg ggtaatgata aatgcctgcg taaaagtcat
     5521 aaacgccgcg attgtctgca taaccaagtt atacaaaatg atcggcgaaa gaagaggtaa
     5581 cgtgatcttg taaaacgtct gccatttgtt cgctccatcg atctctgccg cttcataata
     5641 cgagcctggg atctctttga tacctgcagc aaagatgatc attgccgaac cgaattgcca
     5701 agcagacatc aaaacaagag ggatcatgac cagtttttga tcgctaaacc aggaaagcgc
     5761 aggtgcgcct aatcgttcca ggaaaatatt cactaacccg tttttagaaa acaactcccg
     5821 ccaaactaga gcgatagaaa tactgccacc gatcaaacta ggcacataat acaaagaacg
     5881 aaagaaagtc gttcctttga tattcctcgt taaaagataa gcaatcaata gaccaaagat
     5941 cagtttcaca ggtacagatg caaatacata gagaaaagtg actcggatgg agttcaaaaa
     6001 acgggcatca ctcagcatct ttatataatt atccaagccg atccatgaga ctggatttcc
     6061 caaacgataa tcggtgaacg aataataaag tgacaagatc atcggtatca ctgttatcaa
     6121 tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg accacattgt cttgatccaa
     6181 gaaccgtttt agtttttctt ttttcatgcc ttccttctgc tgttacttct tcataaatga
     6241 ctttttcaac taaaagattg tattggtctt gaatttccgg attcaaagca ctatcaaaga
     6301 cattcacttc gccgttgtcc atatcggaaa tcatttctaa ataatcaaat gtcgcttgca
     6361 aagttgcatc atctgcacga gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac
     6421 gttctccttt taaaatttca ttggcttctt cactgttttg aaagaaatca ataaacttag
     6481 cagcttcttc tttatgggct gagctttttg ccatactcag catttgcgaa gaacgtaaag
     6541 ggataccaga ttctccatct gcttttctgc gaggtggatt aatcaactga atctccatat
     6601 cttcaggagc ccctgacaat atttctgtca actggttact tgatagaaac accattgccg
     6661 cttctctatc cctaaccgcc tgaaaagaca gatccgtcgt acttgcagct ccaccacctg
     6721 gttctggatg ggcccctttg tctaccagat ttttacgcag ctggaaaaaa tctgttagat
     6781 agtctggatt ttcaaatccc aagctgttag atagatcttc ccgattataa aaattcaatc
     6841 ctttctcaac ttgtggtatt tgaataatac agccagctat aaaatcttcg aatcttgaaa
     6901 tagcatagat tcctaattct tcagtgattt gatagctgat ttgttcaaat tcatcccatg
     6961 tccaatttga agctggttca gggatattta attgttcaaa tatattttta ttgtaagcaa
     7021 tcgcaaacga attcaccccg ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg
     7081 ctaaatagga ttcatctgca ttagaaatat caattatccc ttgctccaca tattcatcta
     7141 atggttcaat ctgattgatg tattgaggaa attgattgtt cagttggaaa atgtcccaga
     7201 tatcatctga tgctgctagt gtacctaatt ttgtgtaata tccttcataa tcataaaatt
     7261 cataatcgat cgtcacatgg ggattttttt cttcataaag ctcgattact tttactgttg
     7321 catcgtgtcg gacttgcgaa ccccaccaag ccatgttcag cgtgattttt ttgtcatctt
     7381 cttgcccaca agccgtcaag aagaataaca taacacttaa agctaagatg attctcattc
     7441 ttttcttcat tctctgcacc tctacttcca ttgttatttt tcaatatact gtatttttat
     7501 acagaacata tttattaaac gatctttttc aaggggaata aaaaccaaga cttgccttat
     7561 cgttctgcct aggatgaaca actcaatact ttcaagaaaa attagatagt gctgattctt
     7621 ctttcatcgc taaaaacact tcttgcttca atgatgggag gataattacg caaagaaaaa
     7681 aggatatcag acaactgccc aatatccttg acatagctaa ataagcatgc ttcttcgtgt
     7741 gcgctcttcc ctaaaaaaca gaaaaataac tagtaattct gtatgaaatt aggtacaata
     7801 tacacgtcga tgcacaagca ttgactatgg caacttgatt ggtcagttcc cgctgacttt
     7861 tcaagccccg acaatttagc tgctactaaa ttgtcgggtg ccttttttag tttttattcg
     7921 tttttcattc tatagaaagc gcttcaataa atcaagacat ttttttgtta gaatgctaat
     7981 aataacaaaa ggttttaact cttcttatgt acattacagg taaaaaaaca aaaaaatttc
     8041 tctttttttt gaattctgaa caaatagtac tggagtacaa aagacgagga agagccacta
     8101 tctgctcttc ctcgtctttt gattctcacc tgttattcat gattcttttt ctcagtttcc
     8161 cgatattttt ccattttacg actaaaaagc tctccgctac ttggtggcgt atatgtgatg
     8221 gcatttgctc ctgcttgaat ggtctcagca atactttctt ccgttggacc acctgtcgca
     8281 atgataggaa gttcaggata ttttttacga aaatggcgta ctgcttctgc agtttcttta
     8341 cctgcactga tattgataat cgaaatacca gctgctaact tttcatctat ttctgtatat
     8401 ttcgacgtta ccgtactgat aactggaata tcaactactc gacaaacttc ttttactgtc
     8461 tctaaaggcg taggaccgtt caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg
     8521 ctcatgtagg aagaacgagc accttgtgtc aaaccaccac ctacaccgga gaaaactgga
     8581 atgtcagctg cctcaataat gcttttagta atcgctggat gaggtgtaaa aggatacaca
     8641 gcaattactg cattcgcatt tgtattacgt atgatcgcaa tatccgtagt aaagatgatc
     8701 gaacggatca attttccaaa tatcttgatc ccgcttgctt ctaaaatgat ttccggtact
     8761 cttacgatat cttttctcaa ttccgtcatg atttctggcg taaattgttc tgccatcagc
     8821 cacttctcct ttgctatctc cggtcatact gattcaattg atacgtctcg atgacctgga
     8881 tgattttttg cgcataaccg gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg
     8941 cttcctgata gttggtggcc gtaatcactt tttcatacag cgcaggattc caatctactc
     9001 cttggacgaa cagttgtgta tggtcatcca tggactgttc ccatgaatca tacactttga
     9061 agtctccttg gatagttatc cattcttcgt taacaaattc ctttgtttcc aggcttactt
     9121 tcttctgatc cccataagcc ttgatcccga aaagattatt gtatttgcta gccaaggtac
     9181 tttttcccca atttgattct aatatagctt gtcctaaaat gatactagga agcactccat
     9241 aaccatcttg tagttcttgg gcatgcggaa caagccgttc aataaattgc tgatgggatt
     9301 gcgcttcatt ttcttgctga ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa
     9361 caaaggctaa gagtatcact gctacaccgg caaccactgc tggccaacgg atattcttgc
     9421 gcttttgctt tttccacttt tttttcgcca tgatcaattc ctacttttca tttaattcat
     9481 tcagatattc ttctagcgtc aattggtgtt tggtgatata ctcagcgttt tctttaccga
     9541 cataacgtaa gtgccaaggt tcatatgtga tcttagttat atcttcgtgt ccttttggat
     9601 aacgaacgat aaatccatac tgcgcagcat tatctgcgat ccatttagct ccaggctgat
     9661 ctccgtaact tgcatccaat acagtagaag gataactgtt gtaccagttt tgatcgacga
     9721 catcgactgc cagacctgtg tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag
     9781 taatctttgt cgcctcctct tctgatacac cttgacgact catcacatct tggacatttt
     9841 gttcgaaaac ttgttgttgg gatgatacag accggtaagc tgagaccatc actaatggaa
     9901 atccagctgc ttcagcagcc ttggcaaatg cttcatattc tggagcaatg cgtttgtcga
     9961 tcaagtatcc gttacttaat gatgccaatt gagtagactc atcaatttct ttttctaatt
    10021 tgtgatctgg accaaccaaa actagcttcc aatcgtctgt tttaacatct ggcaattgtt
    10081 tggacgtacg atcttttgtt tctttgtcct ttaccttgct gctggttgtt tgactgttct
    10141 tttctttact agcagttgtt ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa
    10201 catgcgaatc atcatcgcta aaaataacat agcccattgc tgcgatcata atcacaactg
    10261 ctccaaatcc taaaaattta ttcacttgaa aaatcctttc gttgttcttc acgttcccca
    10321 ttcgcctcat gaacgtaagt ttcttccaga ttttcattca cccaatcgag aagttcgatt
    10381 ttttctcctt cgatctgttt ttgaacatct gctggcagac gaaaattcaa aatatcatca
    10441 tagccccatt catcatattg aatacttact ttcaagtcaa ggtatctacc agcctgctga
    10501 ttatttttca aagggatcag ctcgactttt gctgctttgc tgctaagcca tttttgcgcg
    10561 atgcgtgttt tgatttgctt gtactctgca acggcaacgt ttcgtttttc gggaaaaata
    10621 atcgtttgac gcaaaatctt ttgcgttagc atccattcat aatcagtgaa aagatttttt
    10681 actttctgca tcgcttcaga tggcagattt ttctcgccat ctttccattt ttgccattgt
    10741 tctggactga tcagcaagta ttcatctaaa aaattttttt cagtttcaaa ctgttctaat
    10801 aatacactca taataagatc aatatatact tgatgcacgt ttatcccctc cttttaacta
    10861 ttctacttaa gttttgatca aaaagcacct cttaaatcgc tgctttcatt ttttttaatg
    10921 tttcaagtcc tctcctcttt attcttcgtg atttttgtta taataataga cagacaagaa
    10981 actcaatcca actatgaagg agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg
    11041 tcttggtacc atcgcacatg agtttgccga aagcttcaat caagagacga gcgaactaac
    11101 agcagttgct tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc
    11161 aaaagcttac ggaagctatc aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat
    11221 tgctgtacca aatagacagc atatcgacca tatcttggca gcattggaag caggcaaaca
    11281 cgttctatgt gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt
    11341 agcagaagaa aagaatttga tcttagcaga agcgatgaca atcttcaaca tgccacttta
    11401 tcagcagtta cgctcaataa tggatacagg aaaactgggg gctttaaaaa tgatccaagc
    11461 acctttcggc agttacaaag aaccagaccc taaaaaccgg ttcttcaatc cagaattagc
    11521 tggaggagct ctgttggata tcggtacgta tgctgtatct ttcgctcgtt tctttttgag
    11581 ctctcaacct gaagtagttg cctctacaat ggttcctttt gaaacaggcg tggacgaaca
    11641 atccgtcact atcttgcgta acaaagaaaa tgaacttgct gctgtcagct tgacttttca
    11701 ggcaaaaatg ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga cgattgctga
    11761 ttacccgcgt gctgatcgtg cggagatctt atttacagat ggtacaaagg aattcatcga
    11821 aagcgggaat acatctgaag caatgaatta cgaaataaag aatatggtac acatgattga
    11881 aggtgacttg cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa tcttggatca
    11941 aatgcaagtt ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat
    12001 caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gctggactta
    12061 ttaccgcaga ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca
    12121 gctccgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg aatattgtga
    12181 cttgtgaccg aggagttgac aggagaacac cgtttaaaga agaggttgtg aaatcagctt
    12241 tttgacctga atattagctg aattcacaac ctctattttt ggattttttg ttgggagaat
    12301 tctgccttga tttcaataaa acttttaaaa gactgcaaaa actggaacaa ttctgctcga
    12361 tcctcgaatt cagggcgact agtcggcaga ggcatttgcc gatacaatac ataaacttct
    12421 tcaatacgtg ttaaaatttg tctgccatca tttgactctg aaaatgtttc tgcagtataa
    12481 atcaataaac catagatatg ttttccataa taagctggtt gctggattct ctctagattt
    12541 tccagcatgt ctttaatcac attggcctgt gctcttctca tagagaagta agtttcgtag
    12601 tatagcggct gtctgagcca gtagttttct tgatgttctc tggcatcttc ttggctttcg
    12661 cgaatgaatg tcagtaaatg ttcgcatttg atctgcacat cttctttatt ttctgataaa
    12721 agaaattctg ccatttgtct caaaatattt cgaaattctt tttcgatctg tatttggttt
    12781 tccttcagct gcttcgtacg atctggcatg taaacatttg caagcaaagc aaatactaat
    12841 ccaatcgcca ttaatcccgc ttcattgccg atcagctgcc aagacatgtt ttcctccact
    12901 aaataatgag tgaccaaaac cgaatttact actatccctt cggttaattt gaagcgagag
    12961 gcaagcggga tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata cccaacagca
    13021 aaaaaactga acagtgctgt tgccgtagct aatataaagg ctgcgattcg attcatgccc
    13081 gtacgcaatg tagagcgctt ggtatttcct acacttaata atgcaatgat ccctgcagct
    13141 ggccaataca acagcgataa gctacccgct gtgaccatcg aaaagaagac actgatcact
    13201 gttttgactg ttcgcattcc tatacgcatt tacttccccc ttttttattt taattatact
    13261 tgaataatga aaaactagct atcagacaaa agtttaggta tgatagtaaa aagaccaaaa
    13321 aggagaatct ctatgcaatt tggccgtttt cgactaggta tgcgtacgat caaaagtgcc
    13381 ttagctgttt ttttatgtat cttgtttttt catttgactc aacgaggaat cccgatgatt
    13441 gctgcattat ctgctgtttt ttctttgcgt caggacttga ctacaaccgt ttcttttgga
    13501 aagtctagga tcatcggcaa tacattaggt ggtacattag ctatcattta tttttttgtc
    13561 aaaagctatt ttcacaatga ttttttggta gaattgatct tgcttcctat tctagttatt
    13621 attgtgatcg tcgtgtcaga tggtattaac aataattccg gtatcatttc cgcaatagct
    13681 acgttgttgc tgattgcgtt gagcatccct caaggcgaat ccgctttgta tgctatccaa
    13741 cgtgtactag atacttttat agggacattc attgcaatcg gtatcaattt ctttcttcgt
    13801 cctcctgaaa cagaaaagaa ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa
    13861 gaacgattgc taagaacaaa tttacaagag atagaagcta agatccaaaa acaaaagaaa
    13921 aatagcgagg actaattttc ttttgcaata aaataaaaaa atggagagaa gaactttaaa
    13981 aagtcttcct ccatttttga ttgaacggtg aagtgtacta ctttattctt ccttcatcga
    14041 gttttgttct gcctcttttg gcgcttcata catatttact tggctagtag tcccacgtgt
    14101 cgacaaattc tctacgattt ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg
    14161 agtagaagaa agcaaatttg tggcataatt tgtcactcgg ttcgctccac tggtttctcc
    14221 tgaactgctt cctgtatcga caacagagat tttttcgata tttcctagcg gctgtgctgc
    14281 ttcgcgcatc aattgcggta gcatatcgat aaccatactc aagacagctg cttcgccata
    14341 ttccttgaag gcattggcga tcttttgttt tgcttcggct tctgcttttc cttttgctag
    14401 tgctgcttcg gcttctgctt gtccagctaa acgtgtctta ttggcttccg cttctgccag
    14461 tgcttccaca cggaatcgtt ctgcttcggc ttcagtcact tctttgactt tttgagcttg
    14521 tgcttcttgt tctttagcgt accgatcggc atctgctttt ttcttcactt ccgaatcata
    14581 ttgcttttca cgacgtgtga tttctttttc ttccagttcg atttgttttt ggcgttctac
    14641 gattttaatc tgcatttcct gttcgattac ttgttgttgt gctcgggcac tttctaaatt
    14701 gtaggcttgg tctgcttttg ctttagcaac atcttgttct tgtttgtagg ttgctaattt
    14761 cagttctttt tcttttaatg attctgcgat ctctgtttgt cgctgtaatt cagctgcttg
    14821 tgattctttt tctgcttgag cttttttgat acgcgtttct tttagagctt ccgcttctgc
    14881 tatatctgca tctcttttga cttgggcgat tcttggtttc cctaaagagt caagataacc
    14941 gtttttgtct ctgacctctt tgatagtgaa agaaacgatg atcaacccca tttttgcaag
    15001 gtctacagaa gcgacttctt gtacgctttg actgaactta tcgcgatttt gatagatttc
    15061 ttctacagtc atagaaccaa gaatcgagcg caagtgtcct tctaatactt ctcttgcttc
    15121 attttccagt tcttctcttg tcttgcctaa aaactgttct gctgctgtag caatctcttc
    15181 tacagatgaa ccgattttga tgatcgaagt accatcacac atcaccggta cgccttgttc
    15241 ggtatacact tcaggggtgg agacgtccaa tttactcgat aatagactga tccgattcga
    15301 acgttgaaaa actggcaaga cgaatgcgcc acctccacgc acaattttga ttttgttatt
    15361 gctttcatca atgtgcacat ttttgcttcc taaataactt ccactgatga tcaacgcttc
    15421 atccggtttt gctgtctggt acttcgctac aaagacgatc agcaacatca gtaaaatcaa
    15481 agctgcgatt gcgatcgcga taaaaacaac tggtatttcc atttaacaca cgctccttta
    15541 agcttttatc atgctttcat aaggcatgac ataacagatt ctttccttga tctcaatgat
    15601 caatactttc gttccccgct cagctgtctg ttcttggggg ctgtaaaaac aagctggacg
    15661 actaatggaa ccggtaacac ttttcaactg aatttctccc atacctcgta cagggatcgg
    15721 tgtgatcaca acagctactt gtccttccag agttttttca gaagacgaca tcgttgattc
    15781 ggcatgtttc atcggcatca atatatagaa attaagcaaa aataccaaca ctatcgatat
    15841 gcctccacta gcaataaata aaaataaaga tgattgatct gttaaacgct cacctagaaa
    15901 accaaataag cttgtgaaag ttaaccaagg gataataagc atcggatcaa gcgggccatc
    15961 aaaatcgaat acatctccaa aaataatcaa taggactgcc aatcctgcac aaatcagcaa
    16021 tgtataccaa taaatcgtct caagtgcgat tccccctatc atatacacgc ctccttccat
    16081 ttctacgtct atcataccaa tgtttgaaaa ttctgtatag cttcttttac cgactcagtg
    16141 gtgtttttta taggaaggtt gtgtttatcg ttgacaattt gttaaatttt tgttttcatg
    16201 ttaaaatttt cacaataaat tctttcgagg tagcctatgt ttcctttttt taagaaaaaa
    16261 ccattaaagc tccagaacac gatcatcctt gtcgtttgtt tctccacatt cgtttctttg
    16321 attgtttctg ccatattgat caggaatttt attctttcga tgaaccgatc tagtgtgtca
    16381 gataaaatct ctagcattgc taaaatcgtt gccaatgata aactcatcca aaaaaagctg
    16441 cagactggtg acccgacaca tgcgattcaa ggaataacaa atgaaatcat gattgcatcg
    16501 aatttagatt ttatcgttgt catggaccct gaaggcattc gatattctca cgcagaccca
    16561 agttacatcg gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa
    16621 gaatttttct ctgaaaaaga aggaatgtta gggttcggct tccgctattt cgtccctatt
    16681 tattatgatc aagagattat tggtgcagtt tgtgttggct taacaaacaa cacgattgac
    16741 aatcaagtca aagatggaca aaaaatgatt gttttttcat tgattattgg acttagcgta
    16801 ggcatcatag gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa
    16861 ccacaagaaa tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa
    16921 ggaatcttag ccatctcttc tgataaaact atcctcttga tcaatacacc tgcaaaaaac
    16981 attttctacc aaacagagca gctattggat ttcgaagtag gaaaaaaatc agtgataatc
    17041 tatatgacgt tttgtttaaa gagacgattg agcatcaaac gaccgttcgg actcaatcca
    17101 tgtatttaaa tgggatagaa actatttcta gcgttacacc tctttttagc aatgaccgtt
    17161 ttttaggagc agtcgcaact ttacgaaatc gatcagaagt aatgaaatta atggaggagc
    17221 ttggggggac gaaacaatat ataaactcca tgagagctca aacccatgaa ttcatgaata
    17281 aacttcatgt catttctggt ttgattgatt tgaagaagta tgaagaagtc acccatttta
    17341 tccagcagct aaatcaaaat tataaaaaag agattggcca agtctcaagt ctgatcaaag
    17401 tacctgcaat tgccggtttt ttattaggca aaataaacga agccaaagaa caatctgttg
    17461 aaatgctgtt agaaagcgat tcttttatcc ctaaaatgga gatggatgac tccatccatt
    17521 cacttttgca agtgttaggg aacttacttg ataatgcaaa agaagccgtg ctacaaaaac
    17581 atgaaagaaa cggacaggtc cggattcatt taaactatga cacagaagct gaagtattca
    17641 tcgtgaccgt ctgcgatggc ggtgtcggga tatcagaaga aatctcagaa aaaatattta
    17701 cgctaggttt ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc atccattcaa
    17761 tcgtcaaacg ccacggtgga tttttagaat tttccaaaca aacaaatggc ggtacaatcg
    17821 tctatctaga attaccaaaa aaggaagatc aaaatgatga caaatgtcct gataatcgaa
    17881 gatgatccaa tggttgcaga attgaatcgt acttttgtgg aaagcatccc taatatgaac
    17941 gtaatcgaaa gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca
    18001 gatctgctgc tgcttgatgt atacatgcct ggcgaaagtg gtatccattt tttatcttgg
    18061 cttagacagc aaggaaatga tatcccagtg attttgatca ctgctgcaga tgatatcgaa
    18121 accataaaga atgtgatccg ctacggtgcc ttagattatt tgatcaaacc gttcactttg
    18181 caacgttttc agctggcgat cgaaaagtat cttgctttct ctcagacaat ccaaacgact
    18241 gaaaaagccg atcagaaatt gatcgatcaa gtattcaacc acaccactcc cattcacgaa
    18301 ataccatcat tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt
    18361 gcatttattc taaaagaaga tactattttt tcaacaaaag aactggctca aaaaagctca
    18421 ctgtcacgta tatccgtcaa gaaatatttg
//
LOCUS       DAZAOG010000041        18446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000041 DAZAOG010000000
VERSION     DAZAOG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.041"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..564)
                     /locus_tag="ACHDLD_001198"
     CDS             complement(109..564)
                     /locus_tag="ACHDLD_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362939.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="ACHDLD_001199"
     CDS             complement(718..1926)
                     /locus_tag="ACHDLD_001199"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN3362940.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="ACHDLD_001200"
     CDS             complement(2038..2370)
                     /locus_tag="ACHDLD_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN3362941.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="ACHDLD_001201"
     CDS             2518..3393
                     /locus_tag="ACHDLD_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3362942.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="ACHDLD_001202"
     CDS             complement(3475..4056)
                     /locus_tag="ACHDLD_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN3362943.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="ACHDLD_001203"
     CDS             complement(4062..5321)
                     /locus_tag="ACHDLD_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3362944.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="ACHDLD_001204"
     CDS             complement(5323..6369)
                     /locus_tag="ACHDLD_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362945.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="ACHDLD_001205"
     CDS             complement(6604..6879)
                     /locus_tag="ACHDLD_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN3362946.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="ACHDLD_001206"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="ACHDLD_001206"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN3362947.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ACHDLD_001207"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ACHDLD_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN3362948.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /gene="cmk"
                     /locus_tag="ACHDLD_001208"
     CDS             complement(9978..10658)
                     /gene="cmk"
                     /locus_tag="ACHDLD_001208"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN3362949.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="ACHDLD_001209"
     CDS             complement(10707..11342)
                     /locus_tag="ACHDLD_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN3362950.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="ACHDLD_001210"
     CDS             complement(11408..12811)
                     /locus_tag="ACHDLD_001210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN3362951.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="ACHDLD_001211"
     CDS             complement(12798..13829)
                     /locus_tag="ACHDLD_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3362952.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="ACHDLD_001212"
     CDS             13860..14081
                     /locus_tag="ACHDLD_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN3362953.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="ACHDLD_001213"
     CDS             complement(14246..14983)
                     /locus_tag="ACHDLD_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN3362954.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="ACHDLD_001214"
     CDS             complement(15136..16476)
                     /locus_tag="ACHDLD_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3362955.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="ACHDLD_001215"
     CDS             complement(16713..18236)
                     /locus_tag="ACHDLD_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN3362956.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5196 a         3930 c         3110 g         6210 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacatt
//
LOCUS       DAZAOG010000042        18270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000042 DAZAOG010000000
VERSION     DAZAOG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.042"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHDLD_001216"
     tRNA            12..84
                     /locus_tag="ACHDLD_001216"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHDLD_001217"
     tRNA            88..160
                     /locus_tag="ACHDLD_001217"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHDLD_001218"
     tRNA            168..249
                     /locus_tag="ACHDLD_001218"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHDLD_001219"
     tRNA            267..339
                     /locus_tag="ACHDLD_001219"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHDLD_001220"
     tRNA            351..422
                     /locus_tag="ACHDLD_001220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHDLD_001221"
     tRNA            439..524
                     /locus_tag="ACHDLD_001221"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHDLD_001222"
     tRNA            535..608
                     /locus_tag="ACHDLD_001222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHDLD_001223"
     tRNA            618..691
                     /locus_tag="ACHDLD_001223"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHDLD_001224"
     tRNA            711..783
                     /locus_tag="ACHDLD_001224"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHDLD_001225"
     tRNA            811..884
                     /locus_tag="ACHDLD_001225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHDLD_001226"
     tRNA            898..971
                     /locus_tag="ACHDLD_001226"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHDLD_001227"
     tRNA            995..1084
                     /locus_tag="ACHDLD_001227"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHDLD_001228"
     tRNA            1097..1170
                     /locus_tag="ACHDLD_001228"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHDLD_001229"
     tRNA            1189..1264
                     /locus_tag="ACHDLD_001229"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHDLD_001230"
     tRNA            1266..1336
                     /locus_tag="ACHDLD_001230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHDLD_001231"
     tRNA            1350..1423
                     /locus_tag="ACHDLD_001231"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHDLD_001232"
     tRNA            1448..1535
                     /locus_tag="ACHDLD_001232"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHDLD_001233"
     tRNA            1557..1628
                     /locus_tag="ACHDLD_001233"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHDLD_001234"
     tRNA            1651..1740
                     /locus_tag="ACHDLD_001234"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHDLD_001235"
     tRNA            1753..1826
                     /locus_tag="ACHDLD_001235"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHDLD_001236"
     tRNA            1845..1917
                     /locus_tag="ACHDLD_001236"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHDLD_001237"
     tRNA            1921..1993
                     /locus_tag="ACHDLD_001237"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHDLD_001238"
     tRNA            1998..2068
                     /locus_tag="ACHDLD_001238"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHDLD_001239"
     tRNA            2081..2154
                     /locus_tag="ACHDLD_001239"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHDLD_001240"
     tRNA            2166..2237
                     /locus_tag="ACHDLD_001240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHDLD_001241"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHDLD_001241"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN3362957.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHDLD_001242"
     CDS             3826..5298
                     /locus_tag="ACHDLD_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN3362958.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHDLD_001243"
     CDS             5381..6169
                     /locus_tag="ACHDLD_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3362959.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHDLD_001244"
     CDS             complement(6236..6904)
                     /locus_tag="ACHDLD_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN3362960.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHDLD_001245"
     CDS             7019..8731
                     /locus_tag="ACHDLD_001245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362961.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACHDLD_001246"
     CDS             8734..10503
                     /locus_tag="ACHDLD_001246"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3362962.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACHDLD_001247"
     CDS             complement(10708..11274)
                     /locus_tag="ACHDLD_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3362963.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACHDLD_001248"
     CDS             complement(11274..12230)
                     /locus_tag="ACHDLD_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN3362964.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACHDLD_001249"
     CDS             complement(12230..12886)
                     /locus_tag="ACHDLD_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN3362965.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ACHDLD_001250"
     CDS             13256..15274
                     /locus_tag="ACHDLD_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3362966.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /gene="leuS"
                     /locus_tag="ACHDLD_001251"
     CDS             15701..18115
                     /gene="leuS"
                     /locus_tag="ACHDLD_001251"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN3362967.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         5592 a         3407 c         4020 g         5251 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct
//
LOCUS       DAZAOG010000043        18188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000043 DAZAOG010000000
VERSION     DAZAOG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.043"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..458
                     /locus_tag="ACHDLD_001252"
     CDS             135..458
                     /locus_tag="ACHDLD_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN3362968.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            640..2010
                     /locus_tag="ACHDLD_001253"
     CDS             640..2010
                     /locus_tag="ACHDLD_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN3362969.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(2140..3441)
                     /locus_tag="ACHDLD_001254"
     CDS             complement(2140..3441)
                     /locus_tag="ACHDLD_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN3362970.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(3795..5786)
                     /locus_tag="ACHDLD_001255"
     CDS             complement(3795..5786)
                     /locus_tag="ACHDLD_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN3362971.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(5930..6196)
                     /locus_tag="ACHDLD_001256"
     CDS             complement(5930..6196)
                     /locus_tag="ACHDLD_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3362972.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(6706..8832)
                     /gene="pnp"
                     /locus_tag="ACHDLD_001257"
     CDS             complement(6706..8832)
                     /gene="pnp"
                     /locus_tag="ACHDLD_001257"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN3362973.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(9020..9289)
                     /gene="rpsO"
                     /locus_tag="ACHDLD_001258"
     CDS             complement(9020..9289)
                     /gene="rpsO"
                     /locus_tag="ACHDLD_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN3362974.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            9540..10103
                     /gene="def"
                     /locus_tag="ACHDLD_001259"
     CDS             9540..10103
                     /gene="def"
                     /locus_tag="ACHDLD_001259"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN3362975.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(10356..10877)
                     /locus_tag="ACHDLD_001260"
     CDS             complement(10356..10877)
                     /locus_tag="ACHDLD_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN3362976.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(10880..11722)
                     /locus_tag="ACHDLD_001261"
     CDS             complement(10880..11722)
                     /locus_tag="ACHDLD_001261"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN3362977.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(11715..12440)
                     /locus_tag="ACHDLD_001262"
     CDS             complement(11715..12440)
                     /locus_tag="ACHDLD_001262"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN3362978.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            12616..12912
                     /locus_tag="ACHDLD_001263"
     CDS             12616..12912
                     /locus_tag="ACHDLD_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3362979.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      13168..13342
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            13480..15072
                     /locus_tag="ACHDLD_001264"
     CDS             13480..15072
                     /locus_tag="ACHDLD_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3362980.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            15085..15825
                     /locus_tag="ACHDLD_001265"
     CDS             15085..15825
                     /locus_tag="ACHDLD_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3362981.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            15993..17342
                     /locus_tag="ACHDLD_001266"
     CDS             15993..17342
                     /locus_tag="ACHDLD_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3362982.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(17470..18060)
                     /locus_tag="ACHDLD_001267"
     CDS             complement(17470..18060)
                     /locus_tag="ACHDLD_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN3362983.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         5230 a         3697 c         3495 g         5766 t
ORIGIN      
        1 aaaagtattc aattattgtt gcatttttcc tgattcttta ttagaatgaa ccctcgttga
       61 agtaagtatt tctagtttat cctaggatta attataaaaa taaaacattc gttgattcga
      121 aggaggttaa acagatgttt gaaagttttg atagcagccg caaccgttac gcttcagttg
      181 gtgtcgtatc aagtttgcca gatgaattga ttgacagtat atggtttatt attgacctag
      241 atcttaaggg agtcattcca ttagaaaata tattaacctt tgatttgatc gaaaatcgtg
      301 gaaaagtgac gatgcatttt tctcaagcag gaagcaatgt ggaaatggat attgatttgc
      361 cgtttgccta ttcgaaccaa tttcctgctc aagtatacgc ctatgatgac ggtacgagag
      421 aaacgatcct tttaccaagt gagattagac aatattaagg tgatagataa acgtggatgt
      481 cccttatttt acagaccagg ccaagttgtt gattctttga ctcaattctt ggcttctttt
      541 tatggagttt cataaattat cattgatttc taattggtat tctcaggata aacgctatat
      601 actactaact aaattttaaa gatataagga gtttaaaaga tgcgcgattt aacaaaagga
      661 agccccgcta aactgatttt gatgttcact gttcctttac tgatcggaaa tgtttttcag
      721 cagttctaca atatgatcga tatgatcatc gtgggtcaga cgctaggtaa aaatgcgctg
      781 gcagcggttg gtgcaactgg aagcctgaca tttttgatca ttggctttgc ccaaggttta
      841 acagctggtt tagccattat taccgcacaa cgttacggtg caaaagatta tcggggtctg
      901 aagaaaagtt ttgctgctag tgttgtcatt agtcttgtcg tcactgtgat tttaactgtc
      961 ctaagtctcc tgtttattcg tccgatgctt caattgatgc aaactcctcc tgaaatactt
     1021 gatcaagcac aaacatttat ttcaattatt ttattaggga tctttgcatc agtcagtttc
     1081 aatttgcttt ctaatgtcat tcgcgcactt ggtgacagcc gcacaccact attctttctg
     1141 atcattgcgg tcatcatcaa cgtcgtactt gatttgatct ttattatcta ttttggcatg
     1201 ggtgtggaag gtgcagctat tgctacagta atcgcacaag tttcttccag cgtactgtgt
     1261 ctagtgtata tcaagaaaaa aatgcctttg ttacagttac gcaaaaaaga tttcaaattt
     1321 gaaaaagaag agtttgctgt acacctaaat gctgctttgc caatggcgtt tcaatcttcg
     1381 atcatcgctg ttggtgctat cgtgttacaa gcagcactaa atagtctagg gacagatgtc
     1441 gtagctgctc aagccgctgc tggtcgtatc gaccaatttg caaatcagcc gatgatgtca
     1501 tttggtattg caatggcaac tttcactgca caaaactatg gtgcgaaaga atatggccga
     1561 atcttgcaag gggtacgtca aactttactg atgagtgtcg gatttagtct cttagcaggt
     1621 gctatcgtca ttttcttcgg tcatttcttt gttcaattgt ttgttagccc atcagaaaca
     1681 cgtgtatttg aattagcaca aacctacttc aatatcaatg ggtctcttta ctggatctta
     1741 gcagtcctgt tcattttacg ctacacactt caagggcttg gacaaagcaa gatccctacg
     1801 atcgccggta tgatggaatt gcttatgcgt tcttttgcag ctatcgttct tacaggaatg
     1861 tggggatacc caggagcagc agcagcttct cctttagctt ggatcggttc tgttgccgtg
     1921 ttgctttact cttatatgcg ttcgatgaaa caattgaaac acatgggtga agaggaacat
     1981 ttcacattag aacatgcaga agcaagatag ccttgattaa aagaaatcgt tgcggctaaa
     2041 aatgaaaact tttattgaaa atatcttctt cgtttaccga aaacaggttt tttagataac
     2101 aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt tattctgttt ttctttccgt
     2161 ggactctact ttgaaataat cgatatgtgc atgctggtat tctaataccg tgaaacggaa
     2221 tgatgcaatc tcaatgattg ttcctgcttt tatttcttta tatcggctag aagcaaagcc
     2281 cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc tctacatgaa aataacgctg
     2341 gaaatcatct aatgttagtt tgccggagat gatataagag ccatctgact gttccgctat
     2401 caatgcttcg tccaaatctg tctcatctcg gatttccccg acgatttctt ctaaaatatc
     2461 ttcaatcgtc actaagccgc ttgtccctcc gtattcatct tttaagatag caatatgctt
     2521 accctttttt cgcatgatcg tcaataattt ttttataggg atcgtttcag ttgttacgat
     2581 cggctcttca atcaactgtt ctactgtcag ctggtcatct ttataagatt gcttgatcaa
     2641 atctggcaag gtgacataac ctaaaacatt gtcttttgat tctaagataa ccggatagcg
     2701 agtatgtcct tgttcaatcg aagcttttat cgcttctttg acggtttctg aagcttcgaa
     2761 tacttgcatg gacgtacggt ctacttgtat atcttttgcc aaggtatcat caaattcaaa
     2821 cacgttttcc aaataataat attcttcttt ggtcaactct cctttttcat aagaattcaa
     2881 tgcgactaat gtcagctctt cttgagacaa tgtctcttct ccttcagata cgagacgcac
     2941 acctaatagc ttccctagcc ctgcagctga atgattcaat acccaaataa aaggaaacat
     3001 tactttataa aaataatgaa gtggtttaac tacgaacaag acgaccttct ccgtattgac
     3061 gatactatag ctcttaggaa gcagttcgcc aataaccaca tcgatatacg taactaaaat
     3121 aaatcccagc gccacagaga ttgtactgct aatagccgaa ctaaaaggta gtttaccgat
     3181 cactggttct agtaaagccg cgacagatcc ttcaccgacc cacccgatga tcaaaccggt
     3241 tagcgtaatt cctaattgac tagctgataa atagttatct aaatggtgtg taacatgaag
     3301 agctagcttg gcgttcttta cattttcctt ttctagttgt tccaatcttg atcgtctgat
     3361 tttcactaaa gcaaattcac ttgctacaaa aaatgaagta attgcaatca tcaaaaataa
     3421 aataatgaaa ttgataacca tattctcccg cctttctctt tttctattgt accctttttc
     3481 tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa taaaaaagga gatgcggcat
     3541 ttttcagcca catctcctta tcacttcaaa atcattgcgt tacttgttgc taaacagctt
     3601 tttcatgacc tctgataaaa acggtattca aaagcttgca tctacggcat taagcccaaa
     3661 cagatgaaaa acatgagaag ctgtttttat ctgctcgttc ttactgcttg ttgcagtaca
     3721 gctttttcat gacctctgat aaacggtgtt caagtagctg acattcgaca ttagttcagg
     3781 tcaaagcgct actttcacaa ccgctctttc aaccatgttt tccgagtatt tccaatgata
     3841 agcggaacag tttcatgttt tacttcgctg tactctagcc caaaccatgt ttctggcgag
     3901 gtagcgattt ttttgattgc cagttttgcc tgcatgattt gacgttgcca ttttcctaga
     3961 gttgtcacat tggaaactac ttcatcaatc aggacaaatt ggaaatcgcc aacttctcgc
     4021 cctggtgtca gagagtatct ttgtggctgt tttggcaaac ggccttgttt catcaaatca
     4081 tggatgactt ggcgaacata tacgttgatt tcctgttgga ccctgaaacc taagaacaga
     4141 ttcacttgga caataaaatc agtgtccatc atatctactt gatattcttt cgtatatggc
     4201 tcatccgtca cttccacatg aacaaaccaa tagactcgtg ctcttttagg ccgtttatct
     4261 aaaatcgaat agatgaactg tcggccaact ttatcttcag ccatatcagg aaccaagaag
     4321 accacatttg tctgatagat tggtaaagaa gtatcatcac gtaattctct aagctgcggg
     4381 atataatctc tcaaagacac atcctcggtt gacgattcgc gaataatgtt cccttgttcc
     4441 caaataaaca tcacgactaa gatgatcaaa ccaatcagaa cggctacaaa accgcctcgg
     4501 aagaatttga caacacttga gataaagaaa acgacttcta tcgcaccgaa gaaaaaggta
     4561 actaaatatg caagccaacg agaatgccca ttttggataa gataaaagta aagcagtgct
     4621 gttgtcatca acatcgtcac agtgatcgat agaccgtaag cagcttccat atgatgagac
     4681 gttcggaatg tgacaaccac tagagaacag gcgatccata aaatcagatt gactgctggg
     4741 atatacattt gcccgatact gttcccagga tacatgatct ttaaccgagg cagcagtttc
     4801 aacttgattg cttctgaaac tagcgtaaac gaacctgtaa tcaatgcttg cgaagcaatg
     4861 actgcagcga ctgttgcaaa agcaacgccg atcacgatcc aactttgagg catcatttgg
     4921 aagaatggat tcaggttctt gatttgctgt gcttcagggt tttgataaac attcaacaac
     4981 cacgcagcct gcccaaaata attgataacc aaacaaattt tgacgtaagg ccagctcaga
     5041 cgaatatttc gtttgccgac atgtccaaga tcggaataaa gcgcctctgc acctgtcgta
     5101 gccaggaaaa tatttccaag aatgaacaag cctaatttgt tatctgcact aaaaagcagc
     5161 tggatagcat aataaggatt cagtgcacgg ataactgtcc agtcttgact gaagttcatt
     5221 aatcccatta cgcctaaaaa agtaaaccaa gcgaacatga ttggtccgaa tgcttttccg
     5281 acaatatctg taccaaagcg ttggaccatg aataaaacaa aaataataac taaagtgata
     5341 atgacgatca cgttctggtt attaccgaac cggtcaaaaa actgcggaat tcctcgcaag
     5401 ccttcaatcg cagtcgttac cgtcacagca ggggtcaata ccccatctgc tagtaaggca
     5461 gctcctccga tcattgcagg aatgatcaaa tatttcccac cttttcggac aagtgtgtag
     5521 agtgagaaaa ttccaccttc accatggtta tctgcattca atgcaatcac gacatatttg
     5581 attgttgtca gtagagtcaa tgtccaaaaa atcaatgaga ctgatccgat aacaaaatca
     5641 ggattgattc ctgacaatcc gccattatct tcaacaatcg atttcataac gtaaagcgga
     5701 cttgtcccga tgtctccgta gacgactccc atcgctacaa gcaggccagt cattgatatc
     5761 tttttggtat tgtgcgttgt tgccacgttt ttccctcatt tcttgttata tggatatcct
     5821 ttaggtaagc ttgaatgata gatgatattc ttatccatga cacccgcttt cctaaattgc
     5881 ttctaattat ccaagtaaca cactgaagtg tcaacttatt tgaataaaat tattctttct
     5941 ttggacgttc tctgatacgt cttgccacaa atgaataaag agcaaggcaa aaaattgcac
     6001 ccaataatgc tggtatcaaa taaaaaccgc cgagttccgg tcccagtggt cctagtaaaa
     6061 attcgccgag ccaactacct aaaaaaccga taatgatatt tccagatacg cctcccggga
     6121 cttcctttcc taaaattgcg ccagctatca aacctaatat accgccgata aataacgacc
     6181 ataaaaaatt cagcatctcc tttccctcct ttatccatat ttctcacttt tattttacgc
     6241 tttattcaga aggactccaa aaatatgcgc caagaaactc cagaaaaaaa gaagttgtaa
     6301 caaaagtgtt cagcttattt ccgtagaagc tgcttctgga acaccgtgaa tcagaggttg
     6361 tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
     6421 ttgccgtttt ttgagctaat atcgagggac agctgattga acatcgttta ttggagaggt
     6481 tgtaaaaaag ctgttctgca tcaagtaata agaacagcca aataaaaaca gctcctaatg
     6541 tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
     6601 aaaaaaggac tgtgacacgg cttttgtcac agtccccttt tttcgaataa ttattgtccc
     6661 cctgtcacct tcacgatcac aagtacatta ttcctatgaa ttaaattatt tttcttcatt
     6721 tttcgttgaa ccatcttcgt tcaacattgc ttttcttgac agatttacgc gtccttgttt
     6781 atcgatttca gttactttca caaggatctc atcacctagt ttaacaacat cttcgacttt
     6841 attgacacga tcgtttgcta attgggaaat atggacaagt ccatcttttc ctttgatcaa
     6901 gttgacgaat gcaccgaatt tttcgatacg gacaactttc cctagataaa cttcaccaac
     6961 tttgacttct ttggtcaaat cttcgatgat cttgatagct tttttgatca tttctgcatc
     7021 agctgaagca atacttactt ttccatcttg atcgatatcg attttcacac cagtttcatc
     7081 gatgattccg ttgatcgtat cgccgccttt accgataaca tctttgattt tagcaggttc
     7141 gatctggatc atttcgattt ttggtgcata ttggcttaat tcttcacgtg gagcagcaat
     7201 cgttgaagtc aattcttcta agatttccat acgtgcttgt tttgcttgag ttaatgcttc
     7261 agttaggatt tgttctgtga ttccttgaat cttgatatcc atctgtaaag cagtgatacc
     7321 atcttttgta ccggcaactt taaagtccat atcgccaaga tgatcttcca gcccttggat
     7381 atcagttagg atcgtatagt tttcaccatc agaaacaagt cccatcgcaa taccggcgac
     7441 tggcgctttg attggtacac ctgcatccat caatgctaat gtgccagcac agatacttgc
     7501 ttgggaagaa gaaccatttg attccaatac ttctgctact aaacggatcg tataaggaaa
     7561 ttcttcttct gaagggatta cttgtgctaa cgcacgttca cctaatgcgc catgtccgat
     7621 ttcccgacgc cctggagagc ctgcacgtcc tgtagaacca acagaaaatt gagggaaatt
     7681 gtagtggtgg atgaatcgtt tgctgacttc cacccctaaa ccatcaatga tttggtgctc
     7741 tcctagaggt gcgagtgtac aagctgataa agcttgtgtt tgtcctcgtg tgaacaaacc
     7801 tgatccatgt acacgtggca ataagctgac ctctgatgac aatggacgga tttcatccaa
     7861 tttacgcccg tcaggacgga tcttatcgat ggtgatcaat tcacgtacga catctttttc
     7921 aagatcttct gtgatttgtt taacttcttt caacaagtga tcttcatctt catgacctgc
     7981 aaatttctca gcataagctt cgcggatatc gatcttcacc tgctcgatat tatcttcgcg
     8041 agcaagtttt tcttcagtca ttactgctgc tttcattgta gcgtaagaag catcaaagat
     8101 ttcttttttc aatacttcat caacttgcag taaagtcact tccatttttt ctttccctac
     8161 tgcttgaacg atttcttctt ggaaagcaac taactctttg atagcgtcaa aaccaaacag
     8221 caatgcacca agcatgtctt cttctgaaac ttctttcgcg ccgctttcaa ccatattgat
     8281 ggcttgtttc gttccggcaa cagtcaattc gatatctgtt tgttctgctt gttctactgt
     8341 tggattcaag acgtactcgc catttacacg accaacttct acgcctgcga ttgggccgtc
     8401 aaatgggata tctgagattg atagtgctaa agaagaacct agcattgctg ccatagctgg
     8461 cgagcagtct gtttctacac tcatcacgat gttcgttact tggacttcat tacggaagcc
     8521 ttctgcaaac atcgggcgaa ttggacggtc gatcaaacgg gcagtcaatg ttgcttccgt
     8581 acttggacgg ccttctcgtt tgatgaaacc tccagggatt ttccctgctg catacatttt
     8641 ttcttcataa ttgatcgtta atgggaaaaa gtctgtatct tttgcttctt tagaggccac
     8701 agctgcactt aagactactg tatctccata acggactaaa acggctccgt ttgcttgttt
     8761 tgctagctga ccgacttcga tttgtaaggg acggccgccc catgtcgttt tgaatacttg
     8821 tttttctgac ataacttctc cttttccttc tcgtgaaagc aagacgctac taaacaaatg
     8881 aaaactgccg aaagccgtct tttgagcagc tttcatttgg ttagtaggag tccatctagc
     8941 ttgatcacgg aattttactt aattgatata cgagtaaaaa aagtgagatt catgtgaatc
     9001 ccactctttt tgactaaaat taacgacgca agcctaaacg tttgatcaat tcgcggtaac
     9061 gttgaacgtc agttttacgt aagtaagcta acaagttacg gcggtgacca acttttttca
     9121 ttaggccgcg gtatgaatga tggtcttttt tgtgcacgcg tgcatgttca ttcaagtggt
     9181 tgatttcttc agttaataca gcgatttgta cttctggaga accagtatcc ccttcatggc
     9241 gagcgtattc gttgatgatt tcattcttgc gttcttttga aattgccatt tctttcacct
     9301 ctttcgatta ttcccctgcg actgagtaaa acgttggtga ttcgcttaac caagtggcag
     9361 gtcatgtgcc tagagcacac agctatactt tacctgaaac cgggttgaaa atcaagtagt
     9421 taatcattaa gaaaaggtag ttttcaaaca atcttactac tacttttcca tttttgtggt
     9481 aaactataat gcgaaaatag acttgaaaca cagtctttta agaaaggggt acatattcca
     9541 tgattacaat ggatgatatc attcgtgaag gaaatccgac cttacgcgaa gttgcaaaag
     9601 aagtttctct tccattaagc gaagaagata tctctttagg aaaagagatg cttgagttct
     9661 taaaaaacag ccaagatcca atcaaagctg aagaactaca cttacgaggc ggtgttggct
     9721 tagctgctcc tcaattagat atttctaaaa gaatcattgc tgttcacgta ccaagtcctg
     9781 acccagaggc agatggacct tctctaagta ctgttatgta taatcctaaa attttgagcc
     9841 attctgttca agatgcttgt ttaggggaag gtgaaggctg tttatctgtt gatcgagaag
     9901 taccaggata tgttgttcgc catgccaaaa tcactgtgtc ttattatgat atgaatggtg
     9961 aaaaacacaa gattcgtttg aagaattatg aatcgatcgt cgttcaacat gaaatcgacc
    10021 atatcaacgg tgtgatgttc tatgaccaca tcaacgatca aaatccattt gcactaaaag
    10081 aaggcgtatt ggtgatcgag tagagaattg gcaggttcct tcgatactat gctacaagaa
    10141 agctttataa aaaaggggag tgctcaaaag ctgcacttcc cttttttatg tttctccact
    10201 aatcgaaatt tagatagaca tctagaaatc gcaattccaa aaaattctgt gtattggata
    10261 cgcatctttt cttatttttt cttatttctt ccatatgggt gtttatctca gagatgatag
    10321 aatagtttct aggataaaaa gtagcagcta cgatgttatc cttcgtagag gattcctgct
    10381 tcagctagtt tttgtttgat gtttttcagc atatgctttt ctgggtagcg gatcgtatgc
    10441 agatggatgc cgcctgttaa ttcggataac aaagtagcat tacttttttg ataacgtctc
    10501 ataaaggaat cgatatcttg tggcgttttg atgtgcaaag ttcctgctaa ctcaccgtaa
    10561 aggggatgtt ccacaattac atcaatgatt tcaccaccat tttcaacgat tagtctcagt
    10621 tcttcttctg tttgatcttt tccatgctgg acagcgattt tactgatgtg cccagtattg
    10681 gtttcttctg tttcgaggat ataacctctt gctgttgcca cgatactttt acctgcagct
    10741 cgtagtaaag caatatcgcc tacaatgatc tgacggctga ctccaaaagt tttcgcaaac
    10801 ttgctagcac tcactggttt ttctgtattt gccaaggttt gcatgatttg ttttcgccgt
    10861 gtctctcctt cgatcacact tattcctcct tcattttgtt ttggtattcg cgcgagtgtt
    10921 tcatcaaatc aacatacgtg tctgttattg tttccttata tcccttattt ttcagcagac
    10981 taacaacttt atccaatact tgctgctctc tttttgcatc ataaactgct tggttttctt
    11041 cttttttcac ttgtcctatt ttagttaccg tatccattct tttttctaat aaagcaacca
    11101 actctcgatc gatcatatcg atcttatgac gtaatgtttc aagagactct tctgttagtt
    11161 cttcctctac tgtcttgatg atacgagcag gatttcctgc aatcactaca ttgtcgggaa
    11221 aagatttggt cacaaccgaa cctgcgccga ctactacgtt gtcccctaga gtgacacccg
    11281 gcgtgacgat aactcctccg cctaaccaaa cattattacc gattttaatg ggttttgcgt
    11341 actccagacc gctgttacgt ttcactggat gtagtggatg cgtcgccgtg taaagctgga
    11401 cattcggagc gaacatgcag ttatcaccga tttcaatcgt gctgacatct aaaaatgtac
    11461 aattgaaatt tgcataaaaa ttctccccaa caaatatatt atatccgtaa tcaaaattga
    11521 taactggctc catatagatt tttttccctg tacggccaaa agtctctttc aacaattggg
    11581 atcgcagttc cgctgattct gcttggttga tgatttggct ttgacttctt gcaaacatac
    11641 ggtcggcaac cagctcagga tcattggcaa aataaagctc gcctgcaatc attttttctt
    11701 tttcggtttt gaattcagtc ataatctgct cctaattctt gtattttttt gaaaacaacc
    11761 gctccgtctt cttgaataaa cgtataaggc tccgctaaat aaacaggcgt ttgattgatt
    11821 actaagattt tggctgacgg ctggcgaaac tgtaccagat cacaaaacgg atgaacttga
    11881 aaactcgttc caacaatgac gatcagctct gcttggctga cggcataagc cgcctgctcc
    11941 atgactccat cttgaaaccc ttcaccatat aacacgatgt ctgggcgtat ttgtcctccg
    12001 catgcttgat gtttgtcgct gtgaagatat tctttccagt ctaccgtttg ttgacatttt
    12061 cgacaatgac attgatacag actcccatga aaattgacga gttgcgggct tttagccttt
    12121 gcgtgcaaac cgtcgatgtt ttgggaaaca acccaaacat ttttttcttt cgctagatgt
    12181 gctatttcca aatgaatgat attgggtttt gcttcaggat gataaatctt tttgacaaat
    12241 tgatagaatt tatccggttc attcaccatt gcttgatgac tcagcaagta ttccggctgc
    12301 tccatcccgt ggtagattcc tgacaaagat cgatagtctg gcactccaga aggtgtggat
    12361 acccctgcac cggtcaaaaa tgtaatttgc tgataagcta atatctcttt tgctgcttcg
    12421 tctactgtta attgtttcat gttttgtcac ctcactttcc attatagccc atactcgaac
    12481 aaatttattg ttgaaaagtc agaaaaatgg gaataaaaaa gtgttatggg catttgagca
    12541 atttatcaca atgttcactg acaaaagaat gaaaacgtgt tacaatactg ctgtataaaa
    12601 gtgaggtgaa tcgatatgtt accattcttt atttattggg gcgttattct ggcttgtatc
    12661 gcctggttag cgttaagcgt ttatttctct gttttttatc ttgtacgcaa agaaaatgga
    12721 aatctttggg cttttgcttt attcaacgtg attgccgcaa ttgttttagc cattacatta
    12781 gccgtatatc gaacatgggg ctggggaatc acccaatact caagtttgat ttatctaata
    12841 ttagccattt atggcgtcgt tgttcttttg caagcgatcc taggacgtga gccaaagaaa
    12901 gcggcagctt aaatgatcga gatggaactg gacacaggaa attttctgtt ctagttcttt
    12961 ttttattgta ttacatacat ttttgtgtat aattatacta tcgtttgcaa tggatagatg
    13021 attcacattt tgattgtttt ttcggtaaaa gtaaagattt ctatatttat cagtattttt
    13081 tcaaaaacca gcagttatag attgacaaag cctctcgagt atcgtaaact ttttttgtat
    13141 aaataactga atacgagatg cctcaatcaa tgaggtagag gtcgcgagaa tcattaaccc
    13201 tgtggagtga agcaaacatg gagaagcagg aatagggagg atcgccgaaa tgtgcacttt
    13261 tttgcttgga gtgtatgttg ggacgcaagt taagagcttg cggactgtct tagcagtgat
    13321 gctaagttgc gctatgtttt gtgagaggtt tatcacctta tggaatttta gcggcctttt
    13381 tatatctata aatcggtcgc tttttatttt ttcagataaa gctttaccgc atttcatcta
    13441 gaccgctcgt gtatcatata atcagaaagg gagaaaaaaa tgaaaaaaaa tatggtttca
    13501 gttgccgtac tatttgcttt tgtcttctca ctattcagtg tgggatctct ggcaaatgca
    13561 gatgaaaaaa caccgagcga tgagtttcgc gtaggtatgg aggctgggta tgcacccttc
    13621 aactggtctc aacaaacaga tgagaataat gccttgccaa ttcaaggcca aaatagttac
    13681 gccggcggtt atgatgttca aatcgctaaa aaagtagcag atggtttagg gaaaaaatta
    13741 gtcatcgtcc aaacgaaatg ggatggtctg gctcctgccc ttcaatcagg caagatcgac
    13801 gcgatcatcg ccggaatgag ccctactgct gaacgacgaa aagaaattga ttttaccgat
    13861 ccttattacg aatcacaatt agtagtagta gttcaaaaag atagcaaata tgcaaaagca
    13921 aaaagtttag cagatctttc tggagcaaag atcactgctc agttaaatac attccactat
    13981 actgtgatcg atcagatacc agatgtgcag aaacaacaag caatggataa tttctcagca
    14041 atgagaacag ctttagcttc tggaatgatc gatggttatg tcagcgaacg tccggaaggt
    14101 gtaacggcaa ctagtgtgaa caaagacttg gaaatgcttg aattcagcgg tgcagatggc
    14161 ttccagacaa acccggaaga tgttcagatt tctgttggta tgcgtaaagg cgacccggat
    14221 atccaaaaag taaaccagat tctttctggc atctctaacg atgaacgaac caaaattatg
    14281 gaccaagcag tcaaagatca gcctgctgca actgatagtg aagaaaaacc tggcatcatt
    14341 tctgatttca ctacgatttg gaaacaatac ggcaatatgt tcttacgagg tgctggactt
    14401 accttgttca tcgcattgat cgggacagtt gtcggtacta cacttggatt actgattggt
    14461 gtcttccgtt cggtacctga atcaggaaat gcactgactc gcttcttcca aaaaatcgga
    14521 aatgcacttc tttctatcta tgtcgaagta ttccgcggaa caccgatgat ggtacaagca
    14581 atggtcatct tctacggtct ggcactcgct tttggcattt cattgaaccg aacgattgcc
    14641 gctttgttta ttgtctctct caatactgga gcttacatga cagagatcgt tcgtgggggg
    14701 atttttgcag tagatgaagg gcagttcgaa gctgcgcaag ccattggtat gacccatggc
    14761 caaacgatga gaaaagttgt tattccacaa gttttacgga atatcttgcc agcaacagga
    14821 aatgagtttg ttatcaatat caaagataca gcagttttaa gtgtaatcgg cgtagctgat
    14881 ttgttcttcc aaggaaactc tgcttccggt gcgaatttcc aattcttcca aacatttacg
    14941 atcgtcggga tcatctactt agtcatgacc tttacgatca ccagaatcct gcgggcggta
    15001 gaaaagaaaa tcgatggtcc ttcagcttat accaagcttg aagaaatcga caattcaaat
    15061 ttgcaggaat aaggaggaag aatcatgaat gaaaaaatta ttgaaatcaa acatctaaga
    15121 aaaagcttcg gtgaaaatga agtattgaaa gatatcagcg taacagtcaa taaaggtgaa
    15181 gttgtgacga tcatcggttc ttccggttct ggtaaatcta ctttcttgcg ctgtatcaat
    15241 ttactggaaa aaccaacagg cggcgagatc atttataatg gagaaaatgt attagctcct
    15301 aaatataatt tacctaaata tcgaacgaat ctaggtatgg ttttccaatc cttcaacctg
    15361 ttcaacaata tgaatgtctt ggaaaactgt atgtctggtc aaacaactgt cctgaaacga
    15421 gataaagaaa ctgccaaaaa agtagcaatc gaaaatctgg aaaaagttgg tatggatcgt
    15481 tatatcgaag ccaaaccagc acaattatct ggtggacaga aacagcgtgt cgcaattgct
    15541 cgggcattat caatgaatcc agatgtattg ctattcgacg aacctacttc ggcgttagac
    15601 cctgaaatgg ttggcgaagt gcttaatacg atcaaagact tagcacatac tggcttgaca
    15661 atgcttatcg tgacgcatga gatggaattt gcaaaagatg tatctgaccg tgtcattttt
    15721 atggataaag gtgtgattgc agaagaaggt acaccagaag atatctttgt ccatccgaaa
    15781 gaagaacgta caaaagaatt cctttcacgt attttaaatg cttaggatca atagctgatt
    15841 gggacaaaga gctgcggtgt ttaccgcagt tcttttttat gaattactca taaaaggtaa
    15901 atttctgaaa attttggaat cttagttgtg tttttcagaa aactctctta atatggaagt
    15961 aattaaactt ttttagttag gatgatttaa aaatgaatga agagcaagaa ctgcagcgtg
    16021 gactgaaaaa tagacatgtc caactgattt ctattggcgg agcgattgga acggggttgt
    16081 ttttaggttc cggaaagtct atccagctag ctggcccttc gatattgctc gcctatctta
    16141 ttactggatg catctgtttt ttcattatgc gtgcgttagg cgaacttctg ttatcaaaca
    16201 ccaataacca ttcttttctg gattttgttg cagaatatct tggcaaaaaa gcggctttca
    16261 tcactggctg gacgtattgg ttctgctggg tatcgattgc aatggccgat ttaacagcca
    16321 tcggaatgta tgtacgctac tgggcgcctg gtgttcctca gtggcttcct gaactgattg
    16381 cattaggtct tcttctttgc ttgaatatgg tggctgtctc attgtttggt gagttagagt
    16441 tttggtttgc attgatcaaa gttgtagcga tcattgcctt tatcctcgta ggagcttata
    16501 tgatcctcac ccactatacg acagatattg gcacagccag tatcacgaat ctatggtctc
    16561 atggcggatt ctttccaact ggtgcaaaag gatttatctt agctttccaa atggtaacct
    16621 ttgcttttgc tgggatagaa ttagttggat tagtcgctgg agaaacagaa aatcctgaaa
    16681 aagtgttgcc ggaagctatc aataatattc ctatcaggat catcttattt tacttaggat
    16741 ctctttttgt cattatggca atctatccat ggaacagctt gaatccagat aacagtccat
    16801 ttgtagaagt attttcggaa attgggatca cgattgctgc ttccttgatc aatttagtcg
    16861 tattaagtgc agcagcttct gcctgcaaca gtgcaatata tagtacagga cgtatgcttc
    16921 gttctttagc gcaagaaggc agtgccccta aaaaattcaa aaagttaaca acacatcatg
    16981 tgccagggaa tgctttgaca ttttctacta tcgtcatttt catttctgtt atcttgaatt
    17041 acgtgatgcc tagtgaagta ttcacactag taagcagtat tgccactact tgtttcttgt
    17101 ttatctggag tatgcttgtc tacacgcaca tgaaatatcg taaaagtatc ctcggcaaaa
    17161 aagctcattc atttaaaatg ccgttgtacc catttagtaa ttatctggta tttctttaca
    17221 tggcctttgt gtgtgtcgtc ttgttcctag gaaaagatac gagaatcgct ttattactaa
    17281 cacctgtgtg gttcttgctt ctattactaa tctatcatat gaaatatgaa cgtaagaagt
    17341 aagaaagtag ttgtgacaga agtggtcagc ttcgagaaat aagaaggaat ttacgaaaat
    17401 tgcttcttaa atttttgtga aatttcggct tatttcttgg agataaaagc ttctgtccca
    17461 gcctcttttc tatttccccg gaaacaaccg ctgataatag gcatctgctt ttttatagga
    17521 aaaaatccaa ataccagata caaaaaaacg tccgaataac ccgatcaacg gtacaaaaaa
    17581 gacaccggca atcaagcata agttaatcat catggagata agacttcgac ggtcctttga
    17641 caaaactctc acgacatggg aatctggttt atttgctttc atgatttgcc tcatcaagat
    17701 accaaaagaa aaactccaca tcgtgtatac taagacataa gccatctcag gccaaaaagc
    17761 ttcagggtta cgcccgaccc aactagtaac agaaggacat aaagtcagcc aaaagagcaa
    17821 aaagatattc gaccagacca ctcggtaatt aattcgttct gccatctgaa atatacgatg
    17881 atggttcacc cagatcaccg caacaaatat aaagctgatg gtataagcta ataatgtatt
    17941 tcggatgacc cataaagaat gaaagctaga tgtattaggg atcttgatct ctaatacaat
    18001 aactctcaaa acaatggcta ccactccatc agtaaatgct tctaatctcg actttgtcac
    18061 gatgattcct cctcagatga ttcagcgctt ttattttatc acttacgtta ttaaataacc
    18121 atcgttttat atagggaatt ttctgaaaat tgttgcatat ttttcgaaat tttgttactt
    18181 ttaaaaaa
//
LOCUS       DAZAOG010000044        18165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000044 DAZAOG010000000
VERSION     DAZAOG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.044"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACHDLD_001268"
     CDS             <1..152
                     /locus_tag="ACHDLD_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3362984.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACHDLD_001269"
     CDS             complement(230..763)
                     /locus_tag="ACHDLD_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN3362985.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHDLD_001270"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHDLD_001270"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN3362986.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHDLD_001271"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHDLD_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN3362987.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHDLD_001272"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHDLD_001272"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN3362988.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACHDLD_001273"
     CDS             complement(4488..5066)
                     /locus_tag="ACHDLD_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN3362989.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACHDLD_001274"
     CDS             5388..5858
                     /locus_tag="ACHDLD_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN3362990.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACHDLD_001275"
     CDS             complement(5966..7192)
                     /locus_tag="ACHDLD_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3362991.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACHDLD_001276"
     CDS             complement(7946..8146)
                     /locus_tag="ACHDLD_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3362992.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACHDLD_001277"
     CDS             complement(8280..8888)
                     /locus_tag="ACHDLD_001277"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN3362993.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACHDLD_001278"
     CDS             complement(8973..9443)
                     /locus_tag="ACHDLD_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN3362994.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACHDLD_001279"
     CDS             complement(9491..10405)
                     /locus_tag="ACHDLD_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3362995.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACHDLD_001280"
     CDS             10551..11354
                     /locus_tag="ACHDLD_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN3362996.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACHDLD_001281"
     CDS             11532..12479
                     /locus_tag="ACHDLD_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN3362997.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACHDLD_001282"
     CDS             complement(12547..12840)
                     /locus_tag="ACHDLD_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN3362998.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACHDLD_001283"
     CDS             complement(12868..13533)
                     /locus_tag="ACHDLD_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN3362999.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACHDLD_001284"
     CDS             complement(13673..14305)
                     /locus_tag="ACHDLD_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3363000.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHDLD_001285"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHDLD_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN3363001.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHDLD_001286"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHDLD_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN3363002.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHDLD_001287"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHDLD_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN3363003.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHDLD_001288"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHDLD_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN3363004.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5390 a         3684 c         2905 g         6186 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgta
//
LOCUS       DAZAOG010000045        17737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000045 DAZAOG010000000
VERSION     DAZAOG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.045"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..850
                     /locus_tag="ACHDLD_001289"
     CDS             38..850
                     /locus_tag="ACHDLD_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN3363005.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            847..1647
                     /locus_tag="ACHDLD_001290"
     CDS             847..1647
                     /locus_tag="ACHDLD_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN3363006.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1808..3076
                     /gene="rseP"
                     /locus_tag="ACHDLD_001291"
     CDS             1808..3076
                     /gene="rseP"
                     /locus_tag="ACHDLD_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN3363007.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3144..4853
                     /locus_tag="ACHDLD_001292"
     CDS             3144..4853
                     /locus_tag="ACHDLD_001292"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN3363008.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5065..9423
                     /locus_tag="ACHDLD_001293"
     CDS             5065..9423
                     /locus_tag="ACHDLD_001293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN3363009.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9568..10041
                     /gene="rimP"
                     /locus_tag="ACHDLD_001294"
     CDS             9568..10041
                     /gene="rimP"
                     /locus_tag="ACHDLD_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN3363010.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10064..11239
                     /gene="nusA"
                     /locus_tag="ACHDLD_001295"
     CDS             10064..11239
                     /gene="nusA"
                     /locus_tag="ACHDLD_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN3363011.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11261..11554
                     /locus_tag="ACHDLD_001296"
     CDS             11261..11554
                     /locus_tag="ACHDLD_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN3363012.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11551..11862
                     /locus_tag="ACHDLD_001297"
     CDS             11551..11862
                     /locus_tag="ACHDLD_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN3363013.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11875..14181
                     /gene="infB"
                     /locus_tag="ACHDLD_001298"
     CDS             11875..14181
                     /gene="infB"
                     /locus_tag="ACHDLD_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN3363014.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14208..14555
                     /gene="rbfA"
                     /locus_tag="ACHDLD_001299"
     CDS             14208..14555
                     /gene="rbfA"
                     /locus_tag="ACHDLD_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN3363015.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14712..15168)
                     /locus_tag="ACHDLD_001300"
                     /pseudo
     CDS             complement(14712..15168)
                     /locus_tag="ACHDLD_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15333..17695
                     /locus_tag="ACHDLD_001301"
                     /pseudo
     CDS             15333..17695
                     /locus_tag="ACHDLD_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
BASE COUNT         6044 a         3154 c         3904 g         4635 t
ORIGIN      
        1 agatagtggg aatcttttaa gtataggagg aaaaagaatg ttacgttttt ttccgcaaaa
       61 aaacaaatat attcaagaaa aaagcgaatt tcaatttaat accgatggac caattcctca
      121 gcacattgcc atcatcatgg acggcaatgg tcgttgggcg caaaaccgtc gtttgccacg
      181 agttgctggc cataaagaag gcatggaaac agtaaagaaa gtcacaaaaa aagcatcaag
      241 gcttggagtg aaagtgttga cgctttatgc cttttcgact gaaaactgga agcgaccaaa
      301 agatgaagta agttttctca tgcaactacc tgtcgacttc tttgatacgt tcgtaccaga
      361 attgattaaa gaaaatgtca aagttcatgt catgggttat gaaaacgtat tgccagaaca
      421 tacgcaagat gctgtaagac gtgcaattga gcaaacaaaa aacaatactg gaatggtatt
      481 gaattttgct ttgaattatg gaagcagagc agagatcgtg actgctgtcc aagaaattgc
      541 cgaagaagta gcaaaaggag aaatacacgc agaagaaatc gatgatgaat tgattgctaa
      601 gcatttaatg acaggtttct tgcctaaaga gttacaagat cctgagctga tgatccgtac
      661 aagtggagaa gagcgaatta gtaattttct gttatggcag atcgcatata gtgagctgta
      721 ttttacaaaa gcgctttggc ctgattttga tggtgcgcat cttgaagaag caatcgcctc
      781 ttatcaaaat cgagaccgtc gattcggcgg tgtaaaaaaa acagaagatg agaaagggga
      841 tcagtcatga aacaacgtgt ggtcacagca gccgtggcgc tagtcttgtt tattccgatt
      901 atatggatcg gaggcatagc ggttgagctt acagcagcaa tcttagcagt aataggagtt
      961 tacgagctgt ttcggatgaa aggattgaca ctcttaagtt ttgaaggagt tttatccgcc
     1021 gttggtgcag tgttacttgt tttgccaaaa gaaagatggt ttttctttct tccagagaat
     1081 gtaagtacat ttatgttatt ttatcttata gttatgattc ttttaggaac atcagttatt
     1141 tctaaaaata cgtatacgat tgatgaagca ggttttcctg tgcttgtcag cttgtatgtg
     1201 ggaataggtt tccagaattt tgtcaatgca cgaacagaaa gcttggctgt tttgatattt
     1261 ggtctattta ttgtttgggc aactgatatc ggagcatata tggttggtcg acgttatggt
     1321 cagcataagt taagtcctga tatttcgccc aacaaaacga ttgaaggtgc gctcggtggt
     1381 attggtagtg ctgttgtagt cgcatttctt tatttattgc tatacccagc aaaagaacta
     1441 tttaatcatg gtatgtttgt catgcttctg atgacgatta ttttttcagc agtcggacaa
     1501 tttggtgatt tggtcgaatc tgcaatcaaa cgtcattatg atgtgaaaga ctcgggaaat
     1561 atcttaccag gccatggtgg aatcttagac cgtttcgaca gtttgctgtt tgtatttcct
     1621 atcatgcatt tatttggcat tatttagtaa gtatacctgc gtttctttgt gaatttcata
     1681 aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg gtatactatg
     1741 gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag taaaggagaa
     1801 aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag tgattgttca
     1861 tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg aatttgctat
     1921 cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat atacgcttcg
     1981 cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg aaacagaaat
     2041 ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag agaagataaa
     2101 tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc gttatgattt
     2161 agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag tagtaaccta
     2221 ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca gaattgcgcc
     2281 aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca actttgctgg
     2341 acccatgaat aactttattt tggcaatcct gttattcatc attctggcat ttatgcaagg
     2401 tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg gagctgcggc
     2461 agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag aaatccattc
     2521 atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag actttaagat
     2581 cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg
     2641 cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta tggataagat
     2701 cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag ccttaggatc
     2761 actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt accaattgtc
     2821 ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc ttctttcaat
     2881 gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga aattagtctt
     2941 gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag gaatattgac
     3001 attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga acgatattca
     3061 acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga taactatttt
     3121 gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca acattacgcg
     3181 aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct ggatatatcc
     3241 gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta ttggaaaaac
     3301 tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg
     3361 ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat ggaccaaact
     3421 tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact cacgaagaaa
     3481 cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct ttaaatcttt
     3541 atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa
     3601 gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt ttggacgaga
     3661 catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc ttagaatttc
     3721 gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa ttcatggcga
     3781 tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat gctgcaaact
     3841 tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg
     3901 agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg
     3961 aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct gtccttgttt
     4021 tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta tctgctgact
     4081 ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag tttggttctg
     4141 tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat gtccaagatt
     4201 tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat gtgaatccag
     4261 aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa ggagatcctt
     4321 caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga catatcttca
     4381 aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa aatggacgag
     4441 aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta tcagcaatcg
     4501 tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg ccatttgatt
     4561 tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg aatgaactag
     4621 aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat gaacgcgcag
     4681 gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact gtcggtaaaa
     4741 aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac
     4801 gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa taataagcgt
     4861 taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga tgattcgctt
     4921 ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa
     4981 aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt
     5041 tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga aaaactgttg
     5101 gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc tggtgaaatg
     5161 gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca tttttccaaa
     5221 attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc ttttaaagat
     5281 attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga acagctcttg
     5341 caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact agcacagcaa
     5401 gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt
     5461 acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga gttgttcgtc
     5521 agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca agcggaacga
     5581 gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat gaagcaagca
     5641 gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct
     5701 cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga accaacgaca
     5761 ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta cgtattcgac
     5821 aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat cacagattat
     5881 acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca agtatttgat
     5941 gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga tacgtttgtc
     6001 cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc tcgcaaagat
     6061 tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat gagtacgatg
     6121 gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg acaccgagct
     6181 atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag tgctggaaaa
     6241 aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga tggtgtccca
     6301 attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt atttgacgtg
     6361 gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc agtcaaaatg
     6421 tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca tcctttgtca
     6481 cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc aaagtcggaa
     6541 gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt agcccacaac
     6601 gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat tcctgaagcg
     6661 gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca attcaaacgt
     6721 ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca tcaccgagca
     6781 atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga ggcaatggac
     6841 aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg agattcttac
     6901 aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg actgaaagat
     6961 ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc acgaatacca
     7021 cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg tgataaggga
     7081 gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg agcaaaattc
     7141 tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga gcaagaattg
     7201 gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat cggaaaaagt
     7261 ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga agatgccatt
     7321 taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg tcatagtttg
     7381 ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt tttaggggaa
     7441 gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat ctgcgaagaa
     7501 gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga agatgaaata
     7561 tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc tgagatcatt
     7621 caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc ggttatttat
     7681 ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt aggttcacgt
     7741 ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt taatccgtta
     7801 gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga tggtacgtat
     7861 ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg cttaaacaaa
     7921 gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa agtgcccgat
     7981 attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac gaaagtattg
     8041 tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga caaaacagca
     8101 tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag tgcagaagtc
     8161 gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca tccagggggg
     8221 atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca atatccagca
     8281 gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat tcacgataat
     8341 gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat gcttcaagat
     8401 ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat gcggatattt
     8461 gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac agggacatta
     8521 ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac gcatcctaca
     8581 acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt atggttagga
     8641 aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat cggctgtcgt
     8701 gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc atttaaaatc
     8761 atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg agaaacctat
     8821 ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg ttcaaaaatc
     8881 aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct tcgtgtcgct
     8941 tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt acgtgcagac
     9001 gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc aatgaaagaa
     9061 atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac agtgctggag
     9121 ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt gtataaatcg
     9181 gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag agctgtccca
     9241 agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg tccattttta
     9301 tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga atatatgaac
     9361 gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt cgatatgcta
     9421 taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata gttcttatag
     9481 gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat ggaattacgc
     9541 acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg
     9601 gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa
     9661 ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa
     9721 tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt
     9781 cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa
     9841 agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat
     9901 ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa
     9961 atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct
    10021 cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta aagaaatgtt
    10081 aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg tgatcgatgc
    10141 tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac aaaacgtcga
    10201 agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag aagtaacgga
    10261 agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa tcaatccagc
    10321 atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg ggcggatcgc
    10381 tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa gaacgattat
    10441 ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg aacgccagga
    10501 taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta aacaagatca
    10561 aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt cccgagtaga
    10621 aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc tattgcgccg
    10681 cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg tcagcatcgc
    10741 ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa atatcgatgc
    10801 agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca atgaattaaa
    10861 aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca ttgcaaatgc
    10921 attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa aggcctgcac
    10981 agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac aaaacgcacg
    11041 tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg acatgacgga
    11101 attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg aagcaatcgt
    11161 tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg aaacggtcga
    11221 agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa ggaaaattcc
    11281 tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg tccgaatcac
    11341 ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg gacgaggagc
    11401 gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa ttctcgatcg
    11461 agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact atgtcactca
    11521 ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca aaaagccatg
    11581 aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga actgaccatt
    11641 gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc aagcgagaat
    11701 acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt tgagctattt
    11761 tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg aataatggat
    11821 gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga ttgcatgggc
    11881 aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga cgtcgtggaa
    11941 aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac tagcaatgat
    12001 gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc tgcacaacaa
    12061 gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga aacaacgaac
    12121 aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg gagcggtcaa
    12181 gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac cagcagccaa
    12241 aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa caacaaacca
    12301 aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa taaaaaaggt
    12361 aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa attccgtgaa
    12421 ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc gaaaaaaatt
    12481 catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat ggtcaatcaa
    12541 aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg catggaacca
    12601 caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc agaagaagtc
    12661 gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca cgtagaccat
    12721 gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg cgaagccggc
    12781 ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc aatcactttc
    12841 ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc aagtatcaca
    12901 gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac agttgaagca
    12961 atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa aattgataaa
    13021 ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt aattcctgaa
    13081 gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca aaacatcgaa
    13141 gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc agatccaaca
    13201 caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg tcctgtgtca
    13261 accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt cggtaatact
    13321 tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga agcaggacct
    13381 gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga ccgtttcgta
    13441 gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa acgtgcatta
    13501 ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga aagcctgaaa
    13561 gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg ttcagcagaa
    13621 gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa aatcgtccat
    13681 tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag caacgctatt
    13741 atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga tactgaagaa
    13801 gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga aactgcgatg
    13861 aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat cgttcgtgaa
    13921 accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga aggatatatt
    13981 cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga aggccaactt
    14041 gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta tgaatgtggt
    14101 gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg atttatcatg
    14161 gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg gctaactacc
    14221 gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg cgcaaaaagg
    14281 tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg ggggacttac
    14341 agcaagcaac gatctattac agcattttat ctgacttagc atcagataag aaaacagcac
    14401 aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac aatctaagta
    14461 tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat ggcaaccata
    14521 tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca aatcctgtga
    14581 aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag agttccatga
    14641 ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta aaaaattcga
    14701 ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa gataccgata
    14761 acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga
    14821 tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa
    14881 atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat
    14941 tttggataat ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa
    15001 agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct cgatccagcc
    15061 ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat gcatgatgag
    15121 gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa
    15181 aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat
    15241 aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag
    15301 aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg
    15361 ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta
    15421 aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa catccaatcg
    15481 gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca
    15541 ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga caagtcatgg
    15601 tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg
    15661 agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt gcttctcatg
    15721 cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac
    15781 atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg
    15841 gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag
    15901 tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa
    15961 cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg
    16021 aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg gctgaaaaaa
    16081 tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg aagacagcaa
    16141 cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg
    16201 gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga
    16261 ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct
    16321 atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaatcg
    16381 cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa
    16441 gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa ccaggctccg
    16501 ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc
    16561 cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt
    16621 tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt
    16681 cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag
    16741 aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat tcttgcgagt
    16801 ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg acctttcttg
    16861 gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc agcaagatca
    16921 caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa agaaggcgga
    16981 gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca tggataaagc
    17041 attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc gtgctcaatt
    17101 ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca ttgattgggc
    17161 ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa cggaatctaa
    17221 cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac taaaaattcg
    17281 gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc cgcgtgggtt
    17341 gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat ttgctttcca
    17401 cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc atttaatgat
    17461 tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg tagtaaatga
    17521 acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa gtcaagcaga
    17581 agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata ttcgtgaaaa
    17641 tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc aataaaatga
    17701 taaaatcgat cccgcaaaat agaatttttt acattga
//
LOCUS       DAZAOG010000046        17242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000046 DAZAOG010000000
VERSION     DAZAOG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.046"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..473
                     /locus_tag="ACHDLD_001302"
     CDS             36..473
                     /locus_tag="ACHDLD_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363016.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            568..1452
                     /locus_tag="ACHDLD_001303"
     CDS             568..1452
                     /locus_tag="ACHDLD_001303"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3363017.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1676..4842
                     /locus_tag="ACHDLD_001304"
                     /pseudo
     CDS             1676..4842
                     /locus_tag="ACHDLD_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            4910..5407
                     /locus_tag="ACHDLD_001305"
     CDS             4910..5407
                     /locus_tag="ACHDLD_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN3363018.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5426..5890
                     /locus_tag="ACHDLD_001306"
                     /pseudo
     CDS             5426..5890
                     /locus_tag="ACHDLD_001306"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5887..6645
                     /locus_tag="ACHDLD_001307"
     CDS             5887..6645
                     /locus_tag="ACHDLD_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3363019.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6731..7471)
                     /locus_tag="ACHDLD_001308"
     CDS             complement(6731..7471)
                     /locus_tag="ACHDLD_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3363020.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7532..8785
                     /locus_tag="ACHDLD_001309"
     CDS             7532..8785
                     /locus_tag="ACHDLD_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3363021.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9107..9334
                     /locus_tag="ACHDLD_001310"
     CDS             9107..9334
                     /locus_tag="ACHDLD_001310"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN3363022.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9457..9783
                     /locus_tag="ACHDLD_001311"
     CDS             9457..9783
                     /locus_tag="ACHDLD_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN3363023.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9851..10564
                     /locus_tag="ACHDLD_001312"
     CDS             9851..10564
                     /locus_tag="ACHDLD_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN3363024.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10874..11725
                     /gene="licT"
                     /locus_tag="ACHDLD_001313"
     CDS             10874..11725
                     /gene="licT"
                     /locus_tag="ACHDLD_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN3363025.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11846..13711
                     /locus_tag="ACHDLD_001314"
     CDS             11846..13711
                     /locus_tag="ACHDLD_001314"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3363026.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13725..15168
                     /locus_tag="ACHDLD_001315"
                     /pseudo
     CDS             13725..15168
                     /locus_tag="ACHDLD_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15276..15977
                     /locus_tag="ACHDLD_001316"
     CDS             15276..15977
                     /locus_tag="ACHDLD_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3363027.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16103..16615
                     /locus_tag="ACHDLD_001317"
                     /pseudo
     CDS             16103..16615
                     /locus_tag="ACHDLD_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17013..17222)
                     /locus_tag="ACHDLD_001318"
     CDS             complement(17013..17222)
                     /locus_tag="ACHDLD_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN3363028.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5757 a         2897 c         3713 g         4875 t
ORIGIN      
        1 aaacaatatc aaaaaatgca cgtagaagta gataaatgga gtggttttgc aaatttcttt
       61 tatttctaga agttattcac gtatacggat ttgtttttct cacaaataaa aaggcaggaa
      121 gtacaatgga tcatttgtat ataaaatata ttcatcaatt ttccgttttg gtgttaggca
      181 cactaattat agcggggatt gtcgcattta ttattcaagg ccgtgacata ttaacagtac
      241 atcaagagtt tttcagttat gcatttggta ttcctctgat cgctataagg tctgataaca
      301 aaaagtggaa gaaacgctat tacattctct tgctattttt gtgtttttat ctgccaccta
      361 agatttttac tttattaggt atacaatgcc tatcattttc ttttatttcc tatatcattg
      421 ttttaatttt cctcagtttc atagtatgga tgttttatct tgtcaaaaaa taaatgaatg
      481 tttcatgaac agtacttcat cacagcctaa tttttttctt tatgctatcg ttatattaaa
      541 gaaatcaatc agaagggaaa gaaagtgatg aaaaaagttt tattagtggt aaaccccagt
      601 tcaggcggtg aacaagcaaa ggaatttgag caattagcga tagcaaaatt ggaatcggtg
      661 tttgatgaag tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa ctttactcgc
      721 gaagcggcta cggaagggta tcatagtgtg tttgtcatgg gcggagatgg aacagtcaac
      781 gaaggaatca gtggaatcgc tgaacaagaa catcggccaa atttcggatt tttcccattg
      841 ggtacggtga atgacttggc tcgagcatta gggataccgc tagaacctga ggaagcgatc
      901 aatcattttt cgattgaatc agtcaagcca ttggatattg gaaaaataaa tgacgactat
      961 tttatgaatg tcgtcgcaat tggctctatc cctgaatcaa tcaatgatgt agatgcggag
     1021 aagaagacga aatttggaaa actagcttat ttcatgtcag gaatcaaaca gttagcttcc
     1081 acacaatcct actcgtttca cgtagaagtt gatggaaaga aggaagaaat tgagagcagt
     1141 accttgctga taggactaac taactcagtc ggcggttttg aaacattact tccaaatgca
     1201 aaagtggatg atggtaagct ccatttggtt tatttaaaag attcttcttt gatcgatacg
     1261 cttaaaacat tgccagacct attaaaagga gtagatgaat ctacgaaaaa tctagcctac
     1321 cttacttgtg agcaattagt tgtgtcatta gctgatgagg aagaattgac gacgaatgtt
     1381 gatggagatg aaggggataa attacctgtg acaatcaaag tattgccctc acatttacag
     1441 gtttattgtt aaaatggagg ttgtgaccga ggagttggcg ggagaacacc gcttgttcgg
     1501 gataagggac tgtgacaaga cttttgtcac agcctttttt ttgtacaaaa aagaaattta
     1561 cacctaatag aaaaattttt ccataaaaat acatatttta aatatgaaag tacaattgga
     1621 aatagattaa aacgataaaa taaaattgtc aaaacgtcag agaggatggt ggagaatgaa
     1681 tcaatgtgta aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac
     1741 atccgttttt gccgatgtag agaaaacaaa cgaatcaacg caatcagaga atcaaagttt
     1801 tgacaagact actttttcag aagaatcgat tacttctgaa actacagaat ctcaaagtga
     1861 ggcttctact aataaaaggc aagaagaaaa taagacaagt gagacctcag aagtagctga
     1921 acaaacgtct gatagcagtg aagcggcact ggcaagagct gtttcagcgg atgggtacag
     1981 cgaagctgcc acaacagaag aactggcgca gcttctcgca gacgaatcag taactaagat
     2041 ccgactgatt cagcctttga ctttagacag ggaattagag atcaaacgag atatcgttat
     2101 tgattttggt ggattcgctc acaatttcgg cacacaccat atttatatca atgagatacc
     2161 tcagtcgatc gagttccaga attttaaagg aacagctgca catccaggtg gattgacacc
     2221 aaatatcgat ggaaatgcca ttatcattgc ttatatgacc gatttttggg gaagcagata
     2281 tcattttacg ggagaaataa aatttaccgg gacacttgat ttgtatgatg ggtccaaatt
     2341 aggactgatt tacgctcctc gtgcaactgt cacattagat ggtgtttccg gtgtgttgga
     2401 tgtccaacca gtgaaggaag gactgaatgc cgctccgggt aaagcctatt tttgtagaag
     2461 ctatcaattg aatgtgatca atggtagtca actttatgga ccgtatctag ggaaattcta
     2521 cggttttcta gattcaggag atgagactgg tagtggaaaa acagcgccag gaatccatat
     2581 cttaagcggt tcaaaggtct ctcttgatta tgatcgggca gatggcgtta gcgatgaagg
     2641 agaggcagtc gatacgctac ctggtaatgt catgtttgaa gtcagtggtt cgggaagcga
     2701 attttcggta aatacaaaga tcaatattac caatgacaac aatcgcggaa tcatccaaat
     2761 gagaggttca ggatcgaaag tcactgtatc gaatggtggg aaaatcacca tcaatacagc
     2821 aactacgggc ggatttcgct tgcaaggaga ttctagtcaa atccatgtat caagtggtgg
     2881 acaaatctcg gtcaaaatgg ctggagatga cgatcaaccc gggaacaatg ggatgcgttt
     2941 tgtaggaaaa ggcctttctt tgatcgtaga aggagccgga tcagttatcg acatcgataa
     3001 gcagagtggg cgttccagcg cagttcgttt tgagaatggt acgcaaaagc tgacagtaac
     3061 caatggaggc tcattgaggg taagaaatac aggtgatggc aaatcttacg tgaacagact
     3121 caatcaagga aatcaagcga tccaatttta tgacgcttct ggttttctag aaagtccagg
     3181 ttcagctgat tttacagtca ctggagaaaa atcaaatatc gatatacgcg cagattccgg
     3241 agcaactgta gatacaactg gagatattga tttaaatttt acggcaggtg aaaaaactta
     3301 tttagtcatg gtcgggaaaa caggggaaga taagaccggc atcatcagtg gggatgtgtt
     3361 gaatgtggac ttagaatctc ctacctattt tgatttccaa aacaaacgga ttggaagcga
     3421 gaccaatggc ataggcggct gggtattcca aggaaatgga gattcttctt taaagatcgc
     3481 ccattcagaa atcgctttat ggcgaaaaac aggcgcaaca ttcgataact ttgaaaaaga
     3541 cccggattat ttttctgata tgacagactt atcgatcaat gggaccgact taggcaattt
     3601 cgtatcaagc cctagtgcag gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa
     3661 aaattataat cgaatcagtg ccaataatca gctcccgatg attgaatcct taagagtgcc
     3721 aacgaatgca gataaaaaaa tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa
     3781 gccaagagat gcttggacag gcgaagtaga agtgacttta cgtattactt acgcagatac
     3841 gacgaaatca tctgtagaaa ttactgctct cacgcaagga caaacagatg aaaatgaagg
     3901 ggcaggatat aacccatggg gagaaggcga acaaggcggc ttgttcgaag ttcaagtacc
     3961 caatgatgag agattaaaaa cggaggatca agtgcaagtt gttgctgcta aaaaagtaag
     4021 aggtggccaa gaagtgacag aactggactc tgccaaaacg acagtcgatg tgattcctcc
     4081 tgaacctgct aatttaagta ccacattgat caatgcagct tctcgcacga tcacaggtac
     4141 aggtgaagca ggagccttgg tttcattgaa aaaaatcaag ctgttttgtc agaaacaact
     4201 attgatgata caggggaatt tgttctttct atcccagaaa atcaactttt tgcaggagat
     4261 gtattagaaa ttgtattaaa cgaccaagca ggagaagcta ctgctaaggg tgtgcagaac
     4321 aagccaagta ccaacgatga aattgggaat cacaatccaa gtgatacgcc agttgtttat
     4381 catgatgccc cgccttttgc acctgctacg aagatcgtag tagaaggtgg tctctcattt
     4441 cttgcccctt ccaaactcga ttttggtcag atcaaagcaa ctggtttgac acaagaagca
     4501 tttggccaaa tggatataca ggaaaaatta tggatttatg atcagcgaga aacgaaaaat
     4561 ccgtggatct taacgataaa attatctcag cctttcacaa atgatgcagg attttctatg
     4621 caggatgtcc tatatttcaa gaaaggagag gagtatcaat tagtgaatga agaagaactg
     4681 gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag attattcaga atatttaaat
     4741 aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc agatagctgg taattactca
     4801 gcagagataa catggacatt agcagatgcg ccgacagaat aaagatcaag gaggatgatt
     4861 gatgaatcga atatggaata aaagtcatcg aaacaactgc tctttactta tgatccttct
     4921 ccttggattc atcctttggc agacacctgt ccatgcctcc gaattcaatt ttgcggtaga
     4981 ttttgaatta ccggctaatc agattgataa gaacaaaaca tatataaatg taaggatgga
     5041 gcctgggaaa acacaggaaa tcccctacac ggtctttaac cgaacagata aatcagtaac
     5101 agttgagaca ttgatccact ccgctacgac gaattcaaac ggtgtgatcg aatatggaga
     5161 atcaaaaagt aaatttgatg aaacattaag ttatgatatg aaaaacttag caaaaacagt
     5221 gggagaaata actgttcctc ctcaaagtgc ggtcactgag acactttcac tgaaagcgcc
     5281 agaaaaagag ctggctggtg tggtcgcagg agggatcacg tttcaagaaa aaaaagagaa
     5341 cgtttctaac aaaagtacag ttggtgtcgt caatcagttt tctcaagtga aagcaatctt
     5401 gatttgaaat agcgaaaaag agtcttcccc agatttgaaa ttaaaaaaag cttatgcgga
     5461 tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa aatccaaatg cttcttatat
     5521 gttcggcttg actacatcgg ttgaaatcta caaaaaagaa acaaaagagc cttacctttc
     5581 aataaaaaaa gaaaaaatag atgtagcacc caatacaaat tttgatttac ttgtcccatt
     5641 agaaggaaaa aaattagctc cgggacatta tcaagcggtt gtgaagggtg cttggaaaga
     5701 aaaacgctgg aatctccata ccgattttga aataactgat gatgcagcgg accaattgaa
     5761 tgaaaaggat attgatttaa aaaatcagcc agaaacattt gactggcgat ggctgatact
     5821 tgttggagtc attcttattg gcttatttgc tggattattt attttaaaag aaagaaggaa
     5881 aaagaaatga aaaaaacaat tgcattaagt accgttctag ctggaagtct attattatgt
     5941 acacaaagtg tttttgctga gaataagagc tcagccacta cagaaggaac gatttctgtg
     6001 accaaaccag atgctgcaaa tcctgaagac gttgttccag ttcctcctgt tgatccaaat
     6061 gatcctgata ctccttttac acctgacgaa ccaaatcagg gaacatccgg attactgacg
     6121 attgaccaag ctccagatct tgattttgga acaatcaagt taggtacagc aaaaacagta
     6181 tatgctgcat tacaaagcgg aacggacagt actggagcac aaacacaagt acagaatttc
     6241 gtacaagtaa cggataaaag cggaaattat gccggttgga aattatcagt caaacgaaca
     6301 gaatttattg gtacaacatc tgatacgaat aaactaactg gttcaaaggt gaaattcaaa
     6361 aacgccgtga ttcgaaatag cgcaactaat agcagtggaa tgccatctac cgtttctgga
     6421 actggcgctg aaggaatcga aatcccagca gatcaggcag tagatctagt aactgcagga
     6481 gcaaatgaag ggattggcac atggatttat tcattaggag agaatgtgga acaagggaga
     6541 caatcagtgg aattatttgt tcctgtatcc aattatgctg tagacaacta cgtttcaaca
     6601 ttcacatggt cgttaacaga tgctcctaca gaagaggaag cgtaaaatca tatcaacgat
     6661 ttcagggagt ggagccgatt caaggcccac tccctgaaat ctgctatgct tacgtctcag
     6721 ctcttattca tcaactcgaa gcagtttttt ggaagtcatg gaaacgttcc tttgtctttt
     6781 caaagatctt tcgaccaacg agtaaggagt caccgttgaa aaattctaac acataatgag
     6841 cagaatcaat tttctgcaat tgcgataagt tgatacagta agattttaaa agtaaaaagt
     6901 aagcatactt ttctaactct gtcttcaatt gttttaaaga agtattcact aatttatctt
     6961 ctgtagttgt tttgaccata acttggtttt tcaaaccttt gatactttca atatataaaa
     7021 tttcccgagg atacaggacg agacttccac ctttttgatc aataaagatt ttatcgacat
     7081 ttacgatctg ctgttctttc caattgatct gttctagcac ttgacgaatt tgttcagata
     7141 aattcctgtc ttttctgata tatccaatcg gaaggatttt ccgattgatg atctctactg
     7201 caaaattcgt ataagcggtg aaaaaaacaa tattactatg tgcatttttt ttacgtattt
     7261 gttcggcgac atcaatacct gtgagtgatt gcttcaagtc gatatcgata aagaaaacat
     7321 cgtgattatc aatagtgccc aactggatca actgattgat ttgagagata gaagtgagct
     7381 gcacgatatt ctcgatatga tgattgacca attctctgat gatccattcg ctgtgaaact
     7441 ggttatcttc tatgataaaa tactttttca tatgcaggct cctattaaaa aaatatgtgt
     7501 acattttgaa acaaaacaat ggaggaataa gatggtcgca acagtgacac tttatttgac
     7561 ctttactttg attttagatt taggtatcta tttattcttt tcgaaacaac tgctagtaaa
     7621 aaacagcttt ggttgtatag ctttgatggt ggtgacactt ttacttccta tccctaaagt
     7681 gagctttcct tacctagttg gcttattatt gatgtttctt ttttatttta ctacgaaaaa
     7741 cctgtttatc agctgtttga tcagtaatct tttttttgct atcactggac tcagcttttt
     7801 tctggttttt gatctgatgg attatttagg aaagagacat cttggaacag caagtttttt
     7861 tgcactactt atcgcattta ttttctatct attagtaaaa agtatagaca aacagacgag
     7921 atttctcaac ttgcttttta cttatccaac tgaacactat tttggaggta tttgtatttt
     7981 attaagctgg ggcgtgattt acttggtatt gggaagtgtt cacttccgct catctaatta
     8041 tctgcttcta tctcttttgt cgatagtcat cagttttttt tctttgtttt tttccattat
     8101 gttgatcagt ttccaagtaa aagaaagaca acatcaggaa tcgcttcaac tgtatcaaat
     8161 aaatgaagaa tattaccatc atctagagga attcaaacat gattataaaa gtctactgtt
     8221 ttcattaaaa attactcttc aggaaaataa aaaagaaacg ttggaatttt tagaaatatt
     8281 agaagatcat tctgcagcta tttttgaaaa accgcaaata caacagctaa caaatattgt
     8341 atccccagct gtcaggggtg tctttttaaa atttctagaa aaagcagaat cggagaaaat
     8401 cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc ccgattgatc tggttgtttt
     8461 tattcgctgt ttatctattt ttatttctaa cgcattcgat catcgtgtag ccgatcagag
     8521 tccgattacg atcaatctgc tggaagaaaa agagggatca cgttttagta tagcgaacaa
     8581 ggcaaatcca acgaatttga cgctatcgga gatggtcaaa agaggaaaaa cctctaaaaa
     8641 aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg gatgtttacg aaaatgcaga
     8701 actaaaaatc gaattttcaa aacagaatca ctattttttt gtagaaatgt atctggcatc
     8761 tagaaagagc aaatcagcct actgacttac tgtcaaagga taagtttgag gaaagtctac
     8821 acggagatag agacaacaac acagcatatt caaaagaaac agaagcgtga tgaccgttgt
     8881 cacccttctg tttcttttgc tatgtagtta aacaaagtag ttgacacttc cgctacttag
     8941 tattagtacg aaagtatact tcatgatact aagtagataa gaagtgaaaa gcggaaccca
     9001 cgatcgtcaa aatgctaact gctttgctta caccagataa agggaagagc aatatcgctt
     9061 attttaggcg tggagaagca agcaaatccc tacgatcaca aaaaaaatgc tgacccagca
     9121 gttaagagag ttagaacaag atgaaagtat ccatcgaaaa gcctataacc aagtcccgcc
     9181 taaagtagag tactcgctca ccgaagaagg aaaatcatta agagagattc tagtagcgtt
     9241 gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc tcaagggaag aagacacgct
     9301 actgcataca catggtggct acctaaatta ttgattttct atgacattta ttttaaataa
     9361 aagaaaacag gagttttttt aaaagcatca tctatttttt atgtaaaaaa atggtatgat
     9421 taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga aaaagtcaac
     9481 cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt ggctatctct
     9541 atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc ggaagaagta
     9601 aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat ctattcatac
     9661 atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa attgatcaaa
     9721 aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata cacagagttg
     9781 tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa tgagtctgga
     9841 gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata tcctcttgtt
     9901 cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg attattttgt
     9961 cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc tgaaaaagaa
    10021 taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga cagaaggggt
    10081 catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg cagtcccttc
    10141 agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac tttacggagc
    10201 aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag caagccatcg
    10261 tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg gtgccaagat
    10321 ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag aaaaagttgc
    10381 tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca cactaattac
    10441 ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg aaagtgttac
    10501 atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga caaaaacatt
    10561 ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata tgaatcgata
    10621 aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc tgatttggtt
    10681 gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag gcttgcataa
    10741 ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg tacggcaggc
    10801 aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat ttttcttgag
    10861 agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca tttcgaaaaa
    10921 tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa aaaagattgg
    10981 ggacgagatt tcgccagaag cggtccaaaa agaatttgtt ttaagagatt cttttgctaa
    11041 aaaccaattc cagcagctga tggctgatat tccggcagag gagatcgaat gggtcaaaca
    11101 agtagtcgaa ctggctgaag ataaactaaa agttgaattt tcgcctaata tttatctgac
    11161 attgaccgat catcttcatt atgcgatcac acgagcaaaa gaaaatatcc aactgcctaa
    11221 tccattatta tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag cgaaagaggc
    11281 tcttttgttc gtgaaacaaa agacgggtgt ggaactttct gaggatgaag ccggttttat
    11341 tgcctttcat tttgtgaata gccaacaagg aaacgaagac atgcaagtga cgatgaccgc
    11401 taccacaatg gtaagagata tactgagtat catcagtaaa ttcttcggca tcgtgttcga
    11461 tgaagaatct ttgaattatc agcgtatcat tactcatctg cagttttttg cccagcgtta
    11521 tctcaaaggg gaaggatctg acgagcaaga tgaatttctc tacgcattag ttcaaggaaa
    11581 gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac aaacgcaaaa
    11641 acaggaaatg gggatcgcag agcagattta tttaacgatt catattcaaa gagtagtaag
    11701 tgagaaaaaa agcatccaat cataaggatt gttactgtat ggcaggcaaa acctggattg
    11761 aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa tctgggtttt tattttttaa
    11821 ataaaaaaga aagaaggaaa aaacaatgga taatcaagca gttggtcgtc gtgtatggga
    11881 agcggtagga ggacaaaaga atgtcaaaag tttagtacat tgtgctacgc ggttgcgatt
    11941 cagattaaaa gatgaatctt tagcagatac gcaaaagtta aaagaagacc cggacgtgat
    12001 ccaagtcgta caaagtggcg gtcagtatca agtagtgatt ggcagtaatg tagcagatgt
    12061 ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg gaggatggaa aagaagagtc
    12121 caaaaatatt ttgaatcgtc tgatcgatat tatctctagt atatttacac cttttctagg
    12181 ggcgatggca gccgccgggg tattgaaagg attcttatca ttggcaacgg tgatgggctg
    12241 gctgacagct gatagcggta cttatcaaat attgtttgct gcagcagatg gtgtctttac
    12301 cttcttacct gtcatgcttg cttttacagc agctaaaaag ttcaaagcca accagttttt
    12361 agcagtagca attgctatgg cacttgtgta tccagctatc acagcagttg ctggtgcggg
    12421 agaagctatc agtttctttg ggatcccagt gattctttca ccatctggtt atacttcttc
    12481 tgtcatacca atcattctag ccgtatgggt tcaaagtaaa ttagagccat tcgtaaaaaa
    12541 agtgattccg caatttctgc aaatgatttt agttccgctt gtcgttttag tagtgatggt
    12601 tccattaaca tttcttgctt taggaccaat cgggacggtt gccggtaatg ctttaggcgg
    12661 tctcttcaac tcgatttatg gatttagtcc aatcgtagca ggtctaatca tgggaagttt
    12721 atggcaagta ttcgtgatgt tcggcatgca ttggggattc gtaccgatca tgtttttgaa
    12781 tatcgaacaa tatgggtttg atgtattgat gccaatgctt cttccagcca tcttggcaca
    12841 aggtggggct gctttagctg tagcattgcg aacaaaagat acgaaacttc gagcattagg
    12901 gatttcttct acagtgactt cgttatttgg aatcactgaa ccaactgtgt atggtgtgac
    12961 attacctttg aagaaaccat tcattgccgc ttgtatttct ggaggtattg gtggagctat
    13021 tatcggattt tccggagtaa aagctttctc cagcagtttg gtcagcttat tgacgattcc
    13081 gactttcatt aatacggtgg atggtgtaga gtcaaatgtg acagtagcag taatagctac
    13141 aggaattgct tttgttcttg cattcgtagg tacattgatt ttaggatttg acgaacaaac
    13201 acaagacaat caattagaaa acaaacatgc gaatgcagga aaaccgatta cttcagcacg
    13261 gcatacctta aaaagtcctt taacgggtaa agtactgcca ttgagtgaag taccagacca
    13321 agttttttct tctggcgtga tgggaaaagg tatcgctatt gatccagaag taggagaact
    13381 agttgcacct gcagacggag agatcacgac tatttttcca actggacatg ctgtagggat
    13441 cacaacgact gacggagcgg agattttgat tcatatcggg atggatacag tagaattaaa
    13501 tgggaatggc tttgagatct tagtcaaaca aggggatctc gtcaaagcag gagatttatt
    13561 gattcgtttt gacatcgaag caattagagc agctggctat agcgtaatca ctccagttgt
    13621 cattacgaat actgatgcat ttgccgatat acttgaatta gaccaaaaag agattattgc
    13681 aaacgaagat gttctggcaa ttgtaaaata aggaggaaat caggatgcaa tcacgttttt
    13741 cagaaaaatt tttatggggt ggagcaactg ctgccaaatc aattagaagg ggcttatgat
    13801 cgagacggaa aaggactatc cgttgcagat gctatgcctg gaggtaagca gcgctttgcg
    13861 attatcggca gtgaagaatt tgactggacg attgatcaag aaaaatatat ttacccgaat
    13921 catcgaggaa tcgatcatta cgatcgtttc aaagaagatc ttgccttgtt tgcggaaatg
    13981 ggattcaaat gttatcgttt ttcgattgcc tggacacgaa tttttccaaa tggagacgaa
    14041 ggtactccta acgaagcggg attagaattt tatgatcagc tgattgatga gtgcttaaaa
    14101 tatgatattg aacctgtgat cacgatttct cattacgaaa tgcctcttca tttggcaaaa
    14161 gaatacggtg gctggaaaaa tcgaaaactg atcgacttct atgaacgatt tgctcagaca
    14221 gtattagaga gatacagcag taaagtcaaa tattggatga ctttcaatga aatcaattcg
    14281 gcctttcact ttccagcttt aagtcaagga ttagtaaaaa gcaatggagc aggagagtac
    14341 caaaatattt tccaagcatg gcataatcaa ttcgttgcaa gtagcaaagc cgtaaaaatc
    14401 ggccacgaat tgcgttcgga tatccaaatc ggctgcatga ttatctatgc aacaacttac
    14461 agcatcgatg ccaatccagt caatcaggca gcaacaatga tccaaaacca agaattcaat
    14521 tttttctgca cggatgtcca agtacgtgga gaataccctg catacacagc gcgtacccat
    14581 aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg aaaaagactt ccagttgcta
    14641 aaagaatatc cagtggacta catcggattt agttattata tgtctacggc aatcaatgag
    14701 actgatccac aagcagcaac ttcagaaggg aacttgctgg gaggagtaaa aaatccgttt
    14761 cttgaggcaa gtgaatgggg ctggcagatc gatccagaag ggctgagaat cgcattgaat
    14821 gaattataca atcgttacca gaaaccatta ttcattgttg aaaacggtct tggagccatt
    14881 gatcatatgg aagaggatgg cacgattatt gatgattatc ggatcgatta tttacgtcgt
    14941 catatcgaag cgatggctaa tgcagtagca gacggtgtag atttgatggg ctatacgcct
    15001 tggggatgta tcgatcttgt cagtgcatca acaggagaaa tgagtaaacg ttacggcttt
    15061 atctatgtgg atctagacga tgaaggaaat gggacactga atcgatcaaa gaaaaaatct
    15121 tttgattggt ataaagaagt aattgcctca aatggcgtaa acttgtaatt aaagcagctg
    15181 aatgaaaaag aacacacttc tcacatgtga tttgtgttct ttttttattt tttatgtgct
    15241 attcttttag taatagtaaa gcaaagggtg acgttatgaa aaggacacgt gtactatact
    15301 tggaagtcgc agataaaatt aaagaagata ttttctcagg aaaatatcca gtcggttcga
    15361 tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt cagcaaaatc actgtccgaa
    15421 aggcaatcga actgctggca tcagatgaat atgtcgcaaa gaaaagtggt cgtgggacga
    15481 cggttctaag caatcgtcct tacaatcgat tatccaaagc ggcaagcttt acgcaaatct
    15541 tggagaactc ccgatatgaa gtccggaaag aaacgctgga tttgcaattg gtccatataa
    15601 agaaggaaga tcctttgttt tcctttttcg gtgagcaagc tgtttgtttt aagcgcttat
    15661 attatttgaa caatcagcct tatatttatt ttgttcatta tcttccacaa gaaatgatgc
    15721 aattcagcag agaagaattt ggcaaagaat ctttgtatcg tttgctgacg aaaaaaggat
    15781 atactattga ctcattttct gatgatttct cggcagtatt tttaacagaa gaagaacaag
    15841 gaattctgaa aacagacgca cagatcggca tcaaacggat acggaaatcg tctacgcaaa
    15901 ttggggaaat gattgaatat tcggaggcgg tctaccatac ggagctgcat ccttaccata
    15961 ttgattatga aacataaata aaaaaagagg ttgtgaaaaa gctgccctgc atcaagtaat
    16021 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct tattaccgca
    16081 gatacacggc tgtttctgga ttaccattta ttctggataa gggggtgtgg cacgattttc
    16141 agaaggaatc tatgaaccaa tcactgtatt gattttagct tcgatggtaa ccccattcgg
    16201 cttgacgatc gcttatttct tagggaaaat cattcgtaaa aatatactca atcgtcaaga
    16261 aatcgataca ttgaagaccg cttttcctat ggggatctgt cagatcacag agggatgttt
    16321 ccctatcgta ttgaacgact tggcacgcaa tgtcatagca acgggagtcg gtgggacagt
    16381 aggtggcgga ttaagcatgt tttggggcgc agatagccgt attccggcat cgggaatgtt
    16441 tgcagtcgct acgatgacca gaccatgggc attcattggt gcattgttag ctggttcttt
    16501 tgtcacagga ataaccattt tattattgaa aaaaccagta gatccgaatg cagaaatcat
    16561 ccaagaagaa aaagaggaag aagacatatc ttgggatgat ttgacaatta gttaggagag
    16621 gcagaatgga ttacaagaaa aaagtacaat agatgtacct tgagacagga atggtatgaa
    16681 agaatcagag ttagaaagaa tcgaatatac agattttggt ttaggagcga tcgaaactgt
    16741 ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa aaatgagaac ctttcgttgt
    16801 cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac aaaaaaactt tcctgtacta
    16861 ttccaaaaag gaaagaaagt tattatacag taaaaaaaga aattattctt catgctagag
    16921 atcagtacac gttcgagccc aatatcaaac actagttcca ggcaggcgca acaggtgcgg
    16981 ttatcactga attttcttcc agcagtgacg attcaagtga tatttttaca aatccagcta
    17041 tttatcgtta acaaaaagat ttttaggaga agctgatgaa caaaaatgat caaattttga
    17101 tcgtttagtt cgtcggcttt tttatttaga gaaacatcat ttatcagtga aacataacac
    17161 gagaacaaaa tgattttttc gttaataaaa gaaacaagta tttgttgttt aaaataatac
    17221 acgttattgg catataggac ga
//
LOCUS       DAZAOG010000047        17224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000047 DAZAOG010000000
VERSION     DAZAOG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.047"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHDLD_001319"
     CDS             1..369
                     /locus_tag="ACHDLD_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN3363029.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHDLD_001320"
     CDS             549..1490
                     /locus_tag="ACHDLD_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN3363030.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHDLD_001321"
     CDS             1559..2290
                     /locus_tag="ACHDLD_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3363031.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHDLD_001322"
     CDS             2434..4614
                     /locus_tag="ACHDLD_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN3363032.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHDLD_001323"
     CDS             4634..5116
                     /locus_tag="ACHDLD_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN3363033.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHDLD_001324"
     CDS             5132..5893
                     /locus_tag="ACHDLD_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN3363034.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHDLD_001325"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHDLD_001325"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN3363035.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHDLD_001326"
     CDS             7665..8150
                     /locus_tag="ACHDLD_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN3363036.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHDLD_001327"
     CDS             8143..8826
                     /locus_tag="ACHDLD_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN3363037.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHDLD_001328"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHDLD_001328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN3363038.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHDLD_001329"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHDLD_001329"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN3363039.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHDLD_001330"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHDLD_001330"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN3363040.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHDLD_001331"
     CDS             13123..13455
                     /locus_tag="ACHDLD_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN3363041.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHDLD_001332"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHDLD_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN3363042.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHDLD_001333"
     CDS             14281..15654
                     /locus_tag="ACHDLD_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN3363043.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHDLD_001334"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHDLD_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN3363044.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHDLD_001335"
     CDS             16709..17149
                     /locus_tag="ACHDLD_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN3363045.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5874 a         3009 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DAZAOG010000048        16734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000048 DAZAOG010000000
VERSION     DAZAOG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.048"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACHDLD_001336"
     CDS             <1..828
                     /locus_tag="ACHDLD_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363046.1"
                     /translation="LNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITE
                     LISTNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVL
                     GLKWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQ
                     AKWQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDL
                     RHSNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1402..1971
                     /locus_tag="ACHDLD_001337"
     CDS             1402..1971
                     /locus_tag="ACHDLD_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN3363047.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1958..2818
                     /locus_tag="ACHDLD_001338"
     CDS             1958..2818
                     /locus_tag="ACHDLD_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3363048.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3008..3712
                     /gene="yycF"
                     /locus_tag="ACHDLD_001339"
     CDS             3008..3712
                     /gene="yycF"
                     /locus_tag="ACHDLD_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN3363049.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3717..5552
                     /gene="walK"
                     /locus_tag="ACHDLD_001340"
     CDS             3717..5552
                     /gene="walK"
                     /locus_tag="ACHDLD_001340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN3363050.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5549..6865
                     /gene="yycH"
                     /locus_tag="ACHDLD_001341"
     CDS             5549..6865
                     /gene="yycH"
                     /locus_tag="ACHDLD_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN3363051.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6866..7735
                     /locus_tag="ACHDLD_001342"
     CDS             6866..7735
                     /locus_tag="ACHDLD_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN3363052.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7790..8599
                     /locus_tag="ACHDLD_001343"
     CDS             7790..8599
                     /locus_tag="ACHDLD_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3363053.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8702..9331)
                     /locus_tag="ACHDLD_001344"
     CDS             complement(8702..9331)
                     /locus_tag="ACHDLD_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3363054.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9501..10793
                     /locus_tag="ACHDLD_001345"
     CDS             9501..10793
                     /locus_tag="ACHDLD_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3363055.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10869..11531)
                     /locus_tag="ACHDLD_001346"
     CDS             complement(10869..11531)
                     /locus_tag="ACHDLD_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN3363056.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11756..12040
                     /gene="groES"
                     /locus_tag="ACHDLD_001347"
     CDS             11756..12040
                     /gene="groES"
                     /locus_tag="ACHDLD_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN3363057.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12091..13716
                     /gene="groL"
                     /locus_tag="ACHDLD_001348"
     CDS             12091..13716
                     /gene="groL"
                     /locus_tag="ACHDLD_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN3363058.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13805..14944)
                     /locus_tag="ACHDLD_001349"
     CDS             complement(13805..14944)
                     /locus_tag="ACHDLD_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363059.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15055..15639)
                     /locus_tag="ACHDLD_001350"
     CDS             complement(15055..15639)
                     /locus_tag="ACHDLD_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN3363060.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15766..16197)
                     /locus_tag="ACHDLD_001351"
     CDS             complement(15766..16197)
                     /locus_tag="ACHDLD_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN3363061.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16215..16535)
                     /locus_tag="ACHDLD_001352"
     CDS             complement(16215..16535)
                     /locus_tag="ACHDLD_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3363062.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5494 a         2853 c         3385 g         5002 t
ORIGIN      
        1 ctaaataggt taaagcctta tcatttacaa gagttttatc aatatatgtt aaatgataac
       61 tgtacagcaa atacggttat tcattatcac gcatttatac gcaaagctct tcaagaggct
      121 gttattacag aattgataag tacaaatgta gcagataaag taagaaagcc aaaaaagaaa
      181 caatttgttt ctcaagttta taatcaacaa gagatcttga aattacttga cattttgtca
      241 gaagaaaaac tctatttggt agttctttta acagcatttt atggattgcg aagaagtgaa
      301 gttttaggat taaaatggtc tgctattgat tttttaaaca aaaaaatcat gataaaccat
      361 gtaatcatag aaaatccaga gaatatgtca cagttaataa agaaagatca aacaaagaac
      421 tcttccagct atcgtacatt acctttggtt gaaacgattg aacgagcatt agtaaatcaa
      481 gctaaatggc aacaagataa tcgtattctt ttaggagatg attatatttt agaagataat
      541 gagtacgtat ttactatgga agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt
      601 ttgagtaagt tgattaagaa aaatggattg aaaaaaattc gctttcatga cttgcgtcat
      661 tctaatgctt caataatgtt agatagtggc cagaatatga aatccattca agaatggctg
      721 ggacatgcat cgtattcgac aactgctaat ttatacacgc atttaaccgc aggagtaaaa
      781 gatcgtgcag cagaagcatt agaaaacgca tttgggttta aagaataaaa aaacaaaaaa
      841 caatatcttt ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa tttgttggca
      901 gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat ttataaagct ttataaaact
      961 tttttcacaa atagagcatt tggttaagag tcagctgctc taccaactga gctaacggcc
     1021 cgtacaaaaa gtacttaata atagtagcac taagaatttt aaaatgcaag actacattta
     1081 aagaaaatca gaaaaatctt tacttcctta taggaaatca ttcatagatt attcacattt
     1141 gctcgctatt atataagcat accaacaaac aaagttcttg caagagaaca aaccctaaca
     1201 cttttttcat tttttactcc tttcccgccg tctccccggc gggtctcctg ttcttaacga
     1261 taaaagacaa gaaggcttag ccttcttgtt ttttgttttc atggaatccc gctaatcaaa
     1321 tcacctagtt aaagcaacta gattgaatgt ttatcataag tctgatatca tgtttatgaa
     1381 atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag
     1441 gaactagaaa cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg
     1501 gaccaagtca gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat
     1561 ccattgctta cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca
     1621 gttaaaaaaa gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa
     1681 caagtattga ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga
     1741 attcgtgaag cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc
     1801 caattagcac aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat
     1861 attgctgtaa gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt
     1921 gttctagaaa ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat
     1981 tttagtagat tcaggtacgg atgttccaga agaattgata aaaaaatacg agatgtatat
     2041 gatcccattg aaaattattt ataaagatca ggtatataca gataaaattg acattacccc
     2101 tgaagagatc tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga
     2161 gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt
     2221 aacaatttcc agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca
     2281 agaagaattg gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc
     2341 tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa
     2401 tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct
     2461 tcaaaaaggt ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa
     2521 cccaatcatc tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg
     2581 taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt
     2641 cttcaactta gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag
     2701 acttaaaaga gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt
     2761 agtagtccac actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat
     2821 caaaaaaagg aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca
     2881 aaaaataata aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag
     2941 atgaaacaaa agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg
     3001 gctctttatg aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa
     3061 attcaattta gccaaagaag gctatgacgt ttatactgca tatgatgggg aagaagcctt
     3121 ggaaaaagtt gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat
     3181 ggacggctta gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt
     3241 aacagccaaa gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta
     3301 cgtgaccaag ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg
     3361 cggtgccacg gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga
     3421 tttgacgatc catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac
     3481 acatcgtgaa ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag
     3541 agaacattta ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga
     3601 tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt
     3661 agtgacacgt cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga
     3721 aaagaaaagt ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga
     3781 ttttattgat ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca
     3841 cgatcaatac atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca
     3901 gcccggaatt gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa
     3961 gatttattga aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag
     4021 ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt
     4081 atgttgatat caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac
     4141 gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc
     4201 tttacgtcaa aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt
     4261 tctttactgc ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc
     4321 gttcgattac acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag
     4381 actacagtcg gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct
     4441 tcaatcagtt agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc
     4501 gtttggatag cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta
     4561 aagtcattac gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa
     4621 tcggacaatc gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat
     4681 tagaagatcc aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga
     4741 tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag
     4801 tagctgtctt gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg
     4861 tctctaatgt ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag
     4921 cattgagcga aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc
     4981 tagatgaaac agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg
     5041 atacaggtaa tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg
     5101 ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg
     5161 aatttacgca aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg
     5221 ataatatcat gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct
     5281 tattagaaac gcataataac gtgattttaa gtatcaccga tcaaggattg gggattccta
     5341 aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag
     5401 cacaaggtgg gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag
     5461 gcgctatttg ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat
     5521 atgaacctta tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg
     5581 actcattttg atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc
     5641 aggaaaaagt tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa
     5701 tcgaaaaact tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat
     5761 ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag
     5821 atttggaaag ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa
     5881 tctgaactct ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga
     5941 agcatttgat ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt
     6001 tactagaata caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca
     6061 tctcgtatat gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc
     6121 aaaagacaca aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc
     6181 tacgaaacat tcgatcaaac tgaagaagta tagttatatc ctgtcacagc aaccctatac
     6241 actttttaga aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa
     6301 tgaattgatt ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt
     6361 tgattttcgt ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt
     6421 cccctatatt agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga
     6481 caatacgatc gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag
     6541 aggacagatt ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca
     6601 aaccagtttg aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag
     6661 tacgaatgaa atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc
     6721 agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt
     6781 acctgaatgg tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa
     6841 attacctgga ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt
     6901 cgctttttta gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga
     6961 aaacaatgtc attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga
     7021 tatcacttac acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga
     7081 gcagaccaat cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct
     7141 ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc
     7201 tgaaaagaac gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa
     7261 caaagacaat attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga
     7321 agaagaacca ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga
     7381 tacagcaaaa atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata
     7441 catgcagacg catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga
     7501 agaggaagca atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt
     7561 atggcgtcag ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc
     7621 tgtttggcat gtagcgatcg aaacaagcga taaagtggtc caaattgaga gcgtcaacgc
     7681 tttcagcaat acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag
     7741 cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga
     7801 aaaaccagct tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat
     7861 cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc
     7921 cttgatggct caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga
     7981 acatcgagat catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt
     8041 tgccaatcag cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca
     8101 aaaacatcta tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt
     8161 cggcgtctcc catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc
     8221 attcgtcatg ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa
     8281 tgccgatggt tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta
     8341 tccgtggaat ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg
     8401 tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct
     8461 tagcaaggaa aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga
     8521 aaaagatttt ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac
     8581 tgagctattt tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat
     8641 caatcagggt tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt
     8701 attatcgact gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta
     8761 ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc gctagcagcg tttctttgta
     8821 aataatcatc acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag
     8881 ctggactgat ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt
     8941 acatattgaa agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag
     9001 tcgtcttttc tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat
     9061 cggttccttc aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc
     9121 ctttgggagc gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca
     9181 actcaaataa atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca
     9241 cgattgccgt cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat
     9301 ggccagtagc tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat
     9361 tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt
     9421 cctttcatgg ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata
     9481 gaagttgaag gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa
     9541 catctgttgt gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc
     9601 caataattat tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg
     9661 ctcagtggat agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat
     9721 ttgatgtgat agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag
     9781 acatcgatcg cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc
     9841 cactaaatac tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta
     9901 caactgggct catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga
     9961 agcaaagaga gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca
    10021 ttgttatagt cacttttacg cttatcggtt cagcggtttt tcaacatttg ttcagaatga
    10081 ctgttgttca aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt
    10141 tgtttggtgt gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg
    10201 gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcaaatg
    10261 cagaaatctt agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc
    10321 agcaatttct cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat
    10381 tttttatgac ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt
    10441 taccagcctt tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc
    10501 cgatgatggc tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg
    10561 ctttatattt tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc
    10621 cgatcctctc gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt
    10681 tgtcaattag ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca
    10741 aaaaggaaag acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa
    10801 aaatactcct gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat
    10861 agtcatgttt atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc
    10921 attcctacgt gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg
    10981 aagaagaaac ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac
    11041 agcaaagagc tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt
    11101 atactgcggc ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata
    11161 aaagcgggtg cttccatgat catctggatg atattttgtg tattctcgga aggggttgct
    11221 ttgccaaaca atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag
    11281 attccaacca atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg
    11341 atcgctaagg gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata
    11401 tacatgactg cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct
    11461 aggatcaagg gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt
    11521 tttaaagaca tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc
    11581 gattgatttc aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct
    11641 ttgatttaaa agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca
    11701 ctcgaaggtt aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt
    11761 aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg
    11821 aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt
    11881 cggtgaaggc cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga
    11941 ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct
    12001 gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata
    12061 atcaaaaata atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac
    12121 gcacgcgcag ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta
    12181 ggacctaaag gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat
    12241 gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca
    12301 aaacttgtat cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca
    12361 gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct
    12421 aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg
    12481 cataatatct caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct
    12541 tcgggttctg ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac
    12601 ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt
    12661 atgcagtttg atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa
    12721 gctgttctag aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat
    12781 attctgccat tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac
    12841 gatgtagatg gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac
    12901 gtagtagctg taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc
    12961 gctattttga caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgca
    13021 acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc
    13081 gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa
    13141 attgctgaaa caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta
    13201 gcaggtggtg tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta
    13261 aaattacgca ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc
    13321 tcaggtggtg gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa
    13381 ggagacgtgg caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa
    13441 atcgctgaaa acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa
    13501 ctaggcactg gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc
    13561 gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg
    13621 ttattaacga cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct
    13681 gcaatggatc catcaatgat gggcggtatg atgtagtaat taagaaagaa ccctatttta
    13741 acagggttct ttcttctttt tttgttttat tttaagcaaa aggggcaaag aaggggcaaa
    13801 aatatcaaag gctatttagt ttatcaatta cttgttgttt ggctcttttt gttacatggt
    13861 tataaataga cagagttgtg tttgcatcag aatgccctac acgctccata atggctttaa
    13921 gaggtacgcc taattcagat aatagtgaaa cgtggctatg tctgaatata tgtgaggata
    13981 gacttttttc taattccaac tcttcctcta ctttatggag tatagcgtta aatgagtgca
    14041 gcgcaagcgg agtgccactt gtagatataa atatatattg atcgggatct gtgggtctac
    14101 ctgcaagaat attgtcagct atcacacttt caatcaattc ttttgcacga ttgggtaatt
    14161 gcacttcacg ttgcgaataa gtattttttg gagttgtttt tatagcatta tccattttca
    14221 cagatgtgta atctaaggtc ccattaatgg aaattttccc atcctcatag tccttcattt
    14281 gcaaagctag caattcccca tatctcaaac cagttaaata tagaaactca gctattatgc
    14341 cgtgtagttt tctgcgagga ttggagtata gctgtttcag tatttgatca atttcttctt
    14401 tctccagata ttttttattc atagaaagcc ttcttttttc ttcttccact tttttaggat
    14461 ggattttaac tgctagcgca gggtttcttt gaatgtattt tctatcgatt gcatagttta
    14521 gcataacaga tagagttgtt tttgtttgtt ttgtgtagtt cagtgagagg tcaccaaacg
    14581 tatacatatc ttcaattatc ttattaatga gtgtctcatc aatgtttcta acgatcgtat
    14641 catcgcttat gtgcttagaa acatgtttca tcatcattgg aaccttcaaa tagctagtac
    14701 gtttaacatg ctgcttataa tattcatacc attctttata cagctcacca aaagtgatat
    14761 ctgatttatt gtaatcttcg agtgcttctt taattttttt atctagaatt ttctgagctt
    14821 ttttccacgc ttgtggtgaa ttacttgtaa gtgttgtaga tttttttcgt gttttttctg
    14881 tataaggatc tacatatctt tcaataaact tgaattggcc atctttggtc tgttcaaccc
    14941 acacttttaa catctcctat catttgctat aataggcata acaaatagac ctatataggt
    15001 ttgttttcta aaagcacgct cttactctgg acggtggggc gtgtttttta tttattatac
    15061 agttgcttta aaaatagctg ctttttcaaa tgatactaaa ggagagaagt ggatttctat
    15121 ttctcccttt tggtttaacg tgaaatgagc agttacatcc atttgttttc ctggagcaac
    15181 agatcccatt gtattttcgt tggcatatgt ctctgatttt ttgtcatctg gtccatatac
    15241 ttccacatct gtacctacag ggatatctga atcgccatca ttttttacgg tataagtaat
    15301 ttttactact tgtgcaggtt ggttttcttc aaattgattt ctttcatcag ttagttctac
    15361 actatttagt gtatattcag catcaccaac ttttacagtg tcaccaatct tatagaaggt
    15421 atcgcttttt tcttctttag aagatgaagc agaggttgaa gatttagtta ctttttcgcc
    15481 accattatca cttgctttat tactatctga tccaccattt aaagcagaac caataattat
    15541 aattaaaatt actgctaata cccaaaacca tacttttttg taaaaaggtt tactaacttt
    15601 atacattttt ccgtcttgac ccataacttt ttttgccatt taaatattcc tcacttcttg
    15661 ttataatata tttgcgatct cagaaatgag gtatgagtcc gtgtgccagc acgggctttt
    15721 ttaatgtttt gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct
    15781 tgcgtaagaa tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag
    15841 cttattctga atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt
    15901 cagttcgttc tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt
    15961 ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa actgaatatt caaaatgaga
    16021 agcggtgaaa tgcgcaagtt catgagataa atgaaaggct tctgcagttt cgccatatag
    16081 attttcattc aaaaaaatga ttctaggctt cggataatag aagccaggct ctttcatttc
    16141 catgtaaact actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca
    16201 acccatcact ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc
    16261 attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtt ttctgataaa
    16321 tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac agagactcca
    16381 aagtaatcag ctaaccgagc taactcaata gaattaggag tttgtctttt ccaacttcct
    16441 aaatatccat tcgaataccc gaaattcatt tcgagctgtc taatggataa tcctttttct
    16501 tttgctaact cctttattat ttcgtaagta ttcattgata aatcaacctt tctgaatgct
    16561 tacaaaaaaa gtttagataa ataatgtaaa aatagttgac ttatatacat tattaatcta
    16621 tactatgttt tgtaaacaag tttaacaact aaaaagacaa caaaaaatac tattgattaa
    16681 taaatgccaa ccgccaagaa agctttttaa atcaaagttt atatgtctta ttta
//
LOCUS       DAZAOG010000049        16680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000049 DAZAOG010000000
VERSION     DAZAOG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.049"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHDLD_001353"
     CDS             complement(29..727)
                     /locus_tag="ACHDLD_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN3363063.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHDLD_001354"
     CDS             complement(720..2072)
                     /locus_tag="ACHDLD_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN3363064.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHDLD_001355"
     CDS             complement(2295..3695)
                     /locus_tag="ACHDLD_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN3363065.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHDLD_001356"
     CDS             3856..4593
                     /locus_tag="ACHDLD_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN3363066.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHDLD_001357"
     CDS             4718..5197
                     /locus_tag="ACHDLD_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN3363067.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHDLD_001358"
     CDS             5194..5961
                     /locus_tag="ACHDLD_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN3363068.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHDLD_001359"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHDLD_001359"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3363069.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHDLD_001360"
     CDS             complement(6864..7466)
                     /locus_tag="ACHDLD_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN3363070.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHDLD_001361"
     CDS             7572..7802
                     /locus_tag="ACHDLD_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363071.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHDLD_001362"
     CDS             7793..8041
                     /locus_tag="ACHDLD_001362"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363072.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHDLD_001363"
     CDS             8147..8731
                     /locus_tag="ACHDLD_001363"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN3363073.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHDLD_001364"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHDLD_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN3363074.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHDLD_001365"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHDLD_001365"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN3363075.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHDLD_001366"
     CDS             10632..10811
                     /locus_tag="ACHDLD_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363076.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHDLD_001367"
     CDS             10833..11858
                     /locus_tag="ACHDLD_001367"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN3363077.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHDLD_001368"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHDLD_001368"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN3363078.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHDLD_001369"
     CDS             13430..14377
                     /locus_tag="ACHDLD_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN3363079.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHDLD_001370"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHDLD_001370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN3363080.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHDLD_001371"
     CDS             complement(15279..15521)
                     /locus_tag="ACHDLD_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363081.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHDLD_001372"
     tRNA            15642..15714
                     /locus_tag="ACHDLD_001372"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACHDLD_001373"
     CDS             complement(15833..16471)
                     /locus_tag="ACHDLD_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN3363082.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..>16680)
                     /locus_tag="ACHDLD_001374"
     CDS             complement(16594..>16680)
                     /locus_tag="ACHDLD_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3363083.1"
                     /translation="RSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5683 a         2967 c         3345 g         4685 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
//
LOCUS       DAZAOG010000050        16432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000050 DAZAOG010000000
VERSION     DAZAOG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.050"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..2684
                     /locus_tag="ACHDLD_001375"
     CDS             213..2684
                     /locus_tag="ACHDLD_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3363084.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2787..4523)
                     /locus_tag="ACHDLD_001376"
     CDS             complement(2787..4523)
                     /locus_tag="ACHDLD_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3363085.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4520..5224)
                     /locus_tag="ACHDLD_001377"
     CDS             complement(4520..5224)
                     /locus_tag="ACHDLD_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3363086.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5355..5858
                     /locus_tag="ACHDLD_001378"
     CDS             5355..5858
                     /locus_tag="ACHDLD_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN3363087.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5818..6156
                     /locus_tag="ACHDLD_001379"
     CDS             5818..6156
                     /locus_tag="ACHDLD_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN3363088.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6177..7595
                     /gene="ffh"
                     /locus_tag="ACHDLD_001380"
     CDS             6177..7595
                     /gene="ffh"
                     /locus_tag="ACHDLD_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN3363089.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7707..8285
                     /locus_tag="ACHDLD_001381"
     CDS             7707..8285
                     /locus_tag="ACHDLD_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN3363090.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8289..8810
                     /locus_tag="ACHDLD_001382"
     CDS             8289..8810
                     /locus_tag="ACHDLD_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN3363091.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8889..9443)
                     /locus_tag="ACHDLD_001383"
     CDS             complement(8889..9443)
                     /locus_tag="ACHDLD_001383"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN3363092.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9696..9971
                     /gene="rpsP"
                     /locus_tag="ACHDLD_001384"
     CDS             9696..9971
                     /gene="rpsP"
                     /locus_tag="ACHDLD_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN3363093.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9983..10234
                     /locus_tag="ACHDLD_001385"
     CDS             9983..10234
                     /locus_tag="ACHDLD_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN3363094.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10359..11150
                     /locus_tag="ACHDLD_001386"
     CDS             10359..11150
                     /locus_tag="ACHDLD_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363095.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11320..11841
                     /gene="rimM"
                     /locus_tag="ACHDLD_001387"
     CDS             11320..11841
                     /gene="rimM"
                     /locus_tag="ACHDLD_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN3363096.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11831..12592
                     /gene="trmD"
                     /locus_tag="ACHDLD_001388"
     CDS             11831..12592
                     /gene="trmD"
                     /locus_tag="ACHDLD_001388"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN3363097.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12728..13075
                     /gene="rplS"
                     /locus_tag="ACHDLD_001389"
     CDS             12728..13075
                     /gene="rplS"
                     /locus_tag="ACHDLD_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN3363098.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13207..14343)
                     /locus_tag="ACHDLD_001390"
     CDS             complement(13207..14343)
                     /locus_tag="ACHDLD_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363099.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14459..15373)
                     /locus_tag="ACHDLD_001391"
     CDS             complement(14459..15373)
                     /locus_tag="ACHDLD_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN3363100.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15464..15892)
                     /locus_tag="ACHDLD_001392"
     CDS             complement(15464..15892)
                     /locus_tag="ACHDLD_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN3363101.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15897..16271)
                     /locus_tag="ACHDLD_001393"
     CDS             complement(15897..16271)
                     /locus_tag="ACHDLD_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3363102.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5307 a         2961 c         3227 g         4937 t
ORIGIN      
        1 ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa
       61 cacaaaatat tatagaaccc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa
      121 gcttaatgtg aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat
      181 atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta
      241 aaaaaaagaa agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata
      301 aagaaggatt aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt
      361 cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt
      421 tcaatttgat ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact
      481 tgactttttt accagttatc atagccaata caggaatcgc catttttcag gaaattcgat
      541 ccaaaaagat cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag
      601 acgggataga acaaacaata gatatagaag accttgtaat agatgacatt gttattttag
      661 agactggata tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg
      721 aggctttatt gacaggcgaa gcagatgaaa taaccaaaga aataggtgat ccattaatgt
      781 ccggcagttt cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt
      841 cgtatatttc ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga
      901 tgattgcttc tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta
      961 tcctgctttt ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg
     1021 caatggaagc agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg
     1081 cattagcatt aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa
     1141 gcattgagac attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag
     1201 aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa acaagaaata atcagtcagt
     1261 cagatcaatt ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa
     1321 caatggaagc tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta
     1381 ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt
     1441 taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt
     1501 ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag
     1561 aaccagtatt gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca
     1621 gaaaagaagc aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga
     1681 tttctggaga taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg
     1741 ctgatcagta cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa
     1801 aatatacagt ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa
     1861 agcgtaacaa tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag
     1921 aagcagactg cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag
     1981 tggtcttact tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg
     2041 ttgtgaacaa tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt
     2101 tgctctcact gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca
     2161 cattgattag tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca
     2221 aaaagcgaat cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac
     2281 taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata
     2341 aaacagatgt ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct
     2401 ataaaatctg ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg
     2461 gtaacttttt ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg
     2521 tatcactttt gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga
     2581 ccacattggt agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc
     2641 gcgtagctaa aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa
     2701 aaagagtaaa aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt
     2761 taaaacgcac gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa
     2821 cctgttccaa gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt
     2881 acaatcgcta accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg
     2941 taaaaacgct caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg
     3001 acagaaaacg atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt
     3061 gaatattgga cagcattttc aattagattt ttgataattg gataaaggat atctgtttta
     3121 gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt
     3181 ggctgataat tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg
     3241 taagaattct cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg
     3301 atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca
     3361 ccatccagta aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga
     3421 gagacgttgc caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca
     3481 tacgccaccc ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag
     3541 tctaatgtct gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga
     3601 gtcacttgat gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt
     3661 tcaggttgat agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa
     3721 cgagaatcag catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat
     3781 aattgctctt ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca
     3841 ttaatcgtct gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga
     3901 ttaggatttt gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt
     3961 tgatagatca gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa
     4021 aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg ttctgcgata
     4081 cgtaaaatgg cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc
     4141 tcattcaaag tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct
     4201 ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag
     4261 atctttccag tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc
     4321 aaagcagttt tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt
     4381 tgtgcttttt ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg
     4441 gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt
     4501 tcataaattt ttttccattt catcgtttgg gttcctgaaa cttatagcca aatcctctaa
     4561 tagtcaccaa gtactttggg tgttttggat ctcgctcgat tttttctcgc aaatgactaa
     4621 cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca
     4681 atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt
     4741 ctttcggtgt tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag
     4801 cagtgatttg tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag
     4861 gttcaattcg tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt
     4921 tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt
     4981 tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct
     5041 ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta
     5101 atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt
     5161 ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt
     5221 tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac
     5281 aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa
     5341 aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg
     5401 atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca
     5461 tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg
     5521 gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag
     5581 atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac
     5641 acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg
     5701 acggaagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc
     5761 agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg
     5821 gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca
     5881 gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc
     5941 gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag
     6001 atcttggagg attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag
     6061 gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa aaaactactt
     6121 gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg
     6181 catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa
     6241 aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag
     6301 ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag
     6361 gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag
     6421 aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa
     6481 ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag
     6541 ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc
     6601 gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg
     6661 atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag
     6721 aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat
     6781 tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg
     6841 tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag
     6901 gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat
     6961 cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg
     7021 acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc
     7081 tgacattgat tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac
     7141 agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga
     7201 tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg
     7261 gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt
     7321 ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa
     7381 ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg
     7441 aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc
     7501 aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca
     7561 agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag
     7621 actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa
     7681 ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta
     7741 tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc
     7801 gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa
     7861 gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag
     7921 aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg
     7981 aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt
     8041 cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag
     8101 aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag
     8161 gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca
     8221 atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg
     8281 aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa
     8341 cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg
     8401 aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa
     8461 aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc
     8521 acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa
     8581 aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca
     8641 aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat
     8701 taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag
     8761 acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa
     8821 aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt
     8881 tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg
     8941 aagaaactta atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt
     9001 ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt
     9061 agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct
     9121 ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc
     9181 aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc
     9241 ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc
     9301 tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc
     9361 aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata
     9421 aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt
     9481 acaaaagata agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat
     9541 agattactta tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga
     9601 atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca
     9661 tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac
     9721 gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc
     9781 gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag
     9841 tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca
     9901 ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct
     9961 caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg
    10021 tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta
    10081 cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg
    10141 tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt
    10201 gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg
    10261 agtgcagctt ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa
    10321 gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta
    10381 ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac
    10441 atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg
    10501 cttccaccac tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc
    10561 agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct
    10621 atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag
    10681 atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc
    10741 tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga
    10801 tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg
    10861 cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga
    10921 ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag
    10981 aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga
    11041 cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc
    11101 aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg
    11161 ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc
    11221 atttttgcat aattttggct taatacctta aaagaaaact agaattgcct cttcacacat
    11281 gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta
    11341 ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat
    11401 ttcccagaag aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca
    11461 cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt
    11521 gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca
    11581 aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg
    11641 catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc
    11701 aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct
    11761 tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga
    11821 ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat
    11881 gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa
    11941 cttccgcgat tattcagata acaaacatca gacagtagat gattatccat acggcggtgg
    12001 agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc
    12061 tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca
    12121 gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga
    12181 gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt
    12241 ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc
    12301 agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga
    12361 acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac
    12421 aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta
    12481 tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt
    12541 agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct
    12601 aaaatcttct ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac
    12661 ttaactatta cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa
    12721 gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc
    12781 agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga
    12841 acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac
    12901 ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac
    12961 accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta
    13021 ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatc
    13081 gttatcagac tgagaaagaa cccttggtat tcaagggttc tttttttgtt ctctgttttt
    13141 ttatattaac gatttctatt tgggacattt ttgattaata aaaaggggca aaaaaggggc
    13201 aacaagttac agtttttcta tatcttcttt caatttttct tttactccct ttgtcacatg
    13261 caaataaatc ttttcagtga tatcactatt ttcgtgccca acgcgatcct gaatagcata
    13321 cagaggagtt cctagttccg ctaattttga aatatgggta tgtctaaaaa tatgtgaact
    13381 aagtttttta tcaatcttca tgtcagcttt atgatttctc aaataggtgt taattgctgt
    13441 tagttggaaa ggagttcctt tcgttgtttg aaatagaaat tgaccattcg gatttaattc
    13501 tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa tcaatttctc gcattccagc
    13561 agcagttttt gtagaatcag attttttcat atcagctatt gaacgttctc tatacatcat
    13621 cgtcccattt ataactactg atgcattatt attggtaatg tgtacatcgt ctttacttaa
    13681 tgcgattgct tctccaggcc tcattccagt caaatatagc cactggaaaa ggagagaata
    13741 tcttttattg tgtgaagttg tgaaatttac tagtctttta tattcatcat cttctaaaaa
    13801 cttatcttta atttttatcg tttttgactc tcgtttataa tcaataacta cttcgttgat
    13861 aggatttttt tctacatagc cttttttcat agcgtatgaa aaaagaagat tcaatttgga
    13921 tttaattaca ctgacgtact tatttgacaa gtcatcttta tatatcatat cttcaaaaat
    13981 attatttaaa tctactgtat taataccaga aacgatgtaa gtctcaggta tctttttctt
    14041 tatagtgttt aagatattat tcgtaggata atatgtagac tccttaactt gtcttttgta
    14101 aataactaac cattcttcga caagctcatg aaatgttaga tcaggcttct gaagtgtttt
    14161 ttcgtttaat tttatatcaa tttttttatt taactctagc aatgccactt tctgagtttc
    14221 tcgagattta tttttataag taatgctcac tttttttctt tttctagttt ttggatcaat
    14281 atatcgctca ttatatttat atactttttt tccgttttta tcagttttgg tttcaatcca
    14341 cattgttttc atctcctatt tgttgctata ataggaatgg ataagtaagc caattatagc
    14401 aggtttattt ttcatcacgt ccacaaactt tggcgaggga gggggcgtgt ttttttgttt
    14461 agtagctacc tttccatgaa gtatctgcga agaacacttc aaatgttgtt ccatcatttt
    14521 tcacttcaaa atatgctgtt ccttgtgcgg atttacccgg ttggatggtt tccgaaaagt
    14581 aatctctact agctaaatca gatttaatat ctgatgaatc ataaaattct aaataacttg
    14641 aacttgcatc aaaaggtgta gtccctgtgt tttctacaag aaaatctacc tttgcgtaaa
    14701 ataggccttc tggtttatag aaatcatcac cgtaatcttt tgatacagac tggacagtta
    14761 cgttaataga gttaccattt tcagaagtaa aaggaactgc ttctcctata ctatgtgcaa
    14821 ctgatgccgt ctggggttct gaagacgaag ttgtagttgt agttgtagta gagcttgaat
    14881 ctttggattt tccatcttta tctaagaaag ttcgttgatt ataatataca acctgttcag
    14941 tttttggact aacccaaatt aacattgttt catcatcaga atcagttgaa taagtatatt
    15001 ggagcatctc taattttttt atatcttttg cactatttcc atgttcaatt aattcagagt
    15061 gctctttata ataatctcct tgtgcatcct ttgaaataga tacaattaat tcatcccgtg
    15121 tgatagcatt gtctaattct ttgtaaacaa aatcatttgt ccatttacgt aacggtgaac
    15181 caagtgtctg ataaacttga gttaaatcat caccaacttt tattttatga acgtctgatg
    15241 aagttagttt ttttgtttca tattcagatg ttgatgaaac agtattttct ttagtgctag
    15301 cagaattagg actagtggag catccgctta ataccaagcc taaaaaaagc aacccaaaaa
    15361 caattttttt catttttaat tcctcacttc tatgatatga tttttttgta ggatcttaga
    15421 aataaggttt ttagtccgtg ttgcagcacg gacttttttt acattatata agaaagcgat
    15481 aagcgctttc tggaagtccg taaagattct ttaattcctc aattcgttta ggatattgat
    15541 cattgtcttc tttgtataga gaaacaatga gattagcagc aaagcaatta gcttcgcttt
    15601 cagatttgct tctagatgtt cttgttgata catagtaact ggataagcca cgatgaaaaa
    15661 tagcgtgacc taattcgtga gcgcaaatgt agaatctttc ctcagagtct ctcagttcat
    15721 catttaagaa gattattgca cgacctctaa tttcttgaaa ctgtcctttg ggattctcga
    15781 taaaaggaac gtattgaatt ttaatgccca tcttttcaca aatataaaaa ggattagcgg
    15841 actggtattt ccgcttcaac tcctcgacta aattaatcgt atccatctcc ataagctcac
    15901 atctttttgc ctttttcttt gtcttctttc acaatatccc agaaggttcc aataaggata
    15961 tcttttacgc gttggacctg ttcaggcgtc agtgtttctc cgccataaga catattgaca
    16021 tttgaatcta gtaatttatc aagttcaact acttcatctt ttgttgccca gtcaggaact
    16081 tggtttctac cgagtaaata atcagtagtt acattaaaat aatctgctac aagttgtagt
    16141 cttttagtac taggagttct atttttccat tgataaatcg tgtttttagg tatatttaat
    16201 tcttcttcca attgagttat acttatgcct cttttatgag ctaattcctt tattctatct
    16261 agtaaattca ttttcgcacc tcacaagcta cgaaaacact aataaaaaaa ttagttaaag
    16321 gtgttgacaa ctaataaaag tgttagtata ctgttttcgt aagctaaaat attagctaaa
    16381 aagacttgat aactaataaa cacttcacgg tcggcaaact ctgaaatgta aa
//
LOCUS       DAZAOG010000051        16424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000051 DAZAOG010000000
VERSION     DAZAOG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.051"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHDLD_001394"
     CDS             complement(152..550)
                     /locus_tag="ACHDLD_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN3363103.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHDLD_001395"
     CDS             complement(547..1320)
                     /locus_tag="ACHDLD_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN3363104.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHDLD_001396"
     CDS             complement(1317..2000)
                     /locus_tag="ACHDLD_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN3363105.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHDLD_001397"
     CDS             complement(2167..3054)
                     /locus_tag="ACHDLD_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3363106.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHDLD_001398"
     CDS             complement(3051..3920)
                     /locus_tag="ACHDLD_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3363107.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHDLD_001399"
     CDS             complement(4057..5343)
                     /locus_tag="ACHDLD_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3363108.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHDLD_001400"
     CDS             5616..6194
                     /locus_tag="ACHDLD_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3363109.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHDLD_001401"
     CDS             6194..7123
                     /locus_tag="ACHDLD_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363110.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHDLD_001402"
     CDS             7120..8856
                     /locus_tag="ACHDLD_001402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3363111.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHDLD_001403"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHDLD_001403"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN3363112.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHDLD_001404"
     CDS             complement(9752..10261)
                     /locus_tag="ACHDLD_001404"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN3363113.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHDLD_001405"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHDLD_001405"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN3363114.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHDLD_001406"
     CDS             complement(11562..12653)
                     /locus_tag="ACHDLD_001406"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN3363115.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHDLD_001407"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHDLD_001407"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN3363116.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHDLD_001408"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHDLD_001408"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN3363117.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHDLD_001409"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHDLD_001409"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN3363118.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4746 a         3628 c         2918 g         5132 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatc
//
LOCUS       DAZAOG010000052        16242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000052 DAZAOG010000000
VERSION     DAZAOG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.052"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            303..2138
                     /locus_tag="ACHDLD_001410"
     CDS             303..2138
                     /locus_tag="ACHDLD_001410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3363119.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2198..2914)
                     /locus_tag="ACHDLD_001411"
     CDS             complement(2198..2914)
                     /locus_tag="ACHDLD_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN3363120.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3141..3683)
                     /locus_tag="ACHDLD_001412"
     CDS             complement(3141..3683)
                     /locus_tag="ACHDLD_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN3363121.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3688..3915)
                     /locus_tag="ACHDLD_001413"
     CDS             complement(3688..3915)
                     /locus_tag="ACHDLD_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN3363122.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3994..5874)
                     /locus_tag="ACHDLD_001414"
     CDS             complement(3994..5874)
                     /locus_tag="ACHDLD_001414"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3363123.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6063..6677)
                     /locus_tag="ACHDLD_001415"
     CDS             complement(6063..6677)
                     /locus_tag="ACHDLD_001415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363124.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6819..7379
                     /locus_tag="ACHDLD_001416"
     CDS             6819..7379
                     /locus_tag="ACHDLD_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3363125.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7684..7998)
                     /locus_tag="ACHDLD_001417"
                     /pseudo
     CDS             complement(7684..7998)
                     /locus_tag="ACHDLD_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(8252..9887)
                     /locus_tag="ACHDLD_001418"
                     /pseudo
     CDS             complement(8252..9887)
                     /locus_tag="ACHDLD_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10442..11005)
                     /locus_tag="ACHDLD_001419"
     CDS             complement(10442..11005)
                     /locus_tag="ACHDLD_001419"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3363126.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(11020..12063)
                     /locus_tag="ACHDLD_001420"
     CDS             complement(11020..12063)
                     /locus_tag="ACHDLD_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363127.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(12196..12600)
                     /locus_tag="ACHDLD_001421"
     CDS             complement(12196..12600)
                     /locus_tag="ACHDLD_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3363128.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(12833..13468)
                     /locus_tag="ACHDLD_001422"
     CDS             complement(12833..13468)
                     /locus_tag="ACHDLD_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN3363129.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(13734..14786)
                     /locus_tag="ACHDLD_001423"
     CDS             complement(13734..14786)
                     /locus_tag="ACHDLD_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3363130.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(14799..15737)
                     /locus_tag="ACHDLD_001424"
     CDS             complement(14799..15737)
                     /locus_tag="ACHDLD_001424"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3363131.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         5087 a         3234 c         2603 g         5318 t
ORIGIN      
        1 tatgtaattt ccctttagtt tcttatcgcg tatctacatt tatgaaacgt gaatttccaa
       61 actcagggaa ggcatggctg ataaatatcg ggcagttttt tatttgatat ttattagcag
      121 tgtccctcct agttatcttt ttattttata tacaaaaaat ccgtttcatt catcgtttca
      181 tcgaatactt cgcttttgat tcgattgaac aattaactca gcgttggaaa aaaagaattc
      241 attggctttt tgttcataaa agttattttt tagttgcagc atttttttac tgctttacct
      301 tcgtgcagat tttcgtttta gaaccaaaag aaggttggaa agaaactatc caggttgcct
      361 taaaaatatt tactcagcga caagcaccca tgatcctgac aatcatcatt attatgggat
      421 ttttcttctt actattgttg atttccaatc gtttttggta cgccttgact gctaccttga
      481 tcattaactt actgctcact atttcgacag taatcaaaat ggaaatgcgg gaagagcctg
      541 tctttccttc agatttaaaa atgctgaccg gattgtcaga actattatcg atggttagcc
      601 cagtcttatt aatcgtgggc ggattgatcc ttctcctatt attgattact agtattattg
      661 tacaacgtag actacaaaaa cagtattcct tgaagattca ttggaaacgt cgatttatca
      721 gtatcgctgt attattagga tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc
      781 cttctttctt gctgtttaat ttatttaaag taaacaagac tttctttaac cagcaaggcg
      841 ccgtaaaaga taacggacca gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg
      901 aagaacctgc tggctattcg aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa
      961 aggcagatga aatcaaccaa acaagaagtg actggttaaa caatcagacg atcgttctca
     1021 atttaagtga aagcttcagt gatcctgcac gtgtccctaa tctgacagta cctacagacc
     1081 ctattcctta catcaaaagc attataacag aaggaacagg aggattgatg ttatccgtcg
     1141 gttatggtgg cggaacagct aacatggaat gggaaggact gacaggcttg agtatcagta
     1201 acctgtctgc ttcgttagtt acaccttata cccagctagt ggagcgtcag aaaataagtc
     1261 caaatattac cgatctattc gatgaaaaaa tagccatcca tccttttaca gctgctcttt
     1321 atcgcagaaa acaagtgttt gataaatttg gttttgacag gttctattat ttagacagtc
     1381 cggataaatt aacttatacg gataaaatag aacaaagccc acgcatatcg gatgattcag
     1441 catacaaaga aaccttgaaa gctttacaaa gcaatacgga aacgtcacaa ttcatccaat
     1501 tatccactat gcaaaaccat atgccttatg atgattacta tagtgaattg aactacactg
     1561 ctgaaggaac tgctgtcatt cctaatagaa gaaaagaatt acaaacttat atgcaaggcc
     1621 ttcactatac cgatacagca gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac
     1681 cgattacctt tgttttttat ggtgaccatt tacccgcaat ctatagtggg aatgattcga
     1741 aaaaatatgg attagaacag caccagacag attatttcat ttataacaac aagtatagtc
     1801 gtgaacaagc agctacgtta aataaagaag ttgttgctcc taataacttt ggtgctatgg
     1861 ctcttgagca tgcgaatatc aaagtaactc cttactatgc gttgttgaca gaagtatcta
     1921 atcatctacc tgcattaaca attgatccta cagaaagctt gtctaatcgt tttaatggaa
     1981 aacaggtctt tgttacagag gatagtcaaa ttatccaaga aaaaaattta actgagaaac
     2041 aaaaagaaat tctagaagat tatcgtctga tacaatatga tcttgttgca ggtaaccagt
     2101 acagtgcaac atgggcgaca caaaaaataa aagactaaca aataaactga gcaaggggtt
     2161 gtgataaaag acatgtcaca accccttgtt tatctcatta atttgcttgt ccataaacga
     2221 tttgattaaa gccatttttc aattcattat tcgctgtctc gttgaagtaa agataattat
     2281 cgggattccc ctgattttgt tgtgtcacca atacttttgg ctgattttgc tgaaacccga
     2341 atagatagac gtgctttttc agatatggtt ctgtctctgc atcggaatag acggctgtga
     2401 tttgatagtc tgcttgacct gtcccacttt ctgaccattc tgcagaaaca ggcactccac
     2461 caatagccat cttccactcc ccgttcaaaa tagcttgagg gactttaaga ccatataagt
     2521 ccacactcat ttgattcgta taacttttgt attgttgtcc cattgtttgg ccccatttgg
     2581 tcataaatgc ctctaattgt gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt
     2641 cgctgctttc tgaagaggaa gatgaagcct ctacagaaga acttgaagta gatgtttgag
     2701 ttgtttcagt cgttttggaa gactctgacg aagtagaggt atctgctgtt tctgtacttg
     2761 ctgcagttga cttgctctct gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg
     2821 tttcttttgt tgattctttt ttcgattcat tcccgttggt acaaccagct aacaaaaaag
     2881 ctagaccaat tgttactaaa atcctttttt tcaattaaaa aactcctttc gattactccc
     2941 acttctttat tctatcaaat aagcaatgtg attttattga tttttcgtat tttctcatca
     3001 atctttcaaa tcttctaaaa actgagaagc acccgctgat gttgtctgta atacatcagc
     3061 gggtgcttct tttattttaa tcttcgtgat tttccaattt acctcacgct ttatatttgt
     3121 atttgaagaa acatagactt ttagtcatca tcgtcttcct catccaaatc ttgtcggaca
     3181 gtttttcctt ggagtctttg tacttggtag atgatttctt tattgaaact taaaagttca
     3241 acaagcaatt gttgcaagcc atatttttct tctttttcag ctaatttgat tgcccgtgtg
     3301 ataaagacat tttggaaatc aaaatctttt gcaaagtttt ccaacattgt tgaggctggt
     3361 aaatatttgt tttgaccatg ttcgtaaatt tttccataac gcatgacttc ttctaaagta
     3421 gacggaacga tttcattttc aatcactaac tcatgagaga ttttattgaa ccatctcgct
     3481 tcttgatcag catattctga aaaaagggta cttaattctt gcgaagatgt acccaaaaca
     3541 tacaattgtg cttggatcaa tttcatttct tctaatttaa aatttgctaa tacatgcccc
     3601 accatggcgc cagcagttgg tttatgatgg tcgatatctg cttgttttgt ttcttgaaca
     3661 aatttttctt ccactatatt cattgattta cgcctctttt acttttgctg attggacttc
     3721 ataacctaat ttttccacaa ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa
     3781 gtttgctttg attttgctcg cattaaacaa aactttcgta ttgcttaccc caggctgttg
     3841 agaaaccgca tgttcgattt tttgcataca gcttggacaa gctagtgtat ctaattgcat
     3901 aataattttt ttcatcgtag ttcttccttc ctttttcttg atagtcttat tctagattac
     3961 agcttccata aaaaaattga ttcttatcaa gttttacgat ttttcttctt tgctttcgtg
     4021 tccaaaatac aataaacgca tcgcgttaaa gataacgatc aagatactag cttcgtgaac
     4081 gaacatccca ctagccatgt aaataaaccc ggcaaataag ccgttgagta aaaataagac
     4141 gacaccaatg gcaatcacta cattttcacg ggtatttaaa acagttttct tagccaagcg
     4201 gtaggcatga accaacgatt caaaattaga ttgcatcaag acaacgtcac tcgtttcgat
     4261 agcaacatcc gttcctgatc ccatagcaat cccgatatca gccgcagcca aagagggaga
     4321 atcattgatt ccatctccaa taaaagcaac gcgcaatcct tgttcttggt attttttcac
     4381 aaattgaacc ttttcgtctg gtaataattc agcatggact tcatcaatgc ctaacatatc
     4441 agctacataa tcagcagtca tctgattatc acctgtcagc atcactaact gttttgctcc
     4501 tgcttgtttc aacttagcta attgttcagc gacttctggt cgaatcacat ctgacacacc
     4561 tatgatttga gtgatttttt tgtcgattgc tacaataatg accgagctgc ctgtttgttg
     4621 taattgatgt aaatcttgta cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa
     4681 tttggtgttg cccgcacaaa ctgtatgacc atcaatctca gctgtgattc cttgtccctt
     4741 gattgtctga ttatctttga ctgctaattg ttgaaaatca atgtctttct cctgagcata
     4801 atggatcacg gcgcgcccca gaggatgatc ggataacgtt tctaatcgag ccactaaagc
     4861 taagctgtcc ccttcatcct ctgtatagtt cttgatagtg gatacagcag tagttccttt
     4921 ggtcaatgta ccagttttat caaagacaaa agtatcgatt ttggaaaaag tatccattgc
     4981 ttcaccgcct ttgactaaga tgccccgttt cgctccatta ccgattcctg ctacattcga
     5041 aacaggcgca ccaatgacta aagcgccagg acaaccaaga accaaaacag tgattgctaa
     5101 acgaaaatca cgactaatca gaccaacaac caacgcaata agtaaaacgg ttggagtata
     5161 atactgagaa aatcgatcaa tgaatttttc cgcatgggat ttggtatctt gtgcttcttc
     5221 tactaattcg ataattttgg caaaggtcgt atcatctccg accttagttg cttcaatcgt
     5281 cacgtagcca tctgctaaaa tcgttccaga gaagacttca tctccactga ttttgacgac
     5341 ttgtcgtgat tcaccagtca ctgcggcttc atccgcatac cctttcccgt caatgactcg
     5401 tccgtccaca ggaatagctc caccagtttt tactaagacg atatcgcctt cttccacgtc
     5461 atcgatttcc acttcttcag tcgttccatc ttcttggaca acactagcag tagatggggc
     5521 catctcggtc aaagccttga ttgactcccg cgttttggtc aatgtttttt gttccagata
     5581 gctaccaaac aaaaacaaga atgtaacgat agctgactcc ttgtattctc cgatcataaa
     5641 ggcaccaagc acagcaatag taacgagtaa atcaattgag ataactttga ctttcactgc
     5701 ctgatacgca tggatcataa taggtatgac cgcaataata gaagctatgg ctagagcaat
     5761 gttatagaac aagacaaaac cagtaaattt tccgataaaa ccaaaaataa tcaacacagc
     5821 tgataaggcg gtgatatgat ttcgatgctc ttggatccac ttttgtaatt tcatctttct
     5881 ccctcttttc ctttcattct tacgagacta agtatagata cttatccatc ataaaaaatt
     5941 gaccactatc aagtttagtc aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa
     6001 tagttagcaa gaagactcac taaaagtgat cttgccaact attgtcgttt ctatcgaaat
     6061 tttcattgaa tgattttcct gtacagcata catcggagat ctatttcttt atctaagatt
     6121 tttatcttgc taattttttc ctttagtctt tcaaatccac gtcgttcata gaattgatac
     6181 gtacagccac tatctgtaaa aaggaatatt tcttttcctt tctctctttt ttcaaattct
     6241 gttagcaact tggtaccgat gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg
     6301 atctctccat ctggcgaatg cttctttaaa tatgctgcaa gcatttgttc atttgctgtc
     6361 tcgtaagctc ctttactttc tttagcaaat atattttgta aaaaatcgaa tatcttaaca
     6421 tatatttgct gtccaaaaga acgatgtttc tttttttctc cttttatctc cgccagcaaa
     6481 acacctgcca actcgtcctc tgcatacaaa gcaagaattt gagttgctcg attcaactcc
     6541 atatacaaaa aatatcttcc gtaaagattc agcagaagtt cattttccat atatctctca
     6601 aagttcattc cttcaacagc aaaatagata gcttttttgt aatctttctt ttctaagggt
     6661 ttaaaagtta gtttcatcat ttcgcctcct caacttcaat gattgtgtta tacttacatt
     6721 ataatagaca cagtgtctac tatctataat caatcatcta taagtttgtc gattaagcga
     6781 cagctttgct ttgatatgtc ggataaggag ataggtaaat gaaaaagaca gatagacgcg
     6841 tcaaaaaaac agaaaaagct ttggctgaaa cactatccac attattagta aataaaaaga
     6901 ttcaagcaat cactatacgc gaactaacag aaacggcaga tgtacatcgt tcgacttttt
     6961 atacacatta taaggatatc tatgatctat atgatcaatt agaaagcaac ttttttcggg
     7021 atctaaatga aattctctct tatgatccag cccatagcta tgaggaacta tacactcgct
     7081 tgattgacta tgtttacgca aatacttttt tatatcgctt atttatcctc aaccatggtg
     7141 atatttcgtt tcagaagaaa gttaaacaag ttattgaaga aaactatctg aacatatggc
     7201 tatatgaaga caagaaaacg attatcacag aagaaatgag attctttact acgtaccacg
     7261 tccaaggttg tctatccgtt ctcaatcggt gggtagaaag caattacaag tactccaaaa
     7321 aagaattatt agaactctta cagaaactga atgatcacat agagaaaatc atgccttgaa
     7381 taaaaacata ctaagcttag ttaagaagaa ccgtaacctt ctcgtataac aactgaatct
     7441 tatttagcca aattgtttac tgaagatttt ttgagtaaaa tttctataat tcctacttat
     7501 tgaaaagtag tttgtagtgc tgcttctccc tctctaagtg atatatcttg ttgtaccaat
     7561 caaattcacc tcatcattaa taaattactt ttaggctaat ttattacaac ttataggttg
     7621 aagctgtaac caatttatag tcatattata tttttatcat ccagtttatt gaattcttct
     7681 cgtttacctc aattcatttt caaggggaat cagctcatca tctaacattt tacgccatgc
     7741 tcgcttcatt tcttgtgggt atttaaatgg tttcgcttct ggatgtttgg ctttatatcc
     7801 ttcgtaatcc ttactgttga taattgcttt catattagga attggatctt cacctggcat
     7861 atattgcaga ttagaagcat gtaatttttt ctcagaaaca aaccactcat tggaaaagac
     7921 atgaatcaaa tcatcactta cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc
     7981 attgctatct agtgtacctt catttctttg tgcttaaatt caaagataat tttctcaata
     8041 aagatttttg attccagtaa gattccttta ctccttttac aacatagcgt tgtcctttgt
     8101 tttgaaacat cttcttcccc tctttgccat taaaaatagc ccaattctct ataaagaaga
     8161 ttaagcttca ttattataat ataaagttta aatttggttt attacttact ttttcttatt
     8221 ctctagtaag ctaatattga aaaaaatgta atcagatcat tatttttata tgttgtaact
     8281 tcctgataag aaaactaatt aaaagaaaga ggtaactaca taaactaatt aaaaaagcta
     8341 acttcatagt tggaggataa atggaagaaa cttcaatgat tgtttctcct tgtgttcctt
     8401 caatcgacac tagtcctatt atcaatctct cttttttgtc catttacttt tgcgactgtt
     8461 ccccaataat caaatacagg aagcgttatt ttttgtcctt tagattcatg tactgtatag
     8521 actattttcg aatccatcac ttgataaggc atagtatgcc attccttgcc aattcttact
     8581 tcatgattct gcaaatgttc tttgtattgt aatgcttccg tgggtgcata gtcataaagt
     8641 cctccgggaa ggagtgtcgt gtaacgaata agattttctt gattcattac caagtgatct
     8701 ctctgagtga caactttatg ttcatagttt gcttttgttg cagcagatac agccaacagt
     8761 aataaaacaa ttgccgttat attaactact tttctttgct tagctgatgt gctcttcatg
     8821 aaaacgaacg ataaaatcaa cgagccaaac agtacttgaa agcctaagat tcgccaagga
     8881 aattgaatca tttgaatggg tgtattttgt aataaagacc atggaaaaaa tttggtggat
     8941 aagagccaag agactaccgc actaaggtaa atcacttttt ctttgcgatt aaatccttta
     9001 aaaaagaaga gtggacagat caaagaaata ataccaatga ttcctaccgt aaaggttgtc
     9061 aggtcattat tcatcgcacc aatcaaacta tccgagacat ttaatgcttg gtgttgtaag
     9121 gtcgttttat ctggctcatt aatcgattga accatgattt gtcggaacat cggataccaa
     9181 aagtatgctg ttaaaagaat cgtcattcct actgcctttg acaactccac aatcaaccgg
     9241 atgctttctt tttttcttgt ttcacttact acaaagccgc ctaagactag taagccaacg
     9301 gctattacac tcataaaagt ggaaagaaca tgtgtataaa tgagtaagct catactgact
     9361 gctaatggaa ttgcacatgg ctttttctca taaatcagtg cgtaagcata atacataact
     9421 atcggtataa ccgtaagtgc aatcgcttca gcaatagcag aacgatgata aatatcaatc
     9481 gtacggtacc cataaaatgt atataaacag ctaaatataa aagccgcatc gattcttttt
     9541 aaaaatttta gtccataatg aaacatcagc atgatagtac aaacattcaa tagccaaaca
     9601 tataagatat aactaacgat taaattatta cttatccaat aaaaaatgac tgccggaaaa
     9661 aaggtcaagt agggataaaa ataattcact ccgctcccat aatgattaaa cgtcgtaaaa
     9721 ttgattggac cagaaaaaat tgaagataac cctttgactc tagcaaaatg gaaggcaata
     9781 tcccccgatg tagaaatagt atgaacattt ctaaaataat tgattgaata aagtccagca
     9841 ataattgcta ggagaatgga caatattatt tttttcttta cttgcattac ttaattatat
     9901 actcctttca tggattgctt tatctagtat aacttaaatt ttaaataaat cgttatttat
     9961 ttttactctc acttaaaaaa gcactaaaat tataagagag ggaggtaatg gtctgaacac
    10021 caagaattgt tccaacatcg aattgcttaa ctaatgagtt caaagcttaa ttatgtggct
    10081 cttatattta ccgagcgtgg attgcgatct ctttagcgaa acaaacatct ttcataaaaa
    10141 aaacagccat atccgctttc ttggatatga ctatttacta ctactttact atctttcatt
    10201 cttcttgtct cacttatgat aaggcgctgc gtatcatgag tgaatgatat taggaaaact
    10261 tcataaaata aatcttttat acatttccac ttctagatgg tcattcatgt tagaagaatc
    10321 aaactatttt tttactgtcg taataatatt aaaacactag aacaaatcaa ttaaattcca
    10381 taaaatataa ataatagacc tagcatcatc tggtataaaa tggatgctag gtccgttata
    10441 tttactcaaa attgattcta cgaatagatc ttcttatcat ttctatttta gtagtcatgc
    10501 gttgttgttc ttctaaaggt ggtaatggta taagtagttt agataaattt gtaccattta
    10561 catttggttg cccagttccg taagaaattt tttctaataa cttccaatat aatggcgagt
    10621 ctaagaagca gtctacgtat tctgataata atttctcaac taacctaatt cgaatcaagt
    10681 aagaagcaaa aacagattcc tcacttattt ccttaaccaa gaaacttttt cccatagttc
    10741 ctcctgtcct tgctatcaaa atgtcatttt cttccaatct taaatcaact agtttagaat
    10801 tactaatatc acagtaaggc acactggacc aatttacacg tccctcttgt atatctgtga
    10861 ttctcaaaaa cttaacattg ccttgttttt tcgccgaatc tgtatagcca tattgaatct
    10921 tagtagaaat acttcctaaa taaacccata gtcgaacatc gctagatgat ttttcttgtc
    10981 gttctttatg aacaacagtg atcatcttga gttgtccgac tattttttag ttgcagcaat
    11041 ctttttggga cgcttatttg catgaagcaa cgttgctaag tctagtaata aaacattggc
    11101 taacacagga ttggctttct cgcgatacgc tgcggcgcca ctttcaaaca atactgctag
    11161 acgatcttct acaacaacga tttgttccaa atacggtgga aatttgattc gttttaggat
    11221 ccggctagga gagtcccgct cggacactaa gagttccgag gtataaggac cataagaacg
    11281 actggctaag agataatggc cataaaaagc cacaccttga atcttatcag atgtttttct
    11341 gctcattgtc acggttggtt ggattttgtc tttgcggcga attgctgttt tcatttcagc
    11401 tatcgtcggc aatagataga aagtcaactg tccttcacct tttagtgtga agttgccaga
    11461 taccagttga tcattgtttt gagtcaaata cgatgcttgt ggaatctctg agagatcagt
    11521 tgtcagccaa taagcgattg gctgtcccgt ctcttcagat gtatctgcta agatatcgat
    11581 caaacgtaaa gcggctactc gtccagtagc agatcctgtg attgtcaacc ataaaagttc
    11641 ccgttttgta tcatatacta agccccctac atgtggccgt ttaggtaaaa tcagcagttt
    11701 gatcggctgt cctgtttctc tatccagtac atgaataata gaatgatgct gatggtcata
    11761 gcagtaggca gaaataaaca caaaatcttg agaaaaagcc aacccttgcg gcgtcatcca
    11821 ttgacaaacc tcattttcgc cggtttccgg caccaccatt cttgtttctg ttaatccagg
    11881 aatgttatat tgttttgctt cagccttttc ccaagctgct tgaacaatag gataccgttt
    11941 taactttttt tcaatatcct gatgtatccg ttgctgcttc caatgcttaa actttaggac
    12001 atagtaaaca agaaggccaa tgaccagcaa cactaaaatc attattgcta cccgctgtgt
    12061 catttttttg cctccttgtt ctattcgatc aatcgtccat ataccgctat taattaccgt
    12121 aactacatgt ttcttttact agcgtttctt tataggatct ttcataatct catttatagt
    12181 aacattaaga atcccttatt ctactatagt cacttccaca tcaatattgc ctcttgttgc
    12241 tttagagtat gggcaaaatg cgtgggcttc ttctgcgatt ttctctgctt ggtccttctc
    12301 aagtcctggc aaagaaactt ctaaaacgac cccgatttgg tagcccccat cttctaattg
    12361 aaataaagat acccgtgctt tgacttcaga atcagactca agattatttt cttccagcat
    12421 gtgttgaacc gcgccgttga agcaagatgc gtatccagcc gcaaaaagtt gttccggatt
    12481 tgtcccttca ctatggattc ctgggcggtc gatcttcatg tgcatacttc cgtttggtgc
    12541 ctcaacttcg ccatcacggc ctccacgatt gatcattgct gtttcgtaca tttttttcat
    12601 tgagatcatc ctttctttcg tcgttctata ttaagtgtaa cgagtatttt ttaatctgac
    12661 aaaggtaatg cttgcattcg gcgacatcag ttagacaaca tcgatgatca gtgatagaag
    12721 cagtttatct atacaaaaaa agaagggttc tatgatattt ggtgtcctta ctcgccttga
    12781 gtaacaacac caaatatcat agaaccactt aatcagcttc acactgcttt ttctactttc
    12841 gtagttgaaa aaaacttaaa caaatcatcg gcaaatagtt aaagaaaaaa cttaatcgtt
    12901 gaaagaagcc caattgctta aagaaggcaa actgactaat cgacggaatc cgtgctaatc
    12961 cgtaaagacc tgcaattagt aaactaataa ctgttaaaac aagataaaaa tggctaagcg
    13021 taccttgacc actttgacga taaagcagaa caagtaacaa tgggaaaagt agaaagcctg
    13081 catagcctag cccagaacct atattatgga tccaagtcga tagattacaa gagctttcat
    13141 tcgtattgat actgaataag ccagtaaaaa tacaatcacc tatcccatat aaaccaactg
    13201 ttgcaacaac aagtattgca agtgtttttg atgttccaac aaataggtga tacaacgcag
    13261 gcaacgaaag aacaaaaaaa agtccagata caactgacca gaccaagaaa gcacgtctga
    13321 ctggactatc tacgtcacca aacacactaa ttacggtagt catttgattc agtttgggat
    13381 aaaaaaggcc aaggatatac ggtgtcgata aatcactcat cacacctaat aataaaaaat
    13441 aaaaaccata tttttgcaaa aaaatcattt tcttccccta ctttttagtg attattcgat
    13501 acatccaaca atcaaatttt ttctcatttt aacacatatt tttattctag ttgtcttttg
    13561 aacaatcaat aacataaagg tgagtttctt ctacatttta aaaaatcgaa tggcactgaa
    13621 gagttcagtt gtcagcatca atctcctact caaaaaggca aaaaaacgta agtcagatgt
    13681 tgttgacatc tcacttacgc atccattgat tgaaaatttc ttagtaaaat ctactagcct
    13741 aaaaatttga agacttcttc tcgatcatac agtttaaata ctttctcgca taaaaattga
    13801 gtaattagtg ccgcaaaaac agggatgact aaccaacaaa gaacaacaag aaagccattt
    13861 agccctgcat ctaaagaagc caacggacca acaagtccga ccacgccgaa accagcagaa
    13921 gcaggcgtac ctgaaataga aaatagagcg acaggaatag ctgtaatcac tgcactaact
    13981 aaacaaggaa taagaatcac aggttttcta aatagattag gcatcatcat cttcatagcg
    14041 cctaaagcaa tcgcaattgt cacacctgac ttattaacct tccaagagtt cactactaaa
    14101 acaaaagtgg ttgcagctac gcccatcgca gccgcaccag cagaaacacc atctagttgg
    14161 attgccattc cgattgctac tgttgaaata ggagagataa tcaaaaaggc aaaggaacaa
    14221 ccaatcaaga tagacattaa cagcggttgt aaggtagtga agttattaat caatgatcca
    14281 attgcagaag taattgctgt tacaaaaggg aataaataaa aaccaagtaa acctgcacct
    14341 acacctacaa caatcggtgt taacacaatc gacactgacc caaaactatt tccaatcagt
    14401 aaaatcagcc cgacagcaat cgatgctgtc agcatcgtat taataatatc gcctgtacca
    14461 gcaccaacgt aaacacccgt ttcacccaaa tcaggtgtga atttgactac accagaacca
    14521 acaaaagaag ctccggctac aaccatcatt ctcatcggat caaatccaaa ttgtaaggca
    14581 atcaaaccac caatgatcaa aggtgttgct agttgaaaaa taactgctaa ctgtgcgatc
    14641 gttaccgcaa agggatacgc actaaaatat tttaaaatgg cacctaatac tgcattagga
    14701 atcaatccaa taatgattcc tgttgctgta ccagcgagga ctttattcag aaaaattttc
    14761 ggtgatattt tttgtgaagt ttccattcaa tctctccttt attttacgtt caaactatct
    14821 tcttttgcat ggacaagctc agtcaagaaa tcacaataag gcgtcgcaac accatatttc
    14881 ttcccttttc tagaaattgc gccattgata taatcgattt ctgttaatcg atggttctta
    14941 atcaagtctt gatacattga aggataatgt aacccaattg tttccgggtc aaaacaagat
    15001 tcacaatgtg caatgacttc tgggacatct agttcaatct tctctactgc tgctactttg
    15061 gcaaactcat tgacaatcgt cgccaccatt ttatgagcag tcgatgtttt tcctagctca
    15121 gccatattaa cgtctaagat tgtgcatagt ccgttcattg ttccgttcac acatgctttg
    15181 cgataaatag aatagtgaat attgtcagaa aaatgagcat ttaatcctga ttcagacaat
    15241 ttgtctgcta atttttttgc agctgcttct tttccatcac ctaaattttg taattctact
    15301 gaaccacttc caaataattt gacctgacca ggaccttcaa gacctgccgt ccacatggta
    15361 tttccaatat agatattttc cattggtaca aatttctcaa taatatcttc atgcccgata
    15421 ccatttaata gacataagac ctctgtatct tttttgatta atgattggat atcctgcagc
    15481 attttttcca gctgcatcgc tttggtaaat agaataatca gatcaacttg atcttctttt
    15541 tctacttcgg attgaagaac gattggtagt tttgcttcta cctctttacc attaaaattt
    15601 gcttgtaatc catgttcttt gatttgttgg acatgttctg cccagccatc gattaataat
    15661 acttcgttcc cactttggtg aagcatcaac ccaaagcgac ttcccattgc gccagcgcct
    15721 gcaattgcta ttttcattga ttcttcctcc tttttctttc tattatactc atttgagaat
    15781 cttttcacaa cctcgttttg ttattcttct cttatttatc atgtttatat ccaccattca
    15841 agttatttta tcagcaaatc aataaaaact ggaatagaat cccaatttca tcgtaagaga
    15901 aactagacta gcaataagaa aatagaaaca ttcatagaaa aaatctcact cctctgatta
    15961 taaaagaggg ggcattaacc tcgtgtatac taaaaccttc cattcattcg atggatgatt
    16021 ttctttttgt gaaaaaatat tcattgtagt acgatgttgc gcaacacaga cggatggtat
    16081 tgcttattat ttattaaaat cgaacaatac acttatttaa aaacttcttt cccagatttt
    16141 cttccatttt tacagttata actagtatta tcatgaaaaa tagcctttta ttgtctatgg
    16201 gaataataat ttaaaattgg tactttttta ttaattagtt ca
//
LOCUS       DAZAOG010000053        16097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000053 DAZAOG010000000
VERSION     DAZAOG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.053"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHDLD_001425"
     CDS             226..894
                     /locus_tag="ACHDLD_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN3363132.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACHDLD_001426"
     CDS             complement(1094..1480)
                     /locus_tag="ACHDLD_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363133.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACHDLD_001427"
     CDS             complement(1452..2834)
                     /locus_tag="ACHDLD_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3363134.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACHDLD_001428"
     CDS             complement(2990..4177)
                     /locus_tag="ACHDLD_001428"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN3363135.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACHDLD_001429"
     CDS             complement(4184..5215)
                     /locus_tag="ACHDLD_001429"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3363136.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACHDLD_001430"
     CDS             complement(5231..6685)
                     /locus_tag="ACHDLD_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN3363137.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACHDLD_001431"
     CDS             6860..7765
                     /locus_tag="ACHDLD_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN3363138.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACHDLD_001432"
     CDS             7856..8533
                     /locus_tag="ACHDLD_001432"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3363139.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACHDLD_001433"
     CDS             8530..8700
                     /locus_tag="ACHDLD_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363140.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACHDLD_001434"
     CDS             8814..9710
                     /locus_tag="ACHDLD_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3363141.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACHDLD_001435"
     CDS             complement(9765..11135)
                     /locus_tag="ACHDLD_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3363142.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACHDLD_001436"
     CDS             11373..12119
                     /locus_tag="ACHDLD_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3363143.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACHDLD_001437"
     CDS             12116..12955
                     /locus_tag="ACHDLD_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3363144.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACHDLD_001438"
     CDS             12956..13906
                     /locus_tag="ACHDLD_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN3363145.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACHDLD_001439"
     CDS             14047..14799
                     /locus_tag="ACHDLD_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363146.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACHDLD_001440"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACHDLD_001440"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN3363147.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5220 a         3009 c         3233 g         4635 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatg
//
LOCUS       DAZAOG010000054        15955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000054 DAZAOG010000000
VERSION     DAZAOG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.054"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(391..813)
                     /locus_tag="ACHDLD_001441"
     CDS             complement(391..813)
                     /locus_tag="ACHDLD_001441"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN3363148.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(827..2233)
                     /gene="atpD"
                     /locus_tag="ACHDLD_001442"
     CDS             complement(827..2233)
                     /gene="atpD"
                     /locus_tag="ACHDLD_001442"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN3363149.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2256..3158)
                     /locus_tag="ACHDLD_001443"
     CDS             complement(2256..3158)
                     /locus_tag="ACHDLD_001443"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN3363150.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3172..4728)
                     /gene="atpA"
                     /locus_tag="ACHDLD_001444"
     CDS             complement(3172..4728)
                     /gene="atpA"
                     /locus_tag="ACHDLD_001444"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN3363151.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4751..5293)
                     /locus_tag="ACHDLD_001445"
     CDS             complement(4751..5293)
                     /locus_tag="ACHDLD_001445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN3363152.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5280..5804)
                     /gene="atpF"
                     /locus_tag="ACHDLD_001446"
     CDS             complement(5280..5804)
                     /gene="atpF"
                     /locus_tag="ACHDLD_001446"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN3363153.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5895..6110)
                     /gene="atpE"
                     /locus_tag="ACHDLD_001447"
     CDS             complement(5895..6110)
                     /gene="atpE"
                     /locus_tag="ACHDLD_001447"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN3363154.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6163..6882)
                     /gene="atpB"
                     /locus_tag="ACHDLD_001448"
     CDS             complement(6163..6882)
                     /gene="atpB"
                     /locus_tag="ACHDLD_001448"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN3363155.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7184..7648)
                     /gene="smpB"
                     /locus_tag="ACHDLD_001449"
     CDS             complement(7184..7648)
                     /gene="smpB"
                     /locus_tag="ACHDLD_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN3363156.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7670..10027)
                     /gene="rnr"
                     /locus_tag="ACHDLD_001450"
     CDS             complement(7670..10027)
                     /gene="rnr"
                     /locus_tag="ACHDLD_001450"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN3363157.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10030..10785)
                     /locus_tag="ACHDLD_001451"
     CDS             complement(10030..10785)
                     /locus_tag="ACHDLD_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3363158.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10888..11124)
                     /gene="secG"
                     /locus_tag="ACHDLD_001452"
     CDS             complement(10888..11124)
                     /gene="secG"
                     /locus_tag="ACHDLD_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN3363159.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11242..11520)
                     /locus_tag="ACHDLD_001453"
     CDS             complement(11242..11520)
                     /locus_tag="ACHDLD_001453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363160.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11633..12601)
                     /locus_tag="ACHDLD_001454"
     CDS             complement(11633..12601)
                     /locus_tag="ACHDLD_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3363161.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12816..12860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12884..13858
                     /locus_tag="ACHDLD_001455"
     CDS             12884..13858
                     /locus_tag="ACHDLD_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFN3363162.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13863..14438
                     /locus_tag="ACHDLD_001456"
     CDS             13863..14438
                     /locus_tag="ACHDLD_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN3363163.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14505..15644)
                     /locus_tag="ACHDLD_001457"
     CDS             complement(14505..15644)
                     /locus_tag="ACHDLD_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN3363164.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4360 a         3443 c         2856 g         5296 t
ORIGIN      
        1 aaatctgagc cagaatgtgg ttcattctga ctcagatttg attttgcaca actcatcagt
       61 ccttattgac aaagagccca aaagtcttgt cgcagtccct tattccaaat aaacggtgtt
      121 caatcatctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttcca
      181 tttgtttctc taatactcag gacaaaacgc tgatttcaca acctccgata tacggtgttc
      241 cagaagtagc ttcttcgaaa aataagctga gatttcacaa aaatttgaga agcaattttc
      301 gtaaattcct tcttatgtct tgaagctgac cacttctgtc ataacctctt tttactgttc
      361 ttaaacacgg ataaacatcc gcatctcatt ttatgagtgc ttagatactt tgatacgatt
      421 gattgctcgg tgcaacgcaa cagttgcccg tttcaattcg ttcgtatctt gttttgcttt
      481 tgcttcttcg atttggcgtt ctgcgcgttg ttttgcacgt tccgcacgag atacatcgat
      541 atcacgttca cgttccgcac tatcagcaac gattgaaaca acattatcac gaacttccat
      601 gattccgcca ttaacagcga tccaatcgac gtgggtatct gaatctgttc gttttacacg
      661 aacttcatca atcgtcaatg gaacgatgat tggtgcatgt ttaggcaaaa tcccaagctc
      721 accagctgtc gtacgtgcaa cgacgatagc tgcatggtga tcatagacca aaccggcagg
      781 agttaccaca tttactgtta aatattgatc catggggcac ccctttctag tagcctagtg
      841 ttttcgcttt ttctacgacg tcttcgattc ggccaacact tcggaaagct tcttcaggaa
      901 gatcatcgta tttgccttcc aagatttcgc ggaatccttt cactgtttct tctacaggga
      961 catatgaacc tggaagacca gtaaattgtt cagccacgtt aaagttttgt gacaagaaga
     1021 attggactct gcgggcacga gaaactaaca ctttttcttg atcggataat tcatccatac
     1081 ctaagatagc aatgatatct tgtaattcac gatatcgttg caatacgtgt tggacttctg
     1141 ttgctacttt ataatgttct tctcccacga tttcaggtgc taaagcactt gaagaagaag
     1201 ccaatggatc taccgctgga taaatccctt gttctgttaa tttacgttcc aagttggttg
     1261 tcgcatccaa atgggcgaat gctgttgccg gcgccgggtc agtgtagtca tcggctggca
     1321 catagattgc ttggatcgat gtgatcgaac cttttttcgt agatgtgata cgttcttgca
     1381 attgacccat ttctgttgcc aatgttggtt gataaccaac cgcagaaggc atccgtccta
     1441 acaaggctga tacttcagat ccagcttgag tgaaacggaa aatgttgtcg ataaacaaca
     1501 gtacatcttg accttctaca tcacggaaat attccgcaat cgtcaaacca gtcaaagcaa
     1561 cacgcatacg tgcacctggt ggttcgttca tttgtccgaa caccatggct gttttttcga
     1621 taactcctga atctttcatt tcataataca agtcattacc ttcacgagta cgttcaccta
     1681 caccggtaaa tacagaaata ccaccatgtt cttgggcgat attatggatc agttcttgga
     1741 tcagtacggt tttaccaaca ccggcaccac cgaatagtcc gactttccca ccttttaaat
     1801 atggggcaag caaatcgata actttgatcc ctgtttccaa aatttctgta cttgtactta
     1861 attcgtcaaa ggctggtgct tttttatgaa tttcgcttct ttccgcatct tctgggaaag
     1921 gtgtttctaa atcgatcgta tctcctagta cgttgaatac gcgacctaat gtttctttac
     1981 ctacaggaac agagattgct ttgcctgtat caatgacttc cattcctctt tgtaaaccat
     2041 ctgttgattc catcgcaatg gtccggatca ctccgtcccc taattctaac gctgcttcaa
     2101 gaacaacttt tgatttattt tcgtcatttt tataaacaac taatgcgttg ttgatatctg
     2161 gtaaggattg gtctaaagaa aattccacgt cgacaacggg accgattact tgaacaatct
     2221 tgcctgaact catctttttt cctccaatct caatactatt ctagtgccga agctccggca
     2281 acaatttcgg taatttcttg ggtaatagcc ccttgtcttg cacggttgta agaaaccgtc
     2341 agctcatcaa tgatattagc agcattatca gttgctgttt tcattgctgt cataccagca
     2401 gcatgttctg ctgttttggc gtcaacgatc gcaccgtaga tcaaactttc agcatactga
     2461 ggcaataact gagacaagat ttcttctttt gaaggttcaa aaatatattc ttgttcgaaa
     2521 gtagttgctt cttctggatc taagtctgag attggcagca ttttttctac acgaaactga
     2581 ctagttagcg aattgatatg atggttataa catacgtata attcatcgaa tacttcgttt
     2641 tggtacatag ttgttgccat atttacgatt ttgcggactt catcaaagct tggctgatca
     2701 gatagattgc gcagctcata tgctagattg ataccgcgtg ctttaaagaa gtctgcacca
     2761 gttccgccaa ttgcgatcat cacgtaatca tcgggtgatt ggtggtcttc ttctaaaata
     2821 gacatcgttt gtttcaatat cgagctgtta tatccaccga ctaaaccgcc atctgcagtg
     2881 atgacgatat aacccgtttt cttaacagga cggctgagca acatgctgtt gtaattgata
     2941 tcccctctag gattttctga ggcaatatca tttaactgag tagcagtcaa atgcgtaacg
     3001 atttctctta cttttgttgc ataaacctga aacttctgtg aagaagcttc agattttgtc
     3061 aacttagaag cagacaccat ctgcattgcc cgcgtgattt ggctcgtttt tttcgttgaa
     3121 gcgatacgtg tcttgatttc atttaatgaa gcacccattc atctcacctc actatgcatt
     3181 ttgaatggat ttcaatttat cttctgtaga ggaattattt ccttttgtag ctaagaacat
     3241 gtctttatat tcctgaatcg cactgttcaa cgcttcttct tctggaagat cttttgttgt
     3301 acggattgtt tcaaagatat ctgaatgatt ggtatccaga tattcgaata attcatgttc
     3361 gaagtctaaa atactgtcta cagggatact atctaagaat ccatgtgtca aagcatacaa
     3421 aatcactact tgtttttcaa cagctagcgg tgcatgcaat ttttgtttca agatttctac
     3481 tgtacggcgt ccacgattta gttttgcttg tgttgctgca tccaagtcag aaccgaattg
     3541 tgtaaatgct tctaattcac ggtaacttgc taagtctaga cgtaaagtcc cagcaacttt
     3601 tttcattgct ttgatttgcg cagaaccccc tacacgagat acagatagtc cggcatcaac
     3661 cgctggacga gtacctgcgt agaacaagtc gctttctaag aagatttgtc catcagtgat
     3721 cgaaataacg tttgttggga tataagcaga gatatctcca gcttgtgttt caacaaatgg
     3781 taaggcagtc attgaaccgc cacctaaatc atcacttaat tttgctgcac gttccaataa
     3841 gcgtgagtgt aagtagaaaa catcccctgg ataagcttca cgacctggtg gacggcgaag
     3901 cagcaaggaa agttcacgat aagcaacagc ttgttttgaa agatcatcaa aaatgatcaa
     3961 gacatgtttc ccgttgtaca tgaactcttc acccattgca gtaccagcat atggtgcgat
     4021 ataaagcaac ggcgctggtt gagacgcacc ggcattcaca acgattgtgt aatccattgc
     4081 gccatatttt ttcaatgttt caacttgtgt acgaactgta gaatcttttt gtccgattgc
     4141 tacatagata cagatcatat cttgaccttt ttggttgatg atcgtatcga tcgcaatgga
     4201 agttttccct gttttacggt caccgataac taattcacgt tggccgcgtc cgattggtac
     4261 aagagcgtcg attgctttta gacctgtttg cattggttcg ttaacagatt tacgttgcat
     4321 aacgcctggt gccattgctt ctactggacg agctttgtca gtaacgattt cacctagtcc
     4381 gtcaattggc tgacctagcg gattaactac ccgtccaatc aaggcctctc caactggaac
     4441 ttccatgatc tttcctgttc gttttacttt atctccttca cgaatggact cgaaatcgcc
     4501 aaggataatg atacctacat catttgattc taagttttgc gccattccgt atgagccatt
     4561 tgaaaattca agcaactcac cactcatcgc gttttctaaa ccatgtgcac gagcgatccc
     4621 gtcacctaca taagttacgg taccgatttc ttcgactgaa agcacgtttt gataattttc
     4681 aatttgttct ttaatcaagg cactaatttc ttctgctttg atggccattc gattcaccta
     4741 cctctttgta ctatctattt aattgattac gcatattttc taattgcgtg cgaatgcttc
     4801 cgtcgattac tcgatggtta gcttcaacaa tcgcgccacc aataatagaa gaatcaacga
     4861 tttgttttaa ttcgactgat tgatagttca ttgtttttgc aacattcttt tctaattgta
     4921 ttcgttgttc gtctgaaagt ggaactgctg ttgtgacact tcccaacagc aaaccttgat
     4981 gttcatcata tcgacgttca tattcttcga tcatgaacaa aagatcatcc attcggttat
     5041 acgcataaac aacttctaaa aagtttttaa cgattccatc atagccacta acaagcttgt
     5101 ccatgatttc tcgtttttca tgaggttcta gacgaacatc acttaacata ttacctaatc
     5161 caggtacttg ttcatagatt tgacgtaagc ttacgagatc ttgataaatt tcttctgctt
     5221 tatttgaatc gatcgccagt tcaaataaag ctttcccgta acgtcttcct actgtatatt
     5281 tatctagttt catttgaaga acctagacct tcgatatatt gattgattaa tgattcgtgc
     5341 atttctggag ataattcttt gttcaagatt ttttccgcga tttgaagaga tagttctgca
     5401 acatcgtctt ttacagagtt cagcgctgaa tcacgttcta cagtgatatc agcttgggct
     5461 ttacttttta gacgagctgc ttcttcttga gcatccttca aaatattttg gcggcttaat
     5521 tctccacttt ctttcgcatt tttgatgata tcagctgcat cagaacgaga ggctaacaat
     5581 tgttgttcgc gttcttgttc cattttcgct gagttgatgc gagattgttc tgcagaatcc
     5641 aaatcattgg cgatcttgtc ttcacgtttt ttcaaaatat cgctgattgg tccccaagca
     5701 aaatgcttca agagagccag tagtatcaaa aatgagccgc tgacaacgat gacatttcct
     5761 aacatcgcat tgccaacttc tgcaattgcc aattgattca gcataggcga gccttccttt
     5821 ctttgaagta cttctagttt tcatgtaaaa aagcagtaac aaaccaaatt gtttctgcct
     5881 taatactgat tcaattaaac accaaaaact agaatcaagg cgacaactac ccctaagata
     5941 ggaacagctt cgactagagc taccccgata aacattgttg tacggaattg accagacatt
     6001 tcaggttggc gagccattga ttcgattgtt ttagagataa cttgaccgtt accataaccg
     6061 gctccgattg ctgctcccat gattgcgatt gctgctgcga tataattcat aattgttttc
     6121 ctcctaaatt agataaatag tagtttgttt ttaaaacgaa tattattctt ccacttcaat
     6181 cttatggctc atataaacca tagataaagt aacgaagaca aatgcttgga tgcttccgat
     6241 aaacaaggag aatgcgatcc agaccatctc taatggaatt gctaacggca atgagaacca
     6301 gcctaagctt attaccattt ttgcaatcaa ggttagcaaa acttcccccg cataaatatt
     6361 cccataaaga cgtaaagcaa gtgttaataa gtttgtaaat tcttcaatca gtttcatcgg
     6421 aaacataaaa ctatatggtt tcaagaacga atttacgaaa tatcctttga agcctaactt
     6481 tttcacccca aataagtgag taagtaagat cattataaat gccaaagtta aagtaacaaa
     6541 cgggtctgct gtcgggcttt tccaaagtgt tgtttcgtta ggtaaaacga tttttgttac
     6601 taaaccgatg ttgtttgcaa caaaaacaaa taaaaacaat gtaaatgcca acaaatggaa
     6661 attgctcaat tcttttctag gcagattatc tgttacgatt cctcgactaa aatcaatgac
     6721 ccattctatc gcattttgtt tgcctgttgg tttcaactta agatttcttg taaaaaagta
     6781 agctaagaag aaaacaatca aacaggtcag taacaccatt atacaaactg taccatcaaa
     6841 ccaaattggt ccaatgtgga acgttagcga gcgttcatcc aatccatttc acctcttttc
     6901 cccgaaattc acataaaatc aattcatgtg cgtatctggt tttttccaat cggtttctaa
     6961 ccacacggta tcataccacc atgaaacaga aaattcaaaa cattttcatg aaaattagac
     7021 acttttttat ttcagtcaaa aacgaagcga atttttcttt atacacggtg ctcctgatct
     7081 aagaaaaaac ctatgataat catgttttat cagattaaat gctaacaaat tttatcatta
     7141 agtaaaagca aaagcaccga atatgcgaaa aaggataatg aaattatcta gagaaatttt
     7201 ttaatgttcg atcaatttgt cgatcgatat cttttcgttt caaatcttcc cgtttatcat
     7261 aagatttttt ccctttcgcc agaccaatca ataccttggc atagccgtta cgaatataga
     7321 cttttaaagg aacaacagtg attccagtat ttttcaattc attttcaagc cggatgattt
     7381 gttttttgtg taataataac tttctcgtgc gcaacggatc atgattaaaa atatttcctt
     7441 gttcataagg actgatatga acattatgca agaaagcttc tccgttgcgg acgcgaataa
     7501 atccatcttt tagattgatc cggctgtttc gtatcgactt gatctcggtt ccttgcaaga
     7561 ccatccctgc ttccatcgtg tcgatgatcg aataatcatg gcgagctttc ttgttttgtg
     7621 caattaattt tccctcgcct tttggcatag ctgtttcctc caactcattt tattttttct
     7681 ttttcttctt tttaacacct ttgtaaaatg gcttcttccc ttttttctta ccgaacgttt
     7741 gcttactatc gctatatcgt ttgttcttct tgccatttct ttctgggcgt tttcctgctt
     7801 gttttccttc gcgttttcct gattttccat ttcgtgtttt tggccgttcc aacgctgtaa
     7861 cctcttctgc ggaaacaagt tcaaaatcta ctgctcgttc atctggatca gctttgacta
     7921 cttttactcg cactttctgt ccgattttaa aagtcagacc agttctttca cctacaagag
     7981 ctaaatgatt ttcaacaaaa tggaagtagt cctgtttcaa ttcattgata tggatcaatc
     8041 cctctactgt attcggcagt tcgataaaca ggccgaattt cacaacagag ctgatgatcc
     8101 catcgaattc ttcccccact ttgtccatca tgaattctgc tttcttcatg gaatctactt
     8161 cacgttctgt ttctactgca cgacgttcca tcttagaact atgatctgca atttcaggaa
     8221 gttccgtagc ccacgaactt tgattgcttt cacttttatc ttcactgtaa gaacggatca
     8281 gacgatggac gattagatcg ggatagcgtc gaataggaga tgtaaagtgt gtgtagtaat
     8341 ctgccgctaa gccataatgc ccaaaattgt cttctgaata gcgtgcttgt tgcatgcttc
     8401 ttagcaacat agtattgata actgcagctt ctggtttgtc ttctactcct tcgatcacat
     8461 gctgtaaatc ttttggtgtg atcgttcctt tcgttcctcg gaccagaatg cccaaagctg
     8521 aagcaaaatc aaagaagcgc tgcatttttt cttcttttgg ctgttcatgg attcggtaaa
     8581 taaacggcag atttaattta ttataatgtt cagctaccgt ttcattggcc gcaagcatga
     8641 aagattcgat caagcgttca ccaattcctc tgcttcttag ctggatatca tttgggtgtc
     8701 cttcagcatc aacaaggatt tttgcttcgc gatcctcaaa agaaatcgcg ccacgtttga
     8761 ttcgcatcgt ttcaaggatt cgatgcaatt cacccatttc ttggaacata ggtacgagct
     8821 gagcatagcg ttgcatcgtt tcttcgtctt gatcttctaa gatttgattg acagctgtgt
     8881 aggtcatccg ttctgttgtt tggatcacac ttgggaaaat atcatgagag actacttgtc
     8941 catttggatc aatctccatt tcgcaactca tggtcagcct tggaacatgg ggattcagtg
     9001 aacagatacc attcgataaa cgttgcggga tcatcggaat cactcgatca gtcaaatata
     9061 cacttgtccc tctttcatat gcttctctgt ccaattgact gccttgtgtc acatagtaag
     9121 aaacatctgc aatgtgtact ccaagaaaat aattaccgtt atctaatttt ttaaccgtaa
     9181 ctgcatcatc tagatctttt gcatcttctc catcaatcgt aacaatgagc tgatttctta
     9241 aatctctacg tccagttata tccttttcgc tgatttgatc tggaacttca tctgcttcag
     9301 acaatacatc atcaggaaat ttcgtaggga taccattagc cacgacaatt gagaggatat
     9361 ccatgccagg gtcattctta tgaccaatca cttgcttgat tatcccttct aaactagtcg
     9421 catacccttt ttcagggtaa tgggtcagtt cgatcagcac aatacttccg tcaactggac
     9481 gaactccctc tgctgctgca tatactttaa attggttgag ctttctatct ttcgggataa
     9541 caacgccgta aagatccgtt tcttgtattt cttcttctga ataagcaacg aattcccctg
     9601 ctaactgagt agtcgcccgg tctcgaatct cgactacttt cccctctgct ccgcgatcca
     9661 tatctgtatt tgctcgcttc atgatatcga ttgctacggt gtctccatcc attgcaaaac
     9721 cagtcgcttc ttttgggata tagatgtcat cttcttcggg atcaatcgtt acaaatccaa
     9781 atcctcgttc atttgctcga aatgttcctt cgatcaagat tggttttaaa ggaagtttca
     9841 cttttccttt tttattgaaa actatactct gttctctttc catttgagca atcgtttgta
     9901 ctagtaattt aaaatcatcg cttttttgga accctaaacc ttcagcgatt tcttccatag
     9961 aaaaactgcg tttgcgactg ctttccatga aaaagagaat tttctctttt atcgtttctt
    10021 ttgtcatatt tattcctcat tccaaggtaa tttttctaaa aattccaaga cgtctttttc
    10081 taattcgcgt cttgctgtac caactgtaat cacatgacca ctttcaggat accatcgaag
    10141 tgtcactcgc tgacggctca acttgcgtgc agtttcaaat acgccattag ggtcgatcat
    10201 ttcatctttc ccagcttgag ctaaaaatat cggctgttgg atatctgaca acctactttc
    10261 tacgattgct gcctgatctt gtatatccat taattgttgt tcagctaacg agcggtaagc
    10321 aactgctttt tcattgattt ctttctctga tttacctgct gtcttcagca cttgacgtac
    10381 gtatttttca aagttttcag gcacgtttgt ttttacagga gacaaaggcg aacagaaaaa
    10441 accgccgcca atcactgatt cttcttctag tgctcgtaca gcaaagatgc cgcccattga
    10501 taatcctaat acggcaatat cagaaaactg ctcagactgt aaaaaatgga tggctttttt
    10561 agtgtctgcc caccagcttt ctgctttttc ttgcaaaata tcatatggct ccattgttcc
    10621 gtgtccttta aaaagcggcg cataaactgt ataatctgct ttttccaaaa atcgtgcgag
    10681 cattcgaaca tcgtttggac ttcctgagta agcgtgaagc agcaaaaccg ctcgctttcc
    10741 atgttttgca taaaggggtt ttggtaattg tctcactcgt ctcacctctt tcgcctcatt
    10801 ttaccatagt ttctttcata acttcttctt aagcgttctc tttgcaataa aaaaagccgc
    10861 ccgccgtttt gcgagaggct tattcaacta cttcgaagat aaatatgcta ggacaaataa
    10921 taagatcatc cagatagctc ccataactgc agttgatcgc tgcatgacag cttcaaagcc
    10981 gcgagctttt tgtttgccga atagttgatc cgcaccgccg gtaaatgcac tcgctgcact
    11041 attttgcttg cttggctgca tcataatcgc aatgaccata acaatcgata aaacgatcac
    11101 gattcccaaa attaaattat acatatcgca gcctccttcc caaaagatat cgtctcttta
    11161 actttatcat agataaaagt ccgtgacaac tctatataaa tggaataaca gctgaaagtt
    11221 caaaactttc agctgttatt cttatgactg acgtaagacg tcttgatatt ctggtttctt
    11281 ttcaaactct ttttttgcat aagggcataa aggaatgatc tttttcccgt ctcgtcttgc
    11341 taattctact ccagtataca ctagcttttc agctaacttt tgaccacgat atgctggatc
    11401 tacaaatgta tggtcaatga tcatgatgtc tgggcccgca tctgaccatg tcatttctcc
    11461 gatctcttgt tgttcatcat tgaataaagc aagacgattt ttttcttctt tgatttccat
    11521 atgccgcact cctcaaataa ctgatatttt tttattccta ccttatttta gcagtctcct
    11581 gatctataaa caattattta tcctcgaatt acttacaaaa aagaagagaa aattaattca
    11641 tcgttattct ttgttcaata aatgaaaaaa tcactgtcca ataggttttc ggatctttca
    11701 tcgatgcttc caagtgttct gcttctggaa caagcagcag tgctttatcc cctgccgtag
    11761 cattttgaat cgtgtacgct gcttctgtag ggacaaaatg atctctttca ccatgaatga
    11821 ccaataaagg cagatggttg ctgccaagct gtctcgtagc tgatgcttgc tttaaggagt
    11881 aaccggcgta tttctgggca taatgattcg catatctcat gaccattttt ttaggaaaga
    11941 cagtgatttt acttaacata tatcggaatt ctgcataaac agaaacatat ccgctgtcta
    12001 aaataaatcc tttgaccgct gatggaagtt tttcaccact tgccatcatg atcgtagaag
    12061 ctcccattga tccgccgtgt aaaataatgg acgcattcgg ctgttcatct aagatgagtt
    12121 ggatccaggc aattaaatct agccgatcta gccatcccat tccgatgatt tctccttcac
    12181 tttctccgtg cgcacgtaaa tcaggtacaa gaacgttgaa tcctttctct gcatatcttt
    12241 tacctatatg agacatgtct cttttccctg tgcttcggta atcatgtacg caaatgaccc
    12301 atttattctg cttatttgga tggatgaaca tctgaccagc tagtttcaac ccgtcataac
    12361 ttttgattgt tttgggtgtt ccctgctccc aaaagctttc acactcttct tctaattttt
    12421 gtcgtttttt ttctactgac gtgactgcag cagggtccat caaacgatga ccagtgtctt
    12481 cataccagtc atatcctctc tgtactcccg ctcttactaa tttctttgca taaaaataag
    12541 aaagaaaatg gtaactgatc cctataatta tcagaataag aatgaaataa aaaataacca
    12601 ttgtacaccc gccgctttct gccaaactat aatctaacta ttatccaatg taccgatcca
    12661 taggattctt tattttcggc acacgtaaat tccatttctt tttcattgta tcatttactt
    12721 aaagttctcc tcaatagatt tacttgtaag agagcattta cattcgcaat aaaagccagt
    12781 atactaattt cgtgcattat aatcaagacg ctcgtcttgg actggttcgg aaacttccca
    12841 gaataaaaaa ctaagtatct ctaaagaaag gagattggaa ataatgcgaa aagtaattat
    12901 tgattgcgat ccagggatcg acgacacttt agctttatca ttagctgtca aatcccctga
    12961 tatcgaagta gttgctatta ccgtagtttg tgggaatgtc cctgcagata taggtacgca
    13021 gaatgtgctg aaatgcctgg aacgctgtga tcgtttagac attcctgttt atcaaggaat
    13081 ggaaaaacct ttgaagcaac ctttcatcag tgcacaagac actcatggta tggatggttt
    13141 aggggaaaca aatttcccga tgatcttgag aaaacaggca gaacctctcc atgccgttga
    13201 ttttttggct gattatttca aagaaaagac aaatacatca gtcattgctt tgggtccttt
    13261 gaccaatatc gcttttgctt tgaaagtcaa cccaaatatt gggaaacata tggatcgttt
    13321 tgtctcgatg ggtggaactt acaaaagcca tggtaactgt tcgccagttg ctgaatacaa
    13381 ttactggtgt gatcctgaag cagcttctta tgtattcgag aatctcaaac aaacgataga
    13441 aatggttggg ttagatgtga caagagaaat cgtcttgaca ccgactatcc tcgaatactg
    13501 ctgtcaaatg aatcctgaag agggagagta tctaaaagcg atcactcgtt tttattttga
    13561 cttccactgg aaacaagaaa gaattttagg atgtgtcatc aatgatccgt tagctgttgc
    13621 ttattttatc gagggggcca tctgtgaagg tttcaaaagc ttcactgctg tggaaactca
    13681 aggaataagt cgtggacaaa cattggttga tcgttatgaa ttttggcaga aacctgctaa
    13741 cagtaaaatt atgacaaaag tagatactcg tctttttttc cgaaagtttt taaccgttct
    13801 tttaaatgca caagaagaaa cgattatgaa agatttggaa aggttaaaga tgggctaaca
    13861 aaatgaataa aaacaaaatg gcaactcgcg tacttacagt gatggctttg tgtattggga
    13921 tcaattatat tggaggaacg attgctttat ggcttcgttt gccaatctat ctcgattcta
    13981 tcggaacgat ttttgctggt gcattgcttg ggcctgtacc tggaatgctg acaggattgt
    14041 ccagcggtat tttaagtgga gtgactacag atattttttc tctgtattat tcgccaatcc
    14101 aaattctcac aggcttgctt gctggattga ttttgccaag atatctcggc aaagataaaa
    14161 acagcttgaa actgttatta ctcgctttga ttttgtcggt acctggaacg atcgtttctt
    14221 cgatcattac cgttcagtta tttggcggga tcacctcctc aggatccagt atgatcgttc
    14281 agctattaca tgggttaggt ttaagtcaga caacaagtgt gatcttggta caggcaggta
    14341 cagactacct tgatcgtttg ttgtctatcg tcgttgtttc attagtcatc actatgttgc
    14401 cacaacgctt gatatattca aaaagaaaaa taggctgaat aaacagagct ggattagcaa
    14461 aataatgcta atctggctct gttttttcat ttattaaaaa aatcttacgc taagtaggat
    14521 aaaataattc cttgatattt ttcaatcata tccaaataat tatcgatttc aacatactca
    14581 tctacttgat gaggcaaagt aacgacacca ggcccaaaga cgacaaagtc gaaggaatgg
    14641 ttagctttcg taaattcggc tgcatccgtt gttgctgccg ctccaactaa aggtaatggc
    14701 tggctgaatt gttgctgaat gcaatgaatc aaaggagaat ctggatcagc tttgacaggt
    14761 attttgttgt aatcgatcat caattccaaa tgatagtctg tctcttgatt caattcattg
    14821 acgattgatt gcagcaatgc aatgatttta tcatttgggt attctggaat cgatcgaatg
    14881 ttcccttgga gctgcgcatg gcttgggatg ctgttgactt gattcccgcc actgatcaat
    14941 gtaacattat ggattgtttt tcctaggaca ggattctcga ttgtttcagc gaggtgattc
    15001 atttcagcat ttgctttcgt aatgaattca tttaggtgat tgattgcatt ataaccttgg
    15061 tctggcatag agctatgcgc ttcttttccg tgcgaggtga ctgtatagtt gattgatccc
    15121 atgtgcgtat acatcaaact gtaattagtt ggctctccga tgatcaaggc atctaagtca
    15181 tctacatagc ctgctttcgt gagctgttcc ccacctagtt cacccacttc ttctccgaca
    15241 gtggctaata atttgactgt tccgttaaat ggttttcctg actctttcag ttcgatcatg
    15301 gcaatcacca ttgctgccaa gccactcttc atatctgtag caccacgtcc gtacaaacgg
    15361 ttcccatgga tttctgctgc aaagggtgca taggtccaag acgattcatc accagccgcc
    15421 acgacatcca tatgcccaga taggcctaac acttttcctg attggccctt ttggtaacga
    15481 gcaatcaaat tatctctacc atctctgtaa gaaacaatct ctcctgttat gtcatgcttt
    15541 gccaaaagct tattcaaata ggctgctact tcaccttcgt ttccattcac tgatttaatt
    15601 cggatgatct cttgtaaaat cgcgattttt tcttcttttt tcatctaatc ctctcccttg
    15661 ccatttcttt ttattgtatt tgagtgtgct cgaattttca aataggagca accaaagtaa
    15721 aaatgaagaa agagagccac tttatgaaaa atatgctgct acttccctcc tattttattt
    15781 atacgagagg acttgctcat agattcctat tctactgttc tatccttaaa agctcttata
    15841 tgatgctagt tattgttagc tttccttttt caaggatgat cttttcttgt accattcttt
    15901 gtaaacatca tatacagaag ctagttgcaa aaaaaactct ctacctgctg atatt
//
LOCUS       DAZAOG010000055        15853 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000055 DAZAOG010000000
VERSION     DAZAOG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.055"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACHDLD_001458"
     CDS             complement(<1..258)
                     /locus_tag="ACHDLD_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363165.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACHDLD_001459"
     CDS             complement(274..924)
                     /locus_tag="ACHDLD_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363166.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACHDLD_001460"
     CDS             complement(935..2107)
                     /locus_tag="ACHDLD_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN3363167.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACHDLD_001461"
     CDS             complement(2126..2998)
                     /locus_tag="ACHDLD_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3363168.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACHDLD_001462"
     CDS             complement(3020..3940)
                     /locus_tag="ACHDLD_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363169.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACHDLD_001463"
     CDS             complement(3940..4209)
                     /locus_tag="ACHDLD_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363170.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACHDLD_001464"
     CDS             complement(4206..4586)
                     /locus_tag="ACHDLD_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFN3363171.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ACHDLD_001465"
     CDS             complement(5053..6516)
                     /locus_tag="ACHDLD_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3363172.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ACHDLD_001466"
     CDS             complement(6580..9597)
                     /locus_tag="ACHDLD_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3363173.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ACHDLD_001467"
     CDS             complement(9748..10587)
                     /locus_tag="ACHDLD_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFN3363174.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ACHDLD_001468"
     CDS             10732..10977
                     /locus_tag="ACHDLD_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363175.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ACHDLD_001469"
     CDS             11040..11462
                     /locus_tag="ACHDLD_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363176.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ACHDLD_001470"
     CDS             complement(11556..11873)
                     /locus_tag="ACHDLD_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363177.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ACHDLD_001471"
     CDS             complement(11900..12106)
                     /locus_tag="ACHDLD_001471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363178.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ACHDLD_001472"
     CDS             complement(12087..12269)
                     /locus_tag="ACHDLD_001472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363179.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ACHDLD_001473"
     CDS             complement(12283..12633)
                     /locus_tag="ACHDLD_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363180.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ACHDLD_001474"
     CDS             complement(12659..12961)
                     /locus_tag="ACHDLD_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363181.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ACHDLD_001475"
     CDS             complement(12964..13197)
                     /locus_tag="ACHDLD_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363182.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14101..14631)
                     /locus_tag="ACHDLD_001476"
     CDS             complement(14101..14631)
                     /locus_tag="ACHDLD_001476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363183.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14628..15743)
                     /locus_tag="ACHDLD_001477"
     CDS             complement(14628..15743)
                     /locus_tag="ACHDLD_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN3363184.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
BASE COUNT         4173 a         3346 c         2491 g         5843 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttc
//
LOCUS       DAZAOG010000056        15822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000056 DAZAOG010000000
VERSION     DAZAOG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.056"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..420)
                     /locus_tag="ACHDLD_001478"
     CDS             complement(<1..420)
                     /locus_tag="ACHDLD_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363185.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRY"
     gene            687..2387
                     /locus_tag="ACHDLD_001479"
     CDS             687..2387
                     /locus_tag="ACHDLD_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN3363186.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2357..2575
                     /locus_tag="ACHDLD_001480"
     CDS             2357..2575
                     /locus_tag="ACHDLD_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN3363187.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2595..3485
                     /locus_tag="ACHDLD_001481"
     CDS             2595..3485
                     /locus_tag="ACHDLD_001481"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN3363188.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3503..3787
                     /locus_tag="ACHDLD_001482"
     CDS             3503..3787
                     /locus_tag="ACHDLD_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363189.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4274..4888
                     /gene="gmk"
                     /locus_tag="ACHDLD_001483"
     CDS             4274..4888
                     /gene="gmk"
                     /locus_tag="ACHDLD_001483"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN3363190.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4893..5198
                     /gene="rpoZ"
                     /locus_tag="ACHDLD_001484"
     CDS             4893..5198
                     /gene="rpoZ"
                     /locus_tag="ACHDLD_001484"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3363191.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5451..7859
                     /gene="priA"
                     /locus_tag="ACHDLD_001485"
     CDS             5451..7859
                     /gene="priA"
                     /locus_tag="ACHDLD_001485"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN3363192.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7874..8365
                     /gene="def"
                     /locus_tag="ACHDLD_001486"
     CDS             7874..8365
                     /gene="def"
                     /locus_tag="ACHDLD_001486"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN3363193.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8358..9296
                     /gene="fmt"
                     /locus_tag="ACHDLD_001487"
     CDS             8358..9296
                     /gene="fmt"
                     /locus_tag="ACHDLD_001487"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN3363194.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9296..10654
                     /gene="rsmB"
                     /locus_tag="ACHDLD_001488"
     CDS             9296..10654
                     /gene="rsmB"
                     /locus_tag="ACHDLD_001488"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN3363195.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10667..11407
                     /locus_tag="ACHDLD_001489"
     CDS             10667..11407
                     /locus_tag="ACHDLD_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN3363196.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11404..13473
                     /gene="pknB"
                     /locus_tag="ACHDLD_001490"
     CDS             11404..13473
                     /gene="pknB"
                     /locus_tag="ACHDLD_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN3363197.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13638..14537
                     /gene="rsgA"
                     /locus_tag="ACHDLD_001491"
     CDS             13638..14537
                     /gene="rsgA"
                     /locus_tag="ACHDLD_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN3363198.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14550..15200
                     /gene="rpe"
                     /locus_tag="ACHDLD_001492"
     CDS             14550..15200
                     /gene="rpe"
                     /locus_tag="ACHDLD_001492"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN3363199.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15201..>15822
                     /locus_tag="ACHDLD_001493"
     CDS             15201..>15822
                     /locus_tag="ACHDLD_001493"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN3363200.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAV"
BASE COUNT         5341 a         2874 c         3434 g         4173 t
ORIGIN      
        1 atagcgataa taaatccgat tcagctgatc attaagatcg aagtatacaa aattattctg
       61 tagtttataa aaaaacggcg aatgaagatg tgttttcata tgaccaaaat ataataattc
      121 tgaaatttct aaaggtgtca attctttcag catcgttaaa tcagtaaagt cgatccactt
      181 caattcatcg aatccacgcc tgttacgaga agaggaaaag aaacgctgaa tattttctgt
      241 accacggatt actttcagcc ctgtatggga ttcatattct ccagcagaag cagatggatc
      301 aagcaagagc aagttttgag gaaaatgcac gatactgttt gcaaagtcag tagcagtcag
      361 cccttttgtt aaaacagcat tacttgtggt atcaatatgt acataaactg actcagtcat
      421 catgttcctc ctcttatttc ctcttaatct cttttatttt acaacatttg cattaaaaaa
      481 gcttgtttct tttattttaa aattccatta caaaatcacg ttttcattga aaacactctc
      541 attttcaatt aatttttgtg atttttgtat ttttttattt aaaggaggtt ttatgaattg
      601 ttttcttatg gaatctacaa agtcgaatac gagactcatg acctttttat gacgtactgg
      661 tatacttgaa gaaaaaggag agcgcgatgg agataaaaaa agtttacttt tcacagaaaa
      721 tgtcgctgta ttcgcctatc cagcaagcaa aacaacccct tccatttttt caaagtcatc
      781 aagaaagtag agacgctttt tggcgttctg caaaaaagca atgtgctttt aaagcagtat
      841 cgaaaaaata taccgtctat tttactattg ctaaaagtag aggagatgtt atttttaata
      901 atctgttgat tgaaggtgaa acttttgaat ggaaaaaatt ttttcggtat atggaagctt
      961 gcgctcgttt ttttattaaa gaaaactgtt gttttttatt tcaatctcct ctatcagaag
     1021 aatggttgcg tatttttcat aaaaacggct atcaaggaac cacacaatta aataaaaagc
     1081 tggtctatca tacagcactt gttttaggtg gaggaggagc gcacggtgct tatcagattg
     1141 gtgtgtggca ggcgctgaag gaacatgata tcaactttga gattatcaca ggtacctcgg
     1201 taggtgcgtt gaacggcgca ttgattttac aaggagatat gaaaaaagct gtgaatttat
     1261 ggaaaaagct gtcgacaaga caagtgcttg cgttaccaga aatggctgca gttgaagacc
     1321 ttcgtgaacg cttctatcga gaaagacgcc aaatgacgaa aacagcacta attgaaggcg
     1381 gggtttcttc tgctccattg gaaaagatgg tcaaagacaa tttggatttg tccttactca
     1441 aacataatcc caaaatacgt ttatatacgg tgtctactaa actgccagaa ctagcggaag
     1501 tagtgactga tatccaacag acaaaaacag aagaaatacc agattggata ctcgcctctg
     1561 cttcatttta tcccgctatg gcttatcgaa agatcggaaa aaataaatat gcagacggtg
     1621 ggtatcgcaa caaaatacct attgatattg cgattaacaa aggagcaaca gaggcatttg
     1681 tcgtagatgt gcaagggcca ggcccagcga aaaaaattcg tatgccagac atttttatcc
     1741 attggaaatg tcaaacattg tggacactag gcagctttct gctttttgat agccaaagga
     1801 accaattaaa tttacaatta ggctatctgg aaatgaaaaa acgattaggc tattactatg
     1861 ggaactggta tacatttcat tctgttaaac ctgcaagaac atactggcga ggatttttgt
     1921 cttacttaac aaaagaaatt cagttagaat tatctttttt caaaagcata aaattttggc
     1981 aaaaactgcg taatttatat aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc
     2041 tagaactttt agcaaaaaaa cattttttac tgcccaatga aatctatcaa gtagacatga
     2101 tgattcaaat gatctgccaa caagatataa aaagtatgcc ggatgattta ttggcacaaa
     2161 ttggtcaatt aagtactgaa gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa
     2221 atgagcgcac gaaaactgtt tattttgatt atctgctaca aggaaaacgc aaggatcgtt
     2281 tgcagcatga attgcttaag caaccagttg acacattact gatcctatat ttatattatc
     2341 tcaaggagga acaaccatgg cacaagaatt ttcatatgaa atcgtagaag aaatcgctat
     2401 attgtcagaa aacaacaagg ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg
     2461 cccgccaaaa ttcgatcttc gagattgggc gccagaccat gaaaaaatgg ggaaaggcct
     2521 gacattgacg aacgaagagt ttgaacaact gcaaaaagca atagaaaata tgtaatgagg
     2581 aaggaccctg tcgaatgaaa agtatgactg gttttggcaa agccatccgc gaaacaaaag
     2641 cgtatcagtt agatattgaa ataaaaagtg tcaaccagcg ttttctagat gttcaaatac
     2701 gaagtcctaa aatcctgaac tttgttgaaa acgattttcg tcagcttatt aaacagcagt
     2761 taagccgcgg tcgaatcgaa gtatttgtga atctagctta tattggcaaa aacgaaaaac
     2821 agatcgtcat caactgggaa ttgattgatt cacttatcac tcaattgaca gacggaattc
     2881 aacaacgata cggtagtcaa aatcaagtgt caattagtcg gatggcggaa cgcttcgtgc
     2941 tggacgaaaa cttcgtaacg gtagaagaaa aaccggaaga gagcatggaa gaattggcac
     3001 agttagctct agatacattg aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag
     3061 aaggtcaagc tttagcttta gtgttgaaaa agaatactga agaattcaaa gaagtactaa
     3121 aaaaactcga tagttttgta gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa
     3181 agctggagga atttcttgga gcgaaagtcg atgagcaacg gttgttgacg gagctagcca
     3241 tcttgttgga acgcggagat attcacgagg aactggatcg tctggcgatc catgtacaaa
     3301 aactagatga acttatcgaa gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc
     3361 aagaaatgaa tcgggaaatc aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa
     3421 atcaagtcgt ccagtctaag acgatcttag aaaaaatcag agaacaagta caaaatattg
     3481 aatgacgaaa gaggtagaga taatgaaata ttatgacgta acgttccacg aattaagtgg
     3541 aaaaactgtg gttaaacggg atatcccttc tgaaaaaaac ggctttgacg tatggaaaga
     3601 tgcctgtgca gactataatg agaacgaact gtttattttg atcaacgacg gtgcttacgt
     3661 gacgatgaat cggaaattta ttgttcgaat tgacacagaa gaggtagaag atccgacaga
     3721 aaaagcacgt tctcgaaaag atgagatcat gggtgtagtg aatactttgt ccaatatggg
     3781 cttttagcaa ggatgctgaa caaagaaata caataaataa acagaattgg atttcttcgc
     3841 ttagcttctt tgctctagga atacatttaa aggttgtgac aaaattcgtg tcacaatctt
     3901 tttttatttt gaataagtga tgttcaaaaa ccattttctc ggtattaagc caaatttatg
     3961 caaaaatgtg agaaactatt ttcacaaaat ttttcttcat actcgaaact aatcggcttc
     4021 ttcacgacct cttatacacg gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa
     4081 acgaaaaaca tgagaagctg tttatgtttg gctgttctta ttacttgatg caggacagct
     4141 tttttacgac ctcttggtac agggtagctt ttttaccaat ttctagctat aattaaagat
     4201 aaccttgaaa gttgcagaaa aaaagtgcaa aataaacgat aagtacaaaa agactagttg
     4261 aaaggacgtc gtcatgtcag agcgtggatt attaatcgtg ttgtctggtc catccggtgt
     4321 aggaaaaggc acagtacgaa aagcaatttt tgagagtgag gataatgatt ttcagtattc
     4381 gatctcaatg actacccgta aaatgcgtga aggggaggtc gaaggcgttg attatttctt
     4441 ccgaagcaaa gaagagtttg aatcaatgat cgaagctgga gagatgctgg aatacgcaga
     4501 atatgtcggg aattattatg gaactccttt gtcatatgtc aacaagacat tggatgaagg
     4561 aaaagatgtt ttcttggaga ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc
     4621 cgatggtgta ttcatctttt tgactccgcc agatttagcg gaattaaaat cacggatcgt
     4681 aggccgagga acagatgcag atgaagtaat cgaaaaacga atgaaagttg caaaagaaga
     4741 aatcgaaatg atggctcttt acgactatgc tgttgtaaat gatgaagtgc cgctagcagt
     4801 tgaacgaatc aaaaacatta ttgccagtga acattttcga gtagatcgcg taatcggtaa
     4861 atatattaaa atgttaaagg agatgtagtc ttatgatgtt aaaaccttca attgactcac
     4921 tattagatcg tgtaaattca aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg
     4981 aattggatgc aagtgcacag cctactctag attcttttga ttcagtaaaa agcgtcggtc
     5041 aagctttaga agaaatcgat gcaggaaatg tagtcaatga tccacatcct gaattaaaac
     5101 gtgaacgttt gaagatggaa gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg
     5161 aattggaatc acgtatccgt gaagagcaaa aaatgtaata aagaaaaata gagcgaacat
     5221 ctaaaaagaa aagcaaaccc agctgtttcc ttttcaaaca ttcgccactt attgtctggt
     5281 agaaaaggct gtgtctgaac tttttgacac agttcttttt tttcttatct gctgttaatt
     5341 ttttgttagg atttggcaga agataagaca aatccatcga tctctagtaa aataggcaag
     5401 gaactttttg tcgcttgata agaaaagaca aaaaaaggag gaaacgaacc atgacattaa
     5461 cagctgaagt cattgtggac gtacctacta tgcagaccga tcagccattc acttatttgg
     5521 ttccctctga aatggaaaca gctttgcaag tcgggatgcg agtagaagtc ccttttggta
     5581 atggcaatcg tcatgtccaa ggatttgtta tggctatcca aaaatcagaa gaatcagcta
     5641 atccatcttt gaaggcagtt attcgtttgc tagaccttgc tccggtggtc aatgaagaat
     5701 tactttcatt agctgattat atgaaaaaaa ttacttacgc attcaaaatc acttgtctac
     5761 aaacgatgct tccgagtgtg atgaaagcag aatatgataa attgatctat cctttggcag
     5821 acacaccaga agttcaggta ttgtttgatc agcgagaagt gatttcctgg aaggaagcag
     5881 aagaagaagg cagcctttct caattgattc gctggcgcca agagcaatta gtagatatca
     5941 aatatgaagt ccatacacga aataaagtaa aaacgattcg tttggtccgt tctttgttaa
     6001 ctgaaaagca aatcgaagaa gaatgggcaa aattacggca aaatgccaag aaacaaaaag
     6061 aacttctttt gtgtttatct gaactgagcc aagaagaacc aattgcttat ttcaaaaata
     6121 aaggaatcag tactgctgtc ttgaatcaag gaaaagaaaa gggctggctg gagtttatag
     6181 aatcagaacg ttatcgcgac ccttataaag accgagtatt tgaccaaacg acggcattgg
     6241 aactgaatgc agaacaaaaa aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa
     6301 aagatcaggt ctttttgctg gagggaatca ctggcagcgg gaaaacagaa gtatatcttc
     6361 aggcaatcgc tgatgttttg tcagaaaata aaacggctat tatgttagtg cctgaaatcg
     6421 cattgactcc gcaaatggtc gagcgattca aaggacgctt cggagaatca gttgcggtct
     6481 tacatagcgg actttcccaa ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag
     6541 aagcgcaagt agtagtaggc gcacgatctg ctatttttgc cccgctgaaa aatatcgggc
     6601 tgatcattat cgatgaagaa catgaatcaa gttacaaaca agatgagaca ccgcgttatc
     6661 atgcgagaga tctagcaatt tggcgcagta aatatcatca ttgcccaata gttctaggaa
     6721 gcgcgacgcc ctctttagaa tcaagagcac gagctcaaaa aggcgtttat cagttattgc
     6781 agttaaacca ccgagccaaa gcagctgcac agcttccagc tattgagatc gtagatatgc
     6841 gtgaagagtt tcagaatcat cggacctcta ctttttctgc taatttgcag gaaaaaatac
     6901 aaaatcgatt agacaaaaaa gaacaaactg tcctactgct caatcgtcga ggatattcgt
     6961 cttttgtgat gtgtagggac tgtggatttg ttcttccatg tcctaattgc gatatctctc
     7021 ttacactgca tatggataca cgttcaatgc gctgccatta ttgcggacat gaagaaccta
     7081 ttcccaatcg ctgtcctaat tgtggcggca ataaaatccg ctactatgga acaggtactc
     7141 aaaaagtaga agaggaactc cgagaacttt atccgcaagc taggattttg cgtatggatg
     7201 tagataccac tcgaagaaaa ggggcacacg aacagatttt acaaaaattc ggtgcaaaag
     7261 aagcagatat attattaggg acacaaatga ttgctaaagg tttggacttt ccagaagtga
     7321 cattagttgg ggtgttgaat gctgatacct ccctgaattt accggatttt cgttctagtg
     7381 agcatacatt ccaattgctt acccaagttt caggacgagc agggcgagcg gaaaaagcag
     7441 gcgaagtcgt gatccaaaca ttcaatcctc aacattacgc catagaattg gctaaaaaac
     7501 aaaattatga gcagttttat cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc
     7561 cttattactt tactgtgaaa ataaccgcca gccatcctga agaacaagta gcggcaaaga
     7621 aaatctttca gattgccaat cagctaaaag aagtgttaag ccctcaaagc ttgctattag
     7681 ggccaacgcc cagcatgatt ttgagagtga aaaaccgata ttattatcaa ctaatcatca
     7741 aatataaaca tgaaccgaat cttcctcatg ttttggatga aattttgaat ggaagccaga
     7801 aagaacagcg acaaggtctt tttgtggcaa ttgacaacga accattaaac tttatttaag
     7861 aaagggccaa attatgcgct atcctatatt gatccaccct aatgataaat taaaaagaac
     7921 tgctcagcca atcgatgtaa tcacggacga gacaatcgct ttattagaca atttatatga
     7981 gacaatgatt gcgaatgacg gaatagggat cgctgctcct caagtaggac aaaacaagag
     8041 aattgctgtg atcgaagtag atgaaggcga gaaatttgaa ctgattaacc cagaaatcat
     8101 cgaagcaaaa ggtgaaagcc ttgacgtaga aggttgtctc agtattccgc atgtctatgg
     8161 aacggtcaag cgagcagatg aagtgaccgt gcgttattat gatcgagacg gagaagagat
     8221 agaagtgacg gcttttggct atttagcccg tgccttccag catgaaatcg atcacttaga
     8281 cggtatcttg ttcattgaga aaatgataca gcagatcccg gaagaagaac ttgaagaata
     8341 tatggaggaa catcttgatg actaagctag tatttatggg cacacctgct ttttctgtgc
     8401 cgattcttga agggctgata gaatcaggtt acgagatcca ggcagtggta acacagcccg
     8461 accgtccagt tgggcggaaa cgagtaatta cgccgacacc ggtaaaaaaa gcggctctga
     8521 agcacggtat ccgtgtgctg cagccagaga aaatctccgg ttcgccagaa atggaagaaa
     8581 tcattgaatt ggtgcctgat gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa
     8641 agttattgca agtaccaaaa ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat
     8701 atcggggagg tgcgcctgtt cattatgcca tcatgaatgg ggagaaagaa actggtgtaa
     8761 cgatcatgga aatgatcaaa aaaatggatg caggcggaat ctatgcgcaa gaaagcatac
     8821 caatcactaa acaagatgat gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa
     8881 agctattgct agaaacattg cccaatattt tggatggaca aaaaccagta cctcaagacg
     8941 agtcagaagc aactttttcg ccaaacatca cacgggaaca agaagcaatc gattggcata
     9001 aaactgcaga agaaattgac aaccaagtac gcggaatgcg tccttggcca attgcattca
     9061 cgacctatga gggcatacgc tggaaattat tatcggttga agtattggca gaaactacag
     9121 acaaagcacc tggtacgatc atccggaaag acaaaaaaaa tctatggatt gcttgcggta
     9181 aacagaccgt cttagccatc aatgaattac agccagctgg taaaggaaaa caggccgtac
     9241 atgatttcct taacggaagc ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa
     9301 aaaaatccct aaaaaaatga aaaaatcagt acgatatatg gcattactga cattagagcg
     9361 agtgaacaag ggtggcgcct actcgaatct tttgttgaat gagatgatca aacaaagcga
     9421 gctaaatcaa aaagatgtgg gtttatttac agaattgatc tatggtacga ttagccatca
     9481 gcgactattg gaattttacg ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg
     9541 ggtcaagatg cttctttatc tttctgtata ccaattagaa tttttagata aagtccctgc
     9601 acatgctatt ttaaacgagg cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg
     9661 gaaatttgta aatggtgtct tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg
     9721 cattgctgat cctatcgaaa gactttctgt caaaatcagc ctgcctctat ggatgacgaa
     9781 acgactggtg gatcaaatcg gatacgaaga aactgaaaag ctcggtttat cgctatatca
     9841 gcctagccat gctagtgccc gagtagatac acgccgactt tccagagatc aggccatcac
     9901 tgttttgcaa gaagaagaga tcacagctga aaaaagccaa gtcagtccat acggtatagt
     9961 agcgaaaaaa gggcatttag cttctagctc attgttccac gatggcgtca tgacgatcca
    10021 agatgaaagt tcgatgcttg tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt
    10081 agatgcctgt gcagcacccg gaggtaagac gacgcatatt gcttcttttt tagatccgtc
    10141 atgcggtgga gaagtggtcg ctttagatgt acacgaacac aaagtcaaac tcattgaaga
    10201 taatgcagca cgtctaggtg tctcttctgc tgtctacgct caaaagatgg atgctagaga
    10261 agtcagtaaa cagttcgaag cagagcagtt tgatcggatt ttagtagacg cgccttgttc
    10321 aggtcttggg ctgcttcggc gaaaaccaga tattcgttat aaaaaacagc cggatgaatt
    10381 agcgaattta ccaaaaatcc aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc
    10441 ttccggtatt ctgacttata gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt
    10501 ttctgctttc ttggaaagac accctgattt tgagaggatt gatgttttga ccaatcctat
    10561 gctacaatca tcaatcgaag agaaaatgtt gactttgtat ccgcatcagt tttacacaga
    10621 tggatttttc atttgttgtt tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata
    10681 tcaatcagat gtaggaagac gacgtaacac aaatcaagac tatgccagtg tatttaccag
    10741 ccgttcaggt ctcaagcttg ctgtattagc agacgggatg ggtggtcatc gtgccggaga
    10801 cattgccagt cagatggcgg taacgaatct aggcgaagct tgggaaaaac aagaagctgt
    10861 tgacgatgaa aaaatcgccc aatggttcat tcaatcgatt caagaagaaa atatgctgat
    10921 ttatcagcgt ggacaagaac agccggaata taacggtatg ggaacaacaa tcgtagcggc
    10981 cgctctgtca gatgactggt tcacgattgc caatgttggg gatagcagag cttatttgat
    11041 tcgtgaagat aaaatcatgc aattgacgga ggatcattct ttagtcaatg aactagtcaa
    11101 atcaggagaa atcagcgaag aaatggcagt gaaccatcca aggaaaaata ttttaacacg
    11161 ttctgtcggc atgcctggaa cagtcgaagt agatgtttct actcatcttt ggcaactaaa
    11221 agatcgactg ctgttatgtt cagatggctt gacgaacatg ctgtctgaag aagaaatagg
    11281 aacgatcatc aatcaagaag gcccattgtc tcataaagta actgagctga ttgatcgtgc
    11341 caatgaagca ggcggagcgg ataatatcac cgttcttctt atcgaatata aggaggacag
    11401 tgtatgatcg agttgggcaa aaaattaaat ggccgttatc aaatcactgg aaatatcggt
    11461 agcggtggta tggcaaatgt atttttggcg cacgatttaa tcttggatcg tgatgtagca
    11521 gttaaagtct tacgatttga ctttcaaaat gaccagactg cgattcgacg atttcagaga
    11581 gaagctttag cagcaacaga actcgttcat ccaaacattg ttagtgttta cgatgttggt
    11641 gaagaagaca acatgcagta tctggtgatg gaatacgtca aaggaatgga cttgaaacga
    11701 tatatccaaa cacattatcc tgttccttat gaaacagcag taaatattat gcagcagatc
    11761 ctttctgcta tttctttagc tcataaccat caaatcatcc accgagattt gaaaccccaa
    11821 aacgtactga tcgataatga aggtgttgta aaaatcacgg atttcgggat tgctatcgct
    11881 ttatctgaaa catcgatcac gcagacgaat acgatgctag gatctgttca ttatctgtcc
    11941 cctgagcagg ccagaggaag tatggcaacg aagcagtcag atatttatgc tttagggatc
    12001 atcctttatg agatgttgac gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt
    12061 gctttaaaac attttcaaga tgatcttcca tctataaaag cacttgatcc aaatgtgcca
    12121 caagcactgg aaaacgtgat tttacgggca acggctaaag aacctgcaga ccgttataag
    12181 tcagccgagg aaatgtctga tgatctttct acagtattat caccagctcg tgcaaacgaa
    12241 gaaaaatggc agcctcatgt gatggataat gaaacaaaag ttatcacacc ccttacggaa
    12301 gatgcaccaa tgcctgattc attcaaatct atgcctctgc caaaagacaa gccagaaaac
    12361 gaaaccttac cagaggagga agaagttctg cctgatccta aaaagaaagg acagaaaaaa
    12421 tggtggctga tcctattagc tattcttgct gttttaggaa tcggaacagc tgtattcttt
    12481 gcttctggcg ggcgaggaga agttgttgtt cctgatgttt ctgatctttc tgaagcttct
    12541 gctagagaaa cactcgcaaa agcgaaatta gaagtagcaa caaaaacaga agaaattgca
    12601 gatgacaaaa tcgaagaagg taatgtagtg aagacagacc cggcagcggg aacgacagtc
    12661 aagcaaaaac gcgaagtcac tctgtatatt agtagcggca cgaaaaaaat caaattagat
    12721 gattacagtg gtaaaagtta tgaagatgcc gaagcaagtt tgaaagaatt agggttttct
    12781 gctgacttga ttcaaaaaga agaggaattc agttcagagg tagaaagcgg aatgatcatt
    12841 tcgcaatctc catcagaaga tacagaagtc gatcctaaaa cagacacgat tactttcaca
    12901 gtcagcaaag gaccagaaac agtgactttg agtagtcatg taggagaaaa ctacgaaact
    12961 gttcgtaatt ggcttgttac acaaggattc aatatcaata atattagtgc tacttatgac
    13021 tatagcgaca ctgtaccatc aggttcgatc atcagtcagg atccagcctc gggtgaagtt
    13081 gtagcagaag aaacttacat caattttgtc gtaagccaag gaacagagcc taaattgaat
    13141 gatatctccg gctatacaaa aagcgaagca caaagttatt tagctagtgt cggtgcagaa
    13201 tatatcgggc atgagacttc cgaatattca aacacggtgg ataaagataa agtgattcgt
    13261 acagtaccag gggcaggaac gacaattacc aaaggaatgg ttgtgaacgt aatttactct
    13321 aaaggaccag atccagcgtc ttctagttct gaaaaagact cttcagacag ttctagcagc
    13381 tcaagtagct ccagtagcac aagcaactct tcaaaagaag aatcttccag ctccacaagt
    13441 tcatcagcta gcacctcaga aagttcaaaa taagtgctga ataaaatata tcagaaggga
    13501 atgcgacatt gatttgttgc attctctttt ttgtgtttct atctcttttg atcaattttc
    13561 aagccatata ctttgagaag tttgcatgga ataacaccta ctttcctctt cgtatggtaa
    13621 aataaacaaa aacggaggtg gcacttatca aaggacaaat cagaaaagca atcagcggtt
    13681 tttactatgt atacgcaaac ggagagacct atcaaacaag aggaagagga aatttccgga
    13741 atcggaaaat cacgccgctt gtcggagatc aggtgatctt tgagagtgaa aatcaaacag
    13801 acggctatct tctggaaatc ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa
    13861 atgttgatct tggaatcatt gtcacaagta tcgtagagcc tgcattttct tataatctac
    13921 ttgaccgctt tctagttacc ttggaatacg agtcgatcga accgattatt tttttgacaa
    13981 agaccgactt gtcagacgac ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa
    14041 taggctatcc ggtgattgta cccaatcata cgggtgatac gacagaactg atccgttact
    14101 tcccagaacg gctatcggtc tttatgggtc agtctggagc aggaaaatcg acgctactga
    14161 atcaaatatc accggaactg aatttagcaa caggagagat ttctgatgca cttggaagag
    14221 gacggcatac aacacgtcat gtggaactga ttcctatcta tggcgggtta gtagcagata
    14281 caccaggatt tagctcgatt gactttttga caattgaagc aggagaactg ccgagacaat
    14341 ttcctgaatt tgtagaagct tctgtccatt gcaaatttag agagtgcatg catgctaaag
    14401 aacctggctg cgaagtaaaa catagagtag aaacaggtgg tatcgcacag acacgttacg
    14461 acaattattt gcaattctta caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag
    14521 aaggtaaacg aaaataggag gaaacaagaa tgaagatagc accatccatt ttgagtgcag
    14581 attttgccaa tttacaaaga gacattgagt tagtggaaaa aggaggcgca gattacatcc
    14641 acgtagatgt aatggatggc cgatttgtcc caaatatcac attagggcca aatattgttc
    14701 aggcaatccg tcctgttact aaattaccgt tagacgtcca tctgatgatc gttgatccag
    14761 aaaactacat tccagcattt gcaaaagctg gagcagatat cattactgtc cacgtcgaag
    14821 caactcctca tattcatcgg gcacttcaaa tgacaaaaag cttaggagtt aaagctggag
    14881 tagttatcaa tccaggaaca ccagtctcca tgatcaaaca tgttttgcct ttggctgatc
    14941 aagtactagt catgactgtc aatcctgggt tcggcggaca aagctttatt gaagaaacag
    15001 tagaaaaaat tacggaactc gctgatctac gtagagagaa caattggact tatgcaatcg
    15061 aagtagatgg cggaatcgtt ccagaaacag caaaaatctg taaagatgca ggggcggatg
    15121 tcttcgttgc aggatcatat atctatggag ccgaaaatcc agtcggacaa atcgaacgat
    15181 taaaagaggc attagactaa atgaaaattt tattagctgc tggaggtaat ccgcaaaact
    15241 ggccgcagtc tctgctgtcc aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt
    15301 acctgcttca agcaggctac ccagtagatc tggcagtggg tgattttgat tcattgtcga
    15361 acgaagagca gaaggaagta tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa
    15421 aagacgatac agatacacag ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg
    15481 aagtcacttt gattggagct acgggcggaa gattggatca tcttttagcc aatctctggt
    15541 taggtatgga acctcgcttc aaaccctttg cccatcagct tattatcaga gattgtcaaa
    15601 attatttgtc ttattatttg cctggagagc atatagtaag acagatacca gatatgaagt
    15661 atttagctta ttgctgcttg actcccgtct cgaatcttac attaaaaggc agcaagtatg
    15721 tattggaaaa taaagaagta gaatatccga catcttatgc aagtaacgaa tttctttcag
    15781 ataccgcaga attttctttt tcagatggta tgattgcagt ta
//
LOCUS       DAZAOG010000057        15793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000057 DAZAOG010000000
VERSION     DAZAOG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.057"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..773
                     /locus_tag="ACHDLD_001494"
     CDS             21..773
                     /locus_tag="ACHDLD_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3363201.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            770..1684
                     /gene="pfkB"
                     /locus_tag="ACHDLD_001495"
     CDS             770..1684
                     /gene="pfkB"
                     /locus_tag="ACHDLD_001495"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN3363202.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1702..3633
                     /locus_tag="ACHDLD_001496"
     CDS             1702..3633
                     /locus_tag="ACHDLD_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN3363203.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3744..5303)
                     /locus_tag="ACHDLD_001497"
     CDS             complement(3744..5303)
                     /locus_tag="ACHDLD_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN3363204.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5458..7692
                     /gene="pcrA"
                     /locus_tag="ACHDLD_001498"
     CDS             5458..7692
                     /gene="pcrA"
                     /locus_tag="ACHDLD_001498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN3363205.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7709..9745
                     /gene="ligA"
                     /locus_tag="ACHDLD_001499"
     CDS             7709..9745
                     /gene="ligA"
                     /locus_tag="ACHDLD_001499"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN3363206.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQGQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9851..10156
                     /gene="gatC"
                     /locus_tag="ACHDLD_001500"
     CDS             9851..10156
                     /gene="gatC"
                     /locus_tag="ACHDLD_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN3363207.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10156..11628
                     /gene="gatA"
                     /locus_tag="ACHDLD_001501"
     CDS             10156..11628
                     /gene="gatA"
                     /locus_tag="ACHDLD_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN3363208.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11625..13055
                     /gene="gatB"
                     /locus_tag="ACHDLD_001502"
     CDS             11625..13055
                     /gene="gatB"
                     /locus_tag="ACHDLD_001502"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN3363209.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13065..14120
                     /locus_tag="ACHDLD_001503"
     CDS             13065..14120
                     /locus_tag="ACHDLD_001503"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN3363210.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14231..15604
                     /gene="rlmD"
                     /locus_tag="ACHDLD_001504"
     CDS             14231..15604
                     /gene="rlmD"
                     /locus_tag="ACHDLD_001504"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN3363211.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15607..15735
                     /locus_tag="ACHDLD_001505"
     CDS             15607..15735
                     /locus_tag="ACHDLD_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN3363212.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5369 a         2899 c         3474 g         4051 t
ORIGIN      
        1 gtattgaggt gatcgtgaaa atgcttacag aggaacgcag acaaaaaatc ctagacttgc
       61 ttgaacagca gaaaatcgtg aaatcacaag atcttgtaaa ccttttgaat gcttctgaat
      121 caacgataag acgtgattta caagagctgg agaatagtgg gtttctagaa agagttcatg
      181 gcggagctaa gaaagagcaa ttattgaatt ttgagcagaa tatgagtgaa aaatcactca
      241 aaaacgttca cgaaaaacag caaatcgcca aatcggcagc tgaaaccatt aatgacgaag
      301 atgtcattta tttagacgct ggctctacta cgttagaaat gattcctttt ctgcaaggaa
      361 aacagatcac agtagtgaca aattccgttc aacatgcggc aaatttagtt gatttggagc
      421 ttgctaccat tattttaggt ggaacgataa agctggcaac taatgctgtt ttaggatcta
      481 atgcgataga acaactaaaa aattttcatt ttaataaagc atttatggga atgaacggtg
      541 cccatcttga acaaggattc acgactccgg atccagaaga ggcagctgtt aaacgtttag
      601 ccatgaaaaa ttccgaagag acatttgtat tgattgatca taccaaattc aaccagttgt
      661 ccttcacctc tgttgcacct ttggaagcag ccacgatcat aaccgagcgt tgtccattag
      721 aattcgttaa tgaattccgt gaaaagacaa caattaagga ggcaatacaa tgatttacac
      781 agtaacactt aatccatcaa ttgattatat cgtacgcgta gatgggttaa aactaggcga
      841 tttgaaccga atgaaggaag acttcaagtt acctggagga aaagggatca atgtttcccg
      901 tattttgaaa cggatcgatt ctcaatctac agcactaggg tttttaggag ggttcactgg
      961 cgaatttatt tcgaagtggc ttgaaaaaga acaaatccag tctttgttca caaccgtaaa
     1021 agaagataca agaatcaata tcaaactaaa atcagacact gaaactgaga tcaatggctt
     1081 aggcccatct atttcagatg aagaaatcga tgagttgaag caagcattag gaacagtagg
     1141 acaaggagat atcgtcgtat tgtcaggaag cgcgccagct agcctgcgaa aaggatttta
     1201 tcaagaactg attgatatca ttcgagaaaa aggtgcagat ttcgtgattg atacaacagg
     1261 tgatgaccta aaaaatgcac ttgttaagaa accgttgttg atcaaaccaa acaatcacga
     1321 attagctgag ctttacaaca caaccttcca atctgtagaa gaaatcattc cgtatgggaa
     1381 aaaattacta gctgaagggg cacagaatgt gctgatctca atggctggag acggtgcttt
     1441 gctcttcacg gaacaaggaa cttataaaac gaatgtatta gttcgcccat tgaaaaattc
     1501 agtaggtgca ggggattcaa tgattgctgg ctttattggg gcttacagcc gtacgaaaga
     1561 tccaatcgaa gcctttaaat ggggagttgc ttgcggaagc gcaacagctt tttctgatga
     1621 cttggcagct cgcgaattga ttgatgagtt gctgccagaa gttgaaatca caaaaattga
     1681 ttagtttcaa ggaggataat catgaatatc aaggatttaa tggtcaaaga agcgatgatc
     1741 atggatttgc aagcgactga taaaaaaggc gcaatcgatg aaatggtcca aaaaatgttt
     1801 gatgccggcc ggatttccga tatcgaaaca tataaagaag gaattctagc ccgcgaagca
     1861 caaacttcta caggtcttgg tgatggaatc gccatgcctc acgcgaaaaa cagtgcagtt
     1921 aaagaagcaa cagttttatt cgcaaaaagc caaaaaggag tagattatga agccttagac
     1981 ggacagccaa cttatctgtt tttcatgatc gcagctccag aaggcgcaaa cgacacacat
     2041 ttgcaagcat tagcagcact atctcgattg ctgattgatg cagatttcgt aggaaaatta
     2101 aaagaagcaa atacaccaga agaagtccaa gacttgttcc aaacagctga agagcaaaaa
     2161 gaagcagaag aaaaagcaga acaagaaaca caagctgcag aagcttctgc tcatccagaa
     2221 agaaaattcg ttgttgccgt tacagcttgt ccaacaggga tcgctcacac ttacatggca
     2281 gaagatgctt tgaagaaaaa agcgaaagaa atgggtgtgg agatcaaagt cgaaacgaac
     2341 ggttcagaag gaatcaaaaa ccgattgact gctgaagaca tcgcccgtgc agaaggtgta
     2401 atcgttgcag cagacaaaaa agtagagatg aatcgctttg acggaaaaga actgatcaat
     2461 cgtcctgtca gtgatggtat ccgtaaacca gaagagttga tcaatctagc ccttagtggt
     2521 actgcaccag tattccatgg tgatggaaaa gaaagcagtt cagaagaagg aaatgcggat
     2581 ggtactatcg gacaaagaat ttacaaagat ttgatgaacg gcgtatcaca catgttacct
     2641 ttcgttattg gtggaggaat cgctattgcg ctttctttca tgatcgacca atttattggc
     2701 gtgccacatg atcaattatc aaatctaggg aattacaatc aagctgctag ctggttcaac
     2761 caaatcggac aagctgcatt tggtttcatg ctgccggtat tagcaggatt tattgcttca
     2821 agtatcggtg accgccctgg tttgatcgtt ggatttgctg ctggtgcatt agctaataca
     2881 ggcggtgcag gattcttagg tgcactgatt ggtggattct tagctggtta tgtcatcgta
     2941 tttttacgta aattattcaa gaacttacca aaatcattgg atggaattaa aacgatcctc
     3001 ttttatccag tgtttggctt actgattact ggtttcttga tgttgctggt caatgtccct
     3061 atgaaagcaa tcaatgatgc attgaacggt ttcttgacaa gtatgagtgg ttcgaatgct
     3121 gctttacttg gtgcattatt agctggaatg atggcggcag atttaggcgg tccaatcaac
     3181 aaagccgctt atgtgtttgg tacagcaact ttagctacga ctgttgcaac tggcggaagt
     3241 gtcgtgatgg cttctgttat ggctggagga atggtacctc cattggcaat tttcgttgct
     3301 acacgtttgt ttaagaataa attcagcaaa acagatcaag atgctggttt gacgaacatc
     3361 gtgatgggac tatcgtttgt cacggaagga tcgattccat ttgcagcagc tgacccgatt
     3421 cgtgccattc caagttttat tattggttct gctttaactg gcggattagt cggagctttc
     3481 ggaatcaaat tactggctcc tcacggcggg attttcgttg tattcttact gagtcacccg
     3541 gtcatgtatt tggtgtttat tgctatcgga gcagtcgtat ctggtgtgat ttatggttta
     3601 ttgagaaaag ctcctgcagt agcagaagca tagaaataaa aaagaagagg ctgggttttc
     3661 ccagcctctt ctttttttat agaatcatat agaatcaagt agtgtagatg aatagttgtt
     3721 gtaaaatagt ttttccatgt tcgttaaaat gattgcttca aatttgtgat agaagattta
     3781 gcctcgattt cctgcttgat tcttggcaag tttgcttcat cagtcaagat aaccaaaata
     3841 tctcctacat tcatgatcgt atcaccgtga gtcaagattt cctgatttcc tctgcgtata
     3901 gagattagca gcatattttt aggccaattg aagtctcgta ccattaatcc gtctaaagaa
     3961 ctctctgccg ttactggaaa atcaaaagtt gtccttgcac cttttagtga ggcggtctga
     4021 gtatttccaa gtagacgttc caataatgat tcgtaaattg gattaccgcc gagaaggtca
     4081 ttgacgatat aagcaacaag agcagccaat cctaaaggca tcagatgatt aagagagcca
     4141 accatttctg tcactaaaat aattgctgtc agcggtgctt ttccaatcgc tgtgaaataa
     4201 ccagccatgg caataacgac aaaatccttg atgtagattg gatccatccc gaaatcaacc
     4261 ataatgtttc cataaaagct accgattaaa gcccccaatg tcaatatcgg aaggaaaatc
     4321 ccccctggaa gacccccacc gtaagaaatc atggaaaaaa cgaatcgaag aacaaacaaa
     4381 ccgataatca taatgattgg caaggaagca tgtccaaaag ctaaaacgat ttgattgcct
     4441 ccgcctagag tactaggcca taaataacca attggcaaaa tcagcaagaa aggaacgaac
     4501 ccgtgaaaat aagctggtaa aggcaatcgg ttgtaaaatt ttggcaaagc cagcaagaca
     4561 ttctgataaa taaaacctaa gatgcctaga agtactccta agatcactaa ggaaccataa
     4621 tatttcaacg gcaaagaagg aaggtcacct agatataaaa caggtctcaa gccgaaaaag
     4681 taaagagaga cgaagttagc agtgattgct gaagcaaatg atgtcagcca aacaagtgga
     4741 gaaaaggagt gatggatttc ttctaaaaca aagagtaatc ctgcgatagg agcattgaat
     4801 gctgctgcaa gtccgctact tgcaccgcta gaaattaaaa tcttttcttc tgaagatgga
     4861 ctccgaaaca attgtccagc tccttgtcca atggatgcgc caagctgtat agaaggtcct
     4921 tctctaccta gaaaaaggcc cgcaccaact gaaagtacac cgccaatgaa ctttttccat
     4981 aaaacagaga accagttcat tgataattcc ccgcgaagct gtccctctac ttgtggaatc
     5041 ccgcttccct tgatatccgg ttcatttttg accagataac ccacgaaaaa tgctgcaagg
     5101 atgcagcaaa tagctaggcc aattaagtaa atcggctgtt cccgactcat ttcatagata
     5161 cttcgaacta aatcgcttag cttttcgata gataatcgaa acaggctaac gacgattcct
     5221 gtcagcagtc caacaaatat tccttttaga ataaaatata tctgagtctt atctaatttc
     5281 ttgatttttc cttttttttc cattcaaacg cctcgctttc ttttaattta gcgcgaaatc
     5341 aacgaattga caaggaaaaa tccataaaaa gaaacaaatg attttgaact agctagttta
     5401 atgaaagcca agaaaatgct ataattaaca agattgataa aagaaaggat gttgttaatg
     5461 tcatccaaag ctgaattatt aaacgggatg aacccccgtc agaaagaagc tgttcttcac
     5521 actgacggac cattactgct aatggcaggt gcaggaagtg gaaagacgag agtgctgaca
     5581 caccgcattg cctatttgat tgaggaaaaa gaagtcaatc cttggaacat cctagcgatt
     5641 acttttacca ataaggctgc aaaagaaatg aaagaacgtg tcaatgcgat tctcgcatct
     5701 ggaggcgaag atgtctgggt atcgactttt cactcgatgt gtgtgcgtat tttaagaaga
     5761 gatgttgatt ttatcgggta taaccggaac tttacaatca tcgattcttc tgaacagctg
     5821 accttgatga aacggatact aaaagaactg aacattgatc cgaaaaaata tgatcctcga
     5881 agtatattag gaacaatctc tcaagcaaaa aacagcctgc agacccctca ggattttgca
     5941 aaaatgcaag gcagttatta cgaagagatt gcggcaaaat gttatgctgc ttaccaaaaa
     6001 gagcttcaat ataatcaatg tatggacttt gacgacctga tcatgaatac gattcgatta
     6061 tttgaagaac atcctgattc actcacgtat tatcaaaaca aattccatta tattcatgta
     6121 gatgagtacc aagatacaaa tcacgcacag tatacgctag tcaatttatt ggcaggtaga
     6181 tttaggaacc tgtgcgtcgt gggagatgca gatcaaagta tttacggctg gcgcggtgca
     6241 gatatgcaga acatcttgga ttttgaaaaa gattatccag atgctgcagt aattttgcta
     6301 gaacaaaact atcgctccac gaaaaacatc cttagcgcag caaaccaagt cattgaaaac
     6361 aatagcaatc gaaaaccaaa aaacttgtgg acagaaaaca aagaaggaaa caagatcact
     6421 tactaccgtg cagataatga acgagacgaa acccgtttta tcgtagaccg gatgcaagaa
     6481 gagattcgaa gcaatcatcg gaattacggc gactttgcca tcttgtaccg gacgaacgca
     6541 caatctcgtg taatggaaga aactctgttg aaagcaaata ttccttataa aatggttgga
     6601 gggcacaagt tctacgatcg taaagaaatc aaagatattt tagcgtattt gaatgtgctg
     6661 gtaaatcctc aagattcaat cagttttgag cgtatcgtta attcgcccaa acgaggcatc
     6721 ggaccaggat ctatcgaaaa actacgcagt tttgcttctc ttcatgaatg gccactgctt
     6781 gaagcagcgc aaaatgtgga tctggcaaat atcggtggaa aagcaggaca gcaattaggt
     6841 gcatttggag agatgatcca agaagtcact cagatgatcc catacttgac agtgacagaa
     6901 ttgacgaaag aggttttgga tcgtagcgga tatctagaag acttaaaaat ccaaaataca
     6961 ttagaagcac aagcacgcat cgaaaacttg gaggaatttt taacggtcac acaagaattc
     7021 gacaaacaat tcgaacagca aaatgaagaa gacgctgatg caccagaaga aaaactaacg
     7081 gtcttcttaa atgatctggc acttgtttcc gacattgaca atttagaaga agatgcttcc
     7141 caagtgacat tgatgacttt gcatgcagcc aaaggattag aatttccagt tgtattcctg
     7201 attggtctag aagagggggt ttttccttta tctcgagcat tgatggaaga aagcgaactg
     7261 gaggaagaac gccgtctcgc atatgtgggg atcacgcgag cagaagaagc gttgtattta
     7321 acaaatgctt tctccagaac attatatgga cggacgcaat ataaccgacc ttctcgtttt
     7381 gtagaagaaa tcgatcagga gttactggaa atcgaaggca tgcgtcctac gcctaaaaaa
     7441 acgcctgtat tcgctaaaaa aacagcatat agctataaac agccagagac agcggttgtt
     7501 ccctctaaaa gtgctactgg aggggaaaat aacagctgga aacctggaga taaagtcaaa
     7561 cacaaaaaat ggggtctagg aacagtcgtc cgagtaagcg ggacgtcgaa ggatctagaa
     7621 ctagatgtgg ctttcccaag tcaaggagta aaacgtcttt tggctgcctt tgcgccaatt
     7681 gaaaaagctt agtagtggag ggtaaatgat ggaacatgaa atgacgattg acgaagctgc
     7741 taaacgagca gaagaacttc gtacaagact gaaccaatgg tctcgcgaat actacgtaga
     7801 agataaacca acagtggaag actatgttta tgataaagaa tacgcagaat tagtagcaat
     7861 cgaggaacaa tatcctgatt tgatcacgtc tgattctccc actcaacgag taggcggaaa
     7921 agtattagaa ggttttgaaa aagtcactca tgatattccg ctttacagtt tgaacgacgt
     7981 tttcagcaaa gaagagctga tagcttttga tcaacgtgta caaaaagcag tgggacgtgt
     8041 agttgactat tgctgtgaac taaaaataga tggactttcc gtttctttgc gttatgaaga
     8101 tggaaatttc gtgagaggtg cgactcgcgg ggatggaact gtaggagaaa acatcactga
     8161 aaacctaaaa actgtccggt cggtcccaat caaattaaaa gaaccaatga acatcgaagt
     8221 aagaggcgaa tgtttcatgc caaaacggtc atttgtccaa ttgaaccaag acagagaagc
     8281 agagggaaaa gatatctttg cgaatcctcg gaacgccgca gctggttctt tgcgtcagtt
     8341 agattctaaa atcacagcga aaagaaatct ggacactttt ttatacacag tagctgattt
     8401 cggtcctatg caagcaaaaa cccagtatga tgcgctagaa gagctagaaa aaattggatt
     8461 ccacacgaat cgggaaaaac gtctatgtca ttcgattgat gaagtgtggt catacattga
     8521 agaatatcat gacaaacgtg tggatttgcc ctatgaaatc gatggaatcg tcatcaaagt
     8581 gaatgaattc tcgctacaag gtcaacttgg ttttactatc aaagcaccga gatgggcagc
     8641 agcttacaag tttccgccag aagaagttga aacgctaatc gaaaacatcg aatggacagt
     8701 cggtcgaaca ggggtagtaa cgccaacagc tatcatgaca ccagtccgag tagcaggaac
     8761 gacagtaagc cgtgccagcc tgcataacgg tgattatatt aagttgaagg atatccgctt
     8821 gaaagataca gttctgattt ataaagcggg agacatcatc ccagaagttt cgcaagtcgt
     8881 gttagataaa cgacctaaag acagcgaaga ataccaattg cctacacatt gtccagtttg
     8941 tggaagtgaa cttgtccatt tggatgaaga agtagcctta cggtgcatca atcctaaatg
     9001 ccctgctcaa atgaaagaag gtttgaacca ctttgtttca agaaatgcga tgaatatcga
     9061 tgggttgggg ccgcgcgtac tagaacagat gtatgataaa aaactagtag cagatgttgc
     9121 tgatctttac aaattgacag aagaagagtt attgacgttg gacaaaataa aagaaaaatc
     9181 agcaaataat attttgacag caatcgacaa cagcaaagat aattcggtcg agcggttgat
     9241 tttcgggtta ggaatccgac atgtcggagc gaaagcagca aaaattttag cagaacattt
     9301 cggtgatcta gaaacattga gtaaaagtga ctatgagtcg attattgcgc ttgatacgat
     9361 cggtgatatc attgccgaca gtgtagtcac ttatttcagt aacgaagaag tccatgaatt
     9421 gatgaatgaa ttaaaacaag caggcgtcaa tttcgaatac aaagggcttc gaaatgccca
     9481 attgcaagaa gtcgagtctc cttttaaaga aaaaacagta gtccttactg gtaaactaac
     9541 acgatttact cgtgaagaag caaaagagac gatcgaaaat cttggcggga aagtgactgg
     9601 tagcgtatcg aaaaaaacgg atatcgtggt agcaggagaa gatgctggca gcaagctgac
     9661 caaagcacaa gaattaggaa tcgaagtttg gacagaagat caaatggcag atgcattggc
     9721 taaaagtcgc tcagtagaag aatgaaaaat gagttttttc agacaaattt caataaacaa
     9781 tttcagacac ggcactcttg tgttaaaata gagagcaaag tttttaactc gtttagaaag
     9841 aaggggtagt atggcgatta gtgaagaaca agtgaaacac gttgccaaat tagcaaaact
     9901 tgcgttcgca gatgaagaac ttgcaagttt taccgaccaa ttaggtaaaa tcatcgatat
     9961 ggttgaaatg ctggaagaag tagatacgga aggtgttcca tttacgtcga atgtggttga
    10021 aacagtaaac gtgatgcgac aagatcgggc gcaagctgga tggaaccgcg atgaattatt
    10081 gaaaaatgtg ccggaaacag aagacggctt tatcaaagta cccgcaatca tcgataatgg
    10141 ggaggctggc gcataatgac agaattacac aatcgttctc tagaagaact gcatcatatg
    10201 cttgtatcga aagaaatcac tgtacaagat ttgacaaaaa caacatttga acgaatcaaa
    10261 gaaacagagc ctgaggtcga ggcattcatt acattgaacg aagaaaaagc attggaacag
    10321 gcaaaagctt tagatgaaaa agggattgat gaaagcaacg tccttgctgg tattccaatt
    10381 ggtatcaagg ataatatcgt aacaaaagat atcttgacaa ccgctgcatc taagatcctt
    10441 tacaattttg aaccaattta tgatgcaacg gtcatggata aaatctatca agcagatatg
    10501 atccctgttg gtaagctaaa catggatgaa tttgctatgg gtggaagtac agaaacttct
    10561 tatttcaaga aaacaaaaaa tgcttgggac ccaacaaaag tacctggtgg ctcttctggt
    10621 ggttctgcag cagcagttgc agctggacaa attccagttt ctttaggtag tgatactggg
    10681 ggaagtatcc gccaaccagc agcttttaac ggcatcgtag ggatgaaacc tacttatggc
    10741 cgtgtctcac gttttggttt gattgccttt gcttcatctc ttgaccaaat cggaccaatg
    10801 acccgtacgg tgaaagataa tgcattagca ttgacagcta tcagcggtta tgacgaaaaa
    10861 gacggtactt cttcaggcgt ttcagttcct gattttgcag agggactgac aggcgacatc
    10921 aaaggaatga agattgcttt acctaaagaa tacttaggcg aaggtgtggc ccctggcgta
    10981 aaagaagctg tattgaaagc agcagaaaca ttccgttcat taggtgctgt tgtggaagaa
    11041 gtcagcttgc ctcattcaaa atacggtgtg gctgtttatt atatcattgc ctcatcagaa
    11101 gcaagttcca atttgcaacg attcgatggt atccgctacg gttatcgctc tgaaaatgtg
    11161 aaatcattag atgatgtata tgtcaactca cgttcagaag gattcggtga agaagtaaaa
    11221 cgccgtatca tgttaggtac attctcctta agcgcgggtt attatgacgc ttacttcaaa
    11281 aaagcaggac aagtccgtac attgatcaaa cgagattttg aaaaagtctt tgcagattat
    11341 gacttgatca tcgggccatc ttcaccaaca gttgcattcg gaatcggtga gaacattaac
    11401 gatccaatca caatgtacat gagtgacatc ttaacgatcc ctgtcaactt ggctggactt
    11461 ccaggtatgt ctgtaccggc aggtttatca gaaggattac cagttggatt gcagatcatc
    11521 ggtaattatt ttgatgaaaa aacgatgtat caagcggcat atgcgtttga acaagcaaca
    11581 gaattccata aacaacaacc agctatttta ggagggaaag acgaatgaat tttgaaacag
    11641 tcattggact agaagtccac gtcgagttaa aaaccaactc aaaaattttc tctccagcgc
    11701 cagctcattt tggtgcagaa ccaaacagca atacaaacgt gatcgactgg ggttatccag
    11761 gcgtgctgcc agtaatgaat aaagctgcgc tggaattcgg gatgaaagca gctttggcat
    11821 taaactgtga aatctctaaa gatactcatt ttgaccggaa aaattacttt tacccagata
    11881 atccaaaagc atatcaaatt tcccaatttg atcaaccaat cggtcatgac ggatggatcg
    11941 aaatcgaagt agaaggtaaa aagaaaaaaa tccggatcga acgtgtccat ttagaagaag
    12001 atgctggaaa aaacatgcat ggtattggcg gttattctta cgttgatttg aaccgacaag
    12061 gcacgccttt gatcgaaatc gtttctgaag ctgatatgcg ttcaccagaa gaagcatacg
    12121 cttatctaga agcattgcga tcaattatcc aatttacaga agtcagcgat gtgaaaatgg
    12181 aagaaggctc tatgcgctgt gatgccaata tttctcttcg cccttatgga caagaagagt
    12241 tcggtacgaa agcagaattg aaaaacttga actctttatc tttcgtgaaa aaaggtttag
    12301 cttttgaaga aaaacgtcaa gcaaaagttc ttctttcagg aggagaaatc caacaagaaa
    12361 cacgccgttt tgacgaaaca acgaataaaa cagttttgat gcgtgtcaaa gaagggtcta
    12421 gtgattatcg ttacttccca gagcctgata tcccgaaatt cgtgatcgat gacgaatgga
    12481 ttcaaaaagt aaaagctagt ttgcctgaga tgccagcttc tcgtcgcgaa cgctatatcc
    12541 gtgagtttgg acttccagag tatgacgcaa tggtattgac acttacaaaa gaaatgtctg
    12601 atttttttga agctgttttg gaaaatggtg cagatgccaa gcaagcatct aactggttga
    12661 tgggtgaagt ttctgcttac ttgaacagtg agaaactgga attagctgag acaaaattga
    12721 cacctgagaa cttagcagga atgattcgtt tgatcgccga tggaacgatc agctcaaaaa
    12781 tcgccaaaaa agtcttccgt gaattgatca caaatggcgg agaagcccaa gaagtagttg
    12841 aaaaaaatgg ttgggtgcaa ttatctgatc cgaacaaact actgccaatc attaatgaaa
    12901 ttttggataa caaccaacaa tctgtcgatg atttcaaaaa tggaaaagat cgtgcggttg
    12961 gcttcctagt cggtcaaatc atgaaagcaa ctaaaggaca agcaaatcct ggtgtcgtga
    13021 ataagctact taaagatgaa ttagcgaaac gatagaggct gaaaatgaaa aaagcacgcg
    13081 taatttataa ccccacctcg ggaaaagaat tattgaaaaa aaatttggcg gatatcctac
    13141 aggcattaga agaagcagga tttgaagcca gcgcctatgc aactacacca gaaccagatt
    13201 cagccaagaa tgaagcatgt agagtcgcag aactaggttt tgatctagta gtggcagctg
    13261 gtggcgatgg cacaatcaac gaagtggtta atggaatcgc tccgcttgaa caccgtccta
    13321 aaatggccat catcccagct ggtacaacga acgactatgc tcgagcatta aaaattccta
    13381 gagataatat taaagcagca gcagaagtga taaaaaaaca ccaaaccgta aaaatggaca
    13441 tcggcctttc tgataacagt tacttcatca atatcgctgc aggtggctat ttgacagaat
    13501 tgacttatga agttccatca gaactaaaga gtatttttgg ttatcttgct tatcttgcga
    13561 aaggagcaga gatgctccct cgagtaaagc caatcaaaat gcgcttgaag tatgatgaag
    13621 gggaatatat cggaaatgct tcgatgtttt tcctagggct gacgaactct gttggaggat
    13681 tcgaacagat cgcgccagat gccaaattgg atgatgggaa gttttcttta atcattgtga
    13741 agacagcaaa tatctttgag atcttgcata ttgcagcact catgctgaat ggcggtcgac
    13801 atgtcgatga tccaagaata atctatacca aaacaagcag tttgttcgtt gaaacagaag
    13861 atgatatcaa tcgaccagta atgatcaact tagatggtga atacggcggg gaagcaccga
    13921 tgcatttcca aaatcttcat caacacattg agttctttgc caataccgat cagattcctg
    13981 atgaagcaat cacaggtaca gatgaagaag aattagaggt tgtcagcaaa gaattcgtaa
    14041 aagaagtcga acgtttaacg gatgaagata tcgacggtga cggaaaaata gcagacgaaa
    14101 cacctaatca aaagaattaa tcaaaagccg gtgctgaacg tagtaaggtc agtgccggtt
    14161 ttagttgtgt aattagctga tttatagtag agttcagtag gattgaaaaa taggcttaaa
    14221 ggagcaaaat atgacagaac agcaaatagt aaagaaaaac gaacgatata cagtcgaaat
    14281 cgtcgacttg agttatgaag gaatgggggt agcaaaaatc gatggttatc cattatttat
    14341 cgaaaatgca ctccctggag aagaagtaga aatactagta gtaaaagcag gcaataagtt
    14401 tggatatgga aaagtcgaaa catttattac acaatcacct gatcgtcagc ctgtagaaaa
    14461 caatgcatta cttcgcatag gtatcgcacc tttagcccat ttgaagtatg aacaacagct
    14521 tgtcttcaaa caaaaacaag tagagcatgt aatggcgaag attgctaaga tgccagaagt
    14581 tcctgtactt ccaacggtcg gaatggcaga accattcggc taccgtaata aagcacagat
    14641 ccctgttcgc cgtatcgatg gacagttagc caccggtttt tacaaaaaga atagccatga
    14701 tctagtccct atcgaagatt tttatatcca agatcctgtc atcgatcaag caatcaaagt
    14761 agtcagagat atcttgcaac ggttccaagt acgaggatat aatgaagaaa aaaatgaagg
    14821 acagcttcgt cacatcatta tccgtcgtgg tcattattcc catgaaatga tggtcgtttt
    14881 agttactcgt aaagaaaaat tctttaaatc caaggaaatt gctgaagcta ttcatgaaga
    14941 attgccagaa gtggtttcgg tcatccaaaa tatcaatgaa gaaaaaacaa atgtgatttt
    15001 aggagacaaa gaaaaagttt tatatggcag agactatatc gaagatcagc tattaggaaa
    15061 aacctatcga atctcagcaa agtcatttta tcaagtaaat actcaacaag cagagtatct
    15121 gtaccaaaca gccatcgagt ttgccgagtt agataaaaaa gacattgtga tcgacgctta
    15181 ttcaggaata ggtacgattg gcttgtcttt agcagacaga gtggataagg tgtatgggat
    15241 ggaagttatt ccagaagcaa ttgaagatgc acagttcaat gctttaaata ataaaatcga
    15301 aaatgttcat tatgaagtcg gaaaagcaga aaaagttatg aaaaaatggc aagaaaaagg
    15361 gatcaaacca tctgtgatcg tagttgaccc tccacgaaaa ggattagatc atcattttat
    15421 tgaatcagca atcgaaatgt caccagaacg aatcgtctat atctcatgta atccggcaac
    15481 ttttgcaaga gatgctaagt tatttgcaga agcaggttat gccgtgaaga aagttcagcc
    15541 tgtagatctt ttcccgcaaa cccatcatat cgaattagtc gcttcattcg ttaaaggaga
    15601 ataaaaatgg accaaaaaga aaaaatgaaa gaattgatca aaaagaaaca aggtggaggc
    15661 cgatccaaac aaatggaaac acctaagaat gatcgtaaaa acatgagaaa aggccctaaa
    15721 atctttaata aatagacgtg ctaaagaaag actaagaagt gttaactttt gttattgttt
    15781 cttagtcttt tta
//
LOCUS       DAZAOG010000058        15756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000058 DAZAOG010000000
VERSION     DAZAOG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.058"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACHDLD_001506"
     CDS             138..1475
                     /locus_tag="ACHDLD_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN3363213.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACHDLD_001507"
     CDS             1523..3652
                     /locus_tag="ACHDLD_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN3363214.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACHDLD_001508"
     CDS             3627..4316
                     /locus_tag="ACHDLD_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363215.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACHDLD_001509"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACHDLD_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACHDLD_001510"
     CDS             complement(5546..5740)
                     /locus_tag="ACHDLD_001510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363216.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACHDLD_001511"
     CDS             complement(5790..6230)
                     /locus_tag="ACHDLD_001511"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN3363217.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACHDLD_001512"
     CDS             complement(6234..7079)
                     /locus_tag="ACHDLD_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN3363218.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACHDLD_001513"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACHDLD_001513"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN3363219.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACHDLD_001514"
     CDS             complement(8703..9650)
                     /locus_tag="ACHDLD_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3363220.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACHDLD_001515"
     CDS             complement(9873..10223)
                     /locus_tag="ACHDLD_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN3363221.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACHDLD_001516"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACHDLD_001516"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN3363222.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACHDLD_001517"
     CDS             11646..11966
                     /locus_tag="ACHDLD_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3363223.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACHDLD_001518"
     CDS             11988..12452
                     /locus_tag="ACHDLD_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3363224.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHDLD_001519"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHDLD_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN3363225.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACHDLD_001520"
     CDS             complement(13301..14590)
                     /locus_tag="ACHDLD_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN3363226.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHDLD_001521"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHDLD_001521"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN3363227.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4953 a         2915 c         3226 g         4662 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaa
//
LOCUS       DAZAOG010000059        15687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000059 DAZAOG010000000
VERSION     DAZAOG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.059"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACHDLD_001522"
     CDS             68..751
                     /locus_tag="ACHDLD_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN3363228.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACHDLD_001523"
     CDS             753..1589
                     /locus_tag="ACHDLD_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3363229.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACHDLD_001524"
     CDS             1600..3354
                     /locus_tag="ACHDLD_001524"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363230.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACHDLD_001525"
     CDS             complement(3630..3971)
                     /locus_tag="ACHDLD_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3363231.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACHDLD_001526"
     CDS             complement(4039..6210)
                     /locus_tag="ACHDLD_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN3363232.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACHDLD_001527"
     CDS             6421..7278
                     /locus_tag="ACHDLD_001527"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3363233.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACHDLD_001528"
     CDS             complement(7349..8206)
                     /locus_tag="ACHDLD_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3363234.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACHDLD_001529"
     CDS             complement(8191..10893)
                     /locus_tag="ACHDLD_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN3363235.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACHDLD_001530"
     CDS             complement(10906..12195)
                     /locus_tag="ACHDLD_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN3363236.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACHDLD_001531"
     CDS             12345..13391
                     /locus_tag="ACHDLD_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3363237.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACHDLD_001532"
     CDS             13393..15546
                     /locus_tag="ACHDLD_001532"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN3363238.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DAZAOG010000060        15604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000060 DAZAOG010000000
VERSION     DAZAOG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.060"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..618)
                     /locus_tag="ACHDLD_001533"
     CDS             complement(<1..618)
                     /locus_tag="ACHDLD_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3363239.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITS"
     gene            complement(767..1480)
                     /locus_tag="ACHDLD_001534"
     CDS             complement(767..1480)
                     /locus_tag="ACHDLD_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3363240.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            1655..3079
                     /locus_tag="ACHDLD_001535"
     CDS             1655..3079
                     /locus_tag="ACHDLD_001535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3363241.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            3378..4085
                     /locus_tag="ACHDLD_001536"
     CDS             3378..4085
                     /locus_tag="ACHDLD_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN3363242.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4072..4395
                     /locus_tag="ACHDLD_001537"
     CDS             4072..4395
                     /locus_tag="ACHDLD_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN3363243.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(4484..5521)
                     /locus_tag="ACHDLD_001538"
     CDS             complement(4484..5521)
                     /locus_tag="ACHDLD_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3363244.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5842..6825)
                     /locus_tag="ACHDLD_001539"
     CDS             complement(5842..6825)
                     /locus_tag="ACHDLD_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN3363245.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7240..8010)
                     /locus_tag="ACHDLD_001540"
     CDS             complement(7240..8010)
                     /locus_tag="ACHDLD_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363246.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8003..8938)
                     /locus_tag="ACHDLD_001541"
     CDS             complement(8003..8938)
                     /locus_tag="ACHDLD_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363247.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9254..10903)
                     /locus_tag="ACHDLD_001542"
     CDS             complement(9254..10903)
                     /locus_tag="ACHDLD_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN3363248.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11020..13623)
                     /locus_tag="ACHDLD_001543"
     CDS             complement(11020..13623)
                     /locus_tag="ACHDLD_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN3363249.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(13635..14684)
                     /locus_tag="ACHDLD_001544"
     CDS             complement(13635..14684)
                     /locus_tag="ACHDLD_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3363250.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(14710..15024)
                     /locus_tag="ACHDLD_001545"
     CDS             complement(14710..15024)
                     /locus_tag="ACHDLD_001545"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN3363251.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15040..15486)
                     /locus_tag="ACHDLD_001546"
     CDS             complement(15040..15486)
                     /locus_tag="ACHDLD_001546"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN3363252.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4659 a         3216 c         2600 g         5129 t
ORIGIN      
        1 cgaggtaatc gtaataatga ttgggattaa tacattaaaa gactttaaaa cagcgggagg
       61 aatatttccg gatagctgaa tctgtagttt cttattatta gaaagtttaa taaataattc
      121 tgtagccgct aatccaacaa atatacctac aaacattcct gaagctcctg tataggatat
      181 aggtatcaca cccgtcactt caacagcttt atccgcattt tcaggagtaa acatcatctg
      241 cataggtgtt aatacaacaa atgtcgaaat agcaactaat accgctgcaa tatgatttgt
      301 ataatttctg cttgcacaca tatgataaga tactgctgct acaactaata atgtagttat
      361 gcttaatgta ccattggcaa ttgaagtacc aatttgttgt aacgtggtca atgtactagt
      421 atcgacaatt tcccccatga atcctgttgg ttctaagatc actgaattaa taagtgtcac
      481 aaatcctgct aacatcataa atggcataaa tgtagcaaac gcatctcgca aagttcttaa
      541 atggatctga ttccccaact tagtcgcaac tataccaaat ttttcaaata caatgtcctt
      601 cctgctactt ttgcccatat tttttctcct ttatgcttat tgtaatcgat tactttttcc
      661 acccaaataa tacatcactt atcaaaaaaa gtaaatactt ttaattaaat atatatatct
      721 tttttagtat aaaaaaataa gctctgaaaa aataattttc ccgagcttat ttttcaacaa
      781 aaacagcttc aaaaacaaag tcttgatata aatgtctcga taccgaatac tcgaaaggtt
      841 taccagacga tagagcaaca actttgtcta tctcaatcat aaattcacct ggatctattt
      901 ttaatatttt tgcgtcaaat tcattcgcag tatctccttt gatacgtatg tgcgaagtct
      961 ttatttccag acccagttca tttgtgatat aagcatatac agatttcttt aaatgttttg
     1021 gttccaagcc tggtatgact gctaatggca tgaatgtttg ctcaatcgag tatggttgac
     1081 aatcaataaa acggcttcgt acaatattat atacatagtc ttttacactg atacttaatt
     1141 tatcagctac ttttttcgat ggaagttcaa ttgagaaagt atggatttct gaatctacgt
     1201 gttcttcacc aaccacgcta aacaaacctt cgtttggacc aagaactcgt gagttatcaa
     1261 ttggtgttct gacgacttca gtcccatacc cacttctact tcgcaaaact ccttcggaaa
     1321 caagtaagtc tacgcctttc tttactgtta acttgcttgt atgataaata actgccaatt
     1381 catctcctgt aggaagtttc tcaccaattt tatactctcc atttacaatt tttttcttta
     1441 tatctaagta aatttccata tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa
     1501 acatatcttc atctatactt taacataaat aaaccgataa aatccacatc aaaattatac
     1561 acacaataaa aaagatatat atatttaaac aaaactattt actttaagtt gtaagcggtg
     1621 ttatcattaa gtggaaacag gaggtaaaaa aattatgaca actatgaaaa agctaacttt
     1681 tccagatgat ttttggtggg gttccgcttg gtcagctgag caagcagaag gaaaaggaga
     1741 aacaggaaaa gcagagacta tctgggatcg ctggtttaaa gaacaacctt accggtttta
     1801 tcaacgaatc gattcctcta ttacaaccga tcacatccat caatataaag aagatataga
     1861 actaatgaaa aaaactgggc ataactcctt tcgcgtttct atttcttggg cacgaatgtt
     1921 tccaaatggc ggtaccggaa aagtaaatcc caaagccatt tctttctatc gaaaattatt
     1981 ttccgaaatg aataataacg gaatcaaagt atttgccaac ttatatcatt ttgacatgcc
     2041 tgctactctt caagataaag gaggatggga atcacgtgaa gtagttgatg cttatgttca
     2101 ttacgctgaa acttgtttta aagaatttgg tgacttagtt tgtcattggt ttaccttcaa
     2161 tgaaccactt gggcctattt taggtactta tcttgaagat tttcattatc caaacctcat
     2221 tgactttaaa cgcggagcgc aagcagcatt cttcactata cttgcacacg caaaagcaat
     2281 acaagtattt aaagaactag ctcttcctag taaaataggc gttattctaa atttgagtcc
     2341 tacttttcct cgtagtcaaa atcctgctga tgtgaaagca gcagaaatcg ctgatttatt
     2401 ttatacgcgg agctttttag atcctatggt taaagggaat ttttctaaaa agcttatcct
     2461 gttattacaa gaatataatc agcttcctga agattatacg aaagaagatc ttgtatgtat
     2521 ctacgaaaat accgctcaaa ttcttggact aaactattac gagccacgtc gcgtcaaatc
     2581 tcgttcttct atggtggata catctagtcc atttttacca gagtggtttt tcgaaccaca
     2641 tatcatgcct ggacgacgaa tgaacgaata ccggggatgg gaaatttacg aaaaaggaat
     2701 ttatgatttg tgcatggata tcaagaacca ttatggaaac atcgaagcat ttatctcgga
     2761 gaacggaatg ggcgttgcgg atgaagaacg tttcatggat gatacagggc aaataattga
     2821 tgattatcgg attgagtata tcaaagatca tcttgcttac ttatggaagg caataaatga
     2881 aggatgtaac attaaggggt atcatctttg gacatttatt gattgctggt catggatcaa
     2941 cgcctataaa aaccgatatg ggctggtttc actcgacctt actactcaaa aaagaacaat
     3001 taaaaaaagt ggcgaatttt acaagcaatt aagtgataca aatggattta gatacgatgt
     3061 agacttgtta gttgaataat attctaaaat tccacttgaa gtgaacacat tagaaaacaa
     3121 gaataatgag aaataccaat aacaaatact ttttcttcat acttcattta tacttgtcag
     3181 tataagagac tgtgacaaat gtagtgatcc tcagaagtta ggtctggaaa tctaacttga
     3241 gaagattttt ttaaactacg tccagtcttt tttatttgtt taaaattgaa gttgacaagt
     3301 cttcattaaa aatttagaat attcacaagt ttgacgacaa ttatgaaaat ttatgtcgtg
     3361 ctaaaggagt tttgttattg gatgaaaagt tagatataaa aactgcgatc aaagacacgt
     3421 tacctaccgt ttttggttat atcggtattg gacttgcatt tggtatcgtt gggaaagctg
     3481 ccgggtttca tccgctggtc gtcacgttga tgtccctgct gatctatgct ggttctgccc
     3541 aatttatcac ggtcagcatg cttgctagcc atagtccact gctctccatc gttctctcga
     3601 cctttctagt caattcccga atgattctga tgagtatgac gattgctcct tatttcaaga
     3661 aaaatcggct gcttcaaaat ctgctgattg gcacattgct gacagatgaa agtttcgcac
     3721 tgggaatgaa taaattgaac tatactggac aaaagttaaa ctttcgttgg atgaatacag
     3781 ctaattggat ttcttatttg acgtgggtgg gttcttctct tgttggcgca cttttaggga
     3841 attttattac tgatccgaaa aagtttggtt tggatttcgc catcgtcgct atgtttatcg
     3901 ggttacttta tcttcaagtc atttctgata gaaacacctc taaacgatta cagttgatcc
     3961 tgattggtct cactttgatt ctggtatatg tcggactgat ttttatccca agtaacttag
     4021 tgattttagt tgtaacactg attgggtgcg gattgggggt gtggatcaaa catgccttct
     4081 tttgagttta cattgctgac tattttaggt tgtactttag caacttggct ttctaggatc
     4141 ctaccatttg tcttactgaa aaaattcgat ttgccgcaac cgttgcttga gtatttaagt
     4201 tttgtcccga tcgtcattat gtctgcttta tggttcagca gtctatttac ccaaaatatc
     4261 ggacatctgc cgcagatcaa tctcgaaaat gctttggctt cagttcctac acttttagct
     4321 gctattcttt caaaaagctt attggtgatc gtcttggtag gaattctttc cctttcgttg
     4381 attcgcttat tataaataaa aaaacaagag cttatggcaa attactgtca tgcgctcttg
     4441 tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac
     4501 cactaacaac gacttttcct aatatatgtc ctgtttctac gtctttggtt gcttgtttca
     4561 agttcttcgc attcatccca tctgaataga ccttgctcaa tgtactgcgg ataatccctt
     4621 catttgctaa agcagcaatg tgcgctaaag cttctccttg cgtttcgatt tcatagttat
     4681 agtctgtttt agcgaacata tactcccagt cgaagctgat agattggttt ttccaaacac
     4741 ctatatctaa cggctctttg atccctacga tcgttcccac atgtccaaat ggtcggacaa
     4801 ggtgtttgat ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca tactctactt
     4861 gatcgattcc taattcacga aaacttttac ttaagtcgtg atgataatcg attggatagt
     4921 ctacacctgt ttttttcagc cattcaaaat tcttcggact tgcagtcgcg tagacttcta
     4981 ggccagccca atgcgctagt tgattcaaaa tcgaaccgac gcccccagct ccgttgatca
     5041 ctaagatttt tttcccgcgg ttggcatttt cttctggagt cagaccaaat ttttcaaata
     5101 acaattcata agcggtcaat gaggtcaacg gcaaggctgc ggcttcttca tctgaaagat
     5161 tttttggtgc aagagcgaca atacgttcgt caaccagttg gtattcttga ttgcttcctg
     5221 ctcttgtcgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt tgcacttcat
     5281 cacctacagc aacaactttc ccgactccat caaaccctaa gatacgtaaa gcatttctta
     5341 tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg ttgactttta
     5401 ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata aagctttttg
     5461 gatcatcgat cgacaaccct tcataaaaac cgactacctt cattaattcc ttttctgaca
     5521 tacgaattcc tccttagctt catcaagttg gagcatccat acagttttca tttcaatata
     5581 tatagtatgc tatttgttcc acttttattc atcataatta ttctcttact taaagtaaac
     5641 tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac aacctctttt
     5701 aaacggtgtt caatcaactg accctcggta ttcgctcaaa aaacgacaaa atatgaggag
     5761 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac gacctctaat
     5821 tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa
     5881 ggtgtctaag atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa
     5941 gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat
     6001 ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc
     6061 gatcagttct ttttctgtat gttccctcgg ttctttttca aatgcataag gattcttgaa
     6121 aatcccccgg ccaatcatga tgccatctac gccatatttt tcggccaatt cttctcctgt
     6181 ctggcgatct gggatatctc cgttgatcgt gatcagtgtt tgcggagcga tttggtcacg
     6241 taaagcaatg attttaggga tgacttccca atgtgcatct actttactca tctctttacg
     6301 cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc
     6361 ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc
     6421 agctttagcc gcttcaatca attgtgccgc tacttctggg cgaaggatca agccactgcc
     6481 ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa
     6541 gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat
     6601 atgggcaacc atcggctgtt catcttctgt aaaggtcaat cttcctcgta cactgtcttt
     6661 tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc
     6721 tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa
     6781 aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc
     6841 cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa
     6901 caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaagactg
     6961 tgacaaaaat cttatcacaa ttccttttcc ggaataaatg gtgttcaaaa gcttgcatct
     7021 gcggtaataa gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
     7081 ttacttgatg cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga
     7141 ccctcggtat tagctcaaca aacgacaaaa tatgaggaac tattttcccg tttgtttctc
     7201 taatactcag gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc
     7261 tttattgatc aaataccaag taacggcgta caaaacaacg atgaatagca caagtacgat
     7321 catcgatgtt gctactggca catcgattgt tccaaggaat ccgtaacgga aaccggagat
     7381 catataaacg atcggattga ctttcgaaac agcttgccaa aatggcggca acatagagat
     7441 ggcatagaaa acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga
     7501 tacatcgtca taagactgag caaaaatccc gttgagcagt cctgctaacg aaaacaagat
     7561 tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa
     7621 gaacaaggaa atgatcgtta cgagagagcc gacgagaata ctgcgaccca ctccgccaat
     7681 cacaaagccc caaatgatca catgcgtagg aacaggcgct accaagattt cttcgatgtt
     7741 tttctggaat ttttgagaga agaaggacga agaaacgttg gcatacgaac tagtgatagc
     7801 agacatcata atcaaaccag gcacgatgaa ttccatatat gaaaaaccac ccatgtcacc
     7861 gatccgacca ccgatcattt ttccgaaaat aacaaagtac aaggatgtcg tgatcacagg
     7921 cggaaccagt gtttggaccc agatacgcaa atagcggttc gtttccttga tagctaaact
     7981 ttttaatgca gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg
     8041 tgattttcaa aaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga
     8101 ttccttgtct tgtcagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga
     8161 cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc ttcaatcaca ggtttggtct
     8221 catatggcgc taaatcaaag ataaatgttt caaattgcag tttggataga agctctttca
     8281 tactagtatt ctcgatcagt tcacctgatt ggatgatccc gatgttgcga caaagcattt
     8341 cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc
     8401 ggagaaactc ccacatctcg cgtctcaatt caatatcgac tcctgctgtt ggttcatcca
     8461 agatcagcaa acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc
     8521 ccccggaaag catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat
     8581 atttttcgct gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca
     8641 cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa ctcttgcggg actaatccaa
     8701 tctgttgctt tgctcggact agatccgtat ccagatcata accgaagaca ttcacttttc
     8761 cagatgtttt attgactaaa gaagtaatga ttccgatcgt tgtagacttc ccagctccat
     8821 ttggtcctaa tagcgcatag aaatctcctt ctttcacgga aagatcgatt cctcgcaatg
     8881 cttcgacccc acttccataa acctttttca aattatttat ttcaagcgca tttgccattt
     8941 atttcctatc tccttataga catgattctt tattgtatca tggaatacag cagatgaaaa
     9001 aacaacttgg ttccagcgaa aaatcaggtt gctgggctca ggaaaatctc ctaatgaaag
     9061 acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa caaatcgaag ataaaggatt
     9121 actattcata tgtgcaacaa aaggagcgtg agacaaaagt aaagaacact tttgtctcac
     9181 gctctaaaca cgtataaacg gcggaagcaa aagccaagat ttttgatcca ataaatactt
     9241 tcgatcactt cgcctatccc aatacatgcc actgccgctt ttcatcaaat aacgcggctt
     9301 ccgtttttgc ggtttctact aaagtctctg ggtaatccaa aactttcagc aaggcaatcg
     9361 catttcgggt ccttgaaggt ccttctttta atttgaaatc aaaactgatt ccttttcctt
     9421 ctgttacttg ttcttcaaag tggacattct cacaataatt ctttaaaatc tctgtcaatt
     9481 cgatatcatg tgtggcgaca aatgctaaag atggatactc cgcaagccac cgaacgactg
     9541 aagcggaaga agcaatccgt tcgatcgtat ttgtcccttt taaaatctca tctacgaagc
     9601 agtagcagcg ttctttggaa gccacttggt ccaacaaacg tttgatcgat ttgatttctg
     9661 aaacaaaata actgtcgcct tcaaataaat catcttcgat tgccatggaa gtcaacacat
     9721 gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac
     9781 agctgatggc tatacttttg acatacgtag attttcctga tgcattggaa cccgtgacta
     9841 atgtattctt ctgccagttg accggattca ctaccgcttc ttttaacaat ggatgataga
     9901 tatctttcgc tgtcacaccg ccctcagtaa actctggtcg gcaagtgatg ggcatataag
     9961 tacggtaatt caatattgca gcggctactt ctaattctcc cagcaattcc cacagcttga
    10021 tggcttcagt tgaatgcttt tctaatttag tgagaacgaa atgataggag ataaatggca
    10081 gcatgaacat gatgttgaca tattcaaaaa tcaactctgc ttcactccca ctcttcatac
    10141 gaaatgaaaa acctaattta gggatgtctc ttaatggttt agtttgctgt ttcagctctt
    10201 cttgaagtgg tgtcggcagt tttgctagct gattggcggc tgaaatcgtt gaaaccaaat
    10261 aactcatgct attcagctct gtttctagtg tttgtttctt cactagataa tagaccgtat
    10321 tgaatactaa cccaccgatt gccaaataga ttcctagtac ttgtcctaaa agaagcaaac
    10381 ccaaacccac cagcggaagc aagccaagaa aaataaataa acctacgcgt ccaatctttt
    10441 tactttttcc atccgctaga tattgttttg caaaattatg atcttttttt cctaaacgag
    10501 cgaattgata ttggattttt tctctcagtt gaggattttc ctgataaaaa gcaatcagtt
    10561 tttctaactg ctgatcttca ccaaaatcaa agctgcgtaa acgttgatac aacgcttctg
    10621 aacctacact agaataggta ctgttgattc cttcgaatac ttcgaacata tccaggtcat
    10681 accaagtaag atcatcgatt tcgctatccc atgaacggaa cttcttctcc gtgagccatg
    10741 cttccttcaa gctttcttct ttatcaaatc tcggttgata aggaattttt tcccatttgc
    10801 tacgaactgt ttcctttaat ttcaaacgtg aaaaaatgtc taaagcaact aataaaataa
    10861 cagctgcaat aataattaaa acgataaatt ctctttgacc catatccctc tccgttcctt
    10921 tatttgttag ctttagtata gcactgttgc gctatttgaa aagagggtaa aaaaacacct
    10981 ttcaaaataa aaggtgtttt tttcttgtat attacatatt tatctctcat ttatcaaaat
    11041 tgacaaataa tcatagggag gaattgttgt taattcttgt ttattctcca gtgcattgac
    11101 aactgtccca agggaaagga tagcctcagg tactattttt tcttgttctg ttggattagc
    11161 taagcgaaga acaaatcctt gttcatgaaa ataagaagga taaatcgatg atacaattaa
    11221 gtcttcaggt aattcaatca atgaaaattt acgaggaaca tcaagttcat tttgtaatgg
    11281 ccaaatcttg ttatctaacc tgtgaataaa tacattcagt ttctgctttt gataagaaat
    11341 agattgttct aaccgttttt ctgcctgttt tgccactttg aactcattaa atcttccttc
    11401 ttctacatta aatccaaaag tgactttata ctcacctatc tcttgagcta gcggtgtttc
    11461 catcatgaca tgcccttcat ttgtcgtatc tcctgaagct cgcccaggtc gccaagcaag
    11521 attaggtttt ccaagttccc ctgttgttga aaataaggtt atgattaatt gattttctat
    11581 acgttcatac tcttttaaac catctgcata aaacgtaaaa taatgatcat tttctgccac
    11641 agagacactc ttatcgaata tttctaaatt aacaggtttc tctaccatag tttctgacca
    11701 attcggagga atgacatcag gggtattttc aataaatccg ttttgaattt gtgcaattga
    11761 tttttcagat ttatctaaaa cattaattgc taagcgcata cgatgcgagt aaacagtatt
    11821 atgaattttg agtgtgcctt caacgatttg actttctccc agactgaacg atatttcata
    11881 agatatttct tccatttcgg gtttttcaga taaacgatct tttaaatctt taggaagttg
    11941 agcttttccg taaacaacta atgtttcttg taaagaatct ttatatactt tagctgtttc
    12001 taattttaga gatagttctg tatctccttc taatggtgaa taatcatacg tatcgccatc
    12061 atttgctgaa tcgatcaaat gaacgaaatc aagatattgt ctatctccaa ctatcaaatt
    12121 caattgccca tctttaaaag aaagtttata tttattgtta cttatctcag ttttgtctat
    12181 ctcgattgct gtttctaatt tttcattatt tttctcaaat tcaacaacag tgtatcctag
    12241 tgctggcaca tttacattta tttttatgtc aagttcataa taagctggtt catcaaaata
    12301 ttcgaaacct gtagcaatca tccgataagc atttttccgt tctggaaaat aaacttcttt
    12361 ttcaatgata ttatagggac tacctttgaa gtgtattttc tttgaatcag ctactacatg
    12421 cacgattttc gatccactat aattctcagt cgttgtgttg aaaacaagga cttcattcga
    12481 ttttagcttt aatgcatcag ccatccgttt tacaataagg ttttcgattc cgtcagctat
    12541 ctcattagct tctttgattc gatggaaaat atcttccgca acattatctg aaacacagcc
    12601 accaatacta tcatgagcct ggttttgcaa aatctttttc cataaccgac gaagtaggcc
    12661 tttactgata tggataccat ttctttctgc aatcagcatg agcggctcaa ctcgttttag
    12721 aattttttgt tccaatttaa aattttctat tttcattttt gttcttactg agccaataga
    12781 acgatgaaca cgagagtaaa ccggctgacg aagttcacct ttatattcag gtaattcttt
    12841 tttgtttctg ataacttcta cataagaagg ataatctgaa ataatattgt ggaatttact
    12901 gtatttattc aacttctcta cgacttgatc aaaatttaaa atcattgact tttgatctac
    12961 acccgaaggt actaaaatat ctttgttttt tccttgcata tgaagcattt caactgcttc
    13021 atccaacttt tcttttgcaa attcaggaat atctttaatt gcgtctaagg tcatcattcc
    13081 agtcatataa ccaaatggaa aattcatggc ataaacaaat ttatctccca atcctttcca
    13141 gataaaataa ggagatggaa ccatcgtttc aaagtttaat cctcgccaac acatgaaatt
    13201 atcgatttct acttgatgca aaagtgtagg caaattcgca ttgaacccaa acgtgtcagg
    13261 caaataccct accatcattg gatctccata tttaaggatc gtatcactga ttccaatcat
    13321 taagtttctt aaaattgatt cagcatccac taataaggca tctgtttgtg cataccacgg
    13381 tcccacgaat agttgtcctg ttttcatcag tcgttggata accttaagct tttgcggatt
    13441 gatctccaaa tactcatcga cgattgaagt ttgtccatct aaacaaaagt ttactttagg
    13501 gttcctttcc aattcatcta gaacttcagt aaagacctga tcagacaata cgatggcatc
    13561 ttcatttgta aaataccatt ctctgtccca gtgcgtgtgt tgaattatat gtgcatttac
    13621 catatcagat tctcctactt tccttcttct actgaattta cttgttgttc ttttttatac
    13681 ttgatggatt ttagtgcatt gaccattaac gctgtaacca ctcccccaat aacaattgat
    13741 actaaatata aaggtatatt cgtaacaaaa ggaattgcca taaaggttga aagcattgtt
    13801 tcttgagtaa caccaaaaaa tccatttaga ccacatgcta ctgcagtgcc caccacgatg
    13861 cttggtatta ctcgtaaagg atctttcacg gcaaacggga tcgtcccttc agtaatcccc
    13921 acaaggccca taatcatagc tgtctttgca ttatctcgtt ccgattcatc ataaagtttt
    13981 ttgtttaaaa ttgttgctaa aaacattcca aatggcggaa tagcaattcc taataacatg
    14041 gctgtactag gtccaaagct tccgcttgaa aaagaagcaa ccccaaaagc atatgcgatt
    14101 ttattgatag gtcctcccat atcaaacgcc atcattcctc ctaaaatagc cgacatcaaa
    14161 gcagcatttg ctcctgacat gccttctagc caattagtca gtcctgttgt cattgcttga
    14221 ataggcgtag ctagtacata ccataatatg attccaacaa ctaatgtagt aacaaaagga
    14281 atcagcataa ttgattttag actaataact tgcatcggta cttttacttt tttcaatgca
    14341 ttagctaaat agccacaagc taatccagca accattcctc caataaatcc cataccaaga
    14401 ttttgacaca tcatcccagt gataaaagct ggtgctagac cgggacggtc agcaattgaa
    14461 tatgcgatat aggctgaaat catcggtacg ataaactgta atcccgtctg accaatcatt
    14521 cgcaggactc cccagaatgt atccgtctgg tcccaaacac ctgtgccacc aaaaatgact
    14581 gcaatagaag ttagtaaccc tgctgctacg acaagcggga tcatatagga aaccccactt
    14641 tggaaatgat ttagaatttc tcttccttgt ttcttgaatt ccattagtaa ctcccccacg
    14701 atatattttt tatttgttaa ttaatgcaat tgcttcatca atcgtctctt caccatcttt
    14761 tactacctct gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga
    14821 aacatttgta tctactgcaa atatgacaac atctgcctcg gaaacatccg aaattgaaag
    14881 tttgttttct atccccatag ccccctgtgt ttctacttta atatctaacc ctttcttctt
    14941 cgcaaacttt tctaaattag cttttgccat ataagtatgg gcaatccctg cgatacatgc
    15001 tgttactgct actatttttg tcatatgaaa ttcctccttt taaaatgata gagcttttac
    15061 caattctttt ttatttctcg aatcttttat tcttttaaca aactcgtcat ccatcaatct
    15121 tccagataat tctgctaaaa tacgtaaatg attagtacct ttatcagtgt cttttattgc
    15181 taacaaaaag acgagatcaa ctggttgatc atctaatgcg ccccattcaa cagaccgctt
    15241 taacttggct acagccactg ttgattcaat tactgctagg cttttgccat gaggaatggc
    15301 gatatttttc ccaattcctg tggtcatatg gttttctctg tcaaatacgt ctttttgaaa
    15361 ttgtttgtgg tcagacacaa acccattagt ctctaacaat ttcgataatt tttcgactac
    15421 acttttttta tcagatgcat ttaaatctat cactatatta tttatattga ttttttcact
    15481 gatcatttat aagccttctt tcaatatata aactaatttt tcaaacgacg aactcgtttt
    15541 taaatttgct aataactcat gattcgaagc gatttcattg aatgtttcat aaattttatt
    15601 caat
//
LOCUS       DAZAOG010000061        15084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000061 DAZAOG010000000
VERSION     DAZAOG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.061"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1246)
                     /locus_tag="ACHDLD_001547"
     CDS             complement(167..1246)
                     /locus_tag="ACHDLD_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3363253.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            1354..2805
                     /locus_tag="ACHDLD_001548"
     CDS             1354..2805
                     /locus_tag="ACHDLD_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3363254.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3154..3786
                     /locus_tag="ACHDLD_001549"
     CDS             3154..3786
                     /locus_tag="ACHDLD_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN3363255.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4374..5813
                     /locus_tag="ACHDLD_001550"
     CDS             4374..5813
                     /locus_tag="ACHDLD_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3363256.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6108..6326
                     /locus_tag="ACHDLD_001551"
                     /pseudo
     CDS             6108..6326
                     /locus_tag="ACHDLD_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6575..6790
                     /locus_tag="ACHDLD_001552"
     CDS             6575..6790
                     /locus_tag="ACHDLD_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363257.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(7007..7263)
                     /locus_tag="ACHDLD_001553"
                     /pseudo
     CDS             complement(7007..7263)
                     /locus_tag="ACHDLD_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7450..8361)
                     /locus_tag="ACHDLD_001554"
     CDS             complement(7450..8361)
                     /locus_tag="ACHDLD_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3363258.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8380..9384)
                     /locus_tag="ACHDLD_001555"
     CDS             complement(8380..9384)
                     /locus_tag="ACHDLD_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN3363259.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9381..11504)
                     /locus_tag="ACHDLD_001556"
     CDS             complement(9381..11504)
                     /locus_tag="ACHDLD_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3363260.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11509..13956)
                     /locus_tag="ACHDLD_001557"
     CDS             complement(11509..13956)
                     /locus_tag="ACHDLD_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3363261.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13943..14332)
                     /locus_tag="ACHDLD_001558"
     CDS             complement(13943..14332)
                     /locus_tag="ACHDLD_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3363262.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14392..14895)
                     /locus_tag="ACHDLD_001559"
     CDS             complement(14392..14895)
                     /locus_tag="ACHDLD_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN3363263.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4525 a         3032 c         2826 g         4701 t
ORIGIN      
        1 acaggtaact catttgtttg ataccgtaac tgcctcgtta ttctaaatca gtgaggcctt
       61 gataatgaac tgaggtatga tagtcttcag acgattctgg ccccaggcca gctttccaga
      121 catgaagcca tgtcccgagt gaggatcgtt atcaaggaat aatgggtcaa gccaccaacc
      181 gttcttgctt gaatgcctgg acgaatgtat catgtggtgt cgcataaccc aaaattttcc
      241 gcgggtaatc gttcatccaa cgctggatcc gcaaaatctg gttctcgctc acctcactaa
      301 tcgggcttcc cttggggacg aaacggcgaa ttaatttgtg ctggttttcg ctcgtcccac
      361 gctcccaaga agcatagggg tggctgaaat agacgtcagc tacatcttgc agagcatcgt
      421 gtagcccaga aaactccgag ccattgtcgg atgtaacggt cttaaataat tgatcgaagt
      481 tagcgccagc ccgttctctt agctgttgaa ccgcttggtc caccgattcg gtgtccttcc
      541 cgttcaactt taggatgact tcaaagcgtg tctgccgttc agccagcgtc agcagcacgg
      601 catccgactt ggtcttgttt cccactaccg tgtcgatttc ccaatgaccg aacgtttccc
      661 gtgtttcaac ttctttaggg cgttcttcaa ttgacgtccc gagaacacgc ttgttgggac
      721 ggtgtcctgg gcggaccgct ttgattttac gggacagttt ttcaagcaag tccatgttct
      781 ttgttcgcat gattccgcgg tcaatccatt gataaagagc ggttgtgcca ggaatgatgg
      841 cacaatcgaa cagctcgtgt tttctggcaa atccgaccac gacgtccggc gaccattttt
      901 catttaacat tttgtcatct gcccaatcaa tgaaggcatt tgtgttcgcc catttggggc
      961 gtctgccaca gttcagtcgt tgcttttcat agaaggcctg tcctgcatca gcgtcataaa
     1021 cagaatagta ggtgtcatac acttttccat tttgcgtttg gcgttttaac tgggtgatgg
     1081 ttccgcgatc aatctcattg tttatggttt gcggaacacg tcccagcact ttggcaacct
     1141 gacggttgga atagccttcc tttttcagga tagcgatttg gcaacgttct gagtaggaaa
     1201 gatgttttcc cttgcgtgaa actgtgttat catgggtttg cgtcatgtga attcatcctt
     1261 gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg ttttttttat
     1321 tttaaatggc taagttcatt ataaaatccg ccaatgaaaa aatctttaaa acttgcatca
     1381 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     1441 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     1501 gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag tacgacttca
     1561 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1621 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttcg
     1681 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
     1741 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
     1801 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
     1861 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
     1921 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
     1981 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
     2041 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
     2101 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
     2161 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
     2221 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
     2281 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
     2341 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
     2401 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
     2461 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
     2521 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
     2581 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
     2641 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     2701 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     2761 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     2821 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     2881 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     2941 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     3001 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     3061 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     3121 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     3181 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     3241 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     3301 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     3361 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     3421 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     3481 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     3541 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     3601 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     3661 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     3721 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     3781 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     3841 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     3901 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     3961 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     4021 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     4081 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     4141 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     4201 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     4261 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     4321 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     4381 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     4441 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     4501 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     4561 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     4621 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     4681 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     4741 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     4801 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     4861 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     4921 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     4981 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     5041 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     5101 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     5161 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     5221 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     5281 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     5341 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     5401 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     5461 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     5521 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     5581 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     5641 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     5701 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     5761 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     5821 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     5881 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     5941 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     6001 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     6061 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     6121 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     6181 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     6241 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     6301 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     6361 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     6421 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     6481 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     6541 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     6601 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     6661 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     6721 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     6781 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     6841 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     6901 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     6961 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     7021 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     7081 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     7141 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     7201 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     7261 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     7321 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     7381 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     7441 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     7501 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     7561 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     7621 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     7681 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     7741 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     7801 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     7861 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     7921 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     7981 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     8041 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     8101 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     8161 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     8221 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     8281 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     8341 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     8401 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     8461 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     8521 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     8581 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     8641 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     8701 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     8761 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     8821 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     8881 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     8941 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     9001 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     9061 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     9121 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     9181 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     9241 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     9301 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     9361 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     9421 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     9481 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     9541 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     9601 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     9661 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     9721 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     9781 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     9841 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     9901 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     9961 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
    10021 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
    10081 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
    10141 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
    10201 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
    10261 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
    10321 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
    10381 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
    10441 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
    10501 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
    10561 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
    10621 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
    10681 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
    10741 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
    10801 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
    10861 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
    10921 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
    10981 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
    11041 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
    11101 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
    11161 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
    11221 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
    11281 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
    11341 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
    11401 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
    11461 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
    11521 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
    11581 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
    11641 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    11701 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    11761 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    11821 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    11881 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    11941 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    12001 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    12061 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    12121 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    12181 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    12241 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    12301 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    12361 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    12421 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    12481 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    12541 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    12601 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    12661 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    12721 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    12781 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    12841 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    12901 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    12961 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    13021 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    13081 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    13141 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    13201 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    13261 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    13321 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    13381 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    13441 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    13501 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    13561 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    13621 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    13681 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    13741 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    13801 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    13861 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    13921 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    13981 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    14041 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    14101 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    14161 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    14221 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    14281 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    14341 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    14401 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    14461 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    14521 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    14581 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    14641 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    14701 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    14761 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    14821 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    14881 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    14941 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    15001 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    15061 aattgccagt aagactaagg attg
//
LOCUS       DAZAOG010000062        15014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000062 DAZAOG010000000
VERSION     DAZAOG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.062"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..315)
                     /locus_tag="ACHDLD_001560"
     CDS             complement(37..315)
                     /locus_tag="ACHDLD_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363264.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            583..2592
                     /gene="metG"
                     /locus_tag="ACHDLD_001561"
     CDS             583..2592
                     /gene="metG"
                     /locus_tag="ACHDLD_001561"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN3363265.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(3071..3658)
                     /locus_tag="ACHDLD_001562"
     CDS             complement(3071..3658)
                     /locus_tag="ACHDLD_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363266.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            3833..5155
                     /locus_tag="ACHDLD_001563"
     CDS             3833..5155
                     /locus_tag="ACHDLD_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3363267.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            5310..6086
                     /locus_tag="ACHDLD_001564"
     CDS             5310..6086
                     /locus_tag="ACHDLD_001564"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN3363268.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            6079..6648
                     /gene="rnmV"
                     /locus_tag="ACHDLD_001565"
     CDS             6079..6648
                     /gene="rnmV"
                     /locus_tag="ACHDLD_001565"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN3363269.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            6641..7525
                     /gene="rsmA"
                     /locus_tag="ACHDLD_001566"
     CDS             6641..7525
                     /gene="rsmA"
                     /locus_tag="ACHDLD_001566"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN3363270.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            7976..8605
                     /locus_tag="ACHDLD_001567"
     CDS             7976..8605
                     /locus_tag="ACHDLD_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363271.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(8729..9838)
                     /locus_tag="ACHDLD_001568"
     CDS             complement(8729..9838)
                     /locus_tag="ACHDLD_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363272.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            10047..10469
                     /gene="mgsA"
                     /locus_tag="ACHDLD_001569"
     CDS             10047..10469
                     /gene="mgsA"
                     /locus_tag="ACHDLD_001569"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN3363273.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      10656..10754
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            10792..11328
                     /locus_tag="ACHDLD_001570"
     CDS             10792..11328
                     /locus_tag="ACHDLD_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN3363274.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(11368..13131)
                     /locus_tag="ACHDLD_001571"
     CDS             complement(11368..13131)
                     /locus_tag="ACHDLD_001571"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363275.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(13134..14849)
                     /locus_tag="ACHDLD_001572"
     CDS             complement(13134..14849)
                     /locus_tag="ACHDLD_001572"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363276.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         4816 a         2725 c         3046 g         4427 t
ORIGIN      
        1 aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa taaatggaag
       61 tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc gctcttcacg
      121 aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca aaatccaagc
      181 tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca tacgaatata
      241 aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg taaaaaaaag
      301 atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt tattatacca
      361 cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa caaaaacccg
      421 catttattca acctattcat ccttttaaag gatagattga acgtagatag aagtcgttta
      481 tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg tatactagca
      541 aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga aaaggaaact
      601 ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg caactcgtat
      661 actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt tgatgtgttt
      721 tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga agaactaggt
      781 gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt atggaaaacg
      841 cttgatatca gttatgataa attcattcgc acgacagatg attaccataa agcagctgtt
      901 caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga atatgaaggc
      961 tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga agtgtacaaa
     1021 gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt agaattggta
     1081 aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt ggaatactat
     1141 gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat caacaacttt
     1201 atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg gggagtacca
     1261 gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc caattatatt
     1321 actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg gcctgcagat
     1381 gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc aatcatgctg
     1441 atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct attgatgaaa
     1501 gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt
     1561 tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg gtcagatggt
     1621 gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa tgatctaggg
     1681 aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact cgttcctgac
     1741 tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc taacgttgtc
     1801 ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc tgaaatctgg
     1861 gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt attagcaaaa
     1921 gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga atctttgcgt
     1981 atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat cttcaatcaa
     2041 ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga atttccttca
     2101 ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat ggaagaagaa
     2161 gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa
     2221 tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa atttgatgta
     2281 tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt agaaggggca
     2341 gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca aatcttgtca
     2401 ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta
     2461 gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat cctttctgca
     2521 gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc aaatggctca
     2581 gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc cctttttccg
     2641 aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat catataatac
     2701 agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt cgctgatttt
     2761 tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca taatgataaa
     2821 gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct tacattcata
     2881 aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag cgttgatgtg
     2941 ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat gtgtgattga
     3001 aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc tttattttgt
     3061 aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa cggcttggta
     3121 agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc gtacgtccga
     3181 taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca
     3241 aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt gccatcactc
     3301 caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc tttgtaaatg
     3361 ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc
     3421 caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg atcaatgcaa
     3481 cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca acagtcatta
     3541 atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct ttacgggaaa
     3601 aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta aaatccataa
     3661 actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa agaaatacat
     3721 ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa attaaaatat
     3781 tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg
     3841 caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg gaaccgtgat
     3901 cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt ctacaagttt
     3961 gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat tgacagtggc
     4021 tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg aagctgcgta
     4081 tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact ttcctgctaa
     4141 tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac aattatctac
     4201 cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt tgaatttgtt
     4261 agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat taataccaat
     4321 tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc agttatttcc
     4381 aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt tgttagcaac
     4441 gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc
     4501 ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag tcgtttatct
     4561 attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg gtaatttgaa
     4621 tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc tggtcactat
     4681 tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg ggatccgcgt
     4741 gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga caaagctttc
     4801 aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag cttgtattat
     4861 gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga tgtggctatt
     4921 cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg aattgccacg
     4981 tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg gtgctatttt
     5041 cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta taggtgttac
     5101 cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat
     5161 ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc tttttctatt
     5221 tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt gcttatgtgc
     5281 taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc catttaaatg
     5341 ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa atgggggtaa
     5401 cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg caattaagcc
     5461 atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct ggcagttata
     5521 cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt gtagcattag
     5581 gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa gataaagtat
     5641 ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt catactagag
     5701 aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat atcggtggaa
     5761 tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac ttagggatgc
     5821 atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa gaggctgcag
     5881 caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg gcaccagttc
     5941 cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa aagattgctg
     6001 aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat
     6061 tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt cgttgaagga
     6121 aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga aacaataggg
     6181 tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac tcgtggagtg
     6241 atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat tatggaagct
     6301 gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga
     6361 agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa aaaggtcgtg
     6421 acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct tttagactat
     6481 ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc
     6541 atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg tattacgccg
     6601 aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat
     6661 tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat ttaaaaaaag
     6721 tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca
     6781 aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat tgacggaaca
     6841 attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt tgatccctgt
     6901 attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag atattttaaa
     6961 gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt taaaagtcgt
     7021 agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg aatcagattt
     7081 ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta tctctgctga
     7141 accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata tggaagccag
     7201 tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag attcagcgat
     7261 tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa aagcattttt
     7321 ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata acttgcagca
     7381 ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg aaacagctgg
     7441 aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg cattaagcaa
     7501 tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg
     7561 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt
     7621 tgggcttgtt accgcagatg caagcttttg aacaccgttt aagaaagagg ttgtgggcct
     7681 gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat gtttttgagt
     7741 tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac aaggagatgt
     7801 gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc ttatacttga
     7861 tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag ttattagtaa
     7921 gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag aaaaaatgag
     7981 aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg gggtcattat
     8041 cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg cgtctcatgt
     8101 gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg cgctaggtac
     8161 gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat tgtcacatct
     8221 tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa
     8281 tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga tcggtgtgat
     8341 tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta atatgcctga
     8401 aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga ttggtgggct
     8461 tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac tcagcaaata
     8521 ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg
     8581 gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc tcatctagta
     8641 atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg caccaacatc
     8701 ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt tctgtttctt
     8761 tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg cctggtttat
     8821 ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt gtgtaaagca
     8881 tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg gcttcaggtg
     8941 aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg aagataactt
     9001 cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga atacgaagct
     9061 taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag aagttcatgg
     9121 caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa acttctttcg
     9181 gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca gccatagtca
     9241 ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt
     9301 tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat aatggctcat
     9361 ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt
     9421 gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct aagatctctg
     9481 ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat ttcaagccaa
     9541 aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg aaaaccatgg
     9601 caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata tttagttctc
     9661 cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg
     9721 aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa tcagttacag
     9781 aataattttc tgcattgtca tattttttat atacgttttt cagagccatt tctaccataa
     9841 tacgttccac actttctttt ttagttttat taactattta aagtataaat gaaagcgtta
     9901 actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat gtgctagttt
     9961 taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa ataagtatgt
    10021 taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa
    10081 aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa catgaacttt
    10141 ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg atccatcggt
    10201 ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg gaggatcagt
    10261 tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa ccagatgtaa
    10321 cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat atcggaacag
    10381 ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga atcgtccatg
    10441 aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat aacgtttaat
    10501 gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata taatacgaac
    10561 gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat gtgaaaatat
    10621 gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat aaagcgttaa
    10681 gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc ggttttattt
    10741 tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa
    10801 caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt gatggtcact
    10861 ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac atttattcca
    10921 caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc agttttagct
    10981 gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt tacattgaat
    11041 gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat cacttggaaa
    11101 aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt gacaccatta
    11161 tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc gccccgcctg
    11221 ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt aggacgtagt
    11281 attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa aacaagcagt
    11341 cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt gatagagccg
    11401 tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga caatagctcc
    11461 ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat gggcaatcac
    11521 aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt tctctgtcaa
    11581 tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac tgatcatcgt
    11641 tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt gaccgccaat
    11701 cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag aggcttttag
    11761 ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat tttctcgcaa
    11821 tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac gtaatgtatc
    11881 acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg ttgtgtcata
    11941 aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc ctactatagc
    12001 aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc
    12061 ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg gttccaaggc
    12121 ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa atgctcgttc
    12181 tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga taaatggttt
    12241 agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc
    12301 actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa ggtgatctac
    12361 aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc ctttttcata
    12421 tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct gaaaagcttt
    12481 cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc caataatcct
    12541 ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca caaggaaaat
    12601 aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca tcataaaaat
    12661 aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg cagaaattgc
    12721 aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt gggagttttg
    12781 atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc ttaattttgc
    12841 aacggataag taagctaacc gattgcttaa cacttggatg agccattgac tgactacgtt
    12901 gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga agcggacctg
    12961 tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca caactgttgc
    13021 aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat aggcagtaag
    13081 gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca taactattct
    13141 tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg
    13201 gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc ttcttgaata
    13261 gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg tagcgcctta
    13321 cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa aatcagtaat
    13381 gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa
    13441 tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg gacaaattct
    13501 tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc tttcttaccc
    13561 cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg aacaagagca
    13621 atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc cattataact
    13681 gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt
    13741 ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt aatattttgg
    13801 agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc gataattgct
    13861 tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct
    13921 agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac gacgaggttt
    13981 gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc aagaacttgt
    14041 ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa acagatgatc
    14101 ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt ggaaacacgt
    14161 aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc tttttttgtc
    14221 tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg ttcattgagc
    14281 tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat gataaaaaat
    14341 aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc ccagtcgatc
    14401 acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa taaacgaatg
    14461 accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt gatcaaggta
    14521 tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt gattagctga
    14581 ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg gcaaatgata
    14641 gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc aatgattttt
    14701 cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa aggcaggaca
    14761 agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg cttacgatag
    14821 tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat ttgtccacaa
    14881 ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa agagaacaag
    14941 ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata gactattttt
    15001 tttaaaaaaa agaa
//
LOCUS       DAZAOG010000063        14815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000063 DAZAOG010000000
VERSION     DAZAOG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.063"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHDLD_001573"
     CDS             complement(296..1024)
                     /locus_tag="ACHDLD_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363277.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHDLD_001574"
     CDS             complement(1002..2576)
                     /locus_tag="ACHDLD_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3363278.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHDLD_001575"
     CDS             2805..3743
                     /locus_tag="ACHDLD_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363279.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHDLD_001576"
     CDS             3755..4666
                     /locus_tag="ACHDLD_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3363280.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHDLD_001577"
     CDS             4690..6162
                     /locus_tag="ACHDLD_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3363281.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHDLD_001578"
     CDS             6213..6824
                     /locus_tag="ACHDLD_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3363282.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHDLD_001579"
     CDS             6854..8767
                     /locus_tag="ACHDLD_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN3363283.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHDLD_001580"
     CDS             8895..11876
                     /locus_tag="ACHDLD_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN3363284.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHDLD_001581"
     CDS             11873..13534
                     /locus_tag="ACHDLD_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN3363285.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHDLD_001582"
     CDS             13574..14506
                     /locus_tag="ACHDLD_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN3363286.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZAOG010000064        14344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000064 DAZAOG010000000
VERSION     DAZAOG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.064"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACHDLD_001583"
     CDS             <1..83
                     /locus_tag="ACHDLD_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFN3363287.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            110..445
                     /locus_tag="ACHDLD_001584"
     CDS             110..445
                     /locus_tag="ACHDLD_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363288.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            622..1572
                     /locus_tag="ACHDLD_001585"
     CDS             622..1572
                     /locus_tag="ACHDLD_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3363289.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2500
                     /locus_tag="ACHDLD_001586"
                     /pseudo
     CDS             2090..2500
                     /locus_tag="ACHDLD_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2943..3755
                     /locus_tag="ACHDLD_001587"
                     /pseudo
     CDS             2943..3755
                     /locus_tag="ACHDLD_001587"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3750..3914
                     /locus_tag="ACHDLD_001588"
     CDS             3750..3914
                     /locus_tag="ACHDLD_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363290.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3854..3976
                     /locus_tag="ACHDLD_001589"
                     /pseudo
     CDS             3854..3976
                     /locus_tag="ACHDLD_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4538..5044)
                     /locus_tag="ACHDLD_001590"
     CDS             complement(4538..5044)
                     /locus_tag="ACHDLD_001590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3363291.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5049..5816)
                     /locus_tag="ACHDLD_001591"
     CDS             complement(5049..5816)
                     /locus_tag="ACHDLD_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3363292.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="ACHDLD_001592"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="ACHDLD_001592"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFN3363293.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="ACHDLD_001593"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="ACHDLD_001593"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFN3363294.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="ACHDLD_001594"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="ACHDLD_001594"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFN3363295.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="ACHDLD_001595"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="ACHDLD_001595"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN3363296.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="ACHDLD_001596"
     CDS             9217..9534
                     /locus_tag="ACHDLD_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3363297.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="ACHDLD_001597"
     CDS             9534..11240
                     /locus_tag="ACHDLD_001597"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN3363298.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="ACHDLD_001598"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="ACHDLD_001598"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN3363299.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="ACHDLD_001599"
     CDS             12811..13083
                     /locus_tag="ACHDLD_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFN3363300.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="ACHDLD_001600"
     CDS             13104..14003
                     /locus_tag="ACHDLD_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN3363301.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DAZAOG010000065        14103 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000065 DAZAOG010000000
VERSION     DAZAOG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.065"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1049
                     /locus_tag="ACHDLD_001601"
     CDS             <1..1049
                     /locus_tag="ACHDLD_001601"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFN3363302.1"
                     /translation="IIIAICVLTVFLPQITEYLNYRIFTTKVDDSVTSLSRLEFIKIC
                     ASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKELLTQIDREFYFLL
                     NMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEIGSTIEQSGPLKG
                     TVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQIYLGRYFKMFHR
                     IVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYIKRGFGLGIQLIG
                     SGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEYKKIA
                     NFESLYKLFNETKL"
     gene            1093..2265
                     /locus_tag="ACHDLD_001602"
     CDS             1093..2265
                     /locus_tag="ACHDLD_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3363303.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            2252..2956
                     /locus_tag="ACHDLD_001603"
     CDS             2252..2956
                     /locus_tag="ACHDLD_001603"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN3363304.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2961..4121
                     /locus_tag="ACHDLD_001604"
     CDS             2961..4121
                     /locus_tag="ACHDLD_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3363305.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            4197..4475
                     /locus_tag="ACHDLD_001605"
     CDS             4197..4475
                     /locus_tag="ACHDLD_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFN3363306.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            4456..4827
                     /locus_tag="ACHDLD_001606"
     CDS             4456..4827
                     /locus_tag="ACHDLD_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFN3363307.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4843..5247
                     /locus_tag="ACHDLD_001607"
     CDS             4843..5247
                     /locus_tag="ACHDLD_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363308.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            5244..5654
                     /locus_tag="ACHDLD_001608"
     CDS             5244..5654
                     /locus_tag="ACHDLD_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363309.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            5715..6302
                     /locus_tag="ACHDLD_001609"
     CDS             5715..6302
                     /locus_tag="ACHDLD_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3363310.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            6375..6842
                     /locus_tag="ACHDLD_001610"
     CDS             6375..6842
                     /locus_tag="ACHDLD_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFN3363311.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6888..7196
                     /locus_tag="ACHDLD_001611"
     CDS             6888..7196
                     /locus_tag="ACHDLD_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363312.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            7391..>14103
                     /locus_tag="ACHDLD_001612"
     CDS             7391..>14103
                     /locus_tag="ACHDLD_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN3363313.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQ"
BASE COUNT         5047 a         2185 c         3042 g         3829 t
ORIGIN      
        1 ttataataat agcaatttgt gttttgacag tatttttacc acaaattact gagtatttaa
       61 attatcggat atttaccaca aaagtagatg atagcgttac tagtttaagt aggctagaat
      121 ttataaaaat atgtgcatct gtgtttggtc ctattttgac tgtaatggta tttgtgaata
      181 ctttaaatat gcagaaaaaa acagagcaaa agcaaaatga gcttgaagta aagcaagcta
      241 aggctgagaa cgagagagca aaaaaagagc tgttaacaca gatagatcgt gagttctatt
      301 ttttattaaa tatgtttatt gaaatacaaa aagatcaaaa tgccaaacaa gctgttattg
      361 ctttacagaa gttggcaatt tttaacgaaa agggttttaa tcaagactat gcatttactt
      421 ctaaagattt atcagatagt gaaattggga gtacaattga acaatcagga ccgctgaagg
      481 gcacagttga atatataaaa tttgaaacat ttgatgtaag caagtcgatg gggaattatc
      541 aaacaaaaac aaaagacgtt gctacaagtt tacacgatca gaaagtagct atattgaatg
      601 aacaatatga aaagatgcaa atatatcttg gacggtactt caaaatgttt caccgaatag
      661 ttaaaacatt aaatgagtat tatgacgatt ataatgattt tgatgtgaag aggtatacaa
      721 aatatatagg gacattaaga acacaaattt ctccagctga attccaagtg attttattca
      781 attctctata tataaaacga ggttttggct taggaattca acttattgga agtggttttt
      841 ttggagatga ttttgatttt gaaactaatc aacattttga aacaagtatc aatgaacagt
      901 ggtttctatc actatcaact gttgacagtg ataactctat aaagcgacaa aaattatctg
      961 aatatattaa agaattagga tccgtagaat ataaaaaaat agctaatttt gaaagtttat
     1021 ataaactctt taatgaaact aagctctaga aatagggctt ttttattttt ctttgaaagg
     1081 tggtgaaaac atgtgagctt atttgatatc tttaagcagt caataagaaa tgaagagcct
     1141 tctgagtgga taccggattt tgtatatgga gaggaagtat ctgctcgggc atacctcaaa
     1201 attatggcaa aaaatactgt tttagatttt gttgcaagaa ctatgtcaac gttagaagtt
     1261 aagtttaaaa ataaagatgg gacatcagac tgggattata ttctaaatgt tcgacctaat
     1321 tctgatatgt cagcagctac attttggcaa aaattttttt atcggctctt agatgacaac
     1381 gaagtgttag taatatttac agatgataat caattgctga ttgctgatga ttttagtcga
     1441 acggagtatg ccgtgtatga tgatgttttt acaaatgtga tggtaaaaaa ttttgtcttt
     1501 gaacgttcat ggaacatgtc tgacgtcatt tatatggaat ataataacga agaattagaa
     1561 aggtttacac gaggtttatt tgaagattac gccgcgttgt tcggacggat tctagaagtt
     1621 gcaatgcgta acaaccaaat tcgtgggtct gtatccattg attcaacggg aacggcgaat
     1681 gatgaaaaag gaaaagatgg taagactaga tctcaaagat tacaagaata tattgatagg
     1741 atttggaact cttttaaaac taactcagtt gcaattgttc ccaaagtgaa aggctttgag
     1801 tatgaggaat acaccaataa acaaggcgta tcgaatcaat cgctagatga actgaataag
     1861 atgaagtcgt ctttaattga tgatgttgcc aacgccatag gagtacctac ggcgcttatt
     1921 tatggtgaaa aagctgaact ggattcaaat attaaagctt ttcgtaaatt gtgtattgct
     1981 ccactagtta aaaaacttca agacgaattg gtggcaaaaa taattagtcg tcaagagtac
     2041 aaaaacgggg aacgtataga agttaaaaaa gttctccaaa aagatctatt agagtatgct
     2101 actcaaattg ataaaataac atctggtggt gcgttttata ttgacgaagt tcgtgatgaa
     2161 gttgattatg atgagttgcc aaataatgaa ggaaaaattc gtattcgtac gaaaaactat
     2221 gaagaaactg tgaagggagg tgagaacgac aatgccgaaa gctaaaaaag taccatttca
     2281 gtttactaac gaaattcaga atggtaagca cattctcacc ttgagtggaa atgtacagaa
     2341 aaaatattgg cgtgatgatg atgtcataaa cgctaaagat attcgtgact ctctagatac
     2401 tgtaaccgat gatattgtaa tcaagttgaa cagcccaggc ggtgatgtgt ttgaagggat
     2461 tgagatttac aattatttaa aagatcaccc atcaaatatt actgtagaag ttacagggct
     2521 tgccgcaagc gcagcaacgt ttattgtcgc tggtgccaat gatgtgatta tgaatgttgg
     2581 gacttctttg atgattcatg aagcttctac atttgcttgg gggaataaac aggacatcca
     2641 aaaaacgtta aacgcattgg aaacgatcga tgattctatc ttagctattt attcagataa
     2701 gactggccaa tctgttgata agttgcgtga atggatgaat gaagaaaaat ggtttacagc
     2761 agatgaagct gtcaaatatg gatttgccaa ttctgtgaag cgtgaagaac cacaaaatga
     2821 acctcaaaat attgcagcga tgattcaaga tgctgttgcc gttgcaatag cgaatttaaa
     2881 ggaacctgtt actaataaaa tcgaacaaga atcaaaacaa aaatcattaa tcgcacgatt
     2941 gcgaaaagga gaataaagat atgttaaaaa ttacagataa aactgcagat gcaaaaaagg
     3001 catttaatgc tatttcttcg aaagaagatg caacacccga acaaattaat gctgctctag
     3061 aagaatatgt aaccgccatt gcagaagatg ccggcaaaca ggtgcgtgct gaatatgaag
     3121 aattgaaaaa tgtgacggac aaccgtgtat tagaggctcg tggaattcct acactaacag
     3181 cagaagaaac aaagttttat aatgaagccg taaaatctgg aggtttcgat tctgacgtag
     3241 tttggccaga aacgattttg gaacgtgttt ttgaaaatct tcaaaacgat catcctattt
     3301 taggcctcat taactttaca ccaactgtag gtcgtgtcaa agtaattaga gcacgtcgta
     3361 aaggtgttgc tgtttttgga ccattgcata aagaccttga aggccaacta gatgctgaat
     3421 ttggtgcaac tgaatttgtt caacttgcgt tgaccgcatt tttcttaatt tctaatgata
     3481 ccttagattt aggaccacga tggattgacc gttttatcaa tctttcttta agtgaagcag
     3541 tacgtgatgt ttgggctaaa aaaattatta caggttctgg taaagatgag ccaattggat
     3601 tgcttaaaga tctagatggt gcggtcacaa gtggtgtata tccagataaa gctagcgcag
     3661 ggacattaac ttttgcgaaa gagaagattg ttcttgaact tgcaggggtg atgaaaaaat
     3721 tatctaaata cacatacaaa gtagatggtg acgatgaagg tgaagtgaaa taccgttctg
     3781 ttgctggacg agtgtatcta attgttaatc ctgttgatta ctgggatatc gtagctagag
     3841 ttacatttgc aaatttaaac aatgtgtatg ggactacgat gccatttatc gctgttgatc
     3901 atatcatcga atctgtagac gtaccagaaa gtaaatgtat tgcatacgtt gataaagagt
     3961 atgaagctac tcaatcaaga gctgaaaaag tctatgtata taaagaaact tttgcaatga
     4021 aacgcgcaac tttgtatgct gtcgacatgc ttggtaatgg ctatccaaca aataatgatg
     4081 cagcacaagt atatgacttg gactttacag cgggggagta atgtcccctg caattggtga
     4141 gataacaccg acgactgatg gggcagtcat taatctttcg taaatggggt tagagtatga
     4201 acactgaaga atttataaaa gaatataagg cccgatttcg tattttccat acttccgaag
     4261 atgaggatat cgggaaacag ttagagagcg ggtttgccga tataaaatct ctaatcggca
     4321 gttttgatcc ttccacttat gagaaaggaa aagaattagt ttatgaacgt actcggtatc
     4381 tacggaacga atcactagag tacttttacg acaattttca aacaatgatt atggatgcat
     4441 caattgatct ggcaggtgct ggatatgcca attaaatcaa attataagaa gcctaaaatt
     4501 gtagctggtg atctaaatac gccagttact ttttttgagt ttcaacctaa taagggtcca
     4561 gaacctggtg atgaagaaaa aaaggaactg tatttttgta cagcactagt ttacaaccca
     4621 tcaatgaaag atcgtgacat tcttggcaca aaagaaggcg ttacgataaa aatacgggat
     4681 ccccatcaat cttatattcc tactaacaaa cataaagtaa ggatattaga ttatcgatat
     4741 ggagataaag tttgggaaat aatcgatgtc tcaccggatt tggaagaaaa ttcaattgta
     4801 aagatagtat tagaattaat tacttaaatt tggtgataaa tgatgagtaa cgtaaagttt
     4861 gaaggcgttc aagaaacaat ccaagcttta gaaaaaaagt atggagaaaa gaaaactaag
     4921 acattaacaa aaaaagcaat caatgttggc gctgaaaaag tagaaaaaca gttgcaaacg
     4981 gacatgaccg tttttaaaga caaaggttat acgatcgctg aggttgttcg caagaacgct
     5041 acgtatagag attacaaggc agaagctgaa atcggttgga atggtccaca tcaacgatat
     5101 agaatcattc atttgaatga atggggatat acacgaaagg gcaaacaaat tagaccgaga
     5161 ggatttggtg taataacaaa atctcttaag aattccgagc tgttgtactt tgatgctgta
     5221 ggagaggagg ttaaaaagag tttatgaaag atatgcttaa tatcatttac gaggctcttt
     5281 gtttgaatga gtatattcat tcaatgacct acaacgagaa taccgagcaa tatcgtatta
     5341 aattttatga gcaacctgaa accgccgata aaacaggtcc aatgatcaca attcgtcctg
     5401 tagatacacc taacgaagcg taccacggaa gtaacaaaga gctttctgtg gagcatatga
     5461 tacagattga cgttgaatcg aaatatagag caacatgcaa gcagattcaa tatgagataa
     5521 aaaaagagat gaaaaaacta ggctttggcc aaatttccgg tcaagggtta gatgaatact
     5581 ttactgaaac aaaccgcttt gtagacgcgc gtcgttacga aggaaataca aaaatttacg
     5641 ataccgaata ttagaaatta agacacgaaa ataacgtgtc tttttttatt acccaaaatt
     5701 aggaggaaaa ttttatgact ttagtaggat tcaaaaaaat gacaatcggg gtctttgaca
     5761 gcacagggaa gattccagca gcaaatttat atgtaattga aggtgaacaa aacaaagggg
     5821 cgactgtatc tgctgaaatc agtgggctat caaaagaacc atcaaaagtt tacggatcga
     5881 acatcgctta ctatgtctct caaaaaggaa caggcgatgt ttcagctact tttggattgt
     5941 tagatttacc aacagaagta aatgacaaaa ttcttggtta caaaaccgat acaaacaaaa
     6001 tcagcttctt gggagaagat actgagccac catattgtgc aattttgatg gaatcggagg
     6061 atttgaacgg cgatacagca atgcttgcga tgttcaaagg aaaattcagt cgtgaatcta
     6121 ttaatcttaa cactacgaca aatgaagctt tcgaaccaga agcagaagaa tatgtatttt
     6181 cagcgattgc caatgatgtt gaaggtgatg ctaaaggcca aacagtcgca aaatatgttg
     6241 gagacgaaga agcttctatt actggtttga aagagatggt atttcctgca gcgggggagt
     6301 aactagcccc gttgttggag caatcactcc cactattaat ggggcaacag ttgaattaag
     6361 ttaggaggat aactatgcca gatacattta aaatctataa aaaaaatggt actgaattta
     6421 caaaagtcgc agagggtgaa agtccacttt ctattactgg aattgcagca aatactcaag
     6481 ttgctaaagg agattatcaa gctacgcgtg tggtcggagg tcaagaatca gtaaaagtag
     6541 atattcctgc attcaccact cttccaattt cagttactgg tgtgacgttg gataaaacga
     6601 cagctgaagt tgaacaaggc ggaaccttaa aattaactcc tactgtaaca ccagcaaatg
     6661 caacagacaa aacaggaagc tggagcagtt cgaatacagc tgtagctacg gttactggag
     6721 gcaacgttac agtaaaaaca gacgctgaag ttggcggaac tacagaagtt tcatttacta
     6781 ctacggatgg cggtaaagta gcaaaatgta ctattacagt tattgaaaaa gcagcgggct
     6841 agtcttcgga ctagcttttt tatttggaaa ataatggagg aatctaaatg gcaaaagtaa
     6901 gaattgaatt gaaagaagaa tcaggaaaga aagtctatga aaacatagac acaacaggga
     6961 aagattatcg aaaagcttta gaaacaatca aaaagctaaa tgaagacgga gtaatgattt
     7021 ggaatcgttt agacatttat ttggattttg ctgtaggtat cttcagggat agcaaattga
     7081 cagctgaaca aattcttgat ggcttaccat ctgagaaggt aatgagcaca ttggacgaca
     7141 tcctaggaga agtaatgggg attgaaagta atccagaccc agacgcaaaa aagtaacacc
     7201 cgaagaagca gaagaaatgt acttaaacct atgcagagag ttagtgaaag cgggatggtc
     7261 actatctgac atagaagata attcctttga aatgatgatg aaaattgcgt gcacttcgcc
     7321 taagaaagag aaacaaaaag aagttgatct taaagacttc ttgaaatcca tgtagagagg
     7381 aggtaaacat atggcaaatg gaaaaccttt aggcaatatg attatcaaac tagacttaga
     7441 cagttcagct ttttcaaaag gtctagccgg agctaaaaac gctgttaacc atcaaatgaa
     7501 agcaatgaaa tcacagatgc aagtaatgaa ctcttcagga aattctctcg gtgctttaca
     7561 agcaaaatac aatggtttag gcggagttct acaagcaaat gaaaagcaag tagaattgct
     7621 cactaaggct tataaggata gttttgactc taacggaaac gctactgctt cgactgctaa
     7681 atacgctaat caattgaatc aagcaacagc tagatcagca agttacgaat ctcagatgaa
     7741 aacaactgtt ggacagattg ctcggatgaa agtggaaacg gaagggttta cggggaaact
     7801 aaaggctaat tctgatgtat tgattaattc tgggaaaaaa attgaagcct ttggtaaagg
     7861 agtttctggg ataggatctg ccctaactgt tggagtaaca gctccaatag tggcaggcgc
     7921 aactgctgtt actagtgcag ctatttcatg ggaaagtgct tttgcaggtg taaaaaagac
     7981 aaatgatgaa gttgtagact cgaatggaaa cgtagtttat tcctatagcg acttagagaa
     8041 aggtctgcgt aatcttgcca cccaattacc tggtagccat caagaaatag caaatgtagc
     8101 cgaagcagcc gggcaattag gtataaagac tgaaaacgta gttagcttca ctaaaacgat
     8161 gatagacatg ggtgaatcga ctaatatgtc ggctgaaact gcggcaactt ctttagcacg
     8221 atttgctaat attacccaaa tgtctcaaaa agactttgat aaattaggat cagtcattgt
     8281 tgatctaggt aacaattttg ctacaaccga atcagaaatc actgaaatgg gattgcggtt
     8341 agcgggagct ggtaaacaga ttggaatgag ccaaggcgaa attctaggat ttgctactgc
     8401 tctatcttct gttggtgtcg aagctgaagc gggtgggtct gccttttcga aggtcatgat
     8461 ccagatgcaa ctagctgtag aaaaaggatt tggcgctttt gatcagctga aacagatggc
     8521 agaagaacaa ggtgttccgt gggttaattt agtaaatgct gtgagagacg gtggaaaatc
     8581 gttaaaagca gtatcagagc aaatgggatt cacttcatct gatttaaaga aaatgtataa
     8641 agaagcggac aattcgaaaa gttcgcttga aaattttgcg aatgtggcag gtgttacaag
     8701 tgaacaattc cagaagttgt tcaagagtga tccttcagaa gcaatcatca agttcattca
     8761 agggctgaaa gattcagaaa aacatggtac ttcagcaatc aaagttctgg atgacatgga
     8821 tataaaagaa gttcgactac gcgacagttt attgagagct gcaaatgcta gtgacatatt
     8881 caaaggagca attgaaaaag gaaacggcgc gtggaaacaa aatacagctt taacaaatga
     8941 agctaataaa agatacgaaa caactgaatc aaagctaaag atattgaaaa acgaagtgat
     9001 tgatacagca atcgatcttg gaggtccatt tgtagacgcg ttgcgtgatg gtctaaatgt
     9061 atcgaaacca ttgataaaaa acttagggaa tatggcgaaa gctttctcag ctatgagtga
     9121 tgatcaacaa agaaacatta tcaaatgggt tggattagca gcagcagctg gtccagcgct
     9181 gaagctttta ggaactggta cttcagtaat aggtaaagtt acttcaacta ctggaagatt
     9241 gacgaaaagc cttgttgatt tagcagcgaa agcagcagaa aagaaggcta tggatgcctt
     9301 tgcatcgtcg gtaacaacag ctggtgctgc agctgcaaag acagcaggag ctggaggtat
     9361 tggcgggtta gtccctatga tgggaaaaac agctagtgct gcaagtactg ctgcgggcgc
     9421 cagcggaata ggcgcaatga caacgtcact aggtttgtta ggtcctgctc tacttggtat
     9481 tgttggcgtt ggtggcgcac tggcagttgg ctatggagca tggaaattat ttggtgaaga
     9541 agcttggaat tcctcgcaaa gagtaaagca atggggaaca gatgtcggtg aggagataga
     9601 tagtactctt gacggagtac agaagaatat tgaaggtgca aatggtcaat tcggtcttct
     9661 caaagagggt tttactgttg atgatgcaaa taatatggcg gctaatttcg caaccgctgg
     9721 aacatctttg gaaacatctt taacgggtag aataaaggca ttagacgatg cactaaaagg
     9781 gctacctcaa tcagttcagg atgccatgaa agaagtagct gaatcagaag aagaaacctt
     9841 agggcaatct ctttctaaaa taaaagacaa caatgaagaa ataaaaaaaa tcagagaaag
     9901 agccgcaaat cagaacagag acttgactgt ttcagaacta caacagatac aggatctaat
     9961 gaaaggaact tctcaagcgt atgtggatac gctcgatgtt tctgctaaag acagaaaaaa
    10021 gatactatca gccatgaatg gtgatgttgc acaagctaca gaagaagaag cgaagacgtg
    10081 gttacaatct ttagcgaaac aaaaagctgc aactaccgaa aattatagac aaatgcttga
    10141 agaaaaaaag gcggctttaa aggaaaaggg agtaagcaaa gaattcattg atgcattaga
    10201 agatgaaatg aatgattaca tcaaaactac aaatcaagga ttcgatagcc aaatagctgc
    10261 tataacaaat aaatatccac aattgattaa cgaagtgagt ttatcaaatg gtcaattatt
    10321 tagcaaaacg gcagctgcta tggacaatac tggtcagtat acaaaagttt ttctcgaaag
    10381 taatgaaaag attttagccc aagcgaagaa aacatcggat gaaatagcag ataatgccga
    10441 aaaaaatgca gaacaacttg gtatggtagc tgatgaggct acgcatgctg gatcaacttg
    10501 gaatggctta gttttagacg aaaaaacagg aaaagtaaaa actaatgtac aagaagtagt
    10561 gaatgaagca acgaaggatt tcactaaatg gaatgaattg aaacctgttg ttcatgatgc
    10621 gaaactaaca acgaatgcaa aagatgtcat ttccatagcg gcagtacaaa atggctattg
    10681 ggatgatatg gaatggaaag aaaaagaagc attattagaa gatgagtgtt ctaaaaatgt
    10741 atacaaggct ttagaaaatg ctggtaaatg gaatgagtta gatattcctt ctaaagaagc
    10801 aatcctaact tcaaatactc ctgaagttat gggtgaaact ctcatgaatt tgggattatg
    10861 ggatacttac aaacttcaag tgaaagattt agatttgaat gcgtataaag tatacgatgc
    10921 tatcgatgga tcagaagaaa aattaaatag ttgggctcaa ttagataacc ccacaaaaga
    10981 attgttatta gataactcag atttttctac aaaacttttt aattccgaag aaatgtttaa
    11041 acgttggaat cagttatcat ctaatgcaaa gtttctttcg gctaataata ctaaatttac
    11101 agaggttctt ttatcatctg acagaatgtg gaatcaatgg gtaaaccttc ctgataatga
    11161 gaaaagatta cttgcagata acgaagattt agctaccact gttttatctt cagaagaaat
    11221 atacggaaga tggactcaat taccagataa tcaaaaatat atgttagctg acaatactga
    11281 cttagcaaac aaaatattcg cttcagaaga atcatggaat gcatggaaag caattccaga
    11341 cacagttaaa aacttgcttg gtaataatat tgatttactg gctaaagtga aagacgggac
    11401 tgtaagtgta gaagaatata acaaagttat gccacttctt aaaactctat atggagataa
    11461 cgttaattta aaagaggcaa tcagtggggc taaaagctct atagagaatt acaacaaaaa
    11521 tatttttcct agtgataaaa ctgcagtagg acacgatgag gcttcacaag ctgctaaaga
    11581 tgcatttgaa gcgttcaaca tcttccaaac aaaaatacca gataaaatta ctaaaactgt
    11641 atcagctgac tttataggtc ctatgccgaa aaatgcgaaa ggaacaaact tccatcctgg
    11701 cggagcagct atggtaaacg atcaaaaagg gcctacttat gaagagctta taaccttgcc
    11761 aagcggagaa acttttatac ctaaaggacg taatgtcgtt ttagacttac caagaggttc
    11821 gaaagtactg aacgccacta aaactaaacg tctagtgcct aaatatgctg atggtatagg
    11881 aaacataacg acggtttcat ctacgctaaa cttagatgct ttaattttag ctattaacga
    11941 gctaacgacg gtattgagaa ctcaactgat ggcaggaacg actgatgcta aagatactaa
    12001 actatcagat gaaacaaaag ttgccgatcc gattattccg gacagtcttt ctgaaaaatc
    12061 agatcagtat ctaggaattg gagctcaatg gttaactaat ctaatgaatg gttggaactc
    12121 agcggttcct caatatatga atagtgaaat ggtttttatt acaaactatc tcaatgcgct
    12181 aaaacttcaa aacaatccta attatacgca aggagcaaca tggaataaga acttactgaa
    12241 tggatggaat agtttaactg ggacctttat tgctacaatc aattcatttt gtaatcaagc
    12301 aatggtcacg cttagaaact acaacactcc tatgtacaac aatggccgaa cgtggcagca
    12361 aaataatctt aatggttgga attcattgta tggatcattc atagcccgtg taaatcagct
    12421 tggtaacgat tcgattaaca accttcgttc taaaagtggt ggtttttaca atgcagggac
    12481 attcttgttg caatctttaa taaacggaat gaattcgtta ggtaattctc tttctactac
    12541 aatgaacaat gtcgctaata caatggttgg tggtataggc aaaggagtta atggcgtcat
    12601 atcgggagtt aactatgtgc ttaaggaagt cgaatcaagt aagaatatcg gaaactggcc
    12661 aattccacaa tacgctaagg gaactgatgg acatcctggt ggacttgcta tgattaatga
    12721 tcaaaaaggc ctagttcacg aagaatatgt tcagatgcca gatggacgta gttttattgc
    12781 caaaggtaga gatcttcttg ttaatttgcc taaaggtgct caggtcttaa atgcttcttt
    12841 aacaaagaaa ttaaaggaac gtttaaatat tccacactac gaaaaaggta ttggcaattt
    12901 agatattgtt gatttgttag atgatgaaaa aagaatgtta gaattcttaa ctagcaaagt
    12961 tgatttttca ggcattaatg aaccatggct tgatatgact aaatcaggaa cgtcattaat
    13021 gtccaaagct gcaaatacaa tgcttcaatc aaaattaagt gaattcttca ctcatggcaa
    13081 ttttgatggt gcagtaaatg ccaatggtgt ttatcaatat ttagttgatg tggcacagaa
    13141 agtaatgggt aagttccccg gacttacagt aacttctgga tatcgagcag gagatgccta
    13201 ctatcacgga aaacggcaag ctatcgactt ggcttatcca ggtatttctg gagatccgag
    13261 atatacagca gcagcaaatt acgcttttga aaaattccct tcaaaaattg cgtatgtcat
    13321 tacgaatgga cgtgtacgtg accgcatggg attatctgga acaaggtcaa gcggacaatg
    13381 gacgaattgg ccagatggcg atcacttcga tcatattcat ttaaacggtt cgatggggtc
    13441 tggcgatata ttcaatggag gagttggagg aagtggtgtt gcacgctggc gttcttatgt
    13501 gtctaaagca ttgaaaatga atggattacc agctacagca gcgtatataa atgcctggat
    13561 gtcccaaatc caaacggaat ctggtggtaa tgagaaagca atcggcggca atgatgggct
    13621 cgccgaaggc aatgcaacag gattacttca aacgaaaccc ggaacttttg cagctaacgc
    13681 ttttcctggt cacggaaata ttatgaacgg atttgataac atgttagcag caatcaatta
    13741 tgctaagaaa cgttacggag ttgcaggtat gttgcaagtt atcgggaaag gacatggata
    13801 tgccaatggc ggcttgatta ccaaagacgg actatatcga gcaggagaag gaaataaacc
    13861 agaaatggtt attccattga caagaaaaac aagagctatc gaacttatgg gtcaagcatt
    13921 agccttcctt tctggaagca ataaaaaaga aacgaaaagt gagattaatt ctgtagataa
    13981 tagacatatc ctacaagcga tagaaaaaca gtctcgtatg acaaatgatc tgctatcatt
    14041 attactctcg ttttttaaaa gtaacaatcg atcagataaa gaactagctt tagatattca
    14101 gaa
//
LOCUS       DAZAOG010000066        13918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000066 DAZAOG010000000
VERSION     DAZAOG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.066"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHDLD_001613"
     CDS             99..746
                     /locus_tag="ACHDLD_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN3363314.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHDLD_001614"
     CDS             1139..3784
                     /locus_tag="ACHDLD_001614"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN3363315.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHDLD_001615"
     CDS             4094..5407
                     /locus_tag="ACHDLD_001615"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN3363316.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHDLD_001616"
     CDS             5397..6050
                     /locus_tag="ACHDLD_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3363317.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="ACHDLD_001617"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="ACHDLD_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="ACHDLD_001618"
     CDS             6787..7872
                     /locus_tag="ACHDLD_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363318.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="ACHDLD_001619"
     CDS             7869..9761
                     /locus_tag="ACHDLD_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN3363319.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="ACHDLD_001620"
     CDS             9768..10145
                     /locus_tag="ACHDLD_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFN3363320.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="ACHDLD_001621"
     CDS             10299..11081
                     /locus_tag="ACHDLD_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFN3363321.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACHDLD_001622"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACHDLD_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFN3363322.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="ACHDLD_001623"
     CDS             complement(11996..12055)
                     /locus_tag="ACHDLD_001623"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFN3363323.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="ACHDLD_001624"
     CDS             12447..13109
                     /locus_tag="ACHDLD_001624"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3363324.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="ACHDLD_001625"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="ACHDLD_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            13637..13837
                     /locus_tag="ACHDLD_001626"
     CDS             13637..13837
                     /locus_tag="ACHDLD_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3363325.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         4797 a         2128 c         2862 g         4131 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaaga
//
LOCUS       DAZAOG010000067        13810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000067 DAZAOG010000000
VERSION     DAZAOG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.067"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACHDLD_001627"
     CDS             complement(221..1423)
                     /locus_tag="ACHDLD_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363326.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACHDLD_001628"
     CDS             complement(1426..1620)
                     /locus_tag="ACHDLD_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3363327.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACHDLD_001629"
     CDS             complement(1667..2515)
                     /locus_tag="ACHDLD_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN3363328.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACHDLD_001630"
     CDS             complement(2720..3070)
                     /locus_tag="ACHDLD_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363329.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACHDLD_001631"
     CDS             3248..4111
                     /locus_tag="ACHDLD_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3363330.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACHDLD_001632"
     CDS             4735..5727
                     /locus_tag="ACHDLD_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363331.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACHDLD_001633"
     CDS             5743..6627
                     /locus_tag="ACHDLD_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3363332.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACHDLD_001634"
     CDS             6676..8283
                     /locus_tag="ACHDLD_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3363333.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACHDLD_001635"
     CDS             8284..9768
                     /locus_tag="ACHDLD_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN3363334.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACHDLD_001636"
     CDS             9945..10937
                     /locus_tag="ACHDLD_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3363335.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACHDLD_001637"
     CDS             10989..11858
                     /locus_tag="ACHDLD_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3363336.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHDLD_001638"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHDLD_001638"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN3363337.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZAOG010000068        13683 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000068 DAZAOG010000000
VERSION     DAZAOG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.068"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..475
                     /locus_tag="ACHDLD_001639"
     CDS             137..475
                     /locus_tag="ACHDLD_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363338.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ACHDLD_001640"
     CDS             complement(680..1255)
                     /locus_tag="ACHDLD_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFN3363339.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ACHDLD_001641"
     CDS             complement(1987..2568)
                     /locus_tag="ACHDLD_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3363340.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ACHDLD_001642"
     CDS             complement(2751..3377)
                     /locus_tag="ACHDLD_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN3363341.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ACHDLD_001643"
     CDS             complement(3399..4730)
                     /locus_tag="ACHDLD_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN3363342.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ACHDLD_001644"
     CDS             complement(4885..5286)
                     /locus_tag="ACHDLD_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3363343.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ACHDLD_001645"
     CDS             complement(5372..5578)
                     /locus_tag="ACHDLD_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363344.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ACHDLD_001646"
     CDS             complement(5673..7175)
                     /locus_tag="ACHDLD_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN3363345.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ACHDLD_001647"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ACHDLD_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3363346.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ACHDLD_001648"
     CDS             complement(7746..8363)
                     /locus_tag="ACHDLD_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3363347.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ACHDLD_001649"
     CDS             8584..9957
                     /locus_tag="ACHDLD_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3363348.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ACHDLD_001650"
     CDS             10032..11144
                     /locus_tag="ACHDLD_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3363349.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ACHDLD_001651"
     CDS             complement(11216..12853)
                     /locus_tag="ACHDLD_001651"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN3363350.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ACHDLD_001652"
     CDS             complement(13021..13629)
                     /locus_tag="ACHDLD_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3363351.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3687 a         2884 c         2452 g         4660 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atc
//
LOCUS       DAZAOG010000069        13585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000069 DAZAOG010000000
VERSION     DAZAOG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.069"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..254
                     /gene="yaaA"
                     /locus_tag="ACHDLD_001653"
     CDS             12..254
                     /gene="yaaA"
                     /locus_tag="ACHDLD_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN3363352.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            241..1365
                     /gene="recF"
                     /locus_tag="ACHDLD_001654"
     CDS             241..1365
                     /gene="recF"
                     /locus_tag="ACHDLD_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN3363353.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1362..3308
                     /gene="gyrB"
                     /locus_tag="ACHDLD_001655"
     CDS             1362..3308
                     /gene="gyrB"
                     /locus_tag="ACHDLD_001655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN3363354.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3328..5799
                     /gene="gyrA"
                     /locus_tag="ACHDLD_001656"
     CDS             3328..5799
                     /gene="gyrA"
                     /locus_tag="ACHDLD_001656"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN3363355.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6035..6334
                     /gene="rpsF"
                     /locus_tag="ACHDLD_001657"
     CDS             6035..6334
                     /gene="rpsF"
                     /locus_tag="ACHDLD_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN3363356.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6384..6896
                     /gene="ssb"
                     /locus_tag="ACHDLD_001658"
     CDS             6384..6896
                     /gene="ssb"
                     /locus_tag="ACHDLD_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3363357.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6922..7158
                     /gene="rpsR"
                     /locus_tag="ACHDLD_001659"
     CDS             6922..7158
                     /gene="rpsR"
                     /locus_tag="ACHDLD_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN3363358.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7301..9274
                     /locus_tag="ACHDLD_001660"
     CDS             7301..9274
                     /locus_tag="ACHDLD_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN3363359.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9280..9732
                     /gene="rplI"
                     /locus_tag="ACHDLD_001661"
     CDS             9280..9732
                     /gene="rplI"
                     /locus_tag="ACHDLD_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN3363360.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10006..11373
                     /gene="dnaB"
                     /locus_tag="ACHDLD_001662"
     CDS             10006..11373
                     /gene="dnaB"
                     /locus_tag="ACHDLD_001662"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN3363361.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12153..13445
                     /locus_tag="ACHDLD_001663"
     CDS             12153..13445
                     /locus_tag="ACHDLD_001663"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN3363362.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4521 a         2465 c         2992 g         3607 t
ORIGIN      
        1 agggatgggc attgaaacaa caagtcattc taaatacgga attcatgacg ttgggccaaa
       61 tgttgaaaga agtcacagtt atcggtagtg gcggacaagc aaaatggtat ttggcagaaa
      121 acactgtgtt agtcgatggc gaaccagaaa atcgtcgtgg acgcaaactt tatcccggca
      181 tgatggttga agttccagaa gtaggaactt tttttatggt aaaaaaagga aacgacgata
      241 atgaggctga ataagctgta tttaaaaaat tatcgaaatt acgaagagct gaacatcgaa
      301 ttttccaaga acctcgtcat ctttttagga gaaaatgcgc aaggaaaaac gaatcttttg
      361 gaaagtatct acgtattagc tatgacgcga agtcatcgga caagtagtga gcaggaattg
      421 attggctgga ctgacgatca ggcgatgatt caaggagaga tcacaaaagg ttcatccact
      481 cttccattag aaatcttatt atctaaaaaa ggccgaaaaa ccaagatcaa ccatatcgaa
      541 cagaaaaagt taagtactta cgtgggacaa atgaatgtca ttttatttgc accagaagat
      601 ctttctttag taaaaggcag cccgcagacc aggcggaagt ttttagatat ggaattaggc
      661 cagatcaatc caatctatct ttatgatctt gtccaatacc aagcaatttt gaaacaaaga
      721 aaccagtact tgaagcaatt gaatgaaaaa aaacaaactg atttgcttta tttggatatt
      781 ttgtccgaac aactagcagc gtttggcagc aaagtattga aagcaagagc acagttcgtc
      841 aagcgattgg agttttgggc taattctttg catcagcaaa tcacccatca aaaagagcag
      901 ctggaaatcg agtatctcac cgcagtcgat tctttagaaa cacatacgca agagcaaatc
      961 caagaacaat ttctcgcact tttgaaccaa aataaaaaga aagacctttt tcggggaaca
     1021 acgacagttg gtcctcatcg tgacgatctt tcctttttta ttaatcagaa aaatgtgcag
     1081 acttatggat cgcaaggaca gcaaagaaca actgcactaa gtgtcaaatt agcagaaatc
     1141 gatttgatta aagaagagac aggggaatat ccaatccttt tgttagatga tgtgatgagc
     1201 gagctggatg ataatcgtca actccatctg ctagaaacaa tcgaaggaaa agttcaaacg
     1261 ttcttaacaa caacgacact tgaccatgta aaaaataaaa tgactgttga acctgagatt
     1321 ttttatgttc aacaagggaa actaaaagga gaagaaactg aatgacagaa gaaagaagtt
     1381 tagtagaacg cgctaaagag tatgatgcca gtcagattca agtacttgaa ggacttgaag
     1441 ccgttcgtaa acgacctggc atgtatatcg gttcgactag ttctgagggt ctgcatcacc
     1501 ttgtttggga aattgtcgat aattcgatcg atgaagtatt agcaggtttt gcaacgaaaa
     1561 tccatgtgat tattgaaaaa gacaacagta tcaccgttat tgacgacggt cgggggatac
     1621 ctgtagatat ccaggcaaaa actggacgtc ctgcagttga aacggtcttt actgttttac
     1681 atgctggagg aaaattcggc ggtggtgggt acaaagtttc tggtggactt cacggagtag
     1741 gatcttctgt tgttaacgcg ctttctacac agcttgatgt caaagtatac aaagaaggaa
     1801 atgtgtacta tcaagaatat cgtcgtggag ctgtagtgga cgacttaaag attatcgaac
     1861 aaacggatcg ccatggaaca acggttcact ttacgccaga ccctgaaatt tttacagaga
     1921 caactgagtt tgatttttca aaattggcta cccggatccg tgaacttgct ttcttgaacc
     1981 gcgggatgcg tatatcgatt gaagacaaac gagaagaaga accagtcatc aatgaatacc
     2041 attatgacgg cgggattaaa agttatgtcg aatacttgaa tgcaaacaaa accgtgattt
     2101 ttccagaacc tgtatatttg gaaggtgaac agcaagatat cgcagttgaa gtctctatgc
     2161 aatatacaga cggctaccat tccaatatca tgagttttgc caacaatatc catacgtatg
     2221 aaggcggaac acatgaatca ggattcaaga ctgctttaac tcgtgtgata aatgattatg
     2281 cccgaaaaca aaagctgatg aaagaaaatg acgacaactt gaccggtgaa gacgtgcgag
     2341 aaggattgac agctgttatt tctatcaagc atcctgaccc tcaattcgaa ggacaaacaa
     2401 aaacaaaatt gggcaattcc gaagtacgga cagtcactga tcggttattt agcgaacatt
     2461 tcatgaaatt cttgctggaa aacccaagtg tgggccgtca aattgttgaa aaaggattac
     2521 tagcttccag agctcgtcta gcagcgaaac gtgcgcgtga agtaacgaga cgtaaaggtg
     2581 ctttagagat tagtaatctg ccaggaaaat tagcggactg ctctagcaac gatcctgaaa
     2641 aatgcgaatt gttcatcgtc gaaggagatt cggccggcgg atcagctaag caaggtcgta
     2701 atcgagagtt tcaagccatc ttgcctattc gtgggaaaat cttgaatgtt gaaaaagcaa
     2761 gcatggacaa gatcttggca aatgaagaga tccggtcttt atttactgct atgggtacag
     2821 ggtttggaga agattttgat gtatcgaaag ctcgctacca caaattagtt atcatgactg
     2881 atgccgatgt cgacggcgcg catatccgta cattgctgct gactcttttt tatcgttaca
     2941 tgcgtcctat tgttgaagct ggatacgtct atattgctca gccaccttta tacggggtaa
     3001 aacaagggaa gaatattacg tatgtacaac caggaaaaaa tgcagaagaa gagttgaaac
     3061 aagtcgttgc ttcacttcct gcctcaccaa aaccaagcgt tcagcgttat aaaggacttg
     3121 gagaaatgga tgatcatcaa ctatgggaaa caactatgga cccaagcaat cggatgatgg
     3181 cccgcgtcag tgtggacgat gctattgcag cagatcagat atttgaaatg ttgatggggg
     3241 atcgagtaga accccgtcgt gcattcatcg aggaaaatgc acactatgtg aagaacttag
     3301 atatttaatc atgaaggagg aaaaagaatg agtgaagaaa tcagagaaaa catccatgat
     3361 gtcaatctaa ccagcgaaat gaaagactcc ttcatcgatt atgcgatgag tgttattgtc
     3421 gcacgtgcac ttcctgatgt cagagatggt ctgaaacccg ttcatcgccg tattttatac
     3481 ggcatgaacg aattaggtgt aacaccggat aaagcccata aaaaatctgc cagaatcgtc
     3541 ggagatgtca tgggtaaata ccatcctcat ggagactatg cgatctatga gtcaatggta
     3601 cggatggcac agccattcag ctaccgttat atgctagtgg atggtcatgg aaactttggt
     3661 tctgttgacg gtgacggtgc cgccgctatg cgttataccg aagcacggat gagcaaaatc
     3721 gctacagaaa tgctgcgtga tatcaataag aacactgttg atttccaaag caactatgat
     3781 gatacagaaa aagaaccagt tgtactacct gctcgcttcc cgaatctttt agtgaacgga
     3841 acaacgggga tcgccgtagg tatggccacg aatatcccac ctcataactt gtctgaggta
     3901 gtagcggcta tcgatttgct gatggataat cctgatgtga cgaccaatga attgatggaa
     3961 gtattacctg gaccagattt tccaacaggt ggtttggtta tgggaaaatc aggaatccga
     4021 agagcgtatg aaacaggtaa gggttctatt accgtacgtg caaaagttga gattactgag
     4081 atgccaaatg gtaaagaacg gatcatcgtg acagaattgc cttatatggt caataaagca
     4141 aaattgatcg aacggatttc tgaactgcac agagacaagc gtatcgaagg gatcacagat
     4201 ttgcgggacg aatcttcccg tgaaggtatg cggatcgtca ttgacatccg tcgtgacgcc
     4261 agcgcatctg ttatcttgaa taacttatat aaattgacat ccttgcaaaa ttcatttgga
     4321 ttcaatatgt tggcgatcga aaaaggtgtg ccgaaagttt tgagcttgaa acaaattctt
     4381 gaaaactata tcgaacatca gcgcgaagtc atcacaagac ggacagtctt tgaaaaagaa
     4441 aaagcagaag caagggcaca cattttagaa ggtttgcgta ttgctttgga tcatatcgat
     4501 gaaatcattg cgattatccg cgggtctaaa tcagatgacg aagcaaaagc gacgatgatc
     4561 gaacggttcg atttatccga tcgccagtct caagctattt tagatatgcg tttacgccga
     4621 ttgaccggtt tggaacggga aaaaatcgaa aatgaatacc aagaactctt aaaactgatt
     4681 gaagatttaa ctgacattct ggcaagacca gaacgtgtta cagaaatcat caagaatgaa
     4741 ttagcagact tgaatcaacg atttggtgat aaacgacgca cagaattact agtcggtgag
     4801 gtcttaagtc tggaagatga agatttaatc gaagaagaag aaatcgttat tacattgaca
     4861 aacaatggtt acatcaaacg tttggcaaat aatgaattca gagctcaacg aaggggtgga
     4921 cgtggtgtcc aaggaatggg tatccacaat gacgacttcg tcaaaaatct ggtttcttgt
     4981 tctacccatg atacattgtt attctttacg aataacggaa aagtgtatcg ggcaaaagga
     5041 tacgaaattc cagaatacgg gcgcacagcc aaaggaatac caattatcaa tttactaggt
     5101 atcgattcga gtgaaagcat ccaggcaatc attgcggtga cgggagaagc agaagaaggc
     5161 cactatctct tctttacgac gcgtcaaggt acagtaaaac gtacttctgt cactgctttt
     5221 gctaacatcc gaagtaatgg tctgatcgca attggtttga aagaagacga tgagctcgtc
     5281 aatgtgctac tgacagatgg tcaatctaat atcattattg gaacacataa cggctactct
     5341 gtcactttca aagaagaagc agtccgcgat atgggacgta cagcttccgg ggttagagga
     5401 atacgcctgc gtgaggaaga ttatgtcgtt ggggcagcct taatgaagga agatcaagaa
     5461 gtattagtga tcacagaaaa aggttacgga aaacggacaa aagtatcgga atatccagtg
     5521 aaaggccgcg gaggtaaagg gatcaaaacg gcgaacatca ctgaaaaaaa tggtccacta
     5581 gctggattga ccactgtttc tggagatgaa gacatcatgc tgattacaga taaaggtgtc
     5641 attatccgtt tcaacgtatc gactgtttct caaactggac gttcaacatt aggcgtacgt
     5701 ttgatgaaga tggaagctga tacgaaagtt gtcacgatgg cagcagtcga accagaagct
     5761 gcagaacaaa cagttgaaga acaaggatca gaagaatagc catcaagact aattgtgtaa
     5821 gctagcaaga atgaccgatt aaaaaatctg gttattcctg ctggcttttt tgattagttg
     5881 aatttctctt aaaatattcg aaactgttca ttctgtattg cttttgacca aaatagagtg
     5941 tataatcaga gcttgtgagt agtctaatta attagataac tctccttgct cttaggaacc
     6001 taagagccga aagtccacag ggaggtgaat atcaatggaa aacacgaaat atgaaatcat
     6061 gtacattatt cgtccaaaca ttgatgaaga agcaaaaact gctttaatcg aacgtttcga
     6121 tgcaatcttg aaagataacg gagcagaagt tatcgaatca aaagattggg aaaaacgccg
     6181 tttcgcttat gaaatgaacg gttaccgtga aggtatctat cacatcgtta aagtatcttc
     6241 tccatcatca gcaaatgctg tcaatgaatt tgaccgtctt gctaaaatca acgacgatat
     6301 cattcgtcac atgattgtta aagaagaagc ttaattgttg tttcatgtga aacaatagaa
     6361 aacgatgaga ggagatgact attttgatta ataatgttgt attagttgga cgtctgacga
     6421 aagatccaga tttgcgttac acagcaagtg gtacggcagt cgcaacattt actttggctg
     6481 tgaatcgtaa ttttacaaac caaagcggca atcgcgaggc tgattttatc aactgtgtga
     6541 tttggcgcaa atcagcagaa actttagcaa actatgctcg taaaggaaca cttttaggtg
     6601 ttactggacg tatccaaact cgttcttatg ataatcagca aggacaacgt gtatacgtga
     6661 cagaagtagt agctgacaac ttccaattgc tggaaagtcg ttctgcatct gaaaatcgcc
     6721 aacaaagcgg cggattccaa agttctggtc aatcagctgg cggatttggc ggaaacaata
     6781 actctaatca aacatctcaa tcatccaacg gtatgcctga tttcgaccgt gatacttctg
     6841 acccattcgg ctcaagttca acgatcgaca tttcagacga tgatttacca ttctaaacga
     6901 atttgatagg agggaaatag aatggcacaa caaagaagag gcggacgtaa acgtcgtaaa
     6961 gtagactaca ttgctgctaa ccatattgaa tatatcgatt ataaagatat cgaattacta
     7021 aaaagattta tttcagaacg cggtaaaatc ttgccacgtc gtgtaactgg tacaggcgct
     7081 aaaaaccaac gtaaaattac tgttgcaatc aaacgcgctc gtatcatggg cttgctacct
     7141 ttcgttagcg aagactaata aatcctagga attgaatgac tgatcctttt gatcagctat
     7201 tcaattcctt tttttctgta aaaataacct ctattcgttt agtcgctcga atgacgaaag
     7261 ttatggtaaa atcaaattag tcatatatgg gaggaaaatt atggaaaata aaaacaatca
     7321 tttatccccg aaacctctct tccttatcct gtttatatta atcgaagtat tggtagtctt
     7381 aattttacca tttcgttttg ttagcgcttc ggtcattcta ttgatgaatt tatggggtgt
     7441 tcgctattgg ttgaaaatga aacgttggct tattttaaat aaacgtgaaa aaatccagca
     7501 ggcttcgact gtagctgaaa ccaatctagc ttatgtctca gaagtcatgc ctgtaggagt
     7561 gattgcttat cagccatcaa gccaaaaaat cgaatggctc aatccattag cattgaaaat
     7621 caaagatcaa agtggattaa cagaggaaga attgctagat acttttttta ctgcaaaaga
     7681 aaaaaagaaa aaccaagtct atctagcaga taaaacattt cagtttgata tggatacagt
     7741 aaagggtgtt gtttatttta atgatattac ccaaaaccag ttgttaaaac tccatcagac
     7801 gaacagtcag ccggtgattg gtattttatc tattgataac tatgcagatg ccattgataa
     7861 attggatgac aaagagattt cttatttgaa ctctttcgtc actacactag tttctgactg
     7921 gatgaatgaa taccatgtct ttttcaaaag aatcaacgca gaacggtttt tcttcgtagc
     7981 acagatgatc gatctgaaaa agatgatgga taaaaaattt gatttgctgg atcgctttcg
     8041 agcagaagct gaaaaacaga gctcgcctat cacccttagt atggggattg cttatggctc
     8101 aagaaccctc gatgaaatcg gtggagaagc tcagaataat ttagatgttg ctttagtccg
     8161 tggaggcgac caagttgtcg tgaaagaggt aaaagaagga gcaaaacctg tttattatgg
     8221 ggggaaatct gctaagaatg tcaaacgcac gcgtgttcgt tctcgagcga tgagtaccgc
     8281 tttgcgtcgg atttttgatg aaacaggcga tgtgtttatc atggggcaca agtttccgga
     8341 tatggatgct ttaggctctg cttttggtgt cagttgtctg gcacgcttca accagaaaaa
     8401 agcttacatc gttatcaacg agaacgagat cattccagat gtagaacggt gtcttgaaga
     8461 aatccataag catcctgaat tagaaatgca actaatctct ccagaacgtg ccatagagct
     8521 gaaaaaagaa gaggacctcc tcgtgatggt ggattaccat aaaccttctc tatcgatctc
     8581 tcagggccta tatgagcaat ttgataagat tgtgatcatt gatcaccatc gtcgtgggga
     8641 agagtttcca agtaaacctc tgctgactta tatcgaatct tcagcttctt cagcttcaga
     8701 gctagtttct gagctgatcc aataccaaag cagcaaaaca aatcgcttag atcgttttga
     8761 ggccacatta ttgcttgcag ggatggttgt agatacaaat agtttcaatg tccgtacaac
     8821 tgcacgaact tttgacgttg ccagctattt aaggtcttgt ggagcagacg catccttagt
     8881 acaatattta ttgagctcgg atttagcgac atacttggag atcagcaatt tgattgctgg
     8941 aagcgactat gtgacttctg atattgttgt ggcggcaggg acagacgaaa aagagtataa
     9001 tagtgttata gcagcaaaaa cagcagatac attgctttcg atgatcaata tcaatgcagc
     9061 tttcgtcatt acaaagcgga cagacggctt gattgggatc agtgccagaa gtaccggcac
     9121 gatcaacgtc caaatcatca tggaatcctt aggtggcgga ggacacttca caaacgcagc
     9181 tactcaactg gaaaatacga cagttgagga aacaaaacag aagctatttg ctgcaatcaa
     9241 agaaaatatg aatcaaatat atgataagga gtaatccaaa tgaaagtaat ttttttacaa
     9301 gatgtaaaag gaaaaggtaa aaaaggggac atcaaagaag ttccagcagg ttatgcacaa
     9361 aattatctaa tcaaaaataa cttggcaaaa gaagcaacga aaagcagtat cagtcagtta
     9421 gcaggacaaa agaaagcaca agaaaaacac gaagctgaaa tcttggaaga agctaaaaag
     9481 ttaaaagaat tcttggaaaa agaagatact gtagtcgaat tgaaggcgaa agcaggggaa
     9541 gacagccgtt tgtttggttc gatcccttca aaacaaattg ctgaagggtt aaacaaacaa
     9601 tacaaaatca aattagataa acgaaaaatc gaattagaaa atccgatccg ttcactaggc
     9661 tacacaaacg tacctgttaa gcttcatcat gaggtgacgg ctacgatcaa agttcatgtc
     9721 gtacaagaat aacaaatctt gatagcgatg ttcagctggt aagtataacc agaaggaaaa
     9781 aagacaaacc gaaatttatc tgagaaaaca gctgcagcaa tgaaagcaaa agctgcaaat
     9841 cgatttcaga ttgatttcgg tttctttttt gggaacagca aaatagcata aagaaaacga
     9901 gtataataaa agagaactat ttttttatta cactttcggt acgaatgatg acttttctct
     9961 cattttcttg taaaatgaag caattgaaga caaggagcga tacgtatgaa tgaagtgtgg
    10021 caggatcgaa tcccaccgca aaatattgaa gctgaacaag cggttttagg ctctgttttt
    10081 cttgacgcag aaacgatcat tgatgccatg gagtacatag agccaaaaga cttttatcgt
    10141 cgtggccatc aaattatttt tgagacaatg atcgagttaa atgatcgtaa tgaagcgatt
    10201 gacgtggtta ctgtcaaaga ccggctggaa caagccaatt tgttagaaga tgccggtggt
    10261 ttaagttatc tctcagactt ggcattagca gttccgacag cagcaaatat cgtctactat
    10321 gcgaaaatcg ttgaagaaaa atcattgctg cgtacattga tccagacagc aacaggaatc
    10381 gttacgaaag gattcgagca aggagaagaa gttcaaacaa ttttagatga tgccgaacgc
    10441 agtatcttgg aggtttcgga aaaaagaaat cgaagcggct ttttatctat tgctgaagta
    10501 ctgaactcca cgattgaaaa tatcgatcga ctagctcaaa acaatgaaga aatcacaggt
    10561 ctaccaactg gctatcaagg tctggataaa atgacagcag gtttccagcc cgaagaacta
    10621 atcattcttg ctgcacgtcc agcggtaggt aaaacagcgt ttgcattgaa tattgctcaa
    10681 aatgtaggaa caaaaacaga tcgttctgtt gcgatattta gtttggaaat gggggcagaa
    10741 tctcttgtaa atcggatgct ttgtgcagaa ggttcgattg aggccagcca tttacgtaca
    10801 ggtcagcttt cggaagaaga atggtcgaat ctgatcgttg cgatggggag tctttctcga
    10861 gcaaatattt ttatcgatga tacaccaggg atcaaaatct ctgaaatccg tgcgaaatgt
    10921 cggaaactag cgcaagaaaa aggcaatcta ggcttgatat tgatcgatta tttgcagttg
    10981 attgaaggta acggtaaaga gaaccgtcaa caagaagtat cagatatctc tcgccaactg
    11041 aaaaaactgg cgaaagagct gaaagtacct gtcattgctc tttcacagtt atctcgtgga
    11101 gtagaacagc gacaagacaa acgaccagtg ctaagtgata ttcgtgaatc cggatcaatt
    11161 gaacaagatg cggacatcgt agccttcttg tatcgagatg attattatga aagagaaggc
    11221 ggagatgacg atgatggaaa cgagccaaaa aatgacaacg tcatcgaagt catcatcgag
    11281 aagaaccgaa gcggtgcacg aggaacagtt gaactactgt ttatcaaaga atacaataag
    11341 ttttcgtcgc tttcgccaag agaagaattt taaagaagag gttgtgagcc ttgagcaatc
    11401 ccgagagctc tcgtaagaag atgcaaggag taaccgcagg aacgactaga gtacttaatt
    11461 taaacagatc tccaaaggga ttgtgacaaa agtcttgtca cagtcccttt ttccgaataa
    11521 acggtgttca atcagctgac ccttggtatt agctcagcaa acgtcaaaat atgaggaact
    11581 attttgacgt ttgtttctct aatactcagg tcaaaacgct gatcttacga cctcttttcc
    11641 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatcatc aaacatgaga
    11701 agcagttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctt
    11761 tcttaaacgg tgttcaaaag cttgcatctg cggtaatacg cccaatcaaa cgaaaaatat
    11821 gagaagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacaacc
    11881 tctgattcac ggtgttccaa aagtagcttc ttcgaaaaat aagtcgaaat ttcacaaaaa
    11941 tttgagaagc aattttcgta cattccttct tatttcttga agctgaccac ttctgttaca
    12001 acctcttttt taataaccat tctaaaaaga taaagttcgt tttttaagcg tttttataaa
    12061 ataaaaataa aaatcattcg attttatttg aaatatgtat ctattattga tacaatggtt
    12121 caggattaaa taatatagaa cgaggtgttc acatgtcatc tgtagtagta gtaggtacgc
    12181 aatggggcga tgaagggaaa ggaaaaatca cagactttct cagtgaaaat gctgaagtga
    12241 ttgcacggta ccaaggtggc gacaatgcag ggcacacaat taagtttaac ggtgtgactt
    12301 ataaactgca tttgattcct tctgggattt tttataaaga caagatcagt gtgatcggaa
    12361 acggcgttgt agtcaatcct aaatcattgg ttaaagaatt agcttattta catgaaaaag
    12421 gtgtagaaac aagtaatttg cgtatttctg accgcgcgca cgtaatcttg ccttatcata
    12481 tcaaattgga ccaattacaa gaagatgcta agggcgaaaa taagattggt acaacgatta
    12541 aaggaatcgg acctgcctat atggataaag cagctcgtgt aggtatccgt attgcagact
    12601 tattagataa agaaattttt gaagaacgtc ttcgtttgaa tctagatgac aaaaaccgtc
    12661 aatttgtaaa aatgtttgat tcagaaccac ttgattttga cgatattttt gaagaatatt
    12721 atgaatacgg acaacaaatc aaacaatacg tcacagatac ttccgttatc ttgaatgatg
    12781 ctttggatgc aggcaaacgc gtgttgtttg aaggcgcaca aggtgtaatg ctggatatcg
    12841 accagggaac ttatcctttt gttacttctt ctaacccagt agccggtggg gtaacaatcg
    12901 gaagcggtgt agggccttca aaaatcaata aagttgtcgg cgtctgcaaa gcctatacgt
    12961 cacgtgtcgg tgatggacca tttccaactg aattatttga tgaaacagga gaacaaatcc
    13021 gcgaagtagg acgagaatac ggtacgacaa ctggtcgtcc gcgtcgtgta ggctggtttg
    13081 attctgtagt tatgcgccat tctaaacgag tttcaggtat taccaaccta tctttaaact
    13141 cgatcgatgt attaagtgga ttggaaacag taaaaatttg tacggcttat gaattagatg
    13201 gcgaactaat ctaccactat ccagcaagct tgaaagagtt gaaccgctgc aaaccaatct
    13261 atgaagaatt gccaggctgg tcagaagaca ttacaggatg caaaacactt gcagaactgc
    13321 cagaaaacgc acgtaactat gtacgtcgta tctcagagct tgttggtgta cgtatctcaa
    13381 cattttctgt cggacctgat agaacacaaa caaacatctt agaaagcgtc tgggcgcaaa
    13441 tctaaggata aaaacgacga ctgaatcagg acgttcttac cagagcgagt cgaagtgttt
    13501 atctatacga taataataac ttgaacactg tttattcaga ataagggact gtgacaaaaa
    13561 ccgtgtcaca gtcccttttt ccgaa
//
LOCUS       DAZAOG010000070        13520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000070 DAZAOG010000000
VERSION     DAZAOG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.070"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..639)
                     /locus_tag="ACHDLD_001664"
     CDS             complement(277..639)
                     /locus_tag="ACHDLD_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363363.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="ACHDLD_001665"
     CDS             complement(713..1093)
                     /locus_tag="ACHDLD_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN3363364.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="ACHDLD_001666"
     CDS             1175..2350
                     /locus_tag="ACHDLD_001666"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN3363365.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="ACHDLD_001667"
     CDS             complement(2427..3011)
                     /locus_tag="ACHDLD_001667"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3363366.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="ACHDLD_001668"
     CDS             3212..4210
                     /locus_tag="ACHDLD_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3363367.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="ACHDLD_001669"
     CDS             4322..4831
                     /locus_tag="ACHDLD_001669"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN3363368.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="ACHDLD_001670"
     CDS             complement(4861..5520)
                     /locus_tag="ACHDLD_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN3363369.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="ACHDLD_001671"
     CDS             complement(5522..6286)
                     /locus_tag="ACHDLD_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN3363370.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="ACHDLD_001672"
     CDS             complement(6294..6971)
                     /locus_tag="ACHDLD_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN3363371.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="ACHDLD_001673"
     CDS             complement(7008..8393)
                     /locus_tag="ACHDLD_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN3363372.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="ACHDLD_001674"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHDLD_001674"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHDLD_001675"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHDLD_001675"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN3363373.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..10189
                     /locus_tag="ACHDLD_001676"
     CDS             9332..10189
                     /locus_tag="ACHDLD_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363374.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10270..10605)
                     /locus_tag="ACHDLD_001677"
     CDS             complement(10270..10605)
                     /locus_tag="ACHDLD_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363375.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10672..12042)
                     /locus_tag="ACHDLD_001678"
     CDS             complement(10672..12042)
                     /locus_tag="ACHDLD_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN3363376.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12120..12656)
                     /locus_tag="ACHDLD_001679"
     CDS             complement(12120..12656)
                     /locus_tag="ACHDLD_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3363377.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12965..>13520
                     /locus_tag="ACHDLD_001680"
     CDS             12965..>13520
                     /locus_tag="ACHDLD_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363378.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNA"
BASE COUNT         4112 a         2717 c         2212 g         4479 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc gctatctttt
     9541 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9601 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9661 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9721 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9781 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9841 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9901 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9961 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
    10021 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
    10081 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
    10141 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10201 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10261 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10321 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10381 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10441 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10501 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10561 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10621 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10681 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10741 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10801 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10861 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10921 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10981 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    11041 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    11101 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    11161 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11221 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11281 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11341 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11401 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11461 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11521 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11581 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11641 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11701 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11761 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11821 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11881 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11941 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    12001 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    12061 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    12121 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12181 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12241 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12301 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12361 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12421 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12481 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12541 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12601 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt
    12661 acctccgtct cttttcatct acccataaat aaataacata aataattcta aaaatattat
    12721 tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc cgccttaaaa
    12781 acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca aggtaacaaa
    12841 aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa cacagactga
    12901 aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata aaaaggaggt
    12961 atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg tcattcaaaa
    13021 aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac ggacaatcaa
    13081 agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg atgaatttat
    13141 ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta aaaagttagc
    13201 tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta actatttgat
    13261 gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg aacaacttta
    13321 tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct taaaaaaatt
    13381 ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg agttatctat
    13441 ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt ggccattttc
    13501 ctcacttgaa ttaaatgcac
//
LOCUS       DAZAOG010000071        13399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000071 DAZAOG010000000
VERSION     DAZAOG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.071"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..713)
                     /locus_tag="ACHDLD_001681"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACHDLD_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            792..968
                     /locus_tag="ACHDLD_001682"
                     /pseudo
     CDS             792..968
                     /locus_tag="ACHDLD_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="ACHDLD_001683"
     CDS             complement(1144..1713)
                     /locus_tag="ACHDLD_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3363379.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACHDLD_001684"
     CDS             complement(1787..2110)
                     /locus_tag="ACHDLD_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363380.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACHDLD_001685"
     CDS             complement(2264..3076)
                     /locus_tag="ACHDLD_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363381.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACHDLD_001686"
     CDS             3403..4278
                     /locus_tag="ACHDLD_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFN3363382.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACHDLD_001687"
     CDS             4292..4615
                     /locus_tag="ACHDLD_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363383.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACHDLD_001688"
     CDS             4612..5364
                     /locus_tag="ACHDLD_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN3363384.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACHDLD_001689"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACHDLD_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHDLD_001690"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHDLD_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN3363385.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="ACHDLD_001691"
     CDS             complement(9828..12014)
                     /locus_tag="ACHDLD_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3363386.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHDLD_001692"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHDLD_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN3363387.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="ACHDLD_001693"
     CDS             complement(12236..12676)
                     /locus_tag="ACHDLD_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN3363388.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="ACHDLD_001694"
     CDS             complement(12750..13289)
                     /locus_tag="ACHDLD_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN3363389.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DAZAOG010000072        13212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000072 DAZAOG010000000
VERSION     DAZAOG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.072"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHDLD_001695"
     CDS             147..1016
                     /locus_tag="ACHDLD_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3363390.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHDLD_001696"
     CDS             1225..2250
                     /locus_tag="ACHDLD_001696"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3363391.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHDLD_001697"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHDLD_001697"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN3363392.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACHDLD_001698"
     CDS             3333..5036
                     /locus_tag="ACHDLD_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3363393.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHDLD_001699"
     CDS             5038..7071
                     /locus_tag="ACHDLD_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN3363394.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHDLD_001700"
     CDS             7082..7522
                     /locus_tag="ACHDLD_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3363395.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHDLD_001701"
     CDS             complement(7722..8849)
                     /locus_tag="ACHDLD_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363396.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHDLD_001702"
     CDS             complement(8852..9529)
                     /locus_tag="ACHDLD_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3363397.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHDLD_001703"
     CDS             9793..11559
                     /locus_tag="ACHDLD_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN3363398.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHDLD_001704"
     CDS             11574..12011
                     /locus_tag="ACHDLD_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3363399.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHDLD_001705"
     CDS             12024..13175
                     /locus_tag="ACHDLD_001705"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN3363400.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZAOG010000073        12906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000073 DAZAOG010000000
VERSION     DAZAOG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.073"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1134)
                     /gene="rpoD"
                     /locus_tag="ACHDLD_001706"
     CDS             complement(25..1134)
                     /gene="rpoD"
                     /locus_tag="ACHDLD_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN3363401.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1158..2999)
                     /gene="dnaG"
                     /locus_tag="ACHDLD_001707"
     CDS             complement(1158..2999)
                     /gene="dnaG"
                     /locus_tag="ACHDLD_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN3363402.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3181..5496)
                     /locus_tag="ACHDLD_001708"
     CDS             complement(3181..5496)
                     /locus_tag="ACHDLD_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3363403.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5681..6541)
                     /locus_tag="ACHDLD_001709"
     CDS             complement(5681..6541)
                     /locus_tag="ACHDLD_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN3363404.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6600..7232)
                     /locus_tag="ACHDLD_001710"
                     /pseudo
     CDS             complement(6600..7232)
                     /locus_tag="ACHDLD_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7399..8160
                     /locus_tag="ACHDLD_001711"
     CDS             7399..8160
                     /locus_tag="ACHDLD_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3363405.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8186..10777
                     /gene="mprF"
                     /locus_tag="ACHDLD_001712"
     CDS             8186..10777
                     /gene="mprF"
                     /locus_tag="ACHDLD_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN3363406.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10965..12152)
                     /gene="tuf"
                     /locus_tag="ACHDLD_001713"
     CDS             complement(10965..12152)
                     /gene="tuf"
                     /locus_tag="ACHDLD_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN3363407.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12520..12876
                     /locus_tag="ACHDLD_001714"
     CDS             12520..12876
                     /locus_tag="ACHDLD_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3363408.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3700 a         2703 c         2298 g         4205 t
ORIGIN      
        1 tctcttctgg tcaatagtcc acgcttattc taagaaatct tttaattgtt ttgaacgaga
       61 aggatggcgc aattttctta atgctttagc ctcgatctga cgaatacgtt cccgagtaac
      121 accaaatact ttccctactt cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg
      181 taagcgaagg acattctctt cccgatcagt caatgtatct agtacatctt ccaattgttc
      241 ttttagtaac tcataagctg cgtgttctgc tggacttgtt gcttcctggt cttctatgaa
      301 atcacctaag tgagagtcat cttcttcacc gattggcgtt tcaagggaaa caggttcttg
      361 cgcgattttc aagatttctc ggactttttc tgttggaaga tccatttccg caccgatttc
      421 ttctggcgta ggttctcttc caaggtcttg cagcaattga cgttggatac gtatcaattt
      481 attaattgtt tcaaccatat gaactggaat acggatcgta cgtgcttggt cagcgattgc
      541 tcgagtgatg gcttgacgaa tccaccatgt agcataggtt gaaaacttaa atcctttgcg
      601 gtagtcaaat ttttcaactg ccttcatcag tcccatgttt ccttcttgga tcaagtcaag
      661 gaactgcatg cctcgtccta cataacgttt agcgatactt actactaaac gaaggtttgc
      721 ttctgccaga cgctgtttcg cttcttggtc gccttcttcg attttcaatg ctaacgctac
      781 ttcttcttcc gcagttaaaa gggagacacg gccaatttct ttcagataca tacgaaccgg
      841 gtcattgatt tttacaccgg ttggcgcaga aagatcttct gtttttgctt gttcagcttt
      901 tttctcagca ctcttcaagc tgtgaattga tggatcacca ttttcatcca ccacactgat
      961 acctgcgtct tcaatctttt ggatcaattt gtccatttca tctgcattca aagtgaatgg
     1021 tgtagcaagc tgattggata aatcatcata tacaacttgt cgtttgggtt tgttttcttt
     1081 gatgaaagct gctactgctg cttcatacgt ttttttatct gttccatttg ccataaaaga
     1141 aggcccccct tgagtagcta tttggcttgt tttaactgtt tcgttaaatt gatgatttca
     1201 atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt
     1261 ttttttaatg ttatttcatc agctaagcca gatttgctga tcacatctag caagtcttgg
     1321 atttctcgtg gcgaactttc ttctgccacc ggtaaatatt cgatttccac caccaaccgc
     1381 cttaaatttt catcttgcaa aaagtctaaa aactctgcaa ggttgaattc ataatgttgt
     1441 gagacatatg catcgaataa aaaataaact tctgcatatg catcatgaat aaactgaaat
     1501 tctcgttctt ttaaaatatt acggacagaa acttctttaa atgcacgata taatagaatc
     1561 tgctctgcct tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta
     1621 acctgagtgt gttgactttc ctgaggttct tgacgatttt gctgatttgt ccttcgaaga
     1681 tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa actctgttga cagttgagat
     1741 agatacatat cttgttcgat tggtgaaaca actcggctaa gttcaactag taattcttcg
     1801 aggtattcga tcttgtcttt ttcattatct aaatttttcc cttgttttaa ataacgtgat
     1861 ttaaaagtaa agactgtttc gcgcccatga gccactaatt cttggaaaga tgcttctcca
     1921 tacttacgga gatattcatc aggatcaagc ttttctggaa cgactacgac agaaagtgct
     1981 aatcgactgt tttgccgaag caattcaatc cctcgattgg ttgcttcaaa acctgcgttg
     2041 tctccgtcat agcaaaagac caattctttc gtcagctttt ccatttgttg gatctgttga
     2101 gctgttagac ttgtccccat agaagctaat ccagttttga taccagcctg ccatgcagca
     2161 atcacatcca taaacccttc gaacaacaaa atttctccgt ttttccggat atcgcttctt
     2221 gctttatcaa gattaaataa aacttgtctt ttattgaaca gttctgtttc tggactattc
     2281 aagtatttgg gctgatcttt ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa
     2341 ccgatcgtct ggccttgaga attacgaagt ggaaacatca ctctttgata aaaacgatca
     2401 gacatacggc catcatcatg cgtgacaaac aatccagagc gttctaacaa ttcatttgaa
     2461 attgattcat tcttgaatac ttgctgaaga aaagaacgct cattaggtgc aaaacctatg
     2521 ttgaattctt caattaattc taaagacaac ccacgacttt gcaaatattc taaagctggc
     2581 tgtccagctt ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt ttcatgagca
     2641 gcaatcagtt tccccgtagc agattcagga ttttggacag ctatagtacg ctgacgccat
     2701 tgatcttcta acggtatctg ttcgaagtct gccactttta ccactgcttc tggaaaactt
     2761 agaccttcta gttcttgaag aaaagtaaac acgtttccgc ctttaccaca accaaagcaa
     2821 tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc atgaaaagga
     2881 cacagcccca agtaattttt accagacttt ttcaactgga catattgtcc aatcacctca
     2941 acgatattcg tttgactgcg tactgtttcg ataacctctt gaggaatccg ttgagccatt
     3001 ttttcacccc ctgctctatt gatatctttt gggtttaaca gaagatattt caggagaata
     3061 accggtaaaa aatcgcacta actgaaatca gtgcgatttt aaaggacaca attatacatg
     3121 tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc tcattaagca
     3181 tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt acaatactcg
     3241 aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc ccaaccctaa
     3301 gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta cgatcgtcgt
     3361 tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa ctgccatgca
     3421 actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc cccatgcgaa
     3481 tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg catttggtaa
     3541 agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat cttgttcaaa
     3601 agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga tttggatagg
     3661 gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa atgaataaat
     3721 cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc ggcgtccttc
     3781 aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat tcgcaatctg
     3841 acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa
     3901 aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt tcaattcatt
     3961 taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc gatgagcagc
     4021 ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat cataattcgg
     4081 taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag aaatcacttt
     4141 cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt ttcggattgg
     4201 atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt cgttcaattg
     4261 ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg ctgtttttag
     4321 actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt acacgactcc
     4381 cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt
     4441 cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag tgatgttgtt
     4501 atcactgctt tctgccagat acgtccccat aatttctggg atggctttct cgtacagcgg
     4561 atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc ctgttttatc
     4621 caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt gaacaaggat
     4681 tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta gcaataccaa
     4741 accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa ttttgataga
     4801 agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa
     4861 actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa tagctttgtg
     4921 agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc ggataacttc
     4981 agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc cagtttcttt
     5041 gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc gtccagatat
     5101 aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat ctcctaatac
     5161 cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta cttctgattg
     5221 ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat aaattccaat
     5281 aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac ctacaaataa
     5341 cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat tatcgctgaa
     5401 tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc cagaagccat
     5461 tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca ttttccgcca
     5521 actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa acaacttgtt
     5581 tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc aacctttttg
     5641 tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat tgttcctcca
     5701 aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga caagctagta
     5761 ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa
     5821 ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg taatccatga
     5881 tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca atcacttcaa
     5941 gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac accaaaaaat
     6001 tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg gcaatcagcc
     6061 atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat aataaaaaca
     6121 aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct cttccttttt
     6181 tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca aaaaaccata
     6241 atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt agaaagatgc
     6301 ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg aaattttttg
     6361 taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct gctaatgcac
     6421 caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc acaaaccgta
     6481 acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc cttttttcca
     6541 ctatttgcgc actccttctt tcatttaatt ttattcatga ttgaacattt cctgctattt
     6601 tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac aatatcagga
     6661 taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa ggactggatc
     6721 cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag cacatcttta
     6781 gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac ataggcaata
     6841 taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc ttgtttcttt
     6901 gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc agctggatta
     6961 tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc tgtccctttt
     7021 ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc catatgataa
     7081 ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac cgaacgataa
     7141 gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat gtaaaaaaga
     7201 atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt tgacatcttt
     7261 ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct gtgtacgcct
     7321 taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc gttgtttttc
     7381 agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt aggtgattat
     7441 gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag
     7501 agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg ccgctttttt
     7561 tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga tagccgagga
     7621 catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc cgaggatctg
     7681 aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt tagtgatgga
     7741 gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg attgatttta
     7801 aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat cagtaatatt
     7861 cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa aaatctttta
     7921 gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa aaaaatcaac
     7981 tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat
     8041 atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg acatgaatta
     8101 gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc tgaaacataa
     8161 ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc tgaaaaacca
     8221 cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg tcggtgaatt
     8281 gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg ccactatacc
     8341 tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac ctatggttgg
     8401 ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt acctgtttga
     8461 gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt
     8521 ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga tccaagcatt
     8581 atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc tttctttttt
     8641 acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat ggttgatcgg
     8701 cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga aaggattcct
     8761 tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt tggaatggac
     8821 cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga ttgatctgtg
     8881 gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta tgattccagg
     8941 agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg gtgtgccaag
     9001 agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca ttccgttttt
     9061 gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc gttattctgg
     9121 tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct tattatactt
     9181 ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag agtttcgctg
     9241 gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta gtatactatt
     9301 gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac gagcatattt
     9361 gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg attttagttt
     9421 cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta aaaacgaatt
     9481 atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa tcattattgg
     9541 tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt tccctcatcg
     9601 gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt tttcgggatt
     9661 gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc ttcaaggaga
     9721 aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc ttacaactta
     9781 tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt ttgcctatga
     9841 aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata aatgtattgt
     9901 aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag cttttatcga
     9961 agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag agattgttat
    10021 gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt atgtagacct
    10081 gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat
    10141 cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg agcaaatgag
    10201 tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag ggttttcttt
    10261 aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca aaaataccca
    10321 agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata aagtaggaac
    10381 gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg atttcttgtt
    10441 cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt tagggatggc
    10501 ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga ttgcatactt
    10561 ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat ataaaagcaa
    10621 gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt ggattgcata
    10681 tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac aaggtagaat
    10741 aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg atagatgaac
    10801 actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc tacaatctct
    10861 tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg accgctcttt
    10921 tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc ttcgatctct
    10981 gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt
    11041 tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc
    11101 atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac
    11161 tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt tgtcaacacg
    11221 tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc aagtacttgt
    11281 ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt atccccagct
    11341 tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc tttttgtgtt
    11401 tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc acgatcaata
    11461 cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac tggcaatagt
    11521 aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt atccatcaat
    11581 tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc ggaacctttg
    11641 atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg aacttccatt
    11701 tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc
    11761 aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga
    11821 ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc tgtaatcatg
    11881 tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt
    11941 tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc tggagctgca
    12001 tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac tgtagtgatc
    12061 gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt
    12121 ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa ataatataga
    12181 tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact aaatcaaaaa
    12241 gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg tcaatattgg
    12301 tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct tttataaata
    12361 aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt tgaagcaata
    12421 agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca atagacgaaa
    12481 tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta
    12541 cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta tttttacggc
    12601 ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac ccaagcagat
    12661 aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa agatggtgaa
    12721 aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa tgcgtatctt
    12781 aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa ggcttctgaa
    12841 gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa attttattag
    12901 ataaaa
//
LOCUS       DAZAOG010000074        11800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000074 DAZAOG010000000
VERSION     DAZAOG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.074"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..481
                     /locus_tag="ACHDLD_001715"
     CDS             47..481
                     /locus_tag="ACHDLD_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN3363409.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            647..2692
                     /gene="parE"
                     /locus_tag="ACHDLD_001716"
     CDS             647..2692
                     /gene="parE"
                     /locus_tag="ACHDLD_001716"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN3363410.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            2704..5154
                     /gene="parC"
                     /locus_tag="ACHDLD_001717"
     CDS             2704..5154
                     /gene="parC"
                     /locus_tag="ACHDLD_001717"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN3363411.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            5431..7662
                     /gene="pflB"
                     /locus_tag="ACHDLD_001718"
     CDS             5431..7662
                     /gene="pflB"
                     /locus_tag="ACHDLD_001718"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN3363412.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            7906..8667
                     /gene="pflA"
                     /locus_tag="ACHDLD_001719"
     CDS             7906..8667
                     /gene="pflA"
                     /locus_tag="ACHDLD_001719"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN3363413.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            8752..9678
                     /locus_tag="ACHDLD_001720"
     CDS             8752..9678
                     /locus_tag="ACHDLD_001720"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN3363414.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            9860..10753
                     /locus_tag="ACHDLD_001721"
     CDS             9860..10753
                     /locus_tag="ACHDLD_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN3363415.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            10887..11012
                     /locus_tag="ACHDLD_001722"
     CDS             10887..11012
                     /locus_tag="ACHDLD_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN3363416.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11146..11778)
                     /locus_tag="ACHDLD_001723"
     CDS             complement(11146..11778)
                     /locus_tag="ACHDLD_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363417.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         4233 a         1980 c         2502 g         3085 t
ORIGIN      
        1 acaagtataa tagatggaat aaaaagaaaa gcaaggggtg gcagaaatgt ctgtaaaaaa
       61 tccaacacaa gaaacgattt tcaactattt ggaagaagca aaaaatattg ccgtcgtagg
      121 cttgagcaat aaaccagaac gaacaagtta catggttgca caggtattgc aagacaatgg
      181 atataaaatc ataccagtaa atccattatt ggctggtgaa gaaattctag gcgagaaagt
      241 ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc gtagatgtat ttagacggag
      301 tgaattttta cctgaaatag caggtgattt tttgaaaact gatgctaaag tgttctgggc
      361 acaattaggg attgaaaaca aagaagcagc tgagatgcta agaaaagcag gacgtacaga
      421 tatcgtaatg gatcgttgta ttaagattga acttggtaag ttgaaagaaa accaaaaata
      481 aaaatgacca caaagaagag atgatcgata gaggggaatg tcgaaaaagc ggttaggaga
      541 taattaaaat tgatttctcg tctaccctct tttttttcac gaccttttct agtatgataa
      601 tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg tttgccttgg caaaaaaagt
      661 aaacaacgaa tacaatgact cgtccatcca agtattggaa ggattagagg cagttagaaa
      721 aagaccaggg atgtacattg gatccactga cagccgaggc ttgcatcatt tagtctatga
      781 aatcgtcgat aatgcagttg acgaagcatt atcaggttat ggcagtgaga ttgatgtgac
      841 catccatgaa gacaatagta taactgttgc agatagcgga cgtgggatgc ctgtaggaat
      901 gcatgcttcc ggaataccaa cagttgaagt gatttttact gtgctgcatg ctggcggaaa
      961 gtttggtcaa ggagggtaca aaacttctgg tggactgcac ggtgtaggag ccagtgtggt
     1021 aaatgcctta tcgaaatggc taattgtaac gatcgttaga gatggcgtag agtatcagca
     1081 gaaatttaaa aacggcggta aacctgacgg tactttgaaa aagataggaa aaacaaaaaa
     1141 agcaaacggg acgactgtcc attttttacc agatgataca atattcagta caacgaaatt
     1201 ttcatatgaa attttagccg aacggttaag agaatctgcc tttcttctaa aaggcgttaa
     1261 aatctctctt tctgatcttc gtggagaaga accagtcaag gaaatttttc attacgaaga
     1321 aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa gatacattga caccagttgt
     1381 ttatttttca ggagaaaaag aaggtatcga agtagaggtc gcttatcaat acaatgacgg
     1441 ttattcagaa aatgttttat cttttgtcaa taacgttcgg acaaaagatg ggggaaccca
     1501 tgaagctggt atgaaagcgg cgatgacgaa atcttataat gaatatgcga gaaaagttgg
     1561 tttgctgaaa gaacgggaca agaatctgga aggtagtgat ttcagagaag ggttagctgc
     1621 tgtactttct atccgtgtac ctgaaaatct tctgcaattt gaagggcaaa caaaagaaaa
     1681 acttggtacg ccagttgctc gtaccgtagt agataacgtc atcagtgaac aaatgggctt
     1741 ttatttgcag gaaaatagcg aaatgagcca gatgttggtc cgtaaagcaa tcaaagccag
     1801 agaagcgaga gaagcagcac gtaaagccag agaagaaagc cgaaatggga agaagcgtaa
     1861 aaaaggcgaa tctcttcttt caggaaaatt gacacccgca caatcccgaa accctaagaa
     1921 aaatgaattg tatttagtcg aaggggattc tgctggcgga tctgctaaac aaggaagaga
     1981 cagaaagttc caagcaattc tacctttacg aggaaaagtc atcaataccg aaaaagctaa
     2041 aatgcaagat atcttgaaaa atgaagaaat caatacgatg atctatacga ttggtgcagg
     2101 agtcggtcct gagttttcta tcgaagactg taattacgac aaagtaatca tcatgaccga
     2161 tgcggataca gacggcgccc atattcaagt attactgctt actttctttt atcgttatat
     2221 gaaaccgctg attgaagctg ggaaagtata tatcgcgtta ccgcctttat ataaagtatc
     2281 caaaggacaa ggaaagaagc aagtcattga atatgcgtgg acagatgacg aattggcagc
     2341 aatgattaaa aaagtcggca aaggatatat gctgcagcga tataaaggac ttggagagat
     2401 gaatgcggaa caattgtggg aaacaacgat ggatccgact tcccgtacgt tgatccgcgt
     2461 ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc acactgatgg gtgacaaagt
     2521 agaacctcga agaaaatgga tcgaaaatca cgtgcaattt acgctcgaag aagatggcag
     2581 tatcttagat aaaaaagagg atactgaaat ctctccttca gtttctaatg atttattaga
     2641 tgaagaacga gcagataaaa atgaaaacaa tcagttattt gaagtagaat aggaggtcac
     2701 ctgatggaaa atcgacaaga agtacaagaa ttaactttag aagaagtgat gggtgaccgc
     2761 ttcggacgtt attccaaata tattatccaa gaacgtgcac tgcctgatat ccgtgatgga
     2821 ttgaagccag tgcaacgccg gatattattt gccatgaaca aagatggcaa tacgtatgac
     2881 aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca tggggaatta tcatcctcat
     2941 ggtgacatca gtatttatga agcgatggtc cgcatgagcc aagactggaa gttgcgggaa
     3001 gtactgatcg aaatgcacgg gaataacggg agtatggacg gtgatccacc tgctgcgatg
     3061 cgttatactg aagccagatt atccaagtta agtggagaga tgctagcaga tatcgagaaa
     3121 gaaacagtcg atctcgtgtg gaattttgat gatacagaaa aagaaccaac agtccttcca
     3181 gctcgatacc caaaccttct cgtcaatgga tcgacaggta tatctgctgg atatgcaaca
     3241 gaaatcccga ctcataactt agctgaagtg atcgatggca caatctatat gatcgatcat
     3301 ccacaagcga gccttgaaaa attgatggaa tatattcctg gacctgactt tccaacagga
     3361 gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg aaacaggacg aggaaaagtt
     3421 attcttcgtt cgaaaacaaa gatagaggca atgaagggta ataaacagca aatcgtgatc
     3481 acagaaatcc catatgaagt aaataaagcg accttggtga aaaaaatgga tgagatccgt
     3541 ttgaataaga aaatcgatgg aatagctgaa gtacgagatg aaagtgatag aacaggtctg
     3601 caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag gaattttgaa ttatttgttc
     3661 aaaaacactg agctacagat caattacaat ttcaatatgg tcgctatcga ccatatgact
     3721 ccgcatcaag ttggactgaa agacatttta agaagttata tcgagcatcg aaagcaagtc
     3781 ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa aaagacagca tatcgtagaa
     3841 ggattgatga aagctttatc gattctggat gaagtaatcg caacgattcg tgaaagtaaa
     3901 gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc aatttacaga agaacaagca
     3961 gaagcaattg tcacattgca attataccgt ttgacgaaca cggatatcac agagcttcaa
     4021 aaggaatccg aaagcttgat tgcgcaaatc acagagttga ataaaatcct ttcaaatgac
     4081 aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag tgaaaaaaaa ttattcttcc
     4141 gctcgtctaa cgacaatcga agacgaaatc gaagaaatca aaattgatac gcaagtctta
     4201 gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag gctatgtgaa gcgaacaagc
     4261 cttcgttctt atagtgcttc caaaccagaa gaaatcggga tgcgagaagg cgactacttg
     4321 ctatactcag gtgaattgag tacattggac catgttcttc tgatcacaaa taaagcaaat
     4381 gtgatctacc gccctgtcca tgaactaccc gatttgaaat ggaaagatgc tggtgagcac
     4441 atttctcaaa caatctccaa tctgtctgta gacgaatcga ttttagctgt tttcccatat
     4501 caaaaaattc aagcagagaa aacatttgta ttcatcagca agaatggatt gatcaagcaa
     4561 acacgaatga cagagttcga accatggcgc acctataaga gccgtccgct cagctgtatg
     4621 aagctaaagg cggaagaaga tgaactgatt gctgtgtatc tgaaagaaga tcagccaggc
     4681 ttagatgttt tcttagtaac gcatcaagga atgggtttgc gctatccttt aacagatgtt
     4741 cccgttgttg gagcaaaggc agctggagtg aagtctatga atttgaaaga tggagatagg
     4801 attatcaacg ggctgcttgt gttagcagaa ggtgatacgc cgatcgtaat cgttactcag
     4861 cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc aaacttcacg tgcgaagcgg
     4921 ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc gtattatcta tatgtcggaa
     4981 ggaagatcaa aagaaataac cttgatgaat caaaaagggc agcagttaac gattgaccca
     5041 actgatttcc ctataggtga tcgaacatcg aatggatcat ttgcgatgga tgagaaaaaa
     5101 ggtggagaag tcatcgaagt tatcgatgct cctgagattt tcatcgagtc gtaactgttg
     5161 tagtatttta atgaagagac gccatctgta ttaatacagg tggcgtctct ttttcattca
     5221 tttttaataa atccttatac gtttgcaaaa aaagatgaaa gtgttcacaa tgcttaaatg
     5281 ttggtatgtc aacgttgatt taaatatatg taaataaaac ataaaaattg ttaaataaaa
     5341 aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac taatcacgta aaaactgtta
     5401 cataaaacag aaatttagga ggtccataca atggagcaat ggaatggatt taaaggtacc
     5461 aaatggaaag aaggcgtaga cactagagac tttattcaaa ataactacac tgaatatcgc
     5521 ggtgatgaca gctttttgga aggcccaaca gaagcaacag aaaaattatg ggatgaattg
     5581 caaaagttaa atgatatcca attcgagcgt aacggtgtct atgatatgga cacagaaatc
     5641 gtatcaacta ttacttcaca cgatgcaggc tatttggata aagatttgga aaaaatcgtc
     5701 ggtctacaga cagacaaacc tttgaaacaa gcattcatgc cgttcggcgg aatcaatatg
     5761 gctaacaatg cgctaaaaag caatggctat acaccaaacg atgaattgac acatattttc
     5821 actgactggc gcaaaacaca taatcaaggt gtatttgacg cttatactgc tgaaatgcgt
     5881 gctgctcgta aaaacaaaat cattacaggg cttccagacg cttacggtcg cggacgtatc
     5941 atcggtgact atcgtcgtgt ggcattgtat ggaatcgact atttgatgga acaaaagaaa
     6001 aaagatcatg atatgactgg ttatcataca atgagtgaag aagtgattcg tttacgtgaa
     6061 gaaatcactg aacaatatcg tgcattagcg caaatgaaag aaatggctgc gaaatatggc
     6121 tatgatattt ctaaaccagc tgcaaatgca aaagaagctg tccaatggct atacttcgga
     6181 tatttagcag caatcaaatc acaaaatggt gctgcgatgt caatcggacg tatctcagca
     6241 ttccttgata tctatatcca acgtgatttg gataatggag taatcacaga aaaagaagca
     6301 caagaattga tcgaccactt agtaatgaaa ttacgtatgg tcaaattcgc gcgtacacct
     6361 gaatataacc aattattctc aggattccct atctgggcta ctttatccat tgcaggtatg
     6421 ggattagatg gtcgttcatt agttacaaag aatgatttcc gtctgcttca tacattaaca
     6481 aatatgggac cttcaccaga accaaacttg actgttttgt attctgaaca ccttccagaa
     6541 ggattcagaa catatgctag cagaatcgct atcgaaagtt cttcgattca attcgaaaat
     6601 gataatcagc ttcgtgaaca atggggttct gatgattgtg cgattgcttg ctgtgtatca
     6661 ggaacagttg tcggaaaaga catgcaattc tttggcgctc gtgctaactt ggctaaaaca
     6721 ctattatatg ccatcaatgg cggagtcgat gaagtgactg gcgcccaagt tggaccagaa
     6781 tatcgtccaa tcacatcaga aatactagac tttgaagaag tgaaacataa cttcatgaat
     6841 atgatggatt ggttagcaga actatatgtc aatacgttga acatcattca ttatatgcat
     6901 gataaatatt cttatgaagc agctcagctg gctttgatgg attctgactt gcaacgcaca
     6961 tttgctacag gtattgctgg gatctctcat gcggcagaca gcttgtcagc gatcaaatat
     7021 gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag ttgattttga agtggatggc
     7081 gacttcccta aatacggaaa tgatgatgac cgtgcagatg aattagtttc atggatcttg
     7141 gaatacttca tgggacaaat caaacgtcat aaaacttacc gcaactcaaa accaacaact
     7201 tcattattga cgattacttc aaatgttgtt tacggtaaaa atacaggaaa tactcctgat
     7261 ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc caagttacaa tgcggaacaa
     7321 agtgggttat tagctagcct gaactcaaca gctaaaatga attacggctg tgcattggat
     7381 ggaatttcaa atacccaaac aatcaatcct tcaggtcttg gaaaagaaca agatacaaga
     7441 atcgaaaact tgcgtaatgt cttagatgga tacttctcaa aaggagctta tcatttgaat
     7501 gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg aaaatccaat gaaatatccg
     7561 aacttgacga tccgggtatc tggttatgca gttaaattcc gtgacttgac aagagaacaa
     7621 caattagatg tcttgatgag aacttcacac gacagaatgt aaaaagttcg tgaaaaaagt
     7681 ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc atcaaaatcg aataatcagt
     7741 atgcttattt gaaagaaacg atttacaggg gcaggacttg tatgttccgt ccctgtaaat
     7801 tttacacata acgaagcggc aattgcgaaa aattttgttc agaaagcttc gcttcgacag
     7861 ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa cttaaatgga agaaaaaaca
     7921 atcggctatg ttcattctat cgaaactttc ggttcagtag acggacctgg actgcgcttt
     7981 gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt gtcacaatcc agatacttgg
     8041 aatattggcg gagggaaaga atacacagca gatgaacttt tagataaagc agaacgcttc
     8101 agaccttact ggggagataa aggtggtatt actgttagtg gtggagaacc tctattgcag
     8161 atcgattttt tgatcgaact atttaaaaaa gcaaaagagc ggaagatgca tactacattg
     8221 gatacatgcg gaaaaccttt tacgtatgaa gaacctttct tttcacgttt tcaagaattg
     8281 atgaagtata cagatttact gttatttgac atcaaacaca tcgataatga agagcataaa
     8341 aaactaacgc attgggacaa tgaaaatatt ttagaaatgg ctcaatattt gtcaaaaatc
     8401 aacaaaccgg tttggattcg ccatgtacta gttcctgagc gaagtgatta tgatgaatat
     8461 ttgatccgac tagacaactt tattcaaacc ttgtcaaatg tagacagagt agagattctt
     8521 ccttatcata cgatgggaaa atacaagtgg gaaacacttg gtttgaaata tccattagaa
     8581 gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta aacgattgct tcatacagaa
     8641 gagtatacaa aatatttaac gcgataggtc gcatcggaaa gaagataagt attgcttctt
     8701 tccgtttttt tgctattatt aaataggaga aatcacaaag gagcgaatga catgtcaaaa
     8761 attttagttt ttggtcacca aaatccagat acagatgcaa tcggggcagc cattgcgttt
     8821 tcacacttgc aaaatgaatt aggtaaagaa acagaagcag ttgctctagg tacacctaat
     8881 gaagaaacac aatacgcatt agaccacttt cagctatctg caccacgagt agtagagtca
     8941 gctaaagcag aaaatgcaga tgtcatgctt gtggatcata atgaattcca acaaagtatt
     9001 tcagatatca gcgaagtaaa tattttagct gtagtagacc accatcgtat tgcaaacttt
     9061 gaaacagcta atccattgta ctatcgtgca gaaccagttg gatgtacaag tacgattatt
     9121 ttaaaactat ataaagaaaa ccaagtaaca attcctaaag aaatcgcagg aatcatgctc
     9181 tcagctatca tttcagatac attgttgttc aaatcaccaa catgtacgcc tgaagatgaa
     9241 gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc taaacactta tggacttgaa
     9301 atgttgaaag caggaacaaa tctaagtgat aaatcagctg cggttcttct tgatttggat
     9361 gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg ctcaagtcaa tacagtggac
     9421 cttaatgaag tgatggatcg acaagcagag ctagaagcag caatggaaga tgaaatgaaa
     9481 gcaaatggct acgatctgtt cgtattagtt gtcacaaaca tcttagacag tgattcagaa
     9541 ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag ctttccaaac aactttgacc
     9601 aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa aacaagtcgt acctcaattg
     9661 acggctgtat ttagctaatc aaatggtagt atgaagcatt caaaatgaat ggataagcga
     9721 ttgatataaa aagaaacata gaacatacta aaggccgaag aaatggagga gaataacagt
     9781 caattatgac tcttgttctt cctccttttt acgtaaaagc cttttaagta aaataaaacg
     9841 gatagaaaag agaacatata tgaactatta ttcaaaaaat aaggaaaact tttatcaaaa
     9901 actgaccgat gatccgcttt tttcttcatt gaccgattat ctatatgaac atcgagaaca
     9961 agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa aataaatttt cccattttct
    10021 tgatttattg atcgatgcag gattaatcaa gagggaagaa cgaagatatc atttgaactt
    10081 tccaatattt gatcctaaag attacttgca acaagccact tcggctgctg aaaccatagc
    10141 tgaacaattg aagagactct ctgttgatga acaaaaatta gcgatgggag aagttatttg
    10201 ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt tatggagtaa gaaacagccg
    10261 agagacagaa ttgctgagga caactgcagg aaatgagaag taccgtttta tcacactttc
    10321 ctctatagaa cacttcccgc ttactttggc taattacttt tttgtacaga agaatcagct
    10381 tcctgtaacg aaggcattca aagaattagc cgaacttatt ggagacgtaa atgaatcgta
    10441 cttttttgat caaatagaag ttatcgttga caggatacgt aagaataaat ataaaaatcg
    10501 ccggcctagt attttccatc agtctctact tgtgacgaat actataaagg aagaagaatc
    10561 attcacatta gaattaccta ttgtcgaaaa aaacaatttt gagattgaat taccaacatt
    10621 ggatccttca ttgacaatgg aggaaatggc ctttcttaaa cgacaaatct tttctgagct
    10681 gagcaagaaa tttataccgc atgcattttc ttatatcaaa gaatatggaa cagttttagt
    10741 gagtaaaacc taagagagca gtaagcaaaa attatactga ctcttgcagg aggagccttt
    10801 ctcttccttt atggttaaaa gaattatttg agtattttgc ctatctacca cctattagat
    10861 tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg tgagaaatgt ggaatgacaa
    10921 aggaagtagt gataagaaaa gttagatttt taaataatca atattatgat tcagtcaagt
    10981 atggcatttt atgggaagaa ctagctgatt agattaggaa gtccatttga taatccagaa
    11041 tcactaagaa acgaatggcg agtcattaat aatttatcca atcttgggtt attcttttag
    11101 attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt aatagtcagg cttcaaaatg
    11161 ctttttattt tgtttgtttt ttctttgcca cataaataca cctagactga cgatgaagag
    11221 catatccaac agatttcctg cgacttggat cgtcgaaggg ttggtcagtg gcattaaagc
    11281 aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg acaggaacta atcgaaagaa
    11341 gagcggacat ttcaattggc taaaaatgag tgaaaaaata aaataagtaa aaatggataa
    11401 aatcacgact aaaccattca tgataaagaa gaaaagcgta ttgtgattaa acgaataaaa
    11461 aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat acgcttgaag cttcgtttaa
    11521 cgtaccagga tgtcgatgaa aaaaataggc accgaaataa acagaaaaca gtaagccgca
    11581 aagagaagac aaaatcaaaa aaaacggatt ttcttcggct tctgtgatat cagaatgatc
    11641 caaaagcaac cagcctaatg cactagaagg cgcatgtgag gtcttaaggt atgaataaca
    11701 tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct aaatgtaaaa aattagtggt
    11761 tctacgttga atttccataa tatctccaaa ttaaattgat
//
LOCUS       DAZAOG010000075        11667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000075 DAZAOG010000000
VERSION     DAZAOG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.075"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACHDLD_001724"
     CDS             complement(37..216)
                     /locus_tag="ACHDLD_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363418.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACHDLD_001725"
     CDS             complement(232..432)
                     /locus_tag="ACHDLD_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363419.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACHDLD_001726"
     CDS             complement(597..1304)
                     /locus_tag="ACHDLD_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363420.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACHDLD_001727"
     CDS             complement(1297..1734)
                     /locus_tag="ACHDLD_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363421.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACHDLD_001728"
     CDS             complement(1749..2000)
                     /locus_tag="ACHDLD_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363422.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACHDLD_001729"
     CDS             complement(2000..2206)
                     /locus_tag="ACHDLD_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN3363423.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACHDLD_001730"
     CDS             complement(2643..2903)
                     /locus_tag="ACHDLD_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363424.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACHDLD_001731"
     CDS             3077..3181
                     /locus_tag="ACHDLD_001731"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN3363425.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHDLD_001732"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHDLD_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3363426.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACHDLD_001733"
     CDS             complement(3810..4709)
                     /locus_tag="ACHDLD_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN3363427.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACHDLD_001734"
     CDS             complement(4722..5315)
                     /locus_tag="ACHDLD_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN3363428.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACHDLD_001735"
     CDS             complement(5391..5540)
                     /locus_tag="ACHDLD_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363429.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACHDLD_001736"
     CDS             complement(5556..7760)
                     /locus_tag="ACHDLD_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN3363430.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACHDLD_001737"
     CDS             complement(7771..9231)
                     /locus_tag="ACHDLD_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN3363431.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACHDLD_001738"
     CDS             complement(9292..11664)
                     /locus_tag="ACHDLD_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN3363432.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DAZAOG010000076        11586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000076 DAZAOG010000000
VERSION     DAZAOG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.076"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHDLD_001739"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHDLD_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN3363433.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHDLD_001740"
     CDS             complement(322..891)
                     /locus_tag="ACHDLD_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN3363434.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHDLD_001741"
     CDS             1077..1979
                     /locus_tag="ACHDLD_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3363435.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHDLD_001742"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHDLD_001742"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN3363436.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHDLD_001743"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHDLD_001743"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN3363437.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHDLD_001744"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHDLD_001744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN3363438.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHDLD_001745"
     CDS             8547..8732
                     /locus_tag="ACHDLD_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN3363439.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHDLD_001746"
     CDS             complement(8794..9990)
                     /locus_tag="ACHDLD_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN3363440.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..>11586)
                     /locus_tag="ACHDLD_001747"
     CDS             complement(10160..>11586)
                     /locus_tag="ACHDLD_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN3363441.1"
                     /translation="FGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGMPFQA
                     VFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAMVFIC
                     GLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQSAISS
                     SVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIGIVAT
                     TVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQVLMT
                     IIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIATSVG
                     AIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATAPALI
                     LVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVIKGKY
                     NQVSVALWIVDILFIINFIILATI"
BASE COUNT         3414 a         2474 c         2038 g         3660 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaa
//
LOCUS       DAZAOG010000077        11552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000077 DAZAOG010000000
VERSION     DAZAOG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.077"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..637)
                     /locus_tag="ACHDLD_001748"
     CDS             complement(113..637)
                     /locus_tag="ACHDLD_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN3363442.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="ACHDLD_001749"
     CDS             complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="ACHDLD_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN3363443.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1226..2206)
                     /gene="pta"
                     /locus_tag="ACHDLD_001750"
     CDS             complement(1226..2206)
                     /gene="pta"
                     /locus_tag="ACHDLD_001750"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN3363444.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2359..3039)
                     /locus_tag="ACHDLD_001751"
     CDS             complement(2359..3039)
                     /locus_tag="ACHDLD_001751"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN3363445.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3165..4034
                     /locus_tag="ACHDLD_001752"
     CDS             3165..4034
                     /locus_tag="ACHDLD_001752"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3363446.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4060..4575
                     /locus_tag="ACHDLD_001753"
     CDS             4060..4575
                     /locus_tag="ACHDLD_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN3363447.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4622..5185)
                     /locus_tag="ACHDLD_001754"
     CDS             complement(4622..5185)
                     /locus_tag="ACHDLD_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363448.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5240..5419)
                     /locus_tag="ACHDLD_001755"
     CDS             complement(5240..5419)
                     /locus_tag="ACHDLD_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363449.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5496..6005)
                     /locus_tag="ACHDLD_001756"
     CDS             complement(5496..6005)
                     /locus_tag="ACHDLD_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363450.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6101..6481)
                     /locus_tag="ACHDLD_001757"
     CDS             complement(6101..6481)
                     /locus_tag="ACHDLD_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3363451.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6811..7560
                     /gene="pnuC"
                     /locus_tag="ACHDLD_001758"
     CDS             6811..7560
                     /gene="pnuC"
                     /locus_tag="ACHDLD_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN3363452.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7744..9021)
                     /locus_tag="ACHDLD_001759"
     CDS             complement(7744..9021)
                     /locus_tag="ACHDLD_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363453.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9100..9744)
                     /gene="pcp"
                     /locus_tag="ACHDLD_001760"
     CDS             complement(9100..9744)
                     /gene="pcp"
                     /locus_tag="ACHDLD_001760"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN3363454.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9943..10887)
                     /locus_tag="ACHDLD_001761"
     CDS             complement(9943..10887)
                     /locus_tag="ACHDLD_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN3363455.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10889..>11552)
                     /locus_tag="ACHDLD_001762"
     CDS             complement(10889..>11552)
                     /locus_tag="ACHDLD_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN3363456.1"
                     /translation="GVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTS
                     FVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAF
                     NVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFI
                     GGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGK
                     GQ"
BASE COUNT         3282 a         2456 c         2041 g         3773 t
ORIGIN      
        1 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
       61 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
      121 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
      181 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
      241 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
      301 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
      361 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
      421 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
      481 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
      541 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
      601 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
      661 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
      721 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
      781 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
      841 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
      901 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
      961 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     1021 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     1081 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     1141 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     1201 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     1261 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     1321 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     1381 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     1441 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     1501 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     1561 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     1621 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     1681 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     1741 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     1801 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     1861 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     1921 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     1981 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     2041 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     2101 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     2161 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     2221 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     2281 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     2341 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     2401 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     2461 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     2521 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     2581 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     2641 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     2701 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     2761 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     2821 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     2881 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     2941 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     3001 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     3061 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     3121 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     3181 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     3241 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     3301 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     3361 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     3421 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     3481 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     3541 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     3601 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     3661 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     3721 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     3781 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     3841 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     3901 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     3961 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     4021 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     4081 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     4141 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     4201 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     4261 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     4321 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     4381 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     4441 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     4501 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     4561 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     4621 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     4681 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     4741 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     4801 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     4861 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     4921 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     4981 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     5041 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     5101 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     5161 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     5221 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     5281 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     5341 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     5401 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     5461 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     5521 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     5581 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     5641 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     5701 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     5761 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     5821 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     5881 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     5941 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     6001 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     6061 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     6121 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
     6181 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
     6241 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
     6301 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
     6361 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
     6421 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
     6481 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
     6541 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
     6601 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
     6661 aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa
     6721 agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac
     6781 tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat
     6841 cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct tggctgccag
     6901 gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc
     6961 acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta
     7021 ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt
     7081 atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa
     7141 tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg gtttatcgca
     7201 atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat
     7261 gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc
     7321 agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg
     7381 gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc
     7441 tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa
     7501 gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa
     7561 aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     7621 aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
     7681 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctctat
     7741 tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc
     7801 taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc
     7861 tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg
     7921 ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc
     7981 tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc
     8041 ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat
     8101 gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg
     8161 ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac
     8221 ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa
     8281 ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg
     8341 tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc
     8401 ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc
     8461 taattttata tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca
     8521 gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc
     8581 gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga
     8641 gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg
     8701 cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg
     8761 gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc
     8821 agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac
     8881 tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta
     8941 ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa
     9001 gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac
     9061 taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt
     9121 tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc
     9181 aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga
     9241 gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag
     9301 caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg
     9361 tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc
     9421 ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat
     9481 tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc
     9541 ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg
     9601 actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc
     9661 atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc
     9721 aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt
     9781 tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata
     9841 acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag
     9901 aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat
     9961 ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc
    10021 gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg
    10081 tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt
    10141 cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc
    10201 aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc
    10261 gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac
    10321 tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg
    10381 ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg
    10441 agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc
    10501 acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat
    10561 aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg
    10621 gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc
    10681 accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca
    10741 gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt
    10801 gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa
    10861 aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg
    10921 ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca
    10981 ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg
    11041 ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc
    11101 agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt
    11161 ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc
    11221 cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa
    11281 tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt
    11341 ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca
    11401 tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac
    11461 cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa
    11521 atgcaaaccc gagaataaca atcaagacgc ct
//
LOCUS       DAZAOG010000078        11520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000078 DAZAOG010000000
VERSION     DAZAOG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.078"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(216..701)
                     /locus_tag="ACHDLD_001763"
     CDS             complement(216..701)
                     /locus_tag="ACHDLD_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN3363457.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    791..1136
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1181..1822
                     /locus_tag="ACHDLD_001764"
     CDS             1181..1822
                     /locus_tag="ACHDLD_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3363458.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1841..2476
                     /locus_tag="ACHDLD_001765"
     CDS             1841..2476
                     /locus_tag="ACHDLD_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3363459.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2494..3309
                     /locus_tag="ACHDLD_001766"
     CDS             2494..3309
                     /locus_tag="ACHDLD_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3363460.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3628..5064
                     /locus_tag="ACHDLD_001767"
     CDS             3628..5064
                     /locus_tag="ACHDLD_001767"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3363461.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5253..7700
                     /locus_tag="ACHDLD_001768"
     CDS             5253..7700
                     /locus_tag="ACHDLD_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN3363462.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7930..8997
                     /gene="rlmN"
                     /locus_tag="ACHDLD_001769"
     CDS             7930..8997
                     /gene="rlmN"
                     /locus_tag="ACHDLD_001769"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN3363463.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8987..9193
                     /locus_tag="ACHDLD_001770"
     CDS             8987..9193
                     /locus_tag="ACHDLD_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363464.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9190..10293)
                     /locus_tag="ACHDLD_001771"
     CDS             complement(9190..10293)
                     /locus_tag="ACHDLD_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3363465.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10846..11394
                     /gene="aac(6')"
                     /locus_tag="ACHDLD_001772"
     CDS             10846..11394
                     /gene="aac(6')"
                     /locus_tag="ACHDLD_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN3363466.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3825 a         1946 c         2483 g         3266 t
ORIGIN      
        1 tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga atcaataaat
       61 atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca tactagttgt
      121 tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga
      181 cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata aactggcaga
      241 tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta
      301 ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact
      361 tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg
      421 cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt
      481 tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt
      541 tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg
      601 acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt
      661 tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt
      721 tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt
      781 aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc
      841 tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa
      901 gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga
      961 taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc
     1021 tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt
     1081 tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat
     1141 ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt
     1201 tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat
     1261 tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct
     1321 acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact
     1381 gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc
     1441 actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat
     1501 acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca
     1561 aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa
     1621 tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt
     1681 aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt
     1741 ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac aagttgaagg
     1801 aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt
     1861 gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg
     1921 gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt
     1981 ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc
     2041 attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt
     2101 tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac
     2161 aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt
     2221 attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag
     2281 agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc
     2341 tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca
     2401 aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt
     2461 ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct
     2521 ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat
     2581 tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct
     2641 atgggattta gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca
     2701 gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa
     2761 tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca
     2821 agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca
     2881 cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa
     2941 aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc
     3001 aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc
     3061 agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga
     3121 aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt
     3181 ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta
     3241 gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg
     3301 agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa
     3361 acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt
     3421 ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag
     3481 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
     3541 ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat
     3601 tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa
     3661 gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat
     3721 gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga
     3781 acgaacggtg atttggcagt agaccactat catcgttata aagaagatat tgctttaatg
     3841 aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat
     3901 ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg
     3961 attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg
     4021 caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca
     4081 gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa
     4141 aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg
     4201 aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa
     4261 tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat
     4321 ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc
     4381 aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta
     4441 gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct
     4501 aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta
     4561 aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact
     4621 ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac
     4681 tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc
     4741 atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa
     4801 caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa
     4861 gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt
     4921 agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca
     4981 caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt
     5041 gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat
     5101 ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga
     5161 aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt
     5221 ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa
     5281 aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga
     5341 ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt
     5401 taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct
     5461 gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg
     5521 gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac
     5581 atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg
     5641 atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga
     5701 ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga agttatttag
     5761 cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc
     5821 tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg
     5881 ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga
     5941 aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga
     6001 agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa
     6061 atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc
     6121 ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt
     6181 tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac
     6241 aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg
     6301 atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc
     6361 aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat
     6421 tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata
     6481 tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac
     6541 tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg
     6601 aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg
     6661 gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg
     6721 gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc
     6781 cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg
     6841 gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac
     6901 aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa
     6961 atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc
     7021 caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca
     7081 gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc
     7141 atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa
     7201 atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg
     7261 attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg
     7321 agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg
     7381 ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg
     7441 aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag
     7501 cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg
     7561 gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt
     7621 cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc
     7681 cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa
     7741 aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg
     7801 gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga
     7861 agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg
     7921 gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg
     7981 tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa
     8041 aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta
     8101 tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt
     8161 acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga
     8221 caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg
     8281 ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca
     8341 cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat
     8401 gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg
     8461 catatcatca atgatgccaa aggattggca attggtgctc ggcacattac agtttccact
     8521 agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct
     8581 atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt
     8641 ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga
     8701 atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa
     8761 ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat
     8821 ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat
     8881 gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt
     8941 gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg
     9001 tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt
     9061 caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa
     9121 aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt
     9181 agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt
     9241 ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt
     9301 aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag
     9361 attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc
     9421 aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc
     9481 tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc
     9541 catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat
     9601 atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc
     9661 ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct
     9721 atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa
     9781 agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc
     9841 aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc
     9901 gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt
     9961 gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc
    10021 accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat
    10081 atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt
    10141 caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac
    10201 tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat
    10261 ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa
    10321 cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa
    10381 cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta
    10441 tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct
    10501 tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag
    10561 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
    10621 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
    10681 ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag taagataaat
    10741 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
    10801 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
    10861 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
    10921 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
    10981 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
    11041 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
    11101 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
    11161 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
    11221 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
    11281 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
    11341 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
    11401 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc
    11461 ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac
//
LOCUS       DAZAOG010000079        11432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000079 DAZAOG010000000
VERSION     DAZAOG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.079"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..579)
                     /locus_tag="ACHDLD_001773"
     CDS             complement(34..579)
                     /locus_tag="ACHDLD_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN3363467.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(591..1283)
                     /locus_tag="ACHDLD_001774"
     CDS             complement(591..1283)
                     /locus_tag="ACHDLD_001774"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN3363468.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1302..1586)
                     /gene="macP"
                     /locus_tag="ACHDLD_001775"
     CDS             complement(1302..1586)
                     /gene="macP"
                     /locus_tag="ACHDLD_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN3363469.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1599..2162)
                     /locus_tag="ACHDLD_001776"
     CDS             complement(1599..2162)
                     /locus_tag="ACHDLD_001776"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN3363470.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2390..3052
                     /locus_tag="ACHDLD_001777"
     CDS             2390..3052
                     /locus_tag="ACHDLD_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN3363471.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3176..4372
                     /locus_tag="ACHDLD_001778"
     CDS             3176..4372
                     /locus_tag="ACHDLD_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN3363472.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4473..5849)
                     /locus_tag="ACHDLD_001779"
     CDS             complement(4473..5849)
                     /locus_tag="ACHDLD_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3363473.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5886..6752)
                     /locus_tag="ACHDLD_001780"
     CDS             complement(5886..6752)
                     /locus_tag="ACHDLD_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN3363474.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6841..7866)
                     /locus_tag="ACHDLD_001781"
     CDS             complement(6841..7866)
                     /locus_tag="ACHDLD_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3363475.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7884..8558)
                     /locus_tag="ACHDLD_001782"
     CDS             complement(7884..8558)
                     /locus_tag="ACHDLD_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3363476.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8742..9635)
                     /locus_tag="ACHDLD_001783"
     CDS             complement(8742..9635)
                     /locus_tag="ACHDLD_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3363477.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9709..11406)
                     /locus_tag="ACHDLD_001784"
     CDS             complement(9709..11406)
                     /locus_tag="ACHDLD_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3363478.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3386 a         2515 c         1964 g         3567 t
ORIGIN      
        1 gtgcttttta ttttatcaaa tcactttgtt tttttataaa atttctgcgc ctagtgtgga
       61 ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg aaacttgcca cggcatcttt
      121 gaaaatatgg agtttgtatc ctagattata cgcatcgact gctgtatgca acacacagat
      181 atctgtacat acgccggtca aataaatatc tgtgatctgg cgttctcgca aacggatgtc
      241 tagatctgtt ccactaaaag cggaataatg ccgtttgtct atccaataaa cattttcctc
      301 ttgctcatag tcttggtata aactttttaa agagccgtac aattcccgtc cacccgttcc
      361 tatcacatta tgcggaggaa ataagcggtt ctccggatga aaggaatctt ttggatcatg
      421 cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata aacttctttg tatgagcagt
      481 cagctttttt tcaattactt gtcctgctgt cccagtagtc agctttccat cctctgcaac
      541 aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga
      601 tgtttgacaa attcgatcac catttctgct gatcgtttgc ctgcgtcttc gataaattcg
      661 tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat
      721 gggatattga actgacgagc tgtttgtcca acagcggctc cttccatttc gcaagctaat
      781 gcttcaggga aattcgttaa gatttctttg attttatctg gcgagtcgac aaatgtatca
      841 cctgtaacga tcaatccttc ttttgcgttc aaattcgttg cttttgctgc tttaaccatt
      901 tcactgcgca aatatgtgct tgcttcataa tatagaggca ttcctggtaa ttgtcctggt
      961 ttgtagccaa atcctgttgc atctgcatca aaataagcta ctttgtctga tataacgatg
     1021 tcacctactt gtaagccttc accgattccg ccagctgaac ctgtattgat caccatgttc
     1081 acgccgtatt gttggatcaa cagacttgtg gtaatagatg ctaagacttt tcctataccg
     1141 gaacgtacga cgatcacttc gtggttacct aatgaacctg aaataaataa agcgcctgcg
     1201 cgttcccatg ataatggttc gcttaggttc tctctcaaaa tttttacttc ttcttccatt
     1261 gcgccgataa taccgatttt catcttttta attccttctt tctatataaa tgcgattgct
     1321 aaaaaaacca cacataacat taggataaca ataataagag atttcatcaa aaaagagttc
     1381 catttggtcg tcttttcccg ttcgctgatc cttgttttcg tgattggttt attctttttg
     1441 ctttctttac gataaaagct ggcaatcttt ttttcttctt gctgataagc tgtttctgct
     1501 tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt
     1561 cgcgtaataa gcggtccttt ggccataagc cgatccctct actttccttt ggtcaatagt
     1621 tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct
     1681 tgttgcgcct cttcaagagt tagatgatac aactccagta cttcgtcttc atcttgtgct
     1741 aaaggattct cgaccttctc taccccttgt gcatgataaa tatgaaggac ttcatttgca
     1801 aaacctggtg aaagatacat cgaagtaaga tggctcagcg acttcgcacg atagccagtt
     1861 tcttcttcta attctctagc tgctgtcatt tcgggatttt gtccttctcc tggatcgatt
     1921 tttccagctg gaatctccaa aatcactttt tctaaaggtt ttcgaaattg tttgacaaga
     1981 agcagacgat cttgttcgtc aaaagcaatg atcccgacac caccaggatg aaataccagt
     2041 tctctcttcg aagttcctcc atctggcaaa cgaacttcgt ctacagcaac atcaataatt
     2101 tttccgctat agatttcttt gcgactgatt gttttttctt caaattgctc attgttcagc
     2161 atatcttctc ctgctttctt tgtaatcata actgcttttt tttcattttt caatttttct
     2221 caaaaactcc ttattagttt accatagata aaccttagat tttctctaaa tatagaaaat
     2281 ccttctttag tccgatactc tttctagtag tccgtatgct aaaataagta tgtaatcgaa
     2341 taacgaaaag aattcaatac tcaacgataa atgacatgga gggtttatta tgataaaacg
     2401 aaattggccg tggcttctag cattggtctt gctatttctc tttgtccgaa acaatagttt
     2461 ttcttttgtt ctaggacttt tattgatagg tgtagtttta gttttgtggg gactctttgg
     2521 ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca ctgactgacg aacttgaaac
     2581 tcactatgaa aaaagcggta tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa
     2641 tcaaacaaaa aatgagatca atcaattgca aatgaatatg cagcagacag ccaaacttaa
     2701 agcaatcgat ctccgccatg atccagtcaa agcagcgaaa gctttattca aagaattggt
     2761 gaaagaacca aaccgactgc atgaagcaag tcagtttctt tatacgcatc tgccgaatct
     2821 agttgatttg acaaataagt ataatgagat caatgaccat gagatcaaaa acaaacagac
     2881 ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt taatcgcaca
     2941 agattatcaa aaattcgttg ctgaggatct agatgatatc gacgtagaga tatctgttgc
     3001 gaaacagagc ttaaagcgtg ataataaata tgaaccagaa caaaaggatt aactcttttg
     3061 ttcataaaag aagactcgga acattcagat gatgtctggc tgtttcgagt actttttcta
     3121 aagtagagac attcatgtaa gaatcgagaa cagattcaac aaggaggaat tttttatgga
     3181 tcataaaccg actgaaacaa aagatgttac accagttaac gatacacttg aagatttatt
     3241 gaacaacccg ttttcaactc caacagatac gctgaccact tcacaacaag cggagattga
     3301 tcagctccaa gagcaacaga cagctgctcg tttaattgac aaactaccgg cggaacgcca
     3361 agaacaagct aaacagctag cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag
     3421 ctacggatcg gctgcccaag caaaattaag tgaattctca caatcgatgt tgaatcatgt
     3481 gcaagcacaa gatattggtc ctgtaggaga ttccttaact gaactgatgt atcgtctcca
     3541 agaagccaat cctgacgaac tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg
     3601 gaaggtcaaa caatcgatct atgaagtgac agcaaaatat caaaagattg gtgcacaaat
     3661 tgacaagatt tcagtgaaat tagataaaga aaaagacggt ctgctaaaag ataatctgat
     3721 gttagaacag ctttaccaga aaaacaaaga ctactttgat gcactgaata tttatatcgc
     3781 tgctggagaa ttgaaaatgg aagaacttca aacaacgatc attccagaag cgatgaaaag
     3841 agcagaagaa actggcgatc agatggatgt tcagattgcc aatgattaca cgcaattcct
     3901 agatcgtctg gataaacgta cgcatgatct tcgcttagct agacagatca cgatccagca
     3961 agcaccacaa atccgtttga tccaaaacac aaatcaggcc ttggctgaaa agatccaagc
     4021 atctatcgct acagccattc cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct
     4081 tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa acgacttatt
     4141 gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg
     4201 tggaatcgtg gatatcgaga cccttcaaac cactcaaaat gatttgatcg aaacaatcca
     4261 agagacattg cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct
     4321 tggacacatg gaagaagatc tgaaacaaaa actgttggat ttaacccaat agatcacctt
     4381 tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta caactgttga tgggcgtttt
     4441 tcttatcttt cttctaactt ctccattgga gtctatttct cattcatctt gctgtgtcca
     4501 tagccatagc caaaataaat gatcagtcca ataaccatta cgataccgaa cacgatccat
     4561 gtcacagttt gcagacgaag catcaataca atacaagtca agatggagac gattggcaat
     4621 agcggaacga atggcacttt gaattctcca gcttttggtt tgccaaacat ttttctcaac
     4681 ttcagtaagc ctagactaac aagcatcaaa taggcgagcg ccatgatatt cgttagttcc
     4741 gctaatacat tcaatgggaa aaatccggca aagaatacag ctgccacacc agcggcataa
     4801 gtcgcttttt tcggtgtacg atgcttttga tcgatttctg tcataaactt aggaagtaag
     4861 ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat acatcatcga gatcgttacc
     4921 gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc atgtcctaca
     4981 tacctcattg caaaagccac tgggtcattg actcctagtt cagtatacgg aacgattcct
     5041 gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga accaatgatt
     5101 cctcttggca catctttttg cgggtttttg acttcctcgg ctgccattgc aacggcatca
     5161 aatcccaaga aagcaaaaaa cacaagagct gcaccatcta gcacaccgga aaaaccaaaa
     5221 ggcatgaatg gctgccagtt atcaggttta acgaaaaata ttcctacaat gataaacaac
     5281 gcgatgatac caaatttgac atataccatg gcattattca aacgtaatgc tttcttggct
     5341 tcaagcgaca cgatgtaagt gacgaatatc actacgagca ccgcaatcaa gtcaatgtat
     5401 gtaccatttt ccggattgta cccgccagtt aatgcttgtg gaagttcggc ccctaaactt
     5461 ttcaaaaaac cttgcatata gccagaccat ccggaagcaa cagaggaaac cgctagtaaa
     5521 aattccccga tcaacaacca ccctgtcagc caggcagcaa attcaccaaa tacaacatac
     5581 agataggcat atggcccacc aatcactggc actcgcgaag caaattctgc gaaacttagt
     5641 ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc tggaccagca
     5701 ttttgattgg ccgcaacccc tgtcaccaca aatattcccg taccgatgat cgcaccaatt
     5761 ccaagcatga tcaaatcaga cgtttttagt tcttttttca tctcactcgt atgattgaga
     5821 ttgatctctt tcttacgaaa caaatccatt gaagttcctc cccattttag gttggaaaac
     5881 agcgattaaa ctgttttcat ataccctaaa aggataccac ctaaaatgac taaaaggcaa
     5941 ccaacaataa cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca
     6001 cctaaagtag agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt
     6061 gatttgactg ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg
     6121 tttttccaca caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag
     6181 atcattccga tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg
     6241 atgatggtat atacaccata accaatcgta gagaagatca atgctctaaa acccttacca
     6301 aaatcaagca ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa
     6361 tacgcaccgc ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt
     6421 gtccattcat ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta
     6481 gacaatggta gccctacaga aacacccatg tacttcattc catggaactg tccattttgt
     6541 ccaacactcc aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca
     6601 ggactaacaa cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt
     6661 gtttgttgat tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc
     6721 aacataggga ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata
     6781 agaaacttat cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt
     6841 ctaacgtttt tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt
     6901 tccttcagtt gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa
     6961 ataaagtcct aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc
     7021 cttatcaaag atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga
     7081 aaggctgact ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt
     7141 atatttgatg gcattactaa gcaattggtt gaagataaaa gcgatccatt tagcatcggt
     7201 caaaacagta tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt
     7261 tgcctgactg cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta
     7321 gtcccgcgag aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat
     7381 ttttgataat tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga
     7441 acgaactagc aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat
     7501 ataatctttt tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg
     7561 gatcagctga ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa
     7621 ataattttgc aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt
     7681 gctcaagtaa ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata
     7741 agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa
     7801 tgcaataaaa aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa
     7861 attcatctta accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg
     7921 atatatcctt ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc
     7981 aatgtattgt catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga
     8041 cttaagatct ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc
     8101 aaatcaatga tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca
     8161 ttgtggctca tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca
     8221 ttgatttttg caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc
     8281 atgttcatag ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt
     8341 actttcgata cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta
     8401 atatctagta atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa
     8461 tcggtcgttc caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga
     8521 atgatctctt cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt
     8581 cttgctacta caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc
     8641 attttagcaa acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca
     8701 agcagcttaa atttattatg ttattcttaa taattggtct attatctgtt atttttcttt
     8761 cgttgttttt gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc
     8821 aatataccta gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc
     8881 tttgaagaga cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg
     8941 ttcacggtgt aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct
     9001 aatgtatttg tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc
     9061 cgaccataca cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag
     9121 agttttttct cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca
     9181 gttggaaaag aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt
     9241 tcatcaaata aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt
     9301 tgttcgtaat attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct
     9361 ttgacttgtc ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca
     9421 tgcactgcta attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa
     9481 ttctcgatcc ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta
     9541 aaaactgcag ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt
     9601 tgggtttgag gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa
     9661 actgcagcaa cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga
     9721 cttcatttta gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag
     9781 taagaatgcc aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg
     9841 tactctttct ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc
     9901 ataagagatg ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg
     9961 cgccatggtc tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc
    10021 atttccatct gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc
    10081 tcctgattca taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg
    10141 gtcttcattc cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata
    10201 tttttcaaac tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata
    10261 atcaaattca tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc
    10321 ttctagttct gtggaaacct tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc
    10381 tgctaatcta cctaaagtat ctactttggc tactccattt tcttctgtca aaatcgtcac
    10441 gacaaacccg ttgcttacta cttctactat ataatcttgt ccttttgtta aaatcgtctc
    10501 gccggctcgc acttctattt cctgatcata ataagttaga ccaactgtag gcgtatctgt
    10561 gattttgtaa tttttgataa cagcgcttcc gttttcaagg acagaatcga tgaaataagg
    10621 aataggtgct gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa tataagaaat
    10681 cgtttcttgt tctccagctt cattaataat cgttacttgt ctaaggtcgc tttcctctac
    10741 attcatctgt ttatctgctt ctctgatttc gtttttcgga tacaaatgca catctgtatt
    10801 gtatgtgtct ttccatgtag aaccatccca cacgccatcc accatatcag gcatcatcac
    10861 tggcatcata acaaccattg gagacgcgat tctggaaata tgagctggcg aataagtttc
    10921 taagaaaaga tagactttat ctctagaaga aggtgcttct tccgtcactt ggacggtaga
    10981 gaataccgct tctccagctt cattcgttgt ttgtttgtca atacttgtca gtcgatattt
    11041 taaaaaccaa ctttctgtgt gccgtttttg aatccattca atcgcttcgg cagaattaag
    11101 accgtcttct cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat atacttccgt
    11161 tacgtcaaac aaatcaaaat cgactcctgg aatattttct tcaccaaaat ccgtagaaac
    11221 caatccgcta ttcggtcgtt cttccggaac agatgagaaa ccttttttgt gtaatgtaat
    11281 cgtcacttca cttggttttt gtgcgacttc ttcggctttg actagttttc cgttcccgaa
    11341 aatccctatt cccgcagtaa cgagtaacag tacactcatt cccgtgacaa tcattttttt
    11401 attcattttt ttctccccgt tcttttttta tt
//
LOCUS       DAZAOG010000080        11347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000080 DAZAOG010000000
VERSION     DAZAOG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.080"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHDLD_001785"
     CDS             6..305
                     /locus_tag="ACHDLD_001785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3363479.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHDLD_001786"
     CDS             378..1739
                     /locus_tag="ACHDLD_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN3363480.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHDLD_001787"
     CDS             1761..2021
                     /locus_tag="ACHDLD_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363481.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHDLD_001788"
     CDS             2035..2787
                     /locus_tag="ACHDLD_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN3363482.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHDLD_001789"
     CDS             complement(2881..3486)
                     /locus_tag="ACHDLD_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3363483.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHDLD_001790"
     CDS             complement(3539..4513)
                     /locus_tag="ACHDLD_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN3363484.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHDLD_001791"
     CDS             complement(4891..5610)
                     /locus_tag="ACHDLD_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3363485.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHDLD_001792"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHDLD_001792"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN3363486.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHDLD_001793"
     CDS             7161..8558
                     /locus_tag="ACHDLD_001793"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3363487.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHDLD_001794"
     CDS             8639..9079
                     /locus_tag="ACHDLD_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3363488.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHDLD_001795"
     CDS             9227..9553
                     /locus_tag="ACHDLD_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3363489.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHDLD_001796"
     CDS             9554..10303
                     /locus_tag="ACHDLD_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN3363490.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHDLD_001797"
     CDS             complement(10383..10838)
                     /locus_tag="ACHDLD_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3363491.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3600 a         1973 c         2317 g         3457 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattctt
//
LOCUS       DAZAOG010000081        11207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000081 DAZAOG010000000
VERSION     DAZAOG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.081"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="ACHDLD_001798"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="ACHDLD_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN3363492.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="ACHDLD_001799"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="ACHDLD_001799"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN3363493.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="ACHDLD_001800"
     CDS             complement(2159..2896)
                     /locus_tag="ACHDLD_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN3363494.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="ACHDLD_001801"
     CDS             complement(2987..3514)
                     /locus_tag="ACHDLD_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363495.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="ACHDLD_001802"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="ACHDLD_001802"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN3363496.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="ACHDLD_001803"
     CDS             complement(4391..4876)
                     /locus_tag="ACHDLD_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN3363497.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="ACHDLD_001804"
     CDS             complement(5029..5226)
                     /locus_tag="ACHDLD_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363498.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="ACHDLD_001805"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="ACHDLD_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN3363499.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="ACHDLD_001806"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="ACHDLD_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN3363500.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="ACHDLD_001807"
     CDS             9201..9413
                     /locus_tag="ACHDLD_001807"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN3363501.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..11094
                     /locus_tag="ACHDLD_001808"
     CDS             9415..11094
                     /locus_tag="ACHDLD_001808"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN3363502.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3244 a         2423 c         1970 g         3570 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
    10021 ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat cttttcaaaa
    10081 atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt gcagcaagca
    10141 gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag catggaaaat
    10201 gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac atttgtagat
    10261 gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac aggttctcaa
    10321 ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca aatccaaatc
    10381 cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac aacaagtgtc
    10441 aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg aaaaatcaat
    10501 aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct tcgtttgatg
    10561 aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt acatgcttct
    10621 ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa tggcgatgtt
    10681 ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg agatgtgtat
    10741 atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg tcacctgctt
    10801 tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa agaaatcttg
    10861 gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg agaaatgatc
    10921 aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg acctgactgt
    10981 tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt attcgaacgt
    11041 acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga ataaataaga
    11101 ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa acatgagaag
    11161 ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcata
//
LOCUS       DAZAOG010000082        10987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000082 DAZAOG010000000
VERSION     DAZAOG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.082"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACHDLD_001809"
     CDS             complement(247..759)
                     /locus_tag="ACHDLD_001809"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN3363503.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHDLD_001810"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHDLD_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN3363504.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACHDLD_001811"
     CDS             complement(3212..3655)
                     /locus_tag="ACHDLD_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN3363505.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACHDLD_001812"
     CDS             complement(3656..4444)
                     /locus_tag="ACHDLD_001812"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3363506.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHDLD_001813"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHDLD_001813"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN3363507.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACHDLD_001814"
     CDS             complement(5559..6146)
                     /locus_tag="ACHDLD_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN3363508.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHDLD_001815"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHDLD_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN3363509.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACHDLD_001816"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACHDLD_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN3363510.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACHDLD_001817"
     CDS             complement(9063..9998)
                     /locus_tag="ACHDLD_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3363511.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10184..>10987
                     /locus_tag="ACHDLD_001818"
     CDS             10184..>10987
                     /locus_tag="ACHDLD_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN3363512.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
BASE COUNT         3099 a         2463 c         1911 g         3514 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    10141 aattgtattt ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct
    10201 taccatagac gaactgacaa tgatagaatc atattatctt caacataata agccggttga
    10261 aatcgctaac cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa
    10321 gcaaggcaag actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg
    10381 tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga aagtcactct
    10441 tggttggacg cctgacgtca ttatcgggcg aaaagaaagg cctgtttcat gcggtatgag
    10501 aacactttat cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg
    10561 taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat
    10621 ccatgatcga cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga
    10681 tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa
    10741 agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa
    10801 tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa
    10861 agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga
    10921 ccctggaaca ccttctcaac gcccattaaa cgagaattct aacgggattc tgcgtcgtaa
    10981 tggactg
//
LOCUS       DAZAOG010000083        10922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000083 DAZAOG010000000
VERSION     DAZAOG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.083"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHDLD_001819"
     CDS             complement(<1..281)
                     /locus_tag="ACHDLD_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363513.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHDLD_001820"
     CDS             453..1541
                     /locus_tag="ACHDLD_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3363514.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHDLD_001821"
     CDS             1669..3045
                     /locus_tag="ACHDLD_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN3363515.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHDLD_001822"
     CDS             3446..5026
                     /locus_tag="ACHDLD_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN3363516.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHDLD_001823"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHDLD_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHDLD_001824"
     CDS             complement(5652..5972)
                     /locus_tag="ACHDLD_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN3363517.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHDLD_001825"
     CDS             complement(6120..8354)
                     /locus_tag="ACHDLD_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3363518.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHDLD_001826"
     CDS             8833..9099
                     /locus_tag="ACHDLD_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN3363519.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHDLD_001827"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHDLD_001827"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN3363520.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAZAOG010000084        10807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000084 DAZAOG010000000
VERSION     DAZAOG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.084"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACHDLD_001828"
     CDS             complement(25..162)
                     /locus_tag="ACHDLD_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN3363521.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHDLD_001829"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHDLD_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN3363522.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACHDLD_001830"
     CDS             complement(1599..1853)
                     /locus_tag="ACHDLD_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363523.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACHDLD_001831"
     CDS             complement(1878..3020)
                     /locus_tag="ACHDLD_001831"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFN3363524.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACHDLD_001832"
     CDS             complement(3080..4432)
                     /locus_tag="ACHDLD_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN3363525.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACHDLD_001833"
     CDS             complement(4503..4847)
                     /locus_tag="ACHDLD_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN3363526.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACHDLD_001834"
     CDS             complement(4857..5231)
                     /locus_tag="ACHDLD_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3363527.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACHDLD_001835"
     CDS             complement(5244..5558)
                     /locus_tag="ACHDLD_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3363528.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHDLD_001836"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHDLD_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACHDLD_001837"
     CDS             complement(8976..9638)
                     /locus_tag="ACHDLD_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363529.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACHDLD_001838"
     CDS             10255..10485
                     /locus_tag="ACHDLD_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363530.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="ACHDLD_001839"
     CDS             10491..10790
                     /locus_tag="ACHDLD_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3363531.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         2997 a         2384 c         1832 g         3594 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaac
//
LOCUS       DAZAOG010000085        10730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000085 DAZAOG010000000
VERSION     DAZAOG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.085"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..1811)
                     /gene="glmS"
                     /locus_tag="ACHDLD_001840"
     CDS             complement(6..1811)
                     /gene="glmS"
                     /locus_tag="ACHDLD_001840"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN3363532.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2220..2510
                     /locus_tag="ACHDLD_001841"
     CDS             2220..2510
                     /locus_tag="ACHDLD_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363533.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2523..3548
                     /gene="sppA"
                     /locus_tag="ACHDLD_001842"
     CDS             2523..3548
                     /gene="sppA"
                     /locus_tag="ACHDLD_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN3363534.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3551..4210
                     /locus_tag="ACHDLD_001843"
     CDS             3551..4210
                     /locus_tag="ACHDLD_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN3363535.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4367..5299
                     /locus_tag="ACHDLD_001844"
     CDS             4367..5299
                     /locus_tag="ACHDLD_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363536.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5453..6742
                     /gene="tig"
                     /locus_tag="ACHDLD_001845"
     CDS             5453..6742
                     /gene="tig"
                     /locus_tag="ACHDLD_001845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN3363537.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6989..8239
                     /gene="clpX"
                     /locus_tag="ACHDLD_001846"
     CDS             6989..8239
                     /gene="clpX"
                     /locus_tag="ACHDLD_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN3363538.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8322..8909
                     /gene="yihA"
                     /locus_tag="ACHDLD_001847"
     CDS             8322..8909
                     /gene="yihA"
                     /locus_tag="ACHDLD_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN3363539.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8983..9138)
                     /locus_tag="ACHDLD_001848"
     CDS             complement(8983..9138)
                     /locus_tag="ACHDLD_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN3363540.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            9274..10602
                     /locus_tag="ACHDLD_001849"
     CDS             9274..10602
                     /locus_tag="ACHDLD_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363541.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         3552 a         1860 c         2222 g         3096 t
ORIGIN      
        1 ttttattatt ctactgtcac tgatttcgct aaattacgtg gtttgtcaac gtcgaatccc
       61 cgtaatagcg ttgcgtaata tgcaagtaat tgagtcggta cgactgtggt tagagtagtt
      121 agataagggt ggacagttgg tagcaccagc tggtcacttt ccttttctaa tccctctgag
      181 acaataacta gtgttttagc gccacggctt tcgacttctt tcaggtttcc tcgagtatgc
      241 gcaccagtca cttcttcact gatgattccg atcactggtg ttccttcttc gatcaaagca
      301 atcgttccat gtttcaattc gccagcggca aaaccttctg cctggatata actgatttct
      361 ttcaatttca aagcagcttc aagagaaacg ttgtagtcag caccacgtcc aatgtaaaag
      421 gcatttcttg tatcgctgag atatacttct gccaactctt ctaaataatc tttttgggcg
      481 atgaccgatt ccatcgcatt agcaactaaa ctcaattcat gagtgatatc gaatacaaga
      541 gaagtttcaa attctttttt atcaccgata gctttggcta ataaagttag tacagctatt
      601 tgtgcagtat aagctttcgt tgatgctact gcgatttcgg gtccagcatg caatagcagt
      661 gtaaaggatg cttcccgcga aagagtagaa ccagcaacat ttgtaatcgt aagtgaaggg
      721 tagccgagac ggttgatttt taccagcact tggcggctgt ctgctgtttc gccactttga
      781 ctcaagaaaa taaagaatgg tttttttgaa agaagaggtg tgttatagcc aaattcactg
      841 gctaaatgaa cctcaacagg aatctgtgtc aatttttcca aagtctgttt acctgctaac
      901 cccgcatggt aactagttcc gcaagcaacg atatagattc gatcgctatc agacaatgta
      961 tcgaccagct tttgatcaag agagacacga ttgtcttctg ttacgtattt ttgagcgata
     1021 cgacgcatga taataggctg ttcgtcgatt tctttcagca tataaaaagg ataggtacct
     1081 ttctcgatat ctgaagcatc tacttgtgct tcgaatgaag aacgttctac gatgtcacct
     1141 gcaaaatttt gaataataac tttttctgat gtgacggtaa ccatctcttc atcttcaatc
     1201 tcaacgaatt ccttcgtatg agcaagcata gccattgcat cgctggcaat cacattgaat
     1261 ccatcgccga gtccgattaa tagtggactt ttatttttag cgacgtaaat aacatcaggt
     1321 gctgtccgat caatcaaagc gaaagcgtaa gaacctttga tgacccgcaa agcttttaaa
     1381 aacgcctctt gcgttgtagt atccttcgcg aaagtttcaa ttagatgagc gataatctca
     1441 gtatcggtct caccaataaa gtggtcattt gctaaataag caattttaag ttcttcaaag
     1501 ttttcgatta cgccgttgtg aacgagcacg aagcgtccac tttgggaagt atgtggatgg
     1561 gcgttttctt cagaaggttg cccgtgagtt gcccatcgtg tatgaccaat acctatattt
     1621 cctgtagtat caatatttat tttgtttttc aaattttgaa ttctgccttg tgctttgacc
     1681 agataatcag ttgtgccatc tgagacaaag ataccagctg aatcataacc acggtattct
     1741 aatttttcta gaccgtcaat caatcctggt gttacatttt ttaatccaat cattccaaca
     1801 attccgcaca taaaaaacat cctactttct ataatgaatt cgtatgaatg cttccagctg
     1861 caggaaaatc cttttgtttt atctttgcag acattttttg tggattttct gtagagtgca
     1921 gtccctaggg ccatccgccg aatctttcga taaaccctct cctcgtcatc tgtttacaca
     1981 gacctggcgc tatcctaaaa ggtataacta tcctcctttt tgttttttat ctggaaacat
     2041 ttaaaatcat aaccttttgc gaatgttctt gtcaacaaaa aaagaataaa aaataaattg
     2101 gtatatatca aattctttgt caaatgtcct ttttatgtca aaaaataatg ggtttttatc
     2161 tatcatcttt ttcaggagtt cggtacaata gaaagtaatc ggatagagga ggagtaaaaa
     2221 tggattcaaa aattgagatc atgacactgg gaatgttaaa aaaacaattg tccaaattcg
     2281 aagcatcagc tggtgtatca gatgatacga aaatattttt agatactggc tgggatagca
     2341 tccaagaaat cgcacctgat gccttggaag ttgctcaagc tcgtgagttt acggtagaag
     2401 atgaattaac aaaagagtcg ttttccgggt atgcgcggga agaaaaagca gagcggttcg
     2461 acacttctga gcagtcagaa actgtgatcg tcataaaaaa cttgtattag aaaggagaaa
     2521 gaatgaataa aagacgttgg gcagcagttc tgattgctgc aggattattt gtggtttcgt
     2581 tagtttcagc tagtttgact aagcctcagg aaaacgatca aattgaggga ttgaacagtt
     2641 ggttatatgg ggatgaagaa ctagctccaa atgtgttgga agagggagac agtacgcaaa
     2701 aaatcgtaaa attatcagta gatggaacga ttgctgcggg agggtcttca ggtttatttg
     2761 cttctgaagg ttacaatcat aaaaatttta tggaacaact gaaggcgatt gaagaagatc
     2821 caacagtcaa aggtatcttt ttagaagtaa attctcctgg tggcggtgtc tacgaaagtg
     2881 cggaaatcgc taagaaattg gatacgattc gtaaagaaca tgatatccca atgtatgtca
     2941 gcatgaaaaa catggcagct agcggtggct actatatttc tgctcaagca gataaaatct
     3001 ttgcaacaga agaaacagtg accggctcaa ttggtgtgat catgtcagga ttgaattatt
     3061 caggacttct agaaaaactg ggtgtggaag atacaacagt taaaagcgga gcgttgaaag
     3121 atatgggttc aggaacgcga ccagagacaa aagaagaaga agcggtgctt caagcatata
     3181 tcgacagcgc atatagtcgt tttgtagcaa tcgtcagcga gggacggaat aaatctgaag
     3241 aagaagtcaa aaaaattgct gatggaagga tttatgatgg tgtccaagca aaagaagtag
     3301 gtttagtcga tgaattggga tttcctgaag atgctttaaa agctatacga aaggaacaac
     3361 agcttgaaga tgcggaacta gtagaatatt cttccacgcc atctgggttt gctagtacat
     3421 ggttaggaag taaactagca gaattccagg gattgaagcc ttctgagact agtcagatct
     3481 tatccgttct tgagaatcta gggacagcgg aatcaccaaa agcaatgtat tattacggag
     3541 gtgagtaagc atgtcagagc tttcaaatga aaataaggaa caagaaaaag ataagcaaca
     3601 gcagcctgct gtaggacctg actccaactt ttcccaaaag gtactgagct cttttgaaag
     3661 agagcctttg acgcccgaag aaatcaaaaa gaaacaacgt aaatggaaaa agtatacgga
     3721 cacaagaaat gaaccgccaa aagcaaatga ttttcctaag tatttctatg caggtttttt
     3781 tatccgaatg tttgccttct tggtcgatct tttgtgtatc ggagctatta cgcgtttaac
     3841 acttggtatt gccaaaaatc taggttggct taatttttca gattcttatc tgagtctcta
     3901 cggattcaca gccttactta tttatctagc gtattttatt ttattgacga aattcaatca
     3961 tggccaaacg attgggaaaa tgatctttgg tattcgagtc atcagctttg atgaagaaga
     4021 acttagctgg tcaaccgttt tagttagaga aggtgcttgc cggttcattc taaaatttcc
     4081 tttgctgatg ttagggtatc ttccagcagt ctttaatcag aaaaagcaac atctgggcga
     4141 ttttttcact aatacaagtg ttgttacgat caatctgatc aaagcgttca accaggaagt
     4201 caacagctga tgagcaataa aaagcagtaa cagttgaaag caccttctaa aagtcagatc
     4261 taaaatctag cttttagagg gtgcttcagt tatttttaat tgtcatgctt tttttgtttt
     4321 gctactattg aacatgagct gaatatgagc aagaggagga cactgcgtga agccgattga
     4381 ttttccagaa aaatatgaga atttaatgcg agtggcacaa caagcactag caaaccagca
     4441 atatcagcag gcaaaagaat tatttcaacg agcttacgag ttgaaagaaa gctttgaggc
     4501 taatagcctg cttgtttttt gtctttacga gttggacgag aaaaaagaag cattgaagca
     4561 agcactcttg catgaaaaac agtatcttca aaatgaggag tttgctgaat tttattttga
     4621 cttgttaatc agtacacaag attttttata tgctagaaaa ttgatagcaa gtacagattt
     4681 ttatgaagct ttcgagcagc gggtcattga aaaaatccaa tttgcagaag aattatccgg
     4741 acaaatggag aggcagaaga tcaaggcact ccacaataaa tcggaggaac ttccttcgtt
     4801 ggaacctgca cgtcagcttg cgtctattga tgagatcgaa cagcttcctt atcatgagtt
     4861 tatccaaaca gcgagtaaac ttattgtatc gccagaggtc catattttag caagggcaaa
     4921 attactggag acacttaggc aattgaacga atgccatcct gttttctatt tgacgattga
     4981 agaaaagtta gtcaaagtca taccaaaaga tctgccaaag ccgcagcagc aggcaagtta
     5041 ccagcaatta tgtgtatttt cagatcgtta cgggaatgaa gatgcgctgc ttagttccgt
     5101 gttaaaagaa gaattcacgt tgcaatctgc aattgtttat cctgtctatg atacatacat
     5161 cgaagacccg aagcgctggt ttcaactgac tgtagaggcg tgtacaggaa aaacgatgta
     5221 tgaaggtaca gaagatgaaa aacaagattt tttaaaaaaa agagaaaaaa ttttgcagca
     5281 aatgatcttc ttccattaga ttttatcagt gaaaaagata aaaatgttat gaaaaacaag
     5341 gattctatcc attcaatatg tttacaattt gaaagggtta gtgtactatt taaaggtatg
     5401 catgaaacgt ttttgatgta taaaatgaat ttctaaactc ggagggaatt aaatgactgc
     5461 aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa tttacgattg cgcaagacga
     5521 aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa aaaagcttga acgtacctgg
     5581 attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt atgtacggtg aagaagcttt
     5641 atatgaagat gcattgaatg ctgtattacc agaagcttat gaagctgctg tcaaagaagc
     5701 tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa agcatggaaa aaggtcaaga
     5761 ctgggtaatc actgctgaag taacagtgaa accagaagta aaattaggac aatataaaga
     5821 tctagttgtt gaaaaacaag accgtgaagt aacagatgct gacgtggatg cacgtatcaa
     5881 acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa gatgctgcag ctgaaaatgg
     5941 cgatacagta gtaatcgact tcgaaggctt tgtagatggc gaagcgtttg atggcggaaa
     6001 aggcgaaaat tactcacttg aattaggttc aggctcattc attccaggtt ttgaagatca
     6061 attagttggt cataaatcag gtgaaagtct agatgtaaat gtaactttcc cagaagatta
     6121 tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa gtgacaatcc atgaagtaaa
     6181 agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa gatgttgatg acgaagtatc
     6241 aacattagac gaattgaaag aaaaatatcg caaagaatta gtagaaacaa aagaaaaagc
     6301 agcggacgat gcaaaagatg aagcagcgat ccgtatggct gttgaaaacg cagaaatcgt
     6361 tgaattgcca cacgtgatgg ttcatgatga agtacatcgt tcaatggacg aattcttgaa
     6421 caacatgcaa cgtcaaggta tctctcctga aatgtactat caattaactg gttctacaga
     6481 agaagacttg cataaacaat tcgaaggtga agcagaaatg cgcacaagaa caaatcttgt
     6541 gatcgaagca attgcagctg ctgaaaatct tcaagcaaca gaagaagaaa tcaatgcaga
     6601 aatccaagaa ttatcagaaa catacaacat gccaatcgaa caaatcaaac gcgtgttgac
     6661 agaagatatg ctggaacatg atgtgacaat gaaaaaagca atcgaattga tcacaggtac
     6721 agcgacagaa gcgaaagaat agaaatcaag taataaatca tttctgaaga agctacttct
     6781 agaaaattgt ttattcgaaa taagggactg tgacacgatt ttgtcacagt tccttatttt
     6841 ttgttaagag taaaaaatgc aaaggtatag gtcagttgat cctttgttaa acactttttc
     6901 tttttactaa actatgttat gatagtaaaa gactgtaaag tctttgatag tttcttgcat
     6961 actttttgca agttaagggg tgaagaccat gtatgacaat ccaagtagca atgagaccgt
     7021 acgctgttct ttttgcggga aaacccaaga agaagtgaaa aagatcgtag ctggtccagg
     7081 ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa atcattgatg aagaatttta
     7141 tgaagaagct gtacgcgaat tcactgaagt ccctaaacca ctagaaattc ttgaagtatt
     7201 taataactac gtgattggac aagatcgcgc aaaacgctcg ttagctgttg ccgtatacaa
     7261 tcactataaa cgtgtcaatc agcaagcaca agaaaacaca gatgatgttg aactgcaaaa
     7321 aagcaatatc tgcttgatcg gacctacagg gtctggtaaa acatttttag cacaaacatt
     7381 agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg acaagtttga cagaagctgg
     7441 ttatgtgggg gaagatgtag aaaacatttt gcttaaattg cttcaatctg ctgattacaa
     7501 tgtggaactt gcagaaaaag ggatcatcta tattgatgag atcgataaaa ttgcgcgtaa
     7561 aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa ggtgtccaac aagccttgct
     7621 gaaaattcta gaaggaactg ttgcaagtgt gccacctcag ggcggacgta aacatccaca
     7681 tcaagaattt attcagattg atacgacgaa catattgttc atcgtaggtg gtgcatttga
     7741 tggcatcgaa acgattgtga agaatcgtat gggcgaaaag acgattggat ttggtacaca
     7801 aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat atcattcctg aagatttact
     7861 aaaattcgga ttgatccctg agtttatcgg acgattacct gttatggcag ctttagagaa
     7921 attgacaaca gatgatctgg tacggatctt gactgagccg aaaaatgcgc tagtcaaaca
     7981 atatcaaaaa cttctttctc tagataatac cgaacttgaa ttcgaacctg aagcgttgag
     8041 agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct cgaggacttc gttccatcat
     8101 tgaagaaatc atgatggatg tgatgtttga tatcccttca aatgaaaaca tcgaaaaagt
     8161 gattatcaca aaagaagcag ctgaattgtc tggtgagcca acagtaatct ataaagattc
     8221 agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa gtcattttgg cttttgcgac
     8281 agccgcttgc tttattcatc accttgttaa ggagagagac catgaaagtc catcaagctg
     8341 aaattatcat aagtgcagtg gcaccaaaac aatatccaga tacacaattg ccagaaatag
     8401 ctctggcagg acgatcaaat gtagggaaat cttcttttat caacacattg attgatcgaa
     8461 agaatttagc ccgtacttcc ggtaagccag gaaagaccca gacattgaat ttttatttga
     8521 tcgagaacgc attacatttt gtcgatgttc cgggatatgg gtatgcaaaa gtatccaaaa
     8581 ctgagcgagc aaaatgggga aagatgatcg agacatattt aacgactaga gaccagctac
     8641 gtgcggtcgt ttctttagta gatttgcgtc ataagccttc tcaagaagat atccagatgt
     8701 atcaattttt gaagtattat gatatccctg taattgttgt tgccactaag gcagataaga
     8761 tccctcgtgg taaatggaac aagcatgaga gcgaaatcaa aaaagcgttg gattttgatc
     8821 gcaatgacac gtttattctc ttttcttccg ttacaaaaca gggaaaagaa gaagcgtggg
     8881 cagcaatcga acaagcaatt gaatcataaa aagaataaaa aagacttacc agtcaaactc
     8941 tttcataaaa gtttggctgg taagtctttt tttgagctta acttactttt gtttcttgtc
     9001 ttttttcttt tcattttgtg gctgtggttt tagaaatcga tcgatctctt cttttggtgc
     9061 aactgttttt tccggatcga cagcaaatga ttcgaaaagt ttatctaaat tatctgtacg
     9121 ttcaaatttc aaccccattg taaacacctc tttgtttctt tttacttagt ctatcatgta
     9181 atcgtccaaa ttggtttcta agacataaag ttttagtttt tctttatcat tggttataat
     9241 taaattataa aaagatttga aagaggtgag aaaatgattg gcgaagataa attattgatg
     9301 actatccagc tcacagactt aaatgaagaa gaaaaaaaac aggaaataag cgattgggct
     9361 attttgacac gtctgttgat ccagccaaca tttatccgct ctttcacaca acaagcggat
     9421 ccgtatgatc ttcgaaagct tcaagaaaaa ataatgctag catcggtaag agacgaaagt
     9481 tggttagttg taggaaatga cgagaacgaa tgtagttttc gcttggaaga taatcaatta
     9541 ttgataaaaa atgtattgtc tatctctgtt ttcaaagaaa agcaattttt gattcgagac
     9601 ttcattcaga aaaaaatgat tgctcatggg gtgttcgcct atatgcgagc ttatagcgag
     9661 tttatctatc acaatacaaa acagatctct caacggctat tgtttgagaa aaaggatgaa
     9721 atcgaacatt tacctaaaat gaaacaacaa aacggggaag tagtagtcga ttgcaaccaa
     9781 tttccaggat acgatctttt ttaccaaggt ttatgtttca cttcgtgttg ggaaatgtat
     9841 tatagtcgct attatcatca aatcatccct aaaccgattt ttctcgacat ccaacaagta
     9901 gaaaaagtga aggaattaga aaacgaagtc atatgtatcc agctttatcg agaccccttt
     9961 aactggcaaa agccaaacaa tcaaatgttt caatcctatt ttagggacca gcttgggttt
    10021 gatcatcttg cttgggacaa tggtgtcggt ttattgaaac cgccattcgt tgaatacatt
    10081 tacactgacc atacgatcca atctgtccag tatcagaatg cacaaatgca acctgttcct
    10141 aaaaagaatg cttcattttt tgtaacaaaa agttatgata tccaacaagg agattataaa
    10201 gaaaggcgtg ttcgcggaac actaaatgct caggcgtatt ttccttgggt tgacgagaat
    10261 cgatcacgca tgatgtgcta taaagttatc gatccgactg ttgctttgga taatggaatc
    10321 gaagcttatt gttattatat tagggaatat ctagaagtag aggtaacaga tgaaaaatat
    10381 caagagtatc ttttatcctt aagaatctat gttccttcag aatccctgtc agaattaccg
    10441 ttaaaggaaa tcaaacatag actctctgat gttaccttca aacgaataaa gaaaaagcgg
    10501 cgaagtatcc aattcgatgt caagaaaggt gagcagcatc tgcgtgtgca atttttagat
    10561 taccgagaat tagaacaatt gattacttta caaaaaatat gaggtgaaga aaatggataa
    10621 acaactctat tatcgaataa tggttccggc atcattatta tccgtctgtc tcttttttgt
    10681 tcctaatttt cctattcgat cagtcatcgg ttttttggtc atgtttggcg
//
LOCUS       DAZAOG010000086        10710 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000086 DAZAOG010000000
VERSION     DAZAOG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.086"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1344
                     /gene="lysA"
                     /locus_tag="ACHDLD_001850"
     CDS             85..1344
                     /gene="lysA"
                     /locus_tag="ACHDLD_001850"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN3363542.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1331..2131
                     /gene="dapF"
                     /locus_tag="ACHDLD_001851"
     CDS             1331..2131
                     /gene="dapF"
                     /locus_tag="ACHDLD_001851"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN3363543.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2134..3015
                     /gene="dapA"
                     /locus_tag="ACHDLD_001852"
     CDS             2134..3015
                     /gene="dapA"
                     /locus_tag="ACHDLD_001852"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN3363544.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3012..3728
                     /gene="dapB"
                     /locus_tag="ACHDLD_001853"
     CDS             3012..3728
                     /gene="dapB"
                     /locus_tag="ACHDLD_001853"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN3363545.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3734..4429
                     /gene="dapD"
                     /locus_tag="ACHDLD_001854"
     CDS             3734..4429
                     /gene="dapD"
                     /locus_tag="ACHDLD_001854"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN3363546.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4445..5446
                     /locus_tag="ACHDLD_001855"
     CDS             4445..5446
                     /locus_tag="ACHDLD_001855"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN3363547.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5443..6804
                     /locus_tag="ACHDLD_001856"
     CDS             5443..6804
                     /locus_tag="ACHDLD_001856"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN3363548.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6764..7915
                     /locus_tag="ACHDLD_001857"
     CDS             6764..7915
                     /locus_tag="ACHDLD_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3363549.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7890..9059
                     /locus_tag="ACHDLD_001858"
     CDS             7890..9059
                     /locus_tag="ACHDLD_001858"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3363550.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9234..10613
                     /locus_tag="ACHDLD_001859"
     CDS             9234..10613
                     /locus_tag="ACHDLD_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3363551.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3556 a         1876 c         2407 g         2871 t
ORIGIN      
        1 ttaatattat tccaataaaa ttcaaatctt tatcaaatga aattccaata aaaacggaaa
       61 aaaagtgaaa agaggaatga aaaaatgaca gaattgataa ttggcggagt cacagcaagc
      121 acattggcag aaaagtataa gacacctctg tatgtgtatg atgaagaaaa gatgagacaa
      181 acgatgctgt cattcaagcg aggctttgta tcgaaacgtt ttgatacaaa agtgctttac
      241 gcctctaaag cattccaaac gattgagatg ctgcatttag ttcgtgaata tggattcggg
      301 ctagatgttg tcagtggtgg ggaaatctat accgctttgc aagctgattt tcctagagat
      361 actatctact tccatggtaa caacaagacg ccagaggaac tgatctatgc acttgaaaat
      421 gatttgttgc atattgttgc cgataatttg atggaagttg aattattagc agaattaagc
      481 caacgctatt taaaaaaaat ctgtatcatg cttcggttga atgtcggagt agaagcacat
      541 actcatgaat atatcgtcac tgcgcatatt gattcaaaat tcggcatgag ctatgaaagt
      601 aaagagtgcc aacaagccct tcagttgata aaagaaagca gctatctaga gttagaagga
      661 tttcatgctc atatcgggtc acaaattttt gatgttactg cttggttagc tgaaatcgac
      721 aaattgacga attacttaac cgattttgac caacctcttt ccttgaatct tggcggaggg
      781 tttgggattc gttatacaca aaatgatcag ccgatcccag tcgaagaggc actaaaaaaa
      841 ctgattgaat atacggaaga agctttggaa aaacgaaaac tgaccattcg tcaattactg
      901 atcgaaccgg gtagaagtat tgttggagaa gctggtacaa cactatacac gattggtttc
      961 atcaaaaaga caccacataa aaaatattac tttgtagatg gcgggatgac agataatatc
     1021 cgtccagctc tgtatcaggc agaatacgat tgtgatttag cagcaaaact gtcggaagaa
     1081 aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat caggcgatgt ggtaattaaa
     1141 gaagcttatt tgccgaaagc agaatcgggt gatcttttag ctgtttattc cacgggagct
     1201 tatggatact cgatgagcag caactacaat cgagcgacac gaccagcagt tgtttttgtc
     1261 aataatggcc aatcaagact gattgttcgc agacaagcat tttctgatct actaaggggg
     1321 gaagtttcct atgatccttc ataaatataa tggctgcgga aatgatttta tcttattaga
     1381 ttacgcagaa gacacagatt atagtcaatt agccgcttct ttatgcacaa aagaaaaata
     1441 tgatacagac ggattgatcg ccgtaaaaat cgatccgttg gaaatgatct attacaacaa
     1501 agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc tgttttgctc gatacgtaaa
     1561 tgatcagaac atgatgaatg aacaggaatt caacgtagag acattagcag gcacaagaaa
     1621 aattacggtg ttggatccct caccttttta ttgcagcgta gatatgggag tgccagatga
     1681 ttctccggaa aaaatcaaag ctaatcaaac ttatccaata aaaaaccagg aaatagagat
     1741 caatggtgaa aaagtaacga tcactagttt atttttagga acgattcata cagttgtttt
     1801 tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt gaactgatct gtcatcatcc
     1861 tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta gtcaatgaac aagaattgat
     1921 cgtccgtacc tatgaaagag gagtaggttg gacacttgct tgcggtacag gttgctgtgc
     1981 ctcttatgtg gttgctaagg aaactgggaa aatttcagca gatcaggtag ttgttcactt
     2041 ggaacaagga gacttgatga tctctggtga ggatgcgata caaatggctg gcccagcagt
     2101 tcatgaatgg acaagaatat tggaggaatg aagatgataa aaggttcgat cgtggcgctg
     2161 atcacgccga tgaatgaaga gggatcagta gattatgctg gtttggaaaa attgatccaa
     2221 ttccatttag atgaacaaac agatggatta ttagttttag gtacaacagg agaatcttct
     2281 acgctcacac aatctgagga ggagcagatc cttcaactga cagtaaagaa agtggctggc
     2341 cgtgtgccgg tgattgcagg agctggaacg aataatacga aagaaacgat tgaaaaagcg
     2401 aagcattttg cttctttggg ggcagacgca cttttggtca tcacccctta ctacaacaaa
     2461 acaagtgatg caggactagc tgctcatttt acagcaattg cagaagcctc accgattccg
     2521 cttattttgt ataatgttcc ttctagaaca ggcatgtcga ttccaatcca tgtattagta
     2581 aatttagcag aacatcctaa tatcattggt ttgaaagaag cctcaggaga tatggcttat
     2641 gtgatggatg ccgctcggtt gattggagaa gaattcttcc tttacagtgg aaatgatgat
     2701 ttgatcctac cagtaatgag tgtaggagga agcggtgtaa taagtgtatg ggcgaatatc
     2761 caacctaaaa tagtccatga attagtaaaa gatacgcagg acggaagatg gcagcaagca
     2821 aaagaaaagc aattaaatgc acttgaactg atccatgcct tgttcagtga gaccaatcct
     2881 attcctgtca aagcagccat gtcattatta gacttacctt ccggacctct acgcctgcct
     2941 ttagtttctc tttcaaaaga gaaaaagaaa caattagctc agcttttgtt aaaagaaagg
     3001 acggttgaaa aatgaaaatc ggactgattg gcagtggaca gatgggaaat agagtcgaag
     3061 aagttgcaca agaaagagga gatacggtcc ttcttttacg gacggcacct aaagaagcaa
     3121 ctacgcttgc ttttacggaa ctgccagaac tgcttattga tttttctcat ccagataatt
     3181 tagagaaaat cctttcgtat agcttatcat atcaacttcc gttagtcatt ggcacgacag
     3241 gttatacaca atcacagttc caagaaataa aagaacatgc caaacatgta cctgtgatgt
     3301 atagtgctaa tttttcgttc ggcatcatgg tgatgaatca actaatcaag caagcagctg
     3361 caatgttgca aggctggcag atcgaactga ttgaaaaaca tcatagtcgt aaaaaagatg
     3421 caccatctgg tactgcaaat atgctgcttc aaacaatcca agaagtgcaa aagctgcagc
     3481 ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca aattggtgta cacagtattc
     3541 gtgcaggttc tttacctggt gaacatgatg tattgtttgc tgctactgat gaaatattta
     3601 cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc aggcgcagta gaagcagcgg
     3661 actggttgaa agatcagtca ccaggttatt acaaattaga agatttatta atggacaaag
     3721 gggtgtaaaa aacgtggaag ctcaagaaat catccgctac attcaaacgg catctaaaaa
     3781 gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc gaatttaaag aaagtaaagt
     3841 ttttggtgaa ggaagcagct tcgttgtttt tggtgactat gagacgattg ctccggtttt
     3901 agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa actgctgcaa gatacagcgc
     3961 agttcctttg ctagatatta aaaaaattaa tgctcgtatc gaaccaggtg ctattatcag
     4021 agatcaagtg tcaatcggca acaatgccgt gatcatgatg ggagcgatta tcaatattgg
     4081 tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct gtcttaggtg gacgagcgac
     4141 agtgggcaaa aactgccata ttggtgcagg agctgtctta gcaggtgtga tcgaaccagc
     4201 atcggccaaa ccggttatcg tagaagacgg cgtactggta ggtgcgaatg cagtgattgt
     4261 tgaaggtgtc catatcggaa aagatgcagt cgtggcagca ggagcagtgg ttttagaaga
     4321 tgtggcagca gaaactgttg tcggtggaat acctgcacgt gtattaaaaa taagtgatga
     4381 tcagacaaaa gaaaatacag cattgattgc tgcgttacgc gaattataga ggagatggag
     4441 aaaaatgcgc gaaatagata cctgtattgt gggggcaacc ggattagtcg gccaaacgat
     4501 gctcaaagtg ttagaagaat atgattttcc agtaggacga ttgaaattat tagcttcaaa
     4561 acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa cacactgtac aggaattgac
     4621 agaaacttca tttgaaggat atgatttagc gctattttct gcaggtgctt ctgtatccaa
     4681 agagtttgcg ccaatcgcac gaagtcaggg actatttgtc atagataatt cctcagcatg
     4741 gcgggaagat tcggatattc cattaatcgt tccagaggtc aatctggaag actatacatt
     4801 gaatcggctg attgcaaatc caaattgttc aaccattcaa gcggtacttc cgcttaaagc
     4861 tttacaagaa gcctttggta tccagcgagt caattattcg acctatcaag cagtttctgg
     4921 ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa aatgacgagg aaccagtttt
     4981 gtatccttat aatattagcc aaacggtgat tccggaaatc gatcagcctt tggaaaatgg
     5041 gtatacaaaa gaagaacaga aaatgataaa tgagacccgt aagattctgc atcagcctgc
     5101 tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa aatggacatg gagtctctgt
     5161 ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt agagagtgtc tcagccagtt
     5221 tccagggatt caattagaag atgatctagc agcacataaa tatccgacga gcattcttgc
     5281 tagaggaaca gatatggttt atgttgggag gatcagaaaa gacacttctt tagagaacgg
     5341 attgttgttc tatacgactg ccgataacat acgcaaagga gccgctgcga atgccgtaca
     5401 gattgctgca gctttaatgc agaaaatagc ggaggtggca agatgagtaa tacgctgaaa
     5461 gtagcgaaat tcggcggaac atcgatgtct catgaagagc aattcaaaaa agtccgtaca
     5521 attgttcttt cagatcccaa tcgccaagtg atcgttgttt ctgctttagg caaacgtacg
     5581 tcagatgatg acaaagtaac agacttactt tatttgattt atgcttacct tcagcatcat
     5641 gttgattgga cacccctttg gaaaaggatt tgtgatcgtt tcattcaagt aagagatgtt
     5701 cttgccctgc agttacctat tgaagatgaa ctagcacgca tacagaaaca actggaagac
     5761 gaaaagataa ctgaagatta tttagtaagt agaggtgaat atctgactgc tcaactgatg
     5821 gctgagtatc ttggttacca gtttattgac gcaaaagacc tgattttctt tgactatgat
     5881 gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa aagcatttaa aagatatccg
     5941 aaaatggttg ttccagggtt ttacggggcg aaccctgcag gaaaagaaaa attattgggc
     6001 cgtgggggaa gcgacattac cggcgcgata ctggcgaaga tgttgcaagc ggaaagttat
     6061 gagaattgga cggatgtttc aggaatcatg atggctgatc cgcgaatcat cgatcatcct
     6121 aaaatcatcg aggaaatctc ttttagagaa ctgagagaga tggcttatat gggggctaac
     6181 gttcttcatg aagcagctgt tttcccagtt caagaggctg atatccccat ccaaataaaa
     6241 aataccaatg atcctttcgc tcaagggaca aaaatatcga atcacgaaat agaaaaggaa
     6301 aatggactga caggaatagc tggacgcaag gattttcttt ccatcacttt attcaaacgt
     6361 catatgtccg atgaaatcgg gtttatctgg aaagcaatga gcatttttgc taaacatgag
     6421 atatcaatcg aacatattcc ttcagggatc gacaatatcg gagtgatcgt ctcagcagaa
     6481 gcgattgccg ataagttatt tctcatcacg aaagaattaa aagaaacact tggcgtggaa
     6541 gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg ttgggggtcc gcataaagaa
     6601 ttgattggat tatctgggaa agtgttatcg atattgaaca aattagaaat tcgcacatcg
     6661 atcttatcac aaggagcaca ggagttgaat ctgatcatcg gtgtaccgaa taatcaatat
     6721 gaaacagtag tgaagggtat ctatgaaggg atggtgaata cggatgctga aagaatatcg
     6781 aatggcactc catcaaattc ctgaaatcgg gtttcatgaa ttcaagacaa aaaaatattt
     6841 atatgaacaa gtaaaaaaca gcggggccgt tatccatgaa atcggcgaaa caggactact
     6901 cctttatttt gataatcagc aagaagaaac gattgctttt cgaacagata tcgatgcact
     6961 gccagtcaaa gaagaaacga atctctcttt tgcttctaag catgaaggtt atatgcatgc
     7021 atgtggtcat gacggacata tggcgatgct tttagggttg actgacttta taactaaaca
     7081 cattgaggat cttatttata atgtagtact gatctatcag ccatcagaag aaatttccgg
     7141 tggcgcacaa agcgttattc agtcaggatt gttggataaa tatgaagtac aagcaatatt
     7201 tggctttcat ctatggcctg gactagctaa gggagaagtt ttctctcgac caggaccttt
     7261 gatggcacaa agcagtgaaa cagacattat cattaaaggc cagtctgcac atattgcttc
     7321 tagtgaaaaa ggaatcgata gtctggaggc agctgtacga tttttgaaga atgtttatga
     7381 ttttgaccgg caattaccgg cagagtggaa tcatctaatg aaatttggcc aaatccaagg
     7441 tggaacgatc cgcaatgttt tagctggaga agttattgta tccggaagta tccgttctta
     7501 cagttcagat atgcaaaatt atttgaagag gggattagac catttagcag aacagtttca
     7561 ctctacagct actatttctt ttcgctataa tgacggctat ccagctgtaa tcaaccatag
     7621 aggcttatat gcagcactgt tagattcaaa caaactacat gaattagatg ctccagttct
     7681 tcaagccgaa gattttggca tatatacgca acaatatcca tgtgtatttt tctttttagg
     7741 tgtcggtgat acccctgctt tacatgatcc gacgttcgat tttgatatgg atgttttaga
     7801 aaatggtgta gaatggtaca aaacgattct ttttacagaa aatcttcttg taaaaatcga
     7861 tagcagttca aaagaggagc gagtaaataa tgatgaattt atccaatcga tttagtccta
     7921 ggctttctag aatagaagta tcaaaaatac gacaattcga tcagcaaata tcgtcgatac
     7981 cagatgtgat caaactcacc ctgggagaac cggattttcc aactcctgaa catgtcaagc
     8041 aagcaggaat agcagcgatt gaagaagact tttctcacta taccggaatg agaggattag
     8101 aggaactgcg ggaagcggct tgtatatttc agcaacagcg ttatggatta acttatgatc
     8161 cgcaaacgga agttttgacg acagttggtg caacggaagc gattgcctct gcgttgttat
     8221 ctgttttgga agaaggagac aaggttctta ttccagcgcc tgcttattca ggatatcagc
     8281 cattagtgga actagctggt gctgaattga tcccgataga tacttctgac acagggtttg
     8341 tctgtcagcc ggaacaattt gaacgagcat ttgaacaata tggatcagct gtcaaagcag
     8401 tcattttaaa ctatcctaac aatcctactg ggacgacatt gtctgcaaaa caattgaaag
     8461 caatcgctga ggttttaaaa aaatatccgg tatttgttat aagtgatgaa gtatatgctg
     8521 aattgactta tagtggaacg catatgtcca tagctactta tcttccagaa caaacaatcg
     8581 tgataagcgg gttatccaag tcacatgcga tgactggttg gcgagtgggc tttatttttg
     8641 ctcaaaaagc ccttattgac gaactaatta aagtccatca atatctggtg actgccgcta
     8701 caacgatgtc gcaaaaagct gcggctgaag cgctaataaa tggggtgaaa gatagcgaga
     8761 atatgaaaga gcaatatcaa acacgaagag actatctggt ccagcaattg gcgcctttag
     8821 gatttgaagt gacccagcca aacggtgcat tttacttgtt ttgcaaactc ccagctgcca
     8881 ttcaaactga ctcttggcag ttttgtgtag aattagcgga acaggcaaaa gttgcgtgta
     8941 tcccaggaat tgcatttggc ccagaaggag aaggttatat ccggatttct tatgctagca
     9001 gcatggaaaa attacatgag gcctgcaaac ggatcaaaga ctttttaaca gaacagtgat
     9061 tcatagattt tatcaaaaaa acaatcgtta attttgcggt atattctgca aggagacgat
     9121 tgttttttaa atttagttca aatgaacgcg tttcttttga ttcataacta atgtaatgtt
     9181 aagtttaaat tgtttagaat gattttaaaa gaatattcaa ggaggtttca tcaatggaaa
     9241 aaatcaaagt tgtcattgta ggtgcatctc atggagggca tcaatcgatc ttggaactat
     9301 tgacacgata cggtgaaaat gtagatatca cgctatttga agcaggagac tatatttcct
     9361 tcatgtcatg tggtatggag ctgtatttag aagacaaggt aacagatgta aatgatgtac
     9421 gtaatttcag accagaaaac tttccgcaat ccaatgttca tattttaaat aatcacgaag
     9481 taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca gataacgaaa
     9541 caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca aacaatctgc
     9601 cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat tgggcaacca
     9661 aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta ggtgcgggtt
     9721 acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc gtattgcttg
     9781 atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat attttagaac
     9841 aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa gcttttactg
     9901 gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca gatctagtca
     9961 ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc gacttagatg
    10021 aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat gtgtatgcag
    10081 taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg atcgctttag
    10141 cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa gaagtaccaa
    10201 acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac tatcattttg
    10261 cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta cgagtgtctt
    10321 attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat ccaaaagtgt
    10381 ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca gtattatcta
    10441 cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac aaactgactt
    10501 tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga caatggagca
    10561 tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa taagaaaacg
    10621 aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag aacagccaag
    10681 taaaaacagc ttcttatgtt tttaacttgg
//
LOCUS       DAZAOG010000087        10650 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000087 DAZAOG010000000
VERSION     DAZAOG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.087"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACHDLD_001860"
     CDS             34..657
                     /locus_tag="ACHDLD_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363552.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACHDLD_001861"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACHDLD_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3363553.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACHDLD_001862"
     CDS             2153..3034
                     /locus_tag="ACHDLD_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363554.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACHDLD_001863"
     CDS             3037..3807
                     /locus_tag="ACHDLD_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363555.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACHDLD_001864"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACHDLD_001864"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN3363556.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACHDLD_001865"
     CDS             5118..6281
                     /locus_tag="ACHDLD_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3363557.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACHDLD_001866"
     CDS             6398..6808
                     /locus_tag="ACHDLD_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3363558.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACHDLD_001867"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACHDLD_001867"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3363559.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACHDLD_001868"
     CDS             9381..9665
                     /locus_tag="ACHDLD_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363560.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACHDLD_001869"
     CDS             9825..10061
                     /locus_tag="ACHDLD_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3363561.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACHDLD_001870"
     CDS             10058..10456
                     /locus_tag="ACHDLD_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN3363562.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2902 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat
//
LOCUS       DAZAOG010000088        10506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000088 DAZAOG010000000
VERSION     DAZAOG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.088"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..1616
                     /locus_tag="ACHDLD_001871"
     CDS             192..1616
                     /locus_tag="ACHDLD_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN3363563.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(1658..1915)
                     /locus_tag="ACHDLD_001872"
     CDS             complement(1658..1915)
                     /locus_tag="ACHDLD_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363564.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            2077..3186
                     /gene="pdhA"
                     /locus_tag="ACHDLD_001873"
     CDS             2077..3186
                     /gene="pdhA"
                     /locus_tag="ACHDLD_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN3363565.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3189..4166
                     /locus_tag="ACHDLD_001874"
     CDS             3189..4166
                     /locus_tag="ACHDLD_001874"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN3363566.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4191..5834
                     /locus_tag="ACHDLD_001875"
     CDS             4191..5834
                     /locus_tag="ACHDLD_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN3363567.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            5842..7248
                     /gene="lpdA"
                     /locus_tag="ACHDLD_001876"
     CDS             5842..7248
                     /gene="lpdA"
                     /locus_tag="ACHDLD_001876"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN3363568.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            7410..7685
                     /locus_tag="ACHDLD_001877"
     CDS             7410..7685
                     /locus_tag="ACHDLD_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN3363569.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7691..8458
                     /locus_tag="ACHDLD_001878"
     CDS             7691..8458
                     /locus_tag="ACHDLD_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN3363570.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8626..10455
                     /gene="typA"
                     /locus_tag="ACHDLD_001879"
     CDS             8626..10455
                     /gene="typA"
                     /locus_tag="ACHDLD_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN3363571.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         3579 a         1850 c         2316 g         2761 t
ORIGIN      
        1 tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat
       61 cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa
      121 aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa
      181 aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat cgtcaaatta
      241 tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt caacttgaat
      301 cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc catgcattgg
      361 aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg tttgttaatc
      421 agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg atctctcctc
      481 gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag atccaaaaaa
      541 catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg aattttgagt
      601 ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat cttttaggct
      661 atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa ttggcatttt
      721 cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg attttacaaa
      781 cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt taccgattac
      841 ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa gaagggatcg
      901 acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg cttatcaacc
      961 cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag
     1021 aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg gatctttcat
     1081 ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat gtgttatacg
     1141 tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg ataggacctg
     1201 agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg atgaatatcc
     1261 ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa atgcaagatg
     1321 taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat tcacaatcgt
     1381 ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg gatgggaaaa
     1441 aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt gcaccaagtc
     1501 tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg gatgaaaacc
     1561 aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca gaataatttc
     1621 acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc tgtttctttt
     1681 ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt gtacgtccgt
     1741 tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca acgtttttaa
     1801 atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat gaaaaataaa
     1861 acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt ggcataattt
     1921 cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca tgataattta
     1981 ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg cagaatgaag
     2041 agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa agcaccgatc
     2101 gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca agcacttgat
     2161 caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga tgaactcgtt
     2221 gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac agcgctgaac
     2281 cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag tcagctggct
     2341 agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga tgtacctcaa
     2401 ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg acatgctgca
     2461 ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat cggtgcacaa
     2521 tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa aaatgtcgtc
     2581 ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc aatcaatttc
     2641 gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta tgcgatctct
     2701 acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt cgcagctggg
     2761 attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt atctaaaatg
     2821 gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact gacttatcgt
     2881 tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa agacacagat
     2941 gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac ggaaaaagga
     3001 ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga aatcaaagca
     3061 gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt gaaaaacatg
     3121 ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc gaaggagtcg
     3181 aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta gcacttgaac
     3241 tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac ggaggagttt
     3301 tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc gatactcctt
     3361 tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga tttcgtccag
     3421 ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta gtagctcaaa
     3481 tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca attcgttcgc
     3541 catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa ggtttgatcg
     3601 ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca aaaggactat
     3661 tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg aaactatatc
     3721 gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat aaagcagcag
     3781 ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg cgtgaagcaa
     3841 tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt gatcttcgta
     3901 cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact ggtcgcgtag
     3961 ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt tctgagatct
     4021 cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca cctgatacag
     4081 tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat atcgaagaca
     4141 aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc atggcttatc
     4201 aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg
     4261 tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat
     4321 cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt gttccagaag
     4381 gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga cacgaagaca
     4441 atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg gctgctgaac
     4501 ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa tttaagttac
     4561 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
     4621 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
     4681 ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc actgtagcaa
     4741 atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca ccgtcaacta
     4801 gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg
     4861 ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt caatttgccc
     4921 gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa
     4981 aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag
     5041 cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac
     5101 cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca atgacgccaa
     5161 cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca cacgtgacat
     5221 tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt aaagaagttg
     5281 ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct ttaacagcga
     5341 ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt
     5401 ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta tatgtaccaa
     5461 atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc aatgagaaag
     5521 caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga acaattacga
     5581 tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac tatccagaag
     5641 tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa
     5701 tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc gtagatggag
     5761 caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca gagctattaa
     5821 tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt agatacagtt
     5881 gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga aatgggacaa
     5941 aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt cggctgtatt
     6001 ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga ttctgaaatg
     6061 tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga atggaaagaa
     6121 aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa acataaggtt
     6181 gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt cattcatcca
     6241 gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc ccgtccaatc
     6301 gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg attagcattg
     6361 aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc
     6421 ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc acaaatttta
     6481 ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa aaaaggcgta
     6541 acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg
     6601 aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat ggtcactgta
     6661 ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga agtaggagaa
     6721 cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat ctatgccatc
     6781 ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc aaaaatcgct
     6841 gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc ggcagtagcc
     6901 ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa agatgctggt
     6961 atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt atcgcttggt
     7021 aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct tattggcgcg
     7081 caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc aatcgaatca
     7141 ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct tggcgaaatc
     7201 gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt tgaataatac
     7261 aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc aataatatta
     7321 ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg
     7381 aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag
     7441 attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga
     7501 atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg
     7561 tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt
     7621 ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg
     7681 ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga
     7741 gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct
     7801 agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga
     7861 tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg
     7921 aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa
     7981 tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga
     8041 cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga
     8101 gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta
     8161 tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg
     8221 tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta
     8281 tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg
     8341 tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt
     8401 agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa
     8461 aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa
     8521 aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa
     8581 aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac
     8641 gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat
     8701 gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg
     8761 gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta
     8821 gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc
     8881 gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa
     8941 ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca
     9001 atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa
     9061 gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt
     9121 tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca
     9181 accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc
     9241 gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc
     9301 ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa
     9361 ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa
     9421 cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat
     9481 atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac
     9541 attgacgaac caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga
     9601 gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca
     9661 gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt
     9721 ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa
     9781 gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa
     9841 cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta
     9901 cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc
     9961 ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt
    10021 tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc
    10081 ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc
    10141 atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga
    10201 atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa
    10261 caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa
    10321 caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca
    10381 ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct
    10441 aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag
    10501 ctggtt
//
LOCUS       DAZAOG010000089        10388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000089 DAZAOG010000000
VERSION     DAZAOG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.089"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1586
                     /gene="arcD"
                     /locus_tag="ACHDLD_001880"
     CDS             165..1586
                     /gene="arcD"
                     /locus_tag="ACHDLD_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN3363572.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1600..2718
                     /locus_tag="ACHDLD_001881"
     CDS             1600..2718
                     /locus_tag="ACHDLD_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN3363573.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2778..4070)
                     /locus_tag="ACHDLD_001882"
     CDS             complement(2778..4070)
                     /locus_tag="ACHDLD_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN3363574.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4220..4780
                     /locus_tag="ACHDLD_001883"
     CDS             4220..4780
                     /locus_tag="ACHDLD_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3363575.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4909..5286
                     /locus_tag="ACHDLD_001884"
     CDS             4909..5286
                     /locus_tag="ACHDLD_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN3363576.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5219..7291
                     /locus_tag="ACHDLD_001885"
     CDS             5219..7291
                     /locus_tag="ACHDLD_001885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3363577.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(7502..8449)
                     /locus_tag="ACHDLD_001886"
     CDS             complement(7502..8449)
                     /locus_tag="ACHDLD_001886"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3363578.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            8695..9159
                     /locus_tag="ACHDLD_001887"
     CDS             8695..9159
                     /locus_tag="ACHDLD_001887"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN3363579.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(9221..10264)
                     /locus_tag="ACHDLD_001888"
     CDS             complement(9221..10264)
                     /locus_tag="ACHDLD_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN3363580.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         3463 a         1939 c         2155 g         2831 t
ORIGIN      
        1 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
       61 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
      121 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
      181 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
      241 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
      301 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
      361 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
      421 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
      481 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
      541 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
      601 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
      661 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
      721 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
      781 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
      841 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
      901 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
      961 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
     1021 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
     1081 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
     1141 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
     1201 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
     1261 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
     1321 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
     1381 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
     1441 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
     1501 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
     1561 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
     1621 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
     1681 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
     1741 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
     1801 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
     1861 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
     1921 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
     1981 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
     2041 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
     2101 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
     2161 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
     2221 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
     2281 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
     2341 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
     2401 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
     2461 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
     2521 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
     2581 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
     2641 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
     2701 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
     2761 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
     2821 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
     2881 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat
     2941 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
     3001 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
     3061 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
     3121 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
     3181 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
     3241 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
     3301 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
     3361 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
     3421 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
     3481 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
     3541 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
     3601 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
     3661 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
     3721 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
     3781 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
     3841 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
     3901 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
     3961 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
     4021 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
     4081 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
     4141 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
     4201 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
     4261 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
     4321 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
     4381 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
     4441 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
     4501 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
     4561 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
     4621 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
     4681 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
     4741 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
     4801 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
     4861 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
     4921 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
     4981 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
     5041 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
     5101 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
     5161 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
     5221 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
     5281 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
     5341 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
     5401 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
     5461 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
     5521 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
     5581 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
     5641 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
     5701 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
     5761 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
     5821 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
     5881 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
     5941 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
     6001 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
     6061 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
     6121 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
     6181 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
     6241 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
     6301 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
     6361 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
     6421 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
     6481 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
     6541 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
     6601 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
     6661 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
     6721 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
     6781 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
     6841 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
     6901 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
     6961 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
     7021 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
     7081 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
     7141 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
     7201 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
     7261 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
     7321 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
     7381 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
     7441 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
     7501 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
     7561 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
     7621 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
     7681 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
     7741 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
     7801 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
     7861 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
     7921 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
     7981 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
     8041 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
     8101 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
     8161 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
     8221 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
     8281 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
     8341 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
     8401 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc
     8461 tttcaatcgt atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc
     8521 tttaaaattt gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt
     8581 ttaccatgtt tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag
     8641 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
     8701 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
     8761 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
     8821 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
     8881 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
     8941 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
     9001 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
     9061 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
     9121 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
     9181 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
     9241 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
     9301 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
     9361 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
     9421 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt
     9481 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
     9541 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
     9601 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
     9661 attggagtgt accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
     9721 atccaagaaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
     9781 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag
     9841 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
     9901 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
     9961 ttccttttac accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
    10021 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
    10081 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact
    10141 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
    10201 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
    10261 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
    10321 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt
    10381 ttcgaata
//
LOCUS       DAZAOG010000090        10255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000090 DAZAOG010000000
VERSION     DAZAOG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.090"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..277)
                     /locus_tag="ACHDLD_001889"
     CDS             complement(<1..277)
                     /locus_tag="ACHDLD_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN3363581.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
     gene            605..1084
                     /locus_tag="ACHDLD_001890"
     CDS             605..1084
                     /locus_tag="ACHDLD_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN3363582.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1117..3345
                     /locus_tag="ACHDLD_001891"
     CDS             1117..3345
                     /locus_tag="ACHDLD_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3363583.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3474..5117
                     /locus_tag="ACHDLD_001892"
     CDS             3474..5117
                     /locus_tag="ACHDLD_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN3363584.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5190..5735)
                     /locus_tag="ACHDLD_001893"
     CDS             complement(5190..5735)
                     /locus_tag="ACHDLD_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN3363585.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5758..6243)
                     /locus_tag="ACHDLD_001894"
     CDS             complement(5758..6243)
                     /locus_tag="ACHDLD_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3363586.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6233..7045)
                     /locus_tag="ACHDLD_001895"
     CDS             complement(6233..7045)
                     /locus_tag="ACHDLD_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN3363587.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7184..8716)
                     /locus_tag="ACHDLD_001896"
     CDS             complement(7184..8716)
                     /locus_tag="ACHDLD_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN3363588.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8825..9856)
                     /locus_tag="ACHDLD_001897"
     CDS             complement(8825..9856)
                     /locus_tag="ACHDLD_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363589.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3405 a         1904 c         1967 g         2979 t
ORIGIN      
        1 acttacattt aaatcgttgt ttcttcagct ccagataagt gcgatactca gacacatcat
       61 tcagtaacaa ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa
      121 gacaatggga acactcttct ggcttgtaca gaagagttcc tttgtaaatc aagcaaattt
      181 gccctttgta tttttcttta gttaaacaat tttcctcgaa atagatattt aggtctagaa
      241 tatttagggt atctttgata agctgagtat gagacatctt gaatcctcct atgaattggt
      301 agccaaatta attctaaagg atttcagggt gtcttttcta tattttttta tacaaaaaag
      361 atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca acatcatctt
      421 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
      481 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
      541 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
      601 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
      661 taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac tgggccttgc
      721 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
      781 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
      841 agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
      901 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
      961 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
     1021 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
     1081 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
     1141 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
     1201 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
     1261 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
     1321 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
     1381 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
     1441 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
     1501 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
     1561 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
     1621 cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat ccgagatgcc
     1681 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
     1741 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
     1801 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
     1861 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
     1921 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
     1981 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
     2041 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
     2101 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
     2161 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa agaaagaacc
     2221 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
     2281 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
     2341 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
     2401 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
     2461 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
     2521 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
     2581 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
     2641 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
     2701 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
     2761 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
     2821 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
     2881 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
     2941 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
     3001 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
     3061 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
     3121 ggtcagccaa aagcattgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
     3181 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
     3241 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
     3301 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
     3361 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
     3421 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
     3481 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
     3541 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
     3601 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
     3661 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
     3721 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
     3781 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
     3841 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
     3901 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
     3961 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
     4021 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
     4081 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
     4141 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
     4201 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
     4261 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
     4321 aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc gcttggcaaa
     4381 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
     4441 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
     4501 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
     4561 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
     4621 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
     4681 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
     4741 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
     4801 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
     4861 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
     4921 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
     4981 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
     5041 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
     5101 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
     5161 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
     5221 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
     5281 acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat atcgataccg
     5341 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
     5401 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
     5461 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
     5521 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
     5581 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
     5641 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
     5701 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
     5761 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
     5821 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
     5881 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
     5941 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
     6001 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
     6061 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
     6121 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
     6181 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
     6241 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
     6301 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
     6361 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
     6421 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
     6481 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
     6541 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
     6601 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
     6661 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
     6721 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
     6781 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
     6841 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
     6901 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
     6961 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
     7021 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
     7081 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
     7141 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
     7201 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
     7261 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
     7321 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
     7381 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
     7441 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
     7501 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
     7561 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
     7621 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
     7681 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
     7741 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
     7801 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
     7861 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
     7921 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
     7981 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
     8041 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
     8101 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
     8161 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
     8221 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
     8281 aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt
     8341 ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg
     8401 ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa
     8461 cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt
     8521 tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt
     8581 aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg
     8641 tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg
     8701 ttatggtctc catcattcac actccattat cttttgttaa gtaatataca aattcaagat
     8761 aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt
     8821 tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca
     8881 tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat
     8941 ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata
     9001 gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag
     9061 gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg
     9121 ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat
     9181 tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac
     9241 gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg
     9301 caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt
     9361 caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag
     9421 ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc
     9481 ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt
     9541 aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag
     9601 tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa tggataatca
     9661 gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat
     9721 cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact
     9781 gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc
     9841 tagttttgct gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca
     9901 tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt
     9961 actagctaaa agatatgaag taagaagata catttttagt tattttatta gtgcagtcat
    10021 tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat
    10081 gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag
    10141 aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat
    10201 attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt acttt
//
LOCUS       DAZAOG010000091        10180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000091 DAZAOG010000000
VERSION     DAZAOG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.091"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..488
                     /locus_tag="ACHDLD_001898"
     CDS             15..488
                     /locus_tag="ACHDLD_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFN3363590.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            497..724
                     /locus_tag="ACHDLD_001899"
     CDS             497..724
                     /locus_tag="ACHDLD_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363591.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1360..1731
                     /locus_tag="ACHDLD_001900"
     CDS             1360..1731
                     /locus_tag="ACHDLD_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363592.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1987..2229)
                     /locus_tag="ACHDLD_001901"
     CDS             complement(1987..2229)
                     /locus_tag="ACHDLD_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3363593.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2261..3163)
                     /locus_tag="ACHDLD_001902"
     CDS             complement(2261..3163)
                     /locus_tag="ACHDLD_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3363594.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3176..3364)
                     /locus_tag="ACHDLD_001903"
     CDS             complement(3176..3364)
                     /locus_tag="ACHDLD_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN3363595.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3378..3941)
                     /gene="amaP"
                     /locus_tag="ACHDLD_001904"
     CDS             complement(3378..3941)
                     /gene="amaP"
                     /locus_tag="ACHDLD_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN3363596.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3979..4872)
                     /locus_tag="ACHDLD_001905"
     CDS             complement(3979..4872)
                     /locus_tag="ACHDLD_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3363597.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4950..5873)
                     /locus_tag="ACHDLD_001906"
     CDS             complement(4950..5873)
                     /locus_tag="ACHDLD_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3363598.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5922..6272)
                     /locus_tag="ACHDLD_001907"
     CDS             complement(5922..6272)
                     /locus_tag="ACHDLD_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3363599.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6305..7207)
                     /locus_tag="ACHDLD_001908"
     CDS             complement(6305..7207)
                     /locus_tag="ACHDLD_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN3363600.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7200..8057)
                     /locus_tag="ACHDLD_001909"
     CDS             complement(7200..8057)
                     /locus_tag="ACHDLD_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN3363601.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8391..9200
                     /locus_tag="ACHDLD_001910"
     CDS             8391..9200
                     /locus_tag="ACHDLD_001910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3363602.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9240..9737
                     /locus_tag="ACHDLD_001911"
     CDS             9240..9737
                     /locus_tag="ACHDLD_001911"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN3363603.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2936 a         1933 c         1756 g         3555 t
ORIGIN      
        1 aaagagggat aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt ttaggtataa
       61 gtgccgtatt tattttgttc ggcgtacttt tttatactca gatcgctaca agcgaaaaag
      121 gattcaattt tgattggcag accacattat ttgtttctgt catttacttt gcccttctcc
      181 tagggatcgt tattgcttat ttcttattga aactattgaa aaaaatcgaa gataaccaag
      241 ctttttccac tcaaagctta aacatactaa aaaaaatcga acgaaccatt ttgtttattg
      301 gtttcgcctt ttttggctta cttcctaaat ttatgaaagt agcggattta gacgatgctc
      361 caggtttgat gttggtagga atcgctgtcg tattcattcc ttttgcagta tacacattaa
      421 caagcgtgct ggaaaaactg cttgaacatg cgattttact taagacagat catgatttga
      481 cagtgtgagg tgcgacatgg caattatcgt taatttagat gtagtattag ctcaaaaaaa
      541 aatgagtgtg actgaattga gtgagaaagt gggcattacg atggcaaaca tctctattct
      601 aaaaaatgga aaagcaaaag ccatccgttt ttccactctc gaaaaaatct gccaagtttt
      661 ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac tcgaatacta taccagaaga
      721 ataaaataaa gtagaacaaa cagtaaataa aaaataatct gaatgataga tgtgtgagac
      781 ttttagaagt tatttacgtc tattattcag attttttatt tcgattgaat accgttttat
      841 ctataaataa gatatgtact gactcataca aagggggcct tttcttctcc atgtactatc
      901 acttttcctg atacaactgg tttcctgtta cgttgtagtt ggatctgctt ttagtataat
      961 actttattca ttccaccaga ctgatagcag tttgagcagt ctaaataatc atgtagtatt
     1021 acaggccatt ctgtttcata ccttgaagtg cttaaaactt cagctaatgg cccattgtgt
     1081 taactcttac tccattcttt tttagtaagc tcccatatat actgtaaagc cttctatata
     1141 ttatgtcatg ttgcttttta acatttacta tgtagttttg ataaaatgat ttgaataaca
     1201 gtgatggaca attctttttt atttttaaca ttattttgat aataatgatt atttatttaa
     1261 aaatacatta gataacattt gttacgacac gaaaaagctt cacaaacaaa tatcgttatt
     1321 ttaaaatgag ggcgaactca cacaattagg aggaatagta tgccatttat tgtttacttt
     1381 tttctttcat tactcgtgat ctatattcca ttaccaacat ttttcctgct cgtcaatcgt
     1441 ctagcagagc aacatgatac gattgctgct cttacgaaac tttgtttact tctatttgtt
     1501 tgtctagatt actttacgag ctattattgg ctaaaacaga caagagtaaa aaagagatat
     1561 tatttcttcc ttgtcttttt caatctcttt gagtttctga tccattgctt tttacttcat
     1621 cagtatcata cgatccattt gtggatcatc tgggttttca aagtggtcgt attattttgt
     1681 atgttcacct ttcctatctc aagaaaattt agggaggcat tgttttatta aagaagcttc
     1741 tctgtatcaa taaagacaca tttaaggaaa aacttttaaa aatctaattg ggaatttgcc
     1801 cgcataaata agaggctgtg acaaaagtct tgtcacagcc tcttattccg tataaacagt
     1861 gttcaaaagc ttgcatcttc gataatatgc ccaaccaaat gaaaaatatg agaagctatt
     1921 tttgtttggc tgttcttatt acttgatgaa gaacagcttt ttcacgacct ctttttaaaa
     1981 gtctttttac agttttcgag caaagaatga tacgattagt actaaaatga tggcaccaat
     2041 caatgatggc acgattgcca ttcctgcaat acttggtccc caagaaccta ataatgcttg
     2101 accaagtgct gatccaacta aacctgctac gacatttgca ataatcccca tcgaagaacc
     2161 tttattcgtg attgctcctg caattgctcc gataattcct ccgacgataa gtgaccaaat
     2221 aaatcccatg tttcgttcct cctaatgttt tacttcctta ttactgaacc cgtggttctg
     2281 tattttcttt tactttttgt gcgccgcttc cagctgctga ttttacttta tctgtttgat
     2341 tcgaagcaaa ttcaccggtt tttttcgctg cgtcagttac tttgtcttgc accgtttctt
     2401 gatctttttc aaattcttct tttgttttga tgtccactac gttagcgttg acttcgatca
     2461 cttctaaatg tgtcatattt tttacttctt tgccgattac ttcttgcatt tcttcaaaga
     2521 ttttttcgat atctttgcca tattccacaa tgacatccat atcaactgct acttgttttt
     2581 taccgacttc tgtttcgatc ccagccgtta cattatctgt attcacaagt tttcctgcca
     2641 cattagagaa gaaaccaccg tttgcagata ataaaccgtc tacttgttca attgcgattc
     2701 cgatgatttt ttgcacaact ttatcttcat aattcagctc accgcgcatt gaatcttcct
     2761 gttcagaatc atgctttcct tggttatttg tatgatcaga accaagtttt ccttgtccgt
     2821 ttgtgtggtc agaactaagt ttttcttgtg ctttcgcatg gtcagaagga tgtttttgct
     2881 cttccacagg tttagaagga tcgattgcat catgttgttc tggaacttta gattctggag
     2941 ttcctggatg atgaggtgca tgcttttctg catcatgttt cttctcttct actccttgtt
     3001 gaacgttagg gactactgga tctggatgtg cagatactgg tgctgttttg ttcttttcat
     3061 cgttcacacc atttgattgg tttggtgtcg ttgggtctgg atgtgcagac actggttttt
     3121 ggttgttcat tttattgtta ttatttttgt tattgttatt catggatgtt tcctcctaag
     3181 atttaaaata gtgatctaag attttcgttt gtttcagata aaaaccgata gcgacgccga
     3241 tcgctgccat gatcaagatc aataaggtct tgaaaaagcc cacggtgatc cataaaacgg
     3301 ctaatatcaa tccaagtacc ccaaagatga tagggagctt gtatgtcgaa aatagttctt
     3361 tcatttttgg tcccccccta ttctactcgt ttgcggtcat tgcttcgttt agctggttct
     3421 tcaaagcctg tgtatttcac cgatactttc actggctctt ttgttcccaa tatgtgttgt
     3481 acttcatttt cgatttcttt catcaatgtt cctgttttac caatgagtgc tgaagttcgt
     3541 ttcaactccc cttttacatt gacggcgatc ttgtttttcg ttgctttgac atttactttt
     3601 ggtgaatgga tgaattcatc ttcatgcaag tgagaacgaa ccattccttc aattgctttt
     3661 ttatccaacg tcaactcccc atcaccatgt ttcagtagaa agtttccgat tgtttttgga
     3721 taaaacagga aaacaagtaa cgtgataatt gtcaaaatag caaatgcaac ggcaagccaa
     3781 aatacatatt gttgcacgta tgaattagtg atggaaaaga atcgaaactt ctctagctga
     3841 aaaggcaatg gataggttgc acttaatagt atcgcaaaca aaagcacaga tagcaaaagt
     3901 aatgttccga tcactccgat cgtttttaca cctttattca ttttcgcacc tcctcatagg
     3961 ggtgaaacga tcaacttctt ataagttgat cgcttctcca ccagttactc cgtaaatttg
     4021 ggctgtaaca taacttgctt tattcgatgc taagaaaaca taaactggtg ctaattctcc
     4081 tggctgacct gcacgttcta gtaaactttg ctgaccaaat tcagggatcg aatcagaagg
     4141 ttgtccttcg tctagttgta atggtgtcca gattggacca ggtgctacac catttacacg
     4201 gattccttta ggtgcaagct gttttgctaa accgatcgta aagttagcga ttgaagcttt
     4261 tgttgctgcg taatctaata aatgttcgct tggattgaat gcttgaacag aagttgttgt
     4321 tacgattgct cctcctgctg ggatatgcgg aacagcttct ttcactaaag caaacatagc
     4381 gatgatgttt atcataaatg tgtctttgac ttgctcaagt ggtaactcag caatagactg
     4441 acaagaaatt tgctgtcctg cgtttagtac catcgtatct agcccgccaa gttcactcac
     4501 ggctttatct atgatttctt taggtgcttg ttcgtcacga agatcaaaag gtagtaagac
     4561 aatcttacgt cctgcatctt tcacgtattt tgctacttcc tctgcatctt tttcttctcc
     4621 tgggaagaat tgaagagcga tatctgcccc ttctctagca aatgcaatcg ctgctgcacg
     4681 accgatccct gaatctccac cagtgattag tacacggcgg ttttctagtt gattattgcc
     4741 ttcataggat tcttctccac aatcaggaac aggaatcata ttctgctgta aagctggtgt
     4801 atcctgatct tgtttaggaa atttctccgt atggtacaag tttcgcgggt cgattaattt
     4861 tggttctgtc attgctgtat tcctcctagt ttttaaattg tattgccaaa acaaatttct
     4921 ttgtttgata cctgttttaa atagattctt taatttgtat aatctttccg gtataaatgg
     4981 taacctaaaa ctactgctgc tatcaaagag ccaaagacta cgagtataaa gcctagagca
     5041 gaacttttac ccggtaatcc tcctgtattc attccccaaa tccctgcgaa caacgccgga
     5101 acggaaatga ttaatgcagc tgaatctaga tatttcatga tatgatttag attattgtcc
     5161 atcatcccgt tgaatagatc accaatactc tctaataaat ctcgatagat ctcgatcatt
     5221 ttttctgttt gtcgctgtct caaatggaca tcatagagta gctgttcatc gtctaaacgt
     5281 ttgatgaaag tttcagaatt ccataattga tccattgttt cccgttgatc tcttaaggta
     5341 tgatcgatat atacgatgtc tctttcaaga tctgcctgtt tatacagttc ttcattttcc
     5401 gttgttttac gagctgcttt atccaaagcg tcaattcgct ttttcatctc tcttagttct
     5461 ttcacgtaat gggtgtaaat atctaataat gtatacgtga tgatcttttc aaacgagttt
     5521 aaatgatttc catgctttgt cagcagccga tccacaaatt cggtttttct atcgatacaa
     5581 gtaatcaaga gattatcgga aagtacaaaa gatattggcg ttaatttttt ttcgatacga
     5641 ttatctgact gatctagatt aatcactacc aaagaaaatg gatgctctag tttcgtctca
     5701 taaaggcgct ccatccgaga aacttcttcc ggatattcgg ttcctacgaa aatatcatca
     5761 ggaagctcga attcttcgat tgcttgctca atctcttcgg gctttgcact atcaagtacg
     5821 agccaattat attttttttc atctgtttct attacattct tttcttcatc cacaaagtag
     5881 tatctcaatg tatacctcct ttttggactt tctcgaaact attagctgaa gaagcagttc
     5941 tttaatttga tattataatc gagtactgcg atttcatttt ctaatgaaaa gctgtcaagc
     6001 tgtttgttga agtccatgtt caatcgttta ccgaaataat tttgctctaa tttagatgta
     6061 tagatgtcca ataatggatg ctcgatttta gtcgcatatt ctccaaattc cgaaacaaaa
     6121 ggaatgattt gaaaaaagct gccttttgca aaagcacctt tgtcccttag aaatggatcg
     6181 gaatcattga taagcgcgag tgcgattttt gtattttccc caaccttttc aattaacgtc
     6241 tctactgcat ttttagacat tgtaatttcc attcctactc cccctcttca gttgttgtct
     6301 tctattattt aacaactaca tctgtttttg gttgaacttt tgcatatttt ggatatttag
     6361 ggaatttagc tacacaacgg aaagccgtca tccctgcata tttttcgtaa tatttcttcg
     6421 ttggcccttc aaaatccttg ctccacggtt tcacatgtcc tgaaaaatgg ataatgtaag
     6481 gattgttgcg agtttcttcg tattcacgtt cacctgctgc tgttggatgt ttttttgctt
     6541 ttgccatgat atagccttgt gcattccaac gcggatgaag tggtaaccaa cggtcatgta
     6601 agatagcatt taatgcatct tggtcatgga atcgtaattt ttctggatta tgttcgatga
     6661 aatctaatac tttttgcgta atattttcat ctaaccattt tttcacattg atcagcatca
     6721 aacctgagtt gaaataacgc attgatttag ctgaaatttt cattttttcc agtcgttgat
     6781 ggaacccagc gtcttcaaca gctgctacga ttgaatcacc gaagtcaaga cgccataatt
     6841 tagagatatc ttgtaaagcg atcatgtcgc aatccatata taagatacgt tcaacttctg
     6901 tgccgcggaa caattccgga atcgcaatac gataataagc tgtttctgga atacgatcgc
     6961 tgattaccac atttgtaaag aattctttat tgattttcaa gaattccaca tctgagttca
     7021 ttcgtgcgtt tttcactgag aagcgtaaaa gctgtttact ttcaaaatcg atatcatcgt
     7081 caatcacata gaaccgaaca aatttagacg ggttgcaatt atctaatact gagacaaata
     7141 atgcagctaa atgtggtacg aattttgtgt tacaacttgc gactactgct atttcttttt
     7201 tattcattga tctcgcctac tttcgttaga gtactgtgtg ctaatttttt taagtataaa
     7261 ttggtatacg gatgatcttt caacgtattc caaggtttat catgtcctgt aaagtgaacg
     7321 atcgaaggtt tttcatttcc ttctttatac tgacgttcat atttttcatt tggtgctgga
     7381 tgacgttcaa aaatcagcga agtctgcatg ttccattttg gatgaagttg ttcccaatct
     7441 tcataaagta cagcattcaa agcatcttgg tcgtgataga tgattcgatc gccattttca
     7501 ctgaggtaat ggatagtttt ttctgtaatt tcttttttat tccattgatc aatgtcaatc
     7561 accatcactc ctgaattgaa gtacaagtca tcggaatcga ttcctaatct ttccaaagct
     7621 ttcacttggc ctggatcgat aatagcgccg attgtttttc catttaacgg ctcatcatat
     7681 agttttacga tatcgtctag tactaacaca tctgaatcta agtaaaggac tttcttataa
     7741 tcctctttag ctaataagtt tggtaaagaa attcttaagt aagcggtagt tgtgatatgg
     7801 tcactgacta ggaagtcttc gtagatatct ttttccactg tcaggaattg gatacttgca
     7861 ttacttgaat atttatttac cgtttcttcc aatccttctt tactatattc tgatagacca
     7921 tcgtcaatca cataaaattt tatgcgtctt gttttattgc aattttctaa tgcagttgcg
     7981 atcatcacgc tcaagtaagg agcataattt tcgtctgaag cagttaccac aggaactacg
     8041 ccgtatctct tctccatcaa aatcactcct ttttctaatc tttttctaac ctgttctaca
     8101 tctagaatat tcctcatttg gttttaaagc aaaatatatg ctttgttttc attgaatatt
     8161 ttaaaatttt tttatcacaa aacgcatcgg taagcgctaa ctattcgttt ttcatggctt
     8221 tttattcaaa ggtattgacc aaaaactact atatatagcc acctttttct taagaatgtt
     8281 ttttgaaaaa attcataatg acaaagcaag ttagtttcat ttttttaaat tctcatttaa
     8341 tcttaaggta acaggaaaat agatatgtaa aaagaaagta ggaagataaa atgaaactag
     8401 cagcgattga tttagacggt accttgcttg atcgaaatgg aaagatacca gaagaaaata
     8461 tccaagcact tcggaaattt gatgaaaatg gcggaatcgt aaccgtcgct accggaagga
     8521 acagcatttc tgctaaagat attttttccg aattaggaat cggtggctat ttgatttctt
     8581 ccaatggttc actgattgct gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt
     8641 caaaaataga tagcgataca ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat
     8701 ccatcattgc tagccgagaa acccaagatg atcagctgac attcgatgaa tcaaatctag
     8761 tcaaagatga cccttattat gaacatttca atttacagaa ccattctttt gatcaaattt
     8821 taaatatgtt ggaagatcct tctcttagct atcttaaatt agcattaaca gataagagtg
     8881 aagaaaaact acagaagcta caaagagatc tgcaagcaga tggtatccaa tcagtattct
     8941 ctgatccgca tttcttagaa atcacgccta caggtatcac taaagctcat tcacttgttt
     9001 ttctggcgga ttaccttgga atcgaatcga gtgacatcat ggcttttggc gatcaagaaa
     9061 acgacattgc tatgttgaat tacagcgggt tgagtattgc gatggggaat gcacagcgac
     9121 aagtaaaaga aattgcaaac caagtaacac tgacgaatga agaagctggt gttgcttata
     9181 ttttaaaaaa cttctattaa aaagaataaa atgacgatat gaatgtagga ggaaagaaaa
     9241 tgttagttat tattgatatg caaaatcata ttcttgatcc aaacagcgaa ttttatattc
     9301 cagagtcaga tcaattggca gaacgtattg ctcaaagatt agctaaagca cgccaaaata
     9361 atgaatatat tctcttcaca cgggatatac cagttgaact gaaggacaaa gaagaagaac
     9421 gagaagattt gcaattgatc cctcttcttt ctccacaaga aaatgaacga gtgattaaaa
     9481 agtattactt tacgattcca cctgaaactt tagctgaaat ccaggaaact ttatttgaaa
     9541 gcaaagaaga acaaaaagag atcgaagtag tcgggattga aacaaacttg tgtgtactct
     9601 ctaatctgat aggcctgcag agtgcttttc ctgaagcaga cttttttgtt gatccaatgt
     9661 tagtctccag tagaaaacac ggagaatccg ctttagagct gttgaaagat ttcaacgtct
     9721 ctatcgtcga atcataaaaa tatctatctt catttacgat atttaacaca caataaaaaa
     9781 agcacgcaga actgttttag tcctacgtgc tttttctatc aaagtagatt ggctttttta
     9841 tgacacagag tgaaacggca ggctcacgac ctctttctta aatggtgttc aatcagctga
     9901 ccctcggtat tagcttaaca aacggcaaaa tatgaggaac tatttttccg tttgttaagc
     9961 taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtat tcaaaagctt
    10021 gcatctgcag taataagaac agccaagtaa aaacataaga agctgttttt acttggctgt
    10081 tcttattact tgatgcaggg cagctttttc acaacctctt atgtaccttg taggactcga
    10141 acctacgacc ggacggttat gagccgtctg ctctaaccaa
//
LOCUS       DAZAOG010000092        10152 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000092 DAZAOG010000000
VERSION     DAZAOG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.092"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..1192)
                     /locus_tag="ACHDLD_001912"
     CDS             complement(173..1192)
                     /locus_tag="ACHDLD_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN3363604.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(1211..2212)
                     /locus_tag="ACHDLD_001913"
     CDS             complement(1211..2212)
                     /locus_tag="ACHDLD_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN3363605.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(2489..3637)
                     /gene="nagA"
                     /locus_tag="ACHDLD_001914"
     CDS             complement(2489..3637)
                     /gene="nagA"
                     /locus_tag="ACHDLD_001914"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN3363606.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(3713..4549)
                     /locus_tag="ACHDLD_001915"
     CDS             complement(3713..4549)
                     /locus_tag="ACHDLD_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363607.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(4733..5950)
                     /locus_tag="ACHDLD_001916"
     CDS             complement(4733..5950)
                     /locus_tag="ACHDLD_001916"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3363608.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(6099..6260)
                     /locus_tag="ACHDLD_001917"
     CDS             complement(6099..6260)
                     /locus_tag="ACHDLD_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363609.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            6333..6416
                     /locus_tag="ACHDLD_001918"
     tRNA            6333..6416
                     /locus_tag="ACHDLD_001918"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6367..6369,aa:Leu,seq:gag)
     gene            complement(6656..8026)
                     /locus_tag="ACHDLD_001919"
     CDS             complement(6656..8026)
                     /locus_tag="ACHDLD_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN3363610.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            8119..8468
                     /locus_tag="ACHDLD_001920"
                     /pseudo
     CDS             8119..8468
                     /locus_tag="ACHDLD_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8740..9030)
                     /locus_tag="ACHDLD_001921"
     CDS             complement(8740..9030)
                     /locus_tag="ACHDLD_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363611.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(9238..9930)
                     /locus_tag="ACHDLD_001922"
     CDS             complement(9238..9930)
                     /locus_tag="ACHDLD_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3363612.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         3128 a         2036 c         1591 g         3397 t
ORIGIN      
        1 aaatcggcca gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
       61 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
      121 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
      181 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
      241 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
      301 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
      361 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
      421 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
      481 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
      541 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
      601 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
      661 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
      721 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
      781 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
      841 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
      901 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
      961 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     1021 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     1081 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     1141 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     1201 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     1261 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     1321 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     1381 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     1441 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     1501 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     1561 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     1621 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     1681 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     1741 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     1801 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     1861 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     1921 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     1981 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     2041 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     2101 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     2161 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     2221 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     2281 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     2341 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     2401 aacttatgta aagtgatttt tactagaaca caaaaaaacc ttaaaaccaa acttaagaag
     2461 tttagtttta aggttaactt aaattatatt atgcgtggaa acgttccacg ccatctagat
     2521 aagttgcttc caatgtcata tcagggttca atacgataaa gtctgcatcg cgtccttgag
     2581 cgatttttcc acatgtatca tcgattttac agctgattgc tggaatcaga ctagccatca
     2641 tgactgcttg ttctggtgtt gctaatcccc agtctacaac atttttaatt gcttctttca
     2701 atttcaatat acttccagct aagttgccag attctagacg tgctgttcct tcttttacta
     2761 ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat cccaccggcc atcatacagt
     2821 ctgtaattaa tgcgacatga tcatgtcctg ctttttccat taggattccc gcagcatttg
     2881 gatgtacatg atggccatca caaatcaatt cagaaaagac atgttctaaa gataataacg
     2941 cacccaccat acctggttcg cgatgattca atccacgcat accattgaaa gcatggacaa
     3001 atacacttgc gcctgcttca acagcttttt gcgcttcttc tagtgtagca ttactgtgtc
     3061 ctagagaaac aactactcct tcttcagtca cttgtttgac aaactcttct acaccttgac
     3121 gttccggtgc taaagcaatt tttttgatta aaccacctga agcttcttgc cattcatgga
     3181 aagtatccaa atcaggatcc ccgaaataac ttgggttttg tgcccctttg tgttcttctg
     3241 tgaagaaagg accttcaaaa tagattcctt tgattttagc tcctggtgct tcctgataca
     3301 ttttgccgat tgtctcagct acatctttta aacgttcttt gcttgacgtc aatgttgttg
     3361 gtaaaaatga agtcactcca caagataaca gaccttcaga catcactttg atgccttctg
     3421 catcattatc cattacatca tgattcatat agccgtgaat atgtgtatct accaaaccag
     3481 gagcaatcca tttgccgctt tggtcaatga tgttgacttc tccctcaggt agttctttgg
     3541 tgtattcacc aaaaagtcca tcggtaattt caagatagcc tgcatgtttg acacctgacg
     3601 ctaaaaagaa tttgtcagca tagataaaag ttctcattct attctctccc tttgtttttc
     3661 tacctctaaa gtactcttat ttagagaaga agtcaagaat ggtctacacc atttacaaat
     3721 tttttgaaat ttgtttcaca gcttgtgcta agccttcata gaatgctgct ctttctggtt
     3781 cttctttacg cttgtatatt aagcttttaa gcacaaataa atcattgagg taatagtagc
     3841 tattcttctc ttgagcccaa tagattcctt gattcacctg ttctaacgct tcgtcgattt
     3901 cattttgatg gaataagaaa tttccataat tataaaaaat ttgcgaaaat tcactttttg
     3961 atcgttcatc agctgattga tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt
     4021 cagccgtttt tcctgccaca ccatatattt ttccgataca actaatcagt tgtatttctg
     4081 tgtcagaaac atggattctt tctttttggc tagagtagct taatgcttct ttcaaataat
     4141 aaagggactg agagatattt tgacgcaacg tgaattcgca aacaccgaga taataataat
     4201 agcgttgaaa gtcagttgct tgatacagat tttcagaaat atcagaagat tttagtactt
     4261 gttctaactt ccgataatca cgtttttgga aacaactatc caacagttca aataatttat
     4321 cgtttgtccg agtagttttt cgagagagat tcatcacatg atcaatcgtt acccctagcc
     4381 gttgacaaat ctgcatcatc accaatacat tcggcagttc ctcattattt tcgatccgac
     4441 ttaatacact ttgtgaacaa atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt
     4501 ttcgaatctc tttaatgatc gttccaaagg actcgattaa cgtttccata agtatcctcc
     4561 ccaaaatatg cgtattcgca ttctattcgt attttacttt aggaagtcgc aaacttcaag
     4621 aaaagacttg caactaaata atatacttta gttatttaag aaaactccac tagaaagaga
     4681 gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt tggtttttaa aatcattttt
     4741 ggcgataggt tgctaggaat tctctcattt tcttagctgt ttgtttcaaa tcatccattt
     4801 taggaagata aacgattcga aaatgatctg gctggttcca attaaatcca ccaccatgta
     4861 caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat
     4921 tgaatcgctt tgtatcaatt tttgggaata tataaaaagc agcttttggt tttacggctg
     4981 aaagaccagg aatatcattg attgcattat agatatattc tctttgttca tagatccttc
     5041 cgcctggcag gagcagctca tcgacacttt gataaccgcc tagcgcggtt tggataattt
     5101 gctgagacaa tacattggaa cacaaacgca tggaggacaa catattcagc ccttctatat
     5161 agcctttgac gtgtgactta tctccactta ggaccatcca gcctactcga aatcctgcta
     5221 cacgatgaga tttggataga ccgttcagcg taacaacgaa caaatcaggc gctaatgtag
     5281 caatcggaat atgttctaga ccatccatca ctaagcgatc atagatttcg tcggaataaa
     5341 taatcaggtt attttgtcga gctacttcaa cgatttcttc taataattct tttggataaa
     5401 gtgccccagt aggattatta ggattaatga taacaatagc ttttgtccga ctcgttattt
     5461 ttgatttgat atcatcaata tcaggatacc attctgcctc ttcatcgcaa atataatgaa
     5521 caggtgtgcc tccagctaat gcaatagaag ccgtccacaa cggatagtcc ggcattggaa
     5581 ccagtacttc atctccattg tccagcaacc cctgcataca catcgtgatc aattcgctca
     5641 ctccgttgcc ggtgtagata tcattgatcg tcacattcgg aaaatgtttc agttggcaat
     5701 attgctcaat tgcttttcta gcagaaaaga tccccttgga atctgagtag ccttctgagt
     5761 cacgggcatt catgatcaaa tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg
     5821 cgggatttcc cgtgttcaat tttaaaatac taatgccctc ttcttgcatc cgctccgctt
     5881 cttctagaac gggaccgcga acatcgtaac tgacaccttc taatttgttg gatttttcaa
     5941 attctctcat cacttagcca atccctcatt tcaaaaagtt agaactttcc aaattttttg
     6001 ttatttacta tttcaatatc atactccatc ctagaaaatt ttgccactta tttaggaaaa
     6061 acgtaagtat taaaaaaaca caaacaagcg ttgagaactt aattgtcatg atagtcattc
     6121 catagattcg ctgcccaatc cattgcaatt ccacaaatga ttcctagcat aaaaccaagc
     6181 aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga tcattcccag tcctgaaaat
     6241 tctccgtcat atttctccat cttaataagt cctcccattt ttgagcttga caactatgta
     6301 aaacctcgct atcattagtg tgttattatt acgcagatgt gttggaattg gcagacaagc
     6361 atgattgagg gtcatgtgag cgttagctcg tgtgggttcg agtcccatca tctgcactac
     6421 aactcgtgaa gttaattttg cttcacgagt ttttttgttt gaacgtttct ttatgccatt
     6481 atatacatat cactgtatcc tttacaaaaa gaacgtacaa tcatgtaaac agcctgaggt
     6541 gagtatatga aggtcggaaa caagctactc gtagaaactt gcaagtgtta aaacaattaa
     6601 tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga gggatttttt gtatattatc
     6661 tgtttatcac cgttattata aaggtacaaa taccaagcac ggttaatcca aattgcataa
     6721 attcattttt ttgtacactc atgcttttca ttagctcttt aactgccatt agtactaaga
     6781 tggcgataac aacataaagt atatagtacg tccgcattcc ggaagcccat actgtgggag
     6841 aaaatcccat aattaacctt gaacaaaatc ccattatcat caaaataact aaaaaaatag
     6901 aagttttctt attgtcaaaa cttaaataaa ttccaacaat taaacatagt aataatgcca
     6961 agactactaa agtagctatc caagtacctg gataaaaaag tgacagtttc gttcctaact
     7021 ctgtaaagag attattcagg ttactactac tccaaatcat tgccctttta tttccattga
     7081 cgtaagtaaa gctttgaccc attgtattac caaaataaat aatcaggttt aagaaaaacg
     7141 gtaatgcagt caatatacgc acataatagt tttgacattt cctatatgtt agaaggaaaa
     7201 ttaagaaaaa tagcaataaa aataagatat tcatgtctaa aaaaaacggc tttccaaatg
     7261 atgaaatacc taaatctaac ttattaagaa agttgaaatt tttatattca ggaaaccatt
     7321 tattaatttc atgtgcagca cgtaatgcat tccctggagt tgtcaaagag aaaattaatt
     7381 ctgctaagct tattattgaa aatactgata acttataatt atatcgtcca ttgacaatca
     7441 aatataaact aactatactt gttagaacaa aaaaacaggc gttgacttgt tcttgattag
     7501 ttaaaaatat caataaagga atacttaccc aatatatttt tctatttatt tcttctcctc
     7561 ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa tgctactggc cactgatagt
     7621 tcaaggtggt tgctctccaa ccggtttccc ccattactgt caatggtatt aaacagtata
     7681 tagagcaaac caataataaa tttttaacat ttattttctc aaaaacatac ttgcaaagta
     7741 atactacaat tcctaacata atcgtagaat ttaataattt ccatagcata aagtgtttag
     7801 aaaagaacat taagaatagt tctataataa ttcttgaact ccatgtctta taatgttcta
     7861 tcgaagcacc caaatagcca agttcatcta atctacttgc ataaactacg tcatccgctc
     7921 tcatagtcgg catatacata ttaaagagta aaaagatacc ccaaatccct attagtaaca
     7981 agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt atactccttc
     8041 ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata acttaaaagt
     8101 tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat ctacgatagt
     8161 caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac ctatggattg
     8221 cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt gcttttaaaa
     8281 ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg
     8341 aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac gactatgata
     8401 atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc tacaattcca
     8461 ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc cattgagcga
     8521 atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct caaatgagtt
     8581 tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata tagattgagg
     8641 ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca tctttccaaa
     8701 acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc tttcatcttg
     8761 tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt tgggtttccc
     8821 taacacatga taggatctca caacaaattg ctttctatta ttataataac ctgtaacgac
     8881 taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag caatcaaaca
     8941 attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca aagtacggat
     9001 cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat tatacgaaca
     9061 tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg tctattacct
     9121 ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa aaaaaagagg
     9181 cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta agaaagatta
     9241 tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt ccacttcatc
     9301 ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag cagttgcttc
     9361 gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt cttgtgtcag
     9421 tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa gttttgcttg
     9481 tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat catttttttg
     9541 gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt gtgcatcctt
     9601 gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt tttggttttc
     9661 taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt gcaatgtctg
     9721 ttctttttgt gctagtcgag cggtaagatc tgtaagcgct tgctctttat ctgtcaatgt
     9781 atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc caccccacaa
     9841 aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa atgcaacgac
     9901 aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca atacttttac
     9961 atgctaaaag taagtgatct caaacacaat gtcaagcgtt tttaaattac atgtgtaatt
    10021 ttaatataaa tactatcaaa tactcattta atcctccttt tttatctatt taaaaaatct
    10081 tttttataga gaaaaatgca ggttaatact ttcttaaata aatatttatg gtgaacaaaa
    10141 acaaaaaagc aa
//
LOCUS       DAZAOG010000093        10115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000093 DAZAOG010000000
VERSION     DAZAOG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.093"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..625
                     /locus_tag="ACHDLD_001923"
     CDS             119..625
                     /locus_tag="ACHDLD_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3363613.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="ACHDLD_001924"
     CDS             642..1541
                     /locus_tag="ACHDLD_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN3363614.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="ACHDLD_001925"
     CDS             1628..2512
                     /locus_tag="ACHDLD_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN3363615.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="ACHDLD_001926"
     tRNA            complement(2704..2775)
                     /locus_tag="ACHDLD_001926"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="ACHDLD_001927"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="ACHDLD_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN3363616.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="ACHDLD_001928"
     CDS             4318..5409
                     /locus_tag="ACHDLD_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN3363617.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="ACHDLD_001929"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="ACHDLD_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN3363618.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /gene="pgk"
                     /locus_tag="ACHDLD_001930"
     CDS             6561..7751
                     /gene="pgk"
                     /locus_tag="ACHDLD_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN3363619.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /gene="tpiA"
                     /locus_tag="ACHDLD_001931"
     CDS             7855..8610
                     /gene="tpiA"
                     /locus_tag="ACHDLD_001931"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN3363620.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="ACHDLD_001932"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="ACHDLD_001932"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN3363621.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3541 a         1871 c         2095 g         2608 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaat
//
LOCUS       DAZAOG010000094        10084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000094 DAZAOG010000000
VERSION     DAZAOG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.094"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHDLD_001933"
     CDS             162..392
                     /locus_tag="ACHDLD_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN3363622.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHDLD_001934"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHDLD_001934"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN3363623.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHDLD_001935"
     CDS             1803..1979
                     /locus_tag="ACHDLD_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN3363624.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHDLD_001936"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHDLD_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN3363625.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACHDLD_001937"
     CDS             2474..2941
                     /locus_tag="ACHDLD_001937"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363626.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACHDLD_001938"
     CDS             3026..3910
                     /locus_tag="ACHDLD_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3363627.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACHDLD_001939"
     CDS             4024..4260
                     /locus_tag="ACHDLD_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN3363628.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACHDLD_001940"
     tRNA            4348..4428
                     /locus_tag="ACHDLD_001940"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACHDLD_001941"
     tRNA            4435..4506
                     /locus_tag="ACHDLD_001941"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACHDLD_001942"
     CDS             4631..4801
                     /locus_tag="ACHDLD_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363629.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACHDLD_001943"
     CDS             5021..5728
                     /locus_tag="ACHDLD_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3363630.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACHDLD_001944"
     CDS             complement(5788..6432)
                     /locus_tag="ACHDLD_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN3363631.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACHDLD_001945"
     CDS             6559..6846
                     /locus_tag="ACHDLD_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363632.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACHDLD_001946"
     CDS             6954..7274
                     /locus_tag="ACHDLD_001946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3363633.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACHDLD_001947"
     CDS             7355..8668
                     /locus_tag="ACHDLD_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN3363634.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACHDLD_001948"
     CDS             8665..9384
                     /locus_tag="ACHDLD_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN3363635.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACHDLD_001949"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACHDLD_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN3363636.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DAZAOG010000095        10059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000095 DAZAOG010000000
VERSION     DAZAOG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.095"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..2060
                     /gene="tdc"
                     /locus_tag="ACHDLD_001950"
     CDS             225..2060
                     /gene="tdc"
                     /locus_tag="ACHDLD_001950"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN3363637.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2365..3843
                     /locus_tag="ACHDLD_001951"
     CDS             2365..3843
                     /locus_tag="ACHDLD_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3363638.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3809..5140
                     /locus_tag="ACHDLD_001952"
     CDS             3809..5140
                     /locus_tag="ACHDLD_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3363639.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5254..7806
                     /locus_tag="ACHDLD_001953"
     CDS             5254..7806
                     /locus_tag="ACHDLD_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN3363640.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7813..9252)
                     /locus_tag="ACHDLD_001954"
     CDS             complement(7813..9252)
                     /locus_tag="ACHDLD_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363641.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9227..9409
                     /locus_tag="ACHDLD_001955"
     CDS             9227..9409
                     /locus_tag="ACHDLD_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363642.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9598..9960
                     /locus_tag="ACHDLD_001956"
     CDS             9598..9960
                     /locus_tag="ACHDLD_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN3363643.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3203 a         1654 c         2134 g         3068 t
ORIGIN      
        1 tttaggttat ctattttaga agactgattt attgaaaaat tgttcgcttt tttgcgttga
       61 attatcagaa aaatccgact ttatttattt ttgtttgtaa aaatctacgt ggatttgatg
      121 ttataaaaaa aaatttctca tttttggcgc tatagtgaag aggtgttaca gataaacaca
      181 tgtatctgca agacattgac caacaaaata tggagggaag aaaaatgaaa gatatggata
      241 tcaaggccgt ctttataggc gacaaagctg aaaatggacc agtttataaa atgttgttga
      301 acaagatggt agatgaacat cttggatgga gagaaaatta tattccttca gatatgcctg
      361 caattagcga aggagataag ctcactccag attatctagc cacccgcgac catatgatag
      421 aggtcttgga tgaagtcagt cagcgattac gcgcaggatc gatcccatgg cattcagcag
      481 gccgttactg gggacaaatg aatgctgaaa cattgatgcc tgctttatta gcttacaact
      541 atgcgatgct gtggaaccca aataatgttg cattggaatc ttctatggca acttcgcaaa
      601 tggaagcaga agtaggacag gattttgctt cattattcaa tatgacagac ggatggggac
      661 acattgcagc agatggttct attgctaatc ttgaaggatt atggtacgcc cgttgcatca
      721 aatcgattcc tttagctgtc aaggaagtgc tgcctgaaaa agtgaaaaaa atgtctgagt
      781 gggaactttt gaatttatct gttgaagaaa ttttggaaat gaccgaaagc tttacggacg
      841 aagaaatgga tgaggtcaaa gcagcttctt cccgtagcgg aaaaaatatc caaagattag
      901 ggaaatggct ggtccctcaa acaaaacact attcttggat gaaggcgtta gatatttgcg
      961 gagtcggctt agatcagatg gttgctatcc cagttcaaga ggattatcga atggatatta
     1021 atgctttgga aaaaacaatt cgtgaattag ctggacaaaa aatcccaatt ctaggtgtgg
     1081 ttgctgtcgt aggaacgaca gaagaaggtc aagtagacag tgtagataaa atcgtccaat
     1141 taagagaaag gttaaaagat gaagggatct atttctattt acatgttgat gccgcatatg
     1201 gcggctatgc gcgttcactg tttctaaacg aagcaggaga atttgtgccg tatgcttcct
     1261 tagctgaatt ctttgaagaa catcatgttt tccaccactg cgtaacgatt gacaaagagg
     1321 tatatgaagg gttcagagct atttcagaag cggattctgt cacgatagat cctcataaga
     1381 tgggctatgt cccttatgct gctggaggaa tcgtaatcaa acataaaaat atgagaaata
     1441 tcatttctta tttcgcgcca tatgtgtttg aaaaatcagt aaaggctcca gatatgttag
     1501 gagcttatat tttggaagga tcaaaagctg gagcaaccgc cgccgcagtc tggacagcac
     1561 atcgtgtatt gccattgaat gtgaccggat atggtcaatt gattggggct tcaatcgagg
     1621 cagctcaaag attcagagaa tttctcgatc atctgacttt tactgttaaa ggaaaaacaa
     1681 tcgaagtcta tccattgaat catcctgatt ttaatatggt caattgggta ttcaaagaac
     1741 aaggctgtac agatttgaac gctatcaatg aattaaatga aaaaatgttc gatcggtctt
     1801 cttatatgga tggcgatgtt tatggtgaac ggtttattac ttctcatact acatttacac
     1861 aagaagatta cggtgattct ccgattcgtt ttgttgaaag aatgggtcta acgaaagaag
     1921 aatggaaaaa agaacagaaa atcactctgc tacgtgcggc tatcatgact ccttatttga
     1981 atgatgatcg aattttcaat ttttatacaa aaaaaatcgc caaagcaatg gaaaagaaac
     2041 taaacgaaat catccaatag aactgatttt tgtgtgaaag aataacaaga gacatatgga
     2101 gaacagacaa ttatttgctt catcaaaaca attgtatttc cccctgtttt tcatatattc
     2161 ctcgccaaga tattcttttt tataaaacgt tctataagtt ggatcgttcg tttcatgtat
     2221 caaacccagt tcactaagat cgttcaatgg atgtttcggt gatactgggt tttgtactga
     2281 atcgatgaaa ccttgtcatt tctgttcttg aacaaaaata aaagaaaaca aaaacatatg
     2341 tcgctattga aagaaggaat aaagatgaaa gatactggtt catcaaagca aatcacgacc
     2401 atgaccttta tcggaatgac ctgcgccctt gtagcaagtg tacgaaatat tccagatgta
     2461 gcagcaacga gctggacgat gattttttac atgctggttg cagtattatt gtacgctttt
     2521 ccaatctcat tgatctctgg tgaatttgct ggaatgtttc cccaaaaagg tggtccagaa
     2581 ctttgggtat cgaatgctct aggaaaaaaa tgggggttcg ttgtctcatg gctgttatgg
     2641 gtccaaatgt ttcctgggat ggtgatggtc gcttcagcac tcgctccgtt gtttggtaat
     2701 attatcgata atgtgccgct tggtttgaac agcaaattta cattagttgt cattttagtc
     2761 gtttattgga tcatcactct tttgaatttg aaatttgata tggcaaaaat cggcggaaaa
     2821 gtcggtgttt ggttaggatt gtacgtgcct ttgacgatga tgctcttatt agggtttgct
     2881 gcgtggatca aagttgggat cgtaccaacc ggaacattag gcagtttttc atgggacaaa
     2941 ttaataccag atactacgac tgcatcctca tttgtttatt ttgcgccgat catgtttatt
     3001 tttactggta tcgaaatgtc ctctgtgtat atcactcgat tggaaaatcc agttaaaacg
     3061 tatattcgcg gagtatttgc cgcgttaatt tttatatttt tcgtcaatat tttgaatgct
     3121 ttagttgtag caaatgtcgt accaaaagga caaatggaat tgaataatat cgcgcaatct
     3181 atttcgattt attgccaaat tcttggtttg ccacatctta ttgtgaatct gtttagttta
     3241 cttgtcttca ttggtgtagc agtccaatta tctgcttggg cctcaggacc agcaaagaca
     3301 gtgacagaaa gtgctagaaa aggtgcgtat ccaccaaaat tcaatttttg gaagacgaat
     3361 caatttgacg tatccaaatc cgttattttg actcagtctg tcattatctc aatctttgca
     3421 cttttttatt tgctgattcc tggcgtcaat caagcatttt tgatgctcgt aaattcaact
     3481 actgttatct actgtatcgt ctatgtcatc atggggatcg cagttttgag attgcgttat
     3541 acacatgcaa agttgaatcg accatttaga attggaaaaa aagagaaaaa gagcaatctt
     3601 ggagtttgga ctgtcgttat tgttttattt gcggcaatcg gattttctgt cggactgacg
     3661 atgaaagctg gaacatggat caatctaatt gcagtcacag ccatcagtgt cattttgttt
     3721 gtagtaccac tctgtattga aaaaataaag aaaccttctt gggaacagga agtcgccatg
     3781 ctgcttgaaa aagaaaaagg tgaacttgat ggaaaaaccg gcaatgaaac ggactctaac
     3841 taatcgaaat atccagttaa tcgcattagg tggcgcaatt ggaacaggat tatttttagg
     3901 ttcagcaaag gcaattgcat cagcagggcc agctgttatt ctcgtttatt tgatatgcgg
     3961 tttggctatt tatggtttga tgcgggccat gggtgattta tttttagcaa atagtacatt
     4021 taactctatc agtgatttcg ttgactatta tttaggcaaa aagtgggcat tttttgtcaa
     4081 ttggacttat tggctttgct ggcttggagc tggacttgcg gaattaacag caataggatt
     4141 gtatatccat ttctggtttg tacatgtacc agctttgata agcggaggta gcgcactaat
     4201 gatcttaatg gcagtcaacc ttgttgcagt caagtggttt ggtgaattgg aatctttttt
     4261 ctcaatcatc aagatcttgg ggattttcct ttttatctta ttgggtatag gattgagtgt
     4321 ctcagatttt catacccagt ctatcggaat caaagaaaat tttggacaga tcgatgaatt
     4381 actaccgttt ggctttgctg gaatggtagt ctcatttcct atggttttat tcagttttgc
     4441 tggaattgaa atgatcggct taaccgttgg cgagacaagt gatccgcaaa agaatttgaa
     4501 aaaagcaatc aactgtctgc cttggcgtat tctctttttc tatataggaa cgattttgtc
     4561 gattttattt gttgttccat ggcattgttt agatttaaaa gaaagtcctt tagtgactat
     4621 gctgcaagtg atccattgcc aaggcggagc agcaattgtc aatctaatga ttttggttgc
     4681 ttctttgtcc tcagcaaata gtgcagtcta cagtactagc aggattttat atcaaacagc
     4741 aaaagaaaaa agtttgcccc attggttttg tgcgctttct aagaaaggcg tacctttaca
     4801 cggtatttta attacaggaa tgttattttt tgccggtctt tgtctacatt tttttgtagc
     4861 tgatagtcga ttgttttttc agcttttggc aggcatgaca acgagttgtt ttctatttgt
     4921 gtggtcttcg atcttgtgtg cacatgttca atttattaaa gaaaagaaac ggaacactac
     4981 acaaagatat aaagatcttg ctttgttggt gttttttact tcaattgctt tcagcttgtt
     5041 attcgaaagc acgacacgct ttattccttt tttattggtt atctggtttg tattcttaaa
     5101 ttttgtatat aaaagaataa gcaatagaaa tatcagttag gcccagatac attttgcaaa
     5161 acgaattatt tcagaccccc ctgaaataat cgatacgcct gaaaaaactt ccctgcgtat
     5221 aataacagta tcaattaaac aggaggggat aggatgaact ggtacagatt gacggtagaa
     5281 gatgtattga aaaactatca aacatcaaca gaaggcttgt cgcaagaaga acgacagaaa
     5341 cgcctgactg atcaaggata taatgaaata aaagcaaggc agcagacaaa acgatggaaa
     5401 aaaatagcaa agcattttac tgatctgctg atgatcgtgt tgatcattgc ctcttttttg
     5461 aaatttgctt ctagtgaata tattgaagga agtattattt tattcgtagt gatcgtcaat
     5521 ggactcgtta gttactggca agaaagaaaa gcggaagaat cattgaatgg tttgaagcag
     5581 ctgatgggac aagaagcgat agtactatca aacgggatgc aagaaaaaat ccctgcgaaa
     5641 gaactggtaa aaggagatgt cattgtctta cgtgccggag atgtcgttcc agcagatatc
     5701 cgattaatcg aagcacatga tttattagtg gaagaatcga ttttgacggg agaatcagaa
     5761 gcatctgaaa aaagtcatgc cgttttgaca gaagaagaat cgatcggcga ccagaaaaat
     5821 atggcttttt caggaacgct tgtacaatct ggttctgcag tcggtgtcgt tgtagaaaca
     5881 ggaaacacga cagaaatcgg taaaatcaat caagcattgc aatcggtcga acaacaaacg
     5941 acgcctctga ttaaaaaaat caaccgatta aacaaacaaa tttttcaagg cattctttgt
     6001 ttgagcctat tcttagttat ttttacgaca tttcgatatg gtatggattg gcatatcctt
     6061 ctttcggcta cgattgcttt ggtggtgtca atggtacccg aaggattgcc agctgttttg
     6121 accatgattc tatccgttgg agtacatgaa atggcaaaag aaaaggcgat catcaaagga
     6181 cttccttcgg tagaaacatt aggttctatg acagtgatct gttcagataa gacaggaaca
     6241 ttgacaaaaa atgaaatgac agttgtggat gtagctgcaa aagaagaagc ctgcgtgttg
     6301 tccattatga aaaactgtca ggaattaaaa acaaaagaag aacagaaaac agaaaattta
     6361 agtggcaatc cgacagaagt tgcgctgatt cgacacacag aaacagccaa tctacctttg
     6421 aaaaaaatag aagcaaagat tccgttcagt tctgaataca aatatatggc aacgatgcat
     6481 gcggaggaaa aaggagcggt gatttacgtc aaaggtgcac cagaagtatt atttgctaaa
     6541 tcaactcttt cttctgcaga acaagaagca tggagtcaga cagctgcaga atttgcgaga
     6601 aaaggtcagc gtgttttagg atttgcttat aaaaaagtag attcgaaaca ggaactcacg
     6661 catgagactt taacccaatt aacttttgca gggatcgccg gattgatcga tccgccaaaa
     6721 gaaagtgcag tcaaagctgt aaaggaatgc caacaagcag ggatctcagt gaaaatgatt
     6781 acaggggatc acaaagacac agcaaaagcg attgcggaac aaattggctt aaaacatact
     6841 gccaaagttt tagaagggat cgatttggat cttatgtctg atgaagagtt gattcagcaa
     6901 gtgccaatag tcgatgtctt tgctcgaacg acaccagaac ataaactgcg aattgttaaa
     6961 gctttgcaga aaaatggaga aatcgtcgga atgaccggag acggcgtaaa tgatgcacct
     7021 gctttgaaaa gatccgatgt aggaattgct atgggaatca aaggtagtga agtaagcaaa
     7081 caggcagctg atatggtttt aggagatgat aatttccata cgattgcaaa agccgtaaaa
     7141 gagggccggc ggattttgga taatttgcaa aaaaccatca atttcttctt gccaactgca
     7201 ttagctcaag gattgatctt gatttgggca cttatgctga atcgcccgct acctttgtcc
     7261 ccagtacaga ttttatgggt caatatggta acgacaatca ctttatctta tgcactaggg
     7321 ttcgagtcgc cagatccaga aatcatgaag cgcccaccaa gagatccaaa gcaaggaatc
     7381 ctatctggtt atcatttgtt tcggatcatc tatgtctccc tactgatcat ggttcctgcc
     7441 tatttattgg ctatgcagtt tgaagggcaa agcttacagc aaaccattct tctgcaaaat
     7501 atcgtcttag ctcaagctgt ttacatgatc aattgcagag agttgtccaa gccgtcattg
     7561 aaccaaggtc ttttgcaaaa caaagcgctt tttctttcat taggtatcct tgctttattg
     7621 caagggatgg ttatttttct accagtcggc cagcagctga tcggtacagt aagtttaacg
     7681 atgacacaac agttattgat tggagcaaat gtgattcttc tattcttagt tgtagaaata
     7741 gaaaaaggga tcatgaacaa actgtttcgc aaaaaagaaa aaacagctag agaatacagt
     7801 cgctagccgt tactataaaa aagttttgaa agtgagttca gtcaaacgat tgagctcatt
     7861 tttcgttgga atagaagaaa tgtatacaat agggatatct agagaatcta gagggaaatt
     7921 ggaaatcaag atatctgttt tttcaaaaga aatagtggaa agatcggtta gttcgatagg
     7981 taagaagcag acacgttttc ctaagtaatc ttttaattcc tgctgcaaga acgccttcca
     8041 tgaaggttct ccttggaaaa taaaatagac attgaacaaa ttcggtttga tagaaaaaat
     8101 cgtttgttgg atcagtagag acaagagttg gagaaagtaa tttggttcat tgatttctat
     8161 ttcttttttt agatcgttca gtaaattctc tttctgttga tacaagtgtg catattgctg
     8221 atcatatgtt tctaaaaaac gatggtattg acggtccaat gcttcaatca atatcggcga
     8281 ttcatgatat tttaatatga ggagagccaa gttatcagtc agagatgttt tttttagttg
     8341 atcaagagac agctctgaag ctagttcctc tacaaacgtt tcgaggacct ctaaaaatgg
     8401 agaatgatgc aggcagcgag cggtttcttg tccatagtta ttgttgtaat tccaactttc
     8461 cagaaagcag aaagaagcaa aagaaagctc agtgtcttta agataaatac cttccgttaa
     8521 atacaatttt ttcaatgcag agtcatacag ggaggagagt gtgctgttgg tatggataga
     8581 agaatctaat cggcaattcg cttttatacg gatcgtattg ataaagaacc aacatgttcc
     8641 gaaaatctct tctgtttcaa ccgctagttc agtttgactg aggtttctta aaaattgaaa
     8701 ataatgggat tcatggatcg aataatcctc gaaaaaatag ttgttatgag tgaaaggaat
     8761 cagcaatcga tgataaaaag tacgaatcgc acgctctttt ccaagaatac aagctgtata
     8821 tttcgtcaaa tcgatctgca agtcgtaact tgctaaacgc tgattgattt tagtcacatg
     8881 tcttttcaat gtcccataag aaagcccatg gtcggctaga aaatcaggga cagcaacttt
     8941 ttttgtaaaa agcagatttt tcagcagttc gacactgact gattgtttga aaaaaatctc
     9001 ccaaaggtca tttgttaata aattttgctg gttttgcaaa ctgattcctg cttgatcgct
     9061 gatgatgtcc cagccttcgg gcagctgaga gcgaatagat tgaaggtcag aaaaaatcgt
     9121 tcgttcggtt gtccctatga tttcagctaa ttcatttacg gttatctttg tatgattcag
     9181 caattgttct actaaagaaa catgacgtcg aatatctttt tcggtaatga tttttttcat
     9241 tagtgaatac atcaatcaga ctcccttcta ttctaagaat acggagaaaa taaacggtgg
     9301 tcaagtttca actgattttt caatcattgt ttatttttct ggagcattta ctgatgataa
     9361 gaatttcatc atgaacttga aaaatgatac cactaaaatc aaaaaatagg tgggcaaaca
     9421 ataataaaca ttttaatagt agatggtagc aagaccaaca caggcggatt gaacatttaa
     9481 gagttgataa aaaagaaaaa tatcgaagta tttgattagc aactacttgt attcattttt
     9541 ctattcttac gtggtatctt tgaataaagc gaatcaataa gaaagagatg gaagcagatg
     9601 ctaacgaaaa aagaactaat cgatcacatc aatgaaacat acaatttgtc tccagaatat
     9661 atttttaaaa aatttcctga ttattgcgta ttcagacatc aaaaaagcaa aaaatggttt
     9721 ggggtcctga tgtctgttcc agaagaaaaa atctatgatg atgcaagtga ccataggatc
     9781 atcaatattc ttgatttgaa ggtcgatcct gaacttggcg gaattttgcg aggaaaagag
     9841 gggtattttc cagcctatca catgaataaa gaacattgga tctctattga tctacgtaaa
     9901 atagaaaagc tagatgaact agcagatttg attgatggaa gttttaaatc aacgcaatga
     9961 atgtctagga caatagagaa gaggttgtga cagaaatgtt tagcttcaag aaataagaag
    10021 gaatttacga aaattgcttc tcaaattttt gggaaattt
//
LOCUS       DAZAOG010000096         9961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000096 DAZAOG010000000
VERSION     DAZAOG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.096"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(181..2301)
                     /locus_tag="ACHDLD_001957"
     CDS             complement(181..2301)
                     /locus_tag="ACHDLD_001957"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3363644.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2531..3709
                     /locus_tag="ACHDLD_001958"
     CDS             2531..3709
                     /locus_tag="ACHDLD_001958"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN3363645.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3725..4483
                     /gene="aroD"
                     /locus_tag="ACHDLD_001959"
     CDS             3725..4483
                     /gene="aroD"
                     /locus_tag="ACHDLD_001959"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN3363646.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4506..4991
                     /gene="ybaK"
                     /locus_tag="ACHDLD_001960"
     CDS             4506..4991
                     /gene="ybaK"
                     /locus_tag="ACHDLD_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN3363647.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5062..6315)
                     /locus_tag="ACHDLD_001961"
     CDS             complement(5062..6315)
                     /locus_tag="ACHDLD_001961"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3363648.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6433..7782
                     /gene="gdhA"
                     /locus_tag="ACHDLD_001962"
     CDS             6433..7782
                     /gene="gdhA"
                     /locus_tag="ACHDLD_001962"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN3363649.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7894..9240
                     /locus_tag="ACHDLD_001963"
     CDS             7894..9240
                     /locus_tag="ACHDLD_001963"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN3363650.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9548..9934)
                     /locus_tag="ACHDLD_001964"
     CDS             complement(9548..9934)
                     /locus_tag="ACHDLD_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3363651.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3185 a         1802 c         2041 g         2933 t
ORIGIN      
        1 aagtatgaaa ttcctttagt gaaatcacga attaaaacca gaaaatattt tgtaaaaagc
       61 aagaggttgt gaagaagctg ccctattcta ttaccgtaga tgcaagcttt tgaacactgt
      121 ttattcgaaa agaggagcag cgacaataat tttgtcgctg ctccttttaa atttattttg
      181 tcattcggaa gaacttgtgg tcgttccgtg aagttcttgg tacgtttgcg ttttataaag
      241 atcaacagtt gattgtccgt tgtgttcact aaagacacta gttgacttgt caccaagaga
      301 tttttcagct tcttttaatt tctctaagcc attcttgtag tcataatcac tggtatcaat
      361 tgggtctaat ccactattcg tatagaaccg aagaagatca ccattattga tttggtcaga
      421 aacagctaac tgtttatcaa ctttttcttt cagtgaatca atttcattct gtacttcctc
      481 agtaggttca gtaatagctt caccagtttc gttactatag atcgtaccac cgtagtaagt
      541 atattttggt gtaacaaaat caccatcacg gaaagaaaca agctgttcat gatctttcga
      601 gaatagatct tgtcctaact gaatataatt ttttgtatct acacctagta agtgaagtaa
      661 tgtaggcaag gcatcaactt gtccgccgta agtgtgattg attccgccct gtgtttgtcc
      721 agggatatgg atcatataag ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt
      781 agaacttgtt tttccaacta attcagctag atttttattt cttgaattcg agacaccgta
      841 atggtcaccg taaaggacga tgactgaatt atcatacaag ccgctttctt tcaaataatt
      901 gaagaattct tgtacggcgg tatctaaata attggctgtc gcaaagtaac cgttgatcgt
      961 ttcatcattt gtattggcta acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg
     1021 ataatggtta gatacagcaa tgaattttga ataaaatggt tgttgcagac gttccaagta
     1081 ttgtacggat tgttggaaaa atggtttatc atgcaacccg tattggaacg aattgtcatc
     1141 attaacatca taataagaag aatcaaagaa ataatcatat cctaaattct tgtatgtctc
     1201 gtttcgattc cagaatgtac cagcgttccc atggaatacc gcactagtgt aaccttgtgt
     1261 ttgacttaaa atatcaggtg cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa
     1321 ggagccttga ttcaagccaa acaatgaatt ttccatcagc gtttccgcat cgcttgtttt
     1381 tcctgctttt acttgatgga aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag
     1441 actattgatg aaaggtgtaa cttcatgctg gagaccattc tcatctgtca atttataatc
     1501 gatcaggaat tgctggaagc tttctaaatg gatataaatc acattttttc ctttagcgag
     1561 cccgaacaaa tcgtcatttg gtgctgcata atgttctttt acataatttt ctacttcttt
     1621 catatcattg ggacttgctt ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc
     1681 ataaaccatg aaagcattca agccaagata tttgaccaaa taatcacgag agaatgttcg
     1741 agtcagcaat tctggacgat cagcttccgc cataaaaaga tttcctgaga aaatcataac
     1801 tgctaatgta gagatcgcaa aagccattct tgctcggatc gggcgagggt ccaacttgat
     1861 tttttttgca atcagcagaa cagtcaagat aataaaatcg atccaataga tcacatcata
     1921 tgggcgaaac agtcgaatgg cactttctcc cagaccactc gctactttcc cagcacctaa
     1981 aatcgtgttg atcgtaatga agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac
     2041 aagcaatgtc aaaagaaaat aaatgaccat catcgtgata taagcaactc tagggcgtct
     2101 gatataaaga gcaattccaa gtaataacaa tgtagttgca atagggttga taaacaagat
     2161 aaagtattga atgccacttt cgattccgag atggaaatca actaaatagg caaaaatcgt
     2221 tttcaaccaa agtaaaacga ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc
     2281 aggtgttttt atacatttca agataatctt cctttctgca cttcttaatc acagaagtgt
     2341 catgaaacca tgttacacag ctcacttatt ctcatttatt ttactagtat gacaatatag
     2401 tgtcaattga aaccagaagt ttatgctgtt tcattaaggt tttctttaag cttcatgaaa
     2461 aaatgagcag taagacaagt cctatgtttt ttgttatact tgggaagaga aagctaaagc
     2521 aggtgaagga atgaaagtaa aagtaaaaaa aagagcaagc aatcgattga aaaaaggata
     2581 tccactgatc caaaaagaag accttgctca tccccaaaaa gaaaccacgc aatgggtgga
     2641 attagttgat tcgcaaaacc aatttatcgc aaaaggatat ctaggaaagc agaataaggg
     2701 aatcggttgg gtattgagcc agcagaacat cccatttgat caatcttttt ttgaagaatt
     2761 ttttgtgaag tcaaaagaaa aaagaacttt ctttttttca gatgattcta ctaccgcttt
     2821 tcgtatattc aatggagaag gagacggaat cggcggtctg attgttgatt attatgcagg
     2881 ttatgctgtt ttttcttggt ataacgaaac gttatatgca catcgtgaat tattgattca
     2941 agcttttcgt gaaatctacc cggaaatcgt aggaggctat gagaaaattc gatttgaatc
     3001 agtaaatctg cctgagagcc agttcattta cggaaaagag gcaccggaac ccttgatcgt
     3061 aaaagaaaac ggagtaaaat ttgccgttta tttaaatgaa ggattaatga ctgggatctt
     3121 tttagaccaa aaagaagtac ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt
     3181 tttgaacatg tttagttata ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat
     3241 cgaaacaaca agtgtcgatc tagcaaaacg cagtttaaaa aaaacacagg aacagtttga
     3301 agtgaatggt ttggatgtcg atcagcaaaa aatcattgtt atggacgtat tcgagtattt
     3361 caaatacgcc aaaagaaaac aatttttata tgacatgatt gtattggatc caccaagttt
     3421 tgccagaaat aagaaaaaag tctttcgcgt agcaaaagat tatggggaac tggtcaagga
     3481 tagtctggat attttagcag aagacgggat acttattgcg tctacaaatg ctgcgaatgt
     3541 ctcgatagaa caattccaaa caatgattga agaggaattc catgccaaaa atgtttcttt
     3601 tgaacaactt gctttatatc gtctgccaaa agatttccag acgacggata cctttttaga
     3661 aggcaattat ctgaaagtgt ttgtttatca aattaaaaat aaccattaga aggagcgatg
     3721 cagaatggtt tgggtaaagg gattgaatat aggaagcggt agaccaaaga tttgtgcgcc
     3781 tcttatcggg gccgtccgag aggaagtatt acgtgaagca gttcttgcac gtgaagcagg
     3841 ggccgattta gtcgaatggc gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt
     3901 agcagaaaca ttggttctta tccatgagat gcttgatgga ctgccgttat tatttacgtt
     3961 tcgtacctta gctgaaggcg gagaacaaga catatcgatc cgtcagtatc gcgaccttta
     4021 tgaacatgtg atacacacag gattagtaga tatggtcgat atcgagttgt tcaaagtaga
     4081 aagtctagga aaaagactga tagaggaaat caagtcttta aacattcctt taattatcag
     4141 tagtcatgat tttaaagaaa caccagcaga ccctgtcttg ctttaccgat tgaatgttat
     4201 ggaacatttt ggagccgata tcggaaaact ggcagtaact cccaataatg aacgagatgt
     4261 tttgcggttg atggagttga ctagacgggc aaatgctttt gtctccatgc ctattattac
     4321 gatgtccatg ggaaatcttg gtaaaatttc tcgtctggct ggcggaacga cgggttccgt
     4381 tatgacattt ggttccttaa gcagcgattc tacttctgct ccaggacaat taccggtcaa
     4441 agcattgaag caaattattc aattattaaa tgaaccgttg tagaacaagt ttgaggtgat
     4501 attttttggc aaagaaaaaa atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa
     4561 agattcccta tacagagcac gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg
     4621 tcgctgaaca gcttccagaa agccaatctc gtatatttaa aacactggtt gctgtgggca
     4681 atgtgacggg gcctcttgtt gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat
     4741 tagcaaaagt ttcagggaat aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa
     4801 cgaccggata catacgtggt ggctgctcgc ctataggtat gaaaaaacta tttcctactt
     4861 atttagatca aattgcagaa agctatgaac aaatcattgt ctcggctggc agaagagggc
     4921 tacagatgga gttagcacct caagatatct gtgcattaac gagcggacaa tttgcagata
     4981 taaaacaata aatcatacgt ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc
     5041 tccttctttc ttttttatac attatgctct ttgcccggca ttcattcctg ctacatgccc
     5101 tgtagcgaaa gccgctgtga tattaaagcc gccagtataa ccattgacat ccagtaattc
     5161 tcccgcaaaa tacagaccat caatgatttt actctccaat gttttaggat aaatttcctt
     5221 tagagaaacc cctccgccag ttacaaatga tttctcaata ggaaaagttt tgttgattga
     5281 tagcttaaac tctttgcata gttgaacaaa atcttgaatt tgtttttgag aggcttgttg
     5341 tcctgtcaga tgatccattt caagccgctc aagaaagaaa actaacaatc gctcaggcaa
     5401 tataccatgc caagcattga ccaagttctt tttggttgct ttactttttt ctgtcaatat
     5461 atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc agagaaagtg tcactggttc
     5521 tcccgttttc tctaattctt tattgacaaa acttgaacaa cgcagtgcag ccggaccaga
     5581 aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca gtcaatacac gacctttttt
     5641 atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat
     5701 atacggttca tccgatatca agggggactc tgtcgcataa agaggcgtca ccgtgtgacc
     5761 cactcgcttc acgatcttgt acccatctcc agtagaacca gtagatggat aagtccgtcc
     5821 accagttgtt aagataatgg caggagcatg gaactcttcg aattccgtgc gtataccgta
     5881 aattttatgt tctttgtgga tcaatttctc tactcgagta gagaatagaa gcgtcacatc
     5941 tagttctttc agacgattga gcaatgcttc gataatcgtt tttgatttat ttgttacagg
     6001 aaacatccgt ccatgatcct cttcttttaa atgtacgcct tgtgattcaa agaagttcat
     6061 gatatcgaaa ttattccatt gcgcaaacgt actatataaa aactttccat ttccaggaat
     6121 atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca ttgcaccgac caccacctgt
     6181 catcagtagt ttttttcctg cttttttgtt tttttcgatc aagagtacgc gtgcgccttg
     6241 ttcagctgca ctgatcgctg ccatcatccc gcttgttcca gcgccgacta cgatcacatc
     6301 atacagtttt tccattccct cacctcaagg aaagtgtagc atatctgaag aaagaaaaca
     6361 gaaaaaaaat gttttgagcc aaaagagaaa atgagctaaa atgagggaag ataagcaaag
     6421 gagcgtggaa cgatgacaaa cgcaacagaa tatgttcaag caattcaaga aaagctacat
     6481 caaaaggatc aagggcaaga agaattttta caagcaatcg atgaattcat gcccacggtt
     6541 atgtcttttt tggacactca tcccgaatat atcgaaaaga atatcttaga acttttaacg
     6601 gaaccagaaa gagttattca atttcgagtg ccttggcaag atgatgaagg aaactggcga
     6661 gtaaatagag ggtaccgtat ccaatacaac tcagctatcg gcccttataa aggtggattg
     6721 cgcttccatc ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga acagattttc
     6781 aagaatagtt taaccggtct gccaatcggt ggaggaaaag gcggaagtga cttcgatcct
     6841 aaaggaaaat ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca
     6901 aaacatatcg gcccatcgat tgatgttcct gcgggagata tcggtgtagg agctagagaa
     6961 atcggctatt tgttcggcga atataaacga ttgaaaaaat atgatactgg tgtgttgaca
     7021 ggtaaacctt tggatttttg gggaagtaag atacgtacgg aagctactgg ttatggatta
     7081 gtgtattatg tcaaacatct attgaacgaa gaaaaagatt catttaatca aaaaacagtt
     7141 tttgtttccg gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca agagctaggc
     7201 gggaaagttg ttacatgttc agattctagc gggtacatct atgatccaga aggaatagat
     7261 gtgaaactat tgaaagaagt caaagaaatc aatagacaac gtttgacgga atacgcaaac
     7321 caacgtcctt ctgctattta tcatgctgga gaatctgttt ggacattgaa agaaaaagcg
     7381 gatatcgctt tgccatgtgc tactcaaaat gaaatagacg aaagcttggc cgaaatctta
     7441 gtagaaaatg ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac agtaaaagct
     7501 gtttctgttc ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc taatgctgga
     7561 ggagtagcag tatctgcttt agaaatgagt cagaacgtcc agcgattgac ttgggaaaga
     7621 gagcaagtgg atgagcagct ggatcaaatc atggaaacga tttaccatac gtgtcgccaa
     7681 acagcagccg agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt
     7741 gaaaaagttg ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg
     7801 aaatattgtc agcattttgt ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt
     7861 acctgtagag taaacctata aggaggagaa caaatggcac acattcattt tgactattca
     7921 aaagtagcac cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca agtaacagca
     7981 gcccacaacg aattacggga aggtactggc gcaggaagcg acttccgcgg ctggatcgat
     8041 ttaccaacga actatgacaa agaagaattt gcacgtatca aagaagcagc taaaaaaatc
     8101 caatctgatt ctgaagtatt agttgttatc ggtatcggtg gttcttattt aggcgctcgt
     8161 gcagcgatcg acttcttgaa tcacacattc ttcaatttat tagatagcaa cacaagaaaa
     8221 gcaccacaaa tcttctttgc cggaaattca atcagctcaa catatattgc tgacttgatc
     8281 gaagtaatcg gtgatcgcga cttttcagta aacgttattt caaaatctgg aacaactact
     8341 gaaccagcga ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata tggacaagaa
     8401 gaagcaaaca aacgtatcta cgctacaaca gataaagcaa aaggtgcagt taaagtagaa
     8461 gcagatgcag aaggttggga aactttcgtg attcctgatg acatcggcgg acgtttctct
     8521 gtattaactg ctgttggttt gttaccaatc gctgttagtg gtgcagatat cgatgcattg
     8581 atgcaaggtg ccgctgatgc aagcaaagca tactcaagcg acaaactaga agaaaacgaa
     8641 gcttaccaat acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt aactgagttg
     8701 ttgatcaact atgaaccagg aatgcaatac ttctcagaat ggtggaaaca attgtatggt
     8761 gaatctgaag gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat
     8821 ttgcactctt taggacaatt catccaagaa ggacgtcgca atatttttga aacagttgtg
     8881 aaggttgaaa aacctcgtaa atcaatcaca attcctgaac aagcagaaga tttagacggt
     8941 ctaggctact tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt tgaaggaaca
     9001 ttgcttgctc atacagacgg tgacgtgcct aacttgctag tcaaaattcc agaaatggat
     9061 gcttatacat tgggttatac aatgtacttc tttgaaattg ctgtaggtat ttcaggttac
     9121 ttgaatggaa tcaacccatt cgaccaacca ggtgtagaag cttacaagaa aaacatgttc
     9181 gcacttctag gcaaaccagg ctttgaagat cttgctaaag aattgaacga acgcctgtaa
     9241 gataagggtt tttcgctaga tttaaataat cagtgataga aacatgttcg ctttaaaatg
     9301 aaaactggta cccatttagg tactcaaaca tactgcatcg tttattcaga ataaggggct
     9361 gtgacaagac gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt gccttgctct
     9421 ttagtttgtt ttaggacttt aagaacagtg aggctgactt cgaaggttaa ttctgttttt
     9481 ttgttttcta gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt tgcgatatat
     9541 tcttagctta agataggcct aatttttctt ttacagatga gggtacctta tcgctggtaa
     9601 ttactttttt ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg
     9661 ggtaagcaga accattggca gagtcaccag aagtaatgac atcgaaatct aactgtcttt
     9721 tttcaccgtt ttcagcataa gcatctacgt tgtatttaaa acctcttcca attacttccc
     9781 cattatctgc tttgatatca gtttctgcag gcattggagc tttgactacc gcataataat
     9841 ctttacctac ataagtagac tgatagtagt cccaaccttt ccatcctata aaagcaatca
     9901 atatgataac gatcaaccct aaaatttttt tcatgaaaga tctccttttt tatttttatt
     9961 a
//
LOCUS       DAZAOG010000097         9955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000097 DAZAOG010000000
VERSION     DAZAOG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.097"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..3365
                     /gene="fss3"
                     /locus_tag="ACHDLD_001965"
     CDS             138..3365
                     /gene="fss3"
                     /locus_tag="ACHDLD_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN3363652.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3478..3792
                     /locus_tag="ACHDLD_001966"
     CDS             3478..3792
                     /locus_tag="ACHDLD_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3363653.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3805..4179
                     /locus_tag="ACHDLD_001967"
     CDS             3805..4179
                     /locus_tag="ACHDLD_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3363654.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4189..4524
                     /locus_tag="ACHDLD_001968"
     CDS             4189..4524
                     /locus_tag="ACHDLD_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN3363655.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4607..5947
                     /locus_tag="ACHDLD_001969"
     CDS             4607..5947
                     /locus_tag="ACHDLD_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN3363656.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6025..6699
                     /locus_tag="ACHDLD_001970"
     CDS             6025..6699
                     /locus_tag="ACHDLD_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3363657.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6692..6856
                     /locus_tag="ACHDLD_001971"
     CDS             6692..6856
                     /locus_tag="ACHDLD_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363658.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6932..8074
                     /gene="dcm"
                     /locus_tag="ACHDLD_001972"
                     /pseudo
     CDS             6932..8074
                     /gene="dcm"
                     /locus_tag="ACHDLD_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8064..8354
                     /locus_tag="ACHDLD_001973"
     CDS             8064..8354
                     /locus_tag="ACHDLD_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363659.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8613..9797
                     /gene="mobT"
                     /locus_tag="ACHDLD_001974"
     CDS             8613..9797
                     /gene="mobT"
                     /locus_tag="ACHDLD_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN3363660.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9794..9931
                     /locus_tag="ACHDLD_001975"
     CDS             9794..9931
                     /locus_tag="ACHDLD_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN3363661.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3296 a         1679 c         2248 g         2732 t
ORIGIN      
        1 ttagattaaa tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt
       61 ttaaaaagac tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt
      121 tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt
      181 tgagtttggc tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg
      241 aaattaccca tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg
      301 aaggaacttt tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt
      361 acaacggctt taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt
      421 tcactcctgg ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt
      481 ccgttttatg gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga
      541 tctggcaaga agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg
      601 taaatatcgc agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac
      661 caaccttcca taatagtacg gctaaaacag tattagggca atcgactact gtgacggata
      721 cgaataatct gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt
      781 atcgtgtgaa tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg
      841 tgctgacgct taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc
      901 aacagacttt aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg
      961 tggaaacgga gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac
     1021 caggaacggt tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg
     1081 acaaagaagg cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg
     1141 aaaaatcagt accggcgcct tatacgattg ataccacccc catgactacc accattaaag
     1201 caggcgaaac catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg
     1261 ataaaagcgg agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta
     1321 atacgtttgc cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg
     1381 atgaaaacgg tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct
     1441 attatgtgac cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag
     1501 tcgaattgaa gtatgccaat caaaccgtag cccctgttac cagtaacgta aaagggcaaa
     1561 atcaagaagt cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag
     1621 cacaaggtaa ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa
     1681 aagctgttaa atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg
     1741 atgaaacggt cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta
     1801 tcaacgagta ttattggcaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa
     1861 agtatcctgt atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa
     1921 atgttacggc aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat
     1981 cagctgcggg cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg
     2041 aagggaccaa tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat
     2101 tagggtttga tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag
     2161 aagtagaagc cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta
     2221 aggaaaacaa agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc
     2281 aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt
     2341 ctgttagttt gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt
     2401 ctcttgcgac ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag
     2461 ttgataaatt gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag
     2521 ccattgatgt ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg
     2581 tgattgcaga aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc
     2641 agcataaatt aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt
     2701 atgaaaataa ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga
     2761 ataaccaagc ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg
     2821 cccacctaga aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg
     2881 atgtgtcgat tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt
     2941 atgctttatt accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg
     3001 aagtaaatga taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat
     3061 atccagaagg aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca
     3121 atggaaaaca caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc
     3181 caaccatact aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag
     3241 aatctagtcc cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac
     3301 tgttcattgg ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga
     3361 actaaggtga tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta
     3421 aaaaagtagt cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg
     3481 gaattgaaat ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt
     3541 gaaggagaaa ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat
     3601 catttgtatt ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc
     3661 ggtgaaaagg attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc
     3721 aaaggctatg agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta
     3781 gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt
     3841 ttaacatttg ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag
     3901 gatggaacaa ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa
     3961 gggcgaatga ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat
     4021 gcagaggtag agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa
     4081 ggaactaccg ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat
     4141 caaggaaatc ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat
     4201 gttgaacata ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa
     4261 ctcgttaaag cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt
     4321 ggagaaaata gcgacagtga agaagactct gatgtactag actggttagg agaagaagag
     4381 tctttcttta tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg
     4441 gaagaaattg gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa
     4501 acgaaacaac tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta
     4561 atgaaatcct attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca
     4621 tcgcatacgc gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc
     4681 tctgttcatc ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc
     4741 taagctatca ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct
     4801 tggaagtctc gctttttcag gagctatgat attgctttat attcactttt ttcttgatca
     4861 ttggcgaagc ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta
     4921 tgaagtgaaa caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag
     4981 agagactatc agttacttcc ccaaaatctg ttatcgaatg aaagatggat tactgtctat
     5041 tcgcgttcaa atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt
     5101 agaaagcgga ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta
     5161 tactttactg tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa
     5221 tgggactctg cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt
     5281 aatcgctggt ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact
     5341 attgaagtca gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt
     5401 aggtacggtg atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga
     5461 tttctatgaa cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac
     5521 aggagagaat tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt
     5581 ggcttatatg gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca
     5641 aatcgtgatg ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga
     5701 tgcgaaatat ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg
     5761 tatgagtgaa ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa
     5821 gcacatcaaa ggtcgaggtt atgtggatac aggtggcagt gtgattagtg agttttatac
     5881 gccacttgta ccaaaagggt atgatttctt agattcaatt agtaaattag aaaagatgaa
     5941 tgattgagtg atcttcaata ctgactttat acttcaagta acgtataata cttttatata
     6001 gtaggaagaa aggggataat taatatggaa agtaatgatt cttatttaga gtatgcaaaa
     6061 gcaaataagg ataagtttat ttcttatatt atcaaaggga aatcccccga agttggacga
     6121 gacgcagttt ttatggcagg gagtcctggg gctggaaaat cagaggttgc cattggatta
     6181 gctgagaatt atgataacca tgtgattatt gatgcggatt actttcgcac ccagtttcct
     6241 gattataatg gaaaaaactc tagtctcttt caaaaagcat cttcttggtt ggtagagcag
     6301 gcatttagat atgtcttaga tcatggctat tcttttattc tggatgcaac ttttgcaata
     6361 ttaagtgctg aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc
     6421 tatgtttatc aagaccctaa aatcgcttgg aaatttacac aagaacgtga aattgcagag
     6481 ggacgtcatg tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag agagaacatt
     6541 gcaaaagtaa agagtaaaca tccagatgta ttgcttcata tcataataaa agattatacg
     6601 aataatattt ccgaagttca ttatgctacg gataatctag aattattttt accactaggg
     6661 tatagtagga aagatttgga ggttcaatta catgactgag aaagaacaag taacgaaaat
     6721 tgtaaagaaa tacaataaga gcattgcgga tttatcggaa aatgctactg caaaagagtt
     6781 caaaacggtg attaaatatg tagccgatca ggccaatgaa aaacaacgga aattagttgg
     6841 attaaataaa aaatagtagg acaaattaat aatgtatgag ccaagagcaa aatgttcttg
     6901 gcttttttct ttgcaatgaa tgaggtaatg tatgagattt ttagatttat tcgccggtat
     6961 tggtggcttt cgtttaggta tggaacaggc gagccatcat tgtatcggtt tttgtgaaat
     7021 tgataaattt gctagacgta gttataaagc aattcatgat acaagtaagg aggtggaaat
     7081 gcatgacatc acaagtgtat cagatgagtt tattcaatcc ctcggaccag tggacatcct
     7141 ttgcggcgga tttccgtgcc aagctttttc aattgcggga aagcggcaaa gattttccga
     7201 tactcgaggt actttattct ttgaaatcgc tcggttcacc actcttctcc gacctaagct
     7261 tttattcctt gagaacgtcc gaggattgct caatcacgaa ggaggggcta cgttcgagac
     7321 gatcctccga acgctggatg gattggggta tgacgtggag tggtaggtgc ttaactcaaa
     7381 ggcctacatt ccccaaagcc gggagcggat attccttatc ggacattctc gagacgcatg
     7441 taccgaacaa gtatttccta tcattggacc gtcttcaaca tctgatcaaa acatcagaaa
     7501 tttggtaaat atcaatcctt ccaatcgagg aatgggtgga caagtctatg gttcagatgg
     7561 cgtagcgcca accttaacag gtgatgaagg aatcaaaatc gcactgccag caagggatgg
     7621 aatctctgta gcaggactat taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt
     7681 tactggaacc gcccccacct tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa
     7741 aaatgaaaca gaatatcaag aggtgaagcc agaagacagt gtgaatctgg cttttcctaa
     7801 ttccacaagt cgccgaggtc gtctgggaaa acagagtgtt cataccttac taacaggaga
     7861 ccaacaagcg atcgtcacgg atcagtatca aattcggaag ctaacaccta gagagtgttg
     7921 gcgacttcag ggctttcccg attgggcttt tgatcgagat tctcaagtca attcagatag
     7981 tcagttgtat aagcaagccg gaaactctgt aacagttccg gtcatttttg atattgccag
     8041 aagattaaaa gagttggatg tacatggttt atgatgagtt gattggagaa atttattggg
     8101 tgattggaaa gattcagtca gacccagaat tagaagaaca attgcgacgg ttaaaatttg
     8161 atattcgcaa gaatggtgtc aaagtcgctg gcgatccata tttaatgaat gaggagacgg
     8221 atgctcgaat tgaaataaat caagtaattg cagaatttga gcggattgcg gaccaagcaa
     8281 aagaaccaga tattcgtcaa tacttgttag aaatgaaagc agagttggaa gtaaatggta
     8341 ttacagatga gtaacctaaa ccaacacaac tgactgttag gcgctgaccg cggaggattc
     8401 gcgaagcaga aatcctccgt ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga
     8461 tgtctcaccc ccgtatctaa caggggggta caaaaacaca aaaaataagt aagcaaccat
     8521 taagaatgct tgttgtatca aagatagtaa gtaatttgta taggtgttaa ctcatttgaa
     8581 aaagttaaca ccttgaattt ggaggtgaga acttggcaca acggaattta gattatcggc
     8641 tattaaaaga ccgacgcaac gaatacggcg tttcacaaaa taaattggct acggcttgtg
     8701 gacttagtcg gccgtactta aatcagatag aaaatggtgg cgtaaccgca tctataaaaa
     8761 ccatgaggaa aatttttgac caactggaaa gctttaatcc cgatttgcct ttgacgttac
     8821 tgtttgacta tgtaaggatt cgctttccca caacggatgc acgaaaaatt attcaagaaa
     8881 ttctccattt gaaatttgag tatatgctcc atgaagatta tgccttttac tcctatcaag
     8941 aacagtatgt catgggtgat attgtggtga tgttgtctca tgaagaagat aaaggtgtgc
     9001 ttttagaatt aaaggggcga ggttgccgac aatttgaaac ttttttactc gctcaaaagc
     9061 gtagctggta cgactttttc gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg
     9121 acttggcaat caatgatcga gtggggctat tggatatacc tgagctaacg aaaaaatgtc
     9181 agaaggaaga atgtatctcc ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt
     9241 taaaggctga tgaaaaggac ggcatgggca ataccttata tatcggcagt ctcaagagtg
     9301 aggtttattt ctgtttgtat gaaaaagact atgaacaata tatcaagtta gggattccat
     9361 tagaccaaac tgagacgaaa aatcgttttg agattcgact gaaaaatgac cgtgcttttc
     9421 atgcgattca agatttgtta aaaggtcgta gcattgagag caccacgttt tccattatca
     9481 atcgttactt gcgatttgcg gataaagtag aaggtaaaag gcgaaccaac tggcccttga
     9541 atgaacaatg ggggcgtttt attggacgga atcggaaaga gattcaatta acttccgagc
     9601 caaaaccgta taccattgaa cgaaccttga attggctagg ccgacaagtc gcccctacgt
     9661 ggaaaatggc taaggagttg gatcgattga atcggacaac ctatatccag gatatggtac
     9721 agaatgcacg cttatcagac cgacataaaa agattttgga gcaacaaagt atggcaattg
     9781 aaaatttgat tatatgaaaa gaggaatcgt catgggaatt gtaaaagata tttttttggt
     9841 atttggtggc agtatgcttg gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc
     9901 ggatcgagca atggaaaaga aaggaaagtg acaagatgaa tttcggacaa aattt
//
LOCUS       DAZAOG010000098         9945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000098 DAZAOG010000000
VERSION     DAZAOG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.098"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..231)
                     /locus_tag="ACHDLD_001976"
     CDS             complement(34..231)
                     /locus_tag="ACHDLD_001976"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN3363662.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(377..1255)
                     /locus_tag="ACHDLD_001977"
     CDS             complement(377..1255)
                     /locus_tag="ACHDLD_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3363663.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1294..2412)
                     /locus_tag="ACHDLD_001978"
     CDS             complement(1294..2412)
                     /locus_tag="ACHDLD_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN3363664.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2409..4508)
                     /locus_tag="ACHDLD_001979"
     CDS             complement(2409..4508)
                     /locus_tag="ACHDLD_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN3363665.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4559..5380)
                     /locus_tag="ACHDLD_001980"
     CDS             complement(4559..5380)
                     /locus_tag="ACHDLD_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3363666.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5377..6186)
                     /locus_tag="ACHDLD_001981"
     CDS             complement(5377..6186)
                     /locus_tag="ACHDLD_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3363667.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6207..6701)
                     /locus_tag="ACHDLD_001982"
     CDS             complement(6207..6701)
                     /locus_tag="ACHDLD_001982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3363668.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6713..7213)
                     /locus_tag="ACHDLD_001983"
     CDS             complement(6713..7213)
                     /locus_tag="ACHDLD_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3363669.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7278..>9945)
                     /locus_tag="ACHDLD_001984"
     CDS             complement(7278..>9945)
                     /locus_tag="ACHDLD_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3363670.1"
                     /translation="QPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTGNMSSVTAEKI
                     SKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVG
                     HDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEKDAPF
                     AILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEVHRLSKEN
                     QEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQISVMLPTF
                     EDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKNIIKISCA
                     DVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSI
                     LEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGK
                     TYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSE
                     KDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMP
                     VESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTS
                     IALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKD
                     VFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYD
                     ILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETV
                     ISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSR
                     SIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3018 a         2248 c         1647 g         3032 t
ORIGIN      
        1 ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc
       61 aaggattatg aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat
      121 taccacagac ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat
      181 cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca
      241 gtgatttaat gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc
      301 tttatccaac ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca
      361 atgaaacatt cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc
      421 cgccgattat tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg
      481 gtatttcaac atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt
      541 gttcttgttt cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg
      601 tattgtatgc acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca
      661 ataaattctg gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct
      721 ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga
      781 tcaacatatg ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg
      841 caccacctcc gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag
      901 aaccagcaac atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa
      961 tattgaaata ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg
     1021 ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc
     1081 cgaccccgat gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca
     1141 ttgtttcaac tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag
     1201 taccgactgc acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag
     1261 gttcctccct tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt
     1321 gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac
     1381 atcgtcttgc cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga
     1441 taaatcttcg gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg
     1501 cattgcgtcg tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct
     1561 tgtggcatct ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc
     1621 atgtggacgt ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc
     1681 tgaacctgtt gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc
     1741 gccttcgccc caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata
     1801 gccttgaccc tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc
     1861 ccgatgccaa tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta
     1921 atttatcgta taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt
     1981 agcggatgtt tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac
     2041 tatcggcaac gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa
     2101 aggactttca acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg
     2161 taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat
     2221 tcccgttcca gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc
     2281 acgacctttg cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc
     2341 tgttggattt tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt
     2401 tagtatactc atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga
     2461 atttttttcg tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg
     2521 gattgcgtac attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac
     2581 gttaaattat caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat
     2641 tgactgatca ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag
     2701 tctcgcacga cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg
     2761 tctaaatctg ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc
     2821 agctcgctca ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa
     2881 gtgatgcctt tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg
     2941 gtgttctgat ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg
     3001 tctgattctt ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt
     3061 aaaaagatta acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt
     3121 ttctcctcat caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt
     3181 tgtttacaaa ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc
     3241 agttgttcta gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga
     3301 ttgctgccgg tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa
     3361 agcattggtt cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc
     3421 agtagaacct gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga
     3481 ctagccagta acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat
     3541 tttgaaccag gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac
     3601 tccggttcaa acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga
     3661 ttagtagcag gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg
     3721 aaactagaaa agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc
     3781 attgacagct gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg
     3841 atatcagatt cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg
     3901 gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac
     3961 acattttgct gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga
     4021 aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta
     4081 ttagccagta tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata
     4141 tatctaggca aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa
     4201 tggtagacaa tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt
     4261 ttattgaccc aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac
     4321 gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag
     4381 gtcaatccct ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca
     4441 tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta
     4501 atagtcatct tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact
     4561 atgcgattcc aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga
     4621 tgacgccgac ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa
     4681 ttcctttaaa aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg
     4741 aaaagctcaa aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca
     4801 gacccaagat actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt
     4861 caatatattt agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat
     4921 taggtatatt gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta
     4981 agttgatcgc tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa
     5041 ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac
     5101 cagctttttc agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc
     5161 aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt
     5221 cacttttgta gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca
     5281 tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca
     5341 attttttctc atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc
     5401 ttggtcaagc gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt
     5461 tagttgtaga tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc
     5521 aatcaatggt aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat
     5581 atttttactc caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc
     5641 agcgactgtt aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac
     5701 atctgctcca aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa
     5761 ccacataaag aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa
     5821 aaatttagca gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc
     5881 taatactgct agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat
     5941 tgcgaacgct gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat
     6001 attgacgaca cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa
     6061 atcccccatc aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat
     6121 cccgaacatt tcatgtccag caattccaat aaaaacggcc aaagcgacta caaatgcatc
     6181 atacataatt tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt
     6241 ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt
     6301 aagtcttgaa tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc
     6361 gatggcggtg tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa
     6421 gctttcgcaa cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc
     6481 ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg
     6541 acattcgatg gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca
     6601 tcgcttgcta ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt
     6661 ccatgaatta atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa
     6721 aatcttcctc ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt
     6781 ggcatttatt taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta
     6841 tttcgagcag aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca
     6901 aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa
     6961 tctgatcctg ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact
     7021 catccacata agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac
     7081 tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt
     7141 taattccttt ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca
     7201 ttggtttaat cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag
     7261 acactttttt agacacttta tttttgaata tacggtccta ataattcata gagcagcgaa
     7321 agctcataag cattgacttt aaaattatat ttcatctcca ttgattgaaa aatacttcta
     7381 gaaacataat aaaattcatc ttcatctggt ttcaaatcat aattcggttc ttcagatttt
     7441 tctgtccgag ccaagaataa acgttccatc atcaatgccg tatgcatata gagatttagt
     7501 tttacacggc catttaaagt gaaatgatag taattttcat atttagaaat gactgtttcc
     7561 acttcactca tcacgatcgt tgggttcaga aagttcaaac ggtccgcaat tccttccaac
     7621 gaaaagaatt ttactagctc ctgaatcaag cggttaaatt ctgccggctt catttcagga
     7681 ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta agatgtcata aacattaata
     7741 ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca
     7801 aaataagttt catcattttg gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa
     7861 acttcgatgg attcatccga taaatgatga cgaaaaacat ctttcaattt gtccgaaatc
     7921 cccaaacccg acatacagga aatgataatg ttgctacttt gagaaaagcc ttcaaagtat
     7981 tgaacattga ttgaatactt gtctgctgca tcctccgcaa tctgtttaaa tggcgcattc
     8041 atcgtcatct tcaagccaat atcaagtgca atggaagtcg tcaaattatt cagaagaagt
     8101 aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt gatttaaaga gcccatatct
     8161 acgatcagca ctagactatc tgtattcact tgccgtttca cgaaattcct tgtcgattcg
     8221 acaatctctg acacattact ctctactggc atatccaacg cttcaaaaac ataattgccg
     8281 cataattgat tcactactgc ttgaatgctg ctagccgtac tatctccgtg agcaagcaac
     8341 aggcccttca gctcgatgga ttcatctaca taatcagata aagtcacgac aataagcagt
     8401 cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat gctccaagaa ctgattacat
     8461 aagtaagtcg tccgaggtag atttgcagct aatgtacgat ttatcccggt taagtctgtc
     8521 ttatagcttg gggaaatgac ataggatctg ctaataattt ctgcaacagg tccactcgtc
     8581 gtcaatccgt acgtttttcc taaaacggat acccagacct ttctaaacat ctgaatcatc
     8641 aattttctac tcattgataa aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact
     8701 tcattcttta tcgaagaaaa attttcattg aaattacctt catttatcag ctgagtaatt
     8761 ttttccaaaa tcgaatcata atcaaacggt ttgattcgat ccgtttttat cttttgattt
     8821 ttctttatca ataaactagc aatcggcaag ctttctactt cggattccag catatttaac
     8881 tgaatcaaga tctcattttt cccaatcgtt aatggttctt cttgtgttcg actatacaca
     8941 tccgcacaac tgattttaat gatattcttc agcttaccaa tattcccact aaagggatga
     9001 gataacatta aagacaaggc ttctcttgaa acaataatat ctttttgtaa aacatccgct
     9061 tcattttgaa aaaataattg aatcagctct aatcgttctc gaatggggcg atcttcaaaa
     9121 gttggcaaca taacactaat ttgaatacgc cgatcaaagg tgtctaataa ataattttca
     9181 cctttttccg tcgtggcgaa aataaaacga accttagcag aatgccaatt tacattttca
     9241 ccgactggac gatagtttcc agtatcaata aatagaaaga gcttttcttg attttctttc
     9301 gataggcgat ggacctcatc gagaaaaaga tagccgttgt tggccgttgc caataaacca
     9361 tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa
     9421 agcagttcgg gattgtccgc ataatcggca cagtttaaga tagcaaatgg tgcatctttt
     9481 tcaataattt gctcatggat agcgtattca aaaatttttg tggctaaaaa actttttcca
     9541 acacccgtgg ctccatttat taatacactg agtccgttag gcggatattt gacagcggct
     9601 atacattttt gaatgacctc tctcagactt ccgtcatgac caactacaga atgaaaggct
     9661 tcgtttattt gctccgtatt atctacaacc gcatattcat ctaaaggctt ccacaaaaca
     9721 ggctttcccg gtaatttctc gaccttttta tcttccacta atctcgtcaa ataatggctg
     9781 gcattctgac gagagatacc tagattcttc gaaattttct cggcagtgac agaagacata
     9841 ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt tagcgctctt tttcattttt
     9901 tcctccagtt taatgtgtct aaaagcataa taaactatcg gctgc
//
LOCUS       DAZAOG010000099         9934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000099 DAZAOG010000000
VERSION     DAZAOG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.099"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(273..1256)
                     /locus_tag="ACHDLD_001985"
     CDS             complement(273..1256)
                     /locus_tag="ACHDLD_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN3363671.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1261..1908)
                     /locus_tag="ACHDLD_001986"
     CDS             complement(1261..1908)
                     /locus_tag="ACHDLD_001986"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3363672.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1993..2625)
                     /locus_tag="ACHDLD_001987"
     CDS             complement(1993..2625)
                     /locus_tag="ACHDLD_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3363673.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2641..3192)
                     /gene="coaC"
                     /locus_tag="ACHDLD_001988"
     CDS             complement(2641..3192)
                     /gene="coaC"
                     /locus_tag="ACHDLD_001988"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN3363674.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3189..3926)
                     /locus_tag="ACHDLD_001989"
     CDS             complement(3189..3926)
                     /locus_tag="ACHDLD_001989"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN3363675.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3923..4912)
                     /locus_tag="ACHDLD_001990"
     CDS             complement(3923..4912)
                     /locus_tag="ACHDLD_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3363676.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5170..5712
                     /locus_tag="ACHDLD_001991"
     CDS             5170..5712
                     /locus_tag="ACHDLD_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN3363677.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5729..6814
                     /locus_tag="ACHDLD_001992"
     CDS             5729..6814
                     /locus_tag="ACHDLD_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363678.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6826..7632
                     /locus_tag="ACHDLD_001993"
     CDS             6826..7632
                     /locus_tag="ACHDLD_001993"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363679.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7633..8460
                     /locus_tag="ACHDLD_001994"
     CDS             7633..8460
                     /locus_tag="ACHDLD_001994"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363680.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8457..9530
                     /locus_tag="ACHDLD_001995"
     CDS             8457..9530
                     /locus_tag="ACHDLD_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3363681.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3016 a         1823 c         2020 g         3075 t
ORIGIN      
        1 gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
       61 tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
      121 gctctaaaca cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt
      181 cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt
      241 ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt
      301 ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat
      361 gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata
      421 aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc
      481 agttggacac gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct
      541 agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag
      601 taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt
      661 acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta
      721 caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt
      781 ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt
      841 tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct
      901 gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca
      961 ggcatcacag catattcggc ataaccacct agattgacta atccgtatac ttttgtccca
     1021 ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc
     1081 aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg
     1141 acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact
     1201 tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat
     1261 ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag
     1321 tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac
     1381 tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt
     1441 aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg
     1501 tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca
     1561 ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt
     1621 ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc
     1681 ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc
     1741 cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg
     1801 ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat
     1861 caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct
     1921 ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg
     1981 ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca
     2041 tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac
     2101 cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg
     2161 ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag
     2221 tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt
     2281 ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc
     2341 tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa
     2401 aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa
     2461 agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt
     2521 tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat
     2581 agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt
     2641 ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc
     2701 taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg
     2761 atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt
     2821 cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt
     2881 tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg
     2941 cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt
     3001 tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg
     3061 cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac
     3121 gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa
     3181 gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca
     3241 gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc
     3301 tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga
     3361 ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca
     3421 atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc
     3481 aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt
     3541 tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt
     3601 gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg
     3661 ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt
     3721 gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc
     3781 gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc
     3841 cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt
     3901 cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt
     3961 ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt
     4021 ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt
     4081 gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag
     4141 gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt
     4201 tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat
     4261 agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta
     4321 cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg
     4381 caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc
     4441 cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt
     4501 tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca
     4561 tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc
     4621 ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac
     4681 gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg
     4741 ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat
     4801 aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg
     4861 ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga
     4921 cactcctttt aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt
     4981 ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc
     5041 aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat
     5101 ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg
     5161 gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca
     5221 caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga
     5281 gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact
     5341 ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat
     5401 catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg
     5461 aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg
     5521 tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc
     5581 ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa
     5641 caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa
     5701 tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt
     5761 agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg
     5821 aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat
     5881 agctggattc accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga
     5941 tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca
     6001 tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga
     6061 tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg
     6121 ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt
     6181 aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg
     6241 tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt
     6301 tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa
     6361 agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt
     6421 cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct
     6481 agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc
     6541 agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact
     6601 agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga
     6661 tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt
     6721 aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga
     6781 attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc
     6841 aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta
     6901 atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta
     6961 tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt
     7021 ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg
     7081 aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt
     7141 aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat
     7201 tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca
     7261 acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga agagatgaat
     7321 ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga
     7381 gtgatttttc cgttattatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct
     7441 tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga
     7501 acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt
     7561 gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga
     7621 ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta
     7681 ttcttatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg
     7741 aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg
     7801 atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc
     7861 atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg
     7921 ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc
     7981 ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat
     8041 attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac
     8101 gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat
     8161 atcattttgc cttatctttc tccaggaatc atagctggat attttatggc atttacctat
     8221 tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca
     8281 gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg
     8341 ttagttttct tgttctccat gatattggtc attggatact actttattag taaagaaaac
     8401 cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga
     8461 aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg
     8521 ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact
     8581 ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag
     8641 tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga
     8701 caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac
     8761 tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt
     8821 tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag
     8881 ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt
     8941 ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc
     9001 tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag ttgacagaag
     9061 cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca
     9121 aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg
     9181 atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt
     9241 ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca
     9301 tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta
     9361 caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct
     9421 atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc
     9481 taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact
     9541 cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg
     9601 agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt
     9661 tttctaccac gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga
     9721 gacgggctag taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat
     9781 gcaaatatac ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa
     9841 tatttgtagc tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa
     9901 attattttgt cagatatctg aggcaaatat tcga
//
LOCUS       DAZAOG010000100         9881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000100 DAZAOG010000000
VERSION     DAZAOG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.100"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(345..569)
                     /locus_tag="ACHDLD_001996"
     CDS             complement(345..569)
                     /locus_tag="ACHDLD_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363682.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            760..3537
                     /locus_tag="ACHDLD_001997"
     CDS             760..3537
                     /locus_tag="ACHDLD_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3363683.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3618..4037
                     /locus_tag="ACHDLD_001998"
     CDS             3618..4037
                     /locus_tag="ACHDLD_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3363684.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4049..4519
                     /locus_tag="ACHDLD_001999"
     CDS             4049..4519
                     /locus_tag="ACHDLD_001999"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3363685.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4536..5315
                     /locus_tag="ACHDLD_002000"
     CDS             4536..5315
                     /locus_tag="ACHDLD_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3363686.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5302..6123
                     /locus_tag="ACHDLD_002001"
     CDS             5302..6123
                     /locus_tag="ACHDLD_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3363687.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6140..7189
                     /locus_tag="ACHDLD_002002"
     CDS             6140..7189
                     /locus_tag="ACHDLD_002002"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3363688.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7202..8206
                     /locus_tag="ACHDLD_002003"
     CDS             7202..8206
                     /locus_tag="ACHDLD_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3363689.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8839..9858
                     /locus_tag="ACHDLD_002004"
     CDS             8839..9858
                     /locus_tag="ACHDLD_002004"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN3363690.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3314 a         1641 c         2066 g         2860 t
ORIGIN      
        1 aataatagtg gagaaaacta tggtttggcg cagattctat caggtttgat ggaacgaaga
       61 caacctcccg acatcaagtc aaaagctact tgagattttg atctgaaaat aaaaaaagaa
      121 aacaaggtag gaacatcaat gtttctacct tgttttcgta atcaatggga aaaaactaag
      181 agtggtgtac gtaatcaata aaaaggatgg tgacaacagt acctctttac tctctgaaag
      241 catcataact cgtttttcct cattatgcca ataaattgtt gttataccac atataaggat
      301 ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg aatcttattt tgtatggaag
      361 gatttataga acgcaaaaat caagataaag atgaataaaa atcctgtttc ggctatatat
      421 tgcgtatcta cgccaataaa acgagtttgt tctgtcatag ccatataaag caaaggcaaa
      481 aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt
      541 ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata
      601 tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata
      661 agggataagt aggctgctgt ctataataag atatattttt caattcttta ttaaagattt
      721 tattgacaaa atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag
      781 gaaattttat gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag
      841 gtctttacag cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat
      901 tatttgaacc agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt
      961 tatttccaca aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt
     1021 tctatcgatg atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct
     1081 tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt
     1141 tactatccag atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt
     1201 tatttagtta atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca
     1261 ccattcatca cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat
     1321 ttgtttggac atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt
     1381 gaagcagcag atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt
     1441 caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat
     1501 ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat
     1561 ttcttaacaa ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag
     1621 cgtagtcgta atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa
     1681 atggggagcc ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta
     1741 tccggaaaca taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct
     1801 gaacagcgtg aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt
     1861 gcgcagccta ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc
     1921 ttagagcaat taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag
     1981 caaatcctaa ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta
     2041 aagcaagaag tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa
     2101 cacgagctgc tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact
     2161 gcctatcaaa tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag
     2221 cgaagcacta tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta
     2281 gaacaacaag tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa
     2341 ttaagtaaga tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc
     2401 tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct
     2461 cacggttatg caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat
     2521 ctatttgaat catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc
     2581 cttgattatt gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga
     2641 tctctgaaag aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg
     2701 aataatgtga cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca
     2761 ttagaagaga tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat
     2821 ccagaggaaa acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg
     2881 gtccacttaa gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc
     2941 ccatatgaat atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac
     3001 gaagtcgtag gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca
     3061 ttagaagacc tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact
     3121 atgaatgctt cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa
     3181 aaagtactgg attccgtgac tattctagat acagagaagg tgatgggaga aatcgatcat
     3241 tttatgagag aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg
     3301 ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc
     3361 tatcaaggat tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct
     3421 gcgtttagtg tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat
     3481 atctatgata ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca
     3541 taagcaagtt gttgccacta attttggcat gcaacttgct tttttagtat gtgtaagtga
     3601 aaagaggaga tgaaaaagtg gatagagaaa ttatcttagc ttcccatgga aaatttgctt
     3661 cagggatatt agattcatta gagctgatct atgggaaaaa tcattctatt actgtattcg
     3721 attgctatac tgatgagaat tttgatttag cggaaacagt aaggcagctt tttatgaggt
     3781 acaaagataa agaaatcatt gttctgacgg atctttttgg cggaagtgtc aacaacgaat
     3841 tcttacaata tatcaatcgg gatcatgtct atctcgttgc cggattgaat ttgccactat
     3901 tgattgagct agtatctcgg ttggacgctg aagtagagac aactgttttg atccagcagg
     3961 tgctaactga ttcaaaaaaa atgatccagt tttgtaacga gcggctcaat cagaaaatag
     4021 aagaagacga attttaggag gaagaaaaat gattgtattg acaagagtcg accatcggtt
     4081 attacacgga caagtagctt tttcatggac acaaacaata ggcgcagact gtattttgat
     4141 tgctaatgat gatgtaccta cgaatgagat acgaaagaca acgatcaaat tagccaagcc
     4201 acaaggagtc aaactagtga tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg
     4261 ggtgacagat aaatataagt tattcatcgt agtggaatcg atcgaggatg catataagct
     4321 ggcagaagcg tatccagaaa tcaaaaccat caacttagga gggatcaagg caaaacctgg
     4381 tactcgcagc atcggaaaag cggtaaatat tttaccagaa gaggaggaac gcttgaaacg
     4441 attaattgac aaacaaatag aagtagaaat ccgtcaagta ccagcagata aaaaagtgga
     4501 tgtaagagaa gttttgtaag aaaggaagaa cctcaatgat tgtacaagca gtcttattag
     4561 gaattgttgc atttgtcgca caatcagaat atgcactagg gacatcgttg ctttctcgtc
     4621 cgattgtgac ggggctgttt acaggaatcg tgttaggtga tgtaaaaact gggatcatta
     4681 tgggcgcaac tttagagtta gcatttatcg gatcgttctc agtaggtgct tcgattccac
     4741 ctgatgttgt gaccggtggg attttaggga cggcttttgc tattacagct ggagccggta
     4801 cagaaaccgc gcttttacta ggtttgccga ttgctacgtt gacattgata ttgaaaaata
     4861 tttacttagg gcttttgatt ccaattatga accataaagc agacaattat gcagaagaag
     4921 gaaattataa aggagtcgag catatgcact tgcttgccgg tatcggtctt tccttgatgc
     4981 ttggtgttgt ggtaatggtt tcttatatgg tgggaagcaa tacgatcgga aatctattaa
     5041 atatgattcc tgacttcgtt caaaaggggt tgtcagtagc tacagggttg attccagctc
     5101 ttggttttgc gatgcttgct cgtttgctga tcaacaaaca agttgcacca tattttttct
     5161 taggttttgc tatagcttct tacttagaga ttcctgtgac cggagtagct atttttggtg
     5221 cgattttagc agttgtggta gtcaatatta tgaatgtccg tcaactacca cttcaaacaa
     5281 ctgcagggga ggtagaagaa gatgaagaat tctgagatca aaaacagcaa aataacgaaa
     5341 aaagagctaa atcacgtatt ttggcgttct tttcaaatgg agttttcttg gaattatgaa
     5401 cgtcagatga atatggctta tgtttatgca atgattccga ttttgaaaaa gttatatcag
     5461 tcgaaagaag aaatggcagc agctttgaaa cggcatctag agttttttaa cacgacccca
     5521 catatcgtta cactgatttt aggaatcacg gctgcaatgg aggaagaaaa taaggatgat
     5581 cctgaatttg atgtgactgc gatcgatagt atcaaaacat ctttgatggg acctcttgca
     5641 gggttaggag attctttctt ttggggaact ttgcgattga ttgccacagg tgtgggtacc
     5701 tctttagcca tgcaaggaaa tattttaggc cctatcttgt tcattttgat tttcaatatt
     5761 ccgcacattc tgtttcgcta tctagcaaca ggttggggat atcgcttagg aacgggtttt
     5821 ttgaaaaaga tccaggcaaa cggtatgatg gaaagtttga cattaggcgc gtctattatt
     5881 ggactgatgg tcgttggagc aatgactgcc acgatgatca ccatcaatat tcctttaaaa
     5941 atcggttcag gagaaaatgc tgtgactgtt caaagtattt ttgatgatat cgttccaaat
     6001 atcttatgtc ttggagcatt tggcatagtg ttttatctat tgaagaaaga agtgaaacca
     6061 ttaacgatct tgatcggact agctttcttt ggaatttttg gttcgtggat agggattttt
     6121 taaaaaagga gcagagaaaa tgtttacgat gcaagattat atctatgaag aaaaggaagt
     6181 actaagtacg atcttgaaaa agaatgattt ttcgacaaga gaacatatga aaaaaacagt
     6241 gaatttactg atcttagcaa cgggttcatc ttataacgct tgtttagcag cgaagccagc
     6301 attggaatct tatggtgatc ttactgtcga tattcaagaa cctttttatt ttttcaatta
     6361 tggaaaactt tctccttcaa tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc
     6421 ttctactgtt gaagcagtaa aaatgataca aaaacaaggg ttacctgttg ttgctatcac
     6481 aaatgacgtg caaagtccac ttgctttaga agctgcacaa attattgatt taggtgccgg
     6541 ggtagaatca gtaggcttcg ttacaaaagg ctattctgcg actgttttac agttactact
     6601 cttagggata ggaattggaa taagtaaaaa taaaatcagc aaaaaaatag aacaagacta
     6661 catgcaacag ttgcgcaaaa taatcaacca tttaccagcg attattcaaa aaacggaaga
     6721 attttttgat ggatatcaaa gtttatttcg tttagcacag cgtttcgtca ccattggata
     6781 tggtccaaac tggggaacag ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt
     6841 tccgtcacaa ggatttgagt tagaagctta tatgcacggc ccatacctgg aggcagatgc
     6901 ttcgcatttg ctattcttta ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt
     6961 gcaaagatat atgagtcggt acgttggaga gactctcacc attaccacaa aaaaagcaag
     7021 aaacgagaaa acacttggat tagcaataga atgtgatgaa tatctgtctg tattagcgct
     7081 tgtcgttccg tttcaactat tcgcttataa aaccgcagta gctaaaggta ttgacttgaa
     7141 caaaaagatt tttgaagact ttgatacagt cttgaaaagt aaattataag aggtggaaat
     7201 catgctgaaa ttcaatgaag aagaacaaat caaagcaata aaaggcgcac tagctttacg
     7261 tccgcaagta gaagaaatca tcgataaact ttatgcggaa aaatttgacg ctgtctatta
     7321 tctaggaatc ggtggaacgt atgcttcctc gatgcaagct gtcacttata tgaatgggaa
     7381 aagcaacttg cctgtctttg tacagcatgc tgcagaatat tacactactg gaaacaaaag
     7441 attaacaaaa gattctatcg ttattttgtc ctctgttacc ggtacaactc aagaagtagt
     7501 gaaagcagta gaacaaatca agaaagtagg agcgacactg attggattta tcgataaagc
     7561 gaacagtaag ttgtctcagc tatgcgattt tgtggtcact tatccagctc ctggaacaga
     7621 acaaatcaaa ttttttatgg cagcagatcg attgatgtat ttgaatggag aattcgaagc
     7681 ttattcagaa tactatgaac agctcgaacg ttatttacca acagggttag tggaagcaga
     7741 gaaaaaagca gatgcatttg gcttatcatt tgcagaaaaa catcgacgtg atagtatgca
     7801 ttattttatt ggagcaggga atcaatgggg agctgtttat tcatatgcca tgtgttactg
     7861 ggaagaacaa agctggcttc catcgaaatc tatccatgct gccgagtttc ttcacgggac
     7921 gttagagatc gtagaagaaa caaccccagt cacattattt ttaggcgaag atgaacagag
     7981 agtgttatct gaacgtgtgg ctaaactttt gccaaaaatt tgcagcaatt atacattgat
     8041 agacacaaaa gactatccag tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc
     8101 tttcttattg atgcatgtag tgacacaacg catcgatgct catgtggagc agttgaactg
     8161 ccatccatta gagatacgcc gctactatcg tcagtttgat tattaggata gactggataa
     8221 aaagaatata aaaaataaag agagcagcga taactgctcc ctttattttt ttaaaatatt
     8281 ggcatcaatt acgttataaa acacattttt tgcattatct acagttcaac ttataatctg
     8341 tttaatggga catgtgggtg ccaaccattt tttgttatgc aatagtacta tttagttgtt
     8401 gcatggatcc gtagtaaaag tccaagtaca acgagttgcg ccagtttaca tagcgttttg
     8461 tatccagaat gttgactaaa acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt
     8521 ccgttgcagg acctgacaga tttagaaatg ttcaatctgt ctcttatgta ttttaaatca
     8581 atactcacta ggagaagttt cctaacctcc taatagtaat gctggtagta acattgtcaa
     8641 aactgcttcc tcttaggaac aagcaaagca taacgagtgt aaaaataaaa tgtagactat
     8701 tgtaacatat cattgattat ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa
     8761 aacaatggaa attagtccca actttaagaa atgtatacta taatggaaat tatcaattat
     8821 ttatttggag gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc
     8881 actgctttag cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta
     8941 cctgaacaga ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg
     9001 acgatcccag aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat
     9061 gctattttat ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt
     9121 aaaatgaata ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat
     9181 aaacgaatct cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt
     9241 gtattatctg gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca
     9301 gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat
     9361 ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat
     9421 atcattgcaa ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca
     9481 gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat
     9541 cctctgacat ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt
     9601 cattctcgca attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt
     9661 ctagaaaaca tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta
     9721 gctcaacagc taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat
     9781 ggagaagata tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat
     9841 gaattttctc ttcgttaaat agatgatcaa tagttaatcg t
//
LOCUS       DAZAOG010000101         9859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000101 DAZAOG010000000
VERSION     DAZAOG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.101"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHDLD_002005"
     CDS             278..787
                     /locus_tag="ACHDLD_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3363691.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHDLD_002006"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHDLD_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3363692.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHDLD_002007"
     CDS             2322..3302
                     /locus_tag="ACHDLD_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3363693.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHDLD_002008"
     CDS             3424..4842
                     /locus_tag="ACHDLD_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN3363694.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHDLD_002009"
     CDS             4913..6382
                     /locus_tag="ACHDLD_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3363695.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHDLD_002010"
     CDS             6392..6859
                     /locus_tag="ACHDLD_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3363696.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHDLD_002011"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHDLD_002011"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3363697.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9859
                     /locus_tag="ACHDLD_002012"
     CDS             7848..>9859
                     /locus_tag="ACHDLD_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3363698.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAK"
BASE COUNT         3414 a         1431 c         1972 g         3042 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaagga
//
LOCUS       DAZAOG010000102         9820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000102 DAZAOG010000000
VERSION     DAZAOG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.102"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACHDLD_002013"
     CDS             complement(<1..195)
                     /locus_tag="ACHDLD_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363699.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACHDLD_002014"
     CDS             complement(211..825)
                     /locus_tag="ACHDLD_002014"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN3363700.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACHDLD_002015"
     CDS             complement(972..1655)
                     /locus_tag="ACHDLD_002015"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN3363701.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACHDLD_002016"
     CDS             complement(1764..2222)
                     /locus_tag="ACHDLD_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN3363702.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACHDLD_002017"
     CDS             complement(2306..4069)
                     /locus_tag="ACHDLD_002017"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN3363703.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACHDLD_002018"
     CDS             complement(4290..5750)
                     /locus_tag="ACHDLD_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363704.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACHDLD_002019"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACHDLD_002019"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN3363705.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACHDLD_002020"
     CDS             complement(6907..8334)
                     /locus_tag="ACHDLD_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3363706.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACHDLD_002021"
     CDS             complement(8365..9579)
                     /locus_tag="ACHDLD_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN3363707.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2913 a         2041 c         1627 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DAZAOG010000103         9653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000103 DAZAOG010000000
VERSION     DAZAOG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.103"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..699)
                     /locus_tag="ACHDLD_002022"
     CDS             complement(37..699)
                     /locus_tag="ACHDLD_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363708.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(712..2214)
                     /locus_tag="ACHDLD_002023"
     CDS             complement(712..2214)
                     /locus_tag="ACHDLD_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363709.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2762..4441
                     /locus_tag="ACHDLD_002024"
     CDS             2762..4441
                     /locus_tag="ACHDLD_002024"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN3363710.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4568..5047
                     /locus_tag="ACHDLD_002025"
     CDS             4568..5047
                     /locus_tag="ACHDLD_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN3363711.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5266..5946
                     /locus_tag="ACHDLD_002026"
     CDS             5266..5946
                     /locus_tag="ACHDLD_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN3363712.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5954..7285
                     /locus_tag="ACHDLD_002027"
     CDS             5954..7285
                     /locus_tag="ACHDLD_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN3363713.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7300..8262
                     /locus_tag="ACHDLD_002028"
     CDS             7300..8262
                     /locus_tag="ACHDLD_002028"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN3363714.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8373..8711
                     /locus_tag="ACHDLD_002029"
     CDS             8373..8711
                     /locus_tag="ACHDLD_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN3363715.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8790..9497
                     /locus_tag="ACHDLD_002030"
     CDS             8790..9497
                     /locus_tag="ACHDLD_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN3363716.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3061 a         1737 c         2076 g         2779 t
ORIGIN      
        1 tggttcggat tttttatttg atcattatcc tgttttttaa acttcgattt tttgaaattt
       61 tttatttttt agttcaaaga cttcatcaca gctttttgct acttcaatct catgagtcac
      121 gatgataata cagcgatttt gttcatgggc gatttcttgg aaaagtttga cgatatcttg
      181 tgaagttgtt tcatctagat ttccagtagg ctcatccgct acgattagtt gatgttcaca
      241 gcagatagca cgaacgatag cgactcgctg ctgctgacct cccgaaagtt tggtcacctg
      301 tttttttgct aatatttcat caattcctac acgagccagt cgatctaaag caacttgtcg
      361 ataattctct tgcttggtct tagtgatttc cattgctgct atgacgttgt ctagagccga
      421 taaataagga agcaagttgt aagcttggaa aataatcgag acatcatttt tacgataact
      481 tcttaacccg attttgttca gtgaacgatc ttgatagtat acctttcctt cttttggtgc
      541 atctagtcca gaaataagcg aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat
      601 agcgtacatt tttccagctt cgaaattcag ttctacatct ttataaagcg catcttgcgg
      661 atcttgatac caaaatccca attgttctgt ccgtaacatc aaattcctcc tttagtttaa
      721 taggattttt cttgggttta atcttaatat ccctatagaa gctaaaaaga cagaaaacag
      781 actgattcca aatgctactc cggctaatag tcccaattgt gcaggtttga cattgatttc
      841 caattcgctg acttgatcag aaacagcaaa tggattattc gtggttcctt gttgaccatc
      901 gcgttgaccg ccttgttctt gtctgttacc tggcatttgt ccaccgccag gagcttgctg
      961 gttttgtgtc gtggtttctg tctgttggtc aagtagctgc tgtcccacgg cattcgcgac
     1021 gacgtttccg ctaagcgaag caactcctaa tgcaagaatc atacaaatag ccacttcggt
     1081 aaacaattgg agaatgattt tacttctgga ttctcctaaa gatagcaaga cgcctaactc
     1141 atgccgacgc tcccgaatgg taatcataat gatcagagta agtatgataa taccggctac
     1201 agccactagt aaaacaacat ttttagaaaa acttgccacg ttgtttaaag gttccaacat
     1261 tgactgatac atggaatcat ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc
     1321 agcttctttg agaaactcat ccattttatc tggatcagat agagtgtaaa tagcagaatc
     1381 tatcgtgtta tcgtcagatg tcccatttag ttcatttgca aaagtgtaag aagtgtaaag
     1441 tgtattcgac ggattcatga agttgaattt catacccata ctgccgcttg tttcgctgga
     1501 ttcataaatt cctttgatcg tcatttcata agtggtatct tcatcttcag tactcgtgat
     1561 tgtgaaagta tctccgactg ccaggtcatt tgtttctgct agagtagaat caatgactac
     1621 attgtttgtg cctttgtcgc tgtcatctat tccttcacca tccgtgattt ttgctgttcc
     1681 atcagaaaaa ttactatttt gtgcagttgc tgacacacca ctgatttgaa agtctgcttg
     1741 agtcatttgc ggattcccgc ccattcctcc agcaggttca ccgcctgcat cagtagtttc
     1801 tgaactgtct tcacttgaat cagaactagt gatggcagtt attccatctt ttgccatagc
     1861 tgaggcggaa ctttcaaagg aataactctt tacattatct aattcagcta ttttttccgc
     1921 gtccgttaag gaaactggtg taagagaaaa actgcctgga tctggacgtt cttcagtggt
     1981 agaaccgctt gaagaagttt gttcctgttt tttaaatgaa gcttctcgat tggcacttaa
     2041 agtcactgtt gctccaactt ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg
     2101 aatcgtgagg ccagctagca caaaaataag aatggctgtg aacacggcaa acaataacaa
     2161 tgaacgtccc cattttactt ttgtactttt ccatgcacgt ttcataaaat tcatattttg
     2221 catccctcct agtagatgca tttgataata gacaacctat cttaaagcta gcttaaatct
     2281 tatgctgttt gcacaccatc aacatgaggt tctgtatata taaaaagtca ctatagttat
     2341 tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag
     2401 aggaatagca aaagaaatcc tagatgctac agagggattt ttacgtgcat agttgttcta
     2461 acaattgctg atactagcga tttagtttat ttgttgtata ataatgggga acagctgaat
     2521 acttatataa gtattctggc tatagcgttt cgcaaaagat ccattttgtt aatgaagtat
     2581 atccaaaata aaatgcccat ccaactgtca gaacgcggat gtgctggtaa ttttcctgct
     2641 gattctccat aagttgagaa atagtgattt tgcatatgct tcattggaat ggctcatata
     2701 tttagataaa gaagaaaatc gacatcttta aacttggatg tcataagaag aggtgaagtt
     2761 tgtgagtacc attaaaatta tcccattagg cggcgttcgt gaaaatggaa aaaacatgta
     2821 tgtcgcagaa gtgggcgagg atatttttat tttagattgt gggctaaaat atcctgaaaa
     2881 tgagttgttg gggatcgacg tcgtaatccc tgattttacg tatttagaag aaaacattga
     2941 tcgtgtagct ggtgtctttt taacacatgg acatgcagat gcgatcgggg cacttcctta
     3001 cttgttgtca aaaatttctg taccagtatt cgggacagag ttgacgatcg agttagcaaa
     3061 attgaatgtc agtcgtaatg aagcagccaa aggatttaag gatttccatg tggtagatga
     3121 gcatacagaa atagactttg gtgaaacagt agtgagcttt ttccgtacca ctcatacgat
     3181 accagattca gtggggatca atctgaaaac aaaagaaggt agtatcgttt atacgggaga
     3241 ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca gattatgcac gattggcgga
     3301 aatcggcaaa gaaggagtct tagccttact aagtgaatcg gcaaatgcag aaaatcctca
     3361 gcctgtagct tctgaagccc aaatagcaga tgaagtattt gatacgatca aatattggga
     3421 agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac aagtattgga
     3481 tgcagcagaa aaagcacaac gaaaagttgt cttgactggt caagatttcg aacgaattat
     3541 tcgcacagcg atgcgtttag aaaaacttca attaccaagt gaagatctgc ttgttacgtt
     3601 gaaagacatg aaaaactatg atccagaaga actgatcatt ttagaaactg gccgaatggg
     3661 tgaacccatc aagtctctgc aaaaaatggc aaacggtaca caccgtaatg tcaagattga
     3721 agaaggagat ttggtttata tcactactac gccaagcatc gcgatggaaa ccatcgtggc
     3781 gaaaacggaa gatatcattt atcgagctgg tggaacagtg aagttgatct ctgagaatat
     3841 gcgcgtttct ggccatgcca atcctaatga cttgcaatta atgatcaatt tgatgaaacc
     3901 aacatatttt gtgccagttc aaggagagta tcgtgaatta gctgctcatg cagatttggc
     3961 tcatgcagta gggatgccat acaaaaatat ctatattact ggacgcggag acgtgctgga
     4021 atataagaac aagcgtatga gtgttgcagg aactacaagt gcagaaaatg taatgattga
     4081 tggattaggc ataggtgata tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga
     4141 agacgggatt ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa
     4201 acctcaaatt acttcacggg gattcgttta cgtaaaagct agccgtgatc tgatgaaaga
     4261 aagcagcaat atgattgaaa caatcgttga gaaacattta gaaagcaatg attttgagtg
     4321 gagcaaatta aagcaagaca tccgagatca attaggccgt tacttatttg aacaaacaaa
     4381 acgccgccca gtgatcttgc cagtcattat ggaagctact caacgtcgaa ataaaaaata
     4441 aacaaaaaga gtaagatgaa cctctctata gagtgctcgt cttgctcttt ttttgcgaaa
     4501 aacaaataat ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag
     4561 tgaatcaatg atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta
     4621 tcaaccactt gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag
     4681 agggattgat ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt
     4741 gatcatcgca ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac
     4801 gaaagatcgt aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc
     4861 ccacggagca aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc
     4921 agacagttct ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag
     4981 ccttcggata aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat
     5041 tagttaaggg acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc
     5101 tgcttggtac ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta
     5161 tcaatccaat gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa
     5221 tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat
     5281 catggacatt cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg
     5341 tttattacag gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg
     5401 ctgattactc atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat
     5461 caagccacaa ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat
     5521 tcattcggta tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta
     5581 catgcacagc atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct
     5641 agcggaatta ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat
     5701 ttcagtgatc taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt
     5761 ggcgataact atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc
     5821 aagaaagtag ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct
     5881 tttgtagagt tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa
     5941 gtgtaggcag aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc
     6001 tgttggcaat cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac
     6061 agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt
     6121 agggaccatc cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga
     6181 ggtcgtcagg atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt
     6241 gaataaattc gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg
     6301 atcactaatg atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc
     6361 cgcagtattg atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga
     6421 tcttgaaatg ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa
     6481 ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat
     6541 gccagctgaa aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt
     6601 gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg
     6661 gattgttacg gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat
     6721 gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat
     6781 gatctgggat gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt
     6841 gattacccgg caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc
     6901 agatacgatt tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg
     6961 aaatcgtttg gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt
     7021 tggaaccatt tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat
     7081 ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct
     7141 gatccaattg gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc
     7201 cgctaaactg gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt
     7261 ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata
     7321 gtagatacaa taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct
     7381 gatgcattag gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa
     7441 gaaatacgtc aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa
     7501 gttgaagatg tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc
     7561 agaatcagtg atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc
     7621 aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag
     7681 atgattgcag aatttgcttt gatgtttcct gaatctttaa agatgaacgt taaggctgca
     7741 cggttacttt atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg
     7801 tcccgtacat tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg
     7861 aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa
     7921 aatatccagg tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat
     7981 aaatatggaa tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac
     8041 gaagtgctgg cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta
     8101 cgttctaagg ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca
     8161 ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc
     8221 caagatatat gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt
     8281 gccttttgta gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata
     8341 gtgctcacta aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg
     8401 gctcagagta cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat
     8461 ggaacaaaga agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg
     8521 gaaaccgtct gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg
     8581 cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc
     8641 ataatatcga ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga
     8701 aaaagaatta aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa
     8761 taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag
     8821 atccgacatt tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg
     8881 tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac
     8941 aagcagcaca atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg
     9001 ccggagatct cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa
     9061 ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg
     9121 tacaagatgc ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta
     9181 actttggttc gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact
     9241 ctacgttaat caatatcggg attttcgctt tttctttagc attacttttc caattggtga
     9301 cattaccagt cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac
     9361 tactgacgga agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt
     9421 atgttgcagc agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg
     9481 gcaaccgaag agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac
     9541 cgagagttag ctgattgaat accatttatt tggaataagg gctctttgtc aataaggact
     9601 gatgggttgt acaaaatcaa atctgggtca gaatgaacct cattctggct cag
//
LOCUS       DAZAOG010000104         9571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000104 DAZAOG010000000
VERSION     DAZAOG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.104"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACHDLD_002031"
     CDS             complement(<1..1128)
                     /locus_tag="ACHDLD_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFN3363717.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSH"
     gene            complement(2000..3631)
                     /locus_tag="ACHDLD_002032"
     CDS             complement(2000..3631)
                     /locus_tag="ACHDLD_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN3363718.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3645..5318)
                     /locus_tag="ACHDLD_002033"
     CDS             complement(3645..5318)
                     /locus_tag="ACHDLD_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN3363719.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5331..5648)
                     /locus_tag="ACHDLD_002034"
     CDS             complement(5331..5648)
                     /locus_tag="ACHDLD_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3363720.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5673..7073)
                     /gene="lacG"
                     /locus_tag="ACHDLD_002035"
     CDS             complement(5673..7073)
                     /gene="lacG"
                     /locus_tag="ACHDLD_002035"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN3363721.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7310..8095
                     /locus_tag="ACHDLD_002036"
     CDS             7310..8095
                     /locus_tag="ACHDLD_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3363722.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8098..8790
                     /locus_tag="ACHDLD_002037"
     CDS             8098..8790
                     /locus_tag="ACHDLD_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3363723.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9064..9435)
                     /locus_tag="ACHDLD_002038"
     CDS             complement(9064..9435)
                     /locus_tag="ACHDLD_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN3363724.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2889 a         2099 c         1682 g         2901 t
ORIGIN      
        1 atgactgcga cctaggaatt tttggttgta agtatcaaaa gacgagacta agaaacgttc
       61 catcgactct tcattttgga actgttcctt gcggtggctg tatttcttga tttgtttatt
      121 aaaggattca atcaagttcg tagaataaat actcttgcgt atggccaagg ggaaatcata
      181 gaaagtgagc aagtgatcgt tcgaaagaat cgattcaacc acttttggat agctggtttt
      241 ccatttttcc gcaaaagtac cacgtgcttc caacgccacc tctttagatg aggcttgata
      301 caccatttta aaatcatcac agacttcctt acgatcatcg acacgcactt tatgactgat
      361 attacgggaa acgtgtacac aacaatgttg aaaacgagct ttgggataga accgactaat
      421 cgctcctacc atccccttta agccatccgt gatgaacagg aggacatttt tcaaaccgcg
      481 ctcttgaagg tccaataaaa tttcctccca aatcgtgatg gattcagtcg gtgcaatcgc
      541 atagctcaat acttcctttg atccgtccgg gcgaatgcca actgcgatat gaatagcttc
      601 cttggcgacg gttttccgct ttaacggaat atacgttgcg tccatataaa tagcagcata
      661 acggtcatga agctcccgcc cattaaacgc cgttacctct tctgtaaatg attttgttat
      721 attggacatg gtttggggcg tgtagtaatg cccatacatt ttctcaatca agtctgcgat
      781 ttccgacatg gtaatacctt ttcggaagag gtgaatgacg gtctcctcta acgtgtcatt
      841 cgtccgtcta taagcaggaa cagtctgttg cttgaactca ccattgcggt cgcgcggaat
      901 ctggagatga agttccccat actcggtctt gaccgtacgg tcataggagc cgttacgaga
      961 attacccgtg ttaaaaccaa tgcgatcata cttttcgtaa tctaggaaag ccgttaactc
     1021 cgttttgaga agcgtattga ctgctttctc caaatggaca cggaataatt cattcaaatc
     1081 gcctttgttc gctagagtct ttagaatttc tgtagtaaaa tcgttcatag ggaagtcctc
     1141 tcttctgtga atgtgttgtg gtaactctat tctacagaag ggacttcctt ttttctattt
     1201 acacaaaata ttttacactc tccattataa caattgggtt aaaaagaaaa tagatagtat
     1261 tctacctcat atttatttga aagtaatata taaaagacgt tcaaattcga acgcctctcg
     1321 ttaaccaaat attggattca gaatctagtt ttcatatata aacagagtat gattaggtgc
     1381 aaaaattgca cttttattgc acttttattt ttatttatag tgttttactg ttctgtatat
     1441 tgttttaact cccttgatag tatcgcattc tgttttatac actttacgtt gaaaacagag
     1501 tgggaataaa gagccaacat aatatggagg gggaatccct ataaatcaac attcttcaat
     1561 agctaaaatg cttgattttc attaaaaata agatggttcc ctaccaaaag agactataat
     1621 ataagttaat ttgtttcatt cttatcataa aaagaagttc tcgaaaaacg aactgttttc
     1681 cgagaacttt tttctatcca tctaagtgct caaatcatat tcacgacgga attctaaggt
     1741 taccctttct taaacaaact ttatgttatc tgcacaactt tttgcaattt tttcattgct
     1801 tcttacgaaa tcgttgaact ctctttagca taatacaaag tgtagatgaa gtctggtgct
     1861 ttactcgttt tggtaaaaat gactggaaat cgtttctggt tatttttggc aaaggcgagg
     1921 tgcaggttcg attcctgcct tctacgctaa cgatgtggct gaagggaacc tccgatcttg
     1981 aatcgaaacg atccgcagtc tattgtttca ccaatgaaac aaaatcaacg ttcgtccaag
     2041 catttcctga agccttgcta taaaccccaa tttcacaaac acctgttgta attggaatgt
     2101 ctttgattgt aatttttgtc cattcacttg ttttaacaac cgcagtattt agttccgctc
     2161 caccataatt tttgatatat aactgattag tatcaaactc tcccccagac attacatagg
     2221 cactcaatgt gtaagttcct ttgcttatgc cactaacatt ttgataagct gatgcctcgt
     2281 atgcttgatc ctgccaaaaa gttagtttgt agtccccaaa aaaagtgccc gcttccgttt
     2341 tgatcgtacc cttagcggtt ccttgttgaa accaggtttg ccaattggaa ggactggtcg
     2401 ttggtccatc gctttcgaaa cttgcatttt taactagatt tatttgcgcc cctgcttgtt
     2461 taaacgcacg caagcttggc aaggcgtttc cagcaaaatc aaacatcgct tggttgtccc
     2521 attcgtttcc catagtggat tgaacactga gataattcat acctgcttgt gttgcccaac
     2581 tcacatttcc gttgtaccaa agaggttccc aatagaagaa gcccgccccg cgattattcg
     2641 gtactgcctg aatccgatcg gcaagatcca gcaaatactc tgcttgtcct gtcggtgttg
     2701 gttcatatcc ccccgcagct gcttcggctt caccaaaagc atttggcata gtatcggcat
     2761 tcgctgttgt atgcccataa gctgtttcaa ctacgatcac ttctttacca taccgttggc
     2821 tgatcgcatt catattggtt tgaagttcat tcattgaacc gtgccaatac ggataatacg
     2881 acactccaat taaatcaaat gacacacctc gactcgtgat ttcatcaaac caccaagtaa
     2941 atgctcctgt atcgccacca tcagctaagt ggagcattac tggagttgtt ttaggttgag
     3001 tgtctcgaac agcttggata ccagacttca aaaaagcagc caatcgatca aattctcccc
     3061 cgccttctcc ccaagacttg ccataaggcc agagcattcc gctattcagt tcattgccga
     3121 tctgaaccat atcaggataa acgtccagct gcttcatttc cgacagaacg ttctttgtat
     3181 agtcgtacaa tgccgtattc atttcttcaa aggtcagacc ttgccatgct ttaggaagat
     3241 tctgtttccc aggatcaacc caaaaatcac tgtagtgaaa atccagcagc accttaagtc
     3301 ccagattttt tgctcgtttt gcaaggtcaa tcgtcgtttc cagatcattt gtccctgctc
     3361 cataactttc tccattacta ttataaggat cctgccacaa acgcagtcga acatagttga
     3421 caccattatt ttttaaaatc tctaaggcat cttttgaaat atcgtcttca taatattttg
     3481 caccagattt ttccatatct gacaggatcg aaatatctgc tccgcgaata aattcgctgg
     3541 cagatgcttc aacagttttt gaaacagccc ccaaagcccc taacacagct gcaaataaaa
     3601 cagctttacg caaccaaatt cccattttca ttatggctcc tcctttattg aaattggttc
     3661 agaataaact gaacagcttt ttcaggatct ctcgttaatc cgatatattc tgctcctttt
     3721 gtcgcaacca gcttgattcc taagcgatcg gtgtcttctt tgatatcatc atagtaagaa
     3781 ttgacttgcg gtgctaagac caccatatca aaatcctgca gcatttcgta atgagaacca
     3841 taagcacccg ccattgcact gatcggtgct ttcgattcag atgcaccttt ttttaaagca
     3901 ttagctaaca tcgcacttgt ccctgcgcca gcacaaagta ccaacacatt gatttcttta
     3961 tttgcatcgc tgataacttg actcgctgcc acaggtactt cctcacgtac atcggcagaa
     4021 gcaccttcct cggttgcgat cacctctgct ttttgtgctt ctttctctac taagacagca
     4081 tcgtatgctt tgcaaaatgg tagatagatc aggaagtcaa cgacgattag aaccgctgct
     4141 agaacaaaag ctaagatacc catgcccgtt cctagaacca aaccgatcgg tgctggagtt
     4201 gcccaaggca aaacgtacat aaagctgttc atgcccaata catcgacaaa aaacttgaac
     4261 aggcaaacgt ttacaacagg tgttaacaaa aatggaataa aaaaataagg gttcaaaatc
     4321 atgggtgccg caaatagcaa tggttcgttg acagcgaaac ttactggaat aaaggaagct
     4381 ttcccaactg ccttcaactg cttagactta gcaaagagta ggaagataaa aggaacaacc
     4441 aatgttgccc cagttcctcc catagttcct acaaaattcc ctaaacccac cgtcagtact
     4501 ttagaagctt gttcaccgga attgaacaat tgcagattgg tttccacatt ggcatagata
     4561 attgccgcaa ttgccggctc cacgatcgac ggtccatgaa caccgacaaa ccaaaacagc
     4621 gccatcgctc cccagataat agcgattcct aagtacccgt cagctgctgt aaatagcggc
     4681 tgcagcaccg taatgattgc ttctgcaaat gagatttgga agatttgtcg aatgcctaaa
     4741 tcaattaaaa cggccgcaaa tactgaaaaa gagaaaggaa aaatatctcg gaaggtttgc
     4801 gaaatcgttc caggaacttc tttaggcatt ttaatggtaa tatctttcag cacacaaaac
     4861 ttatataaat taactgttaa aaatgcagcg acaaaggaag ccagtagtcc ctttgtcccc
     4921 atatattccg ttgaaaatcc accttcaatc ggagagacgc ttaacaacag gaaacttacc
     4981 acagaggcca gcatcacaga ggtggtgttg attactttgt ttttcggtaa gcgacgattc
     5041 atcgaatcag ccaagcagcg agcagtggtt gctgaaacga gtaaccctac aacacccatc
     5101 gaataattat agactttcca tagccatgtg gagacagatt caggccattg gtaaccaaaa
     5161 atctcaggaa ccgcagcaat caaaatgaaa atgcttgaaa ataaaatagc tggcatcgct
     5221 gttaaaaatc cgtcacgaac ggctcctaaa taactattac gtgagatttt ttcaaaaaat
     5281 ggtttccctt tttcgatttg tttaatgatt ccatccatag cgttcttctc ctatccttga
     5341 ttctgtctgt acagtacaat aaagtcttga atcagatctc tcagcaataa agtggtcatt
     5401 aaatgatcct gcccatggat aaaaatgaat cccatctcca tctctttccc agatgcttct
     5461 tccgctaaaa tttttgtttg cgaattatgt gcaagggtta aattttcgtc ggcttctttc
     5521 aacgccgaat caacatgttc aaagtttcct tgtcttactt ctttcaataa tttcaataat
     5581 gtactgcgag cgtcgcctga atatgcaacg atctcaaaac caagcatttg taattcctct
     5641 ttattcatct tttcttcctc cattatcact tattaaataa tcgttttggt ttcactgacc
     5701 aacttgtacc agtaagctga ttcttttggg taacgcttct gggtttcaaa atcaacataa
     5761 aacaagccat accttttgtt atacccattt gtccatgaga aaagatccat aagcgaccac
     5821 aaaaaatacc ccttcacatt taccccggct gttatcgcct tgcttaagga ttccaaatag
     5881 actctcaaat agtcgattct cggctgatcc ataataatgc catcttcaaa ctgatcttta
     5941 taccccatcc cgttctcagt gatatagatt ttgttgtaat gtgggtaatc gcttttaatt
     6001 cgcagcaaca agtcatatag accttctgga taaatcagcc agtcccaatc agttcgagga
     6061 attccttctt tataaatacg ttctccgatt cccttcactt tataaacaga ggtccctttt
     6121 tcacccgtac cgttatgatg gattgcattt tctccatcgt aagctttgac aaaatggcat
     6181 tggtagtgat tgatccctaa gtagtcatta cgcgtggatg cttttttcaa ttcaacgaaa
     6241 tcttcttcag gaaaatgata gctggcctga tttgcttcac atatctcatc aagagcagtc
     6301 aaggtttcag ttgaataata gcctaaatag gtagcatcta ataagaaacg gatcgacaat
     6361 gcatcgtcta aaaaggcagc atgcttatct tccggcgcgt ctgttgccgc atatttcgtc
     6421 tctaatgagt gaactacacc aatttcaccc ggaagttcgt tttctttgaa atagttcact
     6481 acacgggcat gagcaaccat catattatga agacaggcga cgatctttgt aaaatcatat
     6541 ttgattcctg gtgggaaaac acctaacaaa tattgattgg ttgcaactgg atagatttca
     6601 ttaaaggtac tccacacctt gacttctttg aattcatgaa aacaaaaaat ggcataggaa
     6661 acaaatgctt ctattgtttc tcgattcaaa aaatctccat ggtcaaacaa acgtttaggg
     6721 gtatcaaaat gatgcaacgt cacaaacggc gttacgtttc ttttcttaca ttcttcgaac
     6781 acacgatgat aaaaagcgat tccttctgga tttggtttcc cagctccatc aggaaaaatg
     6841 cggctccaag caatggataa tcttagacca tttacaccaa aacgttcaca caattcaatg
     6901 tcctttgcat attgatgata aaaatcactt gccggatcgg gactgaatcg cccttgttcc
     6961 tctaaaaagt catcccaagc aacagcgcct ttgccacctt ccttagtggc tccttcaacc
     7021 tgataggctg ctgtggctcc gccaaaaata aaatcttctg gtaatttaag catgtacatc
     7081 tctcctcaaa tctataatca gttttcgctc tttctaactg attgcattgt aaacgcatcc
     7141 aaaacatttg tcaacaaaaa caaacacaaa taatcataaa aaccagaaaa cagcaataaa
     7201 atctattgta acaaacataa aaaccaccct aaatgtttac ttttgttcga attagattga
     7261 ctgttgtccg tttatatgat agattatcct taaattagag gtgaagacaa tgttgaaaaa
     7321 agaaagattg cttactatcg tagaaatggt caataaaaaa ggaatcctta ccgttaatga
     7381 aatcatcaat caattagatg tttctgatat gaccgttcgc agagatttag atgaattaga
     7441 aaaatcgggc aagctgctgc gtgtccatgg aggggctcaa agtctttcct acactttaga
     7501 tcaagaactt tctcacactg aaaaagccac tctgcagatt caagaaaaaa aagaaattgc
     7561 tgccacagca gcctccttga ttcacgaggg agaaacgatt ttcttaggac caggtacaac
     7621 aatcgaacta ttggcgacgc atttactgaa caagaaaatt cggatcatta caaataatta
     7681 tccagttttt gatattcttg ctcaagcaga tggaccagaa attctactga ttggcggtga
     7741 tttccggaaa aataccggcg catttgtcgg accgatcacc aacgacaatt taagaagact
     7801 aaagtttaca aaagcattta tcagttcaaa tggcgttcac aacgaagcga tctctactta
     7861 cagtaccgaa gaaggagaag cacagcagat cgctctaaat aattcccgta agaaatatct
     7921 actcgttgat aataaaaaat tcaatcgaga tgatttttat gtgttttata atttgcatga
     7981 tttcgatctg ctcattacgg atcgccagat ctcgcgggag gttcaagagc actatgcaca
     8041 atatgtcgat ctgcaaattg ctgattcaac cgaaaatacc actataggag cgtaactatg
     8101 aaagggatta tatttgattt caacggaaca atgtttttgg actctcatct gcatgaagct
     8161 gcttggctgc atatgataca tgatcataca aaagatggtt tatcagacga ggatatttta
     8221 aaaaatattc atggtcggac gaatacggag attttgaaac atttcatttc agagcatttg
     8281 accgcctctg agatcgccgt attgtcagaa gaaaaagaaa gctattatcg tatgctttgc
     8341 ttgcaaaatg aaaacgagct tcagttgaca aaagggcttg aagcgactct tgaccagctg
     8401 actgaccaac agattccatt aacgattgcc acggctactg tcaaagagaa tgttgctttt
     8461 tattttgata ttttcgattt agcccgctgg tttgactggg acaagattgt ctatgacgac
     8521 ggctctttcc ctgggaaacc gcaaccggat atttttctta aagcggcaga caaactgagc
     8581 ttagctccgg ctgattgtct tgttatcgaa gatgcttact caggattact tgctgccaaa
     8641 cgtgcgggga tcggaacaat tattgcaatt gatccattcg gtaaaaacaa aacagtattt
     8701 gaacaggcac agctaaataa agatggaacc atcaaagatt ttactggttt ttggacagag
     8761 cacctgtctg ccacatccgt taattcttaa acgattcaac taactaccaa agaaaagcaa
     8821 ctgaaatgca cacctccttt tataaaaaac tgccagtgaa acgacatgaa agatacactg
     8881 ctatatcctt agtgaaatat agcagtgtat ctttgacttt ttcattcttt taaccatatg
     8941 acgcgttcat ctctatctat aattaacatt aaaaatagtg ttttcacatt atttctcaaa
     9001 ccaatcttaa aaatcatcag gagtactttt ttgatatact tttattaatg ctgggcgtaa
     9061 tggttaaaag atttccacta gttcctctcc accaagctga gttgaaaaat gcttatctgt
     9121 aaacaacata gacaaagagg aaataaacgt ttccgtatct ttctctgtcc caaggatatt
     9181 aattaatttt cctactaggt aagtatcatt aaacttaaga tctgccttca catcaaaagt
     9241 tttgataaaa gcttttatca tgcgtacaat tttgtcttta tcgccataat tgatttcacc
     9301 actcatatca atatagtcac taatccctga agaaataatg gcaactgctt cttctttcgc
     9361 cttatcagat aattcattta atgactggga ttgattgatg acttcttgag ttttatctgt
     9421 tacgtaatct ctcattatcc tatcggcata ctccgcaaag taatcttgaa aaaattcttc
     9481 tacagggtaa ccaacttcgt tttcccaacc aggagaaatt tcattatcag tacttattaa
     9541 atctattaaa aaccaacccg ttctctgagt g
//
LOCUS       DAZAOG010000105         9546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000105 DAZAOG010000000
VERSION     DAZAOG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.105"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..977
                     /gene="gnd"
                     /locus_tag="ACHDLD_002039"
     CDS             75..977
                     /gene="gnd"
                     /locus_tag="ACHDLD_002039"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN3363725.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            979..1980
                     /locus_tag="ACHDLD_002040"
     CDS             979..1980
                     /locus_tag="ACHDLD_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN3363726.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1977..2261
                     /locus_tag="ACHDLD_002041"
     CDS             1977..2261
                     /locus_tag="ACHDLD_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN3363727.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2294..3091
                     /locus_tag="ACHDLD_002042"
     CDS             2294..3091
                     /locus_tag="ACHDLD_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3363728.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3105..3938
                     /locus_tag="ACHDLD_002043"
     CDS             3105..3938
                     /locus_tag="ACHDLD_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3363729.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3956..4813
                     /locus_tag="ACHDLD_002044"
     CDS             3956..4813
                     /locus_tag="ACHDLD_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3363730.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4835..5344
                     /locus_tag="ACHDLD_002045"
     CDS             4835..5344
                     /locus_tag="ACHDLD_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN3363731.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5381..5782
                     /locus_tag="ACHDLD_002046"
     CDS             5381..5782
                     /locus_tag="ACHDLD_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363732.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5894..6247
                     /locus_tag="ACHDLD_002047"
     CDS             5894..6247
                     /locus_tag="ACHDLD_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363733.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6281..6724
                     /locus_tag="ACHDLD_002048"
     CDS             6281..6724
                     /locus_tag="ACHDLD_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3363734.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6741..7826
                     /gene="uxuA"
                     /locus_tag="ACHDLD_002049"
     CDS             6741..7826
                     /gene="uxuA"
                     /locus_tag="ACHDLD_002049"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3363735.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7819..8532
                     /locus_tag="ACHDLD_002050"
     CDS             7819..8532
                     /locus_tag="ACHDLD_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3363736.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8739..8888)
                     /locus_tag="ACHDLD_002051"
     CDS             complement(8739..8888)
                     /locus_tag="ACHDLD_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363737.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9284..9484
                     /locus_tag="ACHDLD_002052"
     CDS             9284..9484
                     /locus_tag="ACHDLD_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3363738.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3209 a         1498 c         2116 g         2723 t
ORIGIN      
        1 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
       61 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
      121 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      181 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      241 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      301 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      361 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      421 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      481 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      541 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      601 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      661 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      721 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      781 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
      841 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      901 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      961 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
     1021 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1081 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1141 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1201 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1261 caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat
     1321 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1381 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1441 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1501 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1561 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1621 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1681 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1741 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1801 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1861 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1921 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1981 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     2041 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2101 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2161 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2221 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2281 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2341 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2401 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2461 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2521 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2581 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2641 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2701 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
     2761 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2821 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2881 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2941 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     3001 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3061 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3121 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3181 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3241 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3301 ggggtttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3361 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3421 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3481 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3541 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3601 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3661 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3721 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3781 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3841 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3901 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3961 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     4021 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4081 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4141 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4201 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4261 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4321 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4381 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4441 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4501 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
     4561 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4621 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4681 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4741 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4801 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4861 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4921 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4981 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     5041 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5101 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5161 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5221 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5281 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5341 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5401 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5461 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5521 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5581 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5641 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5701 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5761 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5821 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5881 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5941 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     6001 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6061 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
     6121 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6181 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6241 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6301 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6361 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6421 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6481 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6541 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6601 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6661 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6721 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6781 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6841 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6901 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6961 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     7021 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7081 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7141 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7201 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7261 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7321 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7381 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7441 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7501 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7561 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7621 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7681 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7741 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7801 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7861 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7921 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7981 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     8041 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8101 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8161 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8221 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8281 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8341 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8401 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8461 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8521 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8581 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8641 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
     8701 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
     8761 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
     8821 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
     8881 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
     8941 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
     9001 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
     9061 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
     9121 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
     9181 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
     9241 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
     9301 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
     9361 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
     9421 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
     9481 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
     9541 aaattc
//
LOCUS       DAZAOG010000106         9489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000106 DAZAOG010000000
VERSION     DAZAOG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.106"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1442)
                     /locus_tag="ACHDLD_002053"
     CDS             complement(78..1442)
                     /locus_tag="ACHDLD_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3363739.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1439..2998)
                     /locus_tag="ACHDLD_002054"
     CDS             complement(1439..2998)
                     /locus_tag="ACHDLD_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363740.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3054..5534)
                     /locus_tag="ACHDLD_002055"
     CDS             complement(3054..5534)
                     /locus_tag="ACHDLD_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363741.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5518..5853)
                     /locus_tag="ACHDLD_002056"
     CDS             complement(5518..5853)
                     /locus_tag="ACHDLD_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363742.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5867..6901)
                     /locus_tag="ACHDLD_002057"
     CDS             complement(5867..6901)
                     /locus_tag="ACHDLD_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN3363743.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6991..7671)
                     /locus_tag="ACHDLD_002058"
     CDS             complement(6991..7671)
                     /locus_tag="ACHDLD_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3363744.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7955..9058)
                     /locus_tag="ACHDLD_002059"
     CDS             complement(7955..9058)
                     /locus_tag="ACHDLD_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN3363745.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         2731 a         1932 c         1684 g         3142 t
ORIGIN      
        1 tttcattata tgaatttttt attctcgatt gattaatatt tattatatcc ccacacaata
       61 aaagaccgtg atatttctca cagtctttta tctactctat tgctatttgc tctcttagct
      121 agtactttaa aagctaactc attgaggtag tgaaaacgag aggtgtgttc agacagaaat
      181 ccaaaatcct caactgctag ctgtggaatc gtagttttgt gtttcacgat atccgcaagc
      241 aggttcaaat ctgataataa gaattcttgg cttatcagct gtccgccaat tatttctaat
      301 gtatccggat ggtagatcag ctttgcattt actgttttgt tttcttcatc gtattgtgga
      361 aatagatcaa attctcctga acacgaaccg gtattttgtt ccaagaatgg cgcttcatct
      421 tctgtcagcc ctacagttcc taaaaagtag tctataatga ctgctgctgt tatcttttgt
      481 ctgaggtcgt aagcggttgg ctgaccactc aaagttagcg ctactgctct tgccatatgt
      541 acagctcgag taactaaagg taaaaagaga tgccgtttat ttttgtcaaa actgactggg
      601 acgatatctc caatcgcata gatgtccgga tcacttgtct gcagatattc attgactatc
      661 acagtctcat cctcatacaa agccactttt tcttttaaca atgtcgtatt aggacgagtt
      721 tgggcagaaa aaatgaccat atctgccttt attgcttgag atagaagttg tacggatact
      781 actttctctt ctgatttttt ccaatcgatc aagaattcat ttggataaaa attgatttgc
      841 ttagtttgca gctgattttg aaaactgttg atcatatcct tatcgaaata tcttgataaa
      901 acattcggca tataatcaac tagatgaata atgtatcctt tatttctcaa agcatctgct
      961 aattccaatc caatataacc agcaccgaag atcactactt cttttgcttc ttttaaagcg
     1021 gcaagtactt gcttagattg cactagattt ttataggtgt agacattttc agcttcaatt
     1081 tgtttataca aggtgacagg agaggaacct gttgctacga ttaatttatc ataggttatg
     1141 gtctcgtttt ttccttcttt ggatgcgagg atgagctgat gtttgtctgg atcaagttca
     1201 gttactctcc attcggtgat cagttttgcc ccactttctt gaagagtccg catgttatgg
     1261 gcaacttcag ctaaactacg tatctttcct tttaaataaa ggttgattcc atttgaaaca
     1321 tagcctagtc catctttatg atcttcatcg atcagtaaaa cttctgcttt cggttgaagt
     1381 tttcttagat ttagggcagc tgtgatgcct gcatgggaag caccaatgat cacgattttc
     1441 atgttttatt catccttaaa ttcaattttt ctaaataaca ttcttttaaa aattggttca
     1501 aataggggaa atcataatta tattcgttgc tagtgaatac aaaaacttta gcggacttct
     1561 gtgacgggta ctctttcaat tggctagtca aaatgatatc atactgcttt tctttctcgt
     1621 attcgactaa atcatatttg atcgtattca gcaattgttc ctggatgatt tctttcacga
     1681 tatccgccat tagcctttca taaggaagat cataagcaat ttgcagacgt aattctaact
     1741 gcttgatcag cgtccgcaaa atagatgcat agcgagcaaa taattcaaag cgggcatttt
     1801 cctcgtctac ttcagtaaaa tcaacaatag caaatgtttg tctgaccaat ctttcacata
     1861 aaaccaggaa ttgtttatct tctacttcag ttaaccgctg ttttaatcct tcttggccaa
     1921 aaatattcat ccatcctcta aaataggcaa aatgagcgtg attttgcatc actgtcaacg
     1981 caatcttttg cgaaatctct tctggaaaaa cataaaaaga gaaatattct tctatcgatt
     2041 gtttaaacgt tcgattcaat tgagctacta cgtcgtcttt gtatttttcg cttatcaaaa
     2101 actcctggac atgcgagtta tgtgtatcta atgtaaagtt actgatcatg aaaagataaa
     2161 aaaccaacgt ttcattttga ttccaaggaa aggcatattg aaagaagtaa cgggtcaaaa
     2221 atgtatggag gtccaataaa aattgtgtcg tttgaaacaa tccaacagga agattgctat
     2281 aatctggttt ggattttttc gtacagcgca ttcgcttctt cataatggaa agccatattt
     2341 caaatttcaa tcgtccttct tctgtaatag taaaactaaa ctctttttgc agcagatcta
     2401 aaaaagtgtt tcctgataaa tcactgtatt cttttttgtt ttcaagataa ggacgtccaa
     2461 accaaaataa ttggaaataa aaataacaaa tttgactttc tggaccggtc agccgcccgc
     2521 tttttatttt cagatgaaac tcatttaaag aatcatttaa ttccttcact ctgcgagaca
     2581 aagtagccga actgatatta agttccatga caagtttctg ccgatcaaat gagccatttt
     2641 ccaacagata aagaagtatc tgatattttg tagaatagcg caagtagcaa tgataataaa
     2701 catcgactgc tgctatattt ccatgtctga ttaataaaat attcccttct tggataacaa
     2761 tttccaattg tttgatattt tccaagttag cttgtaaata aaacagatct tctttcaagg
     2821 tagcccgatc aactgataag cgggtaagta cgttattaac ggaaatctct cctttttcct
     2881 ctaataaaag ttggaaaagt ttgatttgcc gttgttcatt tttttcaagc aaactgaaaa
     2941 gttccaagtc cactcctcca agcatttctg attttaacag cagaagtttc cttctcatcc
     3001 ttgaaaaaga ggatctgtga cagttatcac agatccatac aaattctaat cacttagctg
     3061 gttggcccag ctgtcagttc ccatgtgacc atgccttgat acgttgcgcc aggttgagca
     3121 ttcgtactgc tatttacctc taagagtaaa ccatcggtat cgggaaagtc aacgagaacc
     3181 tctcctttag ctgttccatc actctcataa atcaacgtgt tttgaccttg ctgaatcaat
     3241 tgttttgatc cagaatgatt gaagtataat tttgttccgc ttaattcttg accttgatca
     3301 ttcttaaata aagaagtcaa tgtccctgtc agttgccaag gagacgtgct tggaagccga
     3361 gtatcccgca catcaaaaga aaaatcgcct tttgcttcat aataacttgt cttcaacggt
     3421 ttggggagat cgttaccaaa tgtaaaatca tctggtacat taactaatgt taattcttgt
     3481 ttggggacaa gaattgtttt agcagatgtt tggacaactg ggttaggaag cttagcacca
     3541 tctgctaatg taatattgct ggaaagtaca gctgcctttg tcagtatgct gacggattgt
     3601 acagaagtat ctacagtatt taattgatag ttgtaagtaa gatgaatggt atctcctatt
     3661 ttgctcaata gatctgctgg gacagaaact ccttgttgaa tgccggtatc tgcgatagtt
     3721 gtaccattta attttaagga accattgata taagtagtat tttcaggaag tgagatcgtg
     3781 atttttttgt cggttatcgc atccccagat tttgttaacg tgaatgtcac attttcttga
     3841 atcgtatctc ctgatagagc agacagggga tctcctgtct caggttgtct gctaaggttt
     3901 tttagctgac tatctgcact cacagatgga aaattagcca ctgttatttg aggaagagtg
     3961 gtgattccag cttgttcaga ctgccatttg tacgcaaaag acggatacat gttagtaagt
     4021 acatcgtcca ccaagcgata atcaaaataa tattgccctg aagccaatgc aggtagtgta
     4081 gaagtagctg tatttacacc cccgttattt gattggatcg tttggaaaag ttgatctgtc
     4141 catcctgtcg ctaatttttg ggttcctttc gaataaagcc tgtaatggat cgaacccttt
     4201 tttagttcag ttgctgacca attggccgtg acttggtacg atgcagaaga aaggagcttt
     4261 ccccactgac tatcggctgt taagtttgtt ggtaaaaccg ttccgtctag cttgtactgc
     4321 tccaatacct ttaaagcaac aggagaaagc aatccgcctt gcgcatcttt cccactgtac
     4381 acgatattgg ctgtctgatt caatccactt gtgcttgctg gagtatgagt cagtattgct
     4441 ccttgtgtaa cgttttttcc tgtaaaatag ttgactacgc gatcaaagcg actaaatggc
     4501 aactgatcaa aacgtccttc agtcagcccc atcgtcaatg tccgactatt ggtagagcct
     4561 ggaaggttct ggttatttcc atccacgtat tctgtggtat cagcaaatgt attatatttt
     4621 cctgttgaat cacttgttga gatgtaacta ttccacgtat ttatctctgc acttgcagaa
     4681 aatgcatatg ctcctggatt caagtttccg ccagttaatt taggataaaa tctacctgca
     4741 gctggagcta aaatcgaatt gaacatacga tcttggataa tattattcga atcataaaaa
     4801 tcaacagaaa aaacggcctt tttagacata gaaggttgta atttaccctt ggtcaaaatt
     4861 ttgtttggac tcgtatcata tttatctccg ttttctgtca tcttcgcgac attagctgta
     4921 acatcaaatt gaagcgggat atcgtccacg ttgaattcgc ctcgcgtaat aatagcaata
     4981 atatctgcca atagagtatt tgtatcccac gaccccatcc caaaacgcaa ggtatggctt
     5041 cctgctgata ataaactacc gctagtaacc ttgatggggg caccggcaat atacaagtag
     5101 gcgttgttcc agtcaattgc ttttaataca tcactgcttg atacatgttc cggcaaattg
     5161 atgatcgcca caaatgcaaa tccttgtgca gtgatattca ccaaatcaag tacatttgtt
     5221 agtttcacat ggctttccca atgaaaacgg accgtgccac ctttccaagg atcatttgaa
     5281 ccatcgttca aataagtagc agtcaattta ggcaattggt tagccccagt tagttggaca
     5341 agcttcccgc cattgactgc actacgtcca ccatttggag gtacgatatt ttcatacgtg
     5401 tatggatcat aagaaatggc aggaggcgtt gctccaacag cttctgccga aggtgaaaag
     5461 ataaatatac ttaaaacaaa aagcgaagta aaaaagaagg agcgaaaaaa tgaaaattta
     5521 ttttgtagca acatgttcat cctccttttc ctttcttctc cataacaaca accacgcaag
     5581 aagccataat tctataccaa tgatcaaata aagaaaacgg atcttttctt cattcgtctc
     5641 cgggaatacg ccatgttgat tagattcttt ataaatcggt agctgtgtct gctgttttga
     5701 tgcgccattg actgaaccac ttggatgcgg aactggactt agatcataaa atgtaatcgt
     5761 acctgaactt ttggtatctg cttggactgg ctcttgccag ctgaagaaca gcaaacttgc
     5821 aataatccct cccaataaga tcgatgtttt caacgaaatc acctcatcat gttgtttctc
     5881 cttttttctt tttcttgtgg acccacaaaa acagcaaaat caaccataac actagaactg
     5941 cagcaaccaa tcctgcataa agccatggga aagattgttc ttgtactgca aaaacggaat
     6001 tttcatttac tttttgtgct ttttcttttt tgatcgtaaa gttttgtgta aatcgccatt
     6061 cattactgtc gcctgatttt gctatgattt ctgcgcggta atcgccggct tttgctttat
     6121 caccagctaa atcaagattc aagtgaaaat tcgaatttgg tgcaactaaa aattcattag
     6181 acgtattttc aatataaagc tgattttcat aaaagatctt gctcgtaaat atcaaattgc
     6241 tgataatcct aggctgagga ttttggaaac gaagctgaac agttggcttt gcgttgacct
     6301 cgtccaaatc tcctgataaa agtttcagat ccggttctat cgtctcttga ctttcttgta
     6361 aaacgactgc gagattataa gcaatacgat ttttgatttg tgctccttct gtagcatttt
     6421 ctgtttcagc ttcttcttta ggtgaaacat gaatacccgc taatacacgt cccttaaagc
     6481 tttttgctgg gattttcact tctataggta tagttgtttc tgcatgtgct gatacattga
     6541 tttccttttt cgcaatgagc aaaacgtcac ggacatcaaa cggcaatgtc tcatctaacg
     6601 ttgggttttt ttgactataa tcgatcgttc caccatcact cgttaccgcc ggattgacag
     6661 agacctcaaa tgttcgttct tcatctgaag aattgctgat aattacttct agtgtctgat
     6721 tttgttccgg tgccactaat aaatcaaaat aaccgattgc tttatctatt tggttctctg
     6781 gaaataccgg tttgattcca aagtctcctg ctgaatcctt tgcttggctg attgacggaa
     6841 aaatgaaaaa tagacttaaa atagaaaaaa acgaaacaaa cagtcgttta ctttgattca
     6901 ttgtccacca catcctttca acaacaactg cggtaaaaaa tccttcacct atagtatttt
     6961 caccgcagtt tgttgaattt atttcatact ctatgctact gggccagcca cgaggttcca
     7021 agtcaaatca gccgtgaatg ttccagctgc tgctttcggc actttcagtg tgatatttgc
     7081 tggatcataa acagaaagcc atgttcccag accttgatct tttgctgctg taaatattgg
     7141 tgtcgctgca cttgctttac tcaacccagt tacagtttga ctagttggtc cagtggattc
     7201 gttatctgac gtacttttaa cagtggatgt tccaaaatct aattcagcgc cttgcaaaac
     7261 ttgtgaaccg tttacaaaat ctgaaaccga aactgtcacg ttccaccctt gtgcttggcc
     7321 atctgcacct acacctcgac gatctgatac ttggagataa ggtgtatcgt tttttgaagc
     7381 gtctacttga tacgtttttg ttccgctttc aatctcgtgt gtaccaaatg gcaattcagg
     7441 aactacatcc aatgttaaag aacctgtctg gccagttgct ggattttccg gatctgctgg
     7501 ctctactggt tttgaaggat cagtagggtt aagaggctca acggtatttg ttggttcagt
     7561 aaaggtaata tctccttttg aagtcgcttg accctcagca aaagctggtg ttgccaagcc
     7621 gcctagaaga agtgttgaaa gtaataatgt cccaatcatg ttctttttca taataaatca
     7681 ctcctttttc ttagtgacct aagtatagta gagacagtat gcaaaaaatc ctcatgaatt
     7741 catccgctta cttgaaatta tgaccctttt tgtataaaag agaaaaaaac aataaaatcg
     7801 tttacatgaa tattcttcct tttttattaa gtatgaaaaa atatttcaat tttatttttt
     7861 cgtttcaaat tattctatta gtgacatcct tgaactataa aaataggagt acggcatcat
     7921 ctgccgcact cctatttttt tcgtcattta tttcttaaat aaaacgattc tcacgcaatt
     7981 ttattttttt gatatcttct actgtttgtg ttccagctaa ttgcatgacc atttccaatt
     8041 ctgttttaaa gaaatcaaac acttgatgga ctcctgtact tcctcctaaa gacaatccat
     8101 aaataacggg tctgccaata gcaacaagat cggcacctga tgcgattgct ttgaaaacat
     8161 gttgtccacg tcgtacaccg ctgtcaaaca cgattggtac acgtttatct actgcttctg
     8221 ccacatattg caacgaatca aacgcagcag gccccccatc taattgacgg ccaccatggt
     8281 tagataccca aattccacca gcaccagatt ctaatgatcg atagacatct tcttctgatt
     8341 gaacaccttt cacgtagact ggcaaatcag aatgagcagc gataaattct acatcttttg
     8401 gactaagttt ttgtttggaa gatttatata cagcatccat cgtttggcct actcctgatt
     8461 gataagcctg aacgatcggc attggcagcg ggaaggtaaa cccgtttctg cggtctgttt
     8521 cacggtttcc gccaactgtt gcatccgctg tcaatacgat tgcttttgct ccattacgct
     8581 tggccacttc aagaatatcc aaattgattc cattatcttt gctcatataa aattgaaacc
     8641 attgcggtgc tttctccccg cctgcttctc ttatctcttc taaagtacaa gaagcgtaag
     8701 aacttgctgt atagatcgtc ccaaaatctg ctacaccttt tgcagaagct ttctccgcct
     8761 tcacatgagc taatccatga gcagcaactg gtgccatgat gattggtgca gtcagcattt
     8821 cttcgtcaaa atgtgtagtt gtatcgggta attctacatc tcttaataca tgaggaataa
     8881 tcaaacgatg attgaatgcc cgttcatttt cttgataagt aaataagtcg ccagcaccac
     8941 tgctgatgta accataacca ccagctggaa tcacttgtga agccgcgatt tctaaatctt
     9001 ccatattgat aaaatctact attccttcat ttgtacctgc ttgatatgtt ttttccatct
     9061 caatgccttc tctctataaa atttgaatga tgaacaatat gatagcgttt acacttttta
     9121 tattactctt cgacattgat acaggcaaat aatctgttta catttcagcc attccagatg
     9181 tcactactct ggaagaaatg attgtaataa aaatagataa aaaaccgatg ggactttgac
     9241 aaaagtagtg tcaaagtccc tttttttgaa taaacggtgt tcaaaagcca ttttctcggt
     9301 attaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttactact
     9361 cgaaactaag cgactttttc acaacctctc gataaaacgg tgttcaatca gctgaccctc
     9421 ggtattagct caaaaaatgg caaaatatga ggaactattt tgccatttgt ctctctaata
     9481 ctcaggtca
//
LOCUS       DAZAOG010000107         9386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000107 DAZAOG010000000
VERSION     DAZAOG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.107"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..422)
                     /locus_tag="ACHDLD_002060"
     CDS             complement(<1..422)
                     /locus_tag="ACHDLD_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3363746.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(412..2586)
                     /locus_tag="ACHDLD_002061"
     CDS             complement(412..2586)
                     /locus_tag="ACHDLD_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFN3363747.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2602..4944)
                     /locus_tag="ACHDLD_002062"
     CDS             complement(2602..4944)
                     /locus_tag="ACHDLD_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3363748.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4966..7509)
                     /locus_tag="ACHDLD_002063"
     CDS             complement(4966..7509)
                     /locus_tag="ACHDLD_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3363749.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7519..7917)
                     /locus_tag="ACHDLD_002064"
     CDS             complement(7519..7917)
                     /locus_tag="ACHDLD_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN3363750.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7998..8282)
                     /locus_tag="ACHDLD_002065"
     CDS             complement(7998..8282)
                     /locus_tag="ACHDLD_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363751.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8306..9304)
                     /locus_tag="ACHDLD_002066"
     CDS             complement(8306..9304)
                     /locus_tag="ACHDLD_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3363752.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2675 a         2005 c         1468 g         3238 t
ORIGIN      
        1 taaaaattag gaagaaattg attcaatgtc ttttcaaaaa agaatgtttt tctctcaatt
       61 tttttgacgt ttctatcatg aatcacgaga aaacttggga cacttttaac atggtaacgt
      121 tgtaaaaaag tttgaagttc ttcttgtctt ggttcttttc ttgtttcaat tcgatataca
      181 ttcctagaga attttttgtc gtatctttcc aagaaagatt caacttcttg gcatttttta
      241 caatcatttc ttgctagata cacaaaaaat gattgattcg ttttttgaag ttctagtact
      301 tcttctaaat tactattgat tagttttcca tttgattctt cgttagtttt tatcagttta
      361 aaacaaccaa ataagaggaa actaacaata agtatcctaa caataacttt cttatttcgc
      421 atattgtttc tccaaatttg gtttccgaat atagacgact cgcggtggtt cctggacgtc
      481 ttcagctgtt cgatacacca caaattcgcc tgttctcaat gctcgaatgc gattcggatt
      541 gaatttaaag cggtctacgt ctcgtttcgt gcctctttct cctgtccaat caacctttcc
      601 catatcagaa taacctgctt cttgtgtcgt catttccgtt aattcaatgt cttcttctgt
      661 ccccattaaa tctgcccaat aatcaatttc tgtcgggtca tttgtcttcc caataaagaa
      721 cgtattcaca ttcccaatca actgttttcg aaggacagga tcaattctat caatgtccgc
      781 aagcgtttga ggggcatacg ccgcatgcac cccagcgcct cgtcccttat tgactaagtc
      841 tataaggtta tcatttagat aagaagctgg ttcgtcaaat attactagga ctttcgagcg
      901 attggctgtt tctcctgctt gtttatacac ttctgacgca aagaaattaa tatctcccac
      961 aagcatttga gccaatgtcc gaataaactc actataaatc aatccgttta acgagatata
     1021 aacaatcttt ccttctttaa caacttcttg aatatctaat tctctttttc ctccagaagt
     1081 atcaaagagt tcaccaagct cactatccaa taacatattg atattggtct gtaaattctc
     1141 agaagtcgca tacaaataaa acgggttctt cttatgttcg taacgaacaa acaaccgttt
     1201 aaacaacgtt tgctcgattt ctgtcatgag agaaatgttt tcttttaata atagataata
     1261 cgtttctaag tcaacggttt tacgatcatg ttcaacgatt cgctcttcac ttaactgttc
     1321 cacatcggca aaatcacgtt ctgttttgtc catcgcttca ttctttttcg tttttttctt
     1381 cgtggttttc ttcttttcta cgaccacttt ttctttaaat aaatctggat ttttcaactg
     1441 aatacgatcc gcaaacaaat gtaagacgtt tcgtggcaat aaataatgtt gtaaccaaac
     1501 tagatttctt tcaattttat ggctcttttg gaactcgtct aaaagctgaa tcgttacctg
     1561 taataacgct ttggctgctg tcgaataata cacgctttct gtttctgcta gattggttaa
     1621 tttatctcga atggcaatat cattgccatt ttttacagga ttataacgca aatcgccacc
     1681 gtccacaaag acttcaatcg ggcgattgta tgcctctgcc acttctttta acgcttgtaa
     1741 agtctctcct gctcctttcc catcgactaa gataacgggg atacgaaact tacacgcatg
     1801 ttctgctagt aattggatca ccgtcgtttt tcctgaacct gtagtcgctg gaataatcac
     1861 atgttggttc aattcactaa agttcatcac ataagggagc ccatactcat ctgttcctaa
     1921 tagaatcact ttggctaatg aagccagttg tttattccgc aaagcaacgt cttttatgac
     1981 aattgctacc tggtacgctt gctgatactc tgctgtatat ttttgggact cctgaaattt
     2041 attcttccga cgttccttgt attctttact atttagcaaa gctgtttgcc gttctttctt
     2101 tgaaacaact tttctttttt caataaaaga cgcatacgca atcaaacaac ttcctgcacc
     2161 tgtcccacct aaaaggaggg caaaaatcgt ttgtccatta aactgcaacg catgagaaaa
     2221 caagtttaat ggaaataata agtaacacga caaataccct ctaggaagaa caaaaataaa
     2281 acaaaataga tttaatagcg taaaacctaa cgcaaaaata aaaaaaagag agcttcttcg
     2341 ttttaagaag ctctcatact caaagtcata aatatgtgca cgtgtctctt ttttatctat
     2401 cataaagaca atcgctaaag gaatactgcc tacaataaaa aatggatata gacaaacacc
     2461 taataaagtg acgataaaat aaagttgttg aaaccgactt tttggcgttt ctttttcttg
     2521 ttttcttttg cgatcgacag aaaacatttg ctcaaaatgt gtcggtcgtt ttggtgcatt
     2581 catcatacta tcgatttcct cctatcgttt tcgttgtttc atttctcttc gataaatcgg
     2641 ttgtttttct cgttttcgtt tcatttttgc ttgagttgct cgaaactcac tccgtgtctt
     2701 cggtgcttgt tttggtgatt ttgtttgttt catggttgag tatgctttag atacggttga
     2761 aggcgctttg gtttgtgctg ttactcgatt gactgctgac atatgttccc gaaccccctc
     2821 tggacgtcga acagatttcg gtgtggtaga acctgttgct cgtggtacat tcccttttct
     2881 tagtggtttt tgtaccgcat gtttttgtcc tgcaactgtc cgggttttca tcgaacttgt
     2941 ttttgttacg ggagtaagtg cctccttccg acgacgactt gtttgtccct gacgacgttt
     3001 tcgatcgttc aattctgttc taggcgtatg cttctggcca taagacttgt gtttttgctt
     3061 tggattcgct aatgacacca ccattgcaga accctgattg cgatacggac tacgagacgc
     3121 accttttcta cttctttctc ttagactagg attgccttgc aattctcgtt gtaataaatc
     3181 ggctgttttt cttgcctgtt tctccattgc tgttcgttgc atacgttctt gactttgctc
     3241 ttctgcttgt ttcgcttgat catgcaagtc tcttgctttt tgtgccatac gttccatacg
     3301 atttggtgct ttatgacgag gttgttgtgc ggattcacgc cgaaaagcac ttcgtgtggc
     3361 ttgtttcgcc gtttgtggca tacgaacgtc tgagccaccc ccacgttttc gttgacgttc
     3421 ttgtctcttc cgtgcgtttt ctttttcttt ttgtttctgt gcttttctcg cttctttgcg
     3481 ttctttcaac cgttgtttgg cttcccttct caattttgct tctgccccaa atggatttcc
     3541 cgcaaaagct aacgctcctc gaacacccat tcttccagta aatagactgc ctaaaaagcc
     3601 tcggaacaaa taaattccca acggcgcaaa caaataaaca atgatcaaga gaattttttc
     3661 ccatggatcg tcttgtgtac tacttaattg gaacccgtaa ctaagaaaag aagaaaaaag
     3721 gatacatgca aaacctgtca ttccttttaa aaacgtcaaa ataccaaaaa ctttggcata
     3781 gttaccaaac acattcattg tgacaagaaa caatcctaaa aaaagtaaga acggcaacat
     3841 cggtactaac aatagttgca tagttccaat aatcaatcga aacatactta agacaaaata
     3901 aacaacggtt tgaatcagat tcacgacaaa ataaaacaag gcaaatgctg tatttttcca
     3961 actattgtaa tattgtatgt tcttgttttt cagtgtatct gcttcatact ctgtgacttt
     4021 gtcatgaatt ttctccccta tttttgtacc taaatcattg tccaataaaa gattgattcg
     4081 atcatattct tttccagcat atttgacttt cttctcacga attgtgtcta cattggttgt
     4141 gccataattt aacaacagat atggttcata aacattacta taaaagaccc gagccgccat
     4201 caatttgcct gccgtttcca cttttttatc cgctgttacc tcttctgttt cttttgtttc
     4261 ataatttgtt tcttcttcag gtgttaacac tggattgacc ttcacgaacg ccgtttccac
     4321 tgtagaatct aaagaaaaaa gaacatcaaa aagattctta tttgtcccag aatcttttaa
     4381 tacgcttaac ccagtaaaaa taaggatcgt cataagaaag acacggagtg cttgtttcat
     4441 tcgagcctct ccagcatatt tgattgccat cataattgcc gaaaaactga ttccaatagt
     4501 tcctgcaata cttaacatat tgtccgcaat tttttgtgtt aagttttgaa tgggttctcg
     4561 aatggcggaa ataatatcta attcaaagag aaattcaacc attgaagcat tgaattctcc
     4621 taacattttt acgccactcc aaacaaaatt tttcatcact cctgcaacat tgtacgatag
     4681 ctttttttta aggttcattg actcatagtc attggtcatc aattcaaacg aattcttttt
     4741 gtaattttta tacaattgat ccacatcagt cgttacttct tttacttcta ctttatcgtt
     4801 ggcattctct ccttcagaac gatcggtttc agtgacaccc gcaccatcat tcatttgatc
     4861 ggcagaaaca gtagcccctt gtagacagaa aaaaagaccc cctaataaaa ggagcgtcca
     4921 ccatttcaga gatttttttc gcaaaaaaac cacctctttt ttgttttatc gttgtttttc
     4981 attttctaac attgcttgaa ttttttcatc ttgattttca gtagaagaaa cagccaataa
     5041 ccaactatca aacaacacat caatggcaat cggttgattt cttcccaaat gatcctggaa
     5101 taaacaggtt cctgtttgta attgttccat catttctaaa ttggctgaat tcacttccat
     5161 atcaaagaag gacaatagtt tctctcgtgc ttctttttgt ttcggacgaa acgcaaattt
     5221 gtaagaaatt aactcttttt tatcttcttg atcgtaatct ttaaacgctt gcacaacaag
     5281 gtaaatatcc gtattattcg cacgtccttt tcgcaagcta tcatcaatca aatagcttcc
     5341 ttgtgcggta tcttcatacc cttttgcctc gtcataaata atcgcagcgt cttcttcttt
     5401 gtttgtagaa aagatatacg taaacttcat aatgacttcc ataattgcca ttcctgcaag
     5461 ttgttcgcta tttaatcgac ccgaatcaat ctctttttgt gtcggaatgc gtattccttg
     5521 tgtccctaac acgttgattt gtgaatcaaa acgtatcggc gtactgtgct cattcccaat
     5581 gagaatcttt cctaaaccaa cgctatgccc taaaatcaat ttagctagtt ctttgtcact
     5641 ttttcgaatt tcttcgataa cttgacttaa cgaatgctta cctggcatgg tgtaaactac
     5701 atcgatggcg tcttggatca ccgttttctt gtggctcgct gtttctcgat tggtatctac
     5761 ttctcctaac attcctaaca catttttagc ggtttgaatt gcctcttctt ttggtaaaaa
     5821 caatagagga tcaagcatcc ctcgacagct ttcatctgaa gataacgaaa taaaattaat
     5881 tcgttcatac atagccacaa actctggtat gtgtccgaat ttttcaagtg cctttaaaaa
     5941 gaacttttgg gtttcatttt tcggatcaat ataaagaatt ttttgaccta aaaacatcag
     6001 ccataaaaaa atatatttta caaggaccga ttttccttta cctggtggac caataatcaa
     6061 cgtatttcca ttggtatgtt ccgcgccttc aatacttttc tttgttaaat tgggataata
     6121 ccatactagt ttactagata gcgctaacgc ttctttcgca gatttaaact tttttgcaat
     6181 aacaatccgt cctaaaggaa atcctttctt atttcctact tctttcttta agtccaagcc
     6241 ataatctgcc acaaaacctg tcgtagcgac ctgctcgtaa ctacgaaaac gttgcgtact
     6301 tccaattaag cattgattaa acaacgttag ctgatccgct accgatcgat aaaatttgaa
     6361 atctgtagac tcaatcgcaa attcaatatc tttgactcgt tcttccaatt cttcttttga
     6421 agaagcagat acaaccagcg tgacggtaga ataacacaat cgtttttctt tgcgcttcag
     6481 cttatctttt acatccatta accgttctgt accaaaattt aatacatcgt ctgaatctaa
     6541 aatgccgtca acggtctcat attctttttc ttgttcataa atccgtccag ccattttatt
     6601 aatttttcgt tcatcagatt ttgaatgctc aaatcgaagt cgaatatgcg tatcaattgg
     6661 aaaagataag ctatcctgaa gattttgaat aaagctactc ccatacatgg ctaaaggagc
     6721 gtccgtttga cttaaaaacg tcgaataatg cgtttgattc aactgttcaa tcgcaatgta
     6781 tcctgcttca ttcgaaatga cgccctcgca aatttccatt ggcgtaagct gtctttcagg
     6841 caatttttct tctgatcgat ggaaaaaata ataataaatt tgtccaatct cttgggcaga
     6901 taagcgctct atcggcttat aattggacaa ttccgtatag agctcctttt ctaatgtttc
     6961 atacgttggt aacacactac ttataggaac tcgttgtccc gtaatacgat agataaactt
     7021 tttaaaaaca tgcttaagca attgaaaata ttcgatcgga tcagacactt tcatttctgt
     7081 agtgaaacgc acaaacagat acgttcgata ctcgtttaat tgcacttcgt cttttaaaat
     7141 ctttcctgcc tgttcaaagt acactttccc aagatctgcg cattctccct ttaccaaatc
     7201 tcgaatggtt tcttcaatgt gcgtatctag gttaaattga cgtggaatat tgataaaatg
     7261 atagtcatat tcatcatgtc caagtatgcc ttcgccatct tgaagatagt tcttgaaaaa
     7321 atcaatgtca tttaaaggaa atacttggga agaaagttga tagcctaccc acacatcatc
     7381 atttttggtc aaaacaaaat ttcggataat ggcgtctatc ggaaattcta atcgaggaag
     7441 cttcgttttt ttctttttga actgccaaat cccgataata aagacgacca aaagaaacaa
     7501 aaacgccatt tactcgactc actccttcca tttgtgtagg gaatacgtca ctttttcatg
     7561 gaaaaacggc acatacattt catgttcaaa gcgttgtttc cgtttctgat aataggacgt
     7621 tcgcccaatt aaatactcga aaaagtgttt attgtcccat ttcattgaaa acaatgtcca
     7681 aacgagaaca ccaatcccaa aaatcataat caaccaggac tttcgaaaca gtgtctgcaa
     7741 gaaactaatt tgattcacat acgcaatcaa aaataggatc aaaagaatga agagaaatac
     7801 cccacctatg actaattgtt ccatgcgaat gccattagaa atccagggaa ttttttgtcc
     7861 tttaatcccg taaattttgt gagggtgctt aaactccttt gtatagttgt attccatgtt
     7921 cactctacct ccttttacaa aaaaagaagc gaccagaagt tctaatcttc tgatcgcttt
     7981 tgcagtttgt ctttttctta tgataattgt atccagggca caatcatttg cgttaccccc
     8041 acgttctctt cactatcctt aataaagata atgttcttta gccattcacc aatatcaacg
     8101 gcattcgata acaataagac gagcattatc aaaataaaga cgccaataat ggcaaacgct
     8161 gcccgcgcaa agccttgggt cgcataagca ccaaccatta gaacaatcgc aacgattgcc
     8221 aatagccaaa tgacttgtcc agatacatca tcaaataacg atttaagatc aaaaagtttc
     8281 atttccattc tcctctaagt tcattttatt catttaacgt agtaacaaaa taactatcgc
     8341 cacttgtttt tacttccatt gaaaagtttg attggaactc gtttcctttc tcaaaataga
     8401 aacgatactt cccttgcaaa aagtagtttc cttctttatt ttttactgca tgatccagtt
     8461 gtacattgat caattcgccg ccttctaacc ctttttcgtt agaaatcaat gccaattttt
     8521 catcattttt gacatataac tcaaaaaacc gtttcacaaa tgcttccagc ttgatttctt
     8581 cgctttcagt gaccgcttgc ccagaagaat aaaatcgatt ttcctgatag ttcacagaat
     8641 tggtattctt actctcagaa agattcaaaa attgaacgcc atcaagtact tttaaaacat
     8701 tgttttgata actgactgga aaagaaagtt gactgctttt ccaatgttct ccttctgtaa
     8761 aagtaacctc ataaaatagg cgatacattg cttgtgtcaa atcgatttct tctagtttaa
     8821 taaagcgaac atctttcgct actcgattgg tagccgtcgt tacaaagcct agatcagacg
     8881 cttccaatcc ttgtgctaaa taatgattga cggaagcctc ccattcgttt cgtttttctg
     8941 gcgaataagt aaataacctt tcaataaaat gttgtccctc gtacttcaat tgttccgtag
     9001 aagacaaggt ttggttgact tcagttaaca atgtttctgg attgacttgt tttttcactg
     9061 ctaaacgcgt taatgtatcc actcgtccaa agtttaggag gagcacgaca cccattaaca
     9121 gaaacaaaca aaaaaaagcg cctgaaaata cactcatggc acttttttgc gaaaattgga
     9181 tcattcgatc tgacggttct gttttctttt tttggaacaa aaaacgtttc ttttgtttct
     9241 tttccttctg ttgtttcggt ggcttagttt ggaacgcttg tggattaaat tttgcattac
     9301 tcatgagaaa tgccataccg ttctaacaat aatttcggta ttttgtccac aatcactact
     9361 tcgtggacaa ccacacttgc cccctc
//
LOCUS       DAZAOG010000108         9281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000108 DAZAOG010000000
VERSION     DAZAOG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.108"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHDLD_002067"
     CDS             <1..435
                     /locus_tag="ACHDLD_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN3363753.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHDLD_002068"
     tRNA            complement(759..842)
                     /locus_tag="ACHDLD_002068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHDLD_002069"
     CDS             1370..1870
                     /locus_tag="ACHDLD_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363754.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHDLD_002070"
     CDS             complement(2060..2404)
                     /locus_tag="ACHDLD_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN3363755.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHDLD_002071"
     CDS             complement(2558..3478)
                     /locus_tag="ACHDLD_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3363756.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHDLD_002072"
     CDS             3665..4156
                     /locus_tag="ACHDLD_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN3363757.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHDLD_002073"
     CDS             complement(4180..6555)
                     /locus_tag="ACHDLD_002073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN3363758.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHDLD_002074"
     CDS             6881..7507
                     /locus_tag="ACHDLD_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3363759.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHDLD_002075"
     CDS             7530..8234
                     /locus_tag="ACHDLD_002075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3363760.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHDLD_002076"
     CDS             8426..9259
                     /locus_tag="ACHDLD_002076"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN3363761.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DAZAOG010000109         9107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000109 DAZAOG010000000
VERSION     DAZAOG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.109"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHDLD_002077"
     CDS             <1..1872
                     /locus_tag="ACHDLD_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN3363762.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="ACHDLD_002078"
     CDS             complement(3807..4472)
                     /locus_tag="ACHDLD_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363763.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4598..6634
                     /locus_tag="ACHDLD_002079"
     CDS             4598..6634
                     /locus_tag="ACHDLD_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN3363764.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="ACHDLD_002080"
     CDS             6638..7276
                     /locus_tag="ACHDLD_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363765.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="ACHDLD_002081"
     CDS             7304..7732
                     /locus_tag="ACHDLD_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363766.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..9100
                     /locus_tag="ACHDLD_002082"
     CDS             8189..9100
                     /locus_tag="ACHDLD_002082"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3363767.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         3163 a         1394 c         1721 g         2829 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtat
//
LOCUS       DAZAOG010000110         9060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000110 DAZAOG010000000
VERSION     DAZAOG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.110"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..283
                     /locus_tag="ACHDLD_002083"
     CDS             89..283
                     /locus_tag="ACHDLD_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363768.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            552..851
                     /locus_tag="ACHDLD_002084"
     CDS             552..851
                     /locus_tag="ACHDLD_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363769.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(948..1382)
                     /locus_tag="ACHDLD_002085"
     CDS             complement(948..1382)
                     /locus_tag="ACHDLD_002085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363770.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1614..2324)
                     /locus_tag="ACHDLD_002086"
     CDS             complement(1614..2324)
                     /locus_tag="ACHDLD_002086"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN3363771.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2314..3174)
                     /locus_tag="ACHDLD_002087"
     CDS             complement(2314..3174)
                     /locus_tag="ACHDLD_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN3363772.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3179..3817)
                     /locus_tag="ACHDLD_002088"
     CDS             complement(3179..3817)
                     /locus_tag="ACHDLD_002088"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN3363773.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3832..4131)
                     /locus_tag="ACHDLD_002089"
     CDS             complement(3832..4131)
                     /locus_tag="ACHDLD_002089"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3363774.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4174..5658)
                     /locus_tag="ACHDLD_002090"
     CDS             complement(4174..5658)
                     /locus_tag="ACHDLD_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN3363775.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5678..6139)
                     /locus_tag="ACHDLD_002091"
     CDS             complement(5678..6139)
                     /locus_tag="ACHDLD_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3363776.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHDLD_002092"
     CDS             complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHDLD_002092"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN3363777.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7442..8212
                     /locus_tag="ACHDLD_002093"
     CDS             7442..8212
                     /locus_tag="ACHDLD_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3363778.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8485..>9060)
                     /locus_tag="ACHDLD_002094"
     CDS             complement(8485..>9060)
                     /locus_tag="ACHDLD_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3363779.1"
                     /translation="SKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLDHLYAKKPDA
                     ALFYFNQVLEQTTETDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNL
                     MVCIVYYDIATYYRKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGE
                     DDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2827 a         1863 c         1438 g         2932 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      481 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      541 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      601 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      661 ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata tacgagatga
      721 aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa aatcatgtgt
      781 tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag gtgagtaaaa
      841 aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat cctcttgttt
      901 ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta tttgtcatta
      961 ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc catttccttt
     1021 acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc atctgtcaat
     1081 aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt caaaagcact
     1141 ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat aaatgcgatc
     1201 gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc attacgcttt
     1261 atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt tgtgtaattc
     1321 gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga atcaatattc
     1381 atttttttct tcctttctta tacaattctc cctctaataa tacagttctt tgactattta
     1441 atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt cctctttcct
     1501 tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt cacaacctct
     1561 gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca tctctattgc
     1621 ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa atattgcggg
     1681 atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca aatttcatct
     1741 aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa cggtccatgt
     1801 ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt aaacgtctcg
     1861 acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt cgttaattgg
     1921 cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata agctggcaaa
     1981 tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt aatagcagtt
     2041 atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga tggttttaag
     2101 ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc tgcttgcatg
     2161 cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg gtccacttca
     2221 ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag atttgcttca
     2281 aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc cagcctcctt
     2341 caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt cttggctttt
     2401 ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca aatgtcttaa
     2461 taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact ggcctgaata
     2521 ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat cgatcccttt
     2581 ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta ggtctggata
     2641 tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg aaatggagtt
     2701 cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag aagcttcctt
     2761 tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt tctcttcata
     2821 ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg ccaaatggat
     2881 tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag aaccaaacgg
     2941 aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc tgaacatctc
     3001 ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc gctcatccgt
     3061 ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg aaaaacgttc
     3121 tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag ccatttcctt
     3181 acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc ggatcatttg
     3241 ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca aataagtgta
     3301 atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca atcagttttt
     3361 tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca tcacggcttt
     3421 gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt tcaaaagtcc
     3481 aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc tccatagttg
     3541 gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca acattactcg
     3601 ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta tctgggaaca
     3661 tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt gtgcccactt
     3721 caataatatc tactacatca ccaactttca taacatctcc taatgcatgt tctaagtcat
     3781 tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc tttacttgtt
     3841 tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg acatcacatt
     3901 tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat tcaaaaaatt
     3961 gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg aaatggcaca
     4021 atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca cacttctcat
     4081 catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca tcttaatccc
     4141 tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt ctgcatatgt
     4201 gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt gtaactgcgg
     4261 aatagccaat aaaccaatga caactacggc aattccagca taacctagga ctttcatgat
     4321 tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac caccgtatgt
     4381 tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga ttcctgaaag
     4441 aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa ctgctataac
     4501 ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg atttcaataa
     4561 aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc caatcgttac
     4621 cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa cagctcctgg
     4681 taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga atgtccgaac
     4741 acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca taatgtagaa
     4801 taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt tgcctagtac
     4861 aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca tattctcatt
     4921 gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt gcttgattga
     4981 tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa atataaagat
     5041 tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt cctgcgtgat
     5101 tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc ccataaaaat
     5161 caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa aagcggtagc
     5221 tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg tatatttctg
     5281 aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca tcacatctcc
     5341 aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc caaaataagg
     5401 atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga ttggccgaaa
     5461 attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg ttgcctttaa
     5521 gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta ataacacgat
     5581 aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg tccaaatgct
     5641 taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct gccatcacaa
     5701 cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca tctgtcatta
     5761 ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt gctgcgagtg
     5821 taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa cggattggct
     5881 gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt tctgaagaat
     5941 gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct acacaatgga
     6001 tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca agacagcttt
     6061 cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc aattgatggt
     6121 caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg gaaacggtgt
     6181 atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact caagatcatc
     6241 tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt taaatccata
     6301 cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat cagccataaa
     6361 attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg actccgccat
     6421 gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt caccataggc
     6481 ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat tggagtaatg
     6541 tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt ttactgcaat
     6601 ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt cacaagtgac
     6661 caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga ttttgactgc
     6721 atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt gccacgtgcg
     6781 aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa ctgctgcggc
     6841 tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt ctacattttt
     6901 tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca tattttgaac
     6961 gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat gactacgttt
     7021 tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt tcagccaaat
     7081 tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt ctgtttcgat
     7141 ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact tttcttttgt
     7201 tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt ccaacttaca
     7261 agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg tttgtttttt
     7321 atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag atatgaatgt
     7381 ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg aggcaaaagt
     7441 catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg aactgcttca
     7501 gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat ctattagtac
     7561 gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca attacggtta
     7621 ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg aaaggattaa
     7681 acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc tgtttatcaa
     7741 ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt tgacagtcat
     7801 tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt atattttaac
     7861 tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat taaatacgat
     7921 ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg tagtacatgg
     7981 ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca acaagacaat
     8041 caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa agttcaagat
     8101 tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg aaaaagtatt
     8161 agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt aagaaccagg
     8221 acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg ataaatgaaa
     8281 aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa tcgataccat
     8341 cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat caaacaatat
     8401 cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg gtattcaaat
     8461 cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct ttaccgcgta
     8521 atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta aataatcatc
     8581 ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca atgcataagt
     8641 cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg ctttatcata
     8701 atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga ccatcagatt
     8761 atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt tcgcaaaatc
     8821 taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta agtgatggat
     8881 atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg catctggttt
     8941 tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta aaagatcaga
     9001 attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt ggtattttga
//
LOCUS       DAZAOG010000111         8716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000111 DAZAOG010000000
VERSION     DAZAOG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.111"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1127
                     /locus_tag="ACHDLD_002095"
     CDS             66..1127
                     /locus_tag="ACHDLD_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN3363780.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1130..2026
                     /gene="murQ"
                     /locus_tag="ACHDLD_002096"
     CDS             1130..2026
                     /gene="murQ"
                     /locus_tag="ACHDLD_002096"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN3363781.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2040..4022
                     /locus_tag="ACHDLD_002097"
     CDS             2040..4022
                     /locus_tag="ACHDLD_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN3363782.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4087..4533)
                     /gene="nrdI"
                     /locus_tag="ACHDLD_002098"
     CDS             complement(4087..4533)
                     /gene="nrdI"
                     /locus_tag="ACHDLD_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN3363783.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4744..6168
                     /locus_tag="ACHDLD_002099"
     CDS             4744..6168
                     /locus_tag="ACHDLD_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN3363784.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6349..7122)
                     /locus_tag="ACHDLD_002100"
     CDS             complement(6349..7122)
                     /locus_tag="ACHDLD_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3363785.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7119..8024)
                     /locus_tag="ACHDLD_002101"
     CDS             complement(7119..8024)
                     /locus_tag="ACHDLD_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363786.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8178..8498)
                     /locus_tag="ACHDLD_002102"
     CDS             complement(8178..8498)
                     /locus_tag="ACHDLD_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN3363787.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2728 a         1572 c         1865 g         2551 t
ORIGIN      
        1 tttgtttctt tagatatata ctctagtcat agaaatgaag acggatacag aaaaagaggg
       61 ataatatgtt tggcttttca atatttatga acgattcttt gtcagaagag aagaaacagt
      121 atatccgcca aatggcatcc aatggctttg tcggtatttt cacttccatg catataccag
      181 aagatgatgt ttcggcatat caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga
      241 ataaactaga attgatggtc gatatttcag gagaggcatt ggaacgagcg ggtttttcat
      301 tagaagatct aacgcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata
      361 tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca
      421 cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg
      481 aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga
      541 aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata
      601 cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc
      661 atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag
      721 atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag
      781 tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta gggaagcata
      841 cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga
      901 ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata
      961 acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg
     1021 atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa
     1081 tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat
     1141 caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat
     1201 gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt
     1261 tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa
     1321 aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc
     1381 tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg
     1441 tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca
     1501 agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag
     1561 cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac
     1621 aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga
     1681 agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca
     1741 aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa
     1801 taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat
     1861 catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga
     1921 tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca
     1981 agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca
     2041 tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg
     2101 gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca
     2161 atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag
     2221 aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa
     2281 tggttgattt ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag
     2341 caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg
     2401 cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata
     2461 tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag
     2521 ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg
     2581 atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca
     2641 atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga
     2701 tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg
     2761 cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg
     2821 agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt
     2881 cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt
     2941 ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct
     3001 tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc
     3061 caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga
     3121 tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata
     3181 aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac
     3241 cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg
     3301 gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac
     3361 cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg
     3421 gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag
     3481 accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa
     3541 aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa
     3601 cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag
     3661 gctactttgt agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct
     3721 ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc
     3781 ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag
     3841 aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag
     3901 cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact
     3961 ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt
     4021 aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct
     4081 tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt
     4141 tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc
     4201 tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag
     4261 tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc
     4321 accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc
     4381 tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac
     4441 aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt
     4501 atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta
     4561 gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc
     4621 ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag
     4681 taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct
     4741 tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta
     4801 attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct
     4861 ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg
     4921 tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca
     4981 gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc
     5041 tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca
     5101 gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga
     5161 tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca
     5221 ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc
     5281 caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc
     5341 ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt
     5401 gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg
     5461 attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg
     5521 acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt
     5581 gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta
     5641 gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa
     5701 gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct
     5761 atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt
     5821 gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta
     5881 ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg
     5941 cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg
     6001 cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa
     6061 aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc
     6121 ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga
     6181 agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt
     6241 gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc
     6301 ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt
     6361 tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa
     6421 tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg
     6481 gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag
     6541 tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat
     6601 ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca
     6661 taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt
     6721 agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac
     6781 aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac
     6841 taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc
     6901 ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc
     6961 actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag
     7021 aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt
     7081 cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt
     7141 actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct
     7201 gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt
     7261 tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc
     7321 gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc
     7381 aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg
     7441 accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta
     7501 gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa accgttcatc
     7561 ggctcatcta aaataacgat agacggatgg ttcagtaagg caagagcaat tcccaatttt
     7621 tgtttcattc ccaacgagta ggatttcgct tttttgtgaa tgtacttttc catcttgagc
     7681 tgattcacga tagtgtcgat agcagataca tcgttggttt cagagaataa cttcagatga
     7741 tcatagcctg ttaagaatgg atagataccc gggtattcga tcaatgctcc tacttgttcc
     7801 aaccctttat gactagaggg agaaatctca ttttcaccaa tacagattgt tccatctgag
     7861 taatggatca gtcctaaaat agctttcatg atcgttgttt ttccagcgcc attaggacct
     7921 actaatccaa cgattttgcc aggggataag ctaaaactga catcgctgag ggcttgtttg
     7981 tgaccaaagt tcttggataa ttcttggacc gaaagacttt tcaaaaatat tcactccttt
     8041 tctatagata gtacaagcat acacctgatg agtggagaag tcgctaggtc attggactta
     8101 tttcttattt tttcataaaa aaattgaata ttaaataaaa aggcttattt tttctgatca
     8161 gtacttattc caagtgtcta gtggccgcta gtaatcttga tgccaatgat actgatgagt
     8221 agcatcccca caaagaacca agtggaaaga ttcagcttat catgaaacaa gaccacacca
     8281 attatgaccg aaccaactgc gccaatccct gtccagatag gataagagag actcatcggc
     8341 aaacttttga ttgcctttga caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa
     8401 aaagaataat tgattttaga aaaaccatca ctccatttca tcatcgttga ccagaatact
     8461 tccaaaattc ctgctatgat taattccatc catgccatac ttacattcct ccttaactat
     8521 aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct
     8581 ctttatccta cccggtggca gattgtatat ctttttctgt agtatagcac acctatccag
     8641 atatcggcaa tctcatactt tttagtaaag tgtaccaagc actgacggat atatcgttag
     8701 cggaaaagaa gatgaa
//
LOCUS       DAZAOG010000112         8675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000112 DAZAOG010000000
VERSION     DAZAOG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.112"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHDLD_002103"
     CDS             <1..1101
                     /locus_tag="ACHDLD_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3363788.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHDLD_002104"
     CDS             complement(1280..1615)
                     /locus_tag="ACHDLD_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3363789.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHDLD_002105"
     CDS             complement(1599..1862)
                     /locus_tag="ACHDLD_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN3363790.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHDLD_002106"
     CDS             complement(2322..3125)
                     /locus_tag="ACHDLD_002106"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3363791.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHDLD_002107"
     CDS             complement(3103..4227)
                     /locus_tag="ACHDLD_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363792.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHDLD_002108"
     CDS             complement(4243..5058)
                     /locus_tag="ACHDLD_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3363793.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHDLD_002109"
     CDS             complement(5048..5920)
                     /locus_tag="ACHDLD_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3363794.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHDLD_002110"
     CDS             complement(6005..7294)
                     /locus_tag="ACHDLD_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3363795.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7971)
                     /locus_tag="ACHDLD_002111"
     CDS             complement(7438..7971)
                     /locus_tag="ACHDLD_002111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363796.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHDLD_002112"
     CDS             complement(8017..8160)
                     /locus_tag="ACHDLD_002112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN3363797.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZAOG010000113         8640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000113 DAZAOG010000000
VERSION     DAZAOG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.113"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..397
                     /locus_tag="ACHDLD_002113"
     CDS             <1..397
                     /locus_tag="ACHDLD_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3363798.1"
                     /translation="DFYNLKKFNHPLVFESDSSENHYNGKYYHQHHQVHEFKSILLTS
                     DQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQDFIVAMQEVNEQVNFQYTTRQK
                     DQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            427..549
                     /locus_tag="ACHDLD_002114"
     CDS             427..549
                     /locus_tag="ACHDLD_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN3363799.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            567..2090
                     /locus_tag="ACHDLD_002115"
     CDS             567..2090
                     /locus_tag="ACHDLD_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN3363800.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            2114..2353
                     /locus_tag="ACHDLD_002116"
     CDS             2114..2353
                     /locus_tag="ACHDLD_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363801.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2546..3007)
                     /locus_tag="ACHDLD_002117"
     CDS             complement(2546..3007)
                     /locus_tag="ACHDLD_002117"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3363802.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3202..3420)
                     /locus_tag="ACHDLD_002118"
     CDS             complement(3202..3420)
                     /locus_tag="ACHDLD_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363803.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3538..5154
                     /locus_tag="ACHDLD_002119"
     CDS             3538..5154
                     /locus_tag="ACHDLD_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3363804.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(5395..6735)
                     /locus_tag="ACHDLD_002120"
                     /pseudo
     CDS             complement(5395..6735)
                     /locus_tag="ACHDLD_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7173..7319)
                     /locus_tag="ACHDLD_002121"
     CDS             complement(7173..7319)
                     /locus_tag="ACHDLD_002121"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363805.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(7358..7552)
                     /locus_tag="ACHDLD_002122"
     CDS             complement(7358..7552)
                     /locus_tag="ACHDLD_002122"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3363806.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(7462..7794)
                     /locus_tag="ACHDLD_002123"
     CDS             complement(7462..7794)
                     /locus_tag="ACHDLD_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3363807.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(8308..8499)
                     /locus_tag="ACHDLD_002124"
     CDS             complement(8308..8499)
                     /locus_tag="ACHDLD_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363808.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         2805 a         1390 c         1677 g         2768 t
ORIGIN      
        1 ggatttttat aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attcatcaga
       61 aaatcactat aatggcaaat actatcatca acaccatcaa gtacatgagt ttaaatcaat
      121 tctcttaact tcagatcaag acataaaaaa agatttttta ctccaatgga ttgactggct
      181 gattttaacc aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tacaagagca
      241 ggattttata gttgccatgc aagaggtaaa cgaacaggtt aattttcaat acaccactcg
      301 acaaaaagac caaaccacta ttattctaat cggaaaaatg ttggatgaag aaaggatcaa
      361 acagagtttt gataaattac tggcggaaag caaataatga taagtagtga ttgggggtaa
      421 tttctaatgg agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatt
      481 aaaactagaa aaagggcatt aaaaattatc aaagaacata aacgaaataa aatgaagaaa
      541 ttagcataat taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag
      601 cagctctagg attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata
      661 aagatggaaa attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca
      721 tcgtcggcga tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact
      781 atgagccctc agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata
      841 acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta
      901 atgtagtaga aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg
      961 atcacggaga agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa
     1021 tggcaattaa ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag
     1081 caaaagtatt tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg
     1141 actataccac tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct
     1201 ttggatattt agctttggat tatggattga aacaagttcc tattgctgga ctaagtcctg
     1261 aagaagagcc atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga
     1321 tcaattatat ttactttgaa aagaatgcta atgataaaat tgcaaaaact ttggccaacg
     1381 aagctgggat aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg
     1441 ataatggtga agattatgtt tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa
     1501 ctatggttgc tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg
     1561 ctaatgggta ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg
     1621 aatggcaatc tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca
     1681 aagcaaaaat caataaagac atgacagctg ccgagtacaa agattattat actactggct
     1741 ataagacaga tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg
     1801 gtgaacatca tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag
     1861 gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat
     1921 atattcaatt tagtgatcat ggaattgcac caagtaaggc agagcatttc catattttct
     1981 ttggtggaga aagtcaagaa aaactctata atgaattgca taattggcct actttctatc
     2041 cagcttcatt gtctaaacat gaaattgctc aagaaatgat ggctcattaa aattcaattt
     2101 ttggaggaaa tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc
     2161 ttatcggcgt tatcgatttg caaagcaaat gagattaaat gggagtacat tagctgatat
     2221 tgctgcaact ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga
     2281 ttgttgtttt atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag
     2341 cttggccaat taattgataa taccagagta gatcattttt cccttatttg gatttgatct
     2401 gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca
     2461 taaggtgttc tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga
     2521 tttatcgttg agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg
     2581 agtctattcg tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa
     2641 agggcatatt attcaatgct tctgtaactg cgaaatgatt ggagaattct tttcctctat
     2701 ctggagtgat cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct
     2761 cgctcacata agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt
     2821 ttctgtctgt caaagtcact aaacaagtac caccagtttt gccagctaca atatcggctt
     2881 tccaatgacc aaaatcactt ctatcatctg cggattttgg acggccatgg atcgaatgag
     2941 taaggcaaat ctttccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat
     3001 ggtgcaatga acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat
     3061 cattgcaaat tgcacgttag atcgtgataa aactaattga aaagtccata tgttcatgct
     3121 tcaaacgatt cgagatttcc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa
     3181 ttgaccaaac tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag
     3241 cataattttt ttccgctttg gaaggtgaat agtttttttc ttgtttcttc tcagttcatg
     3301 taaaatagta aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc
     3361 ttcgtgaagc agaaagattc tttctcgttc gagtatagta agatgacgat actggctcat
     3421 aattattttc cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga
     3481 ctcattttta tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg
     3541 aaaaaaatac ctattacaat cttaactggc tatcttggtt caggaaaaac gatacttcta
     3601 aatcatattc tgtacggtga tcacggaaag gaaattgctg tcattgtaaa tgacataggg
     3661 gaagtaaatg ttgatggggt attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa
     3721 cttgtttcga tgccgaatgg atgcatttgt tgtacattac gcgaagattt gattgttgaa
     3781 ctagatcaat tagtgcaatt ttctgacttg aatcaaattg ttattgaagc ttcaggaatc
     3841 agcaaaccaa ttccaattat tcaagcaatt atattagcta aaaacccttt agacattgat
     3901 ttgacccaac gattagagat tggtgccatt attatagtag tggatgtaaa acgtttttgg
     3961 gataatttcg aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa
     4021 gaagaacagg atattcgtgg cttgttaatt gatcaattcg agtgttgtaa catattactt
     4081 ttaaataata gtgacttagt gtcaaccaag acgatttaca aaattaacgg tctaattgct
     4141 aagttacagt ccaatgccaa aataattagg acaattaaag gtgaagtaga cttatcgaaa
     4201 attctaaata caaatctttt taatatagat gagacttcct attcagtggg ctggttcaaa
     4261 gagttagagc ttggctatca aaatcatatt cctgaaacag aagaatatgg tatctcaagt
     4321 tttatctatc gaaatcggtt actccttgac gcacaaaaat ttgcccatct tacggataaa
     4381 aattttcctg gcaatattac aagagcaaaa gggtttcttt ggtttgccca acatagtgat
     4441 ttttcgacga tctttgaaac atcgggtagc tataaaagta ttgaatcatt aaattattgg
     4501 gtagctagtt tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag
     4561 gaagcctggc atccgttgtt tggtgaacgc attaataaac tagtttttat tggtatgaag
     4621 atggatcaac aatgtattaa ggaacaactt gagggttgtt taactgattc tcttaatgca
     4681 gataatttcc gaaaaatttc accatttcca tggaaaaagt ctaatatgtt tagccagata
     4741 tcgttaatga aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc
     4801 attttgatta ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa
     4861 acaactgaag atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc
     4921 ttcgatttgg ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat
     4981 aatattgttg agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa
     5041 atcgcggtta gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat
     5101 aagttagtta ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga
     5161 aatagatgag gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt
     5221 attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat
     5281 ctttaacggg acctgcaatg gtttagttaa cagtcggtta gattacttat tttggctctt
     5341 cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac
     5401 agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag tattcttagt
     5461 tttcttaaaa tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct
     5521 atagccatag gcaattcgtt ttagtacttt gatatggtta ttcaaacatt ctatagggcc
     5581 gttggaatag ggaacgcgca acgctcgttt gatttctgtt tggtatttct tgaaggtcgt
     5641 gaacactggt tggtagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa
     5701 ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc tggtagactt cgtagcctcg
     5761 ctttaaggaa tcatcataag ataatagccg atcgactatc tctgcttctg tcagatgatc
     5821 tcgaaatgac ggacgccagt agcgatgctg acctttcaat tcccacgcat tcttttgaag
     5881 tagtttccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat
     5941 tacttgaatt cggtggttgg taaaagcccg accaatgtgt tggctaatat gaaagcgatc
     6001 ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa
     6061 atctgaaacc actagacgca cttgttcacg gacttttata gggtagcgca agaaatagtc
     6121 gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc
     6181 catcatgaca aagctcatgg ctcccgagac atttttcact gatttgaatt catcgaaaca
     6241 caccacttct ggtaacagct ggctcaaaga aatggtttta ggccgaaggc tgcgaagtat
     6301 tcgaatgaca gtggtcggag aaatgtgctt catacgtgcg atcaaagaca tagaaagagg
     6361 ttcgcttaat agctcaagaa tggcttgttt gactctgcga gcaatggagc agcgacgatc
     6421 actaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt
     6481 gttaatccgc agaatggtct tgtagttact gacatcattc aataagatgg atactttttt
     6541 ccagccctat ttaatgatcg tttgttcatt ttgatagtgg cgatgaaagc attcctccgg
     6601 ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattgattc gctctttgcg
     6661 aaaggcatct tcatgaaaga taagatttag gtctagaata tctagtaact ctttggtaaa
     6721 ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag
     6781 gattttaagg tgtctgtttg cgtttattag aaaaaagaaa aagtattggc aacagaaggc
     6841 gtcaccaaca ctagatatta tagatccctt attttcttcg cttcaaattg tcaaggagtc
     6901 atcataaatt ttgtatggat tcttttgttt gggtagtagt cttctaaata atcttggatt
     6961 aacaccttca tctctatttt acttttatat ttgttggagt cgattaattc tcatttgatt
     7021 gatcgatgaa acgcttcaat tactgcattg tcaacaggag tcccagtttg gctcactgaa
     7081 tgtgtgatgt ggtggtcgtt caacagctct tgataaataa aagaccgatg ctggctacct
     7141 ctatcggaat gaattaccgt aggcaattct ccttaataaa tttgtaatgg acggacgaca
     7201 atctcttttt tcatgtgtga atctaccata tagtaacgta gaatacgagt tgctaagtca
     7261 atataggtgg acaaatagag gcgaccgtca ttacatggaa tgtaagtaat atccatcacc
     7321 caaacagcat caatttggtt ctgttcaaat tatcgattca acattttttt accatttctt
     7381 tgattgtttt ttctctacca tgagaagatt tgcgtcgatg aaatcctctt gtgcataaat
     7441 tcatttcacg cataagaaca gctacaacac gtttattgat ttcgattcct tcatcgtaaa
     7501 gtttttgagt gatccctgga gatccgtatc gatctttgtg ttcgtagaac actcgtcttc
     7561 ctcttttagt cgtttatttt cctgttcaag aatgtagaaa tctgctttat caggtaatct
     7621 ttttccttgt ccagcaaaag catttttccc gtatcgacga tattctgaaa cccatcggga
     7681 aattgttgga cgagagatat tgtattcttc agcaattttc ttggtagtta cttttttctc
     7741 aaggatatcc tgtgaaatct ttagtttaaa tttacgatca tatgtttttc tcaatagagt
     7801 tcattccttc cggtaacgta actgatagtc aagaaaagtg tatcatagcc caacttagta
     7861 tcagggtttg tcaactaaac tgtggaagtt gatagataag agtttttaac aaccaaaatt
     7921 ctcttgacta tttttgaaat cagacaaatt ttgattggac acatagttga ataaatctac
     7981 cgtcgttacg gaaggtaaat cgcatgcttt tcaattctag aggagaagtc ctcgttgaag
     8041 taccatagcc cagttctgga ttcgacctcc tgcccacttc atacgtaaat aaagtaattt
     8101 taggagtgag actgtgacaa aagttatttt taagctaaaa acgaattaaa aaacgttgat
     8161 aattaaaatc attcgtttta gattctatga atcattatag aacgctgtaa aatcgatact
     8221 ttataattat aaagaagata gtttcaaatt tattgttcga aatttgaaac tatcttttta
     8281 cttatgtcac agactccctt agttgagtta ttctttaatt tcgaatttcg agatagctag
     8341 atatattgca agcatacatt cttgataaca atcttgatca tattttccag ttctccagga
     8401 ttgcttatga atcaaagagc taaatctttt gagaattgcc tcaacagctt cttcatttcc
     8461 agcctttgct aaaatgaaat cttcttttaa agaattcata aatctacctt cttcccatgg
     8521 acttataatc tatttctttg ggaaggagga gaacgagcaa agaagcatac aaaattagtt
     8581 attatgcaga tattaaacca gcattttttg aatttttttg taaagtcgat gttttaaatt
//
LOCUS       DAZAOG010000114         8635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000114 DAZAOG010000000
VERSION     DAZAOG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.114"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..543
                     /locus_tag="ACHDLD_002125"
     CDS             64..543
                     /locus_tag="ACHDLD_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3363809.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            760..1851
                     /gene="phnW"
                     /locus_tag="ACHDLD_002126"
     CDS             760..1851
                     /gene="phnW"
                     /locus_tag="ACHDLD_002126"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFN3363810.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1844..1972
                     /locus_tag="ACHDLD_002127"
     CDS             1844..1972
                     /locus_tag="ACHDLD_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN3363811.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1976..2521
                     /locus_tag="ACHDLD_002128"
     CDS             1976..2521
                     /locus_tag="ACHDLD_002128"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFN3363812.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2975..4666)
                     /gene="argS"
                     /locus_tag="ACHDLD_002129"
     CDS             complement(2975..4666)
                     /gene="argS"
                     /locus_tag="ACHDLD_002129"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN3363813.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5086..6033)
                     /gene="arcC"
                     /locus_tag="ACHDLD_002130"
     CDS             complement(5086..6033)
                     /gene="arcC"
                     /locus_tag="ACHDLD_002130"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN3363814.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6148..7167)
                     /gene="argF"
                     /locus_tag="ACHDLD_002131"
     CDS             complement(6148..7167)
                     /gene="argF"
                     /locus_tag="ACHDLD_002131"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN3363815.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7258..8487)
                     /gene="arcA"
                     /locus_tag="ACHDLD_002132"
     CDS             complement(7258..8487)
                     /gene="arcA"
                     /locus_tag="ACHDLD_002132"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN3363816.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2477 a         1749 c         1592 g         2817 t
ORIGIN      
        1 attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta aaggagaaag
       61 aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga attcccgatc
      121 cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc cacgcaggca
      181 acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt
      241 tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga ggaagaggat
      301 cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca ctttttgacg
      361 atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga tgagatcaaa
      421 cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat ctcggaaaat
      481 gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt atcacttttt
      541 taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa tattttcctt
      601 attgaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa atttttcaac
      661 accttgattg aatcacaata atcatcattt atattggatg ttaacaagag cagcttcttg
      721 ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt attaacttct
      781 ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg
      841 gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga tcttgctcaa
      901 gtatcggaag gaatatacac aacggtctta atgcaaggta gcagctcctt cggggtagaa
      961 tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc aaacggcgca
     1021 tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac cgtttatgag
     1081 gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga agagaatccg
     1141 tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt gaataatttg
     1201 gaagagattg ctgctgtcac acaagaaaga gatcttttgt ttatagtgga cgcgatgtca
     1261 agctttggtg ggataccgat ttgtgtagcc gaactaggga ttgatttttt gatttccagc
     1321 tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa taaagcggaa
     1381 ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg atctatatga ccagtaccgc
     1441 gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac ggttttggct
     1501 tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg atattcacgt
     1561 tatctcgcca ataatcatgt gctacgacaa aaaatgacag agatggggtt ccagcctttc
     1621 attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga ctggcgattt
     1681 gattttaatc atttttatca gtatttaaag caaaatggat ttgtgatcta tcccgggaaa
     1741 atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc ggaagatatt
     1801 ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta aaactgtaat
     1861 ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc acgcttttag
     1921 aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgct aaccgatggg
     1981 aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca aaaaacaatg
     2041 gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt
     2101 tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg acacattgga
     2161 aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgaatg gatttaatgc
     2221 tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac cagacttcat
     2281 ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa
     2341 catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc
     2401 agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc
     2461 gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa agatcgttta
     2521 aaacagtctg ttgctcttag attccaacaa gccggcgctg atatttgtgt aaacaattta
     2581 aaagagttaa cagaatacat tttgtacgct gacatgaatc cagaatgact tttatacaaa
     2641 ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa gcaaaaatag
     2701 ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     2761 ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttgcaa
     2821 acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg
     2881 tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact
     2941 cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc
     3001 ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg
     3061 cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt
     3121 gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca
     3181 agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca
     3241 atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac
     3301 gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca
     3361 ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa
     3421 ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt
     3481 gttctttagc agattggatt gcttcattta atacttcttc taataagacg atctttcctt
     3541 tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt
     3601 cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc
     3661 tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca
     3721 atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg
     3781 ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca
     3841 aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac
     3901 cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg
     3961 attcatcacg gaaccattgc cataattgaa tagcctcttt atcgccttct tccagacgtt
     4021 tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt
     4081 ggacgtataa acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc
     4141 attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga
     4201 tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg
     4261 tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg
     4321 gtactgttcc ttgattcccg atgttgctat ccccataatg ttctttttct ttcactactg
     4381 tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga
     4441 caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct gcagcgattt
     4501 gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca acatctccgt
     4561 gttcagctga ttttggattt tctagtaatt gctcgacttg ttccagtgtt aaatcatctt
     4621 tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt attcctccta
     4681 aagtgttccc ttgattgtta aacaaaaaaa atctctagcg acggcatact tagtatgcca
     4741 tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag gaccagctcc
     4801 atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt
     4861 atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga tacaagtact
     4921 ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag aaaaaaaaac
     4981 gaaaggctag tcgtttcata aaaaaacaat ccagatggcc taaaggagaa gaaataccac
     5041 ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat cagctacgat
     5101 aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag tgatgattgc
     5161 cttagcgttt ggttttgctt caacaaatgc aatagctgct tctactttcg gaagcatgct
     5221 tcctggagcg aattgctttt catcaatgta ttgtttcatt tctgaaacag taacagtttc
     5281 taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc ctgtcaatac
     5341 gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat ctttatcgat
     5401 tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc caccgccacc
     5461 aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg cttctttaat
     5521 cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat cttctttgaa
     5581 tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt agaatggacc
     5641 gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa cttgagtgac
     5701 taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca tggcgttttg
     5761 taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag gcattgccgg
     5821 tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt gcggaccatt
     5881 tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat actttgctgt
     5941 ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga tggcattgcc
     6001 ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt cagttctacc
     6061 gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat gctgactcgt
     6121 tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt tacctaatgt
     6181 tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa attgacgagc
     6241 atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa agcgttcttt
     6301 catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt gcaagaagat
     6361 caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt aaggtttcaa
     6421 caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata catcagaata
     6481 aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca attgtgcacc
     6541 ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt gtaattcttt
     6601 tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata aagagttagc
     6661 catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg ttccaaagtt
     6721 ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat cagtcaagcc
     6781 attccaaact ggaacaccag agaattctgc tagttcctct accatttttt gactgaatcc
     6841 acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt ctgtggattc
     6901 ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac ctagatcgat
     6961 cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata aaagcgcaat
     7021 attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt tcaagtgttc
     7081 tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg ctaataagct
     7141 tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg tttttattta
     7201 agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg aaacaaatta
     7261 cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac gacctcgtga
     7321 caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca attcgttcga
     7381 tacataattt cggttataag tgactacaac acctggagcg attgccaatg tgtttgatcc
     7441 atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg ggatcagtac
     7501 aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg aatgagtaat
     7561 cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt ctttactttg
     7621 aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg tatccaaatg
     7681 catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc ctgaattttt
     7741 tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt gagaaatccc
     7801 aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat gatctggatt
     7861 ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat gtttcaaaat
     7921 atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat tgactgtcat
     7981 cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca ttgggtccat
     8041 gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac gtacttcgat
     8101 atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt ccattgatag
     8161 aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt catccaacat
     8221 tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg ctaatttttc
     8281 caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt catgttcttc
     8341 ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa tatctggtgt
     8401 caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt tccctatttc
     8461 agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg attacatgta
     8521 taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa tgagacattc
     8581 ctcacattaa tattcgataa ttaaataatt aaacgttttt ctatttttaa gcgta
//
LOCUS       DAZAOG010000115         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000115 DAZAOG010000000
VERSION     DAZAOG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.115"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHDLD_002133"
     CDS             complement(<1..149)
                     /locus_tag="ACHDLD_002133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363817.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHDLD_002134"
     CDS             complement(593..1714)
                     /locus_tag="ACHDLD_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFN3363818.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHDLD_002135"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHDLD_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFN3363819.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHDLD_002136"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHDLD_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFN3363820.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHDLD_002137"
     CDS             complement(4304..5215)
                     /locus_tag="ACHDLD_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3363821.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHDLD_002138"
     CDS             complement(5232..6239)
                     /locus_tag="ACHDLD_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN3363822.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHDLD_002139"
     CDS             complement(6236..8365)
                     /locus_tag="ACHDLD_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3363823.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHDLD_002140"
     CDS             complement(8370..8480)
                     /locus_tag="ACHDLD_002140"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363824.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZAOG010000116         8416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000116 DAZAOG010000000
VERSION     DAZAOG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.116"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..1076)
                     /locus_tag="ACHDLD_002141"
     CDS             complement(108..1076)
                     /locus_tag="ACHDLD_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN3363825.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1308..2141
                     /locus_tag="ACHDLD_002142"
     CDS             1308..2141
                     /locus_tag="ACHDLD_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN3363826.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2175..3014
                     /locus_tag="ACHDLD_002143"
     CDS             2175..3014
                     /locus_tag="ACHDLD_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3363827.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3040..3600
                     /locus_tag="ACHDLD_002144"
     CDS             3040..3600
                     /locus_tag="ACHDLD_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3363828.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3799..5520
                     /locus_tag="ACHDLD_002145"
     CDS             3799..5520
                     /locus_tag="ACHDLD_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN3363829.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5685..6827
                     /locus_tag="ACHDLD_002146"
     CDS             5685..6827
                     /locus_tag="ACHDLD_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN3363830.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6843..8054
                     /gene="thiI"
                     /locus_tag="ACHDLD_002147"
     CDS             6843..8054
                     /gene="thiI"
                     /locus_tag="ACHDLD_002147"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN3363831.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2886 a         1455 c         1744 g         2331 t
ORIGIN      
        1 tttacgaaaa ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat
       61 ttttgtcaca gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata
      121 aacgaaatca attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt
      181 gctttttgac tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc
      241 ccgccttcat aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact
      301 acagggattt tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc
      361 cccgcaccta aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat
      421 aagtcccctt gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa
      481 atgtccagtc gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact
      541 aagccgatgg gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt
      601 gtcacgtagc gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc
      661 gcttcttcac aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca
      721 agttcattac ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt
      781 gtatcaagaa aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg
      841 gatatacctg atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc
      901 atgatgtgtg gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg
      961 attgtgattt ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc
     1021 atgttttctt tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta
     1081 cagacttcct tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag
     1141 agttttgcta tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta
     1201 ttaaaatgga aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc
     1261 ttttacaaga aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg
     1321 gattaacatc gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg
     1381 aatatgcagg gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa
     1441 agtcctcagt tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag
     1501 tctatcaagg aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga
     1561 tgattgcgca ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct
     1621 ttttagtcca attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca
     1681 aacttagaaa gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt
     1741 acagcgatga atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag
     1801 ccatcatcga tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca
     1861 atccgaactt tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg
     1921 ataaagagtg gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac
     1981 tagcacatga agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata
     2041 ataattctgg tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca
     2101 agccagatgg actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga
     2161 aaggggcgaa gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca
     2221 atcgttcaaa agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca
     2281 ttttatgcgg tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat
     2341 tggaattaga tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga
     2401 ttatcgacca cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga
     2461 accatcgaca aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat
     2521 taggacaatc gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac
     2581 gtctgatgaa aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa
     2641 cgttagagat attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac
     2701 agaaaaagtt agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg
     2761 tagatatcat cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg
     2821 tcgaaattga aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc
     2881 tgaaaggtgt aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg
     2941 cagactttgt gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg
     3001 gtctgattta ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt
     3061 caaaaagtag aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct
     3121 tttacagaaa atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt
     3181 gttgcagcta cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa
     3241 gcattagata aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg
     3301 gttgaacaag tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta
     3361 ctaggaatag tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta
     3421 tttccagatg gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac
     3481 tggcagatgg aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa
     3541 gaagcgattg ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga
     3601 tcagagtttc actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa
     3661 gttagtgaat gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag
     3721 acgtattatt tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg
     3781 aaaacggagg aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt
     3841 atagtcgcca ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga
     3901 ttggatgcct tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc
     3961 gatgatgtta aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt
     4021 caaaaatgga atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa
     4081 gtggaaaacc tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca
     4141 gaagctacaa tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa
     4201 ttgcgggaaa cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa
     4261 gaactgaaag aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg
     4321 caaaaacaga tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct
     4381 ggagaccctg ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg
     4441 gaagctctga tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa
     4501 caacttgaag aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca
     4561 gaagaagatc ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc
     4621 aatcttgaaa aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg
     4681 attgattcgt tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact
     4741 aaccaatcaa cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg
     4801 attgagctgg atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta
     4861 cgaggtttcc aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag
     4921 atccgtcaac acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa
     4981 gtcttagaag atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga
     5041 aaaggcgaaa aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg
     5101 aaacgttttg tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt
     5161 tttgttgcta ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac
     5221 atggaagagg tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag
     5281 aagacacatg atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat
     5341 cgttatcgtc atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc
     5401 aataaagaat atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta
     5461 gaacctggtg ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa
     5521 agcaaaaaag ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt
     5581 tttgtatatt tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga
     5641 caaccgttat aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata
     5701 acagtgcaac aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc
     5761 ggattattgg gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc
     5821 agcaagcaag aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta
     5881 cttcaggcgg aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag
     5941 aatatgggaa tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag
     6001 aacaattaaa agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg
     6061 tacgtgtcga cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg
     6121 ctgtgaataa tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg
     6181 attttcctaa aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct
     6241 cgcaatggtt gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc
     6301 cacggggaac tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag
     6361 gtggagggca agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta
     6421 tggttaaagc tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac
     6481 gcttaaaagt agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag
     6541 aaggaactga ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag
     6601 ttctcgtcca tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct
     6661 cacgtaaaca tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta
     6721 catctgctgt tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta
     6781 tgattatttt caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc
     6841 ttgtgaaata cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc
     6901 gaaaaagctt tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag
     6961 aaatcaaaat ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc
     7021 agctagttat tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc
     7081 gtgtagaaaa agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt
     7141 atactgggaa agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga
     7201 caagtaatga attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc
     7261 aagttcaaat gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt
     7321 atctatcgta tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag
     7381 gaatgcttat gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc
     7441 ggggagtgga aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac
     7501 tgcaaaaagc aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta
     7561 tcgaagtgcc atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt
     7621 ggatgacaat tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc
     7681 atggtttagt cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa
     7741 gtatggtggc tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg
     7801 ataaacttga aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc
     7861 cttttgaaga ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg
     7921 aaaaggtcca tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag
     7981 ctggcttgaa gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt
     8041 cagaattgct ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa
     8101 cgaaaaaata gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg
     8161 aataccattt atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa
     8221 attttattta gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga
     8281 aatagcgagg ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat
     8341 tgcttttcaa atttttgtga aatttcggct tatttttcga agaagttact tctggaacac
     8401 cgtgtatcag aggttg
//
LOCUS       DAZAOG010000117         8339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000117 DAZAOG010000000
VERSION     DAZAOG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.117"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..838
                     /locus_tag="ACHDLD_002148"
     CDS             17..838
                     /locus_tag="ACHDLD_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN3363832.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1123..2133)
                     /locus_tag="ACHDLD_002149"
     CDS             complement(1123..2133)
                     /locus_tag="ACHDLD_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN3363833.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(2145..3098)
                     /locus_tag="ACHDLD_002150"
     CDS             complement(2145..3098)
                     /locus_tag="ACHDLD_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363834.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(3088..4158)
                     /locus_tag="ACHDLD_002151"
     CDS             complement(3088..4158)
                     /locus_tag="ACHDLD_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363835.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(4164..5207)
                     /locus_tag="ACHDLD_002152"
     CDS             complement(4164..5207)
                     /locus_tag="ACHDLD_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363836.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(5208..6149)
                     /gene="opp3b"
                     /locus_tag="ACHDLD_002153"
     CDS             complement(5208..6149)
                     /gene="opp3b"
                     /locus_tag="ACHDLD_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN3363837.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            6233..6580
                     /locus_tag="ACHDLD_002154"
     CDS             6233..6580
                     /locus_tag="ACHDLD_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN3363838.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(6650..8311)
                     /locus_tag="ACHDLD_002155"
     CDS             complement(6650..8311)
                     /locus_tag="ACHDLD_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3363839.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         2368 a         1717 c         1555 g         2699 t
ORIGIN      
        1 caatggaggt ggtcagatga accgttttgc ttctaaagaa gaagaaaaat tatattatga
       61 acataaagca agtgaagaag aatttcttaa ctggtatcaa aaacaggaac gtccagaata
      121 tgacaagccc tctttgactg tcgatatcgt actgatgtgc tataacaaag aagaagatca
      181 gctgaaagtt ttgttgatcc aaagaaaagg gcatccttat cgaaattcat gggcattgcc
      241 tggtggcttt gtccaaaagg atgagtcaac tggagaaagc gtcttaagag aaacaaagga
      301 agaaactggt gtggttattt caaaagaaaa tattgagcaa ctgcatacat tcagtacgcc
      361 taatcgagat ccgcgaggct gggtcgtcac agtcagctac ttagccttta ttggagaaga
      421 accgctaatc gccggagatg atgccaagga agtacgctgg ttcacactag aacgtcatag
      481 taacaagatc catttatcta gcggagaagt ggctatctcg cttgatttag tcactggaga
      541 gtcttctggc aaagatactt tggcttttga ccatagtcaa atcatcttga aagcattcaa
      601 ccgtgtagtc aataaaatgg aacacgagcc gcaagttcta caagttttag gtaaagattt
      661 cacgattacg gaagctcgaa aagtatttgc taaattctta gggatcgatt ataaaacgat
      721 cgatcattcc aattttaaga aagctatgct ccaatatttc gatgagatcg gtgaacgtcc
      781 tgtaggaatc ggccgtcctt caaaaatcta tcagctcaag ccggggcacc atgaataagg
      841 ttgtgagcaa aagattcaga cctaatcatt caagaaaaaa cgcaaaaaca actctttgtg
      901 agttacgttt tttgtttcag aattttgatg tattcgtaaa ctttctgaat aaaaaattaa
      961 caatagcaga aaaagctcgt acgaactttt tctgctattg ttttattggt ttgtcataat
     1021 tagtgagctt ttcttcaaaa gaaaaattcc tagaaacaaa agtcattaag actttttatt
     1081 aacaaggcta accctaacgc tcctatcagt ttcattcatt ttttatccct tgataaccga
     1141 atgtctgccc attttttcta ctacttggct ctttcttacg gaattgcttt cttctgctcc
     1201 gtcggtattg atcaaaagaa attttttatt ataccattgg gacttataac gcaattttgt
     1261 tattaattta tgccagtcgt gaatagaaaa atcagcatgt gcctcttctt ttggaaaaga
     1321 ttctacaaat aagtagtcat attgtttgac caatttccca gataattgtt caagataatc
     1381 ttctcgctgg ttcttttgtt tcagataaag atccattact tttctctttt gtttctgata
     1441 attgcttgcc tgatctaacc tgatctttct atggtgagcg gagcggactt tcacattcaa
     1501 tttccgctgt gcatgttgca gcttttcttc tgtctggtga aattttggta aagttacttc
     1561 tagaggtcga gatgtttgga tcaatgtctt cggatgatag gcgatcccga tccatttagc
     1621 taaagattct ttttttactg gcgaaacatc acaaagtagt gcgacataaa attcttcatt
     1681 atatcgagca gaaatcgtag cggagcgaat cgatccttca actggccgat gctgatttat
     1741 cttgattagt tctttttgtt ttggtaactt taagtagcca ttttccagat agatcgtacg
     1801 ggtttggttg ttggtcgtat aagattgcca catacttttt ttcgttttaa gctttgggaa
     1861 gctggcacgt cccttgaaat aattgcgaaa agcccgatcc aaattaagct gtgcatttgc
     1921 taaagcaaga ctgtctactt cttttaagaa tgggtattct ttttttaatg tagcgggtgt
     1981 gagcgaaaaa ttggctactc ctgtttgttg ccaacttttc tgccgctttt tcagcaaatg
     2041 gttataggta aatcgagcac atccaaaagt acgaatcaat tgctgttttt gttcttctgt
     2101 cggatacata cggaatttaa aagcttttag catctatgtc cctcctagtt agcaaccaga
     2161 agagattcct tttgtcgttt tagttttttt gcgtaatatg ggacttcctg ctcagttgca
     2221 tagacaaaat gttctggtgc gatttcacga agttgacgta cttgtccgtc atctggttca
     2281 ggttgatatt tgattcttct gcgttttctc tcatggtctg gatctggtag aggaatagca
     2341 gataacaaac tttctgtata aggatgaaca ccgtgattat agatttcatc acttgttccc
     2401 atctctaata acaagccgtt atgcattaca ccgattcggt cactaatatg tttaaccatc
     2461 gacaaatcat gtgcaataaa tagataggtc aattgatgtt ctttttggag atcttgcaac
     2521 aaattgacca cttgggcttg gatcgacaca tctagtgcag aaattggttc atcacaaatg
     2581 ataaaacgcg gttttactgc caatgctcgt gcaataccaa tacgctggcg ttgcccacct
     2641 gaaaattcat gaggataacg agtaccgtgg cttggattca atccaacagt tctcaataga
     2701 tcatcgactt tttttgcacg ctcttcagat gatttagcta gtccattaac atcaattcct
     2761 tcggcaatga tatctctgac tttcatacga ggattcaacg aagcgtatgg atcttgaaag
     2821 atcatctgaa catcatgccg gaatttcgtc aaaccttttt tatctttgat cttcgttata
     2881 tcttgaccgt cgaataatat ctcaccatca gtaggttcat tcaagcgaat gatcgtacgg
     2941 ccagtagtgg attttccgct tccagattct ccaactaaac cgaatgtttc tccttcataa
     3001 atgtgaaagc tgatgtcatt tacagcatgt acttcattct tttttcctac attgaaatac
     3061 tgtttcaatc ctttaacttc aagtagttta tttggcatca gcaattcctc cttgaccgta
     3121 agtatgtttt tgtttctctt taaagatagc ccagcgacgt tggatttctt ctggcggtgt
     3181 cactttgggt gcctgcggag caagtagcca agttgctgcc ttgtgtgttt ttgatagttc
     3241 aaaaaatggt ggttcttgtt ctgcatcgat tttcatcgcg aattcatttc ttggatagaa
     3301 tgcgtctcct tttggcgggt tcaataaatc cggtggagaa ccaggaatag catacaatcg
     3361 atcgttcgca ctatctaatg tcggcattga accaagcagt ccccaagtat agggatgttg
     3421 cgggttgtag aaaatttctt ccgatgttcc aacttcaacg attcttcctc catacatgac
     3481 agcgacacga tcagcgacat tcgctactac accaaggtca tgagtgataa agataattga
     3541 tgtatcgatt tttgtctgaa tttctttcag cagctccaaa atctgtgctt gaatagtcac
     3601 atcaagcgct gttgtaggtt catcagcaat caggatttcc ggataacata ccagtgcaat
     3661 cgcgatcacg atccgttgac gctgacctcc tgagaactga tgcggataac tgttgatacg
     3721 tttctcagct tcgggtattc caactaattt aagcaattct aatgcagctt tttgtccctc
     3781 tttttttgaa actttattat gctttctaag ggactcagcg acttgcttcc caatgggcat
     3841 cgtcggatct aaagaggtca ttggatcttg gaaaatcatc gctatttctt taccacggat
     3901 tttttgcatc tctctttctg attttccaac gatatcgtta cctttaaaga ggatttgacc
     3961 agcatctata ttggcgttgc ttgacagcaa gcgcatgatg cttctggtcg tcactgattt
     4021 tccacttcca gattccccaa cgatcgccaa tgtttctcct ttattcaaat gaaaattgac
     4081 accgcgaatc gctcgtacct ttccagcata cgtatcaaaa gagatttcta agtctttcac
     4141 ttctaaaatt ttcgtcatcg tcttcactct ttcattttag gatcaaaggc atcacgcaat
     4201 ccatcagcaa gtaagttaaa gccaatcatg ataaccgata aagttactgc cggaatccat
     4261 aatagataag gcagatacag gaatgtcttg tacccttcgc tcaacatcgt tcctaatgaa
     4321 gctgacggag gcgtaagacc taaaccgata aagcttaaaa aggcttcgaa gaaaatagca
     4381 gatggaatac taaacatcat ttgaataata atgacactag agatattcgg caaaatatgt
     4441 ttgaatgcaa ttttcattct tgattcacct aatgtcaatc cagctaaaac atattcttga
     4501 tcttttaatt tcattgtttg tgcacgaacg atacgtgcca ttggtatcca gtttgtgatg
     4561 gccatagcag ccacgattga aaaaattcct ggttcaaaaa cgaccaacat caaaatcatg
     4621 acaacgaggt ttggaattcc tgataagatt tccaaaaaac gttgcatcac gttatcgaca
     4681 cgtccgccta acaaaccaga aaccaacccg taagctacac caatcgttac atctagtaat
     4741 gcagcaataa atgcgatcag caacgagatt ctcattccca taaataagcg gcttaataca
     4801 tcacgtccca aaccgtctgt tcctaaaaag aaatttacgt ttgaagggac gtttgcttga
     4861 gcatatttat caacaagttc accaccaacc attgcttttc cattcaatcc atcgatattg
     4921 attcccggaa tacgaggagg caaattgata taagccacgt tttgggcagt tggatcatga
     4981 ggagaaacaa agattgtgat cacagagatc aaaatgatga ccaatagtaa aaccattgaa
     5041 ataacggcag ctttgttttt ctttaaacgg cgccatgaat cttggataaa attcaaagaa
     5101 ggtgcagtaa tctgttcacg ttcttcttcg gtattttttt gtaatggaag aaattcatcc
     5161 gcagggatat ctgcaactgt ttcataacgc tttggtatca tttccattta tcctcggctc
     5221 ccttctgaaa cacgaattct tggatcaact agaccgtaca atagatccac gatcaaaata
     5281 acaaaaatca gcataaatga gtaaaggatc gttacagcca tgatcgtagg ataatcattt
     5341 gttgtgattg acttaacgaa ttgttccccg ataccaggaa tagcaaagat attttctacc
     5401 accagtgatc cggtcatcaa tgctacagct aacggtccta ataaagtcat caatggaatc
     5461 aaactatttc gaagaccatg tttaaaggcg atttcccaac ggctcaatcc ttttgctttt
     5521 gctaattcca cataatcact atgcagtact tccaccattt ccgtacggat aaaccgagca
     5581 gagtcagcaa gcggcgacat ggccaacgca attgttggta agattgtgta cacaaagcca
     5641 tcccattttg cgattggcaa aacttgtaat ttgattgcaa agatatattg taataaaaca
     5701 gcaaatacaa agtttggaat ggaacgacct aatatcgcta cgagtgtaga agatgtatct
     5761 gcccatgtat tttgcttcat ggcagagatc gttcctaaaa tcgtccctag gactgttcca
     5821 aagataattg cctgtaaccc taactgcaag gatggtccga ttcttccagc taacaaatgt
     5881 gcgactggct ggtttttaaa ttggaaggaa attccaaaat ctccttgaag caaattagaa
     5941 agataaatcc catattgtac gataactggt ttatccaaac cgacttgttt atttagcatc
     6001 tcgattgttt caggactcaa acgttcctga ttcgtatatg gcgtgcccgg cagcaattgc
     6061 atcaagaaaa aggtgattgt tgcaattaac caaagcgtga tgatcatgaa aaagacacgt
     6121 tttaatacat atttgatata actattcact aatgtttccc cctagctttt gacttctgac
     6181 agatgtttcg ctacattatt agataatgaa ccaaaaataa aggagcaatc aaatgaaaaa
     6241 aaaatctatc ccctacgcag tagcttttct actgattctg gttattttaa taaaaaatct
     6301 catcaaccat tcctttacac tcattcaatt atctaatgat ctattcttat ggtcattgcc
     6361 gtttctgatt atcggcggtt ttttatgggt cttttcttcg ggtttcttcg atcattttca
     6421 gcgatcggtc catcttgcac gaacaaggaa ccgaaagaaa aaaccagaat tttcctcgct
     6481 ctcgtctgcc agctacggta tgtacagctt ttggctgatc attgccggaa tactaattgc
     6541 tctttctgct attttcatgc ttttttcttt gttaggataa gtgaatatga gaacgaggtt
     6601 gcgacatttg ccgcagcctc gttttttctc atgctcacgg tattaacgat tattcttcaa
     6661 cgtatgtcca tttgtaatcc catgaagcgc ctgccgcatg gtgaacgata cctttgattt
     6721 tttcattcac taggtgacct tctgcttttt ggtacaccgg aacgacaccc atatcatcag
     6781 agatgatctt gattgctttt tggaaatcat cccaacgggc ttgttcatcg ccagcgtctg
     6841 ttgatgaagc tgctttaact gcttcgtcat attctgcatt gctccagcga ccacggttgt
     6901 atgaattacc tgtgacaaac aaatcagtaa agctgcttgg gtctgcatag tcagcagccc
     6961 atccgcctag tactgcgtca aaatcacccg aagttgagcg gtcaagacgt acagagaatg
     7021 gaacaggtgt tggtttgact gttacgccat ccaagttttc ttggattgag ttttgaacat
     7081 attcgattac ttttttcgat gaatcatcat cagaagccat caaatcaaat tctaaagaat
     7141 caacgcccaa ttcttttttc gctttttccc aatattcttt tgctttatct ttgtcataag
     7201 aaactaattt tccagcttct tttgcaaagt cttcgttcgt tgttggactt tttaccatat
     7261 ctgatgggat caatcctgta gagacaactg atccgtcacc tagtacttgt tttactaaag
     7321 cttcacgatc gatagaataa gaaatagctt ttcttaggtt cacattgttg aatggtgaat
     7381 tcttttcacg ttgattgaat tcgatatagc ttgtccgcgc ttctttaaca gaagtataag
     7441 ctggatcgtt tgcgttttgc tgtgctaact caccagacaa gatcacgtca tctgcttggc
     7501 catctttgaa taagttcaat gcagtagaag attcttttac tacactgact ttgatttcag
     7561 aaagttttac tgtatctttg tcccagtaat gatcattttt cttataagtc cattctgtat
     7621 ctgttcctgg tccatcaaaa tcttctaatg tgaatggtcc attataaaca gctttatcac
     7681 ttgcagatgc ataggcatcc ccattttctt ccactacgga ttgtttttgt gggaaaaatg
     7741 atgggaaagc aagcagatat tggaaatatg gtgtttgttt cgtcaatttg atttctaatt
     7801 catagtcacc aactgctttg atccctagtt ctgatttatc tttctttcca gctgtaatgt
     7861 cttctgcatt ttcaactggc gcatacaggt aagcatattc agaagctgta gctgggtcta
     7921 ctgttcgttg ccagccataa acgtaatccg ctgctgtaac aggatcacca tttgaccatt
     7981 ttgcgtcttc tcgtaatttg attttgtaag ttaatccgtc ttcactgact tctgccaact
     8041 cgctagctcc tgccggctgt ggtttgctgt cttcatcaag acggtaaatt ccttcataaa
     8101 cgttattcaa tgctgtaaaa ctgattgtat ctgttgcaac tgataagtca gctgtaggca
     8161 tttcttgagg cacgactaag ttaaatacgc ctcctccatc tgctgaaccg ctttttgctg
     8221 aactactgct tgtggctgaa ctgccgccat aacatccagc aagtacaagg ccgcataccg
     8281 caaccaaacc aaatgtaaat ttctttttca ttttacgtac ccctttattg gaattttca
//
LOCUS       DAZAOG010000118         8181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000118 DAZAOG010000000
VERSION     DAZAOG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.118"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1028)
                     /locus_tag="ACHDLD_002156"
     CDS             complement(81..1028)
                     /locus_tag="ACHDLD_002156"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN3363840.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1218..1487
                     /gene="rpsN"
                     /locus_tag="ACHDLD_002157"
     CDS             1218..1487
                     /gene="rpsN"
                     /locus_tag="ACHDLD_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN3363841.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1663..2025)
                     /locus_tag="ACHDLD_002158"
     CDS             complement(1663..2025)
                     /locus_tag="ACHDLD_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3363842.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2036..3157)
                     /gene="alr"
                     /locus_tag="ACHDLD_002159"
     CDS             complement(2036..3157)
                     /gene="alr"
                     /locus_tag="ACHDLD_002159"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN3363843.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3173..3523)
                     /gene="acpS"
                     /locus_tag="ACHDLD_002160"
     CDS             complement(3173..3523)
                     /gene="acpS"
                     /locus_tag="ACHDLD_002160"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN3363844.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3653..5164)
                     /locus_tag="ACHDLD_002161"
     CDS             complement(3653..5164)
                     /locus_tag="ACHDLD_002161"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3363845.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5486..6838)
                     /gene="murF"
                     /locus_tag="ACHDLD_002162"
     CDS             complement(5486..6838)
                     /gene="murF"
                     /locus_tag="ACHDLD_002162"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN3363846.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6991..8067)
                     /locus_tag="ACHDLD_002163"
     CDS             complement(6991..8067)
                     /locus_tag="ACHDLD_002163"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN3363847.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
BASE COUNT         2119 a         1805 c         1520 g         2737 t
ORIGIN      
        1 acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc
       61 gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga
      121 tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt
      181 gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct
      241 gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc
      301 aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg
      361 atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca
      421 gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt
      481 tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg
      541 agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct
      601 gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc
      661 attccggctg cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc
      721 aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca
      781 tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt
      841 acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca
      901 taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg
      961 ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat
     1021 cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt tattttgaca
     1081 aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg
     1141 taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata
     1201 attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag
     1261 aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag
     1321 gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg
     1381 atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat
     1441 tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct
     1501 gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg
     1561 cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg
     1621 tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt
     1681 cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg
     1741 atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa
     1801 caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg
     1861 aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg
     1921 gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac
     1981 gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt
     2041 cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac
     2101 ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg
     2161 tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga tccatacata
     2221 cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc
     2281 gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga
     2341 tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt
     2401 cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg
     2461 gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc
     2521 ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca
     2581 gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg
     2641 tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag
     2701 ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt
     2761 ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt
     2821 gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg
     2881 cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact
     2941 cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct
     3001 cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt
     3061 ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta
     3121 tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt
     3181 ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc
     3241 cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat
     3301 cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag
     3361 cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca
     3421 tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga
     3481 tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca
     3541 ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac
     3601 aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt
     3661 tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt
     3721 ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt
     3781 cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct
     3841 gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg
     3901 ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat
     3961 ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa
     4021 ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca
     4081 tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga
     4141 aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc
     4201 tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc
     4261 cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca
     4321 atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc
     4381 gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg
     4441 ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt
     4501 ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca
     4561 catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg
     4621 ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt
     4681 ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc
     4741 caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga
     4801 tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg
     4861 cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag
     4921 ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt
     4981 tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa
     5041 taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt
     5101 caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt
     5161 tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg
     5221 atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat
     5281 tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc
     5341 aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta
     5401 tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt
     5461 tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa
     5521 ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca agattttgtt
     5581 tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt
     5641 atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg
     5701 cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc
     5761 ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa
     5821 aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag
     5881 ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga
     5941 gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca
     6001 aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta
     6061 caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa
     6121 taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat
     6181 ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt
     6241 tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg
     6301 ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc
     6361 ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac
     6421 caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag
     6481 acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg
     6541 cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta
     6601 caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag
     6661 atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag
     6721 gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg
     6781 tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg
     6841 agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga
     6901 ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg
     6961 caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg
     7021 ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa
     7081 gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt
     7141 taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc
     7201 gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc
     7261 ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata
     7321 atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt
     7381 tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga
     7441 atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt
     7501 aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc
     7561 ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac
     7621 ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat
     7681 ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa
     7741 gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc
     7801 ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc
     7861 acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg
     7921 acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata
     7981 ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg
     8041 tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt
     8101 gatttactct catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa
     8161 cgagccagct tctaaaagaa c
//
LOCUS       DAZAOG010000119         8076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000119 DAZAOG010000000
VERSION     DAZAOG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.119"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..546)
                     /locus_tag="ACHDLD_002164"
     CDS             complement(25..546)
                     /locus_tag="ACHDLD_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN3363848.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            745..4437
                     /gene="nifJ"
                     /locus_tag="ACHDLD_002165"
     CDS             745..4437
                     /gene="nifJ"
                     /locus_tag="ACHDLD_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN3363849.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4626..5513
                     /gene="cdaA"
                     /locus_tag="ACHDLD_002166"
     CDS             4626..5513
                     /gene="cdaA"
                     /locus_tag="ACHDLD_002166"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN3363850.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5510..6679
                     /locus_tag="ACHDLD_002167"
     CDS             5510..6679
                     /locus_tag="ACHDLD_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN3363851.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6703..8058
                     /gene="glmM"
                     /locus_tag="ACHDLD_002168"
     CDS             6703..8058
                     /gene="glmM"
                     /locus_tag="ACHDLD_002168"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN3363852.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2591 a         1493 c         1830 g         2162 t
ORIGIN      
        1 ttttgtccca cgctccttat ttttttattt tgtttggatc aattctttta attcctctat
       61 tgaaatagat tcatcaaaga tttctttacc caagatgaca gttggaacaa atcgaatatg
      121 tgcttgctca gcttcgttga tgacagcttg gctcagctgt tcgtcttttt gttctgtcag
      181 ctttagttta tctactgcaa aatccgctac ttcttggaga gaaagatgtt tccactcgtc
      241 ttgagtatcg aatatttgtt tgatctcttt gatcgctttt tggccatcgc tgattgtgag
      301 ataccggtgc atcacgttcc ctcgctgtaa gctttctttt tctttatcga acaatttgat
      361 cactcgctgc aattgcccgc tttggactgc ttcttctaat agctcataag attcttcaaa
      421 ccattgtcgg caatacggac aagctaagtt gatgaattcg accatttttt taggagcatt
      481 cgatgaacca taaaggattc ctttttgtgt atttgttttc gttgcatcaa tcactgatat
      541 atccatgtta aaacacctct ttcgcttata gtttatacat gtctgacaaa aaaaggcaac
      601 gatttagact gtttgtctaa aaattagatg accaattcaa atagatacat gtaaaatatt
      661 tttataaata aaagaaaaaa atggcatgtt ttacgttata atggaacaga taataagaga
      721 gaatatgaac aaggatgatg aatcatgcga aaaatgaaaa caatggatgg caatacggca
      781 gccgcatata tttcttatgc atttaccgag attgctgcaa tatatccaat aacacccagt
      841 tcgaccatgg ctgaattagt agatcaatgg tcagcagcag gaaaagaaaa cgtattcggt
      901 caaccagtaa aagtagtaga aatgcaatcc gaagcaggag cagctggtgt ggtccatgga
      961 tcattgaaaa ctggtgcact gacaactaca tataccgctt cacaagggct gcttttgatg
     1021 ataccgaata tgtacaagat tgctggggaa ctactgccaa gtgttttcca cgttgctagt
     1081 cgggcagtga cgacaaatgc gttgaatatt ttcggagatc acggagacgt gatggctgca
     1141 agacaaacag gatttgctat gttagctgaa agcagtgtac aagaagtgat ggatttagca
     1201 cctgttgccc atcttgcttc tattgaagga gaagtccctt ttttgaattt ctttgatgga
     1261 ttccgtacga gtcacgagat tcaaaaaatc gaagttttgg aatatgaaga attagctcct
     1321 ctgatcaatc aggagaaatt agcttcattt cgtaaacgag ggatgaatcc caatcatccg
     1381 actgttagtg gtacgaacca gaatccagac atccattttc aacaacggga aacgatcaac
     1441 ccctattatg ataaattacc tgaaatcgtt caaaaatata tgaaagagat caaccgatta
     1501 cgaggaactt cttatgactt ggtgacttat tacggagcag acgatgcgga agaagtcatt
     1561 gtttctatgg gatcagttgc acagacgatt gaacaaacga ttgattactt gaatgcaaac
     1621 ggacggaaaa caggtttttt gaatatccat ctttatcgtc cttttccagt ggaaacattc
     1681 gtgcaaaagt tgcctcatac agtaaaaaca attgctgttc ttgatcgaag taaagaacca
     1741 ggggcaggtg gagaacctct cttattagac gttcaaagtg cgttatatga ttctgaactt
     1801 cgcccggctg ttattggtgg tcgctacgga ttggggtcta aagatgtgac tcccgaccaa
     1861 atctcagcag tttttgatga attgaaaaaa gacccaagca tacgcaaaaa acggtttacc
     1921 atagggatcg tggatgatgt gacttatcaa tctttggaac caaaggaaag tctggatttg
     1981 acagaaccgc aaacattcca ggccaagttt tggggatttg gatctgacgg aacagttgga
     2041 gcaaataaat cagctatcaa gattattgga gatcacaccg ataaatatgc gcaaggctat
     2101 ttttattatg attcgaaaaa atctggcggt ctgactgtct ctcatcttcg ttttggagat
     2161 acgccgatac gttcagcgta tcttgtcgag catgcggacc ttgttgcctg tcacacacca
     2221 gcttacctgc atagctatga cttggtcaaa ggattgaagc caggtggcat ttttttattg
     2281 aatactcttt ggagcgacga acaattagaa acgcatctgc ccttaaaatt aaaacgttac
     2341 ttggctgaaa ataacattag attctatacg atcaatgcca tgagattggc ccaagaagtc
     2401 ggtttgggac gccggatcaa tacggcaatg gaaacggctt tttttaaatt agctgacatc
     2461 attccttttg atgaggtgct tccgctgcta aaagaagaag ccctgaaaag ctatggacat
     2521 aaatcaatgg ctatcgtaga aaaaaacata caagcaatcg atcggacgac cgaactgctt
     2581 catcaagtcg cgatcccaga ccattggaga acgttggaga tgcctgaaaa aatcaataaa
     2641 aggaacactg gttatataca agaaatactt gaaccagtga atgcacagga aggaaatcaa
     2701 ctctctgttg gtacacttgt agcaaatgga atgacaactg gcgaaatgcc tttaggaacg
     2761 gcttctatgg aaaagcgggg cgtcgcttta gaagtacctg agtggattag tgatcgctgt
     2821 acgatgtgta atgaatgtgc ctttgtttgt cctcatgctg ccatccgtcc atttttagca
     2881 gatgaagagg aaatggaaga agcgccagaa ggatttatcg tgagagaaat gcgaggcgca
     2941 gacggcgtaa aataccgtat ccaagtttca gtggaagact gtacaggttg tggtctgtgt
     3001 gtagaggctt gtcccgcaaa agaaaaagct ttagtgatga agccttatga agaacagaaa
     3061 gaacaagctg tgaattgggc ttttgcaatg acattaagac aaaaagcaaa tccagtcaaa
     3121 ggaaagaata ctgtcttgtc tacacaattc aatcaaccgc ttcttgaatt ctcgggcgct
     3181 tgctcaggct gtggcgaaac accttatgta aaattattga cacaaatgtt cggtgatcga
     3241 atgatgatcg cgaatgcgac aggctgttct tctatttggg gcggtgcttc tcctgcttct
     3301 ccttatacga ccaatgaatg tggtcaaggg cctgcatggt caaattcttt attggaagat
     3361 aatgctgaat ttggttatgg tatgcttcta gcggcacaga ctcgtcgcga ggctttggca
     3421 agttcaatca aagaagtatt gccaaaagta tcagaagaac tacgagaatt gatggaagac
     3481 tggttagtac acatgtttga gagtgaagga acgcagcaaa gagcagcaaa actgcaagct
     3541 gcgatcgaaa gcgaacgatc aattttccct gaactggagg atatatacaa gagaaaggat
     3601 ctattcgtaa aaaacagcca atggatgatt ggtggagacg gatgggctta cgacattggt
     3661 tttggcggga tcgatcatgt acttgccagc ggagcagacg tgaatatcct tgttttggat
     3721 aacgaagtct attccaatac tggcggtcaa acctcaaaag caacaccagc ttcggcaatc
     3781 gctaaatttt cggctagtgg aaagtatgct tcaaagaaag atctaggaat gatggcgatg
     3841 acatatggaa atgtttatgt ggcgcaaatt gcttcaggtg ccaatcaaat gcaaacgatc
     3901 aaggcatttg aagaagcaga aaagtatcct ggtccttctc tgattattgc ctatacgcct
     3961 tgtatcacac atggcttgct tggcggaatg aaagaatcgc ttaaagaagc aaaagaagct
     4021 gtttcttctg gttattggtc attatatcga tataacccta caatggtcga aaaaaacaag
     4081 aacccaatga ctttggacta caaaaaacct gatttctctg ctatgcctga tttcatgagg
     4141 aaacaagttc gcttttcctc gctagataac gcaaatccaa atcttgcaaa agctttattt
     4201 gaaaagactg tccacgacgc taaaacacgt ttttacaact atgctgcact gacgggagaa
     4261 ttagaaaaaa tcaagaaacg acttgaacca gaggaatcta tcgatgtacc cgttaagact
     4321 agaaaagaaa aaacgacgga ccttgaagca gaagaaagaa gagctgctag aagagctgcc
     4381 agagcagaac gacgtaaaaa aatggaacaa gctacaaaag aaaaaaagga aaaataacag
     4441 aaaaaatata atgaataatt ctctgaagtt taaaaatatt tattgcagag aaaaataaaa
     4501 cgcatacttg caattttctt gaaaattatg cgtttttctc aagaaaaata cgagcatatc
     4561 agcctctaaa attaaaacat atgatatact agagaggaaa tctcaaaaga gaagagaggc
     4621 tttttatgtc atttcaaatc ggggaacttt ttaatttgga ctattggcag caaatgtttt
     4681 ccggtgactt gttctctctg aattttttgg tgaacatact tgatattctg gttgtctggt
     4741 atcttgtata caaactgatc caactgctgc gaggcacaaa agcgattcag cttctgaaag
     4801 gtgtagctat ttttattgtt atccgaatcg tagcggaaat cattggtctg cacaccttgt
     4861 cttggctgat gaaccaagtc attacgtacg gggtgatagc tgctatcgtg atttttcagc
     4921 cggaaatccg gagaggactg gaacatcttg gacgcagttc gcttttcaaa caatcaaaaa
     4981 gcgaacagca ggaagatgag aagatgatct tgtcttttga caaagccatc caatatatgt
     5041 ccaagcgaaa aatcggtgcg ttgatcacta tcgaacgtca tactggatta gatgagtata
     5101 ttgagacagg gattgcttta gatgcagata tcactgggga gctgctgatc aatatcttta
     5161 ttcctaatac accgctgcat gacggtgcag ttattgtaaa agaaggaaag atcgcagtag
     5221 caagcgccta tcttcctcta tcagaaagta tgttgatccc taaagaattc gggactcgac
     5281 accgggcagc tgtcggtata agcgaagtca gtgacgcgat taccatcgtt gtctctgaag
     5341 aaaccgggga cgtcagcatc acgttagata atgaactgat ggctggctta tcccagcaag
     5401 aatatttggc aatcttgcgt agagaactaa ttgctgaaga gaaaacaaaa aacaagaaaa
     5461 acgtgattca atctttccta gacggtatga ctaagggagg ggatcgcaaa tgatctcaaa
     5521 agagaaacgg acaagcattc tttacagttt tattgcattg ctgttcagta ttgcgctgta
     5581 cttcaatgca aatggccagt ccgttccaaa tacgctttct ggaaatgaag cgtataatca
     5641 aacagttacg ggggtgccga tcaagatttc ttatgattca aagcgctact atatccatgg
     5701 atatgaaaat gcagtgactg tcaaactttc cagtgcaaac cgagttcagt tgaatgcaga
     5761 ggcaaacgaa gatacacgga tgttccgcgt cacagcagat atcactaagc tgggcgaagg
     5821 aacacatgaa gttccgctaa aaattcaaaa tttgagcagt gctgtcacag caaagatcga
     5881 accatcgact attacagtaa cggttgagaa aaaggtaata aaagatttcg atgtggaaac
     5941 gttgattcct tcaacgttca caccatctgg atatgaactt gatgatactt ccgttagtcc
     6001 taaaaaagtc tctatcacta caggtgacaa aacattggca gaaatcaaac gagtagtagc
     6061 tgatgtggac gcttcgatgg tcacagatga tgggattaat agtgaggtcc caatacaggc
     6121 gctgaatgca gcaggggaag tattaagtat cgtctctgat ccagtgcaag taacagtgag
     6181 agctgattcg atcaagccaa gcaagtcggt acgactttat gggatacagc aaggaacacc
     6241 agctgccggg gtagaaagct atgatttcag tttttcaaaa ctggaagcag aagtaagcgg
     6301 ctctagcgat ttgcttgcat cgatcggtga cagtattgct gtaccgatca atgtctcagg
     6361 tatcaatcat cgaacaactc gtaaaattga aataccagta gaagaggggt taagtatcaa
     6421 gcccgaatca gttagtgtgg agattacacc cgtgttgcaa acgtcaagca gtagctctgc
     6481 ttcgacgagc agtcggacac aagaaaatac aagtacgagt agaagtattt catctgctga
     6541 ttcaggaacg gaaccatcga caacaaccat ttcttccagt tcggaatctg ggacaagcag
     6601 ctcgaccaca agtacagaag aaacatcaga aacaagcgaa tcaacacaaa tgagcagaac
     6661 aaattcctca gaaaattaag ttaaaggagt aattttacaa ctatgggtaa atattttgga
     6721 acagacggcg tacgcggtga agccaacaag gaacttacgc cagaacttgc atttaagctg
     6781 ggacggtgcg gaggttatgt cctaagccag catgaagaag aaggaaaacg cccacgcgtg
     6841 ttagtagggc gggatacaag aatctcaggt caaatgttag aagaagcgct gatttcagga
     6901 cttttatcgg tgggtatcga agtgttccag ctaggtgtga tctctacacc aggtgttgct
     6961 tatctgacac gtttgcaaaa agcaagcgca ggggtcatga tttctgcttc tcataatcca
     7021 gcacaagata atggaatcaa atttttcggt gcagatggtt ttaaactagt agatgaccaa
     7081 gagctagaaa ttgaagcatt attagatgct gaagtggatg aattgcctcg tccatcagct
     7141 gaaggattgg gaactgtaga agaatttcca gaaggcttat tgaaatattc tcaattcctt
     7201 gtacaaacga tcaacggtga cctttccggt ttgaccattt gtgtcgatgc agcaaacgga
     7261 gcaacttcaa caagtgttaa tcgtttgttt gccgatcttg aaacagattt ttatacaatg
     7321 ggcacaaatc cagacgggtt aaatatcaat gacggcgtag gctctaccca tccagaacgt
     7381 ttagctgaga tggtcgttga aaaaggagca gatgctggac tagctttcga tggcgatggc
     7441 gatcgtatta ttgcggtgga tgaacttgga aacattgtcg acggtgataa aatcatgttt
     7501 atctgtgcta aatacttggc agaaaaaaat cgattgaaaa aagatacgat cgttacgact
     7561 gtcatgagta acttaggatt ccacaaagcg gtggaaggaa tcggtttgaa agatgtcgtt
     7621 acacaagttg gtgatcgtta cgttgtagaa gaaatgcgca aaaacgatta taactttggc
     7681 ggcgagcagt ctgggcacat gattttctta gattttaaca caactggaga tgggatgctt
     7741 tcaggaattc agttactgaa tatcatgaaa cagacaggta agaaattatc agagcttgca
     7801 gcagaggtaa caatctatcc gcaaaaatta gtgaatatcc gcgtaagtga taaaaacggt
     7861 gcgatggatg ttccggcaat caaaaaagtg attgaagagg cagaagctga aatgggatca
     7921 gaaggaagaa tcctagttcg tccttccggt acagaaccat tacttcgagt gatggcagaa
     7981 gcgcctactg atgagaaagt taattactat gtcgataaaa tcgcggcagt agtcaaagaa
     8041 gaaatcggac tggattaaga gatattttca taaaaa
//
LOCUS       DAZAOG010000120         8002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000120 DAZAOG010000000
VERSION     DAZAOG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.120"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACHDLD_002169"
     CDS             384..572
                     /locus_tag="ACHDLD_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN3363853.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACHDLD_002170"
     CDS             650..1663
                     /locus_tag="ACHDLD_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3363854.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACHDLD_002171"
     CDS             complement(1730..2764)
                     /locus_tag="ACHDLD_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3363855.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="ACHDLD_002172"
     CDS             2879..5104
                     /locus_tag="ACHDLD_002172"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3363856.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="ACHDLD_002173"
     CDS             5122..7005
                     /locus_tag="ACHDLD_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN3363857.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="ACHDLD_002174"
     CDS             6986..7960
                     /locus_tag="ACHDLD_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN3363858.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2582 a         1460 c         1697 g         2263 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga ca
//
LOCUS       DAZAOG010000121         7881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000121 DAZAOG010000000
VERSION     DAZAOG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.121"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1011
                     /locus_tag="ACHDLD_002175"
     CDS             148..1011
                     /locus_tag="ACHDLD_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3363859.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1140..2762)
                     /locus_tag="ACHDLD_002176"
     CDS             complement(1140..2762)
                     /locus_tag="ACHDLD_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3363860.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3011..3532
                     /locus_tag="ACHDLD_002177"
     CDS             3011..3532
                     /locus_tag="ACHDLD_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN3363861.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3718..5448)
                     /gene="spxB"
                     /locus_tag="ACHDLD_002178"
     CDS             complement(3718..5448)
                     /gene="spxB"
                     /locus_tag="ACHDLD_002178"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN3363862.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5613..6431
                     /locus_tag="ACHDLD_002179"
     CDS             5613..6431
                     /locus_tag="ACHDLD_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN3363863.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6534..6731)
                     /locus_tag="ACHDLD_002180"
     CDS             complement(6534..6731)
                     /locus_tag="ACHDLD_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363864.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6900..7478
                     /locus_tag="ACHDLD_002181"
     CDS             6900..7478
                     /locus_tag="ACHDLD_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN3363865.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7573..7710
                     /locus_tag="ACHDLD_002182"
     CDS             7573..7710
                     /locus_tag="ACHDLD_002182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363866.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2361 a         1403 c         1562 g         2555 t
ORIGIN      
        1 tcttatttct tgaagctgac cacttctgtc acaacctctt attttgatta gaacttgcga
       61 aaataaaccg gttaaagtgt gtctagcgca agagatttgc tataatagac gataagggta
      121 aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg taacagattc ttgttgtgat
      181 ctgccatacc atgtattagc aaaagaaaaa gttgattttt tatcaatgaa tctccaattg
      241 aatgatcaag agttagtaga tgacctcgga aagacattca attatgaccg ctacatgaat
      301 cagctgaaaa atggtgcgat gcctacgaca tcacaaatca atgttggccg atatttagaa
      361 tttttccgtt cttatgcgga aaaacagatt cctgttttat atgtggcttt ttccagtgga
      421 ttaagcggct cttacagtag tgcgcttcaa gcggttgaat tgctgaagga agaatatgaa
      481 gaccctgaga tctatgtttt tgatacaaaa gctgcaagta ttggggaagg gcttttagta
      541 ttggaagctg taagattgaa aaaagaaggt aaaagcatcg aagaaaccct agagtggctg
      601 gaaagtaatt atttgaaagt ccattcatgg gtaacagtag acgatctgaa atatctagaa
      661 cgaggcggta ggatttccaa agcaagtgct actcttggca gtctattgag tgtaaagcct
      721 attattacgg tggataaaga aggaaggctg acgaatgttg acaaagttcg aggaagaaac
      781 aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa tcgttgcacc acttgatcaa
      841 atcgtttatg tggcttacgc tggggatcta gagagcgcgg agaaggcaaa acggcttttg
      901 gaagagcaga taaagatgaa agatgtgaag ctttatccct taggaccaac gatcgccagt
      961 cacacaggct atggctgtat cgcgattttt tcgatgggaa tcagtcgata aaagcagtaa
     1021 ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa acagcgactc ttaaaagata
     1081 gatgagcatc ctatctctta gaggtcgctg tttttttatt ttttaagcta aataggccat
     1141 tatgcccata actcgttcaa ttgtttttga atatctgcat tttccaaaaa ttcatcatat
     1201 gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg caatgatgcg gtttgccaat
     1261 gtttggataa attgatggtc atgtgacgca aataagattg agccagtaaa tgccatcaag
     1321 ccgtcattca atgctgtgat tgattctaag tctaaatggt tcgttggatc atctaataca
     1381 agtacgttag ctttcgaaag catcattttt gaaagcatgc aacggacttt ctctccacca
     1441 gataaaacat tcactggttt caatacttct tcaccagaaa acaacattct tcctaagaag
     1501 cttcttaaga aggtgttatc atcttcttct ttacttgcat attgacgcaa ccagtctaaa
     1561 atcgttagat cagtatcgaa ttctttcgtt gtatcctttg gtaaataaga attacttgtt
     1621 gtcacacccc aacgtactgt tccagtatct ggcgtaattt cacccatgat aactttgaat
     1681 agcgttgttg ttgttatatc attttctgcg acgaaagcga ccttgtcttc tttgctcaaa
     1741 ttaaaagata tatcatctaa aattttcttt ccatcaatcg ttacacttac attttctacc
     1801 tgcaataagt cgtttccaat ctcacgttca ggtttgaatc caacgaatgg ataacgtcta
     1861 gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca tttttttacg tgaagttgct
     1921 tgttttgatt ttgatgcatt cgcactaaag cgggcaataa attcttggag ttctttgatt
     1981 tgttcttctt ttttggcatt tgcgtttgct tgaagtttag ccgctagttg actagattcc
     2041 aaccaaaaat cataatttcc tacataaagt ttgattttac caaaatctag atccgccata
     2101 tgtgtacata ctttgttcaa aaagtgacgg tcatgggata caacgatcac agtattttca
     2161 aaattgatta aaaattcttc cagccaattg atcgattgga tatccagacc gtttgttggt
     2221 tcgtctagca aaagaacatc aggtttacca aatagtgttt gtgctaataa cacttttacc
     2281 ttttgtccac cagttaattc actcatttgc tgatggtgca attcttcagg gatgttcaga
     2341 ccttgcagta aaacagctgc ttctggttca gcttcccatc cattaagctc agcaaattct
     2401 ccttctaatt cagctgctcg aataccatct tcgtcagtga aatcctcttt catgtaaatc
     2461 gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc ccatgatgac tgtttcaagt
     2521 acagtatagt cttcatagtc gtaatggttc tgtttcaata cggccatccg ttcatctggt
     2581 cccatagtaa cggaacctgt ggaaggctgc agatcccctg ataacacttt aaggaacgtt
     2641 gatttacctg caccatttgc accaatcaag ccatagcagt ttccgggggt aaatttaata
     2701 ttcacatcat caaatagttt gcggtctgaa aagtgcaaac tcacatcatt tacagtaatc
     2761 aatttttttc ctcactttta tcattattag ttttgacaag tcttatagaa ttatataggg
     2821 gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt ttcacgtgaa acagaatgaa
     2881 aaaaataaaa ataaaagcgg agcgaaaata gctactatag gatttattag tttgcaacag
     2941 cataattttt gacgttattt ataagaggga gtactataaa actgtaaaag aaatgaaaga
     3001 aggattgaaa atggatgaaa aaagaaccat cgactggggc tcattgattt tgggaatttt
     3061 atttgtactt gtctcattgt tatcatttca agaccctgtc ggaaatttag ttgcaattgt
     3121 agtagtcttt gcgatttttg cctttattaa agggatattt gaactgtttg tacggaacag
     3181 attaaaagag ctaacaggat ataaaggaaa gatgccctta ataatcggga tcatcgatat
     3241 tctagttggc gtctttttct tattcaatat tggtgcagga gtcgcagcat tgccttttgt
     3301 ttttgcagta tggtttattg ctgattcagt tttagcgtta tttactgctg acttggcaag
     3361 aggagtaagt gaaggatatt attggtttac gatcatcgtc aatattttag gaattttatt
     3421 aggaattttc ttattgttta atccaatttc ttctgcgttg acattaagct ttttagttgg
     3481 attctatttt atgttatttg gtatcacaca tatcgtctat gcatttagat aagtaaaatc
     3541 aaacaaataa aaaagccggc ttccgattac agttttactg ttgtaattgg aagccggctt
     3601 ttttatcatg gacaaatatg cacgaggtga tttttaggtg tcgagacaaa tttatgcaaa
     3661 aatgtgagaa gctatataca caaattttat cttaatactc gaagcatagc gcagttgtca
     3721 caacctcttt tcatataatt cattcaatac tggcaaatca tggacttcat atttttcttt
     3781 gaatgcttgg atttcttctt cagaatattt tcttggatct aaatgcagat cttctactgg
     3841 caatgggcgc ttgttattga tcttcacatc gattactaca ggacgatctg attttgcagc
     3901 tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact gtaaagctat ttgccccgag
     3961 cgcttctcca actttaccga aatctgcatc ttctaaaaat acaccaaatt tcttttgttc
     4021 cgtatcttct tgttctgctt cgatgaaacc aaaagaatca ttcgaaaaga caacattaat
     4081 aattggaagc tgatatttga cttgtgtaat gatatcttgc atgaccatag caaacccgcc
     4141 atcaccactt aaggtaaata cttgtttttc tgggtagctt agttgtgcgg caattcctcc
     4201 tggcacacca ttacccatag ttgcgaacca tccagatgtc gtatgttttt gtttaccgtt
     4261 catattcaat aaacgaatag aatggatagt cgtgttaccg acatctgtga cgaaaatcgc
     4321 atcgtcttca gcaatacgat tgatttcttt atagacagcc tctggacgaa tcggttcttc
     4381 ctctcgatct tcaaaagatt tcaaccaatt tacccatttt tctttatttt cttgatttgc
     4441 taggtaccag ctatcagcag ggcgagcatc ccctaactcg acaagtcttt gcaaagctgt
     4501 gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt cgtccaaatt ttgacgcatc
     4561 gatatcgatt tggataaatt cagcagttgg attaaaaaat gcacgtccaa atgggaagtc
     4621 gctaccgaca aataagatca gatcggcttc tgccaatgct tcatttccgg gtttagtagc
     4681 gacacgtcca gcaaaaccaa gaaagttttc ataatcatca ggaataattc cttttgctag
     4741 aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat tctttgattt gcggaaatcc
     4801 gtttcgcgtt ccttgaccaa cataaagaac tggttttttt gctttttcaa gataaggcaa
     4861 agcagctagc aaatcttttt cttctggtaa tatcacactt gttttgtgag tgtgagcggt
     4921 ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca acagggatag tcacgatagc
     4981 cacgccttta tgctcatagg cggctttaat ggcttcatct actacatgcg gaagactttg
     5041 tggtgtcatg actgtacgat tgtatacgct aacatctgcg aaaataggat tttcatttag
     5101 ttcctggaaa gagttatagt tcatggatgt agaagcaact tgtcctaaga acgctactag
     5161 aggcacatgg tccatctgtg catcatacaa accattgatc aaatgtgtcg caccaggtcc
     5221 tgcagagccg aagacagcgc cgactttccc cgttaatttt gcatctgcag cggctgcgag
     5281 tgcgccgact tcttcatgac gaacttgaat gtagttgatt tcggtttttt cttgatatag
     5341 cgcatccata attgaattga aagatcctcc gggaatgccg taaatatggt cgatacccca
     5401 gctttcaaga acttttacca ttgcgattcc tgcattgatt tttgacatgg tgtttcactc
     5461 ctttagcaat gatgatacat aaattttttg tatctagagt atagaacttt ttatcgaaaa
     5521 tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt tttgggaata tgctagagta
     5581 gtacatagat agattcaaga gaggggaatg gcatggttga atggctattg agacttgaac
     5641 cttggcagca agcacttgct gggacagctt ttacttattt tatgacagct cttggtgccg
     5701 ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct aaacttgatg ttaggatttg
     5761 cttctggtgt catgattgct gcaagttttt ggtctttgtt agatccggct attacaaaag
     5821 cagaagaaaa tggagatatt gcttggctag ttgtaagtat tgggtttggt ttaggcggtg
     5881 tgtttttata tatggcagat aaaacactgc cccatatgca ttttggacca caacatgaag
     5941 cagaaggatt gcctacccat ttaaaacgga cgatcttgct tgttttttct attacattgc
     6001 ataacattcc tgaaggttta gccgtggggg tagcattcgg tgcagcagca actgcggata
     6061 atccgacagc cgctatatta gccgctgttt cggtggcgct aggtattggt atccaaaact
     6121 tcccagaagg agcagctgta tcaatcccgt tgcgacaaga aggtctgagt cgtaaaaagg
     6181 cctttgttta cggacaagct tctggaatcg ttgaaccaat tgctggagtg attggagcgc
     6241 tactagtaac gaaagtggag ctgctgcttc cttatgcact tgcttttgcc gcaggagcaa
     6301 tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca gcagacattg tccagcaaac
     6361 gtcattttgc agtttttggc gtcatgagtg gatttatcat tatgatgatt ttggatgtgg
     6421 cattaggata aataaaaaaa atatatatga tatatcaaat agggccgcga acttatatcg
     6481 cgaccctatt tttttagtaa gttatatcat aatgaagtga atggtatcat acattaataa
     6541 tagttttttc tatgtgtatt tcctttgatc cagcgaacaa gccagatgat aagtgcgatt
     6601 gggattagaa ctttgaccgc aaaccataaa agcccgctta ggacactgac aaaaagactg
     6661 atgacgaagc tagctgcaat tagcactaaa agaaccatga agatatttaa gaacatattt
     6721 ttttgacgca tacaaaaccc tcctcttgtt cattctttac tatagaaaaa gtataacagg
     6781 gattgtttaa aaacacctcc aactgaagac tgatttttac ttcatctact taaatgatca
     6841 aacaattgct ttttctctgg tttatcatct aaaatagagc ggaaaaggag gagaatatca
     6901 tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat cgcgattatc atgactattt
     6961 tagttttaga acttcatgaa cctaacggag acacgtggga ggcgctcgct gcattaggtt
     7021 accgattttt tatctatatt gtcagtttta tcattttagc gatttattgg aacaatcatc
     7081 atcatttatt tcaagttgta cataagataa atggacgggt cttatgggcg aacagtttct
     7141 ttgtttttgc gctttcgctt tttccgtttg caacttcatg gatcagtgag tacatcaata
     7201 gcctggctcc agaaatgact tatggttttg ttaccttgac cgctaatatt gcgtattata
     7261 tcttggcaaa agaacttgtc aaagcaaata aaggccacga gaaagcattg aaggcgatgt
     7321 tgaataatta caagaaatcg tatctatcga ttggtttgaa tgtgttagga attgtcttag
     7381 ggtatttagt agcgccagta gccgtgctga tcgcaaacat cgtgattcta gctgcttgga
     7441 tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta
     7501 agagaaaaat atagaaaaaa taaataaacg atcaaatatc cgagtgatac tagtattcaa
     7561 agagggatgc agatggatca aaaaagaatt catgtaggac tgattactag ttttgtcacg
     7621 atgcttattt atttggatca aggtcatttg acttggacag tagttggtgt cgtaggattt
     7681 ttgacattat tcacagcaat aaaaaaataa acatctttat gtaatctgat gttctatcta
     7741 tttaagaaaa gcatgtttac agcagtatga ttctgttgta aacgtgtttt tatttgtgga
     7801 aaatttaatt tttagaaaag tgtatcgaaa aatagatagt tagtttttcc acagtggata
     7861 acttcgtaag agataaaaat a
//
LOCUS       DAZAOG010000122         7810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000122 DAZAOG010000000
VERSION     DAZAOG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.122"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1584
                     /locus_tag="ACHDLD_002183"
     CDS             46..1584
                     /locus_tag="ACHDLD_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN3363867.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1581..2546
                     /locus_tag="ACHDLD_002184"
     CDS             1581..2546
                     /locus_tag="ACHDLD_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363868.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2533..3855
                     /locus_tag="ACHDLD_002185"
     CDS             2533..3855
                     /locus_tag="ACHDLD_002185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3363869.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      3918..4022
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4096..4839
                     /locus_tag="ACHDLD_002186"
     CDS             4096..4839
                     /locus_tag="ACHDLD_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363870.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            4854..5846
                     /locus_tag="ACHDLD_002187"
     CDS             4854..5846
                     /locus_tag="ACHDLD_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3363871.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            5858..6595
                     /locus_tag="ACHDLD_002188"
     CDS             5858..6595
                     /locus_tag="ACHDLD_002188"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363872.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6585..7307
                     /locus_tag="ACHDLD_002189"
     CDS             6585..7307
                     /locus_tag="ACHDLD_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN3363873.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7391..>7810)
                     /locus_tag="ACHDLD_002190"
     CDS             complement(7391..>7810)
                     /locus_tag="ACHDLD_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN3363874.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2502 a         1368 c         1707 g         2233 t
ORIGIN      
        1 ggaaagaaat gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata
       61 caaacattaa gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt
      121 tctctcattt ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc
      181 aatcataaaa aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct
      241 tcacttgcct tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg
      301 attgcacttg ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct
      361 gaaattgacc cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc
      421 ttgctgaaag tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc
      481 ttagttttaa tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg
      541 acatttattt tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc
      601 agttctgcct tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa
      661 cctcgatatt ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta
      721 tatcagtctg ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa
      781 cctgaaatcc tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt
      841 gaattaaaac cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa
      901 atcgatatct atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa
      961 gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg
     1021 aataagcaat tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg
     1081 gcagtcaaga gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct
     1141 atctctgatc agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat
     1201 aaaggaatcc aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt
     1261 ttgcgttacc aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat
     1321 agccagctca aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct
     1381 tatcaaggcg caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa
     1441 gcactgaata aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa
     1501 gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga
     1561 ttagtaaagg aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt
     1621 atgatggaaa gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg
     1681 ttttggttgg tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat
     1741 ctaaaccatc agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc
     1801 agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga
     1861 cagtcaagca aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag
     1921 cagaccgtgt agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc
     1981 gtatgcctag agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg
     2041 ctgcaagccc agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa
     2101 gaaactcatt acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt
     2161 tcgttacgca caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg
     2221 atggagaaat tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact
     2281 acgttcggca tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag
     2341 tcgtcaatgg atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg
     2401 atacgcctat gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag
     2461 aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag
     2521 aggaggaagg aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta
     2581 agtgctgctt atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta
     2641 tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta
     2701 gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc
     2761 aactgggaag aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga
     2821 aaaaaacaat tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat
     2881 gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc
     2941 gggcaaagac ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac
     3001 tttttatcat tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct
     3061 tttgagctag ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat
     3121 caacggccat taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa
     3181 gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact
     3241 cgctatcgtt tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact
     3301 gggagacagc cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa
     3361 cacggaattg cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga
     3421 gatattgttt caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat
     3481 gtagctaagc ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg
     3541 atcgtctacg ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg
     3601 tcagatcaag tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa
     3661 ccagttgcta aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg
     3721 attagcgagc aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg
     3781 actaaaaaag agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc
     3841 tatttattga aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct
     3901 acactttaaa aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg
     3961 tgaacttaga cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg
     4021 cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag
     4081 aaaggaggta acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta
     4141 attatttggc agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct
     4201 gctgctgtat tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg
     4261 acattaaatg aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc
     4321 ttggcaatgg attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca
     4381 caaatgttgc caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg
     4441 cttccaaaag tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg
     4501 gatacattga ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat
     4561 acagggaaga tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt
     4621 ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct
     4681 caagcaggct tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt
     4741 tttgctgcga tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta
     4801 ttgaaaaaag ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa
     4861 aattatggct tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa
     4921 caagtagcaa acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac
     4981 cgaatactaa tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg
     5041 gtttaaatgt ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg
     5101 cggacaaagc acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg
     5161 gacagaatat accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg
     5221 ccaccctatc agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag
     5281 gttggcaaag tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg
     5341 acttttcaaa actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat
     5401 cctcagatat ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta
     5461 ttgaactaaa ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg
     5521 taattattac gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg
     5581 atgccacgaa aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt
     5641 tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt
     5701 caaaaaatta tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca
     5761 attatacagc ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac
     5821 tattcactaa tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt
     5881 gaatgtagtc attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga
     5941 tacctttaca tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt
     6001 aacaggagtc cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg
     6061 tctccataca gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt
     6121 ctatcaaaac gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt
     6181 aagggattat ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt
     6241 atctggcggg atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta
     6301 cttgcttctt gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga
     6361 ctggatgttg aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat
     6421 cgaagaagca atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt
     6481 gattgctgag attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc
     6541 agagttgaag caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc
     6601 attgtcattt agatgggagt cagagtttag cactccttca gcaggaacac acgattttga
     6661 cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta
     6721 agactcaagc cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac
     6781 aagttgagcc ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt
     6841 ggacgaatgt accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag
     6901 ccataaatga gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt
     6961 atatccaagc ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac
     7021 aagactatat tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag
     7081 cagtctggca actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac
     7141 tacatgcagc agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc
     7201 ttgaagaaca gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa
     7261 aaaatactta tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga
     7321 cacaactagt gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa
     7381 taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac
     7441 cataacgtga agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac
     7501 catttaagta agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt
     7561 aagaaccgct tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag
     7621 tagcagcagt ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt
     7681 tttccgtttg aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa
     7741 cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt
     7801 gtcctgagta
//
LOCUS       DAZAOG010000123         7782 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000123 DAZAOG010000000
VERSION     DAZAOG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.123"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..337
                     /locus_tag="ACHDLD_002191"
     CDS             <1..337
                     /locus_tag="ACHDLD_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363875.1"
                     /translation="SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKS
                     ISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNN
                     NFYFYRNKL"
     gene            complement(463..1140)
                     /gene="nth"
                     /locus_tag="ACHDLD_002192"
     CDS             complement(463..1140)
                     /gene="nth"
                     /locus_tag="ACHDLD_002192"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFN3363876.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1147..1845)
                     /locus_tag="ACHDLD_002193"
     CDS             complement(1147..1845)
                     /locus_tag="ACHDLD_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN3363877.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2113..3297
                     /locus_tag="ACHDLD_002194"
     CDS             2113..3297
                     /locus_tag="ACHDLD_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN3363878.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3303..3746)
                     /locus_tag="ACHDLD_002195"
     CDS             complement(3303..3746)
                     /locus_tag="ACHDLD_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN3363879.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3972..4259)
                     /locus_tag="ACHDLD_002196"
     CDS             complement(3972..4259)
                     /locus_tag="ACHDLD_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3363880.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4430..5782)
                     /locus_tag="ACHDLD_002197"
     CDS             complement(4430..5782)
                     /locus_tag="ACHDLD_002197"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN3363881.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6055..6501)
                     /locus_tag="ACHDLD_002198"
     CDS             complement(6055..6501)
                     /locus_tag="ACHDLD_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3363882.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6737..7663)
                     /gene="cysK"
                     /locus_tag="ACHDLD_002199"
     CDS             complement(6737..7663)
                     /gene="cysK"
                     /locus_tag="ACHDLD_002199"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN3363883.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2105 a         1583 c         1270 g         2824 t
ORIGIN      
        1 ttctgtactt ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat
       61 gattctcact tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa
      121 accgcccaaa tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat
      181 catttttatt acacctttag atagatacgt cacttattta tataatcctt tggaatactg
      241 gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa
      301 aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga
      361 gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa
      421 caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt
      481 ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa
      541 aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag
      601 tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac
      661 ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg
      721 ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt
      781 tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa
      841 acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc
      901 aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt
      961 cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc
     1021 acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc
     1081 ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat
     1141 attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc
     1201 tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac
     1261 ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt
     1321 aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg
     1381 ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg
     1441 tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc
     1501 ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag
     1561 attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt
     1621 tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc
     1681 aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac
     1741 aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga
     1801 tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct
     1861 caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact
     1921 tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga
     1981 tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt
     2041 tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa
     2101 aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct
     2161 gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg
     2221 ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat
     2281 gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt
     2341 catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta
     2401 ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa
     2461 atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt
     2521 tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt
     2581 ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt tactttctta
     2641 aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta
     2701 gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt caagatttca
     2761 tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa
     2821 tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta
     2881 agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg
     2941 ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc
     3001 tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt
     3061 atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat
     3121 tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta
     3181 tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg
     3241 acggtattcg gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag
     3301 cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca
     3361 aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg
     3421 agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct
     3481 tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt
     3541 agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga
     3601 tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat
     3661 tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc cagatattct
     3721 tctattagca gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt
     3781 ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa
     3841 gaaaaaaagg ggttgacaaa gcttgtcata accccttttt ctgattaaaa gatagtaaag
     3901 agacagtcat cggtaattgt gatgattttt tgtcatcact taaggatgag cggccttttc
     3961 aaaaactgtc tttaaagatc atcatcagtt tctagcacat atggctgacc ttcatatgtg
     4021 tactctgtat tcaatgtgcc gtcattttca tctgagtaga tgcgtctact gaaaggaaaa
     4081 cgatgagaaa cattatcttt ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt
     4141 tcattcaact gagagagtac ttgatctgcc aactcatcaa tattgatcgt taatttcttc
     4201 gaagcacttt tataatcttc taaaaatgca tcttgttgga caaaatattc tttattcatc
     4261 ttctttctca cctttctttt caaaatcaaa taaagctgct tgttctttta tgtaacagct
     4321 ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag acaactaatt gtacttttca
     4381 caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac cctttttctt tattgtgcag
     4441 tttcgatttc gcctagcgct gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa
     4501 aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt ttattttgaa
     4561 cagctaaaga catggtattt gctgattgtg taatgtcgta tttggacatg aactgagcac
     4621 ctacgatacg attcgttcct ttttcataga ctagctccat cattactttt tcagtagttg
     4681 gcatgaactc tggacgataa ttatcttcaa atacagtcgc ttggacttct agatcgttca
     4741 aagttgcgct ttctttggtc acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc
     4801 ctgaagttcc ttgagtacca cgatacgcca ttttatgatc tgtcaagtta tttcccacaa
     4861 gcaaaccttg acgaacggca tttgttgcca aaggaatata gttttgcgtg ccacttggat
     4921 tgtagttgac tactgcagag tctccagctg caaaaatgtc tggattactt gtctgcatgt
     4981 attcattcac tttgattgcc ccgtttggca gtgtatctac ttttccttcg atcagttttg
     5041 tatttggacg gaagcccaca cataaaatga ccatatctgt atcaaatgta tctgttggtg
     5101 tagctacttg tttcactttt ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta
     5161 gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg aatggtttat
     5221 ctagatattt attcaagatg cgatctaatc catcaatcaa tgtcacttgt tttccagatt
     5281 cgacaaaagc ttctactaat tcaataccga tatatccacc accaacaatt actacacgtt
     5341 ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt gtagtttttg cataggagta
     5401 catttttcgc atcgattcca ctaatcggtg ggataatagg ccatgaacca gttgtcatca
     5461 ctaatttatc ataagaaacc gtttctgttt ctcctgtttt cagatctttt gccgttactg
     5521 ttttattttc tgtatcgacg tctgttacat cgtgttccat tttaactgtt gctcctaatg
     5581 aagataattc ttcaggattt gagtagaaca agcctgctgg gtcctttact actccgccga
     5641 cataaagtgc gataccgcaa gataagaaag aaatattgtc atttctttca tatactgtca
     5701 cttctgcttc tggatgattt tttaaaatac tttttacagc tgcagtacct gcatgagtac
     5761 agccaattac tactactttc atttatggtc cctccgatta acattttgtc cttcgacaaa
     5821 actactataa cagaaataaa acggatacaa cagtttaatg cttgtgaatt cacaaaggtt
     5881 tgtttgtagt ttcattcctt ttcaagcata agaacccaat atctacaaga ttcttatttt
     5941 aataatattc acttttatat ctaagattgt gaataaaaga caaaaaaata aaaaagctag
     6001 cgtttttttt acacagctag ctttttttag gtataacact tgtagaaact gtttttacat
     6061 ttgcgccttt tttgctgact ctttacaatc cggacagagt ccatacaatt ccaaccgatg
     6121 ttcattaatc tcaaaatcag tcagtctgct tgcagccatt tctatatctt caagccctgg
     6181 gtaatgaaaa tcaacaatct taccgcagtt ttcacaaatt acatgataat gacgctttga
     6241 actgaaatca aatcggctag aagcatcgcc gtacttcatt tcttgaacaa atccaatcga
     6301 agtaaataag cgaaggttat tgtaaacagt cgcaacactc atatttggaa accgatgttc
     6361 aagtgcttta taaatctcat cagccgttgg atgggaatga tgttcaatca aatattctaa
     6421 aaccgcgtac cgttgaggtg tgattcgaat gttctctttt tttaactttt cgataacctc
     6481 ttcgactaac ttattttcca tgtaaacacc ccacgttgtt attctcgtct tatcatacta
     6541 tttttacatt ttaaaatcca cttaacatga aaagaaaatt caattttatt gtattttaat
     6601 gaaaaaagac gtaaaacagt cgcgttcact attctacgtc tctattcaaa taatttttct
     6661 catcataaat ttttgtgtgt tcttttcatc tttaaaagtt ttttacataa acctatctgg
     6721 agaaaaacca agtgccttat atctcttggt aaagggctgt agataaataa cgttcaccat
     6781 tgtcgggaac cacagctaat accttcttgc cttttcctaa ttcttttgcg acttttagcg
     6841 cggcagcaat tgcagcgcca gatgagatgc ctactaaaat cccttctttt cgcccaactt
     6901 ctctagctgt ttccatcgct tcgtctccac taatagaaag gactttatca tacacagaag
     6961 tatccagtgt tttaggaaca aaacctgtac cgattccttg gattttgtga ggtcccgctt
     7021 ctttcccttc taaaatagca gattctgctg gttctacccc gattaattcg attttcggat
     7081 acacacgttt taattctcgg ccagcgccag tgatcgttcc tcctgtaccg atacctgcaa
     7141 caaacgcgtc caagccattt actccaaatg cttgatgtat ctcaggacca gtcgtcttct
     7201 catgcacaag aggatttgct tcattttcaa attgtaatgg taaaaagtag ccgttttctt
     7261 ttgccaaacg ttctgcttct ctgattgatc cagaaatacc atccgcacca ggagtcaaaa
     7321 tcaactctgc gccgtaccct ttcatcaata aacgtctttc gatactcatc gtctcaggca
     7381 tcacgatgat tactttatat ccttttgcaa ctcctaccat cgacaatccg attcctgtat
     7441 ttccagaagt aggttcaatg atagtatcac ctggtttcag tagaccatcg tgttctgctt
     7501 tttctatcat acttaaagca atacgatctt ttacacttcc tcctggatta aaaaactcaa
     7561 gctttacaaa aacatctgct gaatcttctg gtacgatttt atttaatttg acgattggcg
     7621 ttttgccaat caattcggtg actgagttat agatagctgt catcttcaat tcctccttgt
     7681 ctttgctctt cttacttatc ataatacaat ctgttcattt atggcgagtg attcgtcttt
     7741 gactagcaaa aaaaagacaa agatatacca tatatggtaa tt
//
LOCUS       DAZAOG010000124         7741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000124 DAZAOG010000000
VERSION     DAZAOG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.124"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1235
                     /locus_tag="ACHDLD_002200"
     CDS             207..1235
                     /locus_tag="ACHDLD_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363884.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1304..2644)
                     /locus_tag="ACHDLD_002201"
     CDS             complement(1304..2644)
                     /locus_tag="ACHDLD_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3363885.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(2803..3243)
                     /locus_tag="ACHDLD_002202"
     CDS             complement(2803..3243)
                     /locus_tag="ACHDLD_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3363886.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3403..3909
                     /locus_tag="ACHDLD_002203"
     CDS             3403..3909
                     /locus_tag="ACHDLD_002203"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN3363887.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4077..5990
                     /locus_tag="ACHDLD_002204"
     CDS             4077..5990
                     /locus_tag="ACHDLD_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3363888.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5995..6996
                     /locus_tag="ACHDLD_002205"
     CDS             5995..6996
                     /locus_tag="ACHDLD_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3363889.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7198..7404)
                     /locus_tag="ACHDLD_002206"
     CDS             complement(7198..7404)
                     /locus_tag="ACHDLD_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN3363890.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2719 a         1245 c         1562 g         2215 t
ORIGIN      
        1 ggttatattt tactggattc actttacaaa ggtacttatc aatccttaga aaatcttagt
       61 caaaaaatta tataagtttg cgtacgattc aaagaaaatt aagagaactt aatactattt
      121 tgaaaaacta tcatttatct tgcagcttga aaaaaggtaa tccgatagaa ggagaagaat
      181 accgtatccg atactttttt cacttgatgt attggcagtc attcgacgaa aatcattctg
      241 atatattatc aagtataaaa aataaacgtc aactattaga aaaatttgcg aaacatgcgc
      301 catatacgcg tagtatagat agaaagaaat ggatcagcct actaagaatt tcgttgcaac
      361 gaatgaaaca aggccactcg attacgtatg tcccagaaga gatgaacaaa tttgtgcatc
      421 cattgataac aaaagaagag tttacgcatt acatactaga gccttttttt gaagcaaact
      481 tctggtcgat gaatcacatc actccaacag agcaagctta cctttatttt atgtttggtg
      541 taatgaacac ctatttggaa gaagatttgt tggatactcc agataaagta caagaaagtt
      601 ggggctggat tgaatcgtct attgtagaaa attttgaaaa aaacttcaag atttgtttta
      661 caacttcaga aaaaaattat ctatatgtca atttggcaat gatccatttg tattcaaggg
      721 tttttgggac aaaaaagaag gtggatgctt ttggaaaatc agcagaagac agagattttc
      781 agcatatatt tccagtgatg tatccaatca tgcaacagtt ttttagtggg gtagctcgca
      841 gaattccaga gcttaatact tattatgaaa agaacagacg gttgattttt caatactgta
      901 tgttagtcag ggatattttt ataaaacacg aacaacctgt catattctat attcaatcaa
      961 agtatggtaa aacacaggaa ttatgggcaa agaaaaggat attatctatg actgaacgcc
     1021 caataaaata tagtgcatcc gctgatcagt tacctgatat tgtcattagc gattatccta
     1081 tcgacaaaaa ggcatatgat caaacgaata cacagatatt ttactggaat ggacaaccta
     1141 caaaaaatga ttgggcgcgt ctgaaagctg aaatcataaa aatcaggaat gagaaaaata
     1201 gatccatatt ggaaaaattg gatttcgtta attaagaaaa gcggctcttc gtcaatgagt
     1261 gttggtgaag aaattcaata gaataatcga gatactcaca cggctagaca gctatcttgt
     1321 ctagccgtgt tgttgttctg gctttagtca gttgggtagt gttcttagtt ttcttgaaat
     1381 attttcctcg atataaaaag attcgttctc ggtagttttg aaagtttcta aagccatagg
     1441 caattcgttt tagtactttg atatggttgt tcaaacattc tatagggccg ttggaatagg
     1501 gaactcgcaa cgctcgtttg atttctgttc gatatttctt gaaggtcgtg aacactggtt
     1561 gatagtgctc tggcaactcc ttgtagtctt cttttaggag cgcgacgaat tcaggaacat
     1621 cttgtcttct gatagcggaa agaaaaacct ggtagacttc gtagcctcgc tttaaggagt
     1681 catcataata taatagccga tcgactatct ccgcttctgt cagatgatct cgaaatgacg
     1741 gacgccagta gcgatgctga ccattcaatt cccacgcatt cttttgaagt agtctccagt
     1801 atttctttaa gtgcttcgac cgacgatccc cttttttaaa ggtcttcatt acttgaattc
     1861 ggtggttggt aaaggcccga ccgatgtgtt ggctaatatg aaagcgatcg gcgacgattc
     1921 gggcattcgg aaaacactct ttcaccaatg tgcgataggg tgtatagaaa tctgaaacca
     1981 ctagacgtac ttgttcacgg acttttctag ggtagcgcaa gaaatagtcg cgtaaaggat
     2041 tgagttgccg attttccaca atatcaatca gttgatgggt cttgccgtcc atcatgacaa
     2101 agctcatggc tcccgagacg tttttcaccg atttgaattc atcgaaacac accacttctg
     2161 gtaacggctg gttcaaagaa acggttttag gccgaaggct gcgaagtatt cgaatgacag
     2221 tggtcggaga aatgtgcttc atacgtgcaa tcaaagacat agaaataggc tcacttaata
     2281 gctcaagaat ggcttgtttg actctgcggg caatggagca gcgacgatcg ctaactgaat
     2341 cttctgctaa aaaagtcttt ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca
     2401 caatggtctt gtagttactg acatcattca ataagatgga tacttttttc cagccccatt
     2461 taatgatcgt ttgcttattt tgatagtggc aatggaagca ttcctctggt tgataggtca
     2521 atgtgccatc aaaaacaaaa catgtttcgt cattaatgcg ctctttgcga aaggcatctt
     2581 catgaaaggt aagatttagg tctagaatat ctagtaactc tttggtaaac tctgaatagg
     2641 acatcttgaa acactccttt gaatggtttt cgtcgacttt attctaaagg atttcaaggt
     2701 gtctgtttgc gtttattaga aaaaagaaaa agtattggca acagaaggcg tcaccaacac
     2761 tagatattat agatccagaa aagcagattg tttatgccaa aattaagaca ttgcaaaaag
     2821 attattttct ttaaaatcga caagatgacc agaaagcaag agttcgttga gctcttgctt
     2881 tcttttagtt ttagaaagga acatcctgat tttatcttcg ctaaatccag tcaaaagttt
     2941 tgcagattgg tcgtagataa tcggtttttt caataaagaa ggggatgtac gaatctcaat
     3001 atatagttca ctcaaactga gttcatctat tctgtgaaca agatttttat aactaataga
     3061 atgagtagag agcatttcgg tcacgccgtt gtttgtcaat tttagtaagt ggataaattc
     3121 atttttggtt agtggttgac gaattaagtt ctgttcgata aacggtatat catggacgat
     3181 aagccaacgt tttgcgcgtc ttgaagacga gcagccagaa cctgtataca atttaatcat
     3241 catacattcc tccaatagct aagtatttat gaaataataa catgaagaaa tagtcgaaaa
     3301 gtgccattta gaggtgtaaa ataagaaaat gttgtcacgt ataatggaac tagctagata
     3361 agaaatacta ttaaatcaag aaaaaagaaa gaaggcacaa gtatgtcaaa cacagccgac
     3421 gcaatagtag tgatcgattt acaaaaaggg gtatgttttg attcttcgcc gatacacgaa
     3481 ttagaaacag tgacagcatt agtgaataaa agaatcaaaa catacgaaac gattgggaaa
     3541 ccagttattt tcgttcaaca cgaagatgct caattagtgc cacaaacaaa accatgggag
     3601 ctattgtcgg aattatctat tcctgagcac agttattttg tgagaaaaac ccatgcgaat
     3661 tcattttata agactaattt gagtgtttta ttaactgaat tggactgtca ttcgatcgaa
     3721 ttatgtgggg cacaagcaga gtattgtata gatacgactg tgaagtttgc tcatggatta
     3781 ggataccagc ttatgatgtg tagaggagca acgacaactt atgataatcc ttttatgaaa
     3841 gcaaaggaaa caatcgcttt ttacgagaaa atttgggata aacgatttct gacatttttg
     3901 gagcaataag ttgtttcttc gtttaaaaaa agcagaatgg atactggttt atccattctg
     3961 ctttattacg taaaatgaga aaataatcct gttctatttt tatgggataa accatttatt
     4021 ttttgtataa aaaatgtttg cgtttacata taattctcct agaaacataa aaaggagtga
     4081 atagcttgag aacaaaacaa gtattaaagg gagcgttggt catcttattt gctgtaagta
     4141 caagcgtccc gcaatggaca gtatatggag agagcatttc ttccgaatcg gaatctcggg
     4201 aggtagaaac cacagaaact actgatgctg gaaatgaaac gaaatcgagt acttcacaaa
     4261 cagaaacaaa tactgaaaca acggttgaga catctgttac tagcacagat caagaaacga
     4321 ccgctaccac agataaccaa caaacagaag aagcaacagc tacaaaaatg tcaaacgacg
     4381 aatcctcttc tgctaaagta agtgacaaag agaatgggac agaaaatgat gatatcggta
     4441 aaggtgaaaa tttcaatggt aaagaatatt tgaattactt cggtaaacag atgacagaaa
     4501 tacttaatgg accaacagaa gacatcaagt caaaattaat agagttatta gtcactgagc
     4561 ttgctgttaa tgattatggt gtagataaag agattatcaa acatctgatt gctacttatc
     4621 tattgtacga tatcgctttt gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag
     4681 atgatgggac aacgcctaca gaaaacacag gcttctatcc aaatacaaaa caacttcatc
     4741 aaactgtact aaacaaacaa gatggcaaaa tgatggatgt atatgcatat tatgtcgatc
     4801 aaggttcaga taaaacagtc atgatccatg gcggatttcg agggaattgg aataacggga
     4861 tcgttacaga agaatacaat gatttttata aggccgggta taatttatta tttgttgatt
     4921 ccagagcgac tggtaatagc ggaggcgatt atgtgaccta tggacaatat gagtcagatg
     4981 acgtccttta ctggataaat caagaagttc gagagcggcc atctcaaaag attttacttt
     5041 atggtggttc gatgggagca gctacgatga tgtctgtatt agcgaaagat attcctgtca
     5101 atgtcaaagg aattattgaa aattgcggat ttgcatcaat tgatgaacaa ctacgtttta
     5161 cgtacagcca gacagtagcc cccgcactac cggatgccat aaaaaatcag ttagacatca
     5221 ttggggatca agagcatgaa gatttattta tgggcctttt gaaacaatac tattttgatc
     5281 aggagatgca tttggataca aaagctgctc tacctacgat tggaatgtca gattcattgc
     5341 ctaaattgat tatccatgga acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat
     5401 tatatgagtt atcaggaggg tataaagatc ttttattagt agaaggagca gggcatggaa
     5461 aggcacagga agttgaccat gctgcctaca cgaaacatgt aacggacttt ttgaaagtag
     5521 tgttccatga tcagataaac gtaaagtatg tggatgaaaa caataaaagt ctactcaaag
     5581 accaggacga aatccagctt tacggagctt atggagaaaa ttatatgaca gagcaaaaga
     5641 cattcgaagg atacgaatta gcaaatgttg aaggaccgac tgaagggatt tttaatgaga
     5701 cgataccaac tattattttc tattacaaaa aaatcccagt agctccacca aaaaagcaag
     5761 atccgacaga gaatgcagac aataagggaa agaatgaacc tcgagaggat caatcaagtg
     5821 atgatacgca accgattcag atgacagtga aagtacaaaa aaagacagat gcaaaaaaaa
     5881 gaatgttgcc gaaaactgga gaaaagaaac attcgcttct catttattca ggtggatttt
     5941 tatctgttag cacgattatc acatggattt ggcgaagaaa gagagggtaa atgaatgaaa
     6001 tggttcaagc gaatagtgat tgcagtacca gttatcatcg tagttatttt agtcactgta
     6061 tttttgatta atcaaatcac accaaagccg attagcttgc ttgttagaaa acaatttgac
     6121 acaagtgaaa aagaacaagt atatgcccga ccagatgatt tccagcaaaa aatcgaacaa
     6181 atcgatataa aaaaagaaat atcctatcct tcaaagtaca aaaataataa tatggatatc
     6241 tacacaccga aagtggaaaa gaaaaaatta ccgattcttt tctggatgca tggaggcgca
     6301 tatgtaggag gggataaaaa tgattgtcgt gattatttgg aatatctatg ttcagacacg
     6361 cagcaaatca tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca tcctacacca
     6421 gttatccaat tgaacgaagc cattcaagca gctaaaaaag agataaaaga tcaagcagat
     6481 tggtcgaata tttcgattgg tggaaattcg gcaggtgcac aaattagtgg agaatatttg
     6541 ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac tttggagaac
     6601 aagcagatta cgaagtttat ttctttgtct ggtctgttgg atccttctgc acttactcaa
     6661 gtaagtgata aaatctctag ctttctttat gaaaaatgtg gatgggctta ctttgataat
     6721 aaggactttg aacaatcaga gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt
     6781 tggacgcaaa acacattctt aacggatgga aatacaaata cgtttacgaa acaaatggaa
     6841 gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag
     6901 aaaaatgctg ttttgaacca tgaatatcag tttgatttca gcaataaaca agcgcaagaa
     6961 acatatcaaa agttagtaat gtttttcaag gagtaaagtc ttatgatgca aaagaggttg
     7021 taatctgtca cttagctcca agtcacaagg agcaatttca ataaacaagc ttttcatggt
     7081 ttatgaatat tgtaacttgt gactgaggag ttagagatgg caaggggcac cttttattcg
     7141 aaatttggga atatcacacg attattgtca cgatctcttt tacgtaaatt ttataagtca
     7201 gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag
     7261 ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc actaatattc cgttgttatt
     7321 gatataaggc tgggaacaaa gatgaatagc aaataaaatc aatgcactag cgaaaaaaag
     7381 caaagaaata tggatagctt tcataagcag cacctccttt tttataattg tatgataaat
     7441 agattaagaa tctataaatg tgacaagttt gttaggatgt gaataaaatg atcatctagc
     7501 gaacttgaaa caaagatggc taatgataag caattaatga ttcagcaatc agataggtac
     7561 aaagtttacc atcatacgtc tagaccaata atagaaaata tgggaatata atagaaaaga
     7621 tttaggatac ccccaaaaga atttccccaa aaattcgatg tatcctaaac ttttttattt
     7681 tacataacaa tcatatcaac tgtgaaataa aattacaaat gataacctaa aatgaggcca
     7741 a
//
LOCUS       DAZAOG010000125         7681 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000125 DAZAOG010000000
VERSION     DAZAOG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.125"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..2388
                     /locus_tag="ACHDLD_002207"
     CDS             61..2388
                     /locus_tag="ACHDLD_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFN3363891.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2385..3299
                     /locus_tag="ACHDLD_002208"
                     /pseudo
     CDS             2385..3299
                     /locus_tag="ACHDLD_002208"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3372..3887
                     /locus_tag="ACHDLD_002209"
     CDS             3372..3887
                     /locus_tag="ACHDLD_002209"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFN3363892.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3884..4330
                     /locus_tag="ACHDLD_002210"
     CDS             3884..4330
                     /locus_tag="ACHDLD_002210"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN3363893.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4480..5295
                     /locus_tag="ACHDLD_002211"
     CDS             4480..5295
                     /locus_tag="ACHDLD_002211"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3363894.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5501..6673
                     /locus_tag="ACHDLD_002212"
     CDS             5501..6673
                     /locus_tag="ACHDLD_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3363895.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6835..7542)
                     /locus_tag="ACHDLD_002213"
     CDS             complement(6835..7542)
                     /locus_tag="ACHDLD_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363896.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
BASE COUNT         2590 a         1450 c         1530 g         2111 t
ORIGIN      
        1 aaaattaaat ttagaataac gtgtaaagga ttatttgatg attttttatt gagaggaggg
       61 atgaaaatga ttaagaaaac tcaaaataaa atccaacgta tatgtcaaaa acattttgtt
      121 tctagactca aagctgtatc taaaagaaaa tacttacatc ccattatgat ttcgatcatc
      181 ttgattttta ctttcttagg gagcttatca agtatttatg taaacgttta tacagaaacg
      241 tcaaattttg ctaaccaaac gacagaggag acaaatagtc caccctattc tgaaatacag
      301 tccatagaaa gcaccaatca gtctagcaac aacgagacaa cctcaaccac atcctcgact
      361 caactgattg atccgacaga aaaagaaatc acagcagcaa aagcagcagg ggagaaagct
      421 tattcagaaa caggtagaag ccagacattt agtactgttg cggccactgc gaatttcaac
      481 ggacatacca taccagatgg atatacagtg atatatgcca aagatggagt cattgttgcg
      541 gttaacggat cgatgagtgc ggataaaact gcgttacaag gcgtaaccaa agcaatcgct
      601 tttattgata accgtccaga tatccataat ctgtttattg gctccacagt aaatggtact
      661 actatcacat tcccaaaaag tatctatatg ggggtggcta ccgctggaaa tacgatcgat
      721 tcagttacac ccaatgcgga taaaagttat tcttttacac cctcaacatg gggagggctt
      781 cctgccggag gaggtatgag tactttttca atcggtatac agttagcaaa tgatattcca
      841 ttcacggggt actcgatcaa aaatgggcag atcgttgatg gtaagggaaa tgtttcaaat
      901 tttccaacct atcaagcagc aacggttcag gtcaatgcga atcggacagg aactttttct
      961 tatacagtca atggtggagc agcaaaaatc ggaacgtacg ggactacatt atctggaatc
     1021 catgtgggtg acaaagtggt tatcactcca acagcagtaa cgggctattc ttttgcacaa
     1081 acaaacggtt ctttcacagc cctagaaagt aacgtcgata cggtgaccta tacaggcaaa
     1141 caaacaccaa tcactattaa gtatcaagac tattttggac agatgattgc tccttctaaa
     1201 ataatgaatc ttacttacgg aagtgctgca caagacctga cgactaatac gcctactatc
     1261 aacggatata cctttacagc tgtttcagcg atagaaacaa agaaccaatc agcaatgagt
     1321 gttttagcta gtttagatac aaatggaaat gtaatcgtaa aggacgcaaa tggaaaacag
     1381 atttctgaag tcatcctata ttataagaca aatgcaacag tatcaatcaa tgcaaatggc
     1441 agtaagtact atgatggtct ttctgttgta ccgatcgtta aatatgcctt taatgataaa
     1501 acagaatcaa catccctttt aaacaatgat ctcagtattg atcaaatcac atggaatcca
     1561 actgatttca aagcgatgaa tgaaaaggga gttgaagtaa ctgcgcctac tgaaattgga
     1621 acctatacgt cttggcaatt gacaaaaagc ggactagcta aactagctgc cagaacaaac
     1681 tatttattta ctatcgtgca gacatctgat gtctatacga ttaagcaaat cagcggtgcg
     1741 gtagagctca gtggcagtaa gacatatgat ggaaaagcag gtgtcccgtc aatccatgtc
     1801 aagctagcag agggtgtcac tagttcgctt actccagtac aagtggcact tagcagtatg
     1861 gattacacag cagatgcaca atcagcaaaa aatatggtta atgctggcag ctatacaatt
     1921 aaactaacga atagcggaat agacaaagtc aaagcagcaa atcccaagtt gtcatttact
     1981 gatatagcca acacatcagg aacttatacc atctataaag ctaacgcagt gattactgtc
     2041 gatgacgctg cctttaatta tgatgcccaa agtcactcga tttcggttgg aaatctccac
     2101 gttaccggtg ttgtcttagg cgaatcattg gattataccc taacagttaa cagccggaca
     2161 gatattggat cacaaatagt aacgattagt ttaggcgaag gaagtgtaaa caataattat
     2221 acgattatcg caacagatac cgctaagttg acgatcaatc cgactctctt gcttccttcg
     2281 acaggtggga taggtctact gccctttgtc tttttaggac tgatctttat tttttctggc
     2341 ttcttttatt ttatccatcg taagaaaaaa gcaggtgaac agcgatgaaa aaaacaaaaa
     2401 atgtacgtgt ggatatattt gctgttctct tttttatcct actggtcttt tttataggtt
     2461 ctccgactgt gtcagctaga gaatcaccta ttacatttac tttgcataat cgaatgttcg
     2521 aaagcgataa gatgccaagt atcagtcaaa acgacggaat aaatatcccg gataactttc
     2581 aaggagtaaa agggttgaac ggcgttactt tcgctatata tgatgtctcg gatgaatttt
     2641 acaagctgcg gtcagaagga agtagtgtgg aagacgcaca aagaaaacta gcacaaaaaa
     2701 gtgatagtga aaaaatactg gcagaaaatg tgacaaaaac agtagacgag gaagagggca
     2761 ttgcttcttt tagtgcatcg gacaaagatg agcaaggtcg tgacgctgtt taccgattta
     2821 cggaaataaa gacttcagat taagtaaaag aaaaatcagc accatttgtc gttgtgcttc
     2881 ctgtgacaga ttcatctaat cataaattga cgaccatcca cttgtatcca aagagcgaac
     2941 aaaaaatacc tgctgcactc acattaacaa aaacagtaga gaataagcag acagattttg
     3001 cagatgggga taaagtccct tatctcatca ccgtaacaat tcctgagaac atcaacgaga
     3061 taggaaccta tacgatcaaa gatacagctg atcctcagtt atggctagaa ccagaaacga
     3121 tcgatttttt gattggtgga gacaagattc atacatttca cacacaaaaa ataaaacatg
     3181 gttttgtctt atcagattct ggaaaagatc ttagttcgtt tgctggaaaa aaattaacga
     3241 tccgttacca aatgacattg aaagaaaaca gtgaaaaaac gacatttgat aacgatgtac
     3301 gtttgacgac tgacaatcaa acagtagaaa cacatcttgc catccaaaca ggaggcaaac
     3361 aatttgtcaa agtggatcgg caaacgaaac aaaaactagc agatgctcaa tttctagtca
     3421 aaaaaaatga ccaatactta gcgcaagaaa acgggataaa tcattgggta gccaaagatg
     3481 attcaagagc aacgattttt acttccagta aagatggaac attttctgtc catggattgt
     3541 cttttggaag ttacgaacta gtggagatta aaccgcctaa aggatatata ttgagtcaat
     3601 caaccattct ctttgaagtt gaaaaaggca gttatgatcc gcttcatcct ataccattgg
     3661 aaatcatcaa cgaaccaaaa gcaaatccga cgaataatgg aaaaacgcca gttcagatgc
     3721 agacaaatgg tccatcaaat gaaaaaaatg gaggaagctt tccaaaaaca agcgaagaga
     3781 tgcttggagg tttctcaatt ttagggttat tgctagtcct ttcaacaggg acagcttggt
     3841 tttataaaaa acaacaaaaa ggaaacaata ggagggaaat aaaatgaaaa acatcaaaac
     3901 aaaaatatgg acgtttttag gaactgctat catgctgctt cctttcgttc tcggcctagg
     3961 aactgcagag gtttctgcag ccgtatcacc tacaccagaa aatgttacgg ttaatttgca
     4021 taaattgaaa ttcacttcag caccagaaaa tcaaataaac aacggaacgg aattgacatt
     4081 tccgaatagc gaaccgttga acggcgttga attcaacgta tatgacatca ctgctacgta
     4141 ctatccaagt aaagacacag cagttccagc agacgctaca cccttcgcaa gtgtaacgac
     4201 ttctggtgaa ggattagcta acctgactct tccaggtaaa tctgatggga aagatgctgt
     4261 atacgtcttt gtagaaacac caaagcccgg tgtcgaaacg tcgccaaata tcgtgttaag
     4321 cttaccctag accctgacaa acctttagac acgattcacc tatatgctaa aaatgtcatc
     4381 aaagaaagca atatcaaaat ccagaaagct gggaactttt caggggtaga taagttagca
     4441 ggggcgcaat tccagatcaa aaattcagag aatcaattcg tgaccggttt tgatgaaaac
     4501 actggtgtcg ctctctatga tcctgctgag tcagcgttga cattcacaac tgatactgaa
     4561 ggatccatca gtgtcaatgg attaatagac ggcaattata ctttagttga aacaaaagca
     4621 ccagatggtt atcaattaac caatccacaa aaagaaacta cttttatagt aaaaaacgga
     4681 aaattgcaac aaacaaccgg tatcatccat ggcgcagatg aagccaatct tgggaatcca
     4741 aacggtgtag tcaatcggga aaatacaact acggataaca cgattactgt agaaaatgtc
     4801 caaaactacg gtgatttcca cttcatcaaa caagatgtga acacgcaaga aaaattagcc
     4861 gatgcagaat tttctattct aagagcaaaa gatccaactg ttaaattaat gaaaaaaata
     4921 gcaaaccttg cctcaggaga atacccatat gcttggtcag atgaagtcac aggttctgac
     4981 tgggaagaag tgaaattgat ttctgattca gaagggaaat tttcaatcga agggctgaaa
     5041 caaggggcat actgccttca agaaacaaaa gcaccagaag gctacgcatt acctaagaat
     5101 gaagctgcgt acactgaatt tacagtagat gatgatccaa caacttctga tgaaacaacg
     5161 atcgataacc aacctaaagg aatcttgcct tcaactggtg gtatggggat catcgtattc
     5221 atcttggtag gtactgcttt agttggagga gcagtgatct atttcaaaaa acgccatcaa
     5281 gaaacagaag cttaaactat tggaaacaaa aatcatacga taaataaacg atagtgattc
     5341 aaatggggag aaaggttgtg acaagtggtc ggtctcttgg tattcgctca acaaacggca
     5401 aaataggagg aactattttg ccgtttgctt cgctaatact caggtcaagg cgctgatttc
     5461 acaacctctt catatacata ttagaaaata ggtggacatg atgaataaaa gcagaataag
     5521 aatactggac atcttcatgg caatcatttt agtcgttggg atcgggatct tttcttatcc
     5581 ttttgtagaa gattcactga atgattttct tgcccagcaa atgatcatcc attatcaaaa
     5641 gcaagcatcg aataagaatt cagaggaaat aaaaaaacag caggaaaaaa tgacaaagaa
     5701 aaaccaacag ttggcagagc aaaacgtatc accagggata ggcagtttca accaagcagt
     5761 tgatgcaaaa gcattaaaag atttacctag taatgccttt tttatggaac atatgcttgg
     5821 agtcatcgaa atcccaaaaa tcaatgtcag cttacctata ttcgatcaga caacggaaat
     5881 atttttgcaa aaaggaacgt cattattgga aggaagttct tatccgactg gcgggaaaaa
     5941 cacccatgcc gttctttctg ggcatcgtgg actcccagaa gcaaaattat ttacagattt
     6001 gcctaagttg aaaaaaggag accagttttt catccagatc aatggaaaga cattggcgta
     6061 tcaagtagaa aaaatccaag tggtcttacc tgacgaagta gattctttgg gtatccaaaa
     6121 aggcagagat ctcgtgactt tgcttacctg cactccttat atggtcaata cacatcgact
     6181 gcttgtgacg gggcatcgta ttccttatca agcaaaagag gcaaaaaaag cgatccaagg
     6241 aattgatcaa tggaaaaaat ggaaattttt tatttggttt atcggtatac tacttggatc
     6301 aatcggctta gtttggctac tcatcgctta tttagattct ttggccatcg ctaagcgaaa
     6361 ctatccttta tctttttatg taaagaacac aaacggacga ccaatcgaag gaatggtctt
     6421 ttctgtaaaa acgctcaacg gcaaacacta tattactaga gaaaaagttc catttgtgaa
     6481 agcttctgat gaatacggac tagtgagatt ttctgattta aaaggcagga actatcgctt
     6541 gcaacatgag gagctcctgc ttaaaatcca tgtaaaacat aaacattcga aacagttttc
     6601 aatgaaattg aaaaaaggaa gatataaact gcgaaaagaa aaagaggtgt actatttgat
     6661 tgagaaagaa tgaatgaaga tgtccaaaaa agcagaggga acagactaaa tgaaaaaaga
     6721 gagaagctga cgaaatggac gtgtaaactg ctatttttgg tttactatct ggaacgccag
     6781 cttctatttt tttatgtttc agaagaattt tattattata gtataacttt tgaattagtt
     6841 tccataacat ttggagcaag gggttaaacc acgagcttga gcttcgctta aagtggcagg
     6901 ggtatacgta ccatttccac atttatgtgt atggtatttt gaaccggtcg gtgtgacata
     6961 gacagtttgt tcattatttt gctcaggagc tgcggcttgt gcctgctgag aagcctgttc
     7021 agctgctgct tgctgtcgcg cctgttctgc ttgttgctgt gcgattgctt cttgcctcgc
     7081 ctgttctgct tgttgctgtg cgattgcttc ttgttgcgct tgttctgctt gttgtcgtgc
     7141 aatttcttct tgctttttcc gttcttcttc tgcttctttt gctttgattg tggaatcaat
     7201 tgcagcaaga cggcttgtca actcttgttt gttttcaggt agcgcttgga tagctgctga
     7261 agcagttgca tagttttctt ttgtaggatt tgcttcagct tgtgacaaag cagattccgc
     7321 ttctgtcact tttttttcta gtgcagcttt ttccgctttt tgtttctctt cttctttttt
     7381 cttttcaatt tgttctttcg atgaatctgc cgtactttcg gagatcgtgg tactgctgct
     7441 ggaagcctga gctgaacttg tgctttgagt agtagacgta gtagcaccac acccactcaa
     7501 caaacttcct gcgaccaaaa acaacgcaac taattttttc actaaatttc ctccttaagt
     7561 aaaaaacaac cgttttcaat aaatgtcctt ttcaattgtt tacttacttg ttataataat
     7621 tgtgttatga atagtgattg aggaaactta atctaatccc gtgttgatac acgggatttt
     7681 t
//
LOCUS       DAZAOG010000126         7646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000126 DAZAOG010000000
VERSION     DAZAOG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.126"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACHDLD_002214"
     CDS             complement(59..787)
                     /locus_tag="ACHDLD_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3363897.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACHDLD_002215"
     CDS             complement(800..2080)
                     /locus_tag="ACHDLD_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3363898.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACHDLD_002216"
     CDS             complement(2221..2823)
                     /locus_tag="ACHDLD_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN3363899.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACHDLD_002217"
     CDS             3068..3274
                     /locus_tag="ACHDLD_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363900.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACHDLD_002218"
     CDS             3586..4305
                     /locus_tag="ACHDLD_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3363901.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACHDLD_002219"
     CDS             complement(4386..5069)
                     /locus_tag="ACHDLD_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN3363902.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACHDLD_002220"
     CDS             5287..7473
                     /locus_tag="ACHDLD_002220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN3363903.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2735 a         1291 c         1439 g         2181 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
     7621 aagaattttg tcacacctcc cttatt
//
LOCUS       DAZAOG010000127         7534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000127 DAZAOG010000000
VERSION     DAZAOG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.127"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHDLD_002221"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHDLD_002221"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN3363904.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACHDLD_002222"
     CDS             complement(2032..3285)
                     /locus_tag="ACHDLD_002222"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN3363905.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACHDLD_002223"
     CDS             complement(3291..4214)
                     /locus_tag="ACHDLD_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN3363906.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACHDLD_002224"
     CDS             complement(4435..6927)
                     /locus_tag="ACHDLD_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN3363907.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACHDLD_002225"
     CDS             complement(6934..7401)
                     /locus_tag="ACHDLD_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN3363908.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZAOG010000128         7412 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000128 DAZAOG010000000
VERSION     DAZAOG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.128"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..1633)
                     /gene="cls"
                     /locus_tag="ACHDLD_002226"
     CDS             complement(185..1633)
                     /gene="cls"
                     /locus_tag="ACHDLD_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN3363909.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1785..2669)
                     /gene="ftsX"
                     /locus_tag="ACHDLD_002227"
     CDS             complement(1785..2669)
                     /gene="ftsX"
                     /locus_tag="ACHDLD_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN3363910.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="ACHDLD_002228"
     CDS             complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="ACHDLD_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN3363911.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3453..4554)
                     /gene="prfB"
                     /locus_tag="ACHDLD_002229"
     CDS             complement(join(3453..4481,4483..4554))
                     /gene="prfB"
                     /locus_tag="ACHDLD_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN3363912.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4680..7214)
                     /gene="secA"
                     /locus_tag="ACHDLD_002230"
     CDS             complement(4680..7214)
                     /gene="secA"
                     /locus_tag="ACHDLD_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN3363913.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1987 a         1645 c         1291 g         2489 t
ORIGIN      
        1 gtgtctcttt taatagcata tattagctta tttcttgctt tccccaaaaa acaagagata
       61 ccccaaagtt aatttcgctc atgcgagacg ctttctccat ttctgacgta ctctcagaaa
      121 tggccccgtt agaaaaggac acgagatctc catctgttga tggttctcat gtccttttta
      181 tgttttaaag gagcggcgaa atcaatcttg ctaatccttc tttcatcttg atgattattc
      241 ctcggttttc atacatttct tttgtgagat gcgtagaaat tttcgagtct tcaaaaaaag
      301 cttttcttac acgtgaagcc attcgttcat cataaaccag tgtattgacc tcaaagtcca
      361 agcgaaatga acgaacatcg atatttgctg agccaactga aacaatgcca ccatcgataa
      421 tcatcgtctt tgcatgaata aatccattct catatgtttc gatccttgct ccatactcga
      481 ttagctctgc agcgaaagaa tacgttgccc agtaaacgag aagatgatct ggtttgttag
      541 gtatttggat atgaactttt acgcctgaaa gaagagccaa cttcagtgct tcatgaatcg
      601 atccatccgg tatgtaataa ggcgtttgga tcaatatctc ccgtttggct aacgaaatca
      661 ttttcaaata agtcattttg atttgttcat gttcggtatc tggtccgctt gtcacgattt
      721 gaacagctat atcaccagta gagtgaatgg acggaaaaaa tttgggagaa tagcctactt
      781 cttttgtatg ctgagaattc cagtccatca aaaaacggtt ttgcaaggta tacaccgctt
      841 ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa tttctttacc aatcctagat
      901 attcatttcc aacattgaac ccacctgtat atccgatttt cccatcaata actacgattt
      961 tacgatgatt gcgataattg atccgaggat taatatacgg aacaaataga ggaaagaaaa
     1021 atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa gctggaagaa acttgtgtgg
     1081 aaccccatgc atcaagaagg actctgactt ttacccctcg tcttgccgcc tctgtcaatg
     1141 catctcgaac ttcttcacct aatgcatcac tgcgataaat ataatactgg aagtggatat
     1201 ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa tttttcacga ccatctgtaa
     1261 ataaagtgat ttcattttta gtcgtataga gcgtactttc aaataccgtg agcatataga
     1321 tcaattgttt cacatctact tgtccagtag gcggatgagg ataaagccct cgcatcaatg
     1381 cctgtttgtc ttcttcaatt tccaggttct taccgatttt cccttggatc ttcaaatcaa
     1441 agattttctc tttggaaatc cctcttccta aaaaaatata taaaataaat cctacaactg
     1501 gaatgaacat caaaacgagc acccatgccc atgtttgagc agtttccttt ctttctcgaa
     1561 aaacgatgat aaaggaaagc aaaatattca taatcaaaag gatcgtaaaa aaattgtcaa
     1621 cgataatttt cacggcttca cctcttcttc taccaactta agaataccca agttgttttt
     1681 taaagtaaag ggaaaaaagc cgcttccttc tattctccta gccaataaaa aaagaaagct
     1741 ggaacgaaac tgtccaactt tctcatttta tttatattgt ggtattagaa tttcaagaaa
     1801 cgtctcatag aaatgatcga gccaagtgaa ccaatgatca caccaatgcc gaccattaaa
     1861 agattgattt tccaaacgat attttgtgga tgaatcaatg aataatggga acgtaataat
     1921 gatggattga tcaattggaa aaaccatgca taaccaaatg tgatgatcaa aatcggaaga
     1981 atcgcaccga tcagaccgat ccagccacct tcaaggaaaa acggccagcg aatatatcca
     2041 ttttttgcac cgactaaacg catgatttga atttcacgct gtctagacaa aatcgtgatc
     2101 cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca gcaatgccgc tgcaactaat
     2161 ccccatgtcc gaactgcacc agcgatttgg aaaatccgat ctgaggaagc tccaccgtaa
     2221 tcagaacgag aaacattctt caaatctgct gcttcttctg ctacttgttt tgtataagaa
     2281 ggatcagtag cacttactac atacacatca tataacgggt tattgtcacc ttcaaacaat
     2341 ttccaggaat cacccatttc gttttggatt tttttcaatt cctgttcttt gcttgaaaat
     2401 tcaacttttt taacatgatc taagtcattc aattcttttt ctagcttttt catctcttcg
     2461 tcttttgtac cgatatctac gaatacagag acatcaacgt ttccttctat atcttgagct
     2521 aatttagttg cgttcatgat aactgccatg aatacaccga ctaaagtcaa tgtgatcgtt
     2581 accgcactga ctgaagcaac tgtcatccag ccgttacgtt tcaaactctt gatactttct
     2641 aataaatggc ggaaaaaggt tctaatcatc gtatccatat tccccttccg cttgatctcg
     2701 aataatgcgg ccgttttcga tagcaattac tcggtgccga atcgtattta cgatcgtact
     2761 gttatgtgta gccatcacga cagtagttcc ttgggcattg atccgatcta ataatttcat
     2821 gatttcccat gagttttctg gatccagatt tcctgtcggt tcatccgcga taagtacttt
     2881 aggcgtattc actattgccc gagcaataga gacacgttgc tgttccccac cggataattc
     2941 gctaggaaag actcgaactt tgtgtttcaa accaacaagg tccagcactt ccattactcg
     3001 ttttttgata tcccgaggtt ttctgccgat aacttgcata gcgtacgcaa cattttcata
     3061 tactgttttt cttggtaata atttatagtc ttggaacacc acgccgattt ctctgcgtag
     3121 ataaggaact tcgcggtttt tgattgctat tagatcatga cctgcgacgt tcaaagttcc
     3181 ttttgaagct ttttcttctc gatacatcaa tttgatgaat gtagatttac cggctccaga
     3241 cggaccaacc acatagacaa actctccttg ttcgatgaca attgagagat tgcggatagc
     3301 ggtcgtacca tttgagtact tcttcattac atccttcatt tcaatcatgg cgttctctcc
     3361 atatcttttt cagtctttag ctattatagc atggcaatgt ttcaacatgc ttgcaaaatc
     3421 ttacaatttt atttcaattt aacaatgggt tcttattgta gatgcatttt taaatagccg
     3481 tcaataaata aatcaaggtc accgtccata actgcttgga catttcctgt ctctacgttt
     3541 gtccgatggt cttttaccat agaatatgga tggaaaacat aagaacggat ctgtgaaccc
     3601 cagccgattt ctttttgttc tcctcgaagt gcagctgctt cttgttcttt cttttccact
     3661 tctaattgat aaagcttagc ttttaacatc cccatcgctt gctcacggtt tttcaattgt
     3721 gaacgttgtg cctgactagc tactactgtt cctgtaggca agtgagtgat ccgtacagca
     3781 gattctgttt tattgatgtg ctgaccacct gcaccacttg ctcggtacgt atcgattttc
     3841 aaatcatctg gattgatttc tatatcgatc gtttcatcaa gctctggcat cacatctacc
     3901 gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag gcgagatacg tacaaggcga
     3961 tgaacacctt tttctgattt cagataacca aatgcgttgt gtcctttgat cagtagggtc
     4021 acactcttga ttcctgcttc atcacctgct tgataatcta gtgtctctac ttgataccca
     4081 tgctgctctg cccagcgtgt atacatacgt aaaagcatac tgccccaatc ttgggactcc
     4141 gttccgccag cccctggatg caattcaacg atggcgttgt ttcgatcata aggaccatct
     4201 aacaacatag ataattcata tgtcttcatt ttttcatcta gcgcattgat acgtgtttct
     4261 gcttcttctt gcatggctgc atcaggttct tcctgcaaca tctccaccat caattcgatt
     4321 tcttccagtt cttcagcaag agaatggaat tgatcataag tttctttatt tgcattgttt
     4381 tcactgatca ctttttgagc agcttcgcta tcatcccaaa aaccaggttc agccattttt
     4441 gcttctgctt ccgcaatgtc ttcttccagc tggtctaagt caaagagacc tcctgaagct
     4501 tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt tcggatattt ccatgttttt
     4561 tccttctttc actatacttt tttcattata tctagtctaa cagaatcagc aaataacaga
     4621 aagaaggtga gacgaattct taaaaaaaga atttgtctca ccaatcctgc tgtcagaaat
     4681 tagtttgcat tttttccatg acagttctta tattttttcc cgcttccaca aggacaagga
     4741 tcatttcttc cgaccttgtt cacatggatt ggctgttttt tcgcgctagt attactattt
     4801 tctttggcat ctccgtcttc tgagtgagaa gctgttcctt gagaaacctg ttcacgctgt
     4861 acgttttgac ggatctcagc tttcataaag attcgagtga cttcatactc gatggcgcct
     4921 accatatctt caaacatctt atagccttct gtttgatatt cgactaatgg attgttctgt
     4981 ccataagcac gtaagccgat tgactgacgt aattgatcca ttgcatcaat atggtctgtc
     5041 catttagagt ccacaacacg caagatcacg actttttcaa attccaacag ttgctctgga
     5101 ccatttagtt gagccgcttt ggtattaaat acttcttgtg ctcgttcgtt tagatactct
     5161 ttgatctcct gagctgtttt tccttcgata tctgcaagag taatactatc ttcatggaca
     5221 agagtactgc cggcaaaatc gatgatactt tccaaattcc aattttcttt ctctaattgt
     5281 gtatgcgcat ctactacacg actgattgtc cgtttgacca tgttcattag tggcttggat
     5341 aagctttttt cttccatgat tacttcttgt cgctgtttat agattacttc acgttgttca
     5401 cgcatcacat catcgtattg taagacgttt ttacgtgtgt catagttatt tccttctaca
     5461 cgtttttgtg cagattctac ttgtttcgtc agcatcttgc tttggataac agcatcttcc
     5521 tcaccgatct tcatccgatc taagaaagat ttgattcgtt ctgaaccgaa tcgtttcatc
     5581 aaatcatctt ccaatgacaa gtagaactgt gacataccag ggtctccttg acgtcctgca
     5641 cgtcctcgaa gctggttatc gatacgacga gattcatgtc gttctgtccc gataactgct
     5701 agaccaccaa gttctcttac cccaagtcct aacttgatat ctgttccccg accagccatg
     5761 ttggtagcaa tcgtcacagc acctttttga ccagcattca tgatgatctc tgcttcttta
     5821 aagtgatttt tcgcattcag cacttcatga ggaatacgtt cttgattcag catatctgat
     5881 aataactcag atgtttcaac cgcaacagtt ccgactaaca caggttgacc tttgcggtag
     5941 cgatctttga tatcttgcac gacagcgtgg aacttgcttt gtaatgttgg gtaaagcaaa
     6001 tctggacgat catcacggat aaccggacga tttgtcggaa tctggtatac ttggatgtta
     6061 taaatttctc tgaattcttc ttcttctgtt ttagctgtac cagtcattcc cgcaagtttc
     6121 ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca tggttttcgt ttcgtcttcg
     6181 atctctacgc cttctttagc ttcgattgct tggtgaagtc catctgaata acggcgtcca
     6241 tccatgatcc gaccagtgaa ctggtcaaca atcagtactt ttccgtcttg gacaacataa
     6301 tcgatatcta aaagcatgat atagttggct ctcaatgctt gatccaagtg atgggtcaat
     6361 gcagtatttt caatgtcgta taagttatcc aaaccgaatg tttcttctgc tttttcgatc
     6421 cctgcttcgg ttaaactgat tgtttttgat tgtacatcga ttttataatc ttcttcttct
     6481 ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag ctgtggattt ttctgcttgc
     6541 cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga ttgagtcaac ttcatcgaca
     6601 atggcataat tcagcggacg ttgtaccatt tgatggcggt aaacgaccat attgtctctc
     6661 aagtagtcaa atcctaattc attattcgta ctataagtga tatcacaatt ataagcctcg
     6721 cgtttttcat ccgaagattt tgagttgata ttcaacccca ctgttaatcc taagaaatta
     6781 taaagttcgc ccatttccgt cgagtcacgg gttgctaaat attcattgac ggtaactacg
     6841 tgtacccctt cgccagtcag agcatttaga tatactggca tcgttgcggt caaggtttta
     6901 ccttcaccag tacgcatttc agggatattt ccatcgtgaa gcacgattcc acccatcaat
     6961 tgtacgtggt gtggatataa ccccaataca cgttttgctg cttctcggac gactgcaaaa
     7021 gcttccggaa gtaattgatc taatgtttct cctttttggt atctgccctt gaattcagca
     7081 gtttttgcct gtaattgttc atcagttaat gctgccattt ggtcagcgta tgcttcaact
     7141 ttttttgcaa tgctatctaa acgttttagt tctttcttat cgttttcgat aagtgaacga
     7201 atcaaattgg ccatgcgttg tgtaatctcc ttttccttct gtgtattatc cagactatgt
     7261 agactttact tttcatttta tcattagtta tagataattg aaatatctta agcgtattaa
     7321 agtaagaact tcataattta ttcatatttc atctaaaaga ggaggaaact caaaaaaact
     7381 tgcggcagct gtcagtagct gctgcaagtt tt
//
LOCUS       DAZAOG010000129         7397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000129 DAZAOG010000000
VERSION     DAZAOG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.129"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..1846
                     /locus_tag="ACHDLD_002231"
     CDS             215..1846
                     /locus_tag="ACHDLD_002231"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN3363914.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1849..2928
                     /gene="uxuA"
                     /locus_tag="ACHDLD_002232"
     CDS             1849..2928
                     /gene="uxuA"
                     /locus_tag="ACHDLD_002232"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3363915.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            3002..4429
                     /gene="uxaC"
                     /locus_tag="ACHDLD_002233"
     CDS             3002..4429
                     /gene="uxaC"
                     /locus_tag="ACHDLD_002233"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN3363916.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4513..5160
                     /locus_tag="ACHDLD_002234"
     CDS             4513..5160
                     /locus_tag="ACHDLD_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN3363917.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5289..6665
                     /locus_tag="ACHDLD_002235"
     CDS             5289..6665
                     /locus_tag="ACHDLD_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3363918.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6676..>7397
                     /locus_tag="ACHDLD_002236"
     CDS             6676..>7397
                     /locus_tag="ACHDLD_002236"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3363919.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFK"
BASE COUNT         2575 a          970 c         1515 g         2337 t
ORIGIN      
        1 aaaaaatact caaataagat caaagagaaa ttaagcttta atttaagtta gttctaaaaa
       61 atatatattg gtgtgctttt actaaaaaaa gacacaataa ttctgacatg gtagaaaaaa
      121 gaatattttt agttttttct tattgacagc gattacattc gattttatac tacaattgta
      181 acggaaacta acatatcagt atggaggggt aaatatggtt ttgattaaag actggcaaga
      241 ttctatcgaa ggattgaaca aaatgcaagt tcaactaccg tattttaatg tcgaagcggt
      301 caaacaagaa ggtcagaaac atccagtgtg gcttcatttt ggtggaggaa atctttatcg
      361 aggatttcat gcagaaattg ctcaatgctt agcagatcaa aatgaattag aatccggagt
      421 agttgtgtgt gagacatttg atgaagaagt agtagaaaaa gcttatcatg actacaacaa
      481 tgatattttg gagattgtta tgggtgaaga tggtgaactc aaaaaaagac ttttagcagt
      541 aacagctgat agtttatatt gcaatccaaa gaatataacg gactatcaaa gagtggttaa
      601 atattttgaa gatcctacat tacagtttac aacgtttact attactgaaa aaggatacag
      661 tttaaaagat tctaaaggac aatttttttc ggtagtggaa gcagatataa aatctggacc
      721 taccaatcct caacatacga tgagtattgt gacatccttg ttgtatagca ggtttaatgc
      781 tggagagtta ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt
      841 tcaagatagc atttttatga ttgcagaagc ttggcaaaaa aaaggatacg tttctgaaaa
      901 atttattgct tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatag
      961 aattacgcca aatccttcgg aaagtgtcaa aatggattta ttgaaggcgg gtttcgaaga
     1021 cttagatata attcatactg cgaaaaatac caatatcgct gcttttacca atactgaaaa
     1081 agtccattat ctagtcattg aagatagttt tccaaatggt cgacctaaac tggaagcggc
     1141 tggtgtgatt atgacagata gagaaacagt tgataaagca gatatcatga aggttacgac
     1201 atgtttaaat ccgctccata cggcattagc aataacaggt tgtttgttgg ggtataaatc
     1261 aattcaggca gaaatgcaag atgatgattt ggttggctta attaaaggga taggttatgt
     1321 agaaggctta ccagtagttg aaagtccagg cgttattgat ccaaaacaat ttattgatga
     1381 agtaatcaat aaacgtttgc ctaatcccta tattccagat acaccgcaac gaattgcaac
     1441 agatacttct caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa
     1501 agacggaaat gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta
     1561 tttattggca gtcgatgatc aaggaaaaaa ttttgtgcca agtcctgatc ctttattggt
     1621 agagttgcag gaaaaattct cagtattgaa attaggtgaa caagatcctg caaagattca
     1681 ttgtgcggta caggaaatct tagaaaataa ggaaatattt ggtttagatt tatatcaagt
     1741 tgagatagga aagaaaattg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt
     1801 tcgtaaaaca atcaataaat atataacaga agttggagga aagtaactat ggaaatgact
     1861 tttagatggt atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca
     1921 gcaatgaaag gaattgttgg aacattaatg gatataccag ccggagaggt ctggccgaga
     1981 gaacgaatta aagctttaaa agatgaagtc gaagccaatg gccttacctt gaaagttatt
     2041 gagagtgtta atgttcatga tgatatcaaa attggtttgc caacacgaga tcaatacatc
     2101 gagaattata aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat
     2161 aattttatgc cagtctttga ttgggtaaaa tcagatttgg attatcattt agatgatggg
     2221 tcttctacgt tagcctatat ttcaaaaaat attcctactg atccagagga aattattaga
     2281 aaagtagaaa attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca
     2341 caaatcaagc aattattaga agcctataaa gaagttgatg aagcgaaatt gcgagagaac
     2401 tttgcatatt ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca
     2461 gttcatccag atgatccacc atattctatt ttcggattac ctcgtattgt taaaaatcgt
     2521 gacgatttag aatggttgtg taatgtagtg gattctcctt ctaatggtat tacgttatgt
     2581 actggaagta ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt
     2641 caacgggatc ggattccttt tgcacatgtt cgtaatatca aatatttaag tgataatgga
     2701 ctcgattttt atgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata
     2761 ttaaaaacaa tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg
     2821 atttggggag aaaccggtag acctggttat gggttatatg accgagctct aggtgcagtt
     2881 tatttaaatg gtatttggga agcgcttgaa aagggggcga gtaaataatt atataatgga
     2941 gtctatttag aagaaaaatt ttctcttcta aaaactaagg gaaggaaagg agtgcttaaa
     3001 tatgttttta gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa
     3061 atttgcagaa caccaaccaa tcatcgatta tcattgtcat ttagacccag catgtttgct
     3121 agaaaataag caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga
     3181 tcattataaa tggcgattga tgcgagctaa tgggatagaa gaagagttga tcagtggtgc
     3241 aggtaatgat tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa
     3301 tcctattttt gaatggtctc atttggaatt acaacgttac tttgatattg atttgacaat
     3361 ttctgagaag aatgcaccag aaatttggcg acgtgccaac gaaaaaatcc aaacggattc
     3421 ctatcgccca aaagaattgc tgaagatgat ggatgtgaaa gccatttgta caacggatga
     3481 tcctacagat gacttgcgat atcatactga gctttctaaa caggataatt ctttcaaggt
     3541 attacctact ttccgtccag ataaggtctg gagtatcaaa tcatcaggat tttcgaacta
     3601 tattttaaaa cttagtcaag cagcgggtat accaatcaaa aactttaatg ggttgaagca
     3661 ggcattgagc caacgagtgg attattttca tcaaattggc tgtcgattag cagatcacgg
     3721 tttgaatact tatttttatg aaatagcaga tgaaaaaata ctcgacaatc tgttaataaa
     3781 agctttagca ggaaaaacgg atttccattt atcagaaatt gcacaatttg ttacaggtat
     3841 tcaattacat ttgatgtctg agtatacaag atatggttgg acgatgcaaa tgcatttaaa
     3901 tgctttaaga aataattcaa gtatattaaa ggaacaaatt ggagtagatg tcggtggtga
     3961 ttcgatgggg gatcaagcta gtttgacaca aaatgttgta gcattgttcg acactgctga
     4021 aaagagtggg tgcttaccaa aaacaatttt atattcatta aatccaaccg attggcttcc
     4081 actagcaaca gcaatgcaaa gcttccaagg gggtatgatt caaaaaatcc aattgggatg
     4141 tgcttggtgg ttcaatgata cagctgaagg aatgcgtcaa cagttgacta cgatggcgca
     4201 acaaagtttg ttgtcgaatt ttacaggtat gttgactgat tctcgaagtt ttctaagcta
     4261 tcctcgccat gagtatttcc gtcgagtatt atgtcaatta ctaagcgagt gggttaaagc
     4321 aggtcgaatt ccagatgatg aaatgtatct tagtcagata gtggagaata tttgttatcg
     4381 taatgcgtat aattatttta atttttttga aatcgaaagt agaaaataat acaaaaaata
     4441 aaagcttaaa tttatttatt gtaagttagt acaactagct agagtatgta tccataaaag
     4501 aaggagatag atatgaaaaa agtagaaatt ttaagtaagt tagaagcggc tggagtcatt
     4561 gcggttgtac gtggaaaaaa taaagatgag gccattaaag caataaatgc aattactgcg
     4621 ggtggaatta aagggattga actaactttt acagttccga atgctgaaga aataatcaaa
     4681 gaaattgcta aaaaatatgc tgatcaaaaa gatgtaataa ttggagccgg tacagtttta
     4741 gatgcgttta ctgctcgtat tgcaattatg gctggagcag aatatatcgt aagtccatct
     4801 tttgatcgac aaacagcaga gatttgtaat ttatatcaaa ttccttattt accaggttgt
     4861 atgacaatta ctgagataaa ggaggcactt accgctggag tagaaatcat taaactattt
     4921 ccaggttctg cttttggtcc atctgttgta aaaacgtatc aagctccatt accatatgta
     4981 aatataatgc ctactggcgg agtaagtcta gataacttag aagagtggtt tgaagcaggc
     5041 gttgttgcag ttggtgtagg tgggaatttg ttggctcctg ttgaaaaagg tgattttgaa
     5101 gaagtaactc gaatagctag taaatatata gataagtttt atgagataaa gaattattag
     5161 gagaaaaaat aaataatact ataaaatgta aaaaataaat actaactctt tatacttcag
     5221 agtatagtac gtaagttggc agttcaacag aatgaaaacg cttaacaata ggaaggagcg
     5281 caaattatat ggaaagaaaa tttggtctaa gagacaaaat aggatatatg tttggagatt
     5341 ttgggaatga tttcactttc atttttgcta gttctttttt aatgattttt tatacaaaag
     5401 tattggggat aagcggagct accgtaggaa cattattttt agtagcaaga tttattgatg
     5461 cagttacaga cataacaatg gggcgtattg tggatggatc aaaaccatct aaaactggca
     5521 aatttaaacc gtggatttta cggatgagtg gtccagtcgc tttagcaagt tttttaatgt
     5581 atcaaacatc aatggcatca gcttctatga gtattaaagt tatatatatg tatgtaacat
     5641 atatactatg gggaagtatt ttttatacat ctattaatat tccttacggt tcaatggcat
     5701 cagctatcac ttctgttgca gaagaaagga cagcgttatc aacatttcgt agtataggag
     5761 ctaccttagc aggtatgttc attggcgtgt taacaccaat ttttatttat acaagagata
     5821 gttctggagc tcaaattgta cgtggtggat caacttttac aattgtggca ggagtatttt
     5881 cagtgtgtgc agttatttgt tacgtaatat gttataaatt aactgtagaa cgggttaaaa
     5941 gtgatgaatc gcagttaaaa gtttcattgt tgaaatcttt aaaaggatta gctaaaaata
     6001 gagcgttgct ttctataata gctgcagcta tttttttgtt gcttgctcag cttttaacaa
     6061 tgagtatgaa caattatgta tttccagatt attataataa tgcacaggga atagttttaa
     6121 tgaactttat caatcctatt ttgatgttgg tacttgtttc acctgtaagt ttaattttca
     6181 gtaaaaaata cggtaaaaaa gaattggcgt cagtagggat gttcttctct gctattgtat
     6241 atttactttt atttttgata aagcctagta atatgtatgt attcttagtt cttacttctg
     6301 ttggatatat ggggttaggt atttttaata cagtcatttg ggcgaatatt actgatgtaa
     6361 tcgatgatca agaagtgaaa tctggccaaa gagaagatgg aactatttat gcagtatatt
     6421 cttttgcccg aaaaattgga caagctttag ctggcggtgt cggaggttgg actctttcta
     6481 taatagggta tgattcgtta gcaaaagtac aaagtggaga agttttacaa aagctgtata
     6541 actcttcaac gttgattcca gcagtttgtt tctttgttgt aggattgatt ttatttttcg
     6601 tatatccatt atctaaagaa aaagtattag aaaattataa tttgctaaat caaaaacata
     6661 actaggagaa aaaacatgaa cagttggata ttttgtattg attcagatgg ttgtgtcatg
     6721 gatacaatga cttataaaca caatctcttt ttcgggccaa ttgctgcaga gatttttgat
     6781 gttcatcata gggaaaaatt cctacaggat tggaataata ttaatttata ttcagagact
     6841 cgtggaataa acagattttt aggtttagtt aaaacattag aaaaaaatga tgtaccatcg
     6901 attaatcgat taaagcactg ggtatctgta acaaaagaat tgtctaatcc atctttgaag
     6961 agggaaatag aatactctca gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa
     7021 gtcaatcgac agattagtca agctaaagga ttagataaac cctttttaag tgctagagat
     7081 actataaaag ctctgaaaaa atatgggaag ataattattg taagctcagc taataaggaa
     7141 gctattcaag aagaatggga aagacatgaa ttattatcct tagttgatga actgtgttgt
     7201 caagataaag gaaaaaaaga agacattatt cgaagtgtaa ttgaaaatgg ttgtgatttg
     7261 gataagatat taatgattgg tgattctcct ggtgatctag aagctgcaaa taagaataaa
     7321 gttttctttt atcctatttt ggttaataaa gaaaaagaat catgggaaaa cttaagaact
     7381 gatattttat ttaaatg
//
LOCUS       DAZAOG010000130         7281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000130 DAZAOG010000000
VERSION     DAZAOG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.130"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..2675
                     /gene="clpB"
                     /locus_tag="ACHDLD_002237"
     CDS             66..2675
                     /gene="clpB"
                     /locus_tag="ACHDLD_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN3363920.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2826..3512)
                     /locus_tag="ACHDLD_002238"
     CDS             complement(2826..3512)
                     /locus_tag="ACHDLD_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363921.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3490..3984)
                     /locus_tag="ACHDLD_002239"
     CDS             complement(3490..3984)
                     /locus_tag="ACHDLD_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN3363922.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4004..5224)
                     /locus_tag="ACHDLD_002240"
     CDS             complement(4004..5224)
                     /locus_tag="ACHDLD_002240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3363923.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5371..7206
                     /gene="lepA"
                     /locus_tag="ACHDLD_002241"
     CDS             5371..7206
                     /gene="lepA"
                     /locus_tag="ACHDLD_002241"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN3363924.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2393 a         1393 c         1497 g         1998 t
ORIGIN      
        1 ataatagttt ttgtaaagat caaagaaata agtgatctta cctttaatta ggagggtata
       61 cctgtatgaa tattgagaaa atgactacga cgcttcaaga agcgattgct gaagctcaac
      121 agattgcggt cactcgaaaa catcaagata tcgacattgc ccatgtatgg aaaatctttt
      181 tacaatctaa tcattttggt cgaaatttct atacggatgc cggattggat gtcgaatctt
      241 ttgaacatga aattgatcgt ctactggatg agtatccagt tgtttctggt ggaaacgtcc
      301 aatatggaca aaacctcagc cagaatttct ttcgattatt gaatgaagca gaccaaatac
      361 gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt tattttagcg ttgatgaaat
      421 taaaaaatta tccgttaact gtttatttga ataaaaacgg attatctgaa aaagaattgc
      481 gtaaaaacat cgaagagatg agaggaggag aaagagtgac ctcacaaaac caagaagaac
      541 aatacaaagc attagaaaaa tacggcgttg atttagtaca gcaagtcaaa agcggaaaaa
      601 tggatccaat catcggtcgt gatgaagaaa tccgtgatgt catccgtatt ctttcacgaa
      661 aaacaaaaaa taatcctgtt ctgataggag aacccggtgt agggaaaacg gcaatcgttg
      721 aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga aaatttaaaa gataaaacga
      781 ttttttcatt agatatgggt gcattgatcg ctggagcaaa attccgtggt gaatttgaag
      841 aaagactaaa agcagtcttg aaagaagtca aaaaaagtga tggacgtatc ctcttgttca
      901 tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga aggaagtatg gatgccggaa
      961 atctgttgaa accaatgctt gctcgcggtg aattacattt gatcggtgcc acaactttag
     1021 atgagtaccg tcaatatatg gaaaaagaca aagcgttgga acgtcgcttt caaaaggtat
     1081 tagtcaaaga accaacagtg gaagatacga tcagtattct gcgtggatta aaagaacgtt
     1141 ttgagatcca ccatggtgtt aacatccatg acaacgcatt agtagcagca gctactttat
     1201 ctgaccgtta tattactgat cggttcttac cggacaaagc aatcgatttg atcgatgaag
     1261 ccagtgcaac gattcgggta gaaatgaact ctatgccaac ggaattagat caagttacta
     1321 gacgtttgat gcagttggaa atcgaagaag ctgcactgaa aaaagaatca gatgatgcaa
     1381 gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga acttcgtgaa gaagcaaatg
     1441 cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa ttctgtttca gcaaaacgtg
     1501 ctgaaatcga taaagcaaaa cacgaattag aagatgcaga aaataactat gatttagaac
     1561 gcgctgctgt attgcgtcat ggtacgatac ctcaattaga aaaagaattg aaggaattag
     1621 aagctaaagc aaaagacagc gagattaaga tggttcaaga gtccgttacc gaaaatgaga
     1681 ttgctcaagt cgttggcaga ttaacgggta tccccgtaac gaaacttgta gaaggtgaac
     1741 gagaaaaact aattaaattg aatgaaacac ttcataaacg agtgattggt caagatgaag
     1801 cagttgatgc ggtcagtgac gcggtaattc gttcacgggc tggattgcaa gacccaaatc
     1861 gtcctctagg ttctttcctt ttcctaggac caactggtgt cggaaaaacc gaattagcga
     1921 aagcgttagc tgagaacttg tttgactcag aagatcatat ggtacggatc gatatgagtg
     1981 aatatatgga aaaacatgct gtgtctcgtc tagtcggagc tcctccaggc tatgtaggtt
     2041 acgaagaagg tggtcaatta acggaagctg tcagacgaaa tccttataca atcgtcttgt
     2101 tggatgaaat cgaaaaagcg catcccgatg tcttcaatat cttgttacaa gtcctagatg
     2161 acggacgctt gactgattca aaagggcgag tagtagattt caaaaatacg gttctgatca
     2221 tgaccagcaa tatcggttca cagttactgc tggaaggcgt gactgctgat ggaacgattc
     2281 cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg aaacttcaaa ccagaattct
     2341 tgaaccgaat cgacgacacg attttattta caccattgag cctagacaac gtgaaaggaa
     2401 tcgttgacaa aatggtggcg caattggcac aacgtctgga acatcaagaa atcctgctga
     2461 caatcagtga tgaagccaaa acgtggattg ctgaaaatgc ttatgaacca gcatatggtg
     2521 caagaccatt gaaacgattt atcactaagg aagtagaaac accactagct aaagaaatcg
     2581 ttgcaggaca tgttatgcct aaatcaaaag taacgattac tttacttgat ggacaacttc
     2641 atttcaaaac agaagagcta gaagaaatcg tctaatcttc cattgagact ataaaagaaa
     2701 aaaggggtct gacacaacta gtgtcagacc tccttttttc aaataaacag tgttccagaa
     2761 gtagtttctt cgaaaaataa gccgaaattt cttgaagctg cccgcttctg tcacaacctc
     2821 ttttattagg tatttttcac tttttctaat ttataacttt caaagtaacg ctcatctttt
     2881 ttactttgag accatgcaaa caatcccatt aaggcagcac ctatcgtatt tgtcagcaag
     2941 tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca gtgtgccatc agaagcagca
     3001 aaacgttgaa gattcatgcc taagaactgg tcgcataaga attcccagaa ctcccaaaac
     3061 acgccgcaaa gtccagcaaa cgcaaagccg aataagagga acaaccaagg actggttttt
     3121 gatatctcag catctttcat caagtaacca atcaatccat agccaagagc tgtcaggacc
     3181 attggactca ccgcatgtaa gattttgtcc caaaaagaga tgatactgat caaatgcatc
     3241 cctgtgccta aaaagacaga gatgaacaag aaaaaccagt aaaacaaaac gattgctgct
     3301 ggtattttaa gtttgaaaac tttcaaaatg acttgtggca tgaaaatcaa tgcaatccct
     3361 aataaagatt ccacgataaa tgtgattcct tgagaagtcg aatatttttg tgtgataaat
     3421 tcataaaaat tatagcagac aacgaataca ccaaaagcaa gtaaagctat ttgcaccttt
     3481 ttgatttctt taatcactga aagtccctcc atctaaaaat ctttttactt ctgaaagcaa
     3541 ctcaatttct gaagaaacca cttttccgtt caatttgatc aaacctattg tatataagtt
     3601 caggtagtga aattgatttt cagctgtttc ttgtagtgca gcgatttttt tcgggtgggt
     3661 tccgccttgc tgtcgagtat cagtatataa gccgatcact gggatgcctt tcgcataagc
     3721 cacaccgatt tccgatgcta ccccggcatc gatcgtcagc ccatctagta aagcaatcat
     3781 taaatcacta gccaacactt tttctgtgtc agcaagagca atcattcgac tgtctgcata
     3841 agcagttttg tcattgattg cttcattttc ttgaggaaga tagatcgaaa gctcagaaga
     3901 gagctctctg atttctttaa ctaactgttg gttatataac aaatcacttt tagcaaacat
     3961 tggtgcggca aaataaattt tcatctaaac atcatccttt ttatcaaaaa taatcgctaa
     4021 agtaagcttc catgtttgga atgatacgct ctagtgactt cagtgtttct tcatctgttt
     4081 ctaaccgttc gattcttgcc aaataagaag cacggcgata attcatcatc aagcaagtca
     4141 atgtttggat atccagtcga acggattttc ctataggttc atttaagaca cggacttgtc
     4201 cctgttcatc ccatgttaaa gcgaagacac cattattcca aggagcgaca ggatcttcaa
     4261 tgatgaaatg aaaagcatca gctgttccaa caaacggaaa ccgttgaagg aattctttta
     4321 catcaacgat ccgagccata aaatatggtt cgatctgctc tttgatctga ctatcttcaa
     4381 tcaaaaatgc taaaggctca tttttataaa tatctccatg tacccagtag atcatggaaa
     4441 aatgtgcgga aataaagttc cataagccat ttcgagcttc ttgatcaaga tagaacattt
     4501 cttttacgtg aaatatttct tcagctaccc agtagaacag aacacctctt ggttcatggt
     4561 tgctatcgta gtagacagcg gctgtgcgct catcttcatt ttcgaaacgc caatactctt
     4621 cccaatgaaa atcactgcgg aataaagcac catggttttg ttgggcaaat ttgtcataaa
     4681 cggaaaatac gtctggatga tcaactggta atcgctcaac cataccactt actgggattt
     4741 gtttaggtag ctgtgtatct ctgattttaa aggaaagttt atcagacatg atttcccatc
     4801 ctttacgccg atagtagggg atactgtaag gatacaaata agagatccat tgcttattct
     4861 tgcgcatatt atccaatgca acgataatta gatcttgcat caatccatgg ttagcatatt
     4921 ctggataagt tccaactcca gtcacaccac ccattttata acgtgcaccg tgaatattga
     4981 cttcacaagg atagatagcg atttgagaga tcagtttatt ttcgtggaac catccgaaca
     5041 ctttcgatac ttccaaaatc ggttttttgg aacggataaa agctcgttta ttctcaaacc
     5101 cgctttcctc gatgtctgct tcagtcactt ggaacacata ggaaagcaat tcattgaatt
     5161 gatcgatatg ttcttgagtg accggtttta attcaagatt ttctctaaaa tctttttgat
     5221 ccattttttc atctccattt attagcttga taacagtata gcaaaattct acaaaaaaaa
     5281 cttctatagc tgactaatta aaaactcttt aaattactgc ttttcgttgg tataatggaa
     5341 agtgctgatt atatgaaaag aggagacgct atggatataa agaaaatgaa agaacgccaa
     5401 gagcagattc gtaatttttc gatcatcgct catatcgacc atgggaaatc tacattggcc
     5461 gatcgtattt tagaaatgac gcacactgtg acgtctcgcg aaatgcaaga tcagctgtta
     5521 gactcgatgg atttggaacg tgaacgtgga atcacgatta aattaaatgc tgtagaactc
     5581 aattacacag cgaaaaatgg agaaacatat atctttcact tgattgacac cccagggcac
     5641 gtcgacttca cctatgaagt ctctcgaagc cttgctgcct gtgagggtgc cgttttagtg
     5701 gtcgatgcag cacaaggaat cgaagcacaa acattggcca atgtttattt agcgttagac
     5761 aatgatttgg agatcctgcc agtcatcaat aaaatcgacc tacccgcagc agatcccgaa
     5821 cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg ctagtgaagc tgtattagcc
     5881 agtgccaaat cagggattgg gattgaagat attctcgagc agatcgtgga atatgttcct
     5941 gcaccaagtg gagacttaga tgcaccgcta aaagctttga tctttgactc tatttatgat
     6001 agttaccgcg gtgttgttct aaatgtccgg atcacggacg gtatggtcaa acccggagac
     6061 aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta cagaggtcgg ggtcttctcg
     6121 ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg tcggttatat cacagcaagt
     6181 atcaaaactg tccaagatac acgagtaggg gatacagtaa cgttagcaga caatcctgcc
     6241 gctgaagctt taccaggtta ccgaaaaatg aatccgatgg tttattgtgg tctttatcca
     6301 attgatactt ctcgttacaa cgatttgcgg gaagctttag aaaaactgca attgaatgat
     6361 gctgctttac aattcgaacc agaaacctct caagcattag gttttgggtt ccgctgtgga
     6421 ttcctcggac ttcttcatat ggatgtcgtc caagaacgct tggaacggga attcaatcta
     6481 gaattgatca cgactgcacc atccgttatt taccatgtta ataaaacgga tggtacgact
     6541 gctacagtag ataacccggc agattttcct gaacctgtaa cgattcagga tgtagaagaa
     6601 ccattcgtca aagcacagat catggtccca aatgattttg tcggtgcagt aatggaactt
     6661 tctcaacgaa aacggggaga attcattacg atggattatt tagatgatta ccgagtaaat
     6721 gtcgtctata atattccgct ttctgaaatt gtctttgact ttttcgacaa attgaaatca
     6781 agcacgaaag gctatgcctc attggattat gaaatgtctg gttatcaaaa aagcaagtta
     6841 gtgaagatgg acatcttgct gaatggagaa aaagtcgatg cattaagctt tatcgttcac
     6901 cgagactttg cctatgaacg tggaaaagca atcgttgaaa aactgaaaaa actaatccca
     6961 cgccaacaat ttgaggtgcc aatccaagca gctattggac aaaaaatcgt tgcccgttca
     7021 gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt atggtggaga tgtttctcgt
     7081 aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc ggatgaaaca aatcggctcc
     7141 gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa tggacgagga cgaaccgaag
     7201 aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa ccatgtttac atggatttgc
     7261 caccggtttg ccaccggtgg t
//
LOCUS       DAZAOG010000131         7185 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000131 DAZAOG010000000
VERSION     DAZAOG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.131"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..417)
                     /locus_tag="ACHDLD_002242"
     CDS             complement(<1..417)
                     /locus_tag="ACHDLD_002242"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3363925.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSV"
     gene            complement(417..1553)
                     /locus_tag="ACHDLD_002243"
     CDS             complement(417..1553)
                     /locus_tag="ACHDLD_002243"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3363926.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1585..3036)
                     /locus_tag="ACHDLD_002244"
     CDS             complement(1585..3036)
                     /locus_tag="ACHDLD_002244"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN3363927.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3080..3844)
                     /locus_tag="ACHDLD_002245"
     CDS             complement(3080..3844)
                     /locus_tag="ACHDLD_002245"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN3363928.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3872..4570)
                     /locus_tag="ACHDLD_002246"
     CDS             complement(3872..4570)
                     /locus_tag="ACHDLD_002246"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN3363929.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4582..5361)
                     /locus_tag="ACHDLD_002247"
     CDS             complement(4582..5361)
                     /locus_tag="ACHDLD_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN3363930.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5377..6321)
                     /locus_tag="ACHDLD_002248"
     CDS             complement(5377..6321)
                     /locus_tag="ACHDLD_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3363931.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6367..7146)
                     /locus_tag="ACHDLD_002249"
     CDS             complement(6367..7146)
                     /locus_tag="ACHDLD_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363932.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1966 a         1544 c         1133 g         2542 t
ORIGIN      
        1 aacagacgtt aacttacatc gataatattg ctttaaaaga tatttaaaat ctctatctat
       61 cgatgtatta tggctatgtg ctatccgtat cggaatccca gctttttttg cagcttttaa
      121 aggtaaataa ctcatcgcat caatatgaga atgaacaatc tcatattctg gatgatcctt
      181 aaaaaattgt tccatccatt taaaatattt aggataattt tgtggataaa gtctcggtgc
      241 ataatagacc tttcctccaa gacttattat ttcctcatca taatcacttt tatatggacg
      301 atgtgtcaaa aaatcaaact gaattttagt tcgatctata ttcctataat agttcattag
      361 catcgtctcc aaccctgcac gatgcatatc attcacacac tgtaatattc gaatcattat
      421 atatttcccc tatacaaatt taatgtttct tgtacgattt tatcccaatt atatttacta
      481 caaataaact cagtagcttg attcttcaaa actttaacca tttccgattg attgcatgcc
      541 tcttctaatc ttatctttaa atcacttaca tccgatttct taaaaatcat agccttgtct
      601 tctactactt ccgtacactc tgagatattt gaaactatac aacagttccc ataactcatt
      661 gcttctaaca aacttaatgg cattccttct aaatcacttg gaagtgtata gatatacgca
      721 ttactatata attcttctaa ttcctgtcct tgtacaaatc cagtaaatat aattcgctca
      781 tcaccttttg ctaattcctt tagttcattt gcaaattcat ctgtatcaga gcttcctccg
      841 gctattataa gttttttatc tgtctgaaca tctttaaatg cctcaattag ataccgaatt
      901 cctttttcgg gaaccaatcg ccccaaaaac aaaatataag aatcttttgt taatccatac
      961 ttttctgtaa tcaatcctgc tgattttatt tcaatttttt taacgccatt tggtataaac
     1021 cttgtttttc ttccatatat atcttcaaaa taattttgga cgctttcact aagtacaatt
     1081 atttcatctg caaattttac agcacacttt tcccctaaca tgatgtatgt actagcaagc
     1141 ttgttccatt tttctctttg atgatctaga ccatgaattg ttgcaacaca tcttttccca
     1201 aaaagttttg gcaaccacaa catagcacat ggtccttctg catgaaaatg aacaacatca
     1261 tattttccga atgctgctct aattccacca aatactgaag aactcattgc tgcaattcct
     1321 tttatattta atgtaaaaat agtctttaat tttacacctt tatattcttt atttttatgc
     1381 tggtcaaatt cctttccgct aacatgatga ccacttcgat tatagcaagt cacttcatgc
     1441 ccaagtgcaa ccattcgcgt tgccaattct tctaccacta tctcaattcc accttctctt
     1501 gatggaattc ttttatgacc tagcatacag atttttaatt tttttcttgc catcacgttt
     1561 tactccttct atagtatctt tatcttacat ggaaccgttt ctatgaagca cagttttgac
     1621 tgtcctaaac aaaattctaa aatctaatcc catactccaa ttcctgatat attctgcatc
     1681 aagttttaca acctcttcga agtctgtgat atcacttcta ccactcacct gccacattcc
     1741 agtaatccca ggtttcatcg caattctcgc acgatgatga aaatcatact tctcatattc
     1801 tgacttcaag caaggtctaa accccaccag gctcattttt cccattagga cattataaaa
     1861 ttgtggaaac tcatccaatg atgtagatcg aataaattgt ccaattcctg tcttctttgt
     1921 tccatcagga agtattttat tgccaatgat tcttgggtca aaatccatct taaacatgag
     1981 attattactc atcttatttt ctttcatcaa ctctgctttg cgttcttctg catccatata
     2041 catactacgg aacttataca tcttaaatgt cttaccattt tgaccaatac gttcttgtgc
     2101 aaagaaaatc ggccctggtg atttgatgta aataactggt gctacgaaga taaaaataac
     2161 acctgttgca atacatccaa taattccagc aagaatgtca atcgttctct ttataaatgc
     2221 ttgcctcatc gtcataacat taatactaga tgtaataaca gtataatcac caactcggcc
     2281 aataaattgt tttccacgtg tactatcaga cacttttgta agattcatat gtacagcaag
     2341 tcccatttcc gaacatctgg cgattaattc tttgggatat ggaacacttt cgtcaacatt
     2401 gatgaatact tcatctaccc aattctgtaa aagatattcc gctgcattat cagcatttgc
     2461 aactactggg acacctccga tggtagttcc atacatatct ctgtctacta caatcacacc
     2521 actaagttta tacatgccat aattcttctc tttaagatta cgaactacat tttttgcaat
     2581 cttttgagat gttacaataa ctaacgatac atcttcaccg tttttcatgc ggtgatgcaa
     2641 caaccctttc caaagtatac gaattatata tgttaataat ccatacacca cccccattat
     2701 aaataaagaa actctggaat aatcatttcc atcctgcatt gtaataaggt ataaactgca
     2761 cacaagtaac agcataaaag attgctttat agaagctaca gcctctttat aatatcctct
     2821 ttttaaaaca tttttatatg cctcaaataa aaatataatc gtaagattct caagttctat
     2881 aaaaattgcc atgtggcgat aaataggaat cacatacgga ttccagtcac cctgtcgcat
     2941 tacatatgat atataaaaag caagctggac acaaaataag tctaataaaa taaaatcaat
     3001 atgtttagac caaccacttg agttttttct atacatctct cactctctcc tcatttcctt
     3061 tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc tcttcataga
     3121 ttggataatt gatctcatcc ccgttgatca gatcctttgc gacttgtttc atctgtacta
     3181 ctttttcctt accaaactcc ttggcaatct gctcatagca ctcttttaag taaaaggtgc
     3241 gcttagagac cccgtgtgca tcagaagcag ccatctgcac gagattatgt ttgaccattg
     3301 ttttggctgt tttctggata tctttgccaa atacaccaac ataacttggc gcagtaagtt
     3361 gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttct cgaaaatgcg
     3421 cgttgcgttc tggatggacg atcactggta tctttcctag ttctaacaac tcaaaaaaaa
     3481 gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga tatgtatcat
     3541 ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc gatgagcgtt ccggtaatac
     3601 gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat tcggcttgta
     3661 acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca ttattgtggt
     3721 gtggtgtgca taaaatatgc gtaatgcctt gctgaatcgc tttttcagcc attgcaatac
     3781 ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa tgtagatcga
     3841 tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa ccagcatctt
     3901 tgctgtgttc tgctccgttg tatacgacac ctaagactcg tgcttgtacc atatctaaca
     3961 gttcttttgc tttagtcaat gattcttttc gtgacgtatt ttcccgtacg accaataagg
     4021 tgccatccgt ttttgaagac atgatctgtg catctgttac ggcaacgact ggcggcatgt
     4081 cgaagatcac tacatcaaat agttgtcttg cttctgctaa aatctgatcc attcttggcg
     4141 aacttagtag ttctgatgga tttggtggtt taggtccact tggcagaatc gacaaattgt
     4201 cgactggtgt tcgttggatc tcatctgcta cacttcctga tgaactaaga acagtactta
     4261 atccgcttgc gttgttcaac ttgaacgttt tatagatgac gggcttacgt aagtctgcat
     4321 cgactagcag cacccgttga ccagatttgg caaaaacaac ggcaatgttt gctgcagtgg
     4381 tcgatttccc ttctccaggt cctgatgaag tcacaacaat cgttttgatt tgctgaccag
     4441 ctgcggaggc aaactggata ttcgtccgaa tcgtacgata ttgttcagcg attggtgaag
     4501 agggattggt gagtgtgacc aaactgactg catgagtttg ggctgctttt tgtttctgtg
     4561 ttcgtgccat tttctttcct cctagactct tgagcggctt ctgcgtgatg gcgctgtttt
     4621 atcgtttgta gcttttggtt gtgtctgagc tggtcctttt ttacggatac cagcattcaa
     4681 ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg tatctgtcac
     4741 atatttttca tctttcactg tccgatcgag taattccaat aagaatgcca gacctacacc
     4801 taccatcatt cctaacatta agccagtcat taaattcaat ttattatttg gcgaaacagg
     4861 tgtcgtattg gcaactgcac ctgaaatgat cgagatttta tcaacattca tcacatcttt
     4921 tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag ccactgcatt
     4981 cgtacttatt gcctgaatcg agaacatttg tgaattttct gcctgattaa ctgagatggc
     5041 atctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac gatctttgac
     5101 ttcattgagc actaaatcac tgacgatcat atctttatac gtattgatca tttgcaggtt
     5161 cgtattgaca tcattgacat ttgcagtgtc tgattgtggc agtgtcacga tcaattgtgc
     5221 ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga tggccaagcc
     5281 agcaaacatt gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa aaatttcttt
     5341 caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta gattcatttg
     5401 atcttttaat tcttttttca cacgatcaag ttcttgatca ctgacttttt gataggagat
     5461 attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac caaatgcctc
     5521 ccgataatct aaagcaattt ttttcatttc atcaaagttc atgtctgttt tcatattcga
     5581 ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca atactttttt
     5641 cgctacgcct tctacgatct ttctttggcg cccttgtcta ccgtaatcac catttgggtc
     5701 ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtaa ttttaccgat
     5761 tggaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga taccaccaac
     5821 tgcatcaacg agttccttta gccctttcat attgatagag acatagtgat cgattgggat
     5881 gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg cgtaagcgtg
     5941 gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatcac gaggaatgct
     6001 gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg tatctgatct
     6061 tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa cagagaaaga
     6121 ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt ccactgattc
     6181 gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta ctcctacaat
     6241 tgctaatact aatacgataa gaactgctaa aataatcaga aatatttttt tccctcttga
     6301 ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga atcaacgttt
     6361 taactgtcaa tagcttatat tgctatagcc catttgctca gcaacagttt gtgcttttgt
     6421 tgcgtatgct gcgcttattt tttcattccg gatctgctct atttgactga gcaccgtcaa
     6481 atattgatcg taagtagcgg tggcagtcac ttgaccatcc tttaacatcg tatcgaataa
     6541 ctgatctagt tgcgtgactt gcgcgatctg aatatcagcg aatcctagat attgcataac
     6601 aaccgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac gagcgacatc
     6661 tgcacttgtc accttttctt tgatgatgac atcgtctaca gcttgctgcc agtttgatac
     6721 atttttagta aataaatcgt tgacagctgt ctggatcttc agttttgatt ctgcactatc
     6781 taactgtgtc acgattgctt tcttctcatc ttggattgca acagcttctt taggcaattc
     6841 tgattctttg atgtcgtaat catctgctga aagtttgaca gaggatagct tatttgacaa
     6901 acttgtgatt gtttcttctg ttatttcagg gtttagaaaa attttttctt ttgtactata
     6961 cagcttgtct acttcttttg ataaagaagt caaggtgtct ttttgattgg tgatggcaga
     7021 ttctgcataa ttgatccgtt gctgatgttg ccgctgatta ttgatatggt atccaaacaa
     7081 acagatcgca atagcacctg cacctaccgc cacagcagtc aatgacttga cgaatggctt
     7141 ttccatagat tcactcctaa gtattgatga tagataaatt aagat
//
LOCUS       DAZAOG010000132         7130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000132 DAZAOG010000000
VERSION     DAZAOG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.132"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1172)
                     /locus_tag="ACHDLD_002250"
     CDS             complement(42..1172)
                     /locus_tag="ACHDLD_002250"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFN3363933.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1231..1458)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1820..3022)
                     /locus_tag="ACHDLD_002251"
     CDS             complement(1820..3022)
                     /locus_tag="ACHDLD_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363934.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3082..3285)
                     /locus_tag="ACHDLD_002252"
     CDS             complement(3082..3285)
                     /locus_tag="ACHDLD_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3363935.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3427..4491)
                     /locus_tag="ACHDLD_002253"
     CDS             complement(3427..4491)
                     /locus_tag="ACHDLD_002253"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3363936.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4552..4659)
                     /locus_tag="ACHDLD_002254"
     CDS             complement(4552..4659)
                     /locus_tag="ACHDLD_002254"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363937.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4822..5634
                     /locus_tag="ACHDLD_002255"
     CDS             4822..5634
                     /locus_tag="ACHDLD_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3363938.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            5789..7128
                     /locus_tag="ACHDLD_002256"
     CDS             join(5789..6230,6230..7128)
                     /locus_tag="ACHDLD_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN3363939.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         2271 a         1365 c         1157 g         2337 t
ORIGIN      
        1 ccgacttttt gggacgcttt tttttgccaa gcctcacatt tttatagcca aacttctttt
       61 gcgacttgct gcaataattg gattttcttc cattgttctt cttctgttaa atggtttcct
      121 tcttctgttg aagcaaaccc acattgagga ctgatactta gattttccaa tggtacaaat
      181 tgttgtgctt cttttattcg ttcttttatt ttctccgtat cttccaaatc tggaaactta
      241 gaagtaacta atcctaaaac aactttactt tctttgttac tccaatgtct taaaggagta
      301 aaatctcctg ctcggtcaga atcgtattcc aaaaagaaac catcataatt tgttgaaaac
      361 aactcttctg caatgggttc atatccaccg gaaaacagcc aagaagaagc gtgattcccg
      421 cggcagatat gtgttgtgat cgtcaaatct tctggtttgt cttccagtgc gttattgata
      481 atctctgtac aaatatgggc catttctttc ggatcaattc ctttttgaag aagtgcttct
      541 cttttagttt gatcagctag aaacgcccaa tttgtatcat cgaattgcag gtaacggcaa
      601 cctttttcat aaaaagcatg aatcgctgct ttgtaagtag aggacaagtc ctcataaaaa
      661 tcagatagat caggataaat ctccttgatt tttgaagaac catcattaga taggatctct
      721 tgtcgtaaaa tcatcgtggg actagggatc gttgcttttg caacaaaagt atcatctgtt
      781 tccactactt ctttcagaaa agcaaaatct tttaaaaatg ggtgatttgg attgaaagat
      841 aatttatcta ctacccgaat attataagct ggtgctgctt ttccgtggaa tgtttgatta
      901 tatccagttt ctggaacata gccttcgata ccatttagtt cttctaaaaa atcaagatgc
      961 caaaatccac gccgaaattc accatctgta attcctttga tgccgactgc tttttggtct
     1021 tccactaatt tttttatttc ctggtcttcg attgcacgaa gttcttcttt tgaaagtaag
     1081 ccttcttgaa ctttttttct tgcttctttg atcgattgcg ggcgtaagaa acttccgaca
     1141 tgatctactg taaatggtct atttttgctc atttcactca ctcttttctt tgttttttgt
     1201 aaaaaaaatc cttttactag caaatactag taaaaggacg attatactcg tggtaccacc
     1261 ttttttcaga aaaaacattc tttctctcag cctgacaata aaatcagtgg tcttggtaac
     1321 gaaagactgt tcgtttttgc ctacctttcg acaaaaaatc ctctaaggcc attttcgaca
     1381 gtcaacttct agctttttac accaaccaaa gcctctcttg aaccattttc tgtctacttt
     1441 ccttctcatc ggatactatt agaataaaat tagaatagca tgtttgaaag aaaagtcaat
     1501 agggaataag aacaaacaaa aactaatttg ttattcttag taatttgttt gtggaataca
     1561 ataaagcttt tctaatatga taattgttca gtcgtccttt catcttagat cacttcctta
     1621 aaatgatcat acccgtttca accacataaa aaaggactta attttgaaaa atttcccatc
     1681 aaaactaagt cctttttatt atagttatgc aaatttgctt attttctcgc tatttccgat
     1741 acatcttgaa caggagcaat tcgactgaaa acactggtgc aaaagcattt atttgtctta
     1801 tggtagaacc gtggaaaact cagactggta ataaattgtc attttttgaa agaatagcgc
     1861 cctcttgaac attatcaatc gcacttacca cttgttgatc tgactttgca tataagtgac
     1921 cgtaagttcc taaagtgatt tgaatattcg catggcccaa tcgttcttga atagatagcg
     1981 gttgaatatt ctgctcaatt agaaatgcaa catgagaatg tcgtagcgcg tgaagcttaa
     2041 tcggtttgac acccgctttt ttagcagctt cttttaacca gttcgcaaaa gaataaggtg
     2101 gcgtaaatgt tccgtccaac tgagcaacat attcaaaatt tccaatacgt ttttgcaagt
     2161 cataccactg tctgagtttt aaggaaagct tcttccccaa cccaatcgtt cgataagcac
     2221 atttagtttt gggagtcgaa aagtaccaat catcttttgt attataaatc aatgtcttct
     2281 caatcgttat cgtgtttttc tcaaaatcta cattcgacca agacaaagct tcaagttctc
     2341 caatccgaat gccagtataa aagagcattt catacacaat cttccgatgg tgggtttgaa
     2401 tcttatcatc cttcaattca ttcatcactt tttgaaactc actgtgttcc caaaactcaa
     2461 ctttcttatt ctcagaagga agcgaagaaa catcttcaac gggattttta gcaatcaccc
     2521 cctctttaac agcccgatcc aatacaattt ttaggtgccc tcgaatccga ttgatataag
     2581 attttgataa cgtttcgcca ttaacttttt tccgtgaatt acgtgactct gtctggctta
     2641 gttcaaataa ccaatcctca atatcgtgac ttgtaatatc tcgaacacga aaatgataga
     2701 aaaactgcat acgcttttcg aaaatgaatt gcgcattctc atatgtttga cttttgacct
     2761 gtctctcgta ccattttaca aacctatcct tgtaaaactc ttcgaaagtc atttgattgg
     2821 tagaacacag ttgattagga attgtcttgt taaaatcatg tgtttccatg tacttgtcat
     2881 aagcaatttt ggcatctttt tgtgaagcat aacctcgttt agtcttctga acctttcttc
     2941 cattttcatc tatacctaga tacgctcgga agaaccattt ttttgtcttt ttatcttggt
     3001 aaatattttt aatttttggc atcgttccat tcctccctgt acttgagaaa agctacttta
     3061 atagtaccgc gcttgtactg tttattccgc aacttgaatg tgaaaaagat gttcaattac
     3121 tcgggatggc accacaccaa cttggcgatt attataaaaa tcaatacctt caatccttgc
     3181 aagatatgcc ttgctctctt taatcattcg tgcagcttga ctaggtttaa agcccatctc
     3241 tatcaaaatt gcacgattta ccatgaaatc acattttgtt tccattctct cacctcctga
     3301 ttctttactc cttttaaaat aatcctcttt ataatctatt gtttctcatt cgatggttgt
     3361 gttatactcg tcttgtcgag gcggagtacg ttgcacaact tgttgtgtaa gcagtacttt
     3421 gcttttttag tagcttgttt tctcatttga tatactttgt aattttcgtt cataatactc
     3481 aacctcctcc tgataatact ccgggtctgc cttcatattt tggagaagtt tgatctgtct
     3541 tggattcatt tctccttgtt caataatcaa aggcaacaag gaagaatgaa acgtttgatc
     3601 gacctgtgca ataaaaacta atgatggtga aacaccatga attaaccaac tcatagtttt
     3661 ctcaaaactt tgaggaattg tatctaaaga aatggtgatt gcgccataat tgctaataaa
     3721 ggctgcccat ttggcatcaa cttttgcacc tagatcattt ggatcacggt cataaaaaca
     3781 aattgctcca ttaagtaact caaagaacag tttggttaaa tcatgggttt ttgaaataat
     3841 ctttgccaag ctttgcgctt tctcttttcg ataccgtagt tcaaagcgat tctttacttt
     3901 tgcctcttct aaaggaattc ctcgtcgctt ccgttgttca tggtccttct gataaaagca
     3961 aaaacgacac tgactcttct ttgagcctaa atacaaggtc tcaccatttg attcattgtt
     4021 tatgatatcc gtaccgccat gagattcact cacttgaaaa gtggtccaca catggcctag
     4081 tattacctta tgcttaagtt ctggaatgct gagactcccc acaaaatcat ctaaagtaaa
     4141 atcaatccgt gtaaagttcc cctgataatc taatacctta cgaaaaaact gttgccaatt
     4201 tgtttttcgt gtttttagcc atcttgctaa gtgtttacag ccttgacctg aaaactccat
     4261 cacagttcct ttaaccgcat cgtcaatgga ataccgaatc gtgattactt gattccacac
     4321 gtattggcca atatagccct ttggcgcata atccagttcc tcaaaacgtt caatgggcat
     4381 ttcaataatc tcttcaatta gctttaaatg ttgattcgag aaaaagtgaa tggttagata
     4441 atcaatcaaa acacggttaa ctggaacgac ctcttcttgc aaactatcca acacaagatt
     4501 caatttttct tcacttaatt gtctcgttcc tttttctact ttcgataata actatctcga
     4561 aatccccact ttttctgcta aatatcgttg tgtccacccc ctttcttctc gagaaactcg
     4621 aatggcttca ccgagatcat gttgattaac ttggttcatc gacaaacctc cttctgctat
     4681 ttacttgtca aagaacctat tgaacttatt ttaagtacat ttttattgta cgcctgtatt
     4741 tattttaagt caatatgttt tgactttttt taagtacaca cgtatactaa atttaagaac
     4801 ttatcctgag gaggagtttt catggagata gacaagcaag cagtcggcaa tcgaattcgg
     4861 cagattcgcc aagaattaaa gttgagtatg gagaaatttg gcaaactaat tggtgattta
     4921 ccgcgaagta cagtcaataa ctgggaacgt gggatcaacc ttccaaaaac cgaaaccctt
     4981 caccaaatcg cagagatagg tcatgtcacc aatgaatatc ttttgtatgg tgatcaagag
     5041 aatcaataca ttttagaaat gctccaaaaa aaggcaggta agcttgatcc taagattgaa
     5101 ggtctcatag tagacgaaat caaacacgct gaccttgtta gtgaaaaaga aatggatcga
     5161 atgattgaat tttttattac aaatctcatt ccaccaactg aacaagacca gtttactttc
     5221 caatgtatcg atgaaaaaaa gcagctttat cttggttcaa ctaattttgg taaggaagcc
     5281 cagctctatt tgcaacatga taatcaaaat catattctgc acatgatgcc ctttaccttc
     5341 tccaacttta gtgtggatcg catactcgtc tatttagcaa atcaaaaatc ctattcctat
     5401 tttgggaaac atttacccaa ggaactaggt gagaaagcca tccttctata ctcaattaat
     5461 caattgacca gcgatgtgcg aattgccccg ttggtctatt cagaagaaac cgcttcttat
     5521 cagtacacag aggacaatca atatctacta gaacagcaat atctttatct accattcgtg
     5581 atggaagtcg agaaagaacg cctgttaaat gcagcgtatc catcatcaaa atgaggtgac
     5641 aaaattgata agcacgtgac aaagaaaata ctgctaggac actccccaat gggtagttgt
     5701 cacagtttcc tgttctttgt tagccccctg ttagatgatg tgcaccccaa aagttagact
     5761 agaaatctaa ttaaaagggg tgcttttttt gcggaaatat acatttgttt ttaagaaaaa
     5821 agtagtttca gactatttaa ataacgaagg cggctacaaa tatcttgcac ataaatatca
     5881 aataaatcgt acactggtta gacattgggt aaggatttat aactatcatg gttgggaagg
     5941 cttggttgga ggtggcaaaa gctacactac aaaatttaaa cttgatgtta tagaatatat
     6001 ggaaacaaat ggtctttcta tccaagaaac tgctaaaaaa tttaatatcg gttcaaatag
     6061 aactctaagt aaatggatag agcaatatga agaaggcggt gcttcttcac ttgagagcca
     6121 aaaaaggggc agaaaaatta gtatgaattc caagctaaac attcctaaaa aacttaaaga
     6181 tgagtctctt gaagaagaag ttattcgttt aagagcagag aacgcatatt taaaaaagtt
     6241 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
     6301 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
     6361 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
     6421 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
     6481 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
     6541 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
     6601 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
     6661 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
     6721 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
     6781 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
     6841 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
     6901 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
     6961 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
     7021 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
     7081 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZAOG010000133         7125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000133 DAZAOG010000000
VERSION     DAZAOG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.133"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..331)
                     /locus_tag="ACHDLD_002257"
     CDS             complement(71..331)
                     /locus_tag="ACHDLD_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363940.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(468..881)
                     /locus_tag="ACHDLD_002258"
     CDS             complement(468..881)
                     /locus_tag="ACHDLD_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN3363941.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1337..1822)
                     /locus_tag="ACHDLD_002259"
     CDS             complement(1337..1822)
                     /locus_tag="ACHDLD_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363942.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1961..2176)
                     /locus_tag="ACHDLD_002260"
     CDS             complement(1961..2176)
                     /locus_tag="ACHDLD_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN3363943.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2400..3200
                     /locus_tag="ACHDLD_002261"
     CDS             2400..3200
                     /locus_tag="ACHDLD_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3363944.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3219..5087
                     /locus_tag="ACHDLD_002262"
     CDS             3219..5087
                     /locus_tag="ACHDLD_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN3363945.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5080..5571
                     /locus_tag="ACHDLD_002263"
     CDS             5080..5571
                     /locus_tag="ACHDLD_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN3363946.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5559..6113
                     /locus_tag="ACHDLD_002264"
     CDS             5559..6113
                     /locus_tag="ACHDLD_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN3363947.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6131..>7125
                     /locus_tag="ACHDLD_002265"
     CDS             6131..>7125
                     /locus_tag="ACHDLD_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN3363948.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2508 a         1139 c         1375 g         2103 t
ORIGIN      
        1 aacttgtatg aataacagct gaaaagatct gcaccaaata aaaaacttcc agccaaaact
       61 gactggaagt ttattttttt ctgaaatatt ctaacacaaa tatgaaaaag atggccgaag
      121 ctaataagcc gatttcgtag caaaacaatg agataccaga gtcgtaataa taaaatcctc
      181 taatcttatg gataaaatag atcatcggac aagcagataa gagtaagtat ttgttaggcc
      241 ttttgaataa gtaaaagaaa gcactggtca ttatgataaa tggtatccaa aacaaaagtt
      301 tacttaagac aatcaaatga tcaactccca tattttttac caagagagat cataacatct
      361 ttatcgtaaa aacaaaatat ttttttgctc attttgaaaa tttaaacata ctcgttctta
      421 tccaaatata taaaaatcgc ccctcattca tcgagaggcg ataagttcta atttaactca
      481 attttttttg tttcttttgg ctgataagct gttttaggta ataccaattt cagcacaccg
      541 tttttaaagg ttccagtgat tttttcttca tcaacattag gaagatagaa tgaacgtttg
      601 taagagaccg aatgtctttc tttccgtaca tagttgccat cttctttatc ttctgtatgg
      661 gactcatgat tggcggagat cgtcaaagta ttatctgaat attcaactgt cgtgtcttct
      721 ttgtcacagc caggcatatc agcagttagt ttgtattctt tttcattttc gactaaatct
      781 acttttggat aacttgaaac acctaaaaaa tcattgaatg ctggactaaa cacgtcattg
      841 aaatcaggga acatgtcgcg aatgctaggt acatttgcca tttttgatca tcctttcaat
      901 aagtattttt tatttatccc tatttacgta ataactctat tttgaaaaaa atgcaagtat
      961 aatggttagc tttgtgaata tttcatctaa tacgatacaa aggctatctt tctaatttga
     1021 aaaagggatc attaaaataa taagaagaaa atatgtcaaa aggtaaaaaa gtaaattctt
     1081 ctatccaaat atagaaaaat tgaatctaga aaaccgcccg acaataaatt tcattaactc
     1141 agttgaccga aaatgaagta gataccaaaa atttatcaag agagcaagta gagaagtgag
     1201 tatattcata agagaaatag ctgtaacgaa ttattaggtt aatgcaaatg cttatttata
     1261 ctccgtataa ctttctcaga aaaaatgata ccaataatga cttgctagta ctgtaaatcc
     1321 gtataatatc atctaatcaa aatggtgttc catttgcatc tgtcatcgta atcgatcctt
     1381 cgggtgacac tcgataaaaa ccaacgcttc cagatcctcc ttgggaacgg atttgtttgc
     1441 tggaagctgt gaacaaataa tctcgatcaa ttttttgata aaagctaata acaatatcgt
     1501 cactttgtaa ttcggaacgg tgggcaataa gaaatagaag tgctttgtca gagttttcta
     1561 atggaagaga aggtacagtg ctttcttgca tttcagatgt tgaagaaatg ctaaacgact
     1621 gaatacttga ttggctatgt tgcgaatcta cattaccagt agaagtatta gtaatggaag
     1681 tagtagaagt acttttagtt gaaccaacct cagttgttga aatggctgct gaagattctt
     1741 taacagaatt ctttgtttta tatccacagg cagaaaaaag tattacagtt cccaacaagc
     1801 ctgcgtaaat ccctttatgc atgatagctc ctcctaaaat agattatctt tattcagtat
     1861 atcacttaaa caaagaaaag cagatggttt aaaatgaaaa aaccttccca ataaagggaa
     1921 ggttcatgaa gataataact tttttaataa ttatagcttt ctaatatctt tcatcaactt
     1981 ttctagtaat cattgggaaa aatttcaagc agtagatcgc acaaatacct acgacgatat
     2041 aaatgatctt cgcaaaaata gttgttgaac caccagcaat catagctact aaatcaagtt
     2101 cgaacaagcc taccaacagc cagttcaacc ctcccacaat caataaagtt aatgcaatag
     2161 catctaaagt tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa
     2221 agagaaacat tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa
     2281 ttttttttgc tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta
     2341 atttttttta gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata
     2401 tgtcggattg gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa
     2461 ttggaagaag tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg
     2521 taactgaatg ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta
     2581 aaacggacat aaaagaaggg atatataaag taatgaaaca ttttaagacg atcgatggtc
     2641 tagtcaataa tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa
     2701 gcaagtatga attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg
     2761 tatatttgat gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg
     2821 tgaatctttc ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta
     2881 caaaagcagc gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg
     2941 ttcgtgtggt aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat
     3001 atgaagaagc tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca
     3061 gtcgaacaag tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag
     3121 tctgctatta tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag
     3181 gaggtaaaac aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt
     3241 accaattatt gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag
     3301 aaatcaaaac cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg
     3361 ccggtattgc aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg
     3421 atttttttga tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat
     3481 ccaaaagaat cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg
     3541 atgatttgtc tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat
     3601 taaaagagat gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa
     3661 gaataatagg agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact
     3721 tttcagagac gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga
     3781 agaagacgaa tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt
     3841 tgaatagaat ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt
     3901 ttgaatatat ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa
     3961 ctttgagcca atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta
     4021 taacgatgat aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc
     4081 gtattcgacg attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac
     4141 agcacctctc tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt
     4201 atggtgagat agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag
     4261 cattagaagc tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt
     4321 atttggagtt agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg
     4381 ttcatacgac aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg
     4441 ggagggatat acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca
     4501 aatatttcgc tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga
     4561 tccatttgac taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa
     4621 aaaagcgatt agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa
     4681 agacgtatca agaatattta aacaaattag tcgctccttt gatcagaggc tattatgtgg
     4741 atgaagaatt tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca
     4801 aagaatttgc ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag
     4861 ggctaataac tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg
     4921 ccatacctga agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc
     4981 tatttgaact cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat
     5041 catttttaaa actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc
     5101 atagttttcg cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag
     5161 catttcaatc aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga
     5221 cctgggaaaa tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt
     5281 ttagaagtca gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc
     5341 tatgcgggtg aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat
     5401 cgtttgacac aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga
     5461 aatgaaaaaa gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata
     5521 tggaaaagta cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg
     5581 ctgagggatt tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag
     5641 gaatcgtacc agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag
     5701 gtgaagaccg agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc
     5761 ttgtgttacc gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc
     5821 gtttccttcc agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata
     5881 cgagcaatgg tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg
     5941 ctcaagggat cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg
     6001 tgggactgct gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat
     6061 gcagacagca aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg
     6121 agagttttaa atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc
     6181 tttgattatt aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga
     6241 gaggccaatg atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt
     6301 caaaacatca atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct
     6361 tagatgtgga atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa
     6421 aagtggccca ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga
     6481 acaaatacag ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc
     6541 agataaaaag tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca
     6601 aaactggata gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc
     6661 agcaagagaa gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc
     6721 ccttttgatt ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc
     6781 acctttagca ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt
     6841 tttatctcct ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg
     6901 tgtagagatt gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat
     6961 caatactcaa aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc
     7021 tgagacagta gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc
     7081 acgagttatc attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAZAOG010000134         6966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000134 DAZAOG010000000
VERSION     DAZAOG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.134"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACHDLD_002266"
     CDS             14..1168
                     /locus_tag="ACHDLD_002266"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN3363949.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1190..1942)
                     /locus_tag="ACHDLD_002267"
     CDS             complement(1190..1942)
                     /locus_tag="ACHDLD_002267"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3363950.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2306..4972)
                     /locus_tag="ACHDLD_002268"
     CDS             complement(2306..4972)
                     /locus_tag="ACHDLD_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3363951.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5313..6764
                     /gene="cls"
                     /locus_tag="ACHDLD_002269"
     CDS             5313..6764
                     /gene="cls"
                     /locus_tag="ACHDLD_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN3363952.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2049 a         1446 c         1272 g         2199 t
ORIGIN      
        1 aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc ctaatttata
       61 tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat accatatagg
      121 gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta cactaggagc
      181 aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca tggtcatcgt
      241 cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc accatttact
      301 gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg gtggcaccgc
      361 agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag tactagtcat
      421 agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc aaggtgtcgg
      481 tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag acgacagcgt
      541 atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat ttcctgttgt
      601 tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt ggaatcgaca
      661 taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc atattccgta
      721 tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg aagaaaatca
      781 ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa tcggtaatct
      841 ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga aatcactaca
      901 gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg aattttttac
      961 aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc aagagatgct
     1021 agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg agacgcttcc
     1081 tgaacacgga gaatgtgcag aatatacttc agatgtacca ttttcgatta caaagatcga
     1141 aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt cacgagtcgt
     1201 gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc ttagtcgtac
     1261 taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca acaggatctt
     1321 ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc tgtgtaaaat
     1381 aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt tcaaattgat
     1441 ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc ttttcgatag
     1501 gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat ttgcttgact
     1561 ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct gctgtaaaaa
     1621 ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg ataaaggaaa
     1681 catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt cctgaagaaa
     1741 aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg ccagatcctc
     1801 cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga taatttttta
     1861 catcttcagc aatagttact tgcgattctt gctggatttc ataatattct tcagcaaatt
     1921 catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg ttttcacttt
     1981 tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat aataaaatga
     2041 ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt gtcaaagtcc
     2101 ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca aaatgctgat
     2161 tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca agtcacaata
     2221 ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga attaaacggc
     2281 aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca atgatcaatt
     2341 tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta aagacttctg
     2401 tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc attgttaagc
     2461 agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag aagaaccctg
     2521 cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc cgacaccaaa
     2581 tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg ccaagccatt
     2641 gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa atcaatgttt
     2701 tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta gcaggttcag
     2761 ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg atgaacaaca
     2821 gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg atcgcaatga
     2881 tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta tcaaacacat
     2941 tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc aataccatcg
     3001 ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg tctgcttgtt
     3061 tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga cctttatttt
     3121 gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa acagtgactt
     3181 tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc ccggtcaaag
     3241 ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct gttgttttgt
     3301 ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt accgcctgag
     3361 tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc aaatcgtctt
     3421 catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct agtacacgta
     3481 atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat tcatccgtca
     3541 tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg atatctggtc
     3601 cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac attaattttc
     3661 gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt tcctgataag
     3721 gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg cccaatttag
     3781 taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc aagcgacgtt
     3841 cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca ttacctaaaa
     3901 agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct gtacagatga
     3961 tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca tgacgttttg
     4021 ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat gcttctggaa
     4081 tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct gcagtcatat
     4141 ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc aataaagaca
     4201 atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt tgtaacggag
     4261 tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc gtattattcc
     4321 cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca ctgaatacca
     4381 tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct ttttctgcag
     4441 aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca tctagtaaac
     4501 gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct accacaagtt
     4561 cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg ggcgctgaaa
     4621 ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca ctgattatag
     4681 aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc atgaccatct
     4741 gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt tctaagaaca
     4801 atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct ctttctaatt
     4861 tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc tgcatcaacg
     4921 agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc aaatttcatc
     4981 ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta agacaaatac
     5041 gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg ttaacttact
     5101 gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt caactgtact
     5161 aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta cctacatttt
     5221 catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat catgctatac
     5281 taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata accgcccttt
     5341 atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga gatgtagcag
     5401 ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt gtattatatt
     5461 tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa agtgacttga
     5521 aagaattaga aaactttcag tcttttagag gagataccat tgaacattac gatcctgaca
     5581 tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat cgtatgccgc
     5641 tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg gattcactgc
     5701 ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca tttgtgacag
     5761 ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa ggcgtggaag
     5821 ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat ttcaatcgtc
     5881 taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca ttacttcgtt
     5941 tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag attagttata
     6001 ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc ggctattggc
     6061 gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt cgcttcttaa
     6121 cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg aattatttct
     6181 ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag cttgtttcaa
     6241 gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg attacttctg
     6301 ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt gtcattgctg
     6361 ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc aacaaaccgg
     6421 atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc aaggaaaatg
     6481 tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata atggatgatg
     6541 aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg aattttgaaa
     6601 caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag ttcaaagaag
     6661 atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct aactggctat
     6721 tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt tattcaagta
     6781 gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag tcccttcttc
     6841 cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     6901 gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag gctcacgacc
     6961 tctttc
//
LOCUS       DAZAOG010000135         6936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000135 DAZAOG010000000
VERSION     DAZAOG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.135"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..548)
                     /locus_tag="ACHDLD_002270"
     CDS             complement(<1..548)
                     /locus_tag="ACHDLD_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3363953.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLS"
     gene            complement(572..721)
                     /gene="rpmG"
                     /locus_tag="ACHDLD_002271"
     CDS             complement(572..721)
                     /gene="rpmG"
                     /locus_tag="ACHDLD_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3363954.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(742..1119)
                     /locus_tag="ACHDLD_002272"
     CDS             complement(742..1119)
                     /locus_tag="ACHDLD_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363955.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(1151..1330)
                     /gene="rpmF"
                     /locus_tag="ACHDLD_002273"
     CDS             complement(1151..1330)
                     /gene="rpmF"
                     /locus_tag="ACHDLD_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN3363956.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(1345..1614)
                     /gene="rpsN"
                     /locus_tag="ACHDLD_002274"
     CDS             complement(1345..1614)
                     /gene="rpsN"
                     /locus_tag="ACHDLD_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN3363957.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(2137..3903)
                     /locus_tag="ACHDLD_002275"
     CDS             complement(2137..3903)
                     /locus_tag="ACHDLD_002275"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363958.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(3863..5616)
                     /locus_tag="ACHDLD_002276"
                     /pseudo
     CDS             complement(3863..5616)
                     /locus_tag="ACHDLD_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(5726..6295)
                     /locus_tag="ACHDLD_002277"
     CDS             complement(5726..6295)
                     /locus_tag="ACHDLD_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN3363959.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(6313..>6936)
                     /locus_tag="ACHDLD_002278"
     CDS             complement(6313..>6936)
                     /locus_tag="ACHDLD_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3363960.1"
                     /translation="YENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKG
                     KIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKD
                     QLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVV
                     IATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         2225 a         1326 c          959 g         2426 t
ORIGIN      
        1 tcggataaag ggttaatact tttgatttct ctctgtaatt tttttagtgc agataaattc
       61 aagttttgtt ttctagacac aaaaatgcga tctgccatga caagttgctt aagggcttgt
      121 ggttctcttt caataagttc aaaactgtca ctatcaataa cagtcaataa actatcgata
      181 tatactcttc ctcttagttg aggcgctgtt aaaagtgttt gaatgattgg ttggggatct
      241 gctatacctg tagtttcaat gatgatttgt gaaattggtt gttgatgttc ttctattgtt
      301 tccagtaaag aaatcaatat tgctaataaa tcactgcgaa gactgcaaca catacatcca
      361 ttatttagtt gtaacacccg ttcctcgcta tgaactaata atttgtgatc aatcccaact
      421 tcaccaaact cattttcaat tataataatt tcttccctgg ggtagggtga gacttgcaag
      481 gcttgattga ttagcgttgt ttttccagcc cctaaaaacc cagaaacgat tgtaactggt
      541 atgcccattt tgtcaccttt ttaccactta gctatttcac ttctttaaaa attgcccgtc
      601 tgcgcaattt tggcgaatac ttttttaatt ctaaccgttc agggttattc cgtttatttt
      661 tgctggtgag atacagacgt tctccagttt cgacacattc caaaataata ttttgacgca
      721 taaatcttcc tccttcaata cttatcgatc ttcaagatat gcttgaattt gttgtgcatc
      781 ttcaaaagtt aactcgccag attccaattg tgaaactaaa ttttctttca tatcaacagg
      841 tatctcccaa taaccatcga tacattgaaa taactcctcg tcatactctt caaaaattgg
      901 atagattctt aaaaaatcat caaatgataa gccttggtcc tcatagtaat ctttgactaa
      961 tccattttca attgcctgtt gggtcggtaa acccgtttca gtaataattc ctctctctac
     1021 catcgaacga taaaaagtgc atttatctag cgattgctgg gtcgtttcaa gataatactt
     1081 taatccttct tcaactaaag gtgggtaaat aatttccatt ttgattccct cctaaaagtt
     1141 atatattaaa ctattttgaa gctcctttaa ttactttctt tccattatag taccctttta
     1201 aagatacatg atggctacgt ctataatcac cattcgcttc gtcaaatgaa atttctggtt
     1261 ttactacagt ttgatgagtt cttcttaacc gtttcttcgc cttagatgtt tttcttgctg
     1321 gtactgccat gatatttcct ccttttacca acttgctttt tttactccag gaatcaaccc
     1381 ttgatgggca agttctctaa acttaatacg ggacataccg aactttcgca tataccctct
     1441 tggacgacca tccgtctgat cacgaagcct cagacgattc ggatttgaat ctcttggcaa
     1501 tttagcgaga gcggtgcgat ctcctgcggc ttttaattcc tgacgtttat ctgcatactt
     1561 ttcaatcatc gccatttgtt tctttgcttt agcaattttc gacttttttg ccatatacct
     1621 ttatactcct tttattttga tttagacacc tcaaatcgta atcgttacgt tttgacactg
     1681 atactttacc aacaagagtt gctcttgtca acaataaata acatgaaata aaactcatat
     1741 gaatagataa ctatttgaaa acgaatttat cggtacatag agataatcaa atcatttaaa
     1801 agaatatgca gtttaggctc tgaaaaataa gaattcactt ttattagtcc aatcaaacac
     1861 aaataccgtt ttaagtagct tttcctctac ttaaaacggc aatatgaaag aaactataac
     1921 tttttattca atcttttatg tttaactggt ttatcgccaa ttttttaaaa ctttgattat
     1981 tccttatatt ataagaatag acattaagtg aactaattca atcatactga ttctttcgtg
     2041 tattcaaaat tagatgctgt ttaataaaat tattcttttt gaaaaagaat tataactata
     2101 tcccaaaaat tgtaggttaa tttaaagcct atttcatcat cgtgattgag cttctttttc
     2161 atagacgaat ttctcagata gtttagtttt ctttatgaga tttttgtata tttctgacct
     2221 tcgatacaac tcattatgag taccttgatc aactagtttt ccttgatcca tcactacaat
     2281 tttatttacc ttttcaatag acttcaagcg atgcgatata attagtactg tcttatcttg
     2341 tattaaacga tttaaacttt cctgaatacg tttttcattt tccgcatcta aactggcagt
     2401 aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg
     2461 ttgtcgctct cctccagaaa gtgaacttcc gttttctccg accaatgtat gataaccgtc
     2521 aggtagtttt tcaataaagt cttcgcagtt agctaaacga gctgcttttt tcacttcctc
     2581 atctgacgct tgactatttc caatacgtat attttccaaa atacttgtat taaacaaagt
     2641 tacctcttga aaaacaatag cgattttgct aaacaaagaa gctggagata cacgtttaat
     2701 atcataccca tcaattaaaa tacagccttc atcataatca tacagtctag acactaattt
     2761 caagatacta gttttcccgg aaccactagc acctactaac gcagtcactt ctccttgttt
     2821 agccgtaaaa gagatatgat ctaaaattgg ggtattgtta tcatatgaga aagagacatc
     2881 tcgcaactca atgtcaaaag atttcagagg actatctgat ccctcttgga tactggtttc
     2941 cttcatagct ctaattcgct gtattttagg agctaaataa aatatttcaa gcactgcttc
     3001 tttcatagaa tcgaaggaat cctttatttt tattgcggct agtaagtaac caacgacgta
     3061 gagtatagtc acttcacccg ttaacagtaa gtgaacacct actagaatga ctactgctaa
     3121 actaacatat gagaaaatag aggataaggc catgactgaa aaagtactta attctacctt
     3181 taaatgaatc ctttcactct cttccatttt tttgaaaagt ctgtcttgaa cttttttaga
     3241 tagattaaag ctattaatct cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt
     3301 ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt tgggatttct ttgataacaa
     3361 gattaagaca aaactaaata acgttggcaa aataacagct aatcccattt tgacattacc
     3421 aaccaaaagc atcactgaaa taaaagggaa aaacagagcc ataccgccgg ctttaggtat
     3481 tgcatgactc atcgcatgct caataccttc aacgtcagac atgatagttt gagataaatc
     3541 tgataaatta tgttttgaaa aataggataa tggtaaattt gacaaatcgt ctgcaatttg
     3601 cactcttaaa tgcgcacttt ctttataagt actattatat aagttttcat actccctatc
     3661 taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg
     3721 ctttgtattt tctaaaacta attcgtctat cagcatcatt ataatgaatg ctggtaccat
     3781 attaatgcag tatacaaaaa aactagaaat agaagcttta gttaaagctt ctgcgccctg
     3841 atcacttaaa gcaaatttgt ttttataaaa acttttcatt gtcaagcctc caatcatttg
     3901 cattctcata attagtgacc aattgtttat acacttgatg tttagccatt aattcattat
     3961 gatttccacg ttctactatt ttcccttctg acaagacaag aatttcatct acagatttaa
     4021 tgctggataa tcgatgagct atcataataa ctgttttttc tttcatcaga tgttgaaaag
     4081 ccttttgtag ttcatactca ttatctggat caatagatgc actggcttca tccataataa
     4141 caatctttgc atcttttaaa attgctctgg caatagctat acgttgtttt tctcctcctg
     4201 acagataaac tcctttacta ccaattaagg tatgctctct ttcgggaaat ttttctaaaa
     4261 tcgattcgca accggctaat cttagcgcgt caaatacttt acttgctggc gcttgaggat
     4321 ttgctataga aacgttatcg taaatactct gattgaatag tttcgtatct tgaaagacaa
     4381 aagcaattgt gttaataatt gtgtcttttg aatagtgatt taaaggctta ccaccaattt
     4441 taatctgtcc cttatccaca ttgtagaaac ctgaaattag tttagctatc gtactctttc
     4501 cgcttcctga ttttcctact aatgcataaa ttcttttttg ttctaaaact aagttgaaat
     4561 tttctaaaac acactcttct ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat
     4621 ttttaatctg tgtctcgttc ccatatttta gttggtcttg cttcatttct aaataaaggg
     4681 agtctagtgt ttcgactgca tatcttgctt caaacaagtg catacttaca tacattattc
     4741 tcatgaaaga aacaaaaata attccactta aaaaaaacaa catcaacaag tcaacagcta
     4801 atatcttggg agtggtaaga gaagttataa aaaaggttat aggaactatc aaaatgcaaa
     4861 ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt tttcccactc aaagaatact
     4921 tatatgcaaa cttagcataa tcgttaattg ctttattcaa ggctttaaat gatgttactt
     4981 tagcaccaaa tatctttata acttgaattc cgcggatata ttctaccgtt tcagagctta
     5041 acacatctaa cgattcttga tatttttgaa taaaatttgt attctctgtc attttcttta
     5101 acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga
     5161 aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca
     5221 agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc
     5281 ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt
     5341 ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat
     5401 aaaataaagc tctaaagtta accaagcaac agtttgaacc gcataactct tagctaaata
     5461 ttcatctcct acaataatta gagactgaag aaatttgtaa atactgtaat atccatagac
     5521 tgttaatacg acagaaaaat cagaaaataa agtggctaac catccgtagt atttcatttt
     5581 agggacataa ctaaataatt ttttgtatac tttcattttt tcctcccccg cattcaattt
     5641 cttctcttat tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt agagatgtac
     5701 aaacagataa aaggtaaact acgttctatt ttttgtattt ttttaagact tttcttccaa
     5761 tcaatacacc aatcatagat aaacttgcta caactaacac tgaaatgatt ccccatatac
     5821 ctaccgcata gtggatcatt gcatccattg ttgcagcaga ttgtccttta tctatccaat
     5881 cttgacgaaa acgatctgta aagaaccata taggaatcca ctgtccggca gcactgccta
     5941 attgcattag agcccaagac gcaacttgag gaattgctcg atcatatcca aatacagata
     6001 gaataatatc agctaagatc gccatactaa tcgaactgat aaaccaaggt aagtacgccc
     6061 cgcccattaa cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa attccttttc
     6121 tagggtagtt atagctaata taaacaaata tgattccaga taataatcca aaaatcccgg
     6181 gggatatgct gtgaccaaac gccccaccaa tactactaac cattattgaa aaaaagtaaa
     6241 ttactaggga aataactgta gctaaaccaa taaaaatata atcttttgtt ttcatatata
     6301 ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc
     6361 tactctaagt tttatatctt tttttattcc accatttttt aaataaataa ttctattgca
     6421 tgttttataa agaaattccg gatcatgagt agctataact actgcaccct tttcttttaa
     6481 gtctttaata aggtcagcaa tcctttccat attaatataa tccaacccac ttgtaggttc
     6541 atcaaataga aataaattag tttttgacaa tgaagctata gaaaccaata atctttgttt
     6601 ttctccaccg gataaatcaa acggtaattg atctttcatt ttataaagtt ccattttttt
     6661 taaaattttt tcttgctgat ctttactaac tatttcattc cccagggcta actcgctcct
     6721 cactgattct gtgaaaaatt ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa
     6781 atttttttta ttttcgatta tctttccttt ggtagggcga ataatcttca tgagtattct
     6841 tagtaaggtt gttttcccca caccgttttt ccctaccaaa ccaataacat ctccaacttt
     6901 gacatcaaaa tttatgtctt ttagaatatt ttcata
//
LOCUS       DAZAOG010000136         6931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000136 DAZAOG010000000
VERSION     DAZAOG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.136"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHDLD_002279"
     CDS             complement(<1..182)
                     /locus_tag="ACHDLD_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3363961.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHDLD_002280"
     CDS             517..951
                     /locus_tag="ACHDLD_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3363962.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHDLD_002281"
     CDS             974..1294
                     /locus_tag="ACHDLD_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363963.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHDLD_002282"
     CDS             1435..1626
                     /locus_tag="ACHDLD_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363964.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHDLD_002283"
     CDS             complement(1697..1954)
                     /locus_tag="ACHDLD_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN3363965.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHDLD_002284"
     CDS             2112..2375
                     /locus_tag="ACHDLD_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363966.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHDLD_002285"
     CDS             2399..2587
                     /locus_tag="ACHDLD_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363967.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHDLD_002286"
     CDS             complement(2670..2855)
                     /locus_tag="ACHDLD_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363968.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHDLD_002287"
     CDS             3070..3441
                     /locus_tag="ACHDLD_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3363969.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHDLD_002288"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHDLD_002288"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHDLD_002289"
     CDS             complement(3709..4137)
                     /locus_tag="ACHDLD_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363970.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHDLD_002290"
     CDS             complement(4197..4955)
                     /locus_tag="ACHDLD_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN3363971.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHDLD_002291"
     CDS             5250..5447
                     /locus_tag="ACHDLD_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363972.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHDLD_002292"
     CDS             5553..6035
                     /locus_tag="ACHDLD_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363973.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHDLD_002293"
     CDS             complement(6093..6689)
                     /locus_tag="ACHDLD_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3363974.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2350 a         1064 c         1357 g         2160 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacagctgat atataattat taaatctcta t
//
LOCUS       DAZAOG010000137         6853 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000137 DAZAOG010000000
VERSION     DAZAOG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.137"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..1650
                     /locus_tag="ACHDLD_002294"
     CDS             97..1650
                     /locus_tag="ACHDLD_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN3363975.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2031..3815)
                     /locus_tag="ACHDLD_002295"
     CDS             complement(2031..3815)
                     /locus_tag="ACHDLD_002295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363976.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3830..5551)
                     /locus_tag="ACHDLD_002296"
     CDS             complement(3830..5551)
                     /locus_tag="ACHDLD_002296"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363977.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5651..6412
                     /locus_tag="ACHDLD_002297"
     CDS             5651..6412
                     /locus_tag="ACHDLD_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3363978.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2183 a         1215 c         1333 g         2122 t
ORIGIN      
        1 gagtacatac caaattcata aattttttat tagtgggact agtgattgta tggattttga
       61 cgcataatga atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc
      121 gaagtattat cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata
      181 atgttcaagc ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg
      241 ttcctatatc gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat
      301 aatttgtatc acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt
      361 cttctactta tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt
      421 caaagagcac aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat
      481 tttgagaaga cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg
      541 ctgagatcac ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg
      601 aacacattcc atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg
      661 cctggtggat acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt
      721 ttagctggaa caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat
      781 gctgcttctc acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa
      841 gggttgttag ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc
      901 aaacttgata cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta
      961 tttgtgccga ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg
     1021 attcctgaag gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt
     1081 atctattctt ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg
     1141 caaacgtcta ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat
     1201 ggcaacgatg gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac
     1261 atttacacta ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat
     1321 cagccagtac aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa
     1381 agtttgtttt atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg
     1441 gaaaactatc agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata
     1501 agcgcgaata cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg
     1561 aaaatgtttg cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag
     1621 caactatcgc aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc
     1681 accgagtcac aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact
     1741 cgtgattcgg agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt
     1801 tacaagatcg tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag
     1861 ggattgtgac aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa
     1921 gtatcattag tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta
     1981 tcgatcatgg actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc
     2041 tgaaattgac tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt
     2101 gttccttttt cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc
     2161 gttgacaaac ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct
     2221 tgctgaatca atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg
     2281 acaggaggat tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat
     2341 aacgtgccgt tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag
     2401 tgatcacatt gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc
     2461 ccgtaggcga tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc
     2521 ccaaaagaac gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg
     2581 atctttcctt tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct
     2641 gcaccagttg gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga
     2701 ttcatcaata ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct
     2761 ttcggttgtt ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat
     2821 atctcaaata tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa
     2881 gcggtagaaa ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg
     2941 atcgtcacac cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg
     3001 agttgattaa tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta
     3061 aaagcgttgt attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga
     3121 ttgaatgttt tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca
     3181 cccagttcat actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta
     3241 acaattgaac tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa
     3301 aacagcatca gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct
     3361 gtcaataaca cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct
     3421 acttgatgat tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt
     3481 cgcaccctca atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa
     3541 gtgatgctgc ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt
     3601 ttgatatcac tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca
     3661 tcgatcccga ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg
     3721 catagaaaca atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc
     3781 aaagcacgcg tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt
     3841 gtgattttgc aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt
     3901 gtccaacgat ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga
     3961 ggcgttgagc aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta
     4021 atgctgcatc tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg
     4081 ctggtttgat caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag
     4141 aaccgccttg agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta
     4201 attgagcgat cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct
     4261 gcaagttgct taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt
     4321 gggagcggat ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc
     4381 ctatcgtagg gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg
     4441 tcccgccaac gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa
     4501 ctggatcaga gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct
     4561 gatcaaaggt tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt
     4621 gcagtatttc ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca
     4681 tagctgcgat catcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga
     4741 caccgatttg taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat
     4801 aaacgattcc catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt
     4861 tgatcatttg attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt
     4921 gattatcaaa ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga
     4981 tccgatccat ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat
     5041 aaacaatcca tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat
     5101 aacgaaacat catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc
     5161 aaaataaaac aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag
     5221 atccagaact aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt
     5281 tctcccgcgt ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa
     5341 ctgcagcgat tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa
     5401 ctgaggcttc gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac
     5461 ctaatgtacc ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt
     5521 ttttctgtaa gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt
     5581 cgattctata ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag
     5641 gtgagaaaaa atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc
     5701 aaaatcaacg atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga
     5761 gaatgattat cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta
     5821 tagaaaatta gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga
     5881 gttatatgag acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa
     5941 acaaaaacaa aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa
     6001 cgaggaagaa tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga
     6061 agatattgtg gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat
     6121 gccagcttcc agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt
     6181 atttactcaa gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt
     6241 gaaatccgag atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta
     6301 tgaaaaagga tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga
     6361 aaacaatatc agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg
     6421 tgtttaagat aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg
     6481 attacgggtt taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga
     6541 ggaaatatgt ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg
     6601 tcctcttaaa aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa
     6661 ctgagactaa tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat
     6721 gataaattaa ataggtgcat aaaagcatta cacttaaaat acattgttgc ttacattatt
     6781 acaatatgtt aataaggcaa aaataaatag atggattgaa taaacatatt ttttaatcaa
     6841 atagtcccta ctg
//
LOCUS       DAZAOG010000138         6790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000138 DAZAOG010000000
VERSION     DAZAOG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.138"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..250)
                     /locus_tag="ACHDLD_002298"
                     /pseudo
     CDS             complement(47..250)
                     /locus_tag="ACHDLD_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            246..413
                     /locus_tag="ACHDLD_002299"
     CDS             246..413
                     /locus_tag="ACHDLD_002299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363979.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(456..1031)
                     /locus_tag="ACHDLD_002300"
     CDS             complement(456..1031)
                     /locus_tag="ACHDLD_002300"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN3363980.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1186..1712)
                     /locus_tag="ACHDLD_002301"
                     /pseudo
     CDS             complement(1186..1712)
                     /locus_tag="ACHDLD_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2169..3680
                     /gene="gadC"
                     /locus_tag="ACHDLD_002302"
     CDS             2169..3680
                     /gene="gadC"
                     /locus_tag="ACHDLD_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN3363981.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3693..5093
                     /locus_tag="ACHDLD_002303"
     CDS             3693..5093
                     /locus_tag="ACHDLD_002303"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN3363982.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5258..6698)
                     /locus_tag="ACHDLD_002304"
                     /pseudo
     CDS             complement(5258..6698)
                     /locus_tag="ACHDLD_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2102 a         1165 c         1223 g         2300 t
ORIGIN      
        1 gtcaataatt ttgtgtaaat aactcgtcct tctgcaaaat aatgggttac tcagtaaaca
       61 ttgaagctaa tgtatccgtc acttgttgga agcctttatg gcttctattt aagaattttt
      121 gattgtatgt atcaaagatg gatactagga aacgctctag tgattcttca ttttgaaact
      181 gctcttttct gcggctgtat ttcttgattt gtttattgaa agattcgatt gtagaaccag
      241 aaggaatgga gaacactttt gaactttaca acaaaactac tgttgataaa ttaattccaa
      301 ttttagttcc aactgattta aatggtcatg atgttttact tcgttttaac ggatttttag
      361 gtgaggatta tagataccta aaaatttctg atcctcaacc tttaacagaa taataaaaaa
      421 cgtgtaatca attatttgat tacacgtttt ttatttcaat cttcgttgta gtagtggttg
      481 cgacataccg aaaggtaatc ttcattacct cctactacaa tctgttcacc tgaataaact
      541 ggaataccat caacaatacg agcattcatt acagcctttt tattgcaaaa atggcaaata
      601 gttttaattt cctcaatttt atcagcaaat gtcaataatg cttcggatcc cttgaagaga
      661 ttattttgaa aatcattctt caatccatag caaattactg gtacttcctc attcattacg
      721 ataagtgata gatcaataat attctctcta cttaaaaatt gagactcatc gaccaaaata
      781 caatcaactt ttccgtactg atcaataatc tccactaatt ctccgtacac atccatttca
      841 gaagtaattg caatagctgg tttcgaaaca cctatacgac tctcaacttt tccaaatcct
      901 gatcgagtat ctattgcgct tgtcattatg ataactctct tcccttgttc ttcataatta
      961 tgagccactt tcagtagctc aatactcttt ccactattca ttgttccata tctgtaaaat
     1021 aattgtgcca ttaaatttat tcccttcttt tacaattcaa ttattcctta attccatctg
     1081 cttttatgca gctgctcgtt tattccttga tatgtctata acatctacta tccctaaaac
     1141 caatttataa gccattagag atggtattcc tgaacatctc tcatattgtt ttttgagtgg
     1201 tatatcatct gaaaaactga aggaatcatg aaaatagcga catacttatc atttagagtt
     1261 ctaactttaa atgcatttac ttcttcagtc agcactttag tcatgttgga catggtttgg
     1321 ggcgtgtagt gatgcccata cattttctca atcaagtctg cgatttccga catggtaata
     1381 ccttttcgga agaggtgaat gacggtctcc tctaatgtgt cattcgtccg tctataagca
     1441 ggaacagtct gttgcttgaa ctcgccattg cggtcgcgcg gaatctggag atgaagttcc
     1501 ccatactcgg tcttgaccgt acggtcatag gagccgttac gagaattacc cgtgttaaaa
     1561 ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta actccgtttt gagaagcgta
     1621 ttgacagctt tttccaaatg gacacggaat aatccattca aatcgccttt gttcgctaga
     1681 gtctttagga tttctgtagt aaaatcgttc atatggaagt cctcttttct gtgaatgtgt
     1741 tgtggtaact ctattctaca gaagggactt ccttttttct atttacacaa aatattttac
     1801 actctcaaca aaagtatctt cgattaatac ttgctcaagt tatgtgcagc tacgaaaaag
     1861 taggtaccga ggtactttct ttttcgcagc tttttttgga cttatgagaa gtacattcgc
     1921 tggaagaata cgatataaaa cgagcttttt cactaaagat attaatagag tgaaagaaac
     1981 aagcttttgt tgtcagtttt ttcacttttc taaaaatgaa aataaaaaat tctggaagtg
     2041 gagaagaatt ttgcttgcta tactttgtct tgtagaaaag aggtagacaa aataatgcaa
     2101 gctcaaataa gcgattttcc ataaccttgg taggaatgac aaattaaata ggaaagggaa
     2161 gtaataatat gaataaaaaa aagttatctc tattcggttt ttttgccctg acagcttcca
     2221 tggttttgac tgtttatgag tatccgacct ttgcaacatc acaattacat ttggttttct
     2281 ttcttctttt gggaggtatt ttatggtttc taccagttgc tttatgtgct gcagaaatgg
     2341 caacagttga aggatggaaa gatggtggga ttttcagttg ggttagtcaa actttaggag
     2401 aacgttttgg ttttgcagct atttttttcc aatggtttca aattacagta ggttttatta
     2461 caatgatcta cttcatacta ggagcgctgt cgtatgtaat taattttcca gagcttaata
     2521 cagatccatt tatcaagttt attggtgtat tagtcatttt ctgggtactg actttttcac
     2581 agttgggcgg gaccaatcgt accgctaaaa ttgctaaagc aggatttgtc attggaattg
     2641 ttattccttc gatcattctc tttggtttag cagcagccta ctttatcggc ggtaatccga
     2701 ttcaaatggc tattaatgcg aaagctttta ttcctgactt ttctcagctt tcaactttag
     2761 tagtatttgt atcttttata ttggcttata tgggcgtaga agcttcagca tcacatatta
     2821 atgagctgga aaatccccaa agaaactacc cattggcaat gtttttacta gtgattttgg
     2881 ccatcgcctt agatgcaatc ggcggtttct cagtagcagc ggttattcca caacatgact
     2941 tgtctctcag tgctggtgta atccaaacat ttgaaacgct tattttacat tttaacagtc
     3001 atttacattg gttggtaaaa gtgattgctc tcatgattgc ctgtggcgtt atgggtgaag
     3061 ttagttcttg ggtagttggt ccatcacgag ggatgtttgc agctgctcag cgaggtctgt
     3121 taccagtttt cttccggaaa actaataaac aagacgttcc agttccgttg attatgatac
     3181 aaggaattat cgttactatt tgggcagcag tcttaacttt tggtggagga ggcaacaatt
     3241 tatcattctt agtagcaatc tccttaactg ttgtcattta tctcgtagga tatttactat
     3301 ttttcctagg ttactttgta ttagttttta agaaaaaaga cttaaaacga acttacaatg
     3361 taccaggaaa aactgttggc aaaacgatta tcggtggaat tggttttatt ttatcagtgt
     3421 tcgcattgct tatctctttt gtaccaccag cttcaattgc agctagtgag caccgaacat
     3481 accaaatcat actacttatt agctttgtag tgactgttct tttacctttt atcatttatg
     3541 aagtacatga taaaagtgct cacacaacta ttgaggaacc tactcacgtg aaagctgaag
     3601 acgttaatcc cgctgtttat ccagctgcta gaggagagca ccacgttaca aaaaatgatg
     3661 aacatatttt aaaacattag gaggaattca ttatgttata cggaaaagat aatcaagaag
     3721 aaaaaaacta tttggaacca atttttggct ctgcaagtga ggatgttgac ttgcctaaat
     3781 ataagttaaa caaagaatcc attgaaccac gaattgctta tcaattagta caagacgaga
     3841 tgttggatga aggaaatgcg cgattaaact tagctacttt ttgtcaaacg tatatggaac
     3901 ctgaagcagt gaaattgatg acccaaacgt tagaaaaaaa tgcaattgat aaatcagaat
     3961 acccacgaac aacggaaatt gaaaaccgct gtgtaaatat gattgctgat ttatggcatg
     4021 ctccaaataa tgaaaaattc atgggaactt caacgatcgg ctcttcagaa gcctgcatgc
     4081 tgggtggtat ggccatgaaa tttgcttggc gtaaacgtgc tgaaaaatta ggtcttgata
     4141 ttcaagcaaa aaaacctaac ctggtgatct cttctggtta ccaagtttgt tgggaaaaat
     4201 tctgtgtata ttgggatgtg gaactgagag aagtcccaat ggatgaaaaa catatgtcaa
     4261 ttaatctaga tactgtcatg gattatgtgg atgagtacac aattggtatt gtaggtatta
     4321 tgggtattac ttacactggt cgttatgatg atatcaaggg tctgaatgat ttagttgaag
     4381 ctcacaataa acaaactgac tataaagtat acattcatgt tgacgctgca tcgggtggct
     4441 tttatgcacc atttactgaa cctgatctag tttgggattt tcaattgaaa aatgttatct
     4501 caattaattc ttcaggtcac aaatatggtt tggtatatcc aggtgtgggt tgggtcttat
     4561 ggcgtgacca acaatactta ccagaagaat tagtatttaa agttagttac ttaggtggag
     4621 aaatgccaac tatggctatc aacttctctc atagtgcagc acaactaatt gggcaatact
     4681 acaactttgt acgctatggc tttgatggtt atcgtgatat tcaccaaaga actcatgatg
     4741 ttgctgttta tttagccaaa gagatcgaaa aaactggtat ttttgaaatc attaatgatg
     4801 gatcagaatt gccagttgtg tgctataagc tgaaagaaga tcccaatcgc gaatggacac
     4861 tatatgattt atctgatcgt ctgttaatga agggatggca agtcccagcc tacccattac
     4921 ctaaagactt ggatcaatta attattcaac gcttagttgt tcgagcagac tttggaatga
     4981 acatggctgg tgattatgta caagatatga accaagcaat tgaagagttg aataaagctc
     5041 atattgttta tcataaaaaa caggatgtga aaacgtacgg ctttactcac taaaattctt
     5101 cagagaaata taaaagaagt taattaacta ataaaaggag ttgtgattac cacacagctc
     5161 cttaaatcta tttgaataaa tatatgatat tcatttgtta cactctcaat cttagctgtt
     5221 gttctattca tcattactct tattgtaagt agttcagtca aatttgcttg ttcaattgat
     5281 cgataacagt atgaattgtt tgaaaagtaa tcggatatat aattgttttc tcactaccta
     5341 agccttcttg gtataaagaa ctatcgcaaa gtataatccg ctgagtacaa aagaaagtgt
     5401 cgtctactat ttctctaagt tgtttacaaa tgatattact aatagaaaag tgttctccca
     5461 aaatgtcttc taactgttgt cgatagtata aatacctaaa gtctgacgga gctactatga
     5521 acacttcaat tggagatttc cgtttatgct catttaaaag acttaaacta atggttagtt
     5581 tacgaataga attctcattc caacgcagaa ataaattgtt ttgttttcgc cattgttcaa
     5641 gaatttcatg aacttctaag tacaaagcgc gttgctcttc atttaacaag taagtcttat
     5701 caggttgaaa tatctgaata tcagcccaaa gtgagcggat aaacgggaga agtgctttat
     5761 caaaaagtat ctgattttct tcagatatat tcagagataa gataagtttt tgactgagct
     5821 caatcaccaa agggttatct gctacatggt tttgataaac aacgttcaag tctttttgta
     5881 acaattcaaa gttatcgcat agataatttc tagagttgaa tagaaagtag atataaaggc
     5941 attcatcttc gtcatgacat aaagagaaag aaagttgctc taatcctttc tgtattagct
     6001 gaaacaaggg agtatgtcgg gcaactcttt tttcttcctg actaaaatat attgggttag
     6061 tcttagatct ctgcaaaccg atagcgatac ctaataaaat aaattgtttt tcaactgtag
     6121 ggtaacttct tgaaaaaaaa tgtttttcta cataatcaat caattgttga ccgctttgaa
     6181 taattgattt atttagctgg atcaactctg tccgatttgt ctcatagatt atcgttagta
     6241 acaaacttcg ataatccttt tcaggaattt caaaatggcc atcagcattt aagtactgca
     6301 tttgtttaaa aaaattaatc aactcatttc gtaagttgta aactttgctt aacgaaatga
     6361 aggtagtatc agctatatta ctccaattgt gttcccctga taaataatgt cctagtacat
     6421 tgaaaaagtc agagcgctga tagatgagtt gagtgagtgc caacctcgaa aacgaagagt
     6481 cgaatgcgac tgtaaagcta cttctagatt tctccactga aataatttgc tgatggagta
     6541 tgtcggtaat gcattccaag tcgttagtga cagacagtga gtgggcaaac gttcaattga
     6601 tctgctattt cgcgaatctg aatggtccct ttttcgtgta attgctcaca aatgtaaact
     6661 tgacgaaaaa tacttttctc cagataggtt tccaacatag tactcctcct agtcatttta
     6721 tacataacta ttataactct accaatcgga atagtcctgt aatatattga atcaaaaata
     6781 taaattataa
//
LOCUS       DAZAOG010000139         6570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000139 DAZAOG010000000
VERSION     DAZAOG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.139"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHDLD_002305"
     CDS             127..579
                     /locus_tag="ACHDLD_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363983.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHDLD_002306"
     CDS             593..1282
                     /locus_tag="ACHDLD_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363984.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHDLD_002307"
     CDS             1407..3758
                     /locus_tag="ACHDLD_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN3363985.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHDLD_002308"
     CDS             3838..4233
                     /locus_tag="ACHDLD_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3363986.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACHDLD_002309"
     CDS             4558..5175
                     /locus_tag="ACHDLD_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3363987.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACHDLD_002310"
     CDS             5216..5515
                     /locus_tag="ACHDLD_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFN3363988.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACHDLD_002311"
     CDS             5549..5716
                     /locus_tag="ACHDLD_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN3363989.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACHDLD_002312"
     CDS             complement(5761..6366)
                     /locus_tag="ACHDLD_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3363990.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAZAOG010000140         6406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000140 DAZAOG010000000
VERSION     DAZAOG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.140"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            395..1363
                     /locus_tag="ACHDLD_002313"
     CDS             395..1363
                     /locus_tag="ACHDLD_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3363991.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1381..2286
                     /locus_tag="ACHDLD_002314"
     CDS             1381..2286
                     /locus_tag="ACHDLD_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3363992.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2283..3089
                     /locus_tag="ACHDLD_002315"
     CDS             2283..3089
                     /locus_tag="ACHDLD_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3363993.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3246..4214
                     /locus_tag="ACHDLD_002316"
     CDS             3246..4214
                     /locus_tag="ACHDLD_002316"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN3363994.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4531..5055)
                     /locus_tag="ACHDLD_002317"
     CDS             complement(4531..5055)
                     /locus_tag="ACHDLD_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN3363995.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            5342..>6406
                     /gene="scm"
                     /locus_tag="ACHDLD_002318"
     CDS             5342..>6406
                     /gene="scm"
                     /locus_tag="ACHDLD_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN3363996.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         2026 a         1304 c         1264 g         1812 t
ORIGIN      
        1 tgttttcaat tatctgacaa ttcttaattg acagaccttt ttatctcggt tatactttag
       61 aaaattcatt ttgtggagga acttctatgc gaaccaacat caaccaaatc gcaccaacac
      121 aactgaataa cttttacttt agctacttta gataggtttg tattcatcat gatggataca
      181 aacgttaaac gacagtttgt atctatgatg gctaaagaac tttgtattgc agcaaaaagc
      241 cacgtagaca aaatgctaag tggctgagca gtgaaaatac aaagtaaaag tatcaagcat
      301 tctacttttt caatttattt tccgactttt caatccattt ggacattttg tctgaatgga
      361 ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa tcatcggatt
      421 aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa aaagacaatt ccgcagaggc
      481 atcagatgga caaaaaacaa ttggcgtatt gcaacttgta caacataact cgttagatgc
      541 tgcctataaa ggattcaaag aaggattagc agaagaaggg tataaagaag gcgataatct
      601 aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta tgagtgagaa
      661 actcgtcaaa gaagatcctg atttgctact tggaatcgcc actccagcag cacaatcttt
      721 attaaatgaa acaaccacta tcccgatgac tgtcacagcg gtcacagact tggaacaagc
      781 ccatttagtc acatccaatg aaaaacctgg cggcaatgtg actggcacga cagatatggt
      841 gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca aaacaatcgg
      901 catcatgtat aacaacggtg aagcaaactc taagatccaa gctgatttgg cagaaaaaga
      961 attgaaaaaa gcgggcgtca aagtcaaagt actgacagca aattccacaa atgatgtcca
     1021 acaagtgacg aaaagtctag ctaaagatgt agatggaatc tatattccta ctgataacac
     1081 gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa cacctgtcgt
     1141 tgctggcgca gttgatcaag tcaaagacgg cggtctagca acagtaggaa tcgactacga
     1201 aagcttgggc aaacaaacag ggatcatggc agcaaaaatc ttagacggca agaatccaag
     1261 tgaacttcct gtcgaaacag cgaatgattt ggaactgtat gtcaataaag acatggccaa
     1321 agcattaggc atcaatccag acagcatcaa agaagctgaa taaaacacag gaggattctc
     1381 atgacaaatt tgctctctct tttcttatca tctgcatcgc aaggggtctt atgggcccta
     1441 cttgcgattg gtgtatacgt cactttccgt atcttggaca ttgccgactt gactgctgaa
     1501 ggaagctttc ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg ctatcatcca
     1561 ttgacagctt gtcttctagg ctttttaggg ggtgcggcag cagggcttgt ttccggactg
     1621 cttcacacaa aattgaaaat tcccgccctt cttgcaggga tcatcaccat gactggttta
     1681 tattctgtca catcccgtgt gatgggtgcg ccgaacatct ctttattggg tcagccaacg
     1741 atctttcatt gggtcgagtc attcggcata acaaaagaca acgctgtact gatcgtcggt
     1801 ctgctttttg ctttgatcgt tgtatgttta cttgttttgt tcttacgtac agaaacagga
     1861 cttgctattc gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg gatcaacaca
     1921 gataatatga agatcatcgg ttatatgatt tctaatggat gcattgctct ttctggctcc
     1981 ttacttgcgc aaaacaacgg ctttgctgat ttaaattctg gtattggaac gatcgtaatt
     2041 ggacttgctt ctattatcat cgcagaagtt ttgttacgta accagccact atttctccgt
     2101 ctcttgactg tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt agtcttcgaa
     2161 ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt gatctgtctt
     2221 tctttcccgc agatccagca aaaattccat ttacctcaaa aaaagaaagg cggtagtgtc
     2281 aaatgaatga cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa ggaacgatca
     2341 acgaaaatca tgtcctgaaa ggcattaatc tttccttaga tcccggcgac ttcgtgacga
     2401 tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc ggtacatttt
     2461 ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa tctgttgtcg
     2521 aacgttcgaa atccatcagt cgcgtattcc aagatcctaa actagggaca gccgttcgtt
     2581 taacggtaga agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc ggcttcttcc
     2641 gtggtgtcaa accacaagac cgaacatttt tcaaagacca gctggttcgg ctaaatttgg
     2701 ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa cgtcaagcta
     2761 ttacgttatt gatggccaca ttacagcgtc cagaactgat cttattagat gaacacactg
     2821 cagcgcttga ccctgctact tccaaaacgg tgatggaatt gacagaaaag ctgatcactg
     2881 agcagaatct cactgctttt atggtcactc atgacatgga agacgcgatc cgctatggga
     2941 gtcgactgat catgctgcat caaggaagga tcgtcgtaga tatccagcaa gaggaaaaac
     3001 agcagctcac agtgccccaa ttattagcgt tgttcaaaca aaacagcggc acggcattaa
     3061 aagacgatca agtactttta tcttcataat cagaaagagg atgggacaaa aaatgaacgc
     3121 ccaccctctt cttttttctc tttccttctt tcgtttttgc tttatgtatc aaccgttgtt
     3181 tcttcgaaaa ctattttgtt ctctccttct tcctgataga atggatggga aaggagcttg
     3241 atgccatgac aacgaaagac aaggtacttc aattattgaa aaagacacca gattttcttt
     3301 ccggagaaaa gttggcacag caactggaag tttcccggac aagtatttgg aaagccatca
     3361 aagaattgga aaaggcaggc tatcgttttg agcacgggcc tacagggtat cgttacctgc
     3421 cctctgacgt actagatgca aaagaaatcc agcaagagct acggtcgttt attcctgatt
     3481 tgaaagtaca aacgatgttg acttctgaat ctacgatgaa agatgccaaa ctggcagcag
     3541 ctcaaggaga ttctgtttcg aagttatttg ttgctgatac acaagaacaa gctcgtggac
     3601 gttttggacg gccttttttc gctgaacctg gacgaggaat ctacatgagt ctcttgctcc
     3661 atccagacaa acggtttgat gaattgcctc aatatacgat catcgcagcc agtgcctgtg
     3721 tgaaagccat tgaatctgta accggtcgtt ctgcttcgat caaatgggtc aatgatatct
     3781 acatggatgg aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat atggaaagcg
     3841 gcaggatcag tcatatcatc attgggatcg ggcttaattt ttctgttcct caaaatcatt
     3901 ttccagctga tctccaagaa aaagccatct ctgtctttcc cgacggacaa gctagtgtca
     3961 caaggaatca gctaatcagc aaaatctggc aagaattgtt cacttatctt tctcacccag
     4021 aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt gggaaaaacc gtgtcgttca
     4081 atcaaaaagg aacgacttat gtcggtcttg caaaagaaat cggtgatcat ggcgaactga
     4141 tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt ctcacatcta
     4201 tccgtacgca ataattatca gatttcacaa aaggacacct gatctcatac agtgtccttt
     4261 tttctgaatt ctcttcaaaa gactctcctt attctatcag ctgacagttt ctctatcctc
     4321 tagaaaaagg tattctaaaa atatctataa agcatggtac actttcttta aatgaatcaa
     4381 tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag agatagcagg ccgcttcctt
     4441 tttcaatctt atagatagac catgggtaac gataaaaaca aaaaagaact tgcgacttat
     4501 tttgccgtaa gttctttttt agctagtgcc ttaatctctt aatcccaaca aacgtctcgc
     4561 atagatcacc tgaccagagt gcatagcagc atcatcaata atagaaacga gacgcattcc
     4621 tcgtgtgact ggtggtgtcc agttctcgtc aattacttca tctagctcgg aaggttccag
     4681 ccttcttaag taagaaatag caaaatcagc agctcctcgc aagtatccta gcagatcttc
     4741 tatttgatta gcttggatct gctttgcttg ttccagacta tgcttccaat cttgttcgtt
     4801 atctgctatc gtcatcggaa accggctttc ccagtcttga ctatgccaaa ttggagaagt
     4861 tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag ccagccaagt
     4921 cattgatttg atctgcgggg ctctatcttc cactggaaaa cgattggcct cctcaataga
     4981 gacgccttca aacaaacgtt caaaccgatc gtatgcccga tccacactct cgcatagcat
     5041 atctaaataa tccatttatt ttcctccttt ttatttatcc tacttacttt ttcctaattc
     5101 taggatacct acttatatgt tactacacac ccttattttt tacactttaa cagtcaccat
     5161 catcaatagt ttggagattg aaagaaaaag atagtgtcga actatcaatt gaacaagaag
     5221 caacaatatg aacgtttcat taatattctt taaataaaca atgatttttc aaacgaacag
     5281 tttaagacta ggagaaaaag agtataatag gaaaatgttt gaaaggaaag gaaggggaaa
     5341 aatgaaaaaa agggcaagtt tactagtcct agttgctttg ttgttcaata tcttttcgta
     5401 tggtgtggcc gtttcagcgg aaacatacat tcctaaagac cctgggaata tttataatga
     5461 tctaactggt aactatggct tgttaggaat tgctagtcaa ttccacgtat ttgctaaaaa
     5521 taagacaact gtgaatgcac atacagacgg aaatatagca acacatgaac ttgatgcaaa
     5581 aaataatttt ggtacagagc ttcatgaagg tttattgaac caagaaataa attatataca
     5641 aaaaactgat tctttcgcta attcttcagg catacctaca ggtagcacaa gaatgaataa
     5701 atttgtcgtt ggcagtaacg tggatgtagc acttcaagat ggtcatccta cagtcaatgg
     5761 atccaggctt gatcacttaa ctccagatga tttttataaa gatcgtgctg ggcatgaata
     5821 tattgatttc gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc ttgcaacgat
     5881 cactccagct aaaacatata ctgctgcaga ttttccagac atgaacaatc ggacaatcga
     5941 tttaactgat ttatcagata ccgggttttt attggtaaat atcgatgcag aagtgttaac
     6001 gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat ttaatgtcat
     6061 caacagttct tctgcattga acgtacagtc accaatcaaa tacaatgatc gttcaaatca
     6121 tgaaacagaa gatttttcgg atgcgaatat ctcttggaat tttggaaatg agatgactga
     6181 tttgacaatc agtgctcctt ttcaaggaac aatccttgcg cctaatgcta ctatccgcat
     6241 tactcaaaac caagacggaa cgattatagg aacaaatgtg attttagatg cagctaccaa
     6301 tcgttgggat cctaacgaaa tatttatcag cgacaatggt acagatacga cagacacaag
     6361 cgacagtaca gatacatcta ccacaagtga tagctcagat tcatca
//
LOCUS       DAZAOG010000141         6341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000141 DAZAOG010000000
VERSION     DAZAOG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.141"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..287
                     /locus_tag="ACHDLD_002319"
     CDS             78..287
                     /locus_tag="ACHDLD_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3363997.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            280..429
                     /locus_tag="ACHDLD_002320"
     CDS             280..429
                     /locus_tag="ACHDLD_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN3363998.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            583..1248
                     /locus_tag="ACHDLD_002321"
     CDS             583..1248
                     /locus_tag="ACHDLD_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3363999.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1532..2617)
                     /gene="xerS"
                     /locus_tag="ACHDLD_002322"
     CDS             complement(1532..2617)
                     /gene="xerS"
                     /locus_tag="ACHDLD_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN3364000.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2951..3184
                     /locus_tag="ACHDLD_002323"
     CDS             2951..3184
                     /locus_tag="ACHDLD_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN3364001.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            3181..3588
                     /locus_tag="ACHDLD_002324"
     CDS             3181..3588
                     /locus_tag="ACHDLD_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN3364002.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4725..5243)
                     /locus_tag="ACHDLD_002325"
     CDS             complement(4725..5243)
                     /locus_tag="ACHDLD_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN3364003.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            5610..5858
                     /locus_tag="ACHDLD_002326"
     CDS             5610..5858
                     /locus_tag="ACHDLD_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364004.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         2171 a          980 c         1034 g         2156 t
ORIGIN      
        1 tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata
       61 aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg
      121 ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct
      181 atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt
      241 taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat
      301 aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga
      361 gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact
      421 tgtttctaat tatggaaaag aagcagaaac ttctcaaagt actatgttgt ttgaggagtt
      481 tttttataaa ttattcctac cttattatca agggaaagta gaagaaagaa catataaaaa
      541 tcggttatct acaatcaaaa aaacttttct tatttcttta aaatggagtt aagagaaata
      601 cgtccaattg acattcaaaa gtggcaaaat aatttattga gcaaatataa aaattcttat
      661 gtaagaaaca tgtatgggat ttttcagatg tcattagatc gagcagttgt tttgggacta
      721 cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga aaaggaaaac tacagttgat
      781 ttttggacaa aagaagaatt tacaaaagtt atttcaactt tttatattga tgatttgtac
      841 caacattttg gttttatttg tatgtggtta ctttttatga ccgttatgcg tttgggagaa
      901 gccactgcat taacttggaa agatattgat tttgaaaata aaaacttgcg tgtgaataaa
      961 acactctatt ataagagagc caaaacctat gagtttgtag aacccgaaac ggcttctagt
     1021 attcgtacaa tcgaactaga cgacaataca attacctttt taaaagcttg gaaggagcgt
     1081 caagtttcaa taggaggcat gtcgtttgta ctttcttata atggaacacc tactcagaaa
     1141 tatacaatcc gtcatattat gagaagacat gcaacgatcg ttggtgttca tataatccgc
     1201 atacacggat taaggcatag tcatgcttct ttgcttatat cacaataaaa agatgcactt
     1261 cgaattaaag aaaggttaaa acatgctgat gtacaaacga ctttaggaac ttatggtcat
     1321 ttgtatctaa attcattgag agagataaca aatgatccca cagattatat ccaaactaat
     1381 ttttctaaaa tcaaaccgac acctatttac gataataaaa aataaatgat aaacaaaagt
     1441 aggatcacac caaaagaaac acactagaaa aactataata caggcttttc tagcgtgttg
     1501 tttcttattt ccgcctattt atacattata ctcataaagc atttaatgca tcgatcgtag
     1561 ccgcattgtc tatatgggta tataaagttg ttgtattggt acttgaatgt cccaattgtt
     1621 gggcagttaa taccaatgaa tttgtctgtt ggtagagtct agtagctaat gtatgacgca
     1681 gtttgtgagg gctgacttga attctaaagc cttttgaata ttttgctacc atccgttcga
     1741 ttgcaatacc ggatatacgc ttggcttctc cttggtaaat tgctagaaat aaagcttttt
     1801 cgttttctgt tactttatat cgttctttcc gaatagctag ataattagct aaatattcca
     1861 ttgcgaatgc tgctatattg acagcatctc ttttatttcc ttttctagtt actaccacat
     1921 tattcttatc taaagatagg tcatctaaat taatatttat aacctcggag agtctcaatc
     1981 cagatcctaa aatcagtgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
     2041 tcaaagctcg tttactcaaa gtatttttat attcattggc aatataatct agataatttt
     2101 gtgtatcctt atctagaaat aattttttct caatggcatt ggcacgtgca gctaacgtag
     2161 ttttatctgt gatggttgct acttgatcca taggattatt gtctaagtat ggttttccta
     2221 gaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgcc gtgatatttc
     2281 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
     2341 tctctttttt tacattttct tttgtgcgac gtttcaaatg agatttgtac aagtttaggt
     2401 cataggcagt taactgtctt atcgaggtga tagaagtatc aggaacgata gactcactca
     2461 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtggtt cgaggtgatt
     2521 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     2581 ttgtatctat tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta
     2641 ggggagaaga gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt
     2701 atgctctaaa atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata
     2761 atttaaaata aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt
     2821 ttgaattttc ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga
     2881 gggactatgc ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag
     2941 ggagaagact atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt
     3001 aactatttca aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt
     3061 agatgaagaa aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga
     3121 tttagcaata tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa
     3181 atgagtattc attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa
     3241 aaatactctc ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta
     3301 gggcaacttc gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca
     3361 gctatcctac ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact
     3421 gctttcttag ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa
     3481 aaagatgctg taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta
     3541 aaatatgaag tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta
     3601 tgtgtcttta tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc
     3661 tataattcaa atccaactat atagaaaacg taaattttct atatagttgg attaagataa
     3721 cgactattta ttttatcata tttttactat accctatact tttacttggt atatttatga
     3781 ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa aaatactaaa
     3841 gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca
     3901 cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa
     3961 gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc
     4021 ttgcagaaat cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt
     4081 tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt
     4141 caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag
     4201 gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt
     4261 agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca
     4321 atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc
     4381 atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa
     4441 aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga
     4501 tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa
     4561 attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac
     4621 ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat
     4681 acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc
     4741 ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata
     4801 aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt
     4861 gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta
     4921 cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac
     4981 ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat
     5041 ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct
     5101 aaattttcag caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga
     5161 atagtttcaa attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct
     5221 tctttatttt tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa
     5281 ttcatctata atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca
     5341 ttgactgtct aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa
     5401 atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg
     5461 actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac
     5521 taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt
     5581 cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat
     5641 tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg
     5701 acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac
     5761 tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta
     5821 ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg
     5881 cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg
     5941 taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata
     6001 attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat
     6061 tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg
     6121 ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg
     6181 gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct
     6241 gctcatttca acttatatgt tttagtgaac tacccccact tagcttggat aggctacaat
     6301 taactagaca gaaaaattaa ggtgtgtaga ctagaagaaa a
//
LOCUS       DAZAOG010000142         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000142 DAZAOG010000000
VERSION     DAZAOG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.142"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHDLD_002327"
     CDS             334..714
                     /locus_tag="ACHDLD_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN3364005.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHDLD_002328"
     CDS             696..1610
                     /locus_tag="ACHDLD_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3364006.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHDLD_002329"
     CDS             1855..2544
                     /locus_tag="ACHDLD_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364007.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHDLD_002330"
     CDS             complement(2665..2751)
                     /locus_tag="ACHDLD_002330"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3364008.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHDLD_002331"
     CDS             3427..4377
                     /locus_tag="ACHDLD_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN3364009.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHDLD_002332"
     CDS             4398..4931
                     /locus_tag="ACHDLD_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3364010.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHDLD_002333"
     CDS             4947..5117
                     /locus_tag="ACHDLD_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364011.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHDLD_002334"
     CDS             5567..6100
                     /locus_tag="ACHDLD_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364012.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHDLD_002335"
     CDS             6112..>6303
                     /locus_tag="ACHDLD_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364013.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZAOG010000143         6259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000143 DAZAOG010000000
VERSION     DAZAOG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.143"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1747)
                     /locus_tag="ACHDLD_002336"
     CDS             complement(<1..1747)
                     /locus_tag="ACHDLD_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3364014.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDW"
     gene            complement(1959..2855)
                     /locus_tag="ACHDLD_002337"
     CDS             complement(1959..2855)
                     /locus_tag="ACHDLD_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3364015.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2848..3528)
                     /locus_tag="ACHDLD_002338"
     CDS             complement(2848..3528)
                     /locus_tag="ACHDLD_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3364016.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(3529..4311)
                     /locus_tag="ACHDLD_002339"
     CDS             complement(3529..4311)
                     /locus_tag="ACHDLD_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3364017.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(4304..5221)
                     /locus_tag="ACHDLD_002340"
     CDS             complement(4304..5221)
                     /locus_tag="ACHDLD_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3364018.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(5385..5639)
                     /locus_tag="ACHDLD_002341"
     CDS             complement(5385..5639)
                     /locus_tag="ACHDLD_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364019.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1846 a         1282 c         1016 g         2115 t
ORIGIN      
        1 cccaatcaat tccttctgga ttaacgtaga cacagattac tggctttttt ataaaatcta
       61 cttcgatatg cggcatagca aaatcattca tcgctgttga agatagtttt tctcttttga
      121 ttgctccctc tagtatccct tctttcgcgt aattttcttt taccaatttt ttggtaatta
      181 tatttaaaac gttcacttga ttatccttta tttggtgggt aaaaaagcaa aatcgtttat
      241 ctaataaatt tagaaaagca tcttctgttt gttcatttaa ctgtagatac tgttttactt
      301 ttctgacatc tgcccctaac atcaaaggac taacaacaat aacaggaaaa tttgtatttt
      361 caatcggaat cgtgcttata acaagttcat cagtgacttt tctatcttgt agttcattga
      421 tgcttagtac atcttctatc tcaaccatat ctgcaaaacc gttatttatt ctttgctcta
      481 ataattttga tgttccatat ccgctactac aaactaatat cactttcgtt ttgtcaggtt
      541 tgtaacgatc caaaagtgat tgaatatgga gtgttacgta agcgatttca tcttcattaa
      601 aatgtataca gaatctctct tcgatttcct ctaataaatc tacacttatc ataaatgacc
      661 ttttaaaaga atatttgatt ttgtccgtat aagggttata gatatttact cccaatttga
      721 gtcttttaac tgctgcattg agatgtaata ctagattatt gatcaattct tcatcaggat
      781 aaaattcatt taatttaatt cttactaact cttgtagctc aacacccata tctaaatagg
      841 aaagcttcgt ttgatctaag tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa
      901 tgtcaacata agcttgttca tctattggca caataattga aaatgctttt tcaatcattt
      961 tgattaaaat aagtgtattc tttgagattt cttcttcaga attattacta cgtgataaga
     1021 aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt
     1081 cataatctgt aaatttcaat tgtgtcttat tcatgtatcg attaagcaat tcggctactt
     1141 ttttcaatat gtccttattt aagaaattat tcgcttcacc aatgaaattg atattcaagt
     1201 ctaagagatc tgttttactg ttagatgctg taaagccacc ccaataatag ttcacaactt
     1261 ctgacatcaa ccttctttta cttttttcat cagctttgat gcgcacgcca tttcgatctg
     1321 aaactaagga catcccatgc ttgtttagtc tttttctaac atctttcatt gtgctttcta
     1381 ccgtcgacct gctcacgtat aattcgtcag caagattttg aatctttaga gaatcattag
     1441 tattcaataa tttagcaaac acataagtta gacggtcctc cggagagtct gcgatattga
     1501 ttttatgact ggataataaa tttcgaagtg tttctttttt tccaataagc gaaacgcctt
     1561 ttttaggttg aaccaacatt tgtacatctg gcattttttg gattatttct ttcaaaatta
     1621 aaatatcttt gctaattgtt ttcgtagata catataattg ttctgataat tctttgtaag
     1681 ttgtgtgctc attttcaatc aaaaagtata gtattttcat ttgccgttcg ctaaattcga
     1741 tgcccattat cttcacctcc tgaatacagc ttaagtgtac aattattatc tctattttca
     1801 tacagaagaa agttcttgat taatgtagaa gtttctaata ttcttgatta tttcaaatca
     1861 ataaaaaagc ctgcaatatt tatatcacag gcttttttct ctattaaata tatataagat
     1921 aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg
     1981 gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc
     2041 aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt
     2101 tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga
     2161 ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca
     2221 tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga
     2281 tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca
     2341 gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta
     2401 tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc
     2461 atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc
     2521 gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg
     2581 agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct
     2641 tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag gtccattaga
     2701 ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg
     2761 gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata
     2821 aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt
     2881 atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt
     2941 cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg
     3001 cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt
     3061 caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc
     3121 cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg
     3181 gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc
     3241 tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat
     3301 cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag
     3361 catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt
     3421 acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg
     3481 aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc
     3541 agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac
     3601 cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag
     3661 ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc
     3721 gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact
     3781 tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa
     3841 tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa
     3901 tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt
     3961 tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa
     4021 agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa
     4081 tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc
     4141 accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata
     4201 ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat
     4261 tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc
     4321 ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca
     4381 caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa
     4441 aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa
     4501 ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg
     4561 ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag
     4621 tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt
     4681 cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat
     4741 tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta
     4801 aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc
     4861 gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga
     4921 tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac
     4981 ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat
     5041 caatcgagat caaacccata atagttctca taaaggtaga cttacccgct ccattttccc
     5101 caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg
     5161 cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca
     5221 tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc
     5281 cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca
     5341 atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct
     5401 ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc
     5461 agatcaagct tgagtaattt taccagaccg gttataagga taactatagc aagactccct
     5521 cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca
     5581 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata
     5641 agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
     5701 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
     5761 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
     5821 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
     5881 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
     5941 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
     6001 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
     6061 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
     6121 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
     6181 aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag
     6241 tttgaaaaac cttgattta
//
LOCUS       DAZAOG010000144         6255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000144 DAZAOG010000000
VERSION     DAZAOG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.144"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2446
                     /locus_tag="ACHDLD_002342"
     CDS             <1..2446
                     /locus_tag="ACHDLD_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN3364020.1"
                     /translation="KHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVE
                     RYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDI
                     DTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENS
                     MMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRK
                     HTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEY
                     YFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEI
                     EGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMI
                     FQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVL
                     GYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLA
                     SNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSS
                     EERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFE
                     QGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQ
                     TVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQK
                     NGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLP
                     TYEILLPSSLKSYFRL"
     gene            2628..2867
                     /locus_tag="ACHDLD_002343"
     CDS             2628..2867
                     /locus_tag="ACHDLD_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364021.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2883..4256
                     /locus_tag="ACHDLD_002344"
     CDS             2883..4256
                     /locus_tag="ACHDLD_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3364022.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4260..5894
                     /locus_tag="ACHDLD_002345"
     CDS             4260..5894
                     /locus_tag="ACHDLD_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3364023.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6057..>6255
                     /locus_tag="ACHDLD_002346"
     CDS             6057..>6255
                     /locus_tag="ACHDLD_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364024.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2182 a         1024 c         1273 g         1776 t
ORIGIN      
        1 aaagcattct gtgagagaca atgatattga aaaaattcgg tttgtattta ttgatattga
       61 ccctaaaagg aaagagggaa gtgcaacaga tagcgaaaag aaaaaggcag aaaatgttat
      121 ggaacaagta gaacgttatt tgaaagaaaa gagaatagaa tcatttgtaa aagttgatag
      181 tgggaatggc taccatattt ttcttcccat aaatgaacaa ccaaataatc acgagacgat
      241 tcttactatt cagaattttc ttcaattact tcaccgaaga tttggagtag aagatgaagt
      301 tgatattgat acttctgttt ataatccttc aagattatgc aaattagttg gtacaccagc
      361 ggtcaaagga aagtccacaa aagagcgtcc gcatcgttta tctagtttcc tttctattcc
      421 cgataacata gatagaagta attgttttaa aattcttgaa cagatagttg aagaaaattc
      481 catgatgtca acctctagag taaaaagcca tccatctttt agagaagtgt taccttttat
      541 tcaagcagac tataaacaat ggttagctca ctatcctgaa ttaacttata ccataaaaga
      601 gggcgatcat aaaggagtta ccttacttat atttgacgaa tgtccattaa gaaaacacac
      661 gaataattct aatggttctt gtttgtctgt tacggctgat aataagattc gatttcattg
      721 tctgcatgct tcggataaaa ataaagatat tcaagacttt gtaaaaaaat atccgttgcc
      781 aaaacctgta aatccttctg tagaatcatt agaatccaat caagaatatt attttgacca
      841 gtttaaactg aatcaagaag gagtattttt ggtgaaagaa ggtcaagaag aaaaagttgc
      901 ttgtccgatg tttattaagc aaatacgcaa aaatatcgaa caaaaagtca ttacacttgt
      961 tcttgcctat tatgtcgatc attgttggga tgaaatagaa atagaaggtg gatgtttaca
     1021 aactaataat tttaaacaat tagcaaaaca tggcttaatc attaaagcta gaagagaagc
     1081 tgaagtcgtt gactttttac agattcaacg ggagaatgct ccaatctttt ttgaacatga
     1141 atctcttggc tggaaaaaag aaaatgaagc aatgattttt cagttacaag attcttttgg
     1201 acgtgaaatt ccttctgtat taactaaaaa ctcatgctac caattaacaa ctaatggtac
     1261 agaagcggaa tttgacgcat tggttcataa gtatgtttta ggaacgaact tagaacttgc
     1321 ttggtcgatt ggtattgctt ctattgtgtt aggctattta aacgttacga aggctaatac
     1381 ttttttgagt ttactaatta acctacaggg caagagcaca acgggtaaaa ccactacgtt
     1441 acactttatt gcctctttat ttggaaatcc aactacggtt cttagaaata tgaatgctac
     1501 caataaagcc attataaagc tagcatcaaa caatcaggga ggtgttccac taatactaga
     1561 tgagttagga gcagcaactc aaaataactt tagctcgttg atctatcaat tagcttcagg
     1621 agaagaaagg ctaagattag attctaattg tcagcttatg ccacaagaga ctttttctgt
     1681 tttgaccctt acctcttctg aagagcgttt ggaaaattat ttcaatgaga aattaactgg
     1741 tttgaaagtt cgatatatcg aattttctga tatcaagttt acaaaggatg cgaatcacgc
     1801 agagaaagtt aaaagtcttt cttctaatac ttttggttgg ggtataaagt tatttctttt
     1861 gaaactcttt gaacaaggga ttacaaaaat tgaagcaact tttgtcgaag ctcaaaaaaa
     1921 actatgggct gttttacctg aacattcttt gaagcaacgc attggtaata atctagcttt
     1981 gatttatagt ggagccctcc taattcaaga tatactgggc tatcctttac gattagagca
     2041 tatacagacg gtgcttatta attcgtacga acagttaatt gtagatacta gtttaggaag
     2101 tgagcaaacg gtatttgacc gagtgaaaac attgttggtg atgaatagcc ataaattttt
     2161 aacaaacagt gacaacaaac atacgcccag tacgatttgg ggaaaagtat caattcaaaa
     2221 aaatggggat attaaggtaa atatttttcc tatcccgttt gaggacttgt taaggcgaga
     2281 atttcaagta aaggatctaa cctatctttt taggcaattg cttgattcgg gtaatatgtg
     2341 tgcagaaaaa gggagaagaa cgaaacgtat tcacatgaat cgtcaagttc ttccaacata
     2401 cgagattcta ttaccttctt cactcaaatc gtattttcga ttgtaattta gaataaacca
     2461 aagtatgaaa gtgaggcacc atgttatgga aatatttctt attgatttag caaaacaact
     2521 gattacttat ctgtatgata gcgagcaaac ccaaacggaa gtaaatgaag cttgatctat
     2581 gcttcattta cttcattcaa tcaaacatga aagaaaggaa aattctaatg actaaaaata
     2641 aaatgacact aaaagcagag gttcttctgt atattcaaga acatttttca aatcaagcct
     2701 tttttacaaa accaatttac ttggatttcg aaataagagg ggtatctgct ggatctatcg
     2761 ggggtactct tcaagcgttg aagaacgaag gatatttaga aaatcacttt gtgcaacgtt
     2821 cgttcaatag aagagtcgtg aaaaaatggt atttagttca ttcatagaga ggggaatcaa
     2881 gcatgcgtgt ggcttgttat gatcgaacgt ctgtttatga agaagcgaaa attagtaatg
     2941 acgctcaata tgatgcagtg aaagagtatg ttttagagaa atatgaacaa attccccaac
     3001 gattttcaga caaaggagaa tcaggggaaa aagcggagcg tccagctttt cgacagctta
     3061 gagcttgggt ttgtgcgtca gaagaagagg gggtgatcta tgtttggcgt tatgatcgat
     3121 tggcacgtca agccaaaata gccttagaat ttattgagtt gtgccaacaa catcaggttc
     3181 aagtgtattc gatttctgaa cctcttcctg cttctttctc acacgcgtca acgaataaac
     3241 tatttgtgca attattgttc acttttgcag aacaacaacg aaacgtcatt attgagaatg
     3301 ttcgtgccgg actttcctat aaaaagcaac aacggcaata tttgtcttct gcggtaccat
     3361 atgggtatcg tttagtgaat gggaaaatcc aacaagaagt acacgaagca aggattgttc
     3421 gctatatttt tcaactttat ttgaccaaaa aatatggcta caaaaagctt tgtcaacgat
     3481 tgacgcaaca aaagcttttc ttccgcgaac gtccttttca gccctatcat atttatagta
     3541 tcctaaaaaa tccattgtac tatggcgaaa tcaaaggagg atctttgggg aagtatctag
     3601 gaacctttga accgattctc tcgaaagcaa cctttttaca agcccaagag attcgccaat
     3661 caaggtgtac agctaaaaaa gatacgtatc tgtacttatt acgtcaaaaa attcggtgtc
     3721 ccttctgtgg tcgtcatctg agttcgaagt atcaatggaa tacaaagaag acgaagacgc
     3781 ttcattatta tcattgtacc gaccgaagct gccaaggaat ttttcttcgg gcagaaaggg
     3841 ttgagtcaga agtattggaa aagatacagg ctttttttca atacgaaggc gtctatcaag
     3901 ccttgattgc ccaattaacg caacaattag ccgaaaagcg aaaagatgaa aaacgcaaag
     3961 taatccaaca aaagaaacaa gaagcacaat ggattaaggc atttgaagag ggcaattgtt
     4021 cactggaaga actcaagcaa caactgcaag ctcttcaaaa gccgatgtct acttcttcgt
     4081 ttctttcact ccaacagtgt gaagcaaaag tgaaagaatg gcttcaacta aaagacctct
     4141 ctagtcgaca attattatgt gactatgttt gtcggattga ggtaacaaag gataaaacag
     4201 tttgtgggat ttacttccga caactgacac aaaatatttt agaaaggaac gaatgatcta
     4261 tggaaatgat acaaaccaaa cgagtcgcat tgtatattcg cgtgagtaca caagaacaag
     4321 ctttagaagg acatagtatt caagcacaag aacaaaatgg acgacagttt gccgaacgga
     4381 tgggctatga agtggtagaa gtttatgccg atgaaggaat cagtgggaaa tccacaaaaa
     4441 atcggttcgc ctatcaacga atgatgaacg atgctcggaa ccatcgtttt gatctactag
     4501 tgatttggaa attgacccgt ctcggacgca atatgttaga tgtcttacaa acagtcgagg
     4561 aattattgcg atatgacgta ggattacatt cgatctcgga acaatttgac gtgaccactt
     4621 cgacaggtaa attgatgtta caactccttg ggagtttcgg tgaatttgaa cgcaaccaaa
     4681 ttgcggagaa tgttcaaatg tcaatgcaaa gcttagtgaa agataaaaag cgttatgcag
     4741 gaggccgacg tttaggctat gtcagtggta ttgacacgaa tggtcaaaaa caattggtca
     4801 ttgaaccaga agaggcgaaa attgttcgtc tgatctatgc aaaatattta ggcggagcag
     4861 ggtatcgagc gattgccaat tttttgaatc gtcaaggata tcaaacagtc aaaaagaatg
     4921 ctttttctac cacggcagtc aaagatatct tacgcaataa aatttatgca gggtatttag
     4981 aatatgcgcg ctacatcaat tgggaaacca aacgacggaa ggggaagaat ccgagaccga
     5041 ttttagtgaa aggaacccat gaaccgatta tttcggaaga agattatcaa aaagtacaag
     5101 aacgccttgc tttagaagga aaacaaccca aatggaacca tacaggtgaa aatgtattga
     5161 caggcttgtt acgttgtcca gaatgtggcg caccgatggc agcgagtaat gtcacaaata
     5221 ccctaaagga tggcacaaag aaacggattc gatattattc ttgttcagtc tttcggaata
     5281 aaggagcaag tgtgtgtcat gccaattcga ttcgtgccga acaagcggaa acatttgtcg
     5341 ccgaccgatt aaaggagatc gttcagctac cagaagttct tccccgattg gtggcagcgt
     5401 taaatgaaga aatagtgcga caaagccaac cattggaaca agaactcgtg gtgttactgg
     5461 aacgaaagga agaattgaag actaaaattg aaaaatggga agctgctttg gaagatagtc
     5521 ctgaattgtt tccgatgtta aaagaccgct tagacgaatt gactgaaaaa cgacgacagt
     5581 tgcacattcg agaaaatgaa atcttaggga tctttcaaca aggagagccg attcaagtca
     5641 aagatgtcca acggatattg actaatttgg atcgttttct tgcccagagt gagaagaagc
     5701 aaatcaaagc cttgtatcgt acctttatag agaaaataac ctttgatcct aaccataagg
     5761 agatgttgga aattacgatg aaattcacgc atgccgtggt acaacaattg aatgagcaat
     5821 atcaaatagc cgtatccaaa acaaaggata cggctatttt tgtgttaaaa acacccttta
     5881 cgttaacgat ctagtctagg ttatatcaaa caacgttaga ggggcgctaa cgcttctcta
     5941 gcgatttgaa atcgttagag aagtagttag ggaggtcctt atcataaata gggatattta
     6001 ttaatgaata tttgaaatat aattgtatat aagaattaaa aagagtgggg atagatatga
     6061 aaaagatggg taagtgtatt ttatgtgaaa aatacactga acttactaaa gaacatgtac
     6121 cacctaaaaa atcagggaat actggaggta aaaagaccag aactggtaat ttagatgatt
     6181 tccttaagag cgattttaca aaaggtgatt ttccgaaggg gattaaaagg aaacctcaag
     6241 gaaatgttta ttata
//
LOCUS       DAZAOG010000145         6239 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000145 DAZAOG010000000
VERSION     DAZAOG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.145"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..263)
                     /gene="rpmB"
                     /locus_tag="ACHDLD_002347"
     CDS             complement(75..263)
                     /gene="rpmB"
                     /locus_tag="ACHDLD_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN3364025.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            533..895
                     /locus_tag="ACHDLD_002348"
     CDS             533..895
                     /locus_tag="ACHDLD_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3364026.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            947..2629
                     /locus_tag="ACHDLD_002349"
     CDS             947..2629
                     /locus_tag="ACHDLD_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN3364027.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2746..4782
                     /gene="recG"
                     /locus_tag="ACHDLD_002350"
     CDS             2746..4782
                     /gene="recG"
                     /locus_tag="ACHDLD_002350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN3364028.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4831..5832
                     /gene="plsX"
                     /locus_tag="ACHDLD_002351"
     CDS             4831..5832
                     /gene="plsX"
                     /locus_tag="ACHDLD_002351"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN3364029.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5954..6196
                     /gene="acpP"
                     /locus_tag="ACHDLD_002352"
     CDS             5954..6196
                     /gene="acpP"
                     /locus_tag="ACHDLD_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN3364030.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2081 a         1088 c         1424 g         1646 t
ORIGIN      
        1 caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc
       61 caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc
      121 caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca
      181 gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct
      241 gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga
      301 ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt
      361 catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa
      421 ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata
      481 aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt
      541 aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt
      601 tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa
      661 agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca
      721 agaagataat ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc
      781 tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt
      841 aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg
      901 agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga
      961 aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa
     1021 tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa
     1081 tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt
     1141 aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg
     1201 cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt
     1261 aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt
     1321 aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga
     1381 aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc
     1441 aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt
     1501 agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg
     1561 agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa
     1621 tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta
     1681 ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta
     1741 cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga
     1801 cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca
     1861 aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat
     1921 tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta
     1981 tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc
     2041 taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc
     2101 gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat
     2161 ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac
     2221 gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa
     2281 caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat
     2341 cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga
     2401 tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca
     2461 aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga
     2521 acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat
     2581 ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca
     2641 gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga
     2701 tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat
     2761 gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg
     2821 ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag
     2881 gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc
     2941 tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg
     3001 caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc
     3061 attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac
     3121 gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac
     3181 aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc
     3241 acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga
     3301 gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa
     3361 cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa
     3421 gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa
     3481 tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc
     3541 tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc
     3601 ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt
     3661 gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg
     3721 tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga
     3781 cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca
     3841 ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac
     3901 cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta
     3961 tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat
     4021 gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga
     4081 acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa
     4141 atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg
     4201 gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat
     4261 ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg
     4321 cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc
     4381 atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt
     4441 gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta
     4501 ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa
     4561 gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa
     4621 tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct
     4681 gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa
     4741 agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt
     4801 atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga
     4861 gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa
     4921 gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa
     4981 aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca
     5041 attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa
     5101 gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc
     5161 gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg
     5221 aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa
     5281 catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga
     5341 cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg
     5401 aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa
     5461 gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac
     5521 gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct
     5581 attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt
     5641 tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa
     5701 gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc
     5761 caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa
     5821 agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat
     5881 tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt
     5941 ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa
     6001 tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa
     6061 agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga
     6121 aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag
     6181 cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagctt
//
LOCUS       DAZAOG010000146         6221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000146 DAZAOG010000000
VERSION     DAZAOG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.146"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHDLD_002353"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHDLD_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHDLD_002354"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHDLD_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN3364031.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHDLD_002355"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHDLD_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHDLD_002356"
     CDS             complement(2143..2373)
                     /locus_tag="ACHDLD_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN3364032.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHDLD_002357"
     CDS             complement(2603..3421)
                     /locus_tag="ACHDLD_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3364033.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHDLD_002358"
     CDS             3582..4271
                     /locus_tag="ACHDLD_002358"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN3364034.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHDLD_002359"
     CDS             4285..5787
                     /locus_tag="ACHDLD_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3364035.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..>6221
                     /locus_tag="ACHDLD_002360"
     CDS             5800..>6221
                     /locus_tag="ACHDLD_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3364036.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDL"
BASE COUNT         2008 a          984 c         1133 g         2096 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa t
//
LOCUS       DAZAOG010000147         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000147 DAZAOG010000000
VERSION     DAZAOG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.147"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHDLD_002361"
     CDS             725..811
                     /locus_tag="ACHDLD_002361"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3364037.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHDLD_002362"
     CDS             complement(932..1621)
                     /locus_tag="ACHDLD_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364038.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHDLD_002363"
     CDS             complement(1866..2780)
                     /locus_tag="ACHDLD_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3364039.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHDLD_002364"
     CDS             complement(2762..3142)
                     /locus_tag="ACHDLD_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN3364040.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHDLD_002365"
     CDS             complement(3592..3879)
                     /locus_tag="ACHDLD_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3364041.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHDLD_002366"
     CDS             complement(3899..4123)
                     /locus_tag="ACHDLD_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN3364042.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHDLD_002367"
     CDS             complement(4578..4748)
                     /locus_tag="ACHDLD_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364043.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHDLD_002368"
     CDS             complement(4763..5296)
                     /locus_tag="ACHDLD_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3364044.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHDLD_002369"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHDLD_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN3364045.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZAOG010000148         6005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000148 DAZAOG010000000
VERSION     DAZAOG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.148"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..919)
                     /locus_tag="ACHDLD_002370"
     CDS             complement(<1..919)
                     /locus_tag="ACHDLD_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3364046.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNK"
     gene            complement(921..2360)
                     /locus_tag="ACHDLD_002371"
     CDS             complement(921..2360)
                     /locus_tag="ACHDLD_002371"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN3364047.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2545..4491
                     /locus_tag="ACHDLD_002372"
     CDS             2545..4491
                     /locus_tag="ACHDLD_002372"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN3364048.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4755..5627
                     /gene="scrK"
                     /locus_tag="ACHDLD_002373"
     CDS             4755..5627
                     /gene="scrK"
                     /locus_tag="ACHDLD_002373"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3364049.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1903 a         1045 c         1066 g         1991 t
ORIGIN      
        1 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
       61 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg
      121 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      181 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      241 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      301 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      361 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      421 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      481 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      541 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      601 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      661 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      721 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      781 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
      841 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
      901 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
      961 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1021 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1081 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1141 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1201 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1261 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1321 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1381 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1441 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1501 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1561 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1621 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1681 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1741 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1801 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     1861 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     1921 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     1981 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2041 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2101 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2161 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2221 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2281 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2341 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2401 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2461 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2521 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2581 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2641 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2701 acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2761 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     2821 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     2881 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     2941 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3001 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3061 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3121 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3181 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3241 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3301 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3361 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3421 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3481 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3541 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3601 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3661 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3721 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3781 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     3841 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     3901 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     3961 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4021 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4081 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4141 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4201 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4261 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4321 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4381 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4441 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4501 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4561 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4621 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4681 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4741 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4801 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     4861 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     4921 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     4981 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5041 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5101 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5161 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5221 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5281 actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac
     5341 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5401 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5461 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5521 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5581 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5641 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5701 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     5761 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     5821 tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat gttacactca
     5881 aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta tacccatctt
     5941 acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc ggttgctatc
     6001 gttgc
//
LOCUS       DAZAOG010000149         6001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000149 DAZAOG010000000
VERSION     DAZAOG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.149"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHDLD_002374"
     CDS             <1..395
                     /locus_tag="ACHDLD_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN3364050.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHDLD_002375"
     CDS             398..1228
                     /locus_tag="ACHDLD_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFN3364051.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHDLD_002376"
     CDS             1244..2095
                     /locus_tag="ACHDLD_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3364052.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHDLD_002377"
     CDS             2092..2451
                     /locus_tag="ACHDLD_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN3364053.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHDLD_002378"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHDLD_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHDLD_002379"
     CDS             2624..2929
                     /locus_tag="ACHDLD_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364054.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHDLD_002380"
     CDS             2929..3240
                     /locus_tag="ACHDLD_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN3364055.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHDLD_002381"
     CDS             3237..3410
                     /locus_tag="ACHDLD_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364056.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHDLD_002382"
     CDS             3407..3823
                     /locus_tag="ACHDLD_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN3364057.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHDLD_002383"
     CDS             3823..4491
                     /locus_tag="ACHDLD_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFN3364058.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHDLD_002384"
     CDS             4488..4865
                     /locus_tag="ACHDLD_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364059.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHDLD_002385"
     CDS             4862..5161
                     /locus_tag="ACHDLD_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364060.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHDLD_002386"
     CDS             5151..5309
                     /locus_tag="ACHDLD_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364061.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHDLD_002387"
     CDS             5403..5819
                     /locus_tag="ACHDLD_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN3364062.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DAZAOG010000150         5854 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000150 DAZAOG010000000
VERSION     DAZAOG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.150"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..3335
                     /locus_tag="ACHDLD_002388"
     CDS             57..3335
                     /locus_tag="ACHDLD_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN3364063.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3876..5783)
                     /locus_tag="ACHDLD_002389"
     CDS             complement(3876..5783)
                     /locus_tag="ACHDLD_002389"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN3364064.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         1846 a         1116 c         1216 g         1676 t
ORIGIN      
        1 taaattaggt aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt
       61 taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg
      121 ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg
      181 cacacgtgaa cattgaaggg atgtcaacaa tggaggaagt accaagaact agtgaagata
      241 tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa
      301 agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg
      361 ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg
      421 gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca
      481 aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg
      541 cccaagaagg atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc
      601 aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact
      661 cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg
      721 ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga
      781 gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag
      841 gttcttataa tatattcgga aacggtcatg ggaatttgtt tccggccacg aactcgatta
      901 ctaaactgga cgtagagatg aaaaaaacga atactggttt agagtcatat tattacgatc
      961 ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt
     1021 cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag
     1081 aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta
     1141 ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc
     1201 atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag
     1261 gagaggtgct taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc
     1321 agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact
     1381 atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag
     1441 tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag
     1501 gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg
     1561 ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa
     1621 aaggcgttaa cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa
     1681 acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact
     1741 actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga
     1801 tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa
     1861 tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca
     1921 agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg
     1981 ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag
     2041 atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca
     2101 aaaactcagt tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta
     2161 atggattcaa aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt
     2221 ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata
     2281 gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa
     2341 acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag
     2401 agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt
     2461 ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga
     2521 tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg
     2581 gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg
     2641 aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt
     2701 cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac
     2761 aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata
     2821 tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc
     2881 ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa
     2941 gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc
     3001 tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat
     3061 ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg
     3121 acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac
     3181 cttctcatcc ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa
     3241 aaacgggaga atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg
     3301 tagttgttta tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta
     3361 aaacaattga ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc
     3421 cttctatctt tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact
     3481 tgatgcagga cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc
     3541 ggtattagct caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata
     3601 ctcaggtcag gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct
     3661 cggtagtaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac
     3721 tacttgaaac taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc
     3781 ttcgaaaaat aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct
     3841 tatttcttga agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct
     3901 ggcgctagat acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg
     3961 ttttttggtt gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa
     4021 agttgttggt attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat
     4081 tcctcgatag aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct
     4141 gcatcagcaa tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc
     4201 agaccatcga attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct
     4261 tctgtccacc agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat
     4321 gcatgggaaa tctcatgagc gataacagct ccgattccac cataattagc agagcttgat
     4381 tgttctagac tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga
     4441 aatggatgat aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact
     4501 ggtttgttcc atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt
     4561 gaaagcaaag taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg
     4621 atcttatcgg ggtagcctac ttggatacct aagttattta attttatgac ggctttttct
     4681 tttgttgcat ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt
     4741 tttttcacca tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag
     4801 taatcgccaa ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga
     4861 ggcattgctt cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat
     4921 tcttctgata gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat
     4981 aacggaaaat gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt
     5041 acgatcactt tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt
     5101 ttatctgtgt atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct
     5161 gcgttttctt ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt
     5221 tccgtgattg cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc
     5281 atatcaaaga aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc
     5341 ttatctggaa ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc
     5401 atatctgcat ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga
     5461 aattgcttat tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga
     5521 agcggttgag catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca
     5581 aggaaatgtt tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt
     5641 tctcccatca aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt
     5701 ttatctgcag ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct
     5761 tgtttcaatc gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta
     5821 tcttacaaaa tgaaggaatt aacaaataga acat
//
LOCUS       DAZAOG010000151         5810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000151 DAZAOG010000000
VERSION     DAZAOG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.152"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..494)
                     /locus_tag="ACHDLD_002390"
     CDS             complement(36..494)
                     /locus_tag="ACHDLD_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3364065.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(507..1838)
                     /locus_tag="ACHDLD_002391"
     CDS             complement(507..1838)
                     /locus_tag="ACHDLD_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN3364066.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1849..3291)
                     /locus_tag="ACHDLD_002392"
     CDS             complement(1849..3291)
                     /locus_tag="ACHDLD_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3364067.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3278..4042)
                     /locus_tag="ACHDLD_002393"
     CDS             complement(3278..4042)
                     /locus_tag="ACHDLD_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN3364068.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4146..5021)
                     /locus_tag="ACHDLD_002394"
     CDS             complement(4146..5021)
                     /locus_tag="ACHDLD_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3364069.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5276..5360)
                     /locus_tag="ACHDLD_002395"
     tRNA            complement(5276..5360)
                     /locus_tag="ACHDLD_002395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5324..5326),aa:Leu,seq:caa)
     gene            complement(5378..5449)
                     /locus_tag="ACHDLD_002396"
     tRNA            complement(5378..5449)
                     /locus_tag="ACHDLD_002396"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5415..5417),aa:Gln,seq:ttg)
     gene            complement(5459..5531)
                     /locus_tag="ACHDLD_002397"
     tRNA            complement(5459..5531)
                     /locus_tag="ACHDLD_002397"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5496..5498),aa:His,seq:gtg)
     gene            complement(5593..5677)
                     /locus_tag="ACHDLD_002398"
     tRNA            complement(5593..5677)
                     /locus_tag="ACHDLD_002398"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5641..5643),aa:Leu,seq:caa)
     gene            complement(5698..5768)
                     /locus_tag="ACHDLD_002399"
     tRNA            complement(5698..5768)
                     /locus_tag="ACHDLD_002399"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5734..5736),aa:Cys,seq:gca)
BASE COUNT         1760 a         1243 c          891 g         1916 t
ORIGIN      
        1 taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca actatcttta
       61 atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg agaaactaca
      121 atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat agctaacagt
      181 gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact tccttcgagt
      241 tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact ttcgatcgtt
      301 atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc atggatatat
      361 ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa gacctcgtct
      421 tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat gtttcttgtt
      481 ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt tttgtttact
      541 ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt ttgattcaat
      601 gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac tagtaacttc
      661 tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg ttttcttagt
      721 gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac tgcatctgac
      781 cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg acgatcatca
      841 ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa attaaagttc
      901 aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt atttttctct
      961 cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc aacgaccata
     1021 tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa atttttcgtg
     1081 atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt aaaatcggcg
     1141 acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc tcgttgcaat
     1201 tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata cgtaacgatc
     1261 caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga aatagtcata
     1321 tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc agaaacgatc
     1381 gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg tatatgatcg
     1441 acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc gagtccacac
     1501 gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc tcgcatctcc
     1561 aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga
     1621 tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat aaatttaaca
     1681 atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat ttcagataat
     1741 ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata cgtactcccg
     1801 cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct attcttctaa
     1861 taatttattg aattctgcca taacataagt cacttctaag ccgatgataa tttgataatt
     1921 tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct gttcctcgac
     1981 catggtggga tccactacct ctacccgtag gcgtgtatta caactataga catccaggat
     2041 attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat aatttttact
     2101 tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg ccttagcttc
     2161 acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa
     2221 ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga ttcgaaaatt
     2281 gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat tgaataagta
     2341 acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca taggagaaag
     2401 aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag ttcctgcacc
     2461 gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt tatagatagc
     2521 tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata aaaacagtcc
     2581 agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt ccttgctagg
     2641 agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt gcagtccaaa
     2701 ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc ccatatcaac
     2761 gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa agcccaaaaa
     2821 aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt cgcccgcaaa
     2881 ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc atgccgctac
     2941 taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca taatttctaa
     3001 gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt
     3061 atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt ttccaaaaac
     3121 ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg ataaacgcat
     3181 gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc caagcttctg
     3241 cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca tatgttacta
     3301 actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga
     3361 cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga tccaaatacg
     3421 ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt aaacttgcca
     3481 aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct cctacgacat
     3541 cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca aagttgctcc
     3601 gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt tcatccaaag
     3661 tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga taaatttttt
     3721 gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat ggatgctgat
     3781 aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt ttgaggagtg
     3841 gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt tcctttctta
     3901 gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat gtcaaaattc
     3961 ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca aaatcatccg
     4021 aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa taggcgcaac
     4081 caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat agttacgccc
     4141 ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt ttcttgaatg
     4201 gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca atacgctttg
     4261 ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc ttcctgataa
     4321 atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt cagtgattct
     4381 ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt cgtcatttct
     4441 actattttta agctctttaa actattaggc tctcctagct cagcattaat gatatgcata
     4501 gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt gattcgctcg
     4561 accacttcta ctgctatatc atattcattt ggataaagga atctcatttc tggcaacata
     4621 attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat atgatctgtc
     4681 aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat cagttcaatc
     4741 acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc actgaatcgt
     4801 ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac atcaaatggc
     4861 ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc tattccatcg
     4921 tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt taattcacct
     4981 cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt tgtctccaaa
     5041 caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg tgcctaattg
     5101 aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa gcgttatcaa
     5161 tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag gtaattacat
     5221 aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat aattataccg
     5281 gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct
     5341 gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg attcgaacca
     5401 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaggg
     5461 cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc
     5521 gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact aagatgaaga
     5581 catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg gcactggatt
     5641 ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt agtactaagg
     5701 cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta ccacttggct
     5761 ataccgccat tatactatta tcaatctata aaataaagat taaattatag
//
LOCUS       DAZAOG010000152         5809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000152 DAZAOG010000000
VERSION     DAZAOG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.153"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACHDLD_002400"
     CDS             450..1604
                     /locus_tag="ACHDLD_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFN3364070.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACHDLD_002401"
     CDS             1577..2020
                     /locus_tag="ACHDLD_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364071.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACHDLD_002402"
     CDS             3143..4075
                     /locus_tag="ACHDLD_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3364072.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACHDLD_002403"
     CDS             4092..4607
                     /locus_tag="ACHDLD_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3364073.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACHDLD_002404"
     CDS             4903..5184
                     /locus_tag="ACHDLD_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364074.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACHDLD_002405"
     CDS             5186..5602
                     /locus_tag="ACHDLD_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364075.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZAOG010000153         5806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000153 DAZAOG010000000
VERSION     DAZAOG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.154"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1027
                     /locus_tag="ACHDLD_002406"
     CDS             131..1027
                     /locus_tag="ACHDLD_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3364076.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1177..2118)
                     /locus_tag="ACHDLD_002407"
     CDS             complement(1177..2118)
                     /locus_tag="ACHDLD_002407"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3364077.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2244..3365
                     /locus_tag="ACHDLD_002408"
     CDS             2244..3365
                     /locus_tag="ACHDLD_002408"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3364078.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3623..5125
                     /gene="eat(A)"
                     /locus_tag="ACHDLD_002409"
     CDS             3623..5125
                     /gene="eat(A)"
                     /locus_tag="ACHDLD_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3364079.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5192..5719
                     /locus_tag="ACHDLD_002410"
     CDS             5192..5719
                     /locus_tag="ACHDLD_002410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3364080.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1896 a         1027 c         1221 g         1662 t
ORIGIN      
        1 atgatttatt tcatcatatt ttcatacaaa tgttaaattt tcatacaaat ataggaaacg
       61 ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca gagaaagtga
      121 ggcggaaaag atgaacaaaa atgacgtact gctagttgca aaaaataaat cactttattt
      181 tttgccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac agtcggaatt
      241 cattaaatca gctagacaac agttggcaaa ttatcagctg atcgtttttt tagattatgg
      301 atttgatatg gagatggctc gacgcgtgcg cccatttacc aatgcaaaaa ttgtgttatt
      361 tttttggaat cacttcaaag cagaacacga ggcgatgctg aagcaggcac aagatgaacc
      421 ggcaatcgat gagatttatc attttgattt tttggaagca aaagaacttg ggctgaaaca
      481 taatagttca ttttattcga aacatatgaa tttttcttct agcgaaccaa agacggatct
      541 attttttggt gcaacgaata atgggagaaa agaacgagca gaaagctata agcaggaatt
      601 tataaaaaga gatgttacga ccaactattt cattcttcca catcgaggga atgaacaacc
      661 tggatatctt acttatgaag agtacctgaa aaaaacaggg gaatcgcgcg ggattctgga
      721 actgttgcgg gagggtcagc aaggagtgac tttgcggaca tttgaatcca tgtattttca
      781 aaagaaacta gtgacggata atcaagcgat tgctcattat catttttatc gtcctgaaaa
      841 tatttttttg cttcgagaac gtgatttaga tgaattaccg gaatttctgc atacaccatt
      901 taagccaatc ctatcagagc tattggaatt ttatgatgca gaaaattggg caaaacgatt
      961 tatcactaac gattccacta tttatgagca atacgaatat gacctctcct ctgttgaaaa
     1021 caaataaaaa acctacattt tgaagagatt atgtactgaa acaaacaagc acaattctta
     1081 tggcatcatg agagttgtgt tttctttttg atcttttatt gggcttaaaa aaagagaacc
     1141 agctgctgat tcagctgatt ctcatcagag agccttttat tctgcgccaa ggattccttc
     1201 ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat catatttttg
     1261 tccttttcgc caaatggcac cattgatgta gtcgatttca gtcaaacgat ggtttttgat
     1321 caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc
     1381 aatatgttgg tacacttctt cttgatctag tatgattcct tcttttgctg cgatagaagc
     1441 aaattcagat acgattgttc gaaccatgct ctctgccgct ttttgcgcgc ctagttcagc
     1501 aatattacaa tccaaaatcg tgcataaacc attcaatgtt ccatttacac aagcttttcg
     1561 ccaaatggaa taacgaacgt tatggctata gatcggattt aaatttgctt cttgaaagac
     1621 ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta gttcgatttc
     1681 tccatcacct aacaatgtga cttttccggg accagctaga ccagctgtcc acatcgtgat
     1741 cccaaataag atatttttct ttggcacata tttttccagt acatcttcat gacctaatcc
     1801 atttagtaaa cacaaaacat aagtattgtc agtaataatg gattggatcg aacaaaacat
     1861 atcatctaat tgattggctt ttgttaatgc tacgatcaag tccacgtgtt cgttgctttc
     1921 gatgatttct tctggtgaat agattggcag ttttgcgacc acttctttac cgttaaagtc
     1981 agcaatcaac ccatttgtac gaatggcttc aatgtgagca ggccaacgat cgatcaagag
     2041 gacctcattt ccgctttgat gtagcatcaa tccgattcgg ctgcccattg ctccggcccc
     2101 agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct tttagtatat
     2161 agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa gtgtcttact
     2221 taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca tttaaaggaa
     2281 gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat cgtaaaggaa
     2341 gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca atctattacg
     2401 cgctcagggg aacatggcgt accattgatg ggatggatct cttattccgt cagcgaaaaa
     2461 tcagctattt attgacaact tggcaaggaa aaaacaccgc agtcatcgaa tctgcacaag
     2521 cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt gcttccagtt
     2581 atgccttagg agaacttatt aaagatgcac tgcttcaaga agtagaacaa attattgtca
     2641 ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc ttgggtgctt
     2701 ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt cattttgatc
     2761 gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt accaatccat
     2821 atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga atagatacga
     2881 cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa gatttctggg
     2941 aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt ggagcccttt
     3001 ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc actggattgg
     3061 aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc gatcagcaaa
     3121 cgatccacgg aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa
     3181 taatcgctat ttgcggaagt agagaccaag agctagaagg actttctgat tttctcccaa
     3241 ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa cacaggcaaa
     3301 cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc atcctcccga
     3361 agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg acaaagtgag
     3421 ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt
     3481 ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg atgtatgggt
     3541 ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat aggactggtc
     3601 tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga cattcggcta
     3661 cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca cacaatggaa
     3721 actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata ttctacaaaa
     3781 caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca
     3841 gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg attttgagat
     3901 atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct tatggagaga
     3961 attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt tatttgtgga
     4021 tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggtagaaa
     4081 acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg
     4141 gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc aactggaact
     4201 ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat
     4261 ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa
     4321 agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg gattcatcga
     4381 tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac ggacgatgaa
     4441 acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag aaaaactatt
     4501 aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc accataagcg
     4561 acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg agccaattca
     4621 ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg caggaaagtc
     4681 atccattatc cattatcttc tgggggcatt caacggcaag gttataggag aaaaatacca
     4741 gccaaaacat ctgagcatta gttatgcaag ccaaaattat gaagacaatc gaggaacgtt
     4801 ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca acctccgaaa
     4861 gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta tgggccaacg
     4921 gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata catgggatga
     4981 accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga tcttgaacgt
     5041 gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag tatctactga
     5101 gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag gtatggttaa
     5161 ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac acggagaaac
     5221 aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg
     5281 acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa
     5341 aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg ctgatcgtct
     5401 cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg attatgatgg
     5461 actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg tacgattccc
     5521 aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga aagaatgcat
     5581 cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa gagtgatcaa
     5641 tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag ataatacaga
     5701 attggtttct tatgagtaat tatgtttttg tgttatttaa ataaaatatt tcaaggcact
     5761 gcttttgctt agcagtgcct ttttttaggt tatgacaatt cttttg
//
LOCUS       DAZAOG010000154         5692 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000154 DAZAOG010000000
VERSION     DAZAOG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.155"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..695
                     /locus_tag="ACHDLD_002411"
     CDS             3..695
                     /locus_tag="ACHDLD_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364081.1"
                     /translation="MYYKKQTILYCLFSSFSAFLLIACLTLYHLQSALANQVQDRLSF
                     IGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLTGL
                     SKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDI
                     LTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIH
                     HLQSRRPGRNET"
     gene            685..1194
                     /locus_tag="ACHDLD_002412"
     CDS             685..1194
                     /locus_tag="ACHDLD_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364082.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1264..2712)
                     /locus_tag="ACHDLD_002413"
     CDS             complement(1264..2712)
                     /locus_tag="ACHDLD_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3364083.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2712..3428)
                     /locus_tag="ACHDLD_002414"
     CDS             complement(2712..3428)
                     /locus_tag="ACHDLD_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN3364084.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3409..3759)
                     /locus_tag="ACHDLD_002415"
     CDS             complement(3409..3759)
                     /locus_tag="ACHDLD_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN3364085.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3903..4676
                     /locus_tag="ACHDLD_002416"
     CDS             3903..4676
                     /locus_tag="ACHDLD_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3364086.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4847..5213
                     /gene="ssrA"
                     /locus_tag="ACHDLD_002417"
     tmRNA           4847..5213
                     /gene="ssrA"
                     /locus_tag="ACHDLD_002417"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1668 a         1112 c          985 g         1927 t
ORIGIN      
        1 taatttatta caaaaagcag acaatactct attgtttatt ttcaagcttt tctgcttttt
       61 tattgatagc atgcctgact ctttaccatt tgcaatcggc tttagccaat caagtccaag
      121 atcggctttc atttattggc gcttctgcat ctacagagct tcttccggct ttagaattag
      181 ctagccgatt ttatataaaa ggcatattca gtttattttt tctatttgcc ttccttttta
      241 tctttttctt ttactattca gtcaggcaaa acaagcagga attgatcaat tggcgcctga
      301 ctggtctttc taaaaccaaa ttattccttt ttattttctg gcagctgttc tttcctctct
      361 ttctttgttg tatcttggtt ttattgtgga tcgtaagctt ccagtcagtt tatgaagcgc
      421 ttcttcaaag gatcaatttt cttgtcttaa agactactga actgccagat atccgtgata
      481 tcttgacttc ggtaagcgga cttacaattc cgatggatca acagactttt tttaccatcg
      541 atttccaaaa tgatcttttt ttcacagata tacttaaggg atttgcccaa actcttattg
      601 ggctcagtgg atgctgtctc attcttagtc tattcccttt cacttttttt atccatcatt
      661 tgcaaagtag gaggcctgga cgtaatgaaa cctgaatatt tgattcatgc tgaacacttg
      721 accgttattt tgtgtcctct cgaaaaacag cataaaatat tggagcactt gaaaaaagag
      781 gttcttgttg caaaactgga taattttgcg atcatccaaa aaaattggcc gatgtttcct
      841 tatctgaatc ttaaagacca tgtcctgttg gatgtaccgg aaaaagaaat caaacaagat
      901 cgtttggcct atcaagaaaa acttgatatc tctccttcct tgcttaattg tgcggcagct
      961 gagttgacgt cttttgaaaa agtaaagctg caattacttc atgccttgct ttccaagcga
     1021 aacaatattg ttattgaaga tacatttgat gaacttagtg tattggagat ccaagagcta
     1081 ctccacttgc tttcttattt agctcatgaa gaaaatcaag gtatcttact ctttactcat
     1141 gatgctacta ttgctcaaag tccttatatt gatcgtttag agcccgcggg ctaaatcaat
     1201 cattttatag taaaaacaag cggtatagaa gatcgattca tgatctttta taccgcttgt
     1261 tttttactct tttttgattt ctacgtcagg caaacgaaca gcgaattttg ttccttttcc
     1321 tagttcgctt tctacttcta attttccttt atgcaagcgt acaagttgct gcacgattgg
     1381 taaacctaat cctgattcac catattttgt attttttctt gaaggatcta ctttataata
     1441 acgatcccag atacttttca tttgttgttc agacattcca attcccgtat cttcaatcgt
     1501 gatgatcgtt tcaagatagc ctttttctaa agtaatcgtg atttggccgt tttcagtaaa
     1561 ctgtatcgcg ttttgaataa tattgaccat gatttggaca aatcggtcat aatcagcgta
     1621 aacatcaata tcatcaacgg ctttcaatgt caatgtatct cctgctgctt ctgctttagc
     1681 ttctaactgt gcaacaatat ttttcaatgt ttctgttcca ttgaattttt taatgaccat
     1741 actgatttga ttcgtacgga ttttttcata atccaagttt tcattcacca aacggatcag
     1801 acgctctgtt tcatttttca tcaatttaat cgcgttttct cgctgatttt cagggattgc
     1861 attgtattct aacccttcca gaagtccgtt gattgtcgta agaggtgttc tcatctcgtg
     1921 agaagcatct gccataaatt gtcttcgacg attttcttgt tcttcaattt ctgcttgaga
     1981 ttctttcaat gaattcgtca ttttattgaa atcatctgct aactcatcaa attcatcttt
     2041 gtcatgtacg gggagctgta catcaaaatt accattcgtc acttctttcg tcgcattcct
     2101 taaccgattg atacgcttaa cttggaatgc tgcaaaagca tagctggcaa taactgcaac
     2161 gatactggaa aaaataaatc ctttgaacag gttcagtgta attggacgaa cgctgttatc
     2221 gatatttcgc gcaggttgac taactacaag tgctccatag aattcatgat tcaaattaaa
     2281 tggcacaagt gcgtaagagg tcgcctgtgc ttgtcctaaa atatttttat tcgatgtgaa
     2341 tttttcttga cgtccattcc tcagattttt ccattgatca gctgttaagg taaaattcag
     2401 taaagctgta ttagtcggat aaatgacacg ttcgttttta tcgataaata taaagttcac
     2461 attttgttct gtcagtactt gctctgtcag cagcaatgca ttttgaaacg cctgatcttg
     2521 actaaactgc gggagcgtat cggcataggt ttgcgttgtc ttttcaactg attcagcata
     2581 tccaaacagt tgctggtaat tgttttcttc aatcgtttgt tttgtcagtt gtgtaaaaga
     2641 aactccaacg atcaataaga tcaacgcgac cattccgcaa aaagctaaga gttgctgata
     2701 caaataacgc attaggcaac accagaatca tcgaatttgt agccgactcc ccaaacagtt
     2761 tggatgactt gtggtccgac cttttcgatt ttttgtcgta atttcttgat atgggcgtcc
     2821 actgtccgtt catcaccaaa gtactgataa tcccagacta attcaagcaa ctgctcccga
     2881 gagaatactt gacgtggttt ttttgctaaa gtaaacaata gatcgaactc tttaggcgtc
     2941 agtccttcaa ttggccggtt gtccagatac gcttctcgtg ttttcgtatt catcttgaaa
     3001 tgactagtct cgatatcaaa atgatcatct gaaactgctg cttcttctct tgcttctgcc
     3061 aactcactac ggcgatgcaa agctttcatt cgagcaataa gcgtcagagg actgaaaggt
     3121 tttgtgacat aatcatctgc ccccatctct aagccgatca cctgatcgct ttcagaatct
     3181 ctagctgtca gcatgataat cggcacagta cttgaaactt tgcggatctc tcttcctact
     3241 agcataccgt ccagtgtggg caaattcagg tctagtgtaa tcatatccca atgttgtggg
     3301 ttttctaaaa aggcatcgag cccttctttt ccatcatact tgaaagttgc ttcccaacct
     3361 tcatttaaga aaaacatctg catcatttca gaaacagatt gattatcttc aatcattaaa
     3421 atattcatta tcgttccctc cagtttccag gtcatatttc tgtaatttct tttctgtcat
     3481 aataaaaata ggcataattg gaaatactag cagaagcatt tcaatcagaa aataattcgt
     3541 atgcccagat gtctttaaat aatagccgat caatgccatc agtaagccca taagaaaaaa
     3601 ccagtttccg cttgtctttt gagccagccg ccaatgagcg tctgtttttg cggcaagagg
     3661 cgaatgatac ccataaaaag gcagatttcc ttttgagggc agaatccgaa aaagactgcc
     3721 aattattaag aataagatac caatactgaa aaaaatcatt ctttccctcc taaattctct
     3781 tcttatagta tactctaaat tgttgactga accaatatgt tcaggaattc ttcacgaaag
     3841 gtttgtcttt tgttcattct ttgttaagat aaacttattc caataaaaaa ggagttttat
     3901 ttgtgtcaaa ttatcgtgca atcactttct ttgatcttga tggaacatta ctagatgcga
     3961 aatcaaaaat cacacccgaa gtggcaaaag ctatgaacgc attaaaagaa aacaatgttc
     4021 tccccgtcat cgctacagga cgtacagaag cagaaatcca ctctatcatg gaagatgcgg
     4081 gcattacttc tgcggtcacc atgaatggtt ctttcattcg agtagacggc gaagaaatct
     4141 attctcaaac attttcttca gaagaatgtc aaaaaatgta tgatcatgta cagttgaacg
     4201 gtcatgaact ttcttactat aacgatcaga aaatctggtg taccggtcat agccaaacaa
     4261 tgatcaatgc ttacgacttc attcattcag atgttccgga aattgatcct ttaggcttta
     4321 aagaaaacac agtaaatatg ttgctgatct tatctcaatc aggtgacgaa tactatcacg
     4381 aacattttcc tgagctaact ttttatcgga atggtcctta ctcgatcgat acagtgaaga
     4441 aaggaatctc aaaaggggca ggtgtcaaac gtctgcaaaa agaacttcag ctggaaaaag
     4501 ttcctacttt tggatttggc gatggtccga atgattttgc cttgttggaa gcttgcgaca
     4561 acaaaatcgc tatgggcaac gcctatgacg gattaaaaga actttctacc tttatcacaa
     4621 agaaaaatac agaaggcgga attgtccacg cattgaagta ttttgatctg atttaagatc
     4681 tttttttgaa gctacctctt ttcttaagaa aatcgcttat tttttcttaa gaaaggggca
     4741 gttattatat aaataatggg atatgattcg cttttttatt gaatttatcc caatatatgc
     4801 tatactaatt ttgtatgaaa aatacacgta acccttgtta gctaacgggg acgttacgga
     4861 ttcgacaggc acagtcgagc ttgaattgcg tttcgtaggt tacgtctacg taaaaacgtt
     4921 acagttaaat ataactgcta aaaacgaaaa caactcttac gctttagctg cctaaaaaca
     4981 gttagcgtag atcctctcgg catcgcccat gtgctcgagt aagggtccta actttagtgg
     5041 gatacgtttc aactttccgt ctgtaagttg aaaaagagaa catcagacta gcgatacaga
     5101 atgcctgtca ctcggcaagc tgtaaagtga atccttaaat gagttgacta tgaacgtaga
     5161 tttttaagtg gcgatgtgtt tggacgcggg ttcgactccc gccgtctcca tatatgtatg
     5221 gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc
     5281 aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca
     5341 ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa
     5401 agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca
     5461 caattatggt ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat
     5521 taattcatcc taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca
     5581 gcaggcttca cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata
     5641 attgctttaa ttattttttg acttctcttc aaacacaaga agtagttgtg ta
//
LOCUS       DAZAOG010000155         5688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000155 DAZAOG010000000
VERSION     DAZAOG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.156"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            597..1508
                     /locus_tag="ACHDLD_002418"
     CDS             597..1508
                     /locus_tag="ACHDLD_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFN3364087.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1995..2261
                     /locus_tag="ACHDLD_002419"
     CDS             1995..2261
                     /locus_tag="ACHDLD_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364088.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2333..2674
                     /locus_tag="ACHDLD_002420"
     CDS             2333..2674
                     /locus_tag="ACHDLD_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364089.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2729..3148
                     /gene="ssb"
                     /locus_tag="ACHDLD_002421"
     CDS             2729..3148
                     /gene="ssb"
                     /locus_tag="ACHDLD_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3364090.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3225..3521
                     /locus_tag="ACHDLD_002422"
     CDS             3225..3521
                     /locus_tag="ACHDLD_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364091.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3536..3967
                     /locus_tag="ACHDLD_002423"
     CDS             3536..3967
                     /locus_tag="ACHDLD_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN3364092.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4272..4454
                     /locus_tag="ACHDLD_002424"
     CDS             4272..4454
                     /locus_tag="ACHDLD_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364093.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
     gene            4858..5346
                     /locus_tag="ACHDLD_002425"
     CDS             4858..5346
                     /locus_tag="ACHDLD_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3364094.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            5333..>5688
                     /locus_tag="ACHDLD_002426"
     CDS             5333..>5688
                     /locus_tag="ACHDLD_002426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364095.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQF"
BASE COUNT         2118 a          818 c         1149 g         1603 t
ORIGIN      
        1 tccgacttcc aggtttttga ccattttctc tcaacgtgga aaaaccgcca cttatgagag
       61 aaaatggtca aaaacctaag cgggaagtct ccgaataaca taccaagcgc cgacagccaa
      121 cagataaaga atacactact aaaaatagta gtagcataaa atttaaaagg gaaaaaatca
      181 acccctaccc cagtcttggg gtctcaaaac taactccccc ctaaccccct ctttcagcct
      241 cttttgaaag agggggagaa tcaaccccac ggccaacgcc ccagtgttct tggggctgtc
      301 cagaacagaa acttgcgaga gagtgggggt aaaacaagtg tagaggaaac ttgaagactt
      361 gttcgaacag aaacttgcga gagagccgga gtaaaacaag tgtagaggaa acttgaagac
      421 ttgttcgaac agaaacttgc gagagagttg gagtaaaaca agtgtagagg aaacttgaag
      481 acttgttcga acacaaactt acaagagagg ggagtaaagc agttgcagag gaatcaggaa
      541 gatttgttcg aacagaaact tgcaagaaag cattattaaa ttgaaggtga tgaaatatga
      601 agtatttatt gcatggctat tttattgatt attctactcg caatagtagt actttagcga
      661 ttctatgttt gcttcatgaa ttgagagtgg ttactagaaa acagctatac aaactattga
      721 attttgacag atcaattagt aaacaaggag taacgaatgc tttagtgaga ttatatgaaa
      781 aacagttaat cgatcgaaaa aaatatggaa cagaaatgta ttattatttg acgaaagagg
      841 gacatcaaag cataggtggg tattatacgt tgccgaaagt tcctgaatat aatttgaatc
      901 accatttaca aattaacaat tatttgatta aaggaatggc attggcaaaa gaccacccac
      961 atttaaaacg gattatttca gaaagacgta ccgtctatga aacgaaggat ttaaatccta
     1021 agacaaaagg aagaaaattt tttgtgccag attttatttt tcgttttcga gacaaagaaa
     1081 atcatgagat caattggtcc tttgaaattg agctaacgtt gaaaacacgt cgtcgctacc
     1141 gtgaagcgat ttttccgaaa tatattcgtg agttgcgaac acgtcctgaa tcacatttaa
     1201 tttatgtgac acctagtggc gcaattcaag aagaacttca tgcgtttaag cgtatttttt
     1261 tcaagcgcga aactgaaaaa gatgcgagtc aacaagaatt gtttgagcgg ttgcatattt
     1321 tttcagcttt agagtttgaa gagggattaa aagcattatt agagaatgat ccagcagtca
     1381 attggcggac agtagaacga gaaaaaattt atccgaatgg cgctgttcaa agtcgtttga
     1441 atttatttga agaatcaact gaaaatgagg aaaaaccaaa agtgaagttt acgcctgaag
     1501 tattgtaaaa gagggtgttc gcttcgctct ttttttgtgc cagtttgctg gcaccgttca
     1561 gacgcaagcg tctgaaccgg ctcgttttta ggaaggacta acttgtttga tttatccgtt
     1621 gtcaaagaag tggacgttcg agagtcccta atcgtatagc agtcattcgg cgagtataac
     1681 ctaaaacata acctgccaat tgccaagggt aaaatgcacg ctgcgcgccc ttggcaatcg
     1741 tcagaacatg ttttcgagcc ggttatgccg aacgaccgct atacgaaaag ggcctctaaa
     1801 cagaagtcca ctttgatcct tgacaactga aacccaaaat caaacaagca ggccttcgtc
     1861 taaaaacgag ccgaatgaag accttggggg ttgcaccccc aaacccccgt gcccccgaca
     1921 cactaccgaa aaggtggggg tgggtggcga aaaaatcaaa taaaacattc tattcaagaa
     1981 aggaagaaac aaccatgaca actaaaaacg agcaactgaa agaacaggca agaaaagagt
     2041 tgcaagaaag aggtttgatt attgagggga gctttgaagg agattttgaa acctatattg
     2101 gttgttatgc aagaccgata aacaaaccta cagccttaga cccaatgaac gaacaagaag
     2161 taaaagaaca agaaaaacat gcagtgaatg gttttccgca agatttcact gaatggtatg
     2221 agtgggaaat taacaacggg aagcttgaaa acttcttata aaagtcagat gacgtgcaaa
     2281 gagaatgatt ctctttgtac ggtaaaaata taaagaaaga aggaaaaaga aaatggcaaa
     2341 aaacacacct tatcaagtag gagacatttt ttatagtagt tggggctacg aacaaacgaa
     2401 cgtgactttt tggcaaattg tgaagttaac agaaaaaaca gcatggtttc agcctattca
     2461 aaagaaaacg gtcgagcatt attcgtccat gagtggaaaa gttgttccaa tgaaagacaa
     2521 gtttatagag tatcctttag caaaaactaa agacagtatt gtaaaaaaac gaattgatcc
     2581 agaaacctat cctgatatgt tatgtggtta tcgtagttgg gacacgtttc atagatataa
     2641 aaatcaagct gtttatgaaa gtagttatta ttaaggggat agtttcccct ttcaaaaata
     2701 ataaatagaa gaatggagag aaaaaatcat gatgaattca gtgaatttag taggacggtt
     2761 aacaaaagaa gtggatttaa aatacacacc aaaaggcaat gcgacaggca catttatttt
     2821 agctgttaat cgaaattata cgaatgcaaa cggcgagcgt gaagcagatt atatccgctg
     2881 tgtcatttgg cggaaagctg cggaaacttt agcaaaatat actggaaaag gaagtttgat
     2941 tggcattaat ggacgtcttc aaacgaggag ctatcagaat cagcaaggac agaccatgta
     3001 tgttacggaa gttctagtga cagatttcta tttgttagag agtaaagaaa tcaatgagca
     3061 acggtcaaaa tcaatggcga ataatcaaaa taattcttca attccaggaa atgagattca
     3121 aatttcagat agttatttgc ccttttaagt gaaaaataat tttgttctat acctgatttc
     3181 ttcaggtata gaacaaaaaa taaatagaga tgaggcgatt ttttatgaaa atcattgaca
     3241 gttacgatca acgaaccatg caatatttgg gctcgtttga ttatacagat gaaaatatta
     3301 ttaattatgt tgcaagttta cctccttatc aaagcgttcg cttggtagat tttgcaagtg
     3361 atgaattggt attaacaacg atgggcaact ttttagatta tgtcggagac aaaaaatggt
     3421 tggaaaaaat tttgtctaaa ttgattgcga aacaaacagg agaagaaccc ataaatgcga
     3481 ttcaattgtt caatcgatat gaacaagaaa acaatgatta aagaggagag aaaaaatgaa
     3541 aagattaaaa cgaagtattt ttattgtgct agcattgatt gattttgttg ggacaggttg
     3601 tgaagcaatt gacgttcaaa aagctccctt atcggaaata aaacaagcaa taacaaaaca
     3661 agatgaagaa caaccgctaa atacacaagt agaaatagtg aatcaaaata aaccgagttt
     3721 ttctgaagaa gatttttcgt tagaaaatgg aaccaggcaa tttttttcag atttggatcg
     3781 tttaaatcgg gtaggtgtag caagtgcact tttgcataaa agcaggatgc caacagaaga
     3841 acgagaccga ttatacatta agccaactgg ctggaaacaa aagaaaatga agaatggcga
     3901 ttatctttat aatcgttgtc atttgattgg ctatcaacgt gcgacaagtt tccccataag
     3961 tatgtaatac gaaacgggag gattaatctt aagattaatc acaactaacc tatttgacag
     4021 ttggaatgaa agctatcatg tctgctgaat caactggtct gatactccta catgcaataa
     4081 agtctactag gaagttattg tatgaagcta ggtaaaggcg gcagaacaat ttctaagtgt
     4141 ggaatcatat gaagttggat atgccgggac gctataaatt actcagggtg agactgtttt
     4201 taattaaact gacgaacttg cgaatgtacg ggtctataac cgacttatat tcgaaagata
     4261 taagattgcg aatgcaaaga acattatcga aaagtaagaa tgttggtcat gaaattcgag
     4321 atggaagact agcggtgtat ctgtcaagag ttggtaaaac atgtttgatt gattcagatg
     4381 agagtgggaa tgcgacaaaa cgttttacag aagaaataga gcaactagca gaactgaata
     4441 tacgatattt ttaaatactt tagcggagaa taaaaaaatc ctctacagaa aactacggag
     4501 ggcaacttgt gctattgttt caacaataca tttttaattt ttaccataac aaaaaagtac
     4561 ttgaaaaaaa acagaaatat gttaaattaa aaatgaaaaa atggaagcgg ttacttattc
     4621 ttttgaatct aagataaaaa ttcaaaagaa taacataacc caaatatgat tatacttgag
     4681 attctaaata tttatcgaaa aggaatttgt tttaaggaaa aatgattcta tggacggtta
     4741 ttgatttata tttaccaaat ggctaataaa atagtatttc ttatattgta tagtttttgt
     4801 ttttatgtga aaacatgagc aatagaattg tactagtagg ataaggatgg atggaaaatg
     4861 aaaaaaacta tttttgtttt agcgattttt atatgttttg ggctactttt ggtaggctac
     4921 ttgttgttta attctaatga aatgagaact atttctacga atgacttgtt aaaaaaaagt
     4981 gaattaaagg ataaaaaaaa agaatctctt gcctttataa tgtttactca agaagagtgt
     5041 gagtattgta tagcaataaa tccaatcata gatagtgtaa ataaggaaac tttgaataag
     5101 ataaagaaat atgatatatc tgtagatttt caatctaaac aaaagtgtaa aattaagttt
     5161 gttccgacta ttgttgcata tagtaagaat aaagaagtgg ggagaattga caatgaatta
     5221 gataatttgt atcgaaaaaa gcaagatggt tcaattgtaa tagacaagaa aaaaacaaag
     5281 acattgctta tagattggat gaaaaaaatg gataaacaag gagaatattt gtatggagaa
     5341 gattaaacag acatttccgt taattaataa atattggaat aagaaaaaaa tttttatatt
     5401 ttttattacg tttctgctgt catctttatt ttctctaatt ttcccgtatc tatttagtaa
     5461 tgttattgat ttatatttag agaatgagtt atcacagaat attgtgcgaa tagtaacgat
     5521 tcttatattt cagttatttt tagtcttaat taaagaagtg tatggatata taaaaactat
     5581 ttatgcattt gaagttgatt tatcaatgaa aattgatttt cttaaggttt tacaaaaaaa
     5641 aaatcttttt ttttcgcaaa cgaagcattc aggagaaatt caatttag
//
LOCUS       DAZAOG010000156         5636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000156 DAZAOG010000000
VERSION     DAZAOG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.157"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      188..354
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            502..1059
                     /locus_tag="ACHDLD_002427"
                     /pseudo
     CDS             502..1059
                     /locus_tag="ACHDLD_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="ACHDLD_002428"
     CDS             1117..3156
                     /locus_tag="ACHDLD_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN3364096.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="ACHDLD_002429"
     CDS             complement(3265..4416)
                     /locus_tag="ACHDLD_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN3364097.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="ACHDLD_002430"
     CDS             complement(4570..5100)
                     /locus_tag="ACHDLD_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN3364098.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="ACHDLD_002431"
     CDS             complement(5199..5549)
                     /locus_tag="ACHDLD_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN3364099.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2044 a         1033 c         1050 g         1509 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5581 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaa
//
LOCUS       DAZAOG010000157         5619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000157 DAZAOG010000000
VERSION     DAZAOG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.158"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..2235
                     /locus_tag="ACHDLD_002432"
     CDS             175..2235
                     /locus_tag="ACHDLD_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN3364100.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2318..2887
                     /locus_tag="ACHDLD_002433"
     CDS             2318..2887
                     /locus_tag="ACHDLD_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3364101.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3062..3868)
                     /locus_tag="ACHDLD_002434"
     CDS             complement(3062..3868)
                     /locus_tag="ACHDLD_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364102.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4006..5520
                     /locus_tag="ACHDLD_002435"
     CDS             4006..5520
                     /locus_tag="ACHDLD_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN3364103.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2004 a          969 c         1132 g         1514 t
ORIGIN      
        1 ttaacaaaat taaaaagtac ataagttatt attccgattt tcttgaaaca ataaaaaatc
       61 aaaaaaaata ctctcctaat agtccatctt cttttgattt tatttatata atgaaatcat
      121 cttatgaaaa ttactacttc atgtttagac aagagtaggg aggactaaga ataaatgtgt
      181 tcaaaaagac gttcaaaaca actaaataaa cacgcttgtc gtttagataa ggaacggtcg
      241 tgttctgaaa aacactacgt taagtttatg atgcgcacga tgctgatcag tttatctgta
      301 ggtgtctata tgcatgtacc cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc
      361 tctgtttctg agaccactga ttcccctatc actattactc atactttcac tgatgcaaat
      421 ggaaaacagc tacttcctgc attgacttcc tataataaag tcagttatcc tccaacgata
      481 aaccactact ggttaattcc taacaaaaca ttatggacat cggaaaccgg aagcagtgtg
      541 ccgctttcta tcttttccga tatgactgat cctacaaaag gtgatcagtt aaaacagatc
      601 actgatattt tgaatcatgc tttgacagat ataaaggacg gtggaagcgc acaagtttct
      661 tatgtatatg aaaaagatta ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg
      721 acaaaagttt cgatatctga tctggtagaa aggctgaaga acgcagatgg aacacaagga
      781 gatatgtcca aagtaatttc aaacaatggg aatatcgtgg atacgactaa tacaggaaca
      841 tctactattt ccttgtccta ttttgatcca attgctttaa aaacaatgac tgcatctgct
      901 gtgatccatg ttcgtgctga tcaaacagct atcaaaggaa aagatcttca cttaagaaca
      961 ggagagagtt acgcccctaa agatcttgtt gaatatgtaa caaaaagcga tggaacttca
     1021 ggaaataaag aagaaatcaa aatgaacggg acagtagata ctacaactcc gggagaatat
     1081 cccgttgtat taacttatat agactcgaaa acatcaaatg tggcagaaac aacagctgta
     1141 gtttatgtag aagcatctgc tgcttcatcg actgtcatca ctcattttat agatagtcaa
     1201 acaaataaag aacttcaagc agaaacacat atgagtagtg taaatcgtat tcctgatttg
     1261 cgcctacctg ctgaattttc tgatcgagtg ccaaatatag atttatcgac ttatgaacaa
     1321 aatgggaaag tcacttctat ttctgattgg atgcaagcaa acggattaga cagaaaaagt
     1381 cactggtcgg agatccttga agtacttcac tcagaactga tgaataatag gaagcaatca
     1441 ggaggggaaa ttacctttac ttatgtttat ataccagaca atcgttcatt agaaggtcat
     1501 gatcttattg ttcatccaaa cgaaaaaata aacaaagaag agttgatcca atcagcaaca
     1561 aattccttag gtaaacccgt accgttaaag gatgtatcgg ttacaatcaa tgaagtacca
     1621 ttggaggatg gttatttgac caaacaaaca ggtgatttta cgattatgta tagttatcat
     1681 gacccgtatt cactccaaga actccatgct cagtcactat tacatgtttt accattaaac
     1741 aaacagatag atcaaaaaga acaaaaaaga cgagaagaaa gcttgtcaga aaaaaaacaa
     1801 caaatggctc atgcagtttc tccaacaggg aaagtaaaac aaacaagaag cctagagtta
     1861 gcaagaatat ctagaaaaat ctcagaatct aataaaacga taaggcaaga agctcatttg
     1921 gccccacaag tttctggatt ggaaaatgaa attttagagc ctaaaataaa acaacaatca
     1981 gaaaaagaat ttgttgctaa acagtcggaa gatcagaagg aatcagatga ccagaaggaa
     2041 acgaagaaaa aacgcttgtc aactattgat tccaccgatg agcatctgca gccgcaagta
     2101 ccacttccta gtccgggaac acccgcacct ggtggaggta ttccttcttc tgctttagga
     2161 gatttcatgc gaggtatggc aggaacatgg gtatacgcta atcgaaatga atttgacgca
     2221 gatgactggt tctgatttat atctctatct gttatagtca aataagtagc tgagtcatat
     2281 gttatttggc ataatggaga cgaggaggga tagaaaaatg ttagataaaa cgttacctta
     2341 cgcagaaatc tggatgacaa gaccacttca acaaaatgtt ccaatcacgc ctttagcgga
     2401 aggttttgaa tttcgatttt atcaaaaaga agatgaatct gcttgggcgg agattgagac
     2461 ttccgttggg gaatttcaaa cgacagatga agcacttgag tattttcaac aaaactttgc
     2521 tccttatcct gaagaacttg aaagaaggat gttttttgta gagacaaaaa aaggagaaaa
     2581 agtagcaacc tgtaccgctt ggcgaaaaaa atcaagagag caagtgtatc cggtttttca
     2641 ttggttagca gtgaaaccag attaccaagg aaaagggctc gcaaaagcat tgactgccag
     2701 agtcttgcaa gaatttccta ccctccatac ggacggacca atctatcttc acacacagac
     2761 atggagccat caggcgattg ctctttataa aaaaatgggt tttactttta ttgctgaaaa
     2821 tctagacggc acacctaatc cagattatga aaaagttatg caggttttaa gtaaattaga
     2881 taaataacga aatggtgaaa taagtcctca gtattgggat aaaaaacgaa atagtcattc
     2941 ctcatatctt acttttttga gttaatacta tcggataact aatggcatgc catacattca
     3001 aaaaagaagg ggcagtgaca aaagtattgt cactgcccct tctcttgttt tttagcatgt
     3061 tttatcgttt gattttttta ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt
     3121 ttcgactata gaggtaacca aatacactga aaacaactgc tccaataaaa ttaacaaata
     3181 aatccttcat agtatccatg attcccacgt ctaagtaacc attttggatg attgttttat
     3241 ctgttttacc atctgcattt tgactttcaa tgattgtccg agtgatatct ggaacatgat
     3301 agacagtgtt tccgtcttta cttaactcca cactattgat ttgctggatg atcgtatctt
     3361 tttgcatatc gaattgcagt aatttatctg ctgaaaattc cacaaactcc cacataacac
     3421 caactgtcat cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg
     3481 tttttgtatt tttattgagc aaataaacaa gggaaaagcc gacaccccca gctaaaaatc
     3541 cgctgaatgc atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa tcacttaact
     3601 ctcctaaaac tgttgaacta aaaataaaag ataacaaaat gatttctagc atatttggaa
     3661 tctgtatctt taatatcttt tccagaaaat aagggatttc aaataaaatt aatgtcagta
     3721 ataaaagcat tgtatttccc cagtgttggt tccagacctg atccactagc ataataacga
     3781 cgatgatacg taataaaata tagattccgt ttgtgattct acctgcagtt tttgtactgt
     3841 tcgtaaaaaa ggttttcaaa tgattcataa aataactcct tcatgtatat ccttaattat
     3901 actaagaagc gtgtgtttac caaagctata tgacttaaag aaaaacagag ttgatattgt
     3961 gaaaaaaatc acttaatata gtataaggag aaaaggggga tacaaatgaa aaaattagtt
     4021 gctggtttat taacagccgt tgcacttctt gctatgagcg catgcggcgc agatcagaaa
     4081 aatacagcgc agacaagttc tagtacgaag gaatctgccg aagtatcatc aggcgcttct
     4141 gaacaagtat atacagatcc aaaagaaatg aaagaatctt atgatgtcgt gattattgga
     4201 gctggtggtg cagggatggc agcagctatc gaagcaaaag atgctgggtt gaatccagta
     4261 attttggaaa agatgcctgt tgccggagga aacacatcta aatcatcaag cggtatgaat
     4321 gcttcagaaa caaaatttca aaaagaagaa ggaatcaaag actcgaatga tgccttctat
     4381 gaagaaacgc taaaaggtgg tggaggaacg aatgataaag aaatgcttcg atactttgtc
     4441 gatcactctg ctgatgcggt cgattggcta gatcaaaacg gtatcaaact ggacaactta
     4501 acgatcactg gagggatgag tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt
     4561 ggtggatact tagtggaggg gttattgaat aacgtccatg agcgggaaat ccctattttt
     4621 gtcaacgcag atgtgacaga cattaagaaa aatggggaaa cggtcaatga agtgactgta
     4681 catgttcaag gagaagaacc aaaagaggtc accggaaaag caatcgtagt taccacaggc
     4741 ggttttggtg ccagcaaaga atttattgaa aaatatcgtc ccgatttagc tgattatatt
     4801 acgacaaatc aagaaggatc aactggagat ggtatcacca tgattgaaaa attaggtgga
     4861 caaaccgttg atatggataa aatacaaatt catccaactg tgcagcagga tgagggggtc
     4921 ttgattggtg aggttgttcg cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga
     4981 tttgtcaatg aaatggatac aagggataaa gtatctgcag caattacagc attgcctgaa
     5041 aaatcagctt atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt
     5101 tatgaccaaa aaggatatgt cacaactgga aagacaatgg aagaattagc agaaaaaatc
     5161 gatttaccaa aagaaacact agcaaaaaca gttgatggat ggaatacgga tgttgcggag
     5221 aaaaaagatg ctcaattcaa ccgtgccaca gccatggaac atgacttatc gtctccgaat
     5281 tattatgcga tcaaaatcgc accaggaatc cattacacga tgggcggtgt gaaaatcaat
     5341 acgaacactg aagtattaga taaagaaaac cagccaatta gggggttata tgctgctggt
     5401 gaagtcacag gtggcttaca tggaaacaac cggatcggtg gaaattcagt tgcggagatc
     5461 attatttttg ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga aaatgaatga
     5521 aatagaacgt aaataaaaag aggttgtgaa atcagcgttt tgagctaata ccgagggtca
     5581 gctgattgaa cactgtttag tgaagaggtt gtgaaaaag
//
LOCUS       DAZAOG010000158         5601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000158 DAZAOG010000000
VERSION     DAZAOG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.160"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..769
                     /locus_tag="ACHDLD_002436"
     CDS             68..769
                     /locus_tag="ACHDLD_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN3364104.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            766..1887
                     /locus_tag="ACHDLD_002437"
     CDS             766..1887
                     /locus_tag="ACHDLD_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN3364105.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1902..3134
                     /gene="pepT"
                     /locus_tag="ACHDLD_002438"
     CDS             1902..3134
                     /gene="pepT"
                     /locus_tag="ACHDLD_002438"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN3364106.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3266..4174
                     /locus_tag="ACHDLD_002439"
     CDS             3266..4174
                     /locus_tag="ACHDLD_002439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3364107.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4209..4481
                     /locus_tag="ACHDLD_002440"
     CDS             4209..4481
                     /locus_tag="ACHDLD_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN3364108.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4492..5538
                     /locus_tag="ACHDLD_002441"
     CDS             4492..5538
                     /locus_tag="ACHDLD_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN3364109.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1821 a          971 c         1215 g         1594 t
ORIGIN      
        1 atttttttat tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag
       61 tgaagctatg aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc
      121 tgaatccgct cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat
      181 attaagagga aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc
      241 agcacgtaag caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa
      301 tggtctagat gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg
      361 aggttcactg atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca
      421 agaacgtttg atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga
      481 aaatcagtat aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat
      541 cattgtagcc gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg
      601 gccatttctg cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca
      661 acaaagaaaa gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa
      721 acaaatcgag caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga
      781 cgcactttca tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac
      841 ccagtcggac tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta
      901 gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct
      961 aaacatcctc caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa
     1021 atgtatgcag atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat
     1081 attatttggg atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt
     1141 tatttagtaa agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat
     1201 catgcacaga aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat
     1261 accggtacaa gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa
     1321 ccagcgattg ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt
     1381 ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag
     1441 gaacctgctt atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga
     1501 gtaggtgaat tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg
     1561 ttccaactag atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc
     1621 attgcgattt gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca
     1681 gatgtctata tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg
     1741 ttgattgcaa ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag
     1801 aaatttgaat cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca
     1861 aatacaaacc catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc
     1921 cacgtttttt acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc
     1981 catcgactca gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag
     2041 gcttagctga tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca
     2101 atgttgatca tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta
     2161 atgcagtagg agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac
     2221 tagatgcgga agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg
     2281 aaggccacac attgatcaca acagatggca gtacattact gggagcagat gacaaatcag
     2341 ggattgcgga aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg
     2401 gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg
     2461 atgttgagca attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt
     2521 tgcagtatga aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac
     2581 atccagggac ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata
     2641 atcagcttcc agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt
     2701 tgatggcatt ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg
     2761 atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc
     2821 agcgatttga tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag
     2881 aaatcattga aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg
     2941 gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca
     3001 tgggtattcc gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat
     3061 ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga
     3121 acacaaagag ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg
     3181 aatgtcatct gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt
     3241 ttgaagagta aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc
     3301 agggaaagat gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca
     3361 gccagaatta tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt
     3421 caagaaagca gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat
     3481 tcatcatatc gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc
     3541 aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc
     3601 tgctgacgtg atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga
     3661 agtcattatc tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca
     3721 agaagaaaaa cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct
     3781 gaagaagaaa aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc
     3841 ccaactgttg acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc
     3901 agcgacccta gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg
     3961 atttattttt tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac
     4021 ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac
     4081 ggatggagac acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac
     4141 agtttattca ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa
     4201 gaagagtaat ggatgtattg ttacacaatc ctttgtggtt ttaccttttc attgtgaatt
     4261 tttacttatt ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg
     4321 ttcctgagaa taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct
     4381 ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag
     4441 gtgtacttgt tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct
     4501 aaaggaacga aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt
     4561 atttattatt taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt
     4621 gttgttgttt tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc
     4681 tcaatcaaag aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg
     4741 acctctagct atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg
     4801 gagatttatt tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca
     4861 ctccatatga ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc
     4921 caattctctc ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc
     4981 cttttgacga tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa
     5041 atattttttg tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg
     5101 gatacatgca atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac
     5161 aggactgcgc tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta
     5221 cttccttatt tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg
     5281 tttatcagct ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc
     5341 tttgaatttg tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct
     5401 tcgcttttac tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat
     5461 gtgattgctg aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat
     5521 aaagaaaatc aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa
     5581 cgttatggat aagataattt t
//
LOCUS       DAZAOG010000159         5571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000159 DAZAOG010000000
VERSION     DAZAOG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.161"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..551)
                     /locus_tag="ACHDLD_002442"
     CDS             complement(42..551)
                     /locus_tag="ACHDLD_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFN3364110.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(752..1621)
                     /gene="scrK"
                     /locus_tag="ACHDLD_002443"
     CDS             complement(752..1621)
                     /gene="scrK"
                     /locus_tag="ACHDLD_002443"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3364111.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1641..2762)
                     /locus_tag="ACHDLD_002444"
     CDS             complement(1641..2762)
                     /locus_tag="ACHDLD_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN3364112.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2764..3159)
                     /locus_tag="ACHDLD_002445"
     CDS             complement(2764..3159)
                     /locus_tag="ACHDLD_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3364113.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3178..3975)
                     /locus_tag="ACHDLD_002446"
     CDS             complement(3178..3975)
                     /locus_tag="ACHDLD_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3364114.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3977..4765)
                     /locus_tag="ACHDLD_002447"
     CDS             complement(3977..4765)
                     /locus_tag="ACHDLD_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3364115.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4779..5255)
                     /locus_tag="ACHDLD_002448"
     CDS             complement(4779..5255)
                     /locus_tag="ACHDLD_002448"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3364116.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5327..>5571)
                     /locus_tag="ACHDLD_002449"
     CDS             complement(5327..>5571)
                     /locus_tag="ACHDLD_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3364117.1"
                     /translation="LGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQ
                     KFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1716 a         1214 c          820 g         1821 t
ORIGIN      
        1 tcatcaaaac aaccattgtt ttgatttctc atgaaatttc tttatacttt tctccgatta
       61 taaattccta accccttgag aaaattacgc atatatttat ctccgcaagg cttaaaattt
      121 cgttggcctt cttttctcaa aaatgccccg atttcgcctt ttgatggata gatttctgcc
      181 tcagcaaaaa cgtctaaaat atcatcagtg gtcatagaca aggcgatttt taattttttt
      241 agcaacacat tgttgatata tttgatttcc ttgctttttt gcggcgtgtc tactatcggc
      301 ttatcttttt gcggaccacg cgccaagaga atcaggccat ttaggaatgc gtcaaaaact
      361 tggttgttga tggttttttc atacacatta ttttctgcat tatctttgtt cttatcctgt
      421 actttggtca gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc tagctcaaaa
      481 gctttaatca aatccgtatc tttcaaatca atcgcataac gcaatcgcaa taacctatca
      541 ttgttattca tttgttcctc cgaagtattt agtctttact ttcttacgta ctttttgtct
      601 tgctaagatt tttaggatca atgataaaaa ccgcatcact tatgataagg cgctgggtat
      661 catgagtgaa tgatattgct gaatacaaat agtccgacca cattaagttg atcggacttc
      721 ataagcattt tattgactaa tacttttttg cttaagaatt gattctaaca tcttttgcta
      781 aagttaagca tcctatagtt cctgcattat ctgctaactt aggtgtgaca atgtattctt
      841 cgatatttgg cgtatcgaca tagccgttaa ttagttctaa aaaacgtctt tgtactttct
      901 ttttcagatg ttcttgttgc atgacaccac caccaaaaat tataatatct ggtgaaagca
      961 tcagggtcgt attacaggca cattgtgcaa tataatccgc ctcgatttcc cagaacggat
     1021 cttctataga aagatcttgt cccttggttc ccgtacgcat ctcgatagcg ggtccagatg
     1081 ccaacccttc gagacaatca tgatgaaaag gacaagtacc agagaaagta tctcctgaat
     1141 gtctcttaac taacgtatgc cccatttctg gatggctaaa cccttcaaca aaaatcccat
     1201 cttgtatagc tcctccacca attccagttc caacagtgta ataaacaaca ctggataaac
     1261 cttttccatg acctgaaaca tactccccgt aacaagcagc attcacatcg gttgtccagg
     1321 caattggaat ttcgaaatgc ttattcaacg ttccaacaaa atcaaaattt ttccaggcag
     1381 tttttggagt agaggtgata tgtccatagg tctttgattc acgatggata tcaatcggtc
     1441 caaatgaccc tactccaatg ctttctaatt gttctttata gtgattaaag aaatcaatta
     1501 ccaaactcat tgtttcattc ggtgtagttg taggaaaact cactctttct aaaaccttta
     1561 aatcatcatt gccaatggca caaacaaatt tagtcccacc tgcttctatt gagccataca
     1621 tttcttactc caccgtcctt ttatctagga attcgttctt ccctgtctaa tagtttttcg
     1681 aaaaactcat gtggttcctt ttggtaccaa taggcaacag aagaaatatc gtcttgacgc
     1741 tcataaagct tgatgtcatc atttcctagg gtttggaaag taacacgtaa attttctttg
     1801 aaactaatgg ggtcgggtaa atgccagcgg tacaacccat gcatcggtaa agcatcatcg
     1861 ccaaaaccat gaactggatt tggattaggt tttccagcac taaaataatc ccgggtgtga
     1921 tctcttttag attgaaaagg atagcccata aatagcgtat taaaattttg agcatatgcc
     1981 ctttctccaa agttcttttt atggaaagcc caagcgccgc caaaataatc ttctgctcct
     2041 gtagatgtta ttgtagggaa atcttcatct ccatctatat aaaacttaaa ttctccttcc
     2101 ccccaccagt atctttccaa tgcgcacaaa gcaaagtagg ttcctacata atacccagtg
     2161 cctttgattc catcaataag ggtgtaatct tttccttttg tcgtgatggg ttcacggttc
     2221 catttagcat gaaaatacat cgcatcatcc ggtacttcag tcaatgcata attaaatgta
     2281 taaaaaatgt gcttaataaa ctcaggatgt tcattcgtta aagtaatctt agcttttttt
     2341 cgaaatggca tctcaaaata gcaattcatc cctgatgttg gattcactac gattggtaag
     2401 gaattcactt cgcatcttgt cccaaaccca ttacaaaaaa aatctcctaa tggactttct
     2461 acagaaggag tggattcatc atcccaataa atacgcaaaa ttaaatcacg taagacgaaa
     2521 ctcccagcga ttgttttgtc tgtcactgtc atccaaaaat gacgtaaaat tccagggcct
     2581 tccatttctg ctaaagtaat tgtctctcca gcatttatcg gaacagaagg tgaccctttt
     2641 ctcccaattc ctagatgact cgctgtcatt gccgctttcc ctttttcacc atttttattt
     2701 tctggtgaaa tagttctact ttcctctccc ttgtggagcc atatattatt taaacaattc
     2761 attttattcg tcctctatcc tttcttcact tttttgatta ttcatataga tgatttgttg
     2821 ttgtgcctcc gcaataattg ctccaatatc taaattatca tttcctaact gcaaagcgat
     2881 tgacattgcc aagggaagat tcattcctgc aattacgtgc actctgtcag aaatataggt
     2941 aaaaaacttt tgatttacac ttcctccagc catgtcagtt aaaacaatta tttcgtctgt
     3001 agtatccact aactctttaa tttgttcatc tatatcatca atttcttcga catatgcaca
     3061 taagctacta atttgattat gcatacctgt taaaaactct agtgtctctt taaaaccctc
     3121 agctagtatt gaatggctag caatgatcaa tcttctcata attcaaatcc ttttccttta
     3181 cgctaatatt ccaaatgcag ccataaccat cgcaaaaaca ataacgagta gaatcaaaat
     3241 ggtcatactg atttttttct tactgagtag ataatatgct gcagcagtaa taattactgg
     3301 aattaatgtt ggtaaaattt tgtccaacat tggttggatt ttcatactaa cttcaccaaa
     3361 agtgaattta agtggcgttg aaacactgat aactgaagca attaatgatc caactactgt
     3421 tagccccaat actgaagcag cttcagttaa ataattaatc ttttcaccgt attcagtaac
     3481 aatcttcatt ccctgcttat aaccaaattc aaagcctctc attcggaaaa agaagattac
     3541 catgattaaa atgaaccata aaaagattcc tacaggatta ttttctaaag ccatataagc
     3601 cgcaattgat cccatgatgg ttgggatcat cgtcatcaaa agagagtccc caataccagc
     3661 aattggaccc atcgtaccaa ctttaaagtc attaatcgca tcttttgctt ctaatccttg
     3721 cttttcctcc attgcaacac aagcacctaa aataatggct gccagccaag gctgtgtatt
     3781 gtaatactga aagtgattat ttaaagcttc gatgtattca tcatcatctt tgtataattt
     3841 tcttaaaata ggtgacaatg cgtaagtcac agatgcacct tgctgtgttt gataattaaa
     3901 ggtacaaact gacatcaacc aacgatagtt cgctttccta agatcctttt tcgttacttg
     3961 atatttttcg ctcattttat tcatcctccc caataaaccc aacatcagaa gctcccgcag
     4021 aagcagtgat ttgtgcatct ttttcagctc tatgatacac attaaatgct aaagcgaatc
     4081 ctatgatcgc cgctccaata ataggtacct tcaaaaaagc ggctaagaaa aatccaacaa
     4141 gtaaataact aatgtatcga cctgtaggca taaagcgtaa caacatcgca ataccaacag
     4201 caggcaacat ttttccggct aatgttagcc catccataaa ccaattaggt aaaacatcca
     4261 aaacgtaatt aacagcagac tgtcctaaag taacggcaat aaatactgga attgctgcag
     4321 ataaaccaaa gaaaattggc cccaaccaca aaacactatt cattttctta aattctcgtt
     4381 tattcgcata ttcctgtgat ttgcgaacaa caaaaccatt taagattttg acaattacat
     4441 ctagttgcac ccctaacata cctacaggta gtcctattgc taatccggct tctactccaa
     4501 gctggctacc aaatgccgtt gcaataattg cgcacgttcc atagtccggt acagatgagc
     4561 caccaaaacc ggcaactcct aatgacatca attgaactgt accgccaact atcaaacctg
     4621 tttttatatc acccatcaaa attcctgtga ataatcccca aaaaactgcg taatttacaa
     4681 agatcatcgg aatcccataa taatcaacat gtttaataaa tgctaatata gtcagcataa
     4741 taatttgaaa aatagtaaaa tgcattatcc ttactccttt atcctaattt tgattgaatt
     4801 tcctgtgcct tatccgccgg aacaaattgt gtaacaattt ctacaccaga atccaacaat
     4861 tttttatacg tgctgatatt ttcatctgat gcataggctc tagaagatag ctgtaatgca
     4921 ttatctttaa caacagtacc gccaacaatt agtcggtcaa ttctaatacc gttatctact
     4981 aaatgaagga ttgtttccgg ctctttcacg atgacaaata ctttttcctg atcgtatttc
     5041 ccctttttaa aattcgcaat cgctgtttct tcagtgataa ttgatacagc cattccagca
     5101 ggtctagcta aacgcatact tgatttttgg atgtcgttat ttgcgacaga atcatcgatc
     5161 accatcactc tgtttgcttt agacattggt gcccattgcg tgacaataat cccatgcaat
     5221 aaacggttat caactctcga cataacaata ctcataaata cttcctcctt tatctttcat
     5281 tttcttacaa gcaattacca tgccaaaaca aaaagaactt gataaatcaa gctcttttta
     5341 cataatcata aacgtattta acctcatgaa tcgggatgtc gacactataa taatgttcaa
     5401 cgacactgaa agattgctta aaatgtgtct gaaatttttg aaattcctca ctcgtttcat
     5461 tgaaataaat ttcatcttcc aaagtatttt gttttactaa tcgttcaatt aagcaactaa
     5521 tgtgaacata taatccgaaa cacgtattat tatcaaatga aattcctaga t
//
LOCUS       DAZAOG010000160         5564 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000160 DAZAOG010000000
VERSION     DAZAOG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.162"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2080
                     /locus_tag="ACHDLD_002450"
     CDS             <1..2080
                     /locus_tag="ACHDLD_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFN3364118.1"
                     /translation="KYFKTDIPIHKLRDLSGTDLEGTSAFGLKKTFEKLSFDCMAIQA
                     DSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWIADPANGKNRKTIEAFQKE
                     WTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFHIVLASIFITFFGIGSSYY
                     FQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSYLLIILGQRMSMAIMLQYF
                     KHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSIFLDIGMVLLVGVTLAIQN
                     STLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVNSSIIESLKGIETIKAYSG
                     EEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLISSALILWLGSYFVMDNRIS
                     LGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNEIFAIDSEKSNQSFEYMLS
                     EQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIVGVSGSGKSTLAKLFVRFY
                     EASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTIIENLIFGLDSPPSFEQII
                     EVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLALARALLKNPDILILDEATS
                     GLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILVLHEGHLVEKGTHDELRFA
                     DGIYQKLWEI"
     gene            2049..3464
                     /locus_tag="ACHDLD_002451"
     CDS             2049..3464
                     /locus_tag="ACHDLD_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFN3364119.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3497..4531
                     /gene="sppA"
                     /locus_tag="ACHDLD_002452"
     CDS             3497..4531
                     /gene="sppA"
                     /locus_tag="ACHDLD_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN3364120.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4533..5054
                     /locus_tag="ACHDLD_002453"
     CDS             4533..5054
                     /locus_tag="ACHDLD_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN3364121.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5051..5437
                     /locus_tag="ACHDLD_002454"
     CDS             5051..5437
                     /locus_tag="ACHDLD_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN3364122.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2002 a          759 c         1051 g         1752 t
ORIGIN      
        1 aaaatatttt aaaactgata tcccgattca taaattacgg gacctatctg ggactgattt
       61 agagggtact tcagcatttg gattgaaaaa gacatttgaa aaattgtctt ttgattgtat
      121 ggctattcaa gcagatagtg agatctggaa agaaagagat cttatcttac cattaattgc
      181 acatatattg gttgataaca gctatatgca ctacgttgta atttataaaa cgaagggaaa
      241 cgtactatgg attgcagatc ccgctaatgg aaaaaataga aaaacgattg aagcgtttca
      301 aaaagaatgg acaggtattc ttttattacc tatgcctaga gaaacttata ttcctattaa
      361 agaaaaagta gctggattaa gttctttttt gcctataatt tggaaacaaa aaggattagt
      421 attccacatc gttttagcat cgattttcat tacgtttttt ggcataggaa gctcatatta
      481 ttttcaaggt attcttgatt ttttcatccc taatcaagca cgttcaactt taaatataat
      541 ttccattggc ttgatcgctg tctatttatt tcgggtattg tttgaatata gtcgtagtta
      601 tcttcttatt attttaggac agcgaatgag tatggcaatt atgcttcagt atttcaaaca
      661 tgtgttaact ttaccaatga atttttttgc tacgagaaaa tcaggggaaa ttatttctcg
      721 ttttcttgat gccagtaaga ttattgatgc tttagcaagt gctacgttat caatattttt
      781 agatattggt atggtgttgc ttgttggggt tacgttagct attcagaata gtacgttgtt
      841 tttaatcact ctatgctctt tgccttttta tgtagttgca atattcgctt tcgtgaaaag
      901 ctatgaaaaa tcgaacaggg aagaaatggc agccggtgca gctgtaaatt ctagtatcat
      961 agaaagtctt aaaggaatag aaacaattaa agcatatagc ggtgaagaga aagtatataa
     1021 ccgtatagac catgaatttg ttaaattaat gaaaaaatca tttcgtacgg ttacgcttga
     1081 taatattcaa caaggaatta aacatttgat tcaattaatt agcagtgcat taattttatg
     1141 gctaggttct tattttgtta tggataatag aattagtcta ggtcaactta tcacatacaa
     1201 tgctttacta gtatttttga cagatccttt acaaaacatt attaatttac aagtgaaaat
     1261 gcaaacggct caagttgcaa ataatcgttt aaatgaaatt ttcgcgattg attcagaaaa
     1321 atcgaaccag tcttttgaat atatgctttc tgaacaatta tttcaacaag atatcactgt
     1381 aaaaaacgtt tcattctcgt atagcatgaa ggccccaacg ttgaacgaaa tttcatgtac
     1441 aattccttcg cacagtaaaa cggctatagt tggagttagt ggttctggaa aatcaactct
     1501 tgcaaagtta tttgttcgtt tttatgaagc atcagaagga tcgatccatt atggaaacat
     1561 taatcactta gacattcctt ttcagatatt acgcgatcga gtgacatatt tgcctcaaga
     1621 gtcatttttc tttagtggaa caatcattga gaatcttata ttcggtttag atagcccacc
     1681 aagtttcgaa caaataatag aagtttgtga gatagtccaa ctaaatgaat ttattaatca
     1741 acagccatta cgttacgaga cattgttgga agaaggtgga aataatcttt caggagggca
     1801 acgacaacga ttagccttag cacgggcttt attaaaaaat ccggacatat taattttaga
     1861 tgaagcaact agtggattag ataccttgtt agaacatgca attatgaatt atctcctcaa
     1921 tttaaaagaa aaaacgatta tttttattgc gcatcattta ccaatagcaa aagcatgtga
     1981 tcaaatttta gttctccatg aagggcattt ggtagaaaaa ggaacgcacg atgaacttcg
     2041 ttttgccgat ggtatctatc aaaaattatg ggaaatctag gagagggaaa caatttatgc
     2101 agaataataa ttggttcgaa cattctagta tctatactaa acaacatcat agcttctacc
     2161 gttgggtgtt ataccctgta attctttttt tatgtattat tggattgttt ttaacctttg
     2221 ctaaaaaaga ggttgttatt cggacaacgg ctcaattgaa ccccgaaaaa atagagaagt
     2281 tacaagtgcc attagaagct aaaataatgg aaaacttcct aaaagaaaat caatttgttc
     2341 ataaggacga tgtgttggtt agattggatt gttctttaat agagaatgaa aaggctcaga
     2401 tagaacaaga aaaccaacgt attactcaac aaataaagat ggctcagcta tttattgaaa
     2461 gtataagtaa aggaaaaaat ttgttttcaa cggatgacag ttttggctac agtaatcaat
     2521 taaagagcat gttgtcagaa aaagaatcac tccgctacgc tttgaagcaa agtgaattaa
     2581 atgatcaaaa gcaattagaa gtatacgaaa agacaaaaag acaactagaa aaacaaattg
     2641 agagttcaga tagtaaatta caagaatggc aacaagtaca ggtagcttgg agtaataatc
     2701 aatcattaaa agatttttca aaagaaatga tggcaaacta tgagaattgg caagaacaac
     2761 taaataatgt ttctgatgat caaaaaaatc aagtgaaact gacaatttca gcaagcataa
     2821 atgaacaaat tgagcaacta aaaaaagaag tagaacagta tcagtcagaa aaagctaaat
     2881 tagttaaacc aactacttct gagaatgaca gaattagtca aacggaaaaa ggaaagcaag
     2941 agctagaaca agcaataaca actgtaaaac aaacagttac agagctaaat gaaaaacaag
     3001 agaagaacca gtcagttatc aaaagtttgg acgatcaact ttcaaaagga atactaaaag
     3061 ctcctgtatc tggaacggtt catttaaatg aagaaacaaa gggacaagca gaactagcca
     3121 aaggaacagt gcttgcagaa atttatccga tccacgaaaa atcaaaaatg aaatttacag
     3181 cacttttacc tgcgaatgaa agtacctata ttaaagaagg gatgaaggtt cattttaaat
     3241 tggatcaaaa aggtggggct ccgattacga tcgacggaac tttagatgaa atttcagaaa
     3301 attctactgc cactgagaaa ggtgtttttt atgcagtgaa aggaacgtta aaaacaactc
     3361 aatcgtttcc gtttcgttat ggattaactg gagagttgtc tttaattgta ggagaaaaaa
     3421 catattggca acaaattgtc gatgtgtttc taaataaaaa ataattattt ttactaagaa
     3481 tataggagga gtttatatga ataaaagaag atggattgct gtgggagtag cgtgcggatt
     3541 attgttacta tccattatcg tcgcgctaat tccaggaaaa gataaagagg aagcatcaaa
     3601 tacaacccta acaggaataa ataagatttt ttacggatct aacgaaatca cagaagaaac
     3661 gttagaagaa ggcgcatcaa ataaaaagat tgtcaaacta tccgtcaatg gagttattgc
     3721 agatacaggt gaaagcaatc tttttagtag ggaacaatat aaccatcaga attttttaac
     3781 acagctaaag aaaattcaag aagacaaagc ggttaaggga gtgttattag aagtaaattc
     3841 acctggggga ggaatatatg aaagtgctga gattgctaaa gaaatggcaa aaattaaaaa
     3901 gctagatatt cctatctata ctgcatttaa aaatacagcg gcaagtggag ggtactacat
     3961 ttctgctggt tcagataaaa tatttgctac ggaagaaaca acaactggat caattggtgt
     4021 cattatttct ggtttaaatt attctggctt attagaaaaa cttggcgtaa ctgatgcgac
     4081 gtataaaagt ggtgcattaa aagatatgat gtcacctcaa cacaaacctt cagaggaaga
     4141 gaataaagtt atacaggaat ttgtgatgag tgcttatgat cgttttgtca atgttgtagc
     4201 aaaaggaaga aatatggata ctaatgcagt gaaagaactt gcggatggaa gaatttatga
     4261 tggtaaccaa gcagtagaaa atgggttggt ggatcaaatt ggttactctg aagatgcatt
     4321 agattcccta aaaaaagaga agaaattaac tgatgcgacg gttatagaat ataaaaatga
     4381 cacaactggc tttgcttctt cttggttagg taataagatt gctgaatggc aagggctaaa
     4441 ggcaactaca tcagatcgtg taatttcagt ttttgaaaaa cttggatttt ctgatactcc
     4501 aaaaccaatg tattactatg gagggctata aaatgaataa gaagagtggc attgaaactt
     4561 tgcctaatta tgtatttgcg ggattttggt tacgattttt tgcttttctt attgatttaa
     4621 tttgtattag catcatcacc catgctacta taggtctctt ttacaaaatc atgggactgg
     4681 aatctgacaa atccattatt actttttatg gtatagcgtc attagctatt tatttaacct
     4741 attttgtact tttgactaag ctgaattcag gacaaacaat tgggaaaatg atttttggga
     4801 taagagtagt gagctttgag gaaaaagagc taagttggca aactatcttt attcgtgagt
     4861 gtatatgtag gtttatatta aaaaattttg tatttcaaat tgggtatttg gtggctgctt
     4921 tcacgcccaa gaaacaacat attggggatt atttttctaa cacgtcagtg gttgttattc
     4981 gttcattaga agctattaac tatggtcaaa aaatttctaa ttctaaaaca cttaatctgg
     5041 agagaattaa atgaataaga gaaaaacaga gtttattttg attttaatta gtggaataac
     5101 agggatttta tgtagtcttt tagcattgat gggtggtatg tttctatctt ctattgattc
     5161 aactacttta aatactacag aactaaccag taaggatata gatatgttag tcaatctaag
     5221 cgggaatgtt gttttatttg gaggaattac cctagttttt tcgataatga ttttcataat
     5281 aggtttttta gtaagaaaaa atcaaaaaac agtggtctat ggtattagtt tactcatttt
     5341 atctattatt ccattttttc ttttaacatt attgtgggta attcctggag ttttaggtat
     5401 tattgcggga ttgatgcttc taataagaaa atcttaatta ggcaatgtgt agacaacgtg
     5461 aaacgattaa tcaattaaaa gctaaatacg ttttgagaaa tgataacttg tagtcgatgg
     5521 aagacatgta gataaaaaaa ctacgtgtct tttatttatt ctaa
//
LOCUS       DAZAOG010000161         5560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000161 DAZAOG010000000
VERSION     DAZAOG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.163"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..2009)
                     /locus_tag="ACHDLD_002455"
     CDS             complement(60..2009)
                     /locus_tag="ACHDLD_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3364123.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="ACHDLD_002456"
     CDS             complement(2051..3109)
                     /locus_tag="ACHDLD_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFN3364124.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="ACHDLD_002457"
     CDS             complement(3228..3683)
                     /locus_tag="ACHDLD_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364125.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="ACHDLD_002458"
     CDS             complement(4267..5070)
                     /locus_tag="ACHDLD_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3364126.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1632 a         1101 c          768 g         2059 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga
//
LOCUS       DAZAOG010000162         5534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000162 DAZAOG010000000
VERSION     DAZAOG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.164"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..988)
                     /locus_tag="ACHDLD_002459"
     CDS             complement(<1..988)
                     /locus_tag="ACHDLD_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFN3364127.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACHDLD_002460"
     CDS             1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACHDLD_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN3364128.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ACHDLD_002461"
     CDS             1813..2967
                     /locus_tag="ACHDLD_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFN3364129.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ACHDLD_002462"
     CDS             3182..4150
                     /locus_tag="ACHDLD_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN3364130.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ACHDLD_002463"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ACHDLD_002463"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN3364131.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="ACHDLD_002464"
     CDS             5180..>5534
                     /locus_tag="ACHDLD_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFN3364132.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DAZAOG010000163         5401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000163 DAZAOG010000000
VERSION     DAZAOG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.165"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..773)
                     /locus_tag="ACHDLD_002465"
     CDS             complement(<1..773)
                     /locus_tag="ACHDLD_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364133.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYS"
     gene            992..2212
                     /gene="sstT"
                     /locus_tag="ACHDLD_002466"
     CDS             992..2212
                     /gene="sstT"
                     /locus_tag="ACHDLD_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN3364134.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            2359..2994
                     /locus_tag="ACHDLD_002467"
     CDS             2359..2994
                     /locus_tag="ACHDLD_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN3364135.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            3185..3925
                     /gene="truA"
                     /locus_tag="ACHDLD_002468"
     CDS             3185..3925
                     /gene="truA"
                     /locus_tag="ACHDLD_002468"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN3364136.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(4070..>5401)
                     /locus_tag="ACHDLD_002469"
     CDS             complement(4070..>5401)
                     /locus_tag="ACHDLD_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364137.1"
                     /translation="TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRAS
                     LGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLF
                     LSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSD
                     MLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQ
                     LFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLS
                     GVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEIN
                     PTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIP
                     PGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         1788 a          820 c         1037 g         1756 t
ORIGIN      
        1 gaatatggtt tagaaaacct cagcgctgta tctgtatttc ctctgatgat gagtgaaatt
       61 tgtgtcatca aaataatcaa accgaataaa acaatcaagc tgataaatac ttgaggaagc
      121 ttatagcgga ccttttcttt ccagttccat tctttcaaac cataacccat taaaaataaa
      181 taaaaaatcc ataaaatact ctgtccattc tgtaatgcaa acgggtcttt attaaataga
      241 gtagagcagc caactaatcc aattgttaaa aataatagta gatatttaat tgttgttttc
      301 gataatttat ttaaaaatcc aactgtataa ggaatactac aaaaaagcaa agtacaagaa
      361 acagcataag taaagtaatt ctgactgata ggtgttagga taagccaata atcacgaata
      421 ttcaagctgc cagcttgtac gtaaaaaaag atattgcaaa ctagtgtggc tattaggaat
      481 ataaaggcaa aacgacttaa ataatgtaaa acttttaaac gggcgatttt ttgatattgg
      541 taacccatgt aaagtgggat caaatttgca ccaataacaa cggaagtttg taaaaatgta
      601 tagatgtaga atcgaccagg gtttgttgca tatgtaggaa tagtgactgc tgaaatcaat
      661 atacgctgta aaaccatcat tatacaagcg acaaagattg tcaggaagcc ataactctcc
      721 ttctttattt ttttcaagaa taaacacctc acaaaaaaat tggacaaaaa cataagaaaa
      781 cgtttcctga taagttgccc attaataaca tcctaactta gaatatctca aaaaacaacg
      841 tgtagacaga aaaagtacaa aattagcaag aaatggttgt ttgtattgtt agaattttta
      901 gaaaattata cctttagttt ctttgcataa aatgatacaa tgattgttaa aattgtgaaa
      961 gggtggtcat ttgttagaga agtttttgga tatgtcgtta gttcaacgta ttgttgttgg
     1021 tattttagtc ggaatattgt ttggtatctt tttccccaca tggacattta tttcaatatt
     1081 gggaacgttg tttgtcagtt gcttgaaggc aatcgcacct attctggttt ttttcttgac
     1141 gatggcgtca attgccaagc ataaaattgg gaataagaca ttcgtgaagc ctattttgat
     1201 cttatattta gtgggaacat tgctttctgc acttgtagca gttattgtaa gttcaatttt
     1261 tactgttcct attactttac aagaaacagt atctgaaaaa gcaccgcaaa gtttggaaag
     1321 tgttttatct actatgttga cgaatgtcac acaaaatcct gttcaatctc ttattgatgc
     1381 gaattacctt ggcgtcttat tctgggctgt attgttggga ctagcttttc gagcacgttc
     1441 agaatcgaca aaagagttgg tggatcagat atctatagcg ttgtcacaag tcgtacaagt
     1501 gattattgca tttgctccga tagggatttt aggattggtt tatcaatcaa ttgctacgac
     1561 tggaatcgct ggattggcag agtatctgca gcttttactt gttttgatcg gtacgatgct
     1621 gtttgtcgca ttagttgtgt acccattcct cacgtttttg tttattaaag aaaatcccta
     1681 tccattgatt ttcttttgtt tgaaagaaag cgcacttccc gctttcttta cccgaagttc
     1741 tgccgcaaat atcccgataa acatgatgtt ggctgaaaga ttgaaattga caaaagagtc
     1801 ttattccata tcgatcccgt taggtgctac gatcaatatg ggcggtgccg cgattaccat
     1861 ttccttgatg acgttgactg ctgtacattc tttagggatt gaagcacctt ttgttttgaa
     1921 agtcctttta tgtattttgt ctgctttagc tgcttgtgga gcttcaggtg ttgcgggcgg
     1981 ttcactgtta ttgattccat tagcatgtag tttgtttggt atctctaatg atgtcgcgat
     2041 gcaaatcgtc ggtattggat ttattatcgg tgttatccag gattcaatgg aaactgcctt
     2101 aaattcgtca agtgatctgt tgtttactgc aataggagag ctaggcgcgc gtaaaagaag
     2161 tggggaaaaa atcaaattga aagattgttt agatggatta agtgaacgtt gaacgttaga
     2221 tatttagtat ttttaaaatg attcgagttt ttaatgagtt atatagatgt tcgtgtaaat
     2281 agaggtttct gttaaatcaa gataaagtcg tttataataa caattaaaag cataggtaat
     2341 tgttattgga ggcgtatcat ggatttttta cgtaaatttg gttttttctt tttattatta
     2401 ggcgtgctga gtgatttttt gactccgtac gtattaggat tgtattatcc tgaattagat
     2461 caaatgaaga gcgtgatcag tgtatttgga gaggtttcca gtcctgtaag aaaagcgttt
     2521 cttatttggt ctgtggtttc agggatcttt ttcatcctgt cattgcctgc attgtatgct
     2581 gcatttgtta gtacatctcg tgtgttagct accctagtta cagcagccat cgcgttctat
     2641 ggtataggtg attgtatctt tacaggtttg ttcagtatca atacccatga atcttcgtgg
     2701 aatgtctcga catgggtcca taatatcgga tcaggtttag gttacagtgg ttttttattg
     2761 tttccagttc ttcttttctt gatctatcga aaaaatgggc aaatggacgt aagccactta
     2821 taccttggac tgtctgtgat cagtttgctt tttgcggcga tttatggact ggcaagaatt
     2881 ccttctataa attcttggcc attactgaat aaaataggtt tttgccaaag aatcagtttt
     2941 ttctttaatt atgtgcccat tgccaggttg agtatcttgc atctcaaaca gtaaaacttg
     3001 agccgtcaaa ttgaacagtc agcggatact tcatccggtg gctgtttttt gtttttctga
     3061 aatatatatt tccacagttt tcttgtatag cttctgtctt agagaagcaa ggagctatat
     3121 tttgtgctac aatcttaaaa aggtagagag aagaccaaat ggaagacaga ggagccacta
     3181 aaatatgaga aacatcaagt tgacaatcga atatgacgga aaaagatatt caggatggca
     3241 aagacttgga gatgacgata aaaccattca aggaaaaatc gaaaaagtat tgcatcagat
     3301 gacaaatgaa gagatagaaa ttattggatc aggaagaaca gatgctggca cccatgcgcg
     3361 aggacaagta gcaaatttta aaacaaacac agaaattgga ctggcagaaa tgatcgactt
     3421 tatgaatcgt taccttccgc gggatattgt catcaaaaga atcgaagaaa tgcctgagcg
     3481 cttccattca agatataatg cggtgggcaa aaaatacagc tattatgttt ggaacaacgt
     3541 gattccttca gcatttgagc gaaaccacag cttttatttc cctcaagagt tggatatgga
     3601 caagctgaat gcagcttgcg aaaaattagt gggtacacat gatttcattg gtttttctgc
     3661 attgaaaaaa tcaaaaaaat ccacagtgcg tacgattgat gaattatcta tcgaacgaaa
     3721 gggagaaatg cttcatttta cttttgtcgg agacggattc ttatataaaa tggtgcggat
     3781 tatcatgggg acattactag aaatcggcac aggtgccatg ccgatagaaa caatcgatga
     3841 aatatttgaa agcaaagtgc ggcaacatgc aggagaaacc gtccctgcac aaggtctgtt
     3901 tttggatgaa gtttattatc gataaaaata ctttgataat ggatgaaacg catgtttcac
     3961 agtataaata tcgatgttaa ataatgtatg aagcaaatta aagaaatata aaaaaagact
     4021 ttttagcctg tttatgtgta cagctaaaaa gtctttctta aataaaaatc tagtgagaat
     4081 gttgtacttc tcttctcggt tgtttttgtt tagaaaaagt aaatcgataa agtaaaaata
     4141 aaatcaaagt aacgatagat aaatatcttg aagcaattgc tagttttgtg tactgataag
     4201 taataacgac tttatttata cctggaggaa tagttaattc agttgtgcca aacttagata
     4261 attttgtttg tactagtttg ccattaatag aagccacttg ccctttataa cgataaatag
     4321 gtgtcgtcaa gacagtattt ttattattcg aattatctaa ttcaattgta agtttgttac
     4381 tcataaaatg agttgttgga tttatttcct ggttacctag taaatattga tcattattaa
     4441 tcatatcagg atgatagatt gactctttgt ttgcataatc agtatgattg taattttcag
     4501 ctatagcaac agcgtcttca ggtgctataa gtgttaatga atctttgtta gcatggatag
     4561 aattgcttac tcctgagaga tgcaatatta gtgcaagaaa cgtaaagacc attattttcc
     4621 atgaattatt tttagtagaa aaaatataat agatcactat actcatcgta taggctataa
     4681 ataaagaaga aaaaccattt aaccgccaga caaattgtag actcttaact ggtgtatggt
     4741 taaaaagttg ccaaggaaat aaattggttg aacaaaaaag aacaaataca ccaagaaaga
     4801 aaataaataa atcatcagca gttaattttt ttataaaaat aaaagcacca atcaatccga
     4861 gaaataagaa taatccaata gtatagttgt tatagttatt atttaaaata tgtgtcagca
     4921 tatcactagg agccatacca cttaattctt ttcccagtgg gactttcaat tcttgtgctc
     4981 tagattgttg aataaaagga attaagaatc ccgcacttaa aaaaaaggtg aggacggtag
     5041 cttttaaaag acttaagagt ctagcgattt tttggtccca aaaataaaaa gaaagaaaca
     5101 acgttcctcc aataaataca gaaaccatag cgacagaaag taaatgagta tacactacta
     5161 gcgtcatgcc aatagacaac cagtaccatt tttggtaatc tctaatataa atttcgtaac
     5221 accccattag tatcaaaggc aaaaatgtta aagcgacagc ttctcccaat gaagcacgat
     5281 ggaatatgtc aatggcacga taggcggaaa atgtataaat aatcgaaaat aacagcgaaa
     5341 tataacgatt gttttttatt tgtttcatag aaaaataaga aacgaccatt gttaaaaaag
     5401 t
//
LOCUS       DAZAOG010000164         5220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000164 DAZAOG010000000
VERSION     DAZAOG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.166"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACHDLD_002470"
     CDS             complement(<1..1256)
                     /locus_tag="ACHDLD_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN3364138.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACHDLD_002471"
     CDS             complement(1270..2487)
                     /locus_tag="ACHDLD_002471"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3364139.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACHDLD_002472"
     CDS             complement(2477..4072)
                     /locus_tag="ACHDLD_002472"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN3364140.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACHDLD_002473"
     CDS             4197..4982
                     /locus_tag="ACHDLD_002473"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFN3364141.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACHDLD_002474"
     CDS             4975..5217
                     /locus_tag="ACHDLD_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364142.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAZAOG010000165         5199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000165 DAZAOG010000000
VERSION     DAZAOG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.167"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..609
                     /locus_tag="ACHDLD_002475"
     CDS             187..609
                     /locus_tag="ACHDLD_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364143.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(775..1968)
                     /locus_tag="ACHDLD_002476"
     CDS             complement(775..1968)
                     /locus_tag="ACHDLD_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3364144.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2192..2569
                     /locus_tag="ACHDLD_002477"
     CDS             2192..2569
                     /locus_tag="ACHDLD_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3364145.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2557..2895
                     /locus_tag="ACHDLD_002478"
                     /pseudo
     CDS             2557..2895
                     /locus_tag="ACHDLD_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3029..3472
                     /locus_tag="ACHDLD_002479"
     CDS             3029..3472
                     /locus_tag="ACHDLD_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFN3364146.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3733..4548
                     /locus_tag="ACHDLD_002480"
     CDS             3733..4548
                     /locus_tag="ACHDLD_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3364147.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4558..>5199
                     /locus_tag="ACHDLD_002481"
     CDS             4558..>5199
                     /locus_tag="ACHDLD_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3364148.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKL"
BASE COUNT         1745 a          889 c          983 g         1582 t
ORIGIN      
        1 ttattttatt attagattat tgacatttat ataataaacg attaaatgat tcatatcaat
       61 agaatactta ggaaaaaaag agaaataata acaggtttat gcactattga cggtgtgaca
      121 atatgatatt tgtatccgtt tttttcttgt atgtagtata gtaaagtaga tacaggagga
      181 gaaaatatgc agactttttt aacaaaagtt tctcaaaaga aaattaaaat gattcattta
      241 ctgcttgaaa cagaacgttg gtgttcaata gaagaactac aaaacgaatt gaaggtatca
      301 agtaagtcta tcttgaatta tctaacagat ttagaagaat tgttccaaca atatcctgat
      361 aaggtcattt tgaagaatga gaataatcgg cgcttttcta tggaaaagca ggagaacttc
      421 ccaatctaca ttatttatct tcatttctac agaaaatcat ataattatca tttgattgaa
      481 ttcatgtatc aacatcctga gaaaaatttg gaagattatg cagacgctca atatacgagt
      541 gtatccacag tttttcgtta tgctaagtta ttagtaaagt actttgaaat gacggacgtc
      601 aaagaatagt cgccgccaga tagaaggaaa ttagcagttg aaaaagctgc cctgcatcaa
      661 gtaatacgac cagccaaacg gaaacagtgt ctcatgtttt tgatttggct ggtcttatta
      721 ccgcagatgc aagctttttc cacacctctt tcttgtcatt tacagactca actatcaatg
      781 ctgcaagctt tcccttaatg ctgtaacacc agtaagagtt gtcaagacca gcagactgac
      841 taggtaaata ccgctcaaaa acaagattac ggcagttaaa ctataatgat ccatcaagaa
      901 accgatgacg atagaagaaa aaccaccaac agctcgtcca aggttcataa cgaaattatt
      961 cgccgtcgcg cggatttctg ttggatacaa actactaatc actgcgccat atccaccata
     1021 catgccattg ataaagtagc cgactatcac agcggctgct accaaactcc actggctatg
     1081 ggctaacaca agtagataga cagataagga tgaacaaatt aaaaacaaag aaaaggctag
     1141 tttaggcccc aatttatcca tgattatgcc aaaagtcatc atccccaaag acatgcctaa
     1201 aatagtactg accatccaca atgaagaccc agaaaccgaa agccctaatc gattttgcat
     1261 aattgaaggt agccaattca ttaacccaaa atagccggca atttggacag ttaccatgat
     1321 actaaggcca catgtttgcc aagcaagatg ttttgattca aacagtctgc taagtttggt
     1381 tttcttttcg gcagattgta cagatttgaa ttcctttggt tcagtcaagc cgcgacgaat
     1441 gaacaagacc atcaacaccg gcagtacacc tacgacatac aacatcttcc aaccaaacgt
     1501 tggaatgatc acagcagcta aaatcgcagc caaagccgca ccgacctgcg caccaatacc
     1561 agcaaccgaa gaagctcgtc caatttgttt ttttgaaaaa ctttcggaaa tcaaggacat
     1621 acacgcccca tattctcctc ccgctcctac acctgcgata aagcgaaaga gataaaccaa
     1681 gtagatgttt gaagcaaaat acatcaataa agaagcaatc gaaaacagaa ggaccgtttg
     1741 agaaaatact ttcacacgtc catatttatc agctatgatg ccaaaaaaaa tacctccaat
     1801 gagcatcccc aaattagtga tcgttgaaat gaaacccgct tgcgtaccac taatatgaaa
     1861 agaagtaatc attgaagata atgaaaaagc taaaaacata atatccatat tctctaaagc
     1921 aattcccgcc gatgtcgata ctaatacctt tcgctgatac gttgtcattt ttatacccct
     1981 tttcctaaaa tttccctaaa ttgttaaaag tatatccaaa aaagaataaa aaaacaagac
     2041 ttttttattt gaaataatac acaaacaacc gatatagata atccagctta gttttaatag
     2101 aaatgagtat tgacttggag tacacttaaa gttgtagaat aaataaaata cataagtaaa
     2161 gtggcaagaa attaagagaa agagtgatca gatgaatatc aaacaagtca gtgaagaaaa
     2221 gggcatttcg gccgatacat tgcgctatta tgaacgaatc gggttgattc caccagttaa
     2281 tcggacaaaa ggcggaatcc gtgattatac agaagaagac ctaaaatggg tcgactttac
     2341 gatctgtatg cgaaatgccg gattatcgat cgagtctttg attgaatata ttcgcctatc
     2401 tagcgaagga gaagcaactg tatttgagcg gcgtaagctt ttgattgaag aaagtgaact
     2461 tttgacgaaa aaaattgctg aaatgcaaga atgtttggaa cgtttacaag gaaaaatcaa
     2521 gaaatatgat cgtgtgttat ctagaaataa aatggaatgt atcaagtaaa aaaatcaagg
     2581 agcatcgcta tggagttacg cttactaaaa tatttttgga caattgcaga agaaggaaca
     2641 atctctcaag ccgcagaagt tctacatctt acacaaccaa ccttaagccg acaactgaag
     2701 gagttagaag agtaactagg aacagaactg tttgtacgcg aaaaccgcaa aatgatcttg
     2761 acagaagcag gttattttct aaaaaagccg agcagaagaa attttagatt taacgaatca
     2821 aaccactcaa gaattcgaga acagaaaaaa acaattattc agcggccgat ttacgatcgg
     2881 ctgtgtggaa gctgatagtt cgaatacatt ggcactatta ctagaaaagt tgatccgtga
     2941 ttatcccagc gttcaatttc atatccacag cggaacaagt gatgatatca cggagtgact
     3001 agaaaaaagc ctgttagatt tagcgatttt gctagatcca gtagatacag agaaatacga
     3061 aacgattttg ttaccaagaa aggaaaaatg gggattgctc attttagaaa attctgtttt
     3121 agcaaaaaaa gacgtgataa caaaagaaga ctttgcgaat gtacctcttt tgatctcaaa
     3181 gcgtccagaa atacagcgaa tgtttgctga ctggttaggc ataaaaaagg agcagttgct
     3241 gatcacagga acgtacaatt tgattttcaa tgtatttgcg ctaattgaga atcaggtagg
     3301 tgctgcttta gtcatagaag gtgctgtaag tcatcattca aagcgagtga cattcgttcc
     3361 gcttgaccca atgattgaaa cgaactgcat gctagtctgg aaaaagaatc ggattttatc
     3421 acctgtagtc aatgaattga ttcaccgttt caaagaagct tttgaacagt aataagtatc
     3481 tctaatcgaa gaaatttcaa tacaaaagcg agacgtatga cagtcttgct tttgtatttt
     3541 ttcattttta aatcaaaaaa ataaagagat ttacataact tgttaaataa gcgcttacaa
     3601 aaataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt gtaatcatta
     3661 caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa agcaaaaatt
     3721 cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat tgtattagct
     3781 ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc aaataatgaa
     3841 gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa ttatctggtt
     3901 atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt aagcgttgtg
     3961 atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc taaactaaat
     4021 tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt cccttggcag
     4081 tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacaga tacgttgtgg
     4141 tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa gcaatattac
     4201 agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc agagtgggga
     4261 atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt gacgatcatc
     4321 acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc ttctacggaa
     4381 aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc agagtacgca
     4441 ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt tttagcgatg
     4501 caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga ggtgattatg
     4561 aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt gtatcaatca
     4621 ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc tgcgttcctt
     4681 ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt tattggtcca
     4741 ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaatag tttttcacca
     4801 gtacgtttgt tcataaagat gtttcgccaa ttttggctaa agggattttt ttattggcta
     4861 tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat acgtttttct
     4921 tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt ttcctttagc
     4981 accaactgct ggtattttca ggtgagaaat ccagcgtcga aaccaaatca agttctacgg
     5041 atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt tttactattg
     5101 acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact aggtccttct
     5161 atcgtatttg gtttgattta cttcaacaat cgtaaattg
//
LOCUS       DAZAOG010000166         5156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000166 DAZAOG010000000
VERSION     DAZAOG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.168"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..720)
                     /locus_tag="ACHDLD_002482"
     CDS             complement(58..720)
                     /locus_tag="ACHDLD_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3364149.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            904..2550
                     /locus_tag="ACHDLD_002483"
     CDS             904..2550
                     /locus_tag="ACHDLD_002483"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN3364150.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2541..3623
                     /gene="uxuA"
                     /locus_tag="ACHDLD_002484"
     CDS             2541..3623
                     /gene="uxuA"
                     /locus_tag="ACHDLD_002484"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3364151.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3731..5156)
                     /locus_tag="ACHDLD_002485"
                     /pseudo
     CDS             complement(3731..5156)
                     /locus_tag="ACHDLD_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1665 a          887 c          986 g         1618 t
ORIGIN      
        1 aaagattact caaaaacgaa ggttaattat aaaaatagaa gaggtcgcaa gaaaatgcta
       61 ctgctggaaa tactcgggat aacgttgagt aacagtttcc tgttctttca gcatcatgtg
      121 taagtgcaat cccgtttgaa atcgagcttc ttctggtttt ttatcaacta aagccttgag
      181 taaatcataa tgttgttcca tgatcgtttc ccatggaagc ccctgaatgg tcactcgtaa
      241 ccagcggaaa cgttctaaat gggtattatg gtcatctaac cagttccaaa cttcttttct
      301 tcctgcaatt tcgaaacaag cttcatgaaa taaattatct gcacggaaaa aagcgcgttc
      361 gtcatttatt ttgactgctt tttcttgttc ttctaaaatc gtttctagca ctattcgtgt
      421 ttgtgtgtcc aatttggaac aacattcaat catgatttgc tcttccaact gttctcggac
      481 aaagcgagca ttttctgcag aaacaagatt aattttggaa acatacgtac cactttgagg
      541 tatcgtatac acgagttctt gctgacgtag ttgaatcaat gcttcgcgaa tgggagtccg
      601 gccgatagca agatgttcct ccagtgcctt ttcagatatt ttccttccag gttctaagtc
      661 agcatagata atttgtttac gaatcgcttg ataagcttgt gattgcaagc tgcttttcat
      721 ctttattcct ccaaaatcat ttctatgaac gtagtataac agaagaggtc tgaaaaacaa
      781 tctggtatgt cacattaact agtaaatttg tataaatagt aaaattttgg ttgacatagc
      841 gatttttatt gcataaaatt cacttgaata caaaaactaa tatgttagtt taggagggga
      901 aaaatgattg atttaaacaa ctggaggaac tttatccctg aattatctca aaaagaagta
      961 gaaatacctc attttgaccg tgcatcatta agagaaaatg gacaaaaaaa tcctgtctgg
     1021 atccatttcg gtggcggaaa tctttatcga ggatttcatg cagaaatcgc tcaacgttta
     1081 gcggatctag gcgaattgtc cactggtgtc attgtttgtg aaacattcga tgaagaaata
     1141 attgaaaggg cttaccaaga ttttgatgat ttgattcttg aagtggtcat gccaagcagt
     1201 ggagagttcg tcaaacgtat attagctgcg acagctgaca gtctgtattg tcatccagat
     1261 tgttctgaac actttgataa gatgattcga cactttgaaa atcctagttt acaattagtt
     1321 acttttgcta ttactgaaaa aggttatcga ttgaaagata caaataataa atatatagga
     1381 ccgattgcag tcgatataaa aaatggtcca aagaagccat cacatacaat gagtattctt
     1441 gctgtgctac tttataaacg ttttttatct ggagcttggc cgattgcact tgtctctaca
     1501 gataattttt cacaaaacgg tagtcaatta cgtacttctt tgctaacgat cgcaaaagaa
     1561 tggcaaaagg aaggttatgt acaaaataca tttatccaat acttatcgaa tgaagaaatt
     1621 gttgcgtttc catggacgat gattgatcgt atcaccccga atccttcctt gtcagttagc
     1681 aaaatgttgg caaaagaggg cttttcaaat atgtcgattt tccaaaccgc aaaaggaaat
     1741 aatattgctc catttgtcaa tacagaggac atccattatt taatcatgga agatcatttt
     1801 cccaatggac gtccgaaatt cgaaaaagct ggggtattct tgactgatcg tgcaacggta
     1861 gataaagcag atacgatgaa ggtgacgact tgccttaatc ctttgcatac gtctttagct
     1921 attatgggtt gtttactggg gtataggagc atagctgagg agatgaaaga tccagattta
     1981 tcgctgctag tttctgaaat cgggtacaat gaaggcctcc cagttgtgga gtctccaggt
     2041 atcattgatc caaagatctt tctggaagaa gtaattactg tgcgcttttc taatccaaat
     2101 attcctaata tgcctcagcg aatcgccacg gatacctctc aaaaggtggc gattcgttat
     2161 ggtgagacga ttaaaaaata tatggaaata gaagttaaag acccgcagac acttgtattc
     2221 ataccattga ctctcgctgc ttggcttcga taccttattg gcattgatga tcaaggaaaa
     2281 ccattcatcc cgagtccaga cccactattg acagaccttc agcataagct ttccgatatt
     2341 caactgggta cgcaagatac atcacatatt catgagaaaa tggctcctat tttgtcaaat
     2401 aaagaaattt ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat agaagaaatg
     2461 ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa cattacagga ttacttacaa
     2521 gaacatggag gaaatttatc atggaaatga cttttagatg gtacggatca caggaagaaa
     2581 agattacctt agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga
     2641 tggatatacc agtaggagag gtatggccta gggagaggat caaagaacta aaagaagaaa
     2701 ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta
     2761 aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact
     2821 tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga
     2881 aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg
     2941 atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg
     3001 gattgccggg atgggaacct gagcgcttag ctcctatcaa atccttgctt gaagcctatg
     3061 catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc
     3121 caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga
     3181 tttttggatt agctcgaatc gtaaagaatc gagaagattt agattggttg tgtcgcatag
     3241 tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa
     3301 atgatgtata tgggatttta gcagaatttt gccaaagaga tcgaatccca tttgcacatg
     3361 ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccacat aaatccgaat
     3421 acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacggat
     3481 acatccgtcc tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct
     3541 tatatgatcg agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag
     3601 gaaacaaaag taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg
     3661 atgttcaaat tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat
     3721 cttatttggt ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt
     3781 cttttaattc aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg
     3841 tgatgattcc ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc
     3901 agtcgctgat aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat
     3961 ttgaaaaaat ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat
     4021 agttcacacc aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt
     4081 cttcaaccat ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt
     4141 gttctctgaa atccatataa aattgttcaa tggcggtttg gacttctttc aatacgctat
     4201 gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc
     4261 cattataaag tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta
     4321 taaacaaatc aaatacagta caaagggact ccaccatttc tcttgaataa agaacatgag
     4381 gaacgtttgc tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt
     4441 tcgtgataga aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca
     4501 gattaggtcg caaaatatct acagagagat caataaaaaa gtcaatatat tcatactctg
     4561 tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta
     4621 atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa
     4681 aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag
     4741 tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc
     4801 cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa
     4861 atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac
     4921 tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga
     4981 ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt
     5041 caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac
     5101 gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca gacata
//
LOCUS       DAZAOG010000167         5061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000167 DAZAOG010000000
VERSION     DAZAOG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.169"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACHDLD_002486"
     CDS             complement(205..660)
                     /locus_tag="ACHDLD_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFN3364152.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACHDLD_002487"
     CDS             complement(1063..1236)
                     /locus_tag="ACHDLD_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364153.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACHDLD_002488"
     CDS             complement(1917..2456)
                     /locus_tag="ACHDLD_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3364154.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACHDLD_002489"
     CDS             complement(2472..3395)
                     /locus_tag="ACHDLD_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3364155.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACHDLD_002490"
     CDS             complement(4523..4678)
                     /locus_tag="ACHDLD_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364156.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACHDLD_002491"
     CDS             complement(4697..>5061)
                     /locus_tag="ACHDLD_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFN3364157.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAZAOG010000168         4903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000168 DAZAOG010000000
VERSION     DAZAOG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.170"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1212
                     /gene="truB"
                     /locus_tag="ACHDLD_002492"
     CDS             289..1212
                     /gene="truB"
                     /locus_tag="ACHDLD_002492"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN3364158.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1216..2157
                     /gene="ribF"
                     /locus_tag="ACHDLD_002493"
     CDS             1216..2157
                     /gene="ribF"
                     /locus_tag="ACHDLD_002493"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN3364159.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2509..3294
                     /locus_tag="ACHDLD_002494"
     CDS             2509..3294
                     /locus_tag="ACHDLD_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3364160.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3307..4158
                     /locus_tag="ACHDLD_002495"
     CDS             3307..4158
                     /locus_tag="ACHDLD_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN3364161.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4282..4734)
                     /locus_tag="ACHDLD_002496"
     CDS             complement(4282..4734)
                     /locus_tag="ACHDLD_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364162.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         1624 a          803 c         1050 g         1426 t
ORIGIN      
        1 aagactggaa gaatggccag tctttttttt cgtttttggg aagtgaaaac caaactattt
       61 acctgtaagc cacccataaa tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt
      121 ccctccttta aaaaagcata acatgatttc tatttacgtg agcatgaatt ttgttacgtt
      181 tttattgccg aaaaataaaa accgttcatt atgatgtcaa attcgtcagt aattgatgca
      241 atgtgttata ataattcagt taaatcaaaa atgaaatgga gaaaagacat ggacgggtta
      301 ttacctttat ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa attaagaaaa
      361 atcttacata cgaaaaaaat cggacatggg ggtacgcttg acccagacgt agacggtgtg
      421 ttgcccattt gtattggaaa agccacaaaa gttatcgaat atttgacaga ttctgggaaa
      481 atctatgtag gcgagataac tctaggtttc agtacaacga cggaagatcg atccggagaa
      541 cgggtagaaa caaaaccagt agatatgtct ttatctgaaa aaaaaataga tgaagtgatg
      601 gcttcattta ttggtgagat cacccagata ccaccaatgt actcagcggt aaaagtcaac
      661 ggtaagcggt tatatgaata tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag
      721 gctcagattt accgttttat ccggacaagt gaattgacaa gagacgaagt gacaggaacc
      781 atttcttggc gcttcgaagt agaatgcggg aaaggaacct atgtccgcac attagctgta
      841 gatacaggca agaaacttgg ttaccctgcg cacatgtctg atctgacccg aacagcaagt
      901 gccggtatgt cccagaagga tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat
      961 gggacaatcg atgattgttt gctgccaata gaaaaaggag tggaacggtt caaacgagta
     1021 gatattactg atgaagtatg gcagaaagta aaaaacggta tgagacttga ttatactgta
     1081 tttgcgctcc cagaaatgcc gtcagaagaa attgctttgt tttataaagg aaaagttgtc
     1141 agtatttatc agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa agtattgaga
     1201 aatgaggaat aacgaatgaa tatcatcaag atcagacatc cttaccaatc agaacaaatt
     1261 ccagaagacg atgtcgtttt agtgctggga tttttcgacg gcgtgcatat cggacaccag
     1321 aaagtcataa agacaggtat ggaaattgca caaaaagaag gattgaaatt agcattgatg
     1381 actttcaatc agcatccatc tattgtcttt aaaaagatcg atcctagcag cgtgaaatat
     1441 ttgactagcc ttaagcaaaa agaagaaaaa atggccgctt tgggtataga ttatttatat
     1501 gaagttgatt ttacttcttc ttttgctcat ttagcaccgc aggaatttgt tgatcaatac
     1561 atcgtagggt tacatgcaaa atatgctgtt tccggttttg attatacgta tggaccaaaa
     1621 gacattgcag atgtcgctca ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca
     1681 gtttctaaag aagaagaaca aggtgaaaaa atcagctcta cccgtattcg tggattacta
     1741 gacagcggac aagtaaaaga agcagcaaaa ctattaggtt gcacatatga ggtagaaggg
     1801 gtagttgtcc atggcgatgc acgaggcaga ctactaggat tccctacagc aaatgtcaaa
     1861 gtaaagagca cggttcattt gcctaaagaa ggtgtttatg tgtcggaaat aaaaattggg
     1921 gaaacatggt atccagccat gggttcaatt ggacataatg atacattcgg tgaaggacgg
     1981 gagctgactg tagagttgta tattttggac tttaatcaag atatctatgg tgaacatgtc
     2041 gcggtacgct ggaatcaatt tttaagagat caagtgaaat tttctagtgt agaggaattg
     2101 attgaacaac tcaaggagga tgaacaagca acagctgaat atttttctca gcaataaaac
     2161 attatgcata aaatctcttg tttatgtata actagcaatt acaataaaat gggtgataaa
     2221 taagcccagg aaaggggctg tgacaaaagt cttgtcacag cctttttttc caaataaatg
     2281 gtttttcaga agtagcttct tcggaaataa gccgaaattt cttgaagctg accacttctg
     2341 tcacaacctc tttctttata gaaataaaag gacactagca attcatgaaa acgattgcta
     2401 gagcgtatca tttgctttag actatatatc ttggtgtaca ctcactttaa ggcggaaata
     2461 aaagttatcc gtaataacag gtttaggtat gaaaaaggag tgggtactat gtacaaggaa
     2521 ttagaaaaca aagtcgcagt agtaactggt ggctcaaagg ggattggaac agcgattgct
     2581 caacgattcg ggaaagaaaa aatgaatgta gttgtcaact ataactcgga taaagaagga
     2641 gcagaaaaag cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc tgtacaagca
     2701 gatgtcagtt ctgaagaagg aatacagcgt ttacttgata cggctttaga aaattttggg
     2761 acactggacg tttgggttaa taatgctggg atggaaaatc agcatccaac ccatgaactt
     2821 agccttgaag attgggaaag agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg
     2881 aaagcggctt taaattactt tacagagaat aataaaaaag gaaatatcat caatatgtct
     2941 tctgttcatg aacagatccc atggccaacg tttgctcatt acgctgcttc aaaaggcggg
     3001 gtaaaattat ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat cagaatcaat
     3061 agtataggcc ctggtgctat ccgtacacct attaatgcgg caaaatttga tgatcctgaa
     3121 caaaaagaac tcttggaaag tatgatccca ctaggacgcg taggtgaacc aaaagaaata
     3181 gcggcgacag ctgcatggtt ggcttcagac gaatccagtt atgttacagg tattacacta
     3241 tttgcagatg gtgggatgac tttatatcct tctttccaag gtggaaaagg ataagggtga
     3301 ataaaaatgg gtttattgat tgcattgatc ccggcagttg gctgggggat ccagccactg
     3361 attttgaaaa agattggtgg cagaccaaca aatgagatat taggaactgg tattggagcg
     3421 gtgcttgtcg gcttactcgt tcaattattc gcttctccag ctggtatttc agtgactaca
     3481 tttttgatta gtttactttc tggtgcattt tgggttattg gacaaacagg acagtataca
     3541 tctttcaact tgattggtgt ttctaaaacg atgccaattt cgactgcatt acagttagtc
     3601 ggaacctcgt tgattggtgt cttcgctttt ggagaatggt ctggaagtat ggggaaaatc
     3661 attggagcag cagcaatcat tcttcttgta tttggttctg ctttaacggc aatcagtgat
     3721 gatcctggaa aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc aacaagtatt
     3781 ggttattggg tatacagtgc attgcctaaa ctagttgatg caagcggact ttccattttc
     3841 tttccgcaaa tgatcggtgt ctttttaggg gcagtgattt atgtgatttt ccgtaaacca
     3901 accgcatttt ctgaagacaa gagttggaaa gcaacgatcg ttggagccat ctttagtgtg
     3961 tcagcattgg cttatatttt ttcagcaaat gaaaatgggg ttgctactgc atatatcatt
     4021 acacagctaa acgtagttat ctctacatta ggcggtatgt acatcctgca tgaaaataag
     4081 acaccaaaag aaaaaaaatt aacgattatc ggattattgc tgatcgtagt aggaagcatg
     4141 gtcacggtct ttatttgagg ataaagatgg taaaatttca tttattaaaa aaatatttgt
     4201 ttgaaccaaa cagtaagtac ttataaaaaa atagcgctag aatttccaac atctctggaa
     4261 tttctagcgc tttttaattg tttaagatac aagggaaata tctgtctgga tcaataattt
     4321 ttcattgatc cattgactaa agtttacgac ataattgaag ttaccaccga ttagtatctc
     4381 cgcatgattg tctttataaa gatcgtattg taaaagacgg tcttttttat ttgcgtaaac
     4441 atcgttatgg tactggacta attgttctcg gaattgattg attgctgtaa aatcacgata
     4501 ttgaaggttg aaacgcatcg tgattggtgc atttagcatg aatcgctgca tttcttttgc
     4561 tgcttcctct gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag aaaatttcca
     4621 ttgcccgcca acctttgttc cttttagttt tccttcatga agataatttc tgatagaacg
     4681 tgtagtaaca cctaattgtt cggctaattc atttactgag taaaaatctt tcaatgttca
     4741 ccctcctatg tgaatggttc tactgactta gttgatattt gcttaatcac gaaatgtaga
     4801 aagaacgatt ttaattaagg tcattgtaaa aatcaagtca gaaaattcta acttttattt
     4861 ctctaaagaa attcgttctc tcctaaagtg atattatatt taa
//
LOCUS       DAZAOG010000169         4808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000169 DAZAOG010000000
VERSION     DAZAOG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.171"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACHDLD_002497"
     CDS             72..1118
                     /locus_tag="ACHDLD_002497"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3364163.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACHDLD_002498"
     CDS             1135..2358
                     /locus_tag="ACHDLD_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3364164.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACHDLD_002499"
     CDS             2447..3334
                     /locus_tag="ACHDLD_002499"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3364165.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACHDLD_002500"
     CDS             3420..3650
                     /locus_tag="ACHDLD_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN3364166.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACHDLD_002501"
     CDS             complement(3707..4672)
                     /locus_tag="ACHDLD_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3364167.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1628 a          795 c          975 g         1410 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga gatagatgat
     4801 ttttctaa
//
LOCUS       DAZAOG010000170         4790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000170 DAZAOG010000000
VERSION     DAZAOG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.172"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1704)
                     /locus_tag="ACHDLD_002502"
     CDS             complement(94..1704)
                     /locus_tag="ACHDLD_002502"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN3364168.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1926..2171)
                     /locus_tag="ACHDLD_002503"
     CDS             complement(1926..2171)
                     /locus_tag="ACHDLD_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364169.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2897..4237)
                     /gene="glnA"
                     /locus_tag="ACHDLD_002504"
     CDS             complement(2897..4237)
                     /gene="glnA"
                     /locus_tag="ACHDLD_002504"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN3364170.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4265..4657)
                     /locus_tag="ACHDLD_002505"
     CDS             complement(4265..4657)
                     /locus_tag="ACHDLD_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3364171.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1420 a         1038 c          788 g         1544 t
ORIGIN      
        1 acaaataata acaaatcaga aaaagagaaa agtgacaaga aaattgctta tccccttctc
       61 ttgctacttt tctctttcta tcaagttcaa aaattatttt gtttcgtgat ctgctaatga
      121 tgctccgaca aatgctttga ttaatttttg cgggcgattt ggacgagaaa tcaattctgg
      181 gtggaattgg catcccacaa agaatttttt ctctggcagc tctacgattt ctactaaacg
      241 gttatctggt gagacacctg aaaagactag cccttgttct tcaaacaatt gacgatattt
      301 gttattgaac tcatagcggt gacggtgacg ttcttgcaca acatcggctc catcatatgc
      361 ttcagccgtc ttcgttcctt ttttgatttt acatgggtat aatccaaggc gtaaagttcc
      421 acccaaattc tcgatgtttt cctgatcagc cattaaatca ataatattgt ttgctgtatc
      481 tggattcgtt tcagctgatg acgcatcttc taatccaacg acgttacgtg caaattcgac
      541 acaagccatc tgcataccaa gacaaatacc taaaaacgga acgtcatttt cgcgagcata
      601 acgaatcgct tcgattttcc cttctactcc gcgatcacca aatccaccag gaaccaagat
      661 tccatctgcg tctttcaaac gttcttctac attttctctc gttaattctt gtgctttgat
      721 ccaatcgatc tcgatgtctg aattataggc gaaaccagaa tgtttcaatg cttcaactac
      781 tgagatatat gcatctggta actcgacata tttaccgaca agcgcaatcc gtactttttt
      841 cttcaagttc aagacacgtt cttctaattc tttccattct gtcatgtctg cttctggtgt
      901 atctagtttt aaatggtcgc aaacaatttg atccattcct tgtgcttgta aaactaatgg
      961 gatggagtaa agtgtatcta catctggcga ctcgataact gcttctggtt cgacatcaca
     1021 aaattgtgcc aatttgtttt tcacgctttg agaaacaggt tgctctgtac gtaccaccaa
     1081 aatattcggt tggataccta aaccacgtag ctcttttaca ctgtgctgcg ttggtttagt
     1141 tttcatttct ccagcagctt tcaagtaagg aatcaatgtt gtatggatat acataacatt
     1201 atctgtaccg acatctgctt tcatttgacg aagtgcttca aggaatggca aagattctat
     1261 atcccctact gttcccccta cttctgtaat gatcacatct gaatcagtca ttttagctgc
     1321 tcgcatgatt ttttctttga tctcatttgt aatatgagga ataacttgaa cagttgctcc
     1381 caagtattcg cccttacgtt ctttacgcaa aacttcagaa tagatttttc ctgtcgtcac
     1441 gttggagtat ttgttcaaat tgatgtcaat aaaacgttcg tagtggccca gatccaaatc
     1501 tgtttcagca ccatcatctg ttacgaatac ttctccgtgc tgatatgggc tcatcgtccc
     1561 cggatccacg ttgatgtatg gatcaaattt ttggatagtt acttttaagc cgcggttttt
     1621 caataagcgt cctaaagaag ctgcaacaat cccttttccg attgatgaaa cgacaccgcc
     1681 ggtcacaaaa atatattttg tcataagttc aaaaactcct ttttaattta tgtgcaggaa
     1741 aagtctcaga caataaacaa agctccctat tcttcaataa gaatagggag ccgaaatttc
     1801 tgtaaacttc tgaccctcta aaagggtgcc caaggtgtat aatacaagct gactaaggga
     1861 aagtcaagta ctcaaatgaa tactatccgc ttacaatttt ttctttttcg atatcctttg
     1921 agaaattatt tcttaagtcc tttatgagtg caaaaaaaat ttaacccaca taataagctt
     1981 gatataaaca tagctaacgt taaacctaag caaatcagaa aatcgttact gaaaaatttt
     2041 aagtgaaata atagataaaa aaataaaaca aacattatga tagataatat cccacctaac
     2101 aaagaactac tttttcctac ttgcagcaac agtttatata gcttatcgta gaaacaatct
     2161 ttttctttca cccttattta cctcctctac gtaaaaaacg tagtccacag ttctcggatt
     2221 tttggcatat cttttctatt aaataatatc aaaaacaaaa caattccact aattttttca
     2281 aagatgacct ttttttcaac aaaagtgcct tgtagggatt cattgaaggt aacgaatcac
     2341 ataagtaaaa aataaggaga tgcgacaaaa gtcgtgcctc cttatttcta aataaacggt
     2401 gttctcctgc caactcctcg gtcacaagtc acaatatcca tcaaacatga gaagctgttt
     2461 ttgaaattat ttcttgtgat tcggagttaa atggtaggct cacaacctct ggataaacgg
     2521 tattcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     2581 ttcacaaaat ttttcttaat actcgaaact aaacgtcttt ttcaaaacct cttggtaaac
     2641 ggtgttcaaa agcttgcatc tgcggtaata agttcagcca attcaaaaac aagaaaagct
     2701 gtttttgctt ggctattctt attacttgtt gcagggcagc tttttcacaa cctctccact
     2761 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     2821 ctatttttcc gttagtttct ctaatactca ggtcaaaacg ctgatttcac agcctctaat
     2881 tcacggtatt cctgttttaa taaagttcta gatattgttc tctttcccac tcggaaacag
     2941 tttgtctgaa tgccgcccat tcgagacgtt ttgcttcaac aaaatttgag aagatatgtt
     3001 gtcctaatgc gtcaatcatt acattatcct tacgcaattc tttgatagca ttatggatag
     3061 ttgaaggtaa gtcctggata tgtgcagctt ctctttcttc ctcatccatt acataaatgt
     3121 tacggtcaac agctgctggc ggtgtgatct gattacggat accgtctagt cctgcttgca
     3181 acaatactgc cattgccaag tatgggtttg cagatggatc aactgaccgc aattccaaac
     3241 gtgtggataa accgcgagat tctggtacac gtactaatgg cgaacgatta cggccactcc
     3301 atgcgacata aacaggcgct tcataacctg gaacgagacg tttataagaa ttgactgttg
     3361 gattgcaaac ggctgtgtag gcacgagcat gtttcagcaa accacctaag aaatgataag
     3421 ctgtttcact tagttccaat tcaccgtctt tatcatagaa aacatttcct tcatcattga
     3481 acaaagacat attgcaatgc ataccagaac cattgatccc aaataacggt ttcggcatga
     3541 aagtcgcgtg aagtccatgc ttgcgtgcaa tcgttttgac aacgagcttg aaggtttgga
     3601 tattatcaca agcatctacc acatctgcat atttaaagtc aatttcatgc tgtcctggcg
     3661 ctacttcatg atgtgaagct tccacttcaa agcctagact ttctaattcc aacacgatgt
     3721 cacggcggca attttcacct aaatctgttg gtgcaaaatc aaaataacca cctttatcat
     3781 tcaaggtcgt tgtgatccca ccgtcttcgt ctaatttaaa taagaagaat tctggttcag
     3841 gtcctaaatt gaaagaggta aaaccaaggt cgcgcatatc tttcaatgct cgtttcaaat
     3901 tcccgcgagg atcgccggca aatggtgtcc catccggatt atagatatca caaattaaac
     3961 gagcaacttt tccatgagca ctttcccacg ggaagatcat ccaagtggag agatctggat
     4021 acaaatacat atcgctttct tcaattcgaa caaatccttc aatcgaagaa ccgtcaaaca
     4081 tcattttatt ttctaatact ttgtcgatct ggctcactgg aacttctaca tttttgatcg
     4141 tacctaagat gtctgtgaac atcaatcgta aaaatcgtac gttttcttcc tcaatgattc
     4201 gtttgatttc ttcacctgtt atctgtttct ttaccatggt atccctcttc cttttttaat
     4261 ttattcagtc aaagaattgt ctttgcaatc cttgcgactg ataatgacct gttttgctta
     4321 acccaccttg cgccaaaatc tcatcacgaa aaatttgtcg aacgtcatga tctgtcaacg
     4381 gcttcttgtt ttttgcttct tgttttttcg cctgtgccat ttcgtataca cgtttgatcc
     4441 cagccatatt caatccctct gaaagatagt ctttaatctc aagcaacata tcaatatcat
     4501 tcagcgaata catacgtcgg tttccttcgc ttcgttccgg atgaactaaa tcttgttctt
     4561 cataatagcg aatttgccga gcagacaaat cagtcagctt cataactgta ccaattggaa
     4621 atacggacat agatcggcgc aattcctttt ctcccatcag acgtcccctt tcgttttata
     4681 agttggctaa agtgtaccaa ttaaaaaaaa tattgtcaac tgcatatgtt aggttttcta
     4741 acatgagatc tctatttttt ataaaataac aaaaaaataa gaacacaaaa
//
LOCUS       DAZAOG010000171         4788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000171 DAZAOG010000000
VERSION     DAZAOG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.173"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHDLD_002506"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHDLD_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFN3364172.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHDLD_002507"
     CDS             1019..1183
                     /locus_tag="ACHDLD_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364173.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHDLD_002508"
     CDS             2141..3118
                     /locus_tag="ACHDLD_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3364174.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHDLD_002509"
     CDS             3128..3625
                     /locus_tag="ACHDLD_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3364175.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHDLD_002510"
     CDS             4001..4282
                     /locus_tag="ACHDLD_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364176.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHDLD_002511"
     CDS             4284..4700
                     /locus_tag="ACHDLD_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364177.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZAOG010000172         4712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000172 DAZAOG010000000
VERSION     DAZAOG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.174"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACHDLD_002512"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACHDLD_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACHDLD_002513"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACHDLD_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN3364178.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACHDLD_002514"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACHDLD_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACHDLD_002515"
     CDS             1786..1869
                     /locus_tag="ACHDLD_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFN3364179.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHDLD_002516"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHDLD_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN3364180.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACHDLD_002517"
     CDS             complement(2994..3857)
                     /locus_tag="ACHDLD_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3364181.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACHDLD_002518"
     CDS             complement(3859..4131)
                     /locus_tag="ACHDLD_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3364182.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACHDLD_002519"
     CDS             complement(4148..4363)
                     /locus_tag="ACHDLD_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN3364183.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="ACHDLD_002520"
     CDS             complement(4455..>4712)
                     /locus_tag="ACHDLD_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFN3364184.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DAZAOG010000173         4697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000173 DAZAOG010000000
VERSION     DAZAOG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.175"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHDLD_002521"
     CDS             <1..649
                     /locus_tag="ACHDLD_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364185.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHDLD_002522"
     CDS             708..1148
                     /locus_tag="ACHDLD_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364186.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHDLD_002523"
     CDS             1160..1828
                     /locus_tag="ACHDLD_002523"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN3364187.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHDLD_002524"
     CDS             1892..2515
                     /locus_tag="ACHDLD_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN3364188.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHDLD_002525"
     CDS             2571..2957
                     /locus_tag="ACHDLD_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN3364189.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHDLD_002526"
     CDS             3170..4561
                     /locus_tag="ACHDLD_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN3364190.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1559 a          782 c         1000 g         1356 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttg
//
LOCUS       DAZAOG010000174         4647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000174 DAZAOG010000000
VERSION     DAZAOG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.176"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACHDLD_002527"
     CDS             complement(133..717)
                     /locus_tag="ACHDLD_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3364191.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACHDLD_002528"
     CDS             complement(928..1131)
                     /locus_tag="ACHDLD_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364192.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACHDLD_002529"
     CDS             complement(1141..1563)
                     /locus_tag="ACHDLD_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364193.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACHDLD_002530"
     CDS             1801..2415
                     /locus_tag="ACHDLD_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3364194.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACHDLD_002531"
     CDS             complement(2580..3404)
                     /locus_tag="ACHDLD_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364195.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACHDLD_002532"
     CDS             complement(3428..3583)
                     /locus_tag="ACHDLD_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364196.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACHDLD_002533"
     CDS             complement(4042..4299)
                     /locus_tag="ACHDLD_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3364197.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACHDLD_002534"
     CDS             complement(4292..4561)
                     /locus_tag="ACHDLD_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3364198.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1534 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttata
//
LOCUS       DAZAOG010000175         4646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000175 DAZAOG010000000
VERSION     DAZAOG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.177"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACHDLD_002535"
     CDS             complement(<1..220)
                     /locus_tag="ACHDLD_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364199.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRK"
     gene            788..2023
                     /locus_tag="ACHDLD_002536"
     CDS             788..2023
                     /locus_tag="ACHDLD_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3364200.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(3022..3282)
                     /locus_tag="ACHDLD_002537"
     CDS             complement(3022..3282)
                     /locus_tag="ACHDLD_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN3364201.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(3309..4610)
                     /locus_tag="ACHDLD_002538"
     CDS             complement(3309..4610)
                     /locus_tag="ACHDLD_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3364202.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         1575 a          952 c          726 g         1393 t
ORIGIN      
        1 ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct
       61 ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc
      121 tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct
      181 ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa
      241 aaaattattt ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta
      301 gaacagaata aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc
      361 tccttgattt ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga
      421 aaattgtcat ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt
      481 gtgtataatg acgacagcac acctatcaat taatataatt attttatttt tttatgttct
      541 catgtttatt tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc
      601 aaaaaattgt tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat
      661 ttagaagttg tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg
      721 aacgctgtgt tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga
      781 ggatttcatg aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg
      841 aatgattgag gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc
      901 taaaactttt gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat
      961 ccttacgaaa aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa
     1021 cccttttacc aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa
     1081 gtttgagcct cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa
     1141 taaaaagatt cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc
     1201 agagcatgcg ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg
     1261 gcactggaca aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc
     1321 tgcacaaaaa gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct
     1381 aaatctcatc acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg
     1441 aatcgttggt ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt
     1501 tatacagcga aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg
     1561 caatccgcgt atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt
     1621 tattgcacct attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat
     1681 aacagaatat ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg
     1741 cggggaaggg accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat
     1801 gcaatttgag caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct
     1861 cacaaaacca gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa
     1921 gattcgtcag caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc
     1981 aattcaagaa atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc
     2041 ttttttaata gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa
     2101 aaaaagtaaa tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac
     2161 aaaatcatcc ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc
     2221 atttattctt agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt
     2281 caaaagaaag accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct
     2341 agaagcatcg ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat
     2401 gtaggattta ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct
     2461 cgcaatccga tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca
     2521 tgtcagaatg ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga
     2581 ttgctattcc cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat
     2641 catacatatt accattcata caccatactg cagcccctac tatatggttg taagctccca
     2701 aatctatatt tgacttgatc actttggtag tagatctact aattctaaat ccattgctga
     2761 taaaatttcc attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt
     2821 atgtaaaaaa gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg
     2881 tcaaaagctt gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac
     2941 aaaaatttaa gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg
     3001 ttacaaccac ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat
     3061 cataaaattg atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt
     3121 catatgcggt acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat
     3181 ccatcgattg atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt
     3241 taataaaaag ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa
     3301 aaccaaactt agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc
     3361 tttttatcat aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc
     3421 atgatcaaga taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct
     3481 ggtaatgtcc atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa
     3541 taactgataa tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc
     3601 aacatcatag gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata
     3661 gaaaaacgac caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat
     3721 accacgatcg ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga
     3781 gcgatatagg gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc
     3841 aacatgatca gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca
     3901 aatgaccaca acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct
     3961 aaaatattgg ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg
     4021 acgcttcgaa tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa
     4081 ctacgggcaa cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta
     4141 aagaaacgat atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat
     4201 gctcccaaag aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg
     4261 tttacaggaa cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta
     4321 tctaatccat atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc
     4381 aatcccatcg tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca
     4441 ttccatccag gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat
     4501 gacaatgttg aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt
     4561 gccattggtg ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact
     4621 ccttttcaat aaaatatgaa ccattc
//
LOCUS       DAZAOG010000176         4611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000176 DAZAOG010000000
VERSION     DAZAOG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.178"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACHDLD_002539"
     CDS             complement(86..400)
                     /locus_tag="ACHDLD_002539"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3364203.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACHDLD_002540"
     CDS             complement(451..705)
                     /locus_tag="ACHDLD_002540"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364204.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHDLD_002541"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHDLD_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN3364205.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACHDLD_002542"
     CDS             complement(1145..2098)
                     /locus_tag="ACHDLD_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3364206.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACHDLD_002543"
     CDS             2710..4203
                     /locus_tag="ACHDLD_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN3364207.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="ACHDLD_002544"
     CDS             4338..>4611
                     /locus_tag="ACHDLD_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN3364208.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DAZAOG010000177         4551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000177 DAZAOG010000000
VERSION     DAZAOG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.179"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..553
                     /gene="hslV"
                     /locus_tag="ACHDLD_002545"
     CDS             5..553
                     /gene="hslV"
                     /locus_tag="ACHDLD_002545"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN3364209.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            567..1967
                     /gene="hslU"
                     /locus_tag="ACHDLD_002546"
     CDS             567..1967
                     /gene="hslU"
                     /locus_tag="ACHDLD_002546"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN3364210.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1985..2776
                     /gene="codY"
                     /locus_tag="ACHDLD_002547"
     CDS             1985..2776
                     /gene="codY"
                     /locus_tag="ACHDLD_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN3364211.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2858..3733
                     /locus_tag="ACHDLD_002548"
     CDS             2858..3733
                     /locus_tag="ACHDLD_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN3364212.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="ACHDLD_002549"
     CDS             complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="ACHDLD_002549"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN3364213.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
BASE COUNT         1569 a          809 c          984 g         1189 t
ORIGIN      
        1 aaacatggtc gaatcaccat ttcattcaac cactatctgt gccgtcgaaa aagacgggaa
       61 atttgctatg gctggagatg gccaagtaac aatgggtgaa tctgttgtca tgaaaggcac
      121 tgcaaagaaa gtacgacgta tctacaatgg agaagttgtt gtaggtttcg ccggaagtgt
      181 tgcagatgcg tttactttag aagaaaaatt tgaaggcaaa ctaaacgaat ataacgggaa
      241 tctacaacgc gcagctgtcg aattagctca agaatggcgg acacagcaat ctatgcaaaa
      301 acttgaagcg atgctgatcg taatgaatga taaagaaatg cttcttgttt caggtacagg
      361 agaagttatc acaccagatg atggtatctt agctatcgga tcaggaggga attttgcgtt
      421 ggctgcagcc cgagcaatga aaaactatgc tcaaaacgac atgagtgcga aagaaattgc
      481 agaaaatgcg ttaaatattg ccgcagatat ctgtatcttt actaatcata atattatcgt
      541 agaagaactg taagaggtga gtagatatgt atgaattaaa acaaacacca agacaaatcg
      601 taagtgaatt agatgaatat atcgtaggac agcacgcagc aaaaaaatct gttgccgttg
      661 ctttgcgtaa ccgctatcgc cgtttgcagt tagatgaaaa aatgcaacaa gatattacac
      721 caaaaaatat gctgatgatc ggacctacag gggtcggtaa aacagaaatc gcacgacgtt
      781 tggcaaaaat cgtcaatgca ccatttgtca aagtcgaagc aacaaaattt actgaagtcg
      841 gttatgtcgg aagagatgta gaatcaatgg ttcgggattt agtagaaaat gcgatccaaa
      901 tcgtagaaaa agagcagtac agccgtgtat acacaagtgc taaaaaaaat gcagaaaaac
      961 gtttggtaaa aatcttggta cctggtatca aaaaagaaca gaaacaatcc actaatcaat
     1021 ttgaacaaat gatgaatctt tttaatgcta gtcagcaaaa taccgctgaa ccgcaagaag
     1081 aagtgacaga agatattcga atcaatcgcc gtacagtctt ggaacaacta caaaagggtt
     1141 tactggatag tcgtgaagta accattgaag ttgaagacca aaagaaacct gcaccaatga
     1201 tgaacaatgg attggagcaa atggggatcg acctaaatga aactctaggt gctttgacac
     1261 cgaagaaaaa agtggaacgg acggtaacag tcaaagaagc tttagagtta ttgatcaagg
     1321 aagaatcagc aaaattagtc aaggatgctg atatccatag tgaggcaatc aaattagcgg
     1381 aagaaagtgg aattattttt atcgatgaaa tcgataagat cacttctaaa tcacagcaat
     1441 ctggagaagt atccagagaa ggcgtccaaa gagacatatt gccaattgtc gaaggttcac
     1501 aagtaaacac aaaatacggg atcatccaaa cagatcacgt cctattcatt gcttcaggag
     1561 ctttccattt gtctaaacca agtgatctaa ttccagaact tcaaggacgc ttcccgatac
     1621 gggtagaatt agatgatttg acagctgaag attttgtgaa gattctgacc gaaccaaata
     1681 atgctttagt aaaacaatac gtcgcgttga tcggtacaga aaatgtgtca gttacgttta
     1741 caaaagaagc aattgaacgg atcgctcaaa tcgctttcga tgtcaaccgt gacacagata
     1801 atatcggcgc acgcagactg cataccattt tggaaaaact actagaagat ctattatttg
     1861 aagcaccaga tatgcaaatg ggagaaataa cgatcacaga agcatatgtg gatgacaaac
     1921 ttggctcaat tgcggcaaat gaagatttaa gtcgctatat tttataaaaa atcggaggac
     1981 gacaatgaca gacttgctaa cgaaaacccg caagataaac acacttttgc agcaaaagaa
     2041 tacttttgac atgcaagcag atttgcctta cgataaaatg gctgtcacac taggtggtat
     2101 tcttgacagc aacacctata tcataggaag tgacggagta ttactaggtt ataatgaacg
     2161 tcacgatgtg aacaatgaac gaatcaaaac aatgtttgtt gagaaacaat ttcctgaatc
     2221 ttatactgat acagtggcac agttgatgaa aactgaagct aatatcccaa ttactagtga
     2281 tattactgca tttccggtag agtcaagaga gaaatatcca tttggtctga cgactgtcgt
     2341 tccgatcttt ggagctggcg aacgtttggg tacgattatc ttatctcgta tggaacaatc
     2401 gtttgcagat gacgatctag tcttagcaga atatggagct acggttgtcg gtatgcagat
     2461 cctttatcag aagtctcgaa gcatcgaagc agatgtacgg agtgcaacgg ctgtccaaat
     2521 ggcaatcaac acattatctt atagcgaatt gaaggctgta caagctatct ttgaagcatt
     2581 ggatggagat gaaggtcgtc tgactgcttc taatattgcc gatagtatcg gcatcactcg
     2641 ttctgtgatc gtcaatgctt tacgaaaatt ggaatcagct ggaatcattg aatctcgttc
     2701 gttaggcatg aagggaactt atttaaaagt attgaattcc cgttttaaag acgagttagc
     2761 aaaacacagt tattagaagt aaggggactg gggcagatag aaatgtttgt tccagccttc
     2821 ccattttaaa aagtaagtcg aagttggagg aatggcaatg actgttatta ttcaaaatga
     2881 ccagctgatc gccgaaatcg ctgaacacgg tgcggaattg atcagtctga aatcaaaaga
     2941 acatggttta gagtatatct ggcaaggtga tccagcgtat tggggcagac atgcaccggt
     3001 tttattccct tttgttggac gtttaaaaga tgatcagtat gtttatcaag ggaaaacata
     3061 tcaaatgggg caacacggat ttgctcggga tatggatttt gaagtcattg aacatggaca
     3121 agaaatggct agtttcatct taaggagtac gccggagaca ttagaaaagt atccatttga
     3181 ttttgaatta gtgatcagct atgaattagg aggagacggc atcacggttc attatcaagt
     3241 ggcaaatacc ggtgaaaaag aaatgttctt ttctatcggt ggacatcctg ctttcaatgt
     3301 acctcttgaa gaaggattgt catttgaaga tttctatctg gcattttctc cattgaaatc
     3361 aagattacgc ctaccattga aaggcccatt catcgatatg gatcaaaaga cgttaggtca
     3421 gacaaatacg aatctggctt tgaatcgtga actttttgag caagatgcct tggtttttga
     3481 aacaaggggt ctaaatgcgt acgccattcg aagtgaaaaa tcttcccata gcgtaacttt
     3541 aagctataaa gatatgcctt atgtagggat ctggtctcct tatccaaaag aagcgccttt
     3601 cgtttgtatc gaaccgtggt gcggaatcgc tgacacggtg aacagtagtg gaaacttgat
     3661 ggaaaaagta gggatcaatc aactaggtgc ccatgaaatc ttcaagacaa aatattccat
     3721 cacagtgaag taagaggttg tgaccgagaa gttagcaggg aacaccgttt accaaggtgt
     3781 gtaaaaaagc tatcctgtaa gtaataaggg agtgtgacat cgatttgtca cactcccgtt
     3841 ttatctcttt ggaatgaaaa agtatcagga ttttttcttg ctgctattca aaccaaacgg
     3901 gattcgactt tcagtatggt ttctgatacg cttgatattg tctttatgcc gataaaagat
     3961 aaaggaagaa actaaaaagg caatgatcgt cagtaaccaa ttgggcttag ccaaaatcac
     4021 aggagctaca taaggaagga cgatcgtaga taaagtgatc aatactgcac tgatcatact
     4081 gctcaaactc accatacttg tacaaaataa actaacgata aagatgatac aagaataaac
     4141 aaaaaataca ggattaaatc ctaaaagcat tcctgcactt gtcgcaacag cttttcctcc
     4201 tttgaaccca gcaaaaatcg gaaatgtatg gccaagaatg gcgcataccc caaaccaaag
     4261 agggttaatc ccagaaaggt ggaagatcat cggcaaacaa gtagccaatg taccttttaa
     4321 aatatccata agcaggacaa caatcccggc tgttttgcct aatacacgaa atgtattggt
     4381 cgttccagta ttaccgctcc cgtgttgtcg gatatctttt ttgaaaaaca atttgccaat
     4441 ccagacaccg gaaggaatag aacctaaaag atatgctaaa ataagtaata agatcaattt
     4501 cattttttac cctctttcaa gttgaaaact atcctttatt ttagcatgaa t
//
LOCUS       DAZAOG010000178         4513 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000178 DAZAOG010000000
VERSION     DAZAOG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.180"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..675)
                     /locus_tag="ACHDLD_002550"
     CDS             complement(64..675)
                     /locus_tag="ACHDLD_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3364214.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(798..923)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1005..1721)
                     /locus_tag="ACHDLD_002551"
     CDS             complement(1005..1721)
                     /locus_tag="ACHDLD_002551"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN3364215.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1727..2296)
                     /gene="scpB"
                     /locus_tag="ACHDLD_002552"
     CDS             complement(1727..2296)
                     /gene="scpB"
                     /locus_tag="ACHDLD_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN3364216.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2280..3050)
                     /locus_tag="ACHDLD_002553"
     CDS             complement(2280..3050)
                     /locus_tag="ACHDLD_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN3364217.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3047..3418)
                     /locus_tag="ACHDLD_002554"
     CDS             complement(3047..3418)
                     /locus_tag="ACHDLD_002554"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3364218.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3437..4324)
                     /gene="xerD"
                     /locus_tag="ACHDLD_002555"
     CDS             complement(3437..4324)
                     /gene="xerD"
                     /locus_tag="ACHDLD_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN3364219.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1223 a          983 c          789 g         1518 t
ORIGIN      
        1 attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg atctttttga
       61 tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac ttaaccatgg
      121 aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt tgatcaagtt
      181 gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca tctgtgttgc
      241 gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg cgatactcat
      301 aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc ttcctttatg
      361 ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg ttgataagaa
      421 actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca gatgtaatac
      481 tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca tgctgatcaa
      541 taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc
      601 aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca ttttctgtac
      661 acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa ctttttgacg
      721 tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag ttatttgatc
      781 aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt atcggaattt
      841 caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt
      901 tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca ttatacatat
      961 ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt tgagtggttc
     1021 agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg taggccttgt
     1081 aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc aactgcttga
     1141 agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac gatactgtgg
     1201 tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt tcgtccttcg
     1261 atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc ttttaccttt
     1321 gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca
     1381 tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac aggatagata
     1441 cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc atctgcaaca
     1501 gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc ttgataaatt
     1561 gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa ttcccttaca
     1621 acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt tcgacgagaa
     1681 gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat tctcctttat
     1741 tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt tccatttctt
     1801 gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag tagccagttg
     1861 ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca atcagtttat
     1921 gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag
     1981 ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg gcttgagata
     2041 atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct tttttagtcg
     2101 ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt
     2161 aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga tttaacagtc
     2221 cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg gcttcaagtt
     2281 cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat tgattcatag
     2341 ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa agccataaaa
     2401 gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc
     2461 gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg
     2521 ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat tttctgcatg
     2581 gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc agcatccgct
     2641 aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc tgctttttct
     2701 gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt gaccaattct
     2761 tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg gacaggaagc
     2821 aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc tcctgcaaca
     2881 gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt gatcgtagcg
     2941 atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag cagatcgaga
     3001 ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac tctttcttct
     3061 ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca cggatggaga
     3121 catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc tcaccgcgat
     3181 aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc cagtaaatac
     3241 caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt
     3301 cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc agtttcttat
     3361 ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt gttaacatct
     3421 tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc gtcattcttt
     3481 tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct aacaattcct
     3541 gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg
     3601 tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc agatttttcc
     3661 agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc atttcagatg
     3721 cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat
     3781 aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt accagtccaa
     3841 gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc atcccagtag
     3901 catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg
     3961 cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc tgtgctttct
     4021 tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc cgtaaaaact
     4081 gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct tttttttctt
     4141 catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc catgaagcaa
     4201 ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag ctttgtcgtg
     4261 tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg atttgctctt
     4321 tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt ctgatagtct
     4381 ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact ggtttctttt
     4441 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     4501 tttaggtatt aaa
//
LOCUS       DAZAOG010000179         4479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000179 DAZAOG010000000
VERSION     DAZAOG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.181"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(645..1148)
                     /locus_tag="ACHDLD_002556"
     CDS             complement(645..1148)
                     /locus_tag="ACHDLD_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3364220.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1507..2532
                     /locus_tag="ACHDLD_002557"
     CDS             1507..2532
                     /locus_tag="ACHDLD_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3364221.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2855..3733
                     /locus_tag="ACHDLD_002558"
     CDS             2855..3733
                     /locus_tag="ACHDLD_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFN3364222.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4209..4325
                     /locus_tag="ACHDLD_002559"
     CDS             4209..4325
                     /locus_tag="ACHDLD_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN3364223.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1497 a          699 c          830 g         1453 t
ORIGIN      
        1 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
       61 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
      121 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
      181 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      241 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      301 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      361 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      421 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      481 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      541 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      601 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      661 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      721 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      781 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      841 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      901 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      961 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
     1021 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
     1081 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1141 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1201 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1261 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1321 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1381 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1441 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1501 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1561 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1621 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1681 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1741 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1801 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1861 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1921 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1981 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     2041 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     2101 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2161 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2221 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2281 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2341 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2401 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2461 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2521 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2581 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2641 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2701 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2761 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2821 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2881 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2941 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     3001 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     3061 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3121 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3181 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3241 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3301 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3361 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3421 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3481 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3541 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3601 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3661 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3721 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3781 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3841 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3901 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3961 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     4021 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     4081 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4141 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4201 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4261 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4321 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4381 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4441 atgataaatt ggtagaatct ttcttttcaa attgatacc
//
LOCUS       DAZAOG010000180         4473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000180 DAZAOG010000000
VERSION     DAZAOG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.182"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1052
                     /locus_tag="ACHDLD_002560"
     CDS             324..1052
                     /locus_tag="ACHDLD_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3364224.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="ACHDLD_002561"
     CDS             1197..3224
                     /locus_tag="ACHDLD_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN3364225.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="ACHDLD_002562"
     CDS             3332..4429
                     /locus_tag="ACHDLD_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN3364226.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DAZAOG010000181         4445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000181 DAZAOG010000000
VERSION     DAZAOG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.183"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1656)
                     /locus_tag="ACHDLD_002563"
     CDS             complement(13..1656)
                     /locus_tag="ACHDLD_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3364227.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1653..2012)
                     /locus_tag="ACHDLD_002564"
     CDS             complement(1653..2012)
                     /locus_tag="ACHDLD_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364228.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2173..2433)
                     /locus_tag="ACHDLD_002565"
     CDS             complement(2173..2433)
                     /locus_tag="ACHDLD_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364229.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2424..2735)
                     /locus_tag="ACHDLD_002566"
     CDS             complement(2424..2735)
                     /locus_tag="ACHDLD_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3364230.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2736..3308)
                     /locus_tag="ACHDLD_002567"
     CDS             complement(2736..3308)
                     /locus_tag="ACHDLD_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364231.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3373..3600)
                     /locus_tag="ACHDLD_002568"
     CDS             complement(3373..3600)
                     /locus_tag="ACHDLD_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364232.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            complement(3641..3901)
                     /locus_tag="ACHDLD_002569"
     CDS             complement(3641..3901)
                     /locus_tag="ACHDLD_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFN3364233.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            complement(3891..4079)
                     /locus_tag="ACHDLD_002570"
     CDS             complement(3891..4079)
                     /locus_tag="ACHDLD_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364234.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
BASE COUNT         1302 a          950 c          634 g         1559 t
ORIGIN      
        1 aaggagtata ccttaaaatt caatctcatc caacatatca aatgcttcct cataatccat
       61 atcagttatt tcttctcgtt tatataaagc tgccaaaaaa gcatgaaacc catctgtttt
      121 acgccttact ggctcttttt tcaaaaatgt tttattccct aatttgtctt ctttgataaa
      181 cgaattattt gtataccaac gcatcatagg atcatcacca aaaataaacc gttcatttgc
      241 aaaaccatct tcaataatag gagcaacttt tgactgcaca cctcttatat ttctgataaa
      301 ctcaaaatta tatgattctt tctctagaag aggtttcagc aaatccatac gaaatccgtc
      361 ggcacaaacg atttctattt gatggatttt tcttttttca tttaaccaat caattaaatg
      421 acgaggatca attgatggct catcaacgat tgtcactaaa ccttttttct cccaatcctt
      481 aataggcgct ttcagtttga atgtatctag aaactttttc ctagcaaaac tatgttgctg
      541 ccagataaat ttatcatcaa ttttaaaaag caaacccact gttgcaaaat cacgaatact
      601 tgcataatca aatccagcca cacaagaacg accattcaaa tttggcaatt cttgtttagt
      661 agccattaat ttttcatgag tcgtaatatc tgattctgca tcaccttctg taaaattcat
      721 tcgtttaaca acaaattcac gcctacctga tggctcatct tctagttttt tatattcttt
      781 catcactgta gaaaacagac gttttgaata tgctgaatct tcttcgaaca ttggattagc
      841 ctttgaccac attttaggat tatccatttc atcaatgtta tccagtttac aaataaaggg
      901 aaacaagcga tcattcttgt tctctccact aaaaatcttt tgtgctcgtt ccaacatttt
      961 gtcataaaaa ccttctctaa catgaccatt tgtaccatta taaaaagttc gaggatgttt
     1021 tattttccct agcccactac gctgaatatc aactaggtct gaagattcaa actgatggat
     1081 ttcatcaaat tctaagcagc catctcttgc actatccata gtcttaggat tgtttgtcct
     1141 ataactaaaa atcgaattag ttacattgtt gactatagcc atcttagtca aataaaattg
     1201 tttttccagt ttgcgcattt gtaccatttc ataaacttct ttaaaagata cctttccctg
     1261 cttctcagaa ttcgctgtaa tcgttacatc ataatttctg acaccgtaga gagggctaat
     1321 aaaaaatgag tctcttgctg acatgaaccc atttttcccg ccgcctcgcg caatactatt
     1381 tagaatttca tcaaaaaaca cctcgtcgtc ctctttttga tagcagaaaa tgaaaggtgt
     1441 gataaatttt tgatatttag ctaaaggaaa aaaatttttt tcagtgtact tgataaactt
     1501 ttgaatcatt tcttcatcaa aatagatatc atcccgaact agcacttcgt tttttaaata
     1561 agtaattaac tgaattcttt ctttattaaa agggattacc cctttttcgt ataaatctac
     1621 gtattcatca aaatagaatg gctttaatgt tgtcacagca aatcactacc atctagcgct
     1681 gcattttttg taggttcgcg cttttcttct ggcaaatagt cagtaagttg cttaattaca
     1741 cgttgatatg cttggtctct cgaattatac tgtttagcaa ctggtcgttc ccgctcgtaa
     1801 ggtttttgat tttctgactg agagaaaagc tcataatcac ctttttcgga aatatcaatc
     1861 cacatttcat caagaagaat ttttaagcgt gccgcttgcg taatcaaacc agaaacgacc
     1921 ttctttttat ccttatcaat atccttaaac aatttattta gacgcctttc ttcagcagaa
     1981 acaagcctat ttctctcttg cagatcagcc atcaactcac ttcctttcta cgtggggagg
     2041 gggtcgtgtg aaaatagcat catttatttg cagagttgac cccatccacc ggttcccaaa
     2101 aattttattt cttctcaaaa tcttttgacc gggggtattt cactctatat atcttcgctt
     2161 tatactgatt tatcagccgt cttgctcgtt gctttctaca atatgtgcaa atggactcaa
     2221 ggtataatca atgtctacat taatattaaa tgtccctgtt acttcattag cgttacctat
     2281 tagtctattg ttgatagata tatcctctac agttgttgtt ccttcaatga gctgactctt
     2341 aaatagaacc aacttatcaa tagccttatc aaccatagcg attgcttctg ctcgtttgac
     2401 atcatctctg tttgctgttt ttcttaccac cattcatcat cccactttct tttcttttgt
     2461 tggccacgat atttcatacg gttgtgcctt ttgttatggc aatctttgca taaggttctt
     2521 aggttgttta tatctagagc taaatccgga tgatactcca gctcctttat atgatccact
     2581 tcaagaattg aatcatgttg agttgttagt ctaccctctg ctttacacca ttggcactca
     2641 taatgatctc gctctaagca ttgacttcta agtttcctcc actcagttga tccatagaac
     2701 ctagctcttg cttgtttact tgaaacatca atcatttaca tatctctctt gtgcccaatg
     2761 atttttctat ctaagtactt gttattatgt gaatcataat attcaatagt gatgtcgtta
     2821 gctccttttg gtacttggca ttcatattct tccgtatccc aatgataagt aatatcgaca
     2881 agacctatcg gcatctcatc cattcgttga cctttataat aaacttctgg tactgagtca
     2941 gtatctctta gtttgatttc tagaaggttc gtattacttc catcatctaa cgtcaattca
     3001 gcaatcctct ttttgatctg ttgaattact tttcttcctg tctcacctgt cattggtact
     3061 accgtctcat atccagtaaa ctcgcaagta aagcgttcaa tagcataagc gttttgaagt
     3121 ggcttagcac taagaacagc tggattattg ctataaaaac gatctactaa taacttccct
     3181 ataggaatgg catctaaagc tctatcagta aacactaaag cattgggata atcttcttga
     3241 attttgtaag ctagattggg tgcagttaca acgttatacc cttctctgat ataatctttt
     3301 aatttcatag ttttcctcct aaaaacatct aataattact tgctaacaac ttgatgtaat
     3361 catagttatc attcatttga tgtacctctc aatgttgtat tggatatact cgtctttcca
     3421 atagccatga ccgcaatata tcagcttgca tttatccact tcgtttggtg tagcttctct
     3481 tagcatttcg acaatagagt acttcccttt gatttgtaca gaacgcacaa cacgcactga
     3541 acaatcacca atggttcgag gatattcatt agttagtgat acataccagt agtttctcat
     3601 tatgcagcct cctttatgta aagaaaaagc ctagcacctg ctagactttg atacattcat
     3661 ttgatttttt gagataagta tctacataga tttcaccttt gtccccgttc aacgtaattt
     3721 caaaatacgg tgctcctttt aagctagcgc tgactaatgc tttgctattt tgcagtgttt
     3781 tgcatgacca gactatgaaa atactaaact caggcactgc tccacttagg tcagcaatct
     3841 cattttcaat tctttcacgt acaatagcct tgcatttttc gataaacttc tcatgattca
     3901 ttttgtacct ccgcaagcgc ataaccagct gttgatgtat tgttaaacgt agctgaacga
     3961 gtaactcccg tagccttccc agtataaata aaactaaatc ctgttgttgt tggcttaaaa
     4021 ttctccactt cataaaaatg atatgtttgt ccgttattag taaatacaat caattccatt
     4081 tttcaaaatc ctctcttttt taaaataaaa agaccactca aagagtgatc taatatgtaa
     4141 tgcacaggca gggacgtttc cgatcctgtg cttgagtcat ttgacgattc atttgtaccg
     4201 aatcccaaaa ctcaatctaa cccaaccaat tatgtaatag caacctacaa gatgcacaaa
     4261 acgcgtacga aattgcgcac ccctatattt ttaaaccgcc gatgcctcgg ttaccaaagt
     4321 cactggcaag gaatcgaacc ttgcagtttg cacctaaatt ttagatgagc gatacacctt
     4381 tccaccacag tgacataatg acaatagaca gcaactgatg atagataata agaacaattt
     4441 agaag
//
LOCUS       DAZAOG010000182         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000182 DAZAOG010000000
VERSION     DAZAOG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.184"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHDLD_002571"
     CDS             complement(543..2048)
                     /locus_tag="ACHDLD_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3364235.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHDLD_002572"
     CDS             complement(2045..2731)
                     /locus_tag="ACHDLD_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3364236.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHDLD_002573"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHDLD_002573"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN3364237.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAZAOG010000183         4425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000183 DAZAOG010000000
VERSION     DAZAOG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.185"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHDLD_002574"
     CDS             690..1658
                     /locus_tag="ACHDLD_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3364238.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACHDLD_002575"
     CDS             1669..2484
                     /locus_tag="ACHDLD_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFN3364239.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHDLD_002576"
     CDS             2552..3262
                     /locus_tag="ACHDLD_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3364240.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHDLD_002577"
     CDS             3298..>4425
                     /locus_tag="ACHDLD_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFN3364241.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZAOG010000184         4326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000184 DAZAOG010000000
VERSION     DAZAOG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.186"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACHDLD_002578"
     CDS             complement(<1..283)
                     /locus_tag="ACHDLD_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN3364242.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACHDLD_002579"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACHDLD_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACHDLD_002580"
     CDS             complement(2361..2723)
                     /locus_tag="ACHDLD_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364243.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACHDLD_002581"
     CDS             complement(2727..3035)
                     /locus_tag="ACHDLD_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364244.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACHDLD_002582"
     CDS             complement(3044..3463)
                     /locus_tag="ACHDLD_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364245.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACHDLD_002583"
     CDS             complement(3456..3689)
                     /locus_tag="ACHDLD_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364246.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="ACHDLD_002584"
     CDS             complement(3686..>4326)
                     /locus_tag="ACHDLD_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3364247.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DAZAOG010000185         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000185 DAZAOG010000000
VERSION     DAZAOG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.187"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHDLD_002585"
     CDS             complement(37..1257)
                     /locus_tag="ACHDLD_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3364248.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHDLD_002586"
     CDS             complement(1277..1888)
                     /locus_tag="ACHDLD_002586"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN3364249.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHDLD_002587"
     CDS             complement(1931..2863)
                     /locus_tag="ACHDLD_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN3364250.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHDLD_002588"
     CDS             complement(3284..4144)
                     /locus_tag="ACHDLD_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3364251.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZAOG010000186         4100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000186 DAZAOG010000000
VERSION     DAZAOG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.188"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1014)
                     /locus_tag="ACHDLD_002589"
     CDS             complement(<1..1014)
                     /locus_tag="ACHDLD_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFN3364252.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIED"
     gene            complement(1018..1428)
                     /locus_tag="ACHDLD_002590"
     CDS             complement(1018..1428)
                     /locus_tag="ACHDLD_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3364253.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1446..1937)
                     /locus_tag="ACHDLD_002591"
     CDS             complement(1446..1937)
                     /locus_tag="ACHDLD_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN3364254.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1947..2738)
                     /locus_tag="ACHDLD_002592"
     CDS             complement(1947..2738)
                     /locus_tag="ACHDLD_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3364255.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2731..3504)
                     /locus_tag="ACHDLD_002593"
     CDS             complement(2731..3504)
                     /locus_tag="ACHDLD_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3364256.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3660..>4100)
                     /locus_tag="ACHDLD_002594"
     CDS             complement(3660..>4100)
                     /locus_tag="ACHDLD_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3364257.1"
                     /translation="LENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLR
                     FLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKE
                     GAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1281 a          844 c          549 g         1426 t
ORIGIN      
        1 atcttcgatt ttaggaacaa ttactctttc tttttttcca aattctttct ctaacttaaa
       61 acttgaaaat ggagtgattt ctttaaaatt ctcaattatt ttttcaggca gtttaacacc
      121 aagagttttg tctgtctttt gaataatttg atgtggaaca agtgcaccgc cccaatccca
      181 gttattttga tcgttctccc cttcttttgt tgccacccag ccagttagaa aacggccagt
      241 ttcattagtt ccgactgttc ttgctgcata ataggctttt ccgtcgaaag cttcgtctac
      301 tggtgatttc caatctgaaa ataatcctgc tccgactcga tatttagtct ttttatcttc
      361 acaatactct gaaaaaagta aataccacgt tccatcaatt tcaaatatat caggcatttc
      421 aatcatgtta aattcattat tagcccagta gtctcctttg aaattccaat cttttaaatc
      481 ttcggattca aaatagacca atctccccgt cggctttttc ttacttgctg gttttctagc
      541 tcctagtatc aaaacgtatt ttccatgatc ttgatcaaaa attgcaaaag gatcgcgcca
      601 atcattctta tcatatcctt cttgaggaac aagttttgct gcttcgccat cctttttcca
      661 atgaattaaa tctttagaac ttgcaatcat taacacttct gaagtttttc catccaactt
      721 agctttgcga ttatgtccag tatacaaagc taaatactca ttgattcctt ggacaatact
      781 tccagcgtaa atgtattgat caatgtcatc gttgccacca cgatgaatta cttccccgta
      841 ttcttcaaat ttcacaaaat cagtggttgt aactagtgac cacccaaaag gatcagttaa
      901 aggtttagga tttctcgtat ctcgttgatg aaatagataa aaagttccat ctttataaaa
      961 aggcatgcaa tccccaaacc aaaaatcttt aggcttgtaa aaaatttttt gcatttatca
     1021 ctcatcctct tcgttagtat gtctttgcat atctgcaaga cacgcaattg tattcatatt
     1081 tcttagattt tcgatgatat catcgctttt ttttccttgt aatctcaaat tgacagcctc
     1141 aatcaaaatt cctaaattca tgccaccaag aagttgaaac ccattttgaa atacttcact
     1201 tcttttagca aattcgttga aaggtgttcc accaatcaag tcagcaaata ccataacttc
     1261 ttgatttttt acttgagaaa ctattttttc aatgttttca cggaattctt caatgcctgc
     1321 atctccaagt gttaccacat gacaatttaa attatcgcca aaaaacattt ttaatgtatc
     1381 tacaaatcca atggcaatat ttccatgaga tgtaattata aactccatcc actcatcctc
     1441 tgttcttaat aaataaaatc ctttaaaaat tcttttgaat ctctaggagt ttgtatcact
     1501 gtcacttcaa ctccatcact aattagttct ttaaaagcct ctctctcttc ttcagtaata
     1561 tttatcgtcg gacggatgcg ttctgttcca ttccttcttg ataaatttcc tacgtttaca
     1621 cttgttattg gtaaaccgtt atcaatcaaa aatttaacat cgattggaga aactgcaaca
     1681 ataagaactt tttgtccttc atatttacca gaaagaatat ttttaactgc tacttccctt
     1741 gtaattattg aagttctaac attaggtggt gcagcgaggc gaactacagc tttttttacg
     1801 tcatcattgg ctacttcatt attaataacc ataattctgt tagcactcaa ttgatttgtc
     1861 cattgtgttg ctacttgtcc atgaatcatt cgatcatcaa tacgaacatg tataatacct
     1921 ttcatttttt catccatatt caacacctac ttcaaaatat ttaacgctct taacgcaata
     1981 gaaactacta agattaatag aattacataa actggtttaa cctttcgtcc taacaaataa
     2041 tacacaattg ctactacaca tgcaggtaat aaaccaggca taacttgatc aaagatttca
     2101 tttccggtca ttgttaagtc gccttgttta aattcaaagc caacattcac ttttacaact
     2161 gtggcaatga gtgcaccgac caccattaac cctaaaattg atactgcatc agtaatagac
     2221 tttaattttg tttcaatttg agttagtagg ctcgcacccg attctttccc agcaaggtat
     2281 aaaggataag cagtaatcct taataaaatt gatgccagta tccacaaaat aattccaaca
     2341 aaactacctt ccaatgccat attagctgca attgctccaa aaatagtcca tggaataact
     2401 acaaatacac tatctccaat tcccgctaaa ggacccatta gacctgtttt cagagcactt
     2461 actgcttcca aagacttatt tttttcttct ttttcaattc caacattcat accaataatg
     2521 taaggcgctg tatacggcgt agtgttaaaa aattgtaagt gagctaaaac ggattgtttc
     2581 attccttcag gatcatcttt atatgtgtcc ttgataaaag gaagcataga atacgcataa
     2641 cccaaccctt ccattttttc ataatttaat gaagttgcac cattgagaat ccaccggaaa
     2701 attacttgtt tgtcgatttt tgtcagtttc ttattcatct tcgtcaaaac ctcctgttgc
     2761 agctggtgct acttgagtat tatttgatct atttttatat acgattagtg cgattgcagc
     2821 gccaagtaac gaaatcccta aaattggtat ttggaaataa actgccaaaa cgaaaccaac
     2881 taacaaatag gcaaagtaat cttttgtagg taaatattta agtagcattc ctatcccaac
     2941 agcaggtaat agtccccctg ctacctttaa accaccggta acccattcag gaatataatt
     3001 gattgcactt cccactaacg ttgggcctaa tatcacagtt aacaaaacag gaattccaga
     3061 tacaaagctt gtattaattg atgccaaata ttgcatccac tctaattttt gataatcttc
     3121 tttttgtgcg aatttttctg ccttttgttg aaaaaaaatg tttgttgacc actttagaac
     3181 atctagttgt acaattaaca ttgctaccgg tattgctaaa gttattccta tagaagcatc
     3241 ctggttagtt gagattgcta aataagtacc gatcaatgct gctgtttgat aatccggtac
     3301 tgatgcacca ccaaaactag taatacctag tgtcattaat tgcaaagtcc caccgatata
     3361 aagtccagtt tgaatatctc ccataataag tccggtcaag aaacctgcaa cgacaggttg
     3421 ggaggttccg attttgggcc cttccttatc ccagttaatg aaaaatccat acaacacaac
     3481 taaaaatgtt tgccaaaacg ccattttgtt ctctcctttc ttcttatact tttatataag
     3541 caattactgt gccaactaac attatccaat aaagcgggaa aatacagtaa atattaatta
     3601 aatgagcgtc taaatatact ttggacaccc tttcattttt ttagacgcta gtatgctatt
     3661 tagaaaatag tctgctcaaa aattgtatag attaactcat actctccttt gggtatttca
     3721 atacagtaaa tatctttgat ttctgaaaaa aaatcttttg atacatttaa aaaagtatca
     3781 aatagttgcg ctccctcttt caaatgttca tttgttattc catcaccaat cataattcgt
     3841 tcaatcattc cagaaagatg tagaaataga ttaattctca gaaaacttgg gattttgaca
     3901 tcgtaatatt tttcatacaa atatagtact ttttctatct cattgaatac ttttttggaa
     3961 tctaaaaagc gaagcttagt actagcgcct tcaatagtaa agagtttaac taattcattt
     4021 aagcagaact cacagctatc cgagtccata tacttattta atttttctaa ggtttgattc
     4081 ccattaatta gattttctaa
//
LOCUS       DAZAOG010000187         4037 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000187 DAZAOG010000000
VERSION     DAZAOG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.189"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..772
                     /locus_tag="ACHDLD_002595"
     CDS             176..772
                     /locus_tag="ACHDLD_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3364258.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            896..2695
                     /locus_tag="ACHDLD_002596"
     CDS             896..2695
                     /locus_tag="ACHDLD_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN3364259.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2973..3185)
                     /locus_tag="ACHDLD_002597"
     CDS             complement(2973..3185)
                     /locus_tag="ACHDLD_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364260.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3647..3796
                     /locus_tag="ACHDLD_002598"
     CDS             3647..3796
                     /locus_tag="ACHDLD_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364261.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1188 a          689 c          711 g         1449 t
ORIGIN      
        1 aaaaaaaaca agttattaaa taagtcttta gttggatgtt tttttgagat caatctttta
       61 tacttatgat gaacttacaa aattgtttgt tttctgtcat atcaaaaaat ggcgcagtta
      121 gttgcttttc tagtaaagta tagttaacaa agaacctcta aggagttgat gaataatgaa
      181 tattgctgcc tctcgaaaac tgataaattt catatccatt atcggattag gcctatcaat
      241 cgcgctgacg atttatttta ttaatttagg tgttttcaaa gatttgaatg cgttaagagg
      301 attagtaggt gattccatca ttttaggacc gattattttt gtcttcatcc aaatcctgca
      361 agtagttatt ccaatcattc ctggagggat cagtactgca gctggtgtcc tgatatttgg
      421 cccttatgct ggatttatct ataactatat tggaatctgc attggttcga ttatcatttt
      481 tctgctaggg cgtcgttatg gaaagccatt catactttcc atgattagcg ataagacata
      541 taacaaatat attggctggc tggataatca aaaccgtttt gagaagctgt ttgctctagc
      601 tatttttctt ccagttgctc ctgacgatgc gttatgtttg atggctggat tgacaaacat
      661 gtcagtgaaa cgatatacac taatcatttt gattgctaaa ccactgtcga ttttcttata
      721 tagtatggcc ttgatttatg gaggtcaata tctaagtggt cttcttggat aatatttatt
      781 ttgtaaaatt agtcattgat tgacaatgtt aaatctggga aagggtctgc aaaacacact
      841 gcaccctttc ctttttcatt agatttgcgc tacaatgtga agagcaggtg ataggatgaa
      901 ttatttgaaa cgtagcttag gtaatgcttg ggacttttta aaaggaacac aagcttattt
      961 ccgtgatgtc ttgctgatgc atggctttat tttatttatt tgtttaccct tattgagcag
     1021 tacgacccgt tttattttac accgaggggc aattgactat ttgtcttatg acaatattcc
     1081 ttcaattttt tctcaacatc ctggggttct actctcgtta atcgtagttt tgatgacgat
     1141 tttactgtta gtttatttcg aatttacttt tcttttgatg agtgtttttt ttattaaaaa
     1201 gaaagaacca atctctttga agcaactctt acacttgacg atacttcagc tgaagaaagt
     1261 gagacccatt acttttttgt ttttccttgc ttatttttta ttgattctac cgatcagcgg
     1321 attaagtttt aattctgatc tactgtcaaa aataaaaatc cccgctttca ttatggattt
     1381 tatttttgct aatcgttgga tcatcgtcag ttccttttta ctcgtttata ttttccttgg
     1441 ctatattggg attcgactga tttttgcttt accggaaatg attctacgag atcgtccttt
     1501 tcgagcagcc attcgggaaa gctggtcatt gaccaaatcg cgtcttttgg ccatcacggg
     1561 ccaatttatt gtgattggcg gtacgatttt actcctttct tctttaggat atatcgttgt
     1621 tattttggca caaagtatgg ttgaacaatt tttcccagac tatgcattga tcagcgctgt
     1681 gtttgctatg acgcttttac aagggatttt attattcaat atcgtgatgt ctaccgtagg
     1741 gattttttat atcattgttg atttcatgga tgacgaaggc ttcttgcctg agatccctaa
     1801 gtggtttatc ccacaagccc ccaacttgcg tttctctgcg ctgaaaaaca ctggtttgac
     1861 tctttttgca gtttttttcg gtattggcgt ctgtctttac aatatggatt atctgacttc
     1921 agctgttcag acaaaaccag tgactgtctc tcacagaggt gtgagtaccc aaaatggggc
     1981 gcaaaatact ttagaagcac ttgaaaaaac aagcagagac tatcatcctg attatgtaga
     2041 gatggatgtt caggaaacaa aagatggtca tttcgtagtg atgcatgatg caaatcttcg
     2101 tcacttaaca ggggtaaatg gaacaccaca ggatcttaca ttgaaagaat tgacaaatct
     2161 tacagttact gaaaacggag tgcaagcccc tgtttgttct tttgattctt atttggaaaa
     2221 agcacataag ctaaaccaaa aattacttat tgagatcaag acctctaaaa aagattcgga
     2281 tgaccttgtc gaacgatttg tccataaata ccgcaaagtt atcttagaga accatcatat
     2341 tttgcaaagt ttgacgtacc aaaccgtttc agatttaaaa gaagcagaac caaactttta
     2401 tgtgggctac atcttacctt tcaatattgt tgggccccct gtgacgcctg ctgattttct
     2461 gacaatggaa tatagtacga tcaaccgtaa cttcattaat tctgcccacc aagacggaaa
     2521 agaagtatat gtatggacaa tcaacgattc agatgctatg agtcgcatga tgttttatgg
     2581 tgtggatggt atcataaccg atgagatggg actactaaat gaaagtgtga aacaaacaga
     2641 tgaagaaatc acttattcag ataaactgct aaattttgtt ttgggagtcg gctaatcaaa
     2701 aatcgttaat gaaaaaagtc tttatacaac aaaaaacgca taacctgcca ccgcaggtat
     2761 atgcgttttt tgtttgacgc tcataatgtg tgcttaactt tctcttgatt ccttcatcgc
     2821 acgaacaata gctaaaatag caggaattgc agagatcact gcaagaataa ttcccatttt
     2881 ccctgaactt tttctttttg gcatatcttc tcgctccctt ctgtctttat ttatattata
     2941 agggagttct tgcgaattct caaaaatact gtttaagaaa ccaattcagc aggtgcgcta
     3001 atatcacaga attttacgag catgcgatac ttgctttcat tgataagttg atagtctcgt
     3061 tggtcataac tttcaacata cacgacagcc gtgttgatat atgtacgttc aatacagccg
     3121 attagttcat gagatactcc gatcggtttg catatataag atcttcctac ttctgctttt
     3181 accatttcat tccctctttt ctttattttt attttgtttt ttctttattt tataataaca
     3241 aattaccttg tatttgaaaa cgtttttatc cattttttta ttttttattt tgagtatctt
     3301 tctgcttttt agataactat tttttaacac gatcttagac gttgtgacaa aaacgtttgt
     3361 ttatattcct atgttgttcg tgcgtttttt tctttatatt gctaagttat ttataaagag
     3421 gtgagcatat gatttatatt caagcaatag tatttgtaac cttgatgatt gggctactta
     3481 tcggattaaa agaaaatagc taaaccattt aagaaaaaat aaataaggaa aatgataaat
     3541 gctttttaat tgcatcaaaa aacgtacgat gctaaacttg gtataagttc gaaagtttaa
     3601 aggaaacttt caagaactaa attacattac aaaggagatg aattttatga cgtttacaaa
     3661 aacaaatatg caataccgcg tagcttttga tacaacttct aaccgtttta tggcgattga
     3721 tgcacaaaac gaaaataacg ttgcgtttgg tattacaatc gaacaagcag tccgagcatt
     3781 aaacgaatct agataaggag tgaaccattc caaatggcga ctgatgagat ctcggaaaat
     3841 caataaaaga cgtttgtaac ggtaatccgc gacaaacgtc tttttgtatt cattacatat
     3901 atttggaaat tgtctctctt tatctctaga cctaaacaaa aacacaagtt ctaatatcaa
     3961 ccatctcgtt ttttatgaca attttgttag ctgtttcgat gataatattc cgttattatc
     4021 tgtataatat ttaatat
//
LOCUS       DAZAOG010000188         4010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000188 DAZAOG010000000
VERSION     DAZAOG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.190"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..574)
                     /locus_tag="ACHDLD_002599"
     CDS             complement(32..574)
                     /locus_tag="ACHDLD_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN3364262.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            709..1098
                     /locus_tag="ACHDLD_002600"
     CDS             709..1098
                     /locus_tag="ACHDLD_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364263.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1102..1866
                     /locus_tag="ACHDLD_002601"
     CDS             1102..1866
                     /locus_tag="ACHDLD_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3364264.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1995..2942
                     /locus_tag="ACHDLD_002602"
     CDS             1995..2942
                     /locus_tag="ACHDLD_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3364265.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3072..3812
                     /locus_tag="ACHDLD_002603"
     CDS             3072..3812
                     /locus_tag="ACHDLD_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364266.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1329 a          684 c          814 g         1183 t
ORIGIN      
        1 ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt ctcacttttt
       61 tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa gcacgctctt
      121 cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa agtatttcga
      181 gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca taaccgtgtt
      241 ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa atcgttgtga
      301 ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg atacctactg
      361 cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct aaaacaaggg
      421 ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca atcagtcgat
      481 agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca aaattaatga
      541 ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc atttatataa
      601 caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca tacaaaaaaa
      661 tgataaaatg attataatag aattataaag ataggttagg tgattaaaat gaaaacggtc
      721 aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata cttaggtgtc
      781 tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc gctgacagat
      841 ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat tttcggtagc
      901 gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt aggagtggtg
      961 ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat attatttggt
     1021 acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc aggaaattta
     1081 atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt
     1141 ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat
     1201 tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag
     1261 cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt
     1321 tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac
     1381 acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg
     1441 gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg
     1501 ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc
     1561 gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag
     1621 agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat
     1681 ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg
     1741 cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta
     1801 caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat
     1861 aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg
     1921 ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta
     1981 gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa
     2041 tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag
     2101 ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca
     2161 cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct
     2221 attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc
     2281 aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg
     2341 tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg
     2401 aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa
     2461 cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg
     2521 agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc
     2581 ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa
     2641 actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag
     2701 gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg
     2761 gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag
     2821 atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag
     2881 gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt
     2941 aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa aataatcagc
     3001 gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa
     3061 ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg
     3121 agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc
     3181 ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca
     3241 cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc
     3301 gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat
     3361 cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac
     3421 aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg
     3481 tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta
     3541 gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt
     3601 atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg
     3661 tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat
     3721 cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa
     3781 actctttggg agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca
     3841 gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg
     3901 tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg
     3961 gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt
//
LOCUS       DAZAOG010000189         3924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000189 DAZAOG010000000
VERSION     DAZAOG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.191"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACHDLD_002604"
     CDS             complement(573..1130)
                     /locus_tag="ACHDLD_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364267.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACHDLD_002605"
     CDS             complement(1309..1920)
                     /locus_tag="ACHDLD_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364268.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACHDLD_002606"
     CDS             complement(2033..2290)
                     /locus_tag="ACHDLD_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364269.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACHDLD_002607"
     CDS             complement(2887..3165)
                     /locus_tag="ACHDLD_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364270.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACHDLD_002608"
     CDS             complement(3199..3450)
                     /locus_tag="ACHDLD_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364271.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgta
//
LOCUS       DAZAOG010000190         3902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000190 DAZAOG010000000
VERSION     DAZAOG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.192"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..1431
                     /locus_tag="ACHDLD_002609"
     CDS             58..1431
                     /locus_tag="ACHDLD_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364272.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            1415..1852
                     /locus_tag="ACHDLD_002610"
     CDS             1415..1852
                     /locus_tag="ACHDLD_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN3364273.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(2132..3877)
                     /locus_tag="ACHDLD_002611"
     CDS             complement(2132..3877)
                     /locus_tag="ACHDLD_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN3364274.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
BASE COUNT         1078 a          723 c          807 g         1294 t
ORIGIN      
        1 tttaatgcta aaatcgaaga gcagattagt tataagactg tttaaggagc cattgtcatg
       61 ttcgaaaaac tcaaacgatt tgggcgtaat tttacacaaa caacgccctt tatcttaagt
      121 aacacggtta tttttattcc ttatctttta tttttgggac taacagactc gcataattgg
      181 ctgacgattt tgccatttac gctgttttat gcctttcgga tgactggact atttttagtc
      241 aacagtctac gatttggtct agatagttac acgttgctga tgatcgctct gctgattggt
      301 ggtgctggtt cattgcttgg aatattaggc gtattttatt ttcctttatt ttatttttca
      361 agtgttttgc tagggctgag tgcagcatgg cttgtacctg ctaataccac ggtcaatttt
      421 cacgaaaaga cgcaaggttt tatcaatatg gatgggaaaa agtacttatt tgcagctatc
      481 tggctatttc tcctgtatca agcgattgag ttgcctgcac cgactcagat tgcagcaagc
      541 tttactattt atacactttt ttacgtcatg gcctaccata cggttagtca ttatccgcgt
      601 tacgaactgg atttcaaaga catcaaaaga aatctggtag ctacgcgaga gcttgtttta
      661 tttttgatat tctttggctt attgtttctt ttgagaaatg cccgattgtt attcgatgaa
      721 aggctttttg atcttgctat ttatggtttc ttgctgctgt tcatcttgtt cgtgtttgtt
      781 ttaggacacc agaagaagga atggaagttg ccaagctggc taaatctgtt tacttttcta
      841 aatggaatgc tagggaattt ccttttttta tttagcagtt tttatgttgg catactgtac
      901 ggattagaaa aactttcgat ttttatgtat ttgccttatg cagcaggctt gattgcagca
      961 aaaatagtcg gaccatggat tttgaaacgt ttaacatggt ctccattggc tatgcaattg
     1021 cttggactga gttcttcttt attggtcctc ttgattccaa atggttttgc gataggtgtc
     1081 tttctgctta gtttctggca cgtagtaaca ggcagctggc taaataagga atattatcaa
     1141 actgatacag ctattccaat ggatcgccgg atcgttgtca agtatactac tcaaaataaa
     1201 gggagtgtga tccaccagtt tttattgatg acgcttttgt ttgttttagc aaaagaaatg
     1261 ggagagccaa tccatctatt attgatcacg ctgaaccatg aatcagtgcc gatagaaagt
     1321 gtacaactgc ttatcttggc aaaatggatc aatgttggtc ttttactgag cggattagta
     1381 actgtttatc atttatggaa gaaggcgaaa aagaatgagg ctattcattg atggagatgg
     1441 ttctccagta aaagaagatg tcataaaatt aggagaatcc tatggtcttg acgtggttat
     1501 cgtgaccagt gtggaccatt atacgaccaa agaatacgct aatcatgtcc aaatcgtcta
     1561 cgtagaccgc ggagctgata gtgcggatta taaaatcgtc ggtatggtta agaagaatga
     1621 ctttttgatc acgcaagatt atggtttagc ttcacttgta ttgccgaagg gtgtgcgagt
     1681 gttccatcac tctggaaaag aatttgacga agcgaatatc ggcacgcttc tagaacaacg
     1741 ttatcatagt ggaaagttaa gaaaagctgg cataaggaca aagggtccaa aaccctttac
     1801 ggagaaagat catatgtctt ttcgtcaagc gttaaaaaag gcaattgact aaggtttacc
     1861 aagtacagaa gatagtaggt aacgaaggta ttttatttat gctcgaatac atcaaaaagg
     1921 gaacagggaa acaggataaa tgtgttccat gttccctttt tgattgttct tattactggg
     1981 tgcagaacag ctttttcaca ttttttattc tcggtgtttc agaagtagcc tcttcgaaaa
     2041 ataagccgaa ttttcacaaa aatttgagaa acaatttatg taaaacccct tcttatttct
     2101 tgaagctgat tctttctgtc acaacctttt tctacttttt cctcttctta tacatttgga
     2161 tcaatgcatc gattgccaaa aaaataatga cgaatgcggc aatagcatac gtaaatatcc
     2221 ctaataagga aacgacctct gcgttttcta caggtgctgc ttggacaagc agggaggacc
     2281 ctacgatcaa tgcagctaaa atcataccga aaacaagccg attgatcata ctttcgatcc
     2341 tgctcaataa tttgtcttgc tttttcaatt ccaaattgac ttttccttgt cccgaagtcc
     2401 acatttctaa tagatggtgg agctgttgag ggatcttagg cgcagccttc atcccttcca
     2461 ataagtctaa tccgctttgt ttcaatgtat cttctacatc cagatgagtg agaaaataat
     2521 gttgagcaaa aggcgaagcg acttccatga gggaaacttc aggatcgagg acgcggatca
     2581 ccccttctaa tgtcccaaat gctttcagca aaagcgtgat atcacgatcg aactgcagat
     2641 tattttgatg acaaatcgtc acgacatgtg tgaatacttc ttgcaagttg atttcatcaa
     2701 taggagaatc ataatactgt tcaagaaaac ttgtcagttc ttggtggaac cgactttcat
     2761 caaacgagcc ttcttgctga caaagtcgaa ggaccgcttt ttcgattcga taagcatctt
     2821 tcgtatacag cgctaaaacg gcttgtgtga gtttttgcct aagtcctgca gaaagatgcc
     2881 ccatcatacc aaaatcgatg tagttgatgg tatatggcgc aacagggtct tctgcagaag
     2941 tggaggctcg aaaagcaaag gaaccgaact ctttttcatg gactgtttca gtttttcggc
     3001 tagcttgtgt ctgttcttct tttgttagta catggaatag cagattccct ggatgaggat
     3061 cagcatggaa aaaaccatca tcaaacacct gtttcataaa gttctcaact aaaagctttg
     3121 caacttcttg tttcagctgc ttgttttgaa ttccagtgat aaaagtttca gttttgtttt
     3181 cggcgttcat taaatgattc aagtttttac cagacatttc ttccatcacg atcacttttt
     3241 tcgaacaaaa agcgtcataa atcttggggc tgcggatctc tttccagcca ttgttctttt
     3301 gatagaattg ttcgccattt tgactttctt ttaagaagtc catttcattg atcaaggaac
     3361 gttttacttc ttggagtacc ccttttagat caactacgct agtttcagga atgtatttga
     3421 tcaaagggat cgctcgttca aatagttgaa gatcaagtcg gatctcttcg gcgatgttag
     3481 ggtgttggat cttaacgact accgcgtcac cgttcttcag cttggcacga tgagcctgcc
     3541 ccatagaagc agaggcaaaa ggcgattttg aaaaatcatc gaatatatct gataatgaaa
     3601 gagaaagttc tgtttcgatc gtctttttga ccgttgaaaa tgtgtcgcta ggtacactat
     3661 cttgtaatgt tttgaatgtt tgagtgaagg cagaagaaag caaatcttct cgaacagaaa
     3721 gcatctgtcc tatcttgatg aaggtggggc ctagttcttc aaaggctttt tttacctgtt
     3781 cgggattttt ttgctgtaca aagtttggca ctacattgta attgatgaac actcgaatga
     3841 tttctgccaa gcggctgcgt ggatttgttt gcgccatcat catccctcct tttctttatc
     3901 at
//
LOCUS       DAZAOG010000191         3876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000191 DAZAOG010000000
VERSION     DAZAOG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.193"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACHDLD_002612"
     CDS             158..2257
                     /locus_tag="ACHDLD_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN3364275.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACHDLD_002613"
     CDS             2453..3316
                     /locus_tag="ACHDLD_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN3364276.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACHDLD_002614"
     CDS             3421..>3876
                     /locus_tag="ACHDLD_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3364277.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZAOG010000192         3779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000192 DAZAOG010000000
VERSION     DAZAOG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.194"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1304
                     /locus_tag="ACHDLD_002615"
     CDS             <1..1304
                     /locus_tag="ACHDLD_002615"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN3364278.1"
                     /translation="NVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKK
                     LGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPH
                     MVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTY
                     NLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLD
                     DSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLE
                     DGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEEN
                     IQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIK
                     KIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1294..2511
                     /locus_tag="ACHDLD_002616"
     CDS             1294..2511
                     /locus_tag="ACHDLD_002616"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3364279.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2524..>3779
                     /locus_tag="ACHDLD_002617"
     CDS             2524..>3779
                     /locus_tag="ACHDLD_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN3364280.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1306 a          650 c          789 g         1034 t
ORIGIN      
        1 tcaacgtctt agcggatcaa gcaaaacaag caacgttcca attgaacgat ctaaacaaag
       61 cctttgtgca attggcatct acctacaatc aatttaatgg gttatttgat gatgtggatc
      121 tccaatcaaa aaaactagga acagatgagc aacaacgaaa tgtgacgatt accgaagtca
      181 tcaagaaatt gaatgatgta gatgttttag aacacgatgg ggacgtgatc ggggatgcgt
      241 atgagtttct gatcagtcaa tttgcttcag aagcagggaa gaaagccgga gaattttaca
      301 cacctcatat ggtttctgat atgatggcgc aaatcgtaac gctcgatcaa aaggaacgac
      361 gattctttag tgtttttgat ccaacgatgg gttcaggttc attgatgtta aacgtacgta
      421 actacttaac acacccagat aacgttaaat atcatgggca agaactaaat acaacgacat
      481 acaatctagc aaaaatgaac ttgattttac atggtgtcga cgccgaagaa atgaatctcc
      541 gcaatgggga tacgttgaat aaggactggc caacagatga accttacaca tttgatgcag
      601 tagtaatgaa cccaccttat tcggctaatt ggtcagcaga tacgactttc ttagatgatt
      661 ctcggtttaa tcgttatggg aaattagcgc caaaatccaa agcagatttt gcgtttcttc
      721 ttcatgggtt ctatcatttg aaggaaacag gtaccatggc catcgtctta ccacacggag
      781 ttttattccg tggcgctgca gaaggggtca tccgtcaaaa attactagaa gatggcagta
      841 tttatgctgt gatcggtatg cctgcgaact tgttctttgg gacatcgatt ccaacgacgg
      901 tgattgtgtt gaagaagaat cgtcaaactc gagatgtatt attcattgat gctagtcgtg
      961 aatttgttaa agggaaaaat caaaataaac tatcggaaga aaacattcaa aaaatcctcg
     1021 aaacctacgc tgaaagaaaa gatgtcgaga aatacgctca tttggcaacc tttgatgaaa
     1081 tcaaagaaaa tgactataac ttaaatatcc cacgctatgt agatactttc gaagaagaag
     1141 aaccaattga tatggttcat gtcggaaatg acatcaagaa gatacgacaa gaacagcaag
     1201 tgctagaaaa aaaactttta gaagctatct cttccctgca aacaacacct gaaaatgaag
     1261 cgtggttaca aggtgcgctg gaggtgttca aacatgagca atgatacaca accagaaatc
     1321 cgtttcccag gatttacaga tgattgggaa cagaggaagt tgggggactt cgttgtagat
     1381 tatgttgaaa agacaagtgt acaaaatcaa tttcctatgc taacttcatc tcaacaaaaa
     1441 gggatagttt tgcaagaaga ctattttgca aatcggcaag taactacaga aaataatatt
     1501 ggttactttg tactacctag aggatatttc acgttccgta gtcgaagtga taatgatgtg
     1561 tttgtgttta accgtaatga cattattgat agagggatta taagctattt ttatcctgta
     1621 tttactttaa aatctgctga ttccgacttc ttcttaagac gtattaacaa tggaatccaa
     1681 cgacaattat ctattcaagc agagggaaca ggtcaacatg ttctgtctct aaaaaagttt
     1741 aaaaatatag tagcaatgtt tcctagtgag gaagaacaac aaaaaatcgg caccttcttc
     1801 aaacaacttg acgacacgat cgctcttcat cagcgtaagt tagatttgct gaaggaaacg
     1861 aaaaaaggat tcttacaaaa aatgttccct gaaaacggag caaaagtgcc agaaatccgg
     1921 tttcctggtt ttacggaaga ttgggaagag cgtaagttgg gagaattagg taaaactcaa
     1981 tcgggaattg gatttcctga tgccgagcaa ggtggttctg aaggtactcc attttttaaa
     2041 gtttccgaca tgaataacat tggtaatgag tatgaaatga gaaatgcaaa tcattatgta
     2101 tctaatgagc aaattgagcg taaaaaatgg aagccaataa aagatgtgcc agcgattata
     2161 tttgctaaag ttggcgcagc aataatgctt aatagaaaac gtttggtcac aagccctttc
     2221 ttaatagata acaataccat ggcttattta tttaataata cttgggatat ctattttggt
     2281 aaaatattgt ttgaaactat aaatttgcca agatactccc aagtaggggc attgccttct
     2341 tataacagtt ctgatattga gaatatcagt gttaaggttc ctgtaaagga tgaacaacaa
     2401 aaaatcggta ctttcttcaa acagctagat gatacgatcg ctcttcatca acgtaagtta
     2461 gatttactaa aagaaacgaa aaaaagcttc ttacaaaaga tgtttgtcta ggagggaaga
     2521 agaatgaaaa ttagtcaccg tgaggaagca gaagtcgaag aacagttgat tcgtgtactt
     2581 ggagaagggc ataatcagtg gacgtatcgt ccggatctaa aatcagaaga agacctttgg
     2641 gtcaatttac gccaaaaaat tatctcaaat aatcaagcag aactgaatga ttttcctttg
     2701 accgataaag aatttgaaac gatcaaaacg gaattactgc tgcgaacgaa aaccccattt
     2761 gatgctgcga aatggttaaa aggtgaaaat gggatggctc ggatcacgat tgaacgggaa
     2821 gattcgcaat tgggttctgt ctcacttatc ctttattcca atcaagacat tggtggagga
     2881 atctctactt atgaagtggt ccatcaaatt gcaaaacgtg gaagtaacat agaagcacgg
     2941 gatcgacgtt ttgacgtcac cttattaatc aatggattac cgattgtcca aatcgagctg
     3001 aaacaagtaa ctgcaaaaga tggtgtttat caggcattta accaaatcaa aaaatatgca
     3061 gaagaaggta tgtttcggaa caatatcttt tcgaccttac agttgtttgt ggtttccaat
     3121 gaacaaacca ctcggtattt tgcaaatgcc ttaccgaaag acttgcaccc taagttttta
     3181 tttagttggc gaacgaaaga caatgaaaaa gtagaaaact tatatgagtt ttgtaaacaa
     3241 gtgttaaata tcccggatgc tcatcgtttg attgcggatt atacaatcgt cagtgaagat
     3301 caagacaata aaaccctcat ggtgttacat ccttatcaag tacatgcgat tcaagccttg
     3361 tttattgccg caaataaaca tcaatcaggg tatgtgtggc atgcgactgg ttcaggaaag
     3421 acgttgacta gttttgtatc tactaagctt ttagcacgaa aatcggggat cgatcgaacg
     3481 atcatgttag tggaccgcaa agatttagat aatcaaacca ccacagagtt tacaaaattt
     3541 gcttcggaat ttaatactgg gatttcttcc gggaacgcca aagcgaatag cttgatcgtt
     3601 ggaacgggaa gtgccaaaga attaagtgaa accctgttgg cagatgccaa tgcaaatgtt
     3661 gtaatcatta caacccgtca aaaactagat gcggcgttga aatatgcaaa gaaacaagaa
     3721 gagaaaaaag gaaccaatcg cttccaaaaa ttaatgggcc aacatatcgt gtttgtagt
//
LOCUS       DAZAOG010000193         3736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000193 DAZAOG010000000
VERSION     DAZAOG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.195"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..638
                     /gene="purE"
                     /locus_tag="ACHDLD_002618"
     CDS             150..638
                     /gene="purE"
                     /locus_tag="ACHDLD_002618"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN3364281.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            631..1755
                     /gene="purK"
                     /locus_tag="ACHDLD_002619"
     CDS             631..1755
                     /gene="purK"
                     /locus_tag="ACHDLD_002619"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN3364282.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1768..3063
                     /gene="purB"
                     /locus_tag="ACHDLD_002620"
     CDS             1768..3063
                     /gene="purB"
                     /locus_tag="ACHDLD_002620"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN3364283.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3243..3620)
                     /locus_tag="ACHDLD_002621"
     CDS             complement(3243..3620)
                     /locus_tag="ACHDLD_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364284.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT         1189 a          659 c          842 g         1046 t
ORIGIN      
        1 atgtgaccta acttttgtca cattcctttt tttcatcttt aataaacacg aacaataaaa
       61 tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata
      121 tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga
      181 gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt
      241 cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac
      301 aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc
      361 tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac
      421 gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg
      481 tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga
      541 tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg
      601 ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg
      661 atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg
      721 ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat
      781 tggcacattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca
      841 gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc
      901 atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa
      961 aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg
     1021 acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga
     1081 ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc
     1141 atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag
     1201 atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa
     1261 aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat
     1321 gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt
     1381 gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct
     1441 cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag tcagttcgat
     1501 gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat
     1561 gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat
     1621 aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg
     1681 ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg
     1741 aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg
     1801 aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat
     1861 gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat
     1921 gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt
     1981 gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt
     2041 ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat
     2101 attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac
     2161 aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga
     2221 ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg
     2281 gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg
     2341 ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatctctact
     2401 caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca
     2461 agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta
     2521 gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg
     2581 atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc
     2641 tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc
     2701 atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt
     2761 aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg
     2821 atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct
     2881 tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg
     2941 ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc
     3001 tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa
     3061 taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga
     3121 agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat caataatagg
     3181 aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg
     3241 aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc
     3301 aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa
     3361 tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct
     3421 gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt
     3481 tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc
     3541 aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct
     3601 tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt
     3661 taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt
     3721 cgtttctgat aaaaca
//
LOCUS       DAZAOG010000194         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000194 DAZAOG010000000
VERSION     DAZAOG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.196"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACHDLD_002622"
     CDS             102..2543
                     /locus_tag="ACHDLD_002622"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN3364285.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACHDLD_002623"
     CDS             complement(2873..3577)
                     /locus_tag="ACHDLD_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364286.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1058 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaa
//
LOCUS       DAZAOG010000195         3712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000195 DAZAOG010000000
VERSION     DAZAOG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.197"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHDLD_002624"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHDLD_002624"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN3364287.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHDLD_002625"
     CDS             complement(2260..2511)
                     /locus_tag="ACHDLD_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN3364288.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHDLD_002626"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHDLD_002626"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN3364289.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHDLD_002627"
     CDS             3461..3679
                     /locus_tag="ACHDLD_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364290.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DAZAOG010000196         3708 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000196 DAZAOG010000000
VERSION     DAZAOG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.198"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..349)
                     /locus_tag="ACHDLD_002628"
     CDS             complement(185..349)
                     /locus_tag="ACHDLD_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364291.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            644..1972
                     /locus_tag="ACHDLD_002629"
     CDS             644..1972
                     /locus_tag="ACHDLD_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN3364292.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2174..3007
                     /locus_tag="ACHDLD_002630"
     CDS             2174..3007
                     /locus_tag="ACHDLD_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN3364293.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3023..>3708
                     /locus_tag="ACHDLD_002631"
     CDS             3023..>3708
                     /locus_tag="ACHDLD_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN3364294.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIR"
BASE COUNT         1226 a          589 c          773 g         1120 t
ORIGIN      
        1 catgactgtt aatcatgatg tcgtaggttc gagtcctact gccggagtaa ataaagctta
       61 tgcaaagaaa aagaaatcca atcacttggg tttctttttc ttttattatt atgatgaatc
      121 aagctttctg tttttgtcac gtatgttgta atctttacga aagagtagag ttggctactc
      181 ataatcaacc atacttccca tcatcgctaa atacgaaata aatcagcatt cctacaccaa
      241 tcaaagcaca agctgcacca agaaaaggga tgacattccc aaagttatct gtattcataa
      301 gtatgccacc acccacaaac aaaaagccgc cagcaatcag gtttttcatt ttattcatct
      361 cctttcttac gttatagtat ttccttcttt caatgagtat tttgaaagct atcttattgt
      421 atgatgttct tctaggcctc ttctttatta tacaggccat tttcgttatc tcatagaaat
      481 attccttttt atctgaaaac tattgtatac cgtatgacat ttttcttcat gaagttgtaa
      541 gtatattctc attcaaatga aaatttcatc atagaagaga ttgtatttct aaattcttaa
      601 gagtaaaata cgaagtataa cttggatttg gaaggatgat tggatgaaag ctattacgcc
      661 tgatatgaca acaaaattta acaaacagtt tcaagcaaat atcaaacagc aggcattgca
      721 gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa aatgtttcag cacatgtgga
      781 gaatacaccg gtattttcta tagatttggc tacggggaaa gtggctaatc aaatgcaatc
      841 ggggcgttgc tggatgtttg ctgcactgaa cacattccgt cataagatgc tgaatcaatt
      901 ccatttaaaa gagtttgaat tatcacaaaa ttacactttt ttctgggata aatatgaaaa
      961 ggcaaattat ttctatgaaa acatcattgc aacaggaaaa gaggcaatcg atagccggaa
     1021 agtagctttc ttactcgcta ctcctcaaca agatggcggc caatgggata tgatcgtttc
     1081 attattccaa aaatatggcg ttgtgcctaa atcggtgatg ccggaaagtg cgaatagttc
     1141 aaattctcga gatttgaata actacttgaa caaattactg cgtaaagatg cagtagtgtt
     1201 acggaaaatg gttgctcaag gagaaacatt agacgctatt gaagaaaaga aagaagaaat
     1261 gctagaaagc atttataact tcttagctat ctcattagga acacctccaa aagaattcga
     1321 ttttgagtac cgcgatgaag aaaagaacta tcatcttgac cgaggactga caccacaaat
     1381 tttttatgat aaatacatcg gtgtaaaatt agatgattat gttagcgtga tcaatgcacc
     1441 aacaaaagac aaaccattca atcgttctta tacagtagag atgctaggaa atgtcgttgg
     1501 cgggaaagaa gtcaaatatc tgaatgttga tatggaaact ttcaaaaaat tagcgattgc
     1561 tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat gtaggtcaat cttctactag
     1621 aactagtggc atcatggcct tagatgctta ttcaatggat gatctatttg atacggattt
     1681 tacaatgact aaagctgaac gtttagattt tggagaaagt ctgatgaccc atgcgatggt
     1741 attgaccggt gttgacttag tagacgggca atcaacaaaa tggaaagtcg aaaatagctg
     1801 gggcgaaaaa gttggaaaga atggcttctt tgttatgagt gatgcctgga tggacgaata
     1861 tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa gagcaattaa atgcgttcaa
     1921 tgaagaacca attgttctat ctccgtggga tccaatgggt gctttagcat agacgagtat
     1981 ttcaatgaag agtatgtagt aaagataaaa agagctggct taagccagct ctttttatct
     2041 ttaaagtcca ttccctgttt tttatggtcc ctgtgctata attctaaata aaggataagg
     2101 attggaaata atccttttat cattaaaaat atgatgacga agtagaataa aattgggagt
     2161 aggggggaaa gtcatggata tccaaactgt tttatctgat ctgaaattaa atggtaaagt
     2221 gattccagtt gttggtggcg atgttaatca gacctatcgt ataaaaaccg aacatcgtgc
     2281 ttatttttta aaaatccatc cgaatgtaaa aaaaggattt tttgaagcag aagtagacgg
     2341 attaaaagaa ctgtcagcat tcgtacgtgt accggatacg tatatgctag gtgaaacaag
     2401 tgaaggcgct tatttgttaa tggaatggat cgagccggga aaaggggatc aacgagatct
     2461 tgctgcagct ttagctaatc tccatcagca aacagcgcca caatttggtt ttcgtaaaga
     2521 taactatttg ggtacacttg ttcagaaaaa ttcttttgaa gaagattggt ggactttttt
     2581 ctttaaagat cggcttgaat cccagatttc tttggcagaa gaaacgaatc gatggaatgt
     2641 acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta ttgaaatcgg ttgaaccaaa
     2701 aaagatcact ccacgcctct tgcacggaga cctgtggagt ggtaatgtgt tctttgacca
     2761 gcaagggcat ccagtttttg tcgacccagc tgtttcttat gggaatcggg aacaagatat
     2821 tgcaatgagc cagttattcg gtggtttccg accggaattt ttagatgctt atcaaactat
     2881 atttccatta gaaaagggat ggaaagaccg actgccgatt taccagttat attatctttt
     2941 ggcacatttg aatatgtttg gagaaagtta cggttcccaa gtagatcaat tactagaaaa
     3001 tttttaagag gagggcgtgt ttttggcaaa aaaatatacc aaaaagcatg aagtttcata
     3061 ttatgagtgt gacatcaacc aaacgatgac atttccttcg atgttaggag tagttatcaa
     3121 aacgtctgaa gatcaaagcg accagttggg aagaggcagt gaattcgtca atagctttgg
     3181 acttacttgg gtcatcacga attattccat ggagattaat cgtctcccta gagtcggtga
     3241 agtaatctca gtaacgactc aagccatgga gtacaataag tttttctgtt atcggaactt
     3301 ctggatccat gatgaagatg ggaaagaatt agtcaaaata gaatctgttt tcgtattgat
     3361 ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag atcattgctc cttttgagag
     3421 tgagaagatc aagaaaatca agcgtcagga aaaaattgaa aagattgatt cgggcttcat
     3481 gttgccatat cgtgttcgtt tttatgatat cgatagtaat cagcatgtta ataatgcgat
     3541 gtattttaat tggatcattg acgtattagg atatgacttt ttgactacgc atgttccaga
     3601 aaaagtaatt attcgatttg ataaagaagt agagtacggg aatgagatcg aaagccattt
     3661 tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat gaaatccg
//
LOCUS       DAZAOG010000197         3671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000197 DAZAOG010000000
VERSION     DAZAOG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.199"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHDLD_002632"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHDLD_002632"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN3364295.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHDLD_002633"
     CDS             complement(1172..1867)
                     /locus_tag="ACHDLD_002633"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN3364296.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHDLD_002634"
     CDS             complement(1880..2596)
                     /locus_tag="ACHDLD_002634"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN3364297.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHDLD_002635"
     CDS             2926..3534
                     /locus_tag="ACHDLD_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN3364298.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1049 a          755 c          659 g         1208 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc a
//
LOCUS       DAZAOG010000198         3631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000198 DAZAOG010000000
VERSION     DAZAOG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.200"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..1422
                     /locus_tag="ACHDLD_002636"
     CDS             382..1422
                     /locus_tag="ACHDLD_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN3364299.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2246..2578
                     /gene="traE"
                     /locus_tag="ACHDLD_002637"
     CDS             2246..2578
                     /gene="traE"
                     /locus_tag="ACHDLD_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN3364300.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3197..3538
                     /locus_tag="ACHDLD_002638"
     CDS             3197..3538
                     /locus_tag="ACHDLD_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN3364301.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1343 a          477 c          673 g         1138 t
ORIGIN      
        1 cttgtcttat aagaataata taatatatac tactgaaata aggtagtata tattactagt
       61 aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt tttgacactg
      121 gtagtactta attatcttta attttaaaac taaattttat tcatacgttt ttctggtagt
      181 atttaattat aaataatatt tatttaaagt agttcttaat gactttaaag gtagtacata
      241 attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta atgcgtttgc
      301 attttttatt tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa
      361 cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac
      421 aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg
      481 aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc
      541 aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt
      601 agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt
      661 ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat
      721 agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt
      781 gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc
      841 gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc
      901 caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta
      961 gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta
     1021 gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta
     1081 ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact
     1141 attcataatg caaagaaaaa agctgaaata gaaagtgata tttcgatagt ttttgaagaa
     1201 ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat
     1261 caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa
     1321 aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat
     1381 gcaagctccc caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg
     1441 attgttgctt catggagtaa cattaaaatt aaaaattttt tattattcta attatattac
     1501 gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat tatgataaaa
     1561 atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag
     1621 aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag
     1681 ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga
     1741 atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg
     1801 attttcgttt tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa
     1861 ggcggtatga actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt
     1921 tggcccttaa tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa
     1981 cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat
     2041 tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta
     2101 tgacgaacgc tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt
     2161 catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaacttg ttaaaaatat
     2221 ttaaagaaat tgggtggttt attttatgtc atatacagga attttgagtc tcaaagatat
     2281 ttgtcactac ggtaaacggt gtaccgctac agagaaaatc acgaagaaac tatcaactgg
     2341 ccaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca aagtctcaga
     2401 ggaaatgatt tattacattg ggaaacaaaa gcaaataatt ttaaaagatc ctatcccctt
     2461 aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg
     2521 aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga tcggctgaat
     2581 tgatcattct cataacttgt attttatcgt ttttcgcttg cttgaaaata ttgtcgagac
     2641 ttctaaagga acttagagtc actagaaaaa taaggagtag acttgatcta tcaacaattc
     2701 ccttcgggga gtgacggggt aggttttaac gattggtaac gtaaaaaatt ggtcaatcga
     2761 gtaaatgcgc ttgtttggat agtggtgaaa tgaatttgtg taaataataa gcgcagagta
     2821 aacaagaggg aatttagaag ttagacaagt tagaatagcg tctagattaa tcatgagcag
     2881 ctactaaggg atctagactt tgacgcaaac tcatggaata aggagtaggt cttctcgtaa
     2941 tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata cttgcgagga
     3001 cgttgattat tggatcaatc gagcatttac cgctgtctac tagggtttct cgtttcggac
     3061 gccataaacg agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg ataaaatatg
     3121 aacaagtcct gaataaagtg aaaaaattaa tataaagcat agagaaaatg aaatttacgt
     3181 aatggaggaa tgacaaatga aaaattatga tccgaatata cgattgggca cacatactat
     3241 taaggtttct ttccaacgtt gggattacaa aggatttgtg acgtttagaa ggggcggaaa
     3301 ttgtaaagga ttagatgttt tagcattaga cgaggatgat ttatacgatc aaacattgac
     3361 tgataatcca attggattcg gcttgctacc tgaagatgat gaaggtaatg aatggtttaa
     3421 aatgacgctt atgaatgata atggagacga attatctgtt gaagatactt ggtcatattt
     3481 gagtgattat attgtcagct ttgaaatcat tgaatttgta gcggataaag aagaataaaa
     3541 actggaggaa taaattttac agataaaggc ttagaaggag tcacgacgat taataaaaag
     3601 gtagtattac cttaggcatt gagtaaggaa t
//
LOCUS       DAZAOG010000199         3618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000199 DAZAOG010000000
VERSION     DAZAOG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.201"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..864
                     /locus_tag="ACHDLD_002639"
     CDS             <1..864
                     /locus_tag="ACHDLD_002639"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3364302.1"
                     /translation="LSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMNKSYQNLYSERS
                     SSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSLIDKLTKNSITN
                     QDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELNGKK
                     VSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQI
                     LIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNR
                     AKRYGFKQIVE"
     gene            914..1303
                     /locus_tag="ACHDLD_002640"
     CDS             914..1303
                     /locus_tag="ACHDLD_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3364303.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1290..>3618
                     /locus_tag="ACHDLD_002641"
     CDS             1290..>3618
                     /locus_tag="ACHDLD_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3364304.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1192 a          592 c          766 g         1068 t
ORIGIN      
        1 ttatcaaaaa ctgaagagcg attggaagaa attattcaac aagaaataaa gcttaaagat
       61 taccaagttg aggttgactc agcaatgtca agaatgaata aatcatatca aaatctctat
      121 tctgagagaa gtagttcctc gcgcgaaatt ttttctgata ttacaggaat agaggctact
      181 attcaaccaa atagggattt caataattat gttaaagagc tcagagcttg tattaataag
      241 gaggacactt ttgacagtga gtttggatct cttattgata agctgacaaa aaattccatt
      301 actaatcaag atatatataa tgacatactt gatataagag ttagtgggga aagtagtatt
      361 ttttcagata aaaaattaat caattctata agaaaattga atgatgcaca agttcttgaa
      421 ttacaaaccc ttaagccaaa tgatttagta gttatccgac ttgaactaaa tggaaaaaaa
      481 gttagtttat cgaatgctag tgctggtcaa aagacatctg caattctttc tatgattttg
      541 gcgtatggaa attcaccact catattggat cagcctgaag atgatttgga tagtcaatta
      601 attaataact taatcgtaag aagtatcatt acaaaaaagg aaaacagaca aattttaatt
      661 gtaactcata atgcaaatat cccagttaat ggtgattcag aatggttgat tagcatggga
      721 gattcaaaag aaatatcggc tgatcagtgc gggtcggtag atgaacccaa tatcaagcca
      781 agaatatgta ctgtgatgga aggtggcgag gatgctttta caaatagagc gaaacgctat
      841 ggttttaaac aaattgtaga ataacactag agggtgagac atattattat gtccgccctt
      901 tttaggagga aaaatgaaaa aaataaaaag ctataccagt atctgggcag ttgaaaaagt
      961 catttacgca atcaatgatt ttcagttgcc ttttcctgtg acttttaacc aaatggcttg
     1021 gttcgtttta tctttactgt ttgtcatcgt atttgcccat gtgcctccac tatctatgat
     1081 agaggaggtc tttttaaaat acttaggcat accggtggct gtcacttggt ttatgtcgca
     1141 aaagacgttt gatggcaaga aaccactggg atttttacga tcctttatca gctatcactt
     1201 gcgatttaag gtgacttttg caggaaagaa agtcaaagaa cagaagaaac gatgggatga
     1261 acccagtact cttgtaagga gtgtgaacta tgtatccgat taagtatatt gaaaataatc
     1321 tcgtctttaa ccaagaaggc gagtgttttg cctactatga attagtgcct tacaattact
     1381 cctttttgtc acccgaacag aaatttcaag tgcatgataa ctttcgccaa ctaattgcgc
     1441 aaaatcgtga aggcaagatc catgcgttac aaattgccac agaaagtagt attcgcgcca
     1501 cacaagaacg atcaaaaaaa gaaattaccg gtagactcaa agaagtagcg aaacaacgaa
     1561 ttgatataca gacagatgca ttagtatcca tgattggcga tagtcagatt gattatcgct
     1621 tttttattgg ctttaaacta attgcgacag atgaagaagt caatctaaag agtctcaaga
     1681 aatctttctt ttctgggctt caagaatttg tctatggagt gaatcatcat ttgatgggcg
     1741 attttgtttc cttatccaat gaagaaattc gctgctattc taagcttgaa aaattaatgg
     1801 aaagcaaatt ggctcgaaga tttaaagtca ggcgagtcac accgagtgac ctgacgtatt
     1861 tgattgaaca tatttatggc gaaaaaggga taccgtttga agaatacgaa tttcagctac
     1921 caaagaaaaa gctaaaatca gaaacactgg tgaaacgtta tgacttatta cgtccaaatc
     1981 gttgtttaat tgaagaaaaa ccaaggtgct tacggatgga acatgagaat catgagtcgt
     2041 acgtagcgta tttaacaatt aatacaattg tgggggagat ggaatttcct tcctcagaac
     2101 ttttctatta ccagcaacaa caatttacat tcccaatcga cacaagtatg aatgtagaga
     2161 ttgtgacgaa taaaaaggct ttagctaccg ttcgtaataa gaaaaaagaa ctgaaagatt
     2221 tagacaatca tgcttaccaa tctgacaatg aaacgaattc caatgtctta gatgctttgg
     2281 attccgtgga tgaattggaa accaccttgg atcagtcaaa agaatctatg tacaaactga
     2341 gttatgtggt tcgagtgagt gccgaatcag tggatgaact aaagcgacgc tgtgatgaag
     2401 tgctcgattt ttatgatgat accaatgtaa aactagtccg gccctttggc gatatgatgg
     2461 ggctacatga agaattctta ccctcaagta aacgatatat gaacgactat attcagtatg
     2521 ttacgtctga ttttttggcg gggcttggtt ttggggctac tcagatgcta ggagaattag
     2581 aagggattta ctttggctac aatgtggata caggtcgtaa tgtttacctg aaaccggcat
     2641 tggcttcaca aggagtcaag ggttcagtaa ccaatgcgct ggccgccgct ttcctgggtt
     2701 ctcttggtgg aggtaaatcc tttagtaaca atctcttagt ctactatgct gttttgttcg
     2761 gtggtcaagc tgtgattgtc gatccaaaag gggagcgagg agggtggaaa gaaaccttgc
     2821 cagaaattgc ggatgaaatc agtatcctca acttgacgag taagtcagaa aatcaaggtc
     2881 tgcttgatcc ttatgtgatt atgaagaaaa agaaagactc ggaaagttta gcaattgata
     2941 ttctgacttt tttaacagga atttcaagtc gagacgggga gaagtttcct gtgttacgta
     3001 aagccattcg aacggtgact caaagtgatc aacgggggtt attacaagtt attaacgaat
     3061 tgcgaaaaga cgggtcacca atcgcggaaa atatcgccga tcatatcgag tcctttaccg
     3121 attatgattt tgctcatttg cttttctcag acggctcgat tacccaatcg attagtttag
     3181 ataaacagct aaatatcatt caagtggcgg atttggtttt accggatgca gaaacgactt
     3241 ttgaagaata taccaccatg gaattgctta gtgtttcgat gctaatcgtc atttccactt
     3301 ttgccttgga ctttatccat agtaaccgag aaatcttcaa aattgtcgat ttggatgagg
     3361 cttggagttt tcttcaagtg gctcaaggaa aaacgttatc gaataagctt gttcgtgctg
     3421 gtcgggccat gaatgctggg gtctatttcg tcactcaaaa tagcgacgat ttattagatg
     3481 aacgtttaaa aaataatatc gggttgaagt ttgcttttcg cagtaccgat attcatgaaa
     3541 tcaagaaaac cttggaattc tttggtttgg atcaggagga tcaaagcaat caaaaacgcc
     3601 tgcgagattt ggaaaacg
//
LOCUS       DAZAOG010000200         3590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000200 DAZAOG010000000
VERSION     DAZAOG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.202"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            356..1075
                     /locus_tag="ACHDLD_002642"
     CDS             356..1075
                     /locus_tag="ACHDLD_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3364305.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            1077..1919
                     /locus_tag="ACHDLD_002643"
     CDS             1077..1919
                     /locus_tag="ACHDLD_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN3364306.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            1916..2866
                     /locus_tag="ACHDLD_002644"
     CDS             1916..2866
                     /locus_tag="ACHDLD_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN3364307.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            2881..3114
                     /locus_tag="ACHDLD_002645"
     CDS             2881..3114
                     /locus_tag="ACHDLD_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN3364308.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            3445..>3590
                     /locus_tag="ACHDLD_002646"
     CDS             3445..>3590
                     /locus_tag="ACHDLD_002646"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3364309.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQP"
BASE COUNT         1291 a          438 c          664 g         1197 t
ORIGIN      
        1 gaaaaaagtt aaatggagtt tactgatttt catatttgta tatggaacag acatagtctt
       61 gaataatttt tataaatgtt ttgatgctta tcaacttatt caacccgcta tccacttcaa
      121 gtataatttt tccatacgcc aataaagtag ttttaggagc atgttctcat ttgagaacgc
      181 tcctttttta tatattttga aagctactta ctatacgcgt agtttcaaat agttttgtca
      241 ttctaatctt cagcaacaga aaaaaatcta aactataaaa tttagggttg actaaaatat
      301 aatttttgta taccatatga tcgtgataat cattatcatt tagaaggaga aagttatgtt
      361 agaagtgaaa aagcttaatg tttgttataa cgatttttta gcattgaacg agatttcttt
      421 aaaaatacaa gaaggtgctt taacaggtat tattggtcca aatggtgctg gaaaatccac
      481 attgcttaaa gcaatgttaa atattatccc tcatcaggga gaggtctcca ttaataatga
      541 agatatcaat aaaaaactgg tgaagattgc atatgtagag caaaaagctg atattgattt
      601 tacttttcca ataaaagtaa aagaatgtgt atctatggga acatatgccg gaatgaaagt
      661 ttttcaaaga attaaaaatg cagaatggca aagagtttca aaggctttgg aaaaggtcga
      721 tatgggaaaa tattctaatc atcaaatagg agaattatct ggaggacaat ttcaacgtgt
      781 acttttagca agatgtcttg ctcaaaatgc ggatttcata tttttagatg aaccatttgt
      841 agggatagac ttagttagtg aacgaataat tatggatacg ttaaaaaaat tgaaacatca
      901 gggaaaaaca gtgctaattg tgcatcatga tttaagcaag gtaaagaagt attttgataa
      961 tattattctt ttgaatcgaa atctaattgc tcacggaagt gtagaatctg tatttaatga
     1021 agaaaattta aaaaaagcct atggagatac tatatttatt ggagaggggg aataatatgc
     1081 ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct ctaattacat
     1141 ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta agaggaatgt
     1201 ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt tcttatattt
     1261 taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct atcattatta
     1321 cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt actttcagtt
     1381 cttttttagc actaggagtt atccttatag gagttgcaaa tagctcaacg gatttatttc
     1441 atattttatt tggaaatatt ttagctgtac aagatattga taagtggatt acaattggcg
     1501 tttcggtttt agtgttggtt atagtagttc tattttttaa agaattatta atcacttctt
     1561 ttgatccatt aatggcaaaa gcaataggga tgaatgtcag tttttatcat tatctattga
     1621 tggttctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact atattaattg
     1681 ttgcaatgtt aattacacca gcagctacag catatttata tgcaaatagc ttgaaaacta
     1741 tgatatttat ttctgcaaca gtcggagcaa gttcatcatt gcttggattg tttattggtt
     1801 atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc ttatttgtaa
     1861 ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga tcgttatgat
     1921 gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt ttatattcgg
     1981 ggcatgttca ataaatagta aagacaaaga taaagtggct tcgaacgaaa aattaaaagt
     2041 agtagttact aattcgattt tagcagatat taccgaaaat atagcaaaag ataaaattga
     2101 tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt tgcctgaaga
     2161 tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact tggaaactgg
     2221 aggaaatgct tggtttacaa aattagttaa aaatgcgaac aaagaggaaa acaaagacta
     2281 ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg agaaagggaa
     2341 ggaagatcct catgcttggt taaatttaga aaacggtatt atttacgcta aaaatattga
     2401 aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa atctagataa
     2461 gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg cttcaattcc
     2521 gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct cgaaagcgta
     2581 taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa caccagatca
     2641 aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat tcgtagaaag
     2701 cagtgtggat gatagaccaa tgaaaacagt atcaaaagat accaatattc ctatctattc
     2761 aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt actatgcgat
     2821 gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg gagggtaggt
     2881 atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca ggaattatca
     2941 aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt tagtccagca
     3001 tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat aggatttcaa
     3061 ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa gtaaaagact
     3121 ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga cttatcctaa
     3181 tagctggata taattttttt aaatctttct cggattattt tagaaaaggt gaatgattta
     3241 gatgagtcta ttaattcttg taggattgct ttttgtatca gttattctgc tctttctcat
     3301 tagtttagct ctaaggaatg agttcagtaa taaacgttaa cgattgaaac ttcaagtcct
     3361 gtgtaagata ataaagttaa ttcatcttat taggaggaag tacccataga aaccagagat
     3421 tatattataa ctcaccaaaa gttaatagaa agtggactaa gccattttga aaaagaagga
     3481 tttcagcaaa ctaagataag gcaactgtgc ctgaccgcag gcgtaactac gggtgctttt
     3541 tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
//
LOCUS       DAZAOG010000201         3581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000201 DAZAOG010000000
VERSION     DAZAOG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.203"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..878)
                     /locus_tag="ACHDLD_002647"
     CDS             complement(27..878)
                     /locus_tag="ACHDLD_002647"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3364310.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="ACHDLD_002648"
     CDS             1001..1468
                     /locus_tag="ACHDLD_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN3364311.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="ACHDLD_002649"
     CDS             complement(1731..2213)
                     /locus_tag="ACHDLD_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3364312.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="ACHDLD_002650"
     CDS             complement(2313..3176)
                     /locus_tag="ACHDLD_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3364313.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1042 a          771 c          565 g         1203 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa a
//
LOCUS       DAZAOG010000202         3576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000202 DAZAOG010000000
VERSION     DAZAOG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.204"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..128)
                     /locus_tag="ACHDLD_002651"
     CDS             complement(<1..128)
                     /locus_tag="ACHDLD_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN3364314.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSI"
     gene            204..276
                     /locus_tag="ACHDLD_002652"
     tRNA            204..276
                     /locus_tag="ACHDLD_002652"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Lys,seq:ctt)
     gene            438..659
                     /locus_tag="ACHDLD_002653"
     CDS             438..659
                     /locus_tag="ACHDLD_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364315.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(983..1228)
                     /locus_tag="ACHDLD_002654"
     CDS             complement(983..1228)
                     /locus_tag="ACHDLD_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364316.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1222..1623)
                     /locus_tag="ACHDLD_002655"
     CDS             complement(1222..1623)
                     /locus_tag="ACHDLD_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN3364317.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1795..3567)
                     /gene="uidA"
                     /locus_tag="ACHDLD_002656"
     CDS             complement(1795..3567)
                     /gene="uidA"
                     /locus_tag="ACHDLD_002656"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN3364318.1"
                     /translation="MDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVPSSYNDLF
                     ETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQHKGGFTP
                     FESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVNFDFFNYA
                     GINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDEEAQLIAE
                     AKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQVSNGKFLI
                     NNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLA
                     DREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKELIERDKNH
                     PSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDI
                     VDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGLHDT
                     VPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNKKGIFTRD
                     RKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT         1119 a          659 c          481 g         1317 t
ORIGIN      
        1 tctattgatc tatctttcag caaattataa gctttatttt ttccaatatt taacatttta
       61 gttaaatcag taattttcaa tatcgatgga taactttcaa atattttttc ataatcttga
      121 aaatccataa aattcactct caattctgat aataagtatt gcactttttt attttaggtg
      181 ttagaataac aaagtattaa tttgggctgt tagctcagtt ggtagagcag atgactctta
      241 atcatcgggt cgcgggttcg agcccctcac agcccattgg gtgccaaacc cttaaataca
      301 gtgtaaacgt tggtgcttag catttagaaa acgtatatac tgtatttttt tgttttcaaa
      361 agagactgta gtaccccttt ttttcactaa aagcccactg tagtcattga tatttttgat
      421 aagaaaggaa cataattatg cgtaatgaaa ttgctaatat aacaataatc cgagaaactt
      481 tacaaaacca tactgcaaat gagatctcta aacatacagg attaaatttg agtactataa
      541 aaaaactaaa aagtggagaa cgttcaatag aaaaactaaa tttacatgat gctatttgcc
      601 tcactgaatt tggtcttaaa aataatagaa aaaatgttga aatcaacata tggaaataat
      661 tcttaatcag ctgcttttgt caaaagcaca taggaatttc acctctctcc aagtttacgg
      721 cgagccttac ggaagtatca ttatcccaac ttcaaatcat cgctttttat tgactataaa
      781 aattatttta gaatgtttat cgctccttct ttacagtaag aaatcatggc gcaactaaaa
      841 tatgctcctt gaagtgttaa cgtactgata gaatgtattt aaatttcaat gatcaatagg
      901 ttttctgttt aaaagaatct actaatcctt taactattag aagattcttt taaacagaat
      961 aagtggaaag gggacttttt agttagttaa ttttaaattt taagatcgct tctatcaatt
     1021 taatttctag ctgtcttagc atatactcat ctacataggt aataaattgc cccgcctcat
     1081 taaatccatt tgtcaaacat aacttagcta tataactatg ataatgtttg agtatcatct
     1141 tgattgaaat tggatcttct tctagagcag attggataat ggatagagga actaatggat
     1201 actgccgttt tttggggttg tctaccataa gtcatacccc atttttaatt gttcaactgc
     1261 cttctttcgt tgataccaga taccacttgt actcatattg aggattttac cgatttcagt
     1321 atcattaaaa cctgcaaaat aataaagtag gataaccttt cttcttattt ccgataaatg
     1381 atcaatggct gccgataact tttgatctga aattaatatt tccattccaa ataagaaata
     1441 gacatcttgt tcatcaaaat gagtttggga gtacttacct tcatttgaat tcgtcaatgg
     1501 ttcctcatta tctgttatgt taatgtttcg ttttcgaata gacttagttt cgtttcgtaa
     1561 tactttttta cagtaggcat caaattgatg ttgtatagat ttctcattgt aattttgttg
     1621 catgataaat atgctccttt tttccccttt ccactaatac aacaatcaga aagcgccttt
     1681 ttttcgaaaa aactagaaaa aaatatcctt ctttaaacag aaaatctttt tataaaaaaa
     1741 aggagttggc aattaagcag ccactccttt tttatattcc ttataaaagg taaattattt
     1801 tttataactg aaatttggta ttgatgacca tcgtttcttt agatagtgag caatcatttt
     1861 aggctttcta tctctagtaa agattccttt cttatttcct tgcactctta aaatcccttg
     1921 acttgtttca aaatctgcaa aattccacac ttgttcacca acaaaattgt cacatttatc
     1981 tattacttcg tgatttgctt cgtaataacc cacttgatac tcttctgtaa acattgttgg
     2041 tactgtatca tgaagtccag ctactgtatc tgctccatat tctgtaaaca ttattggcgt
     2101 atcgggacat ctttccatcc atccatctag ctcttttttc aacagatatt ttgcatgttt
     2161 taggtctcca cctgaaacat accaaccata atagcgattt aaacataaaa catcgacaat
     2221 atctcctact ttacaaatat ctggtgttga ctttaagtaa gtaacgattg ttacaggtct
     2281 cttctgaggg tcaagttctt tagtcaattc aactaaaggt tcaaaatatt ctttagcgcc
     2341 ttcagaatcc gaatctggtt catttgcaat agaccacatt acgacactcg gatggttctt
     2401 gtcgcgttct atcaattcct taatcgccat tttgtgagca ttttctgtcc gtatttcttt
     2461 ccaagtatca tgtttttcta cgttgtcatc taataaagta gccatgaaat tcaaatgtaa
     2521 tcctactgca ggtacttcat caataactac aataccttct cgatctgcta agcgcataat
     2581 ttcttctgaa taaggataat gtgaagtcct aaatgaatta gcaccgatcc atttcattaa
     2641 attaaaatct ttgatattta ctgcttcatt caaaccacga ccattaatat acgtgtcctc
     2701 atgcttccca aatcccttaa agtaaaaagg tttattatta atcaaaaact tcccattcga
     2761 aacctgaata ctacgaatgc caaattcttc tgtgtacgta tcaatcactt tattttcgtt
     2821 aattaaacta actttcatac tatataagta agcatttaga ggctcccaaa gtgttgggtt
     2881 cttaagttga atcactcctt ctttcccctt agcttctgct ataagttgag cttcttcgtc
     2941 atagattgct attagaattt ctccggtcca ttctgtatcg atattaaatt ctacatttgc
     3001 atctgaatca attatttttg tattaatagt gacatccttt atatagtctt tcggagttgt
     3061 atacaattta acgggtctat taatgcctgc ataattgaaa aagtcaaaat taaccaaatt
     3121 ttcttttttt atgtctccat cttcctgtat cgtttctttc aaaatcccta caggtaaagt
     3181 gctatcgtca ataacattgt ttacagccac tgtaattctg ttacttccat cttttataaa
     3241 ctgattaatt tctgattcaa atggcgtaaa ccctcctttg tgttgattaa tcaattttcc
     3301 attcaaaaat acttttgctt catgtgttgc agatccaaaa cgaagaacta atctttcttc
     3361 taataatttt ttaggaagaa tcacttcttt ttcataccac acccatccaa catgatttct
     3421 aatgttctct gtttcaaata aatcattata agaagaagga actgaaattg gataactctc
     3481 tttttcaacc attggttcta aagcaagttc ttcttgtaag tcatcatttg tatctaactt
     3541 aaatctccaa agaccgctta aatcaattac ttgcct
//
LOCUS       DAZAOG010000203         3571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000203 DAZAOG010000000
VERSION     DAZAOG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.205"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1171)
                     /gene="mnmA"
                     /locus_tag="ACHDLD_002657"
     CDS             complement(50..1171)
                     /gene="mnmA"
                     /locus_tag="ACHDLD_002657"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN3364319.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1575..2174
                     /locus_tag="ACHDLD_002658"
     CDS             1575..2174
                     /locus_tag="ACHDLD_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3364320.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2312..>3571)
                     /gene="efmA"
                     /locus_tag="ACHDLD_002659"
     CDS             complement(2312..>3571)
                     /gene="efmA"
                     /locus_tag="ACHDLD_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN3364321.1"
                     /translation="TNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRETGSATILSF
                     ATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTISESFPIWLV
                     FVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIAPALGAALFS
                     VVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGLQQLMDNKGL
                     WYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLLGGALISFIG
                     TWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPYFNTLLMAMI
                     QQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGIGILLCGIVL
                     FLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT         1089 a          790 c          635 g         1057 t
ORIGIN      
        1 aaggtgtttc ttttgttagc catagctgtt ttgattcatt taatatcgct taaacgtatt
       61 gtaaaacttt tgtttcttga tatgcgtggt cgatcaaacc accacccaga cattccattc
      121 catcataaaa tacaactgct tgtccaggag tgattgcacg tacaggttcg tcgaatacga
      181 cttctgcacg attatcatcc aataagcgaa ctgtcacagg aacgtcttgc tgacgataac
      241 ggaatttcgc tgtacacttg aattcttttg gcattggttc attcgtcgta aagtggattt
      301 cacttgcatc caagcttgtg gcatacaatg caggatgatg gaacccttgc cccacatata
      361 atgtgtttgt cgctagatct tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc
      421 caccgatccc tagtccttga cgttgaccaa tcgtataata catcaagcca gcatgttgtc
      481 ctttgacttc accatcaaga gtgaccatat ttcctttttt tgctggcaga tagttgctca
      541 aaaattgttt gaagttcttt tcaccaataa agcagattcc cgttgaatct tttttcttag
      601 cagtcgcaag gcctgcgcgt tcagcaattg cccgtacttc tgatttttcc atgccgccta
      661 gagggaacat cgtttttgaa agctgttctt gtgataattg gctaaggaag taagtttgat
      721 ctttattatt gtctactcca cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt
      781 gcgcataatg tccagtagct acataatcgg cgccaagatc cattgcataa tccaagaatg
      841 ctttgaattt gatttctttg ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt
      901 attcggctag gaaatattca aatacacgat cccaatattc tttttcaaaa ttcacagagt
      961 aataaggaat cccgatttga gcagctactt tcgctacatc cttataatct tccgtggccg
     1021 tgcatacacc gttttcgtcc gtgtcatccc aatttttcat gaatattccg atcacgtcgt
     1081 atccttgttc ctttaacaaa agtgctgtta cagatgagtc gacaccgccg ctcatcccta
     1141 cgacaacgcg tgttttgctg ttgtctgtca tgatatcacc atcatttcaa ttagattctg
     1201 aaacatctac gagttgaagg tcgcaacttt tcagtatttt acattatacg tcatatttct
     1261 gaaaatttca tcccttaatt atcaagtcgt aaaaatactg ttctactgga cctattcagt
     1321 acacaaacat gatagtcaac caaaaaatca ctcatctttt gagcattcga tataaaaaat
     1381 tggcatactc ataaaaaggg ccagcctttc ggcaataagg aataatttta taaattgtga
     1441 aaaactattt ttcaaaattg cttcttatcg cttaaagctg cacgtctcat ttacaacctt
     1501 ttctttttag atgttatgag ttgctcagat aagtgatttt gtttactcta agaaaaaagg
     1561 gaggcaaaag atacatgaat tactttatca gtgatatgca ctttttccat gaacgcttat
     1621 taggaaaaaa cgactttgct ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt
     1681 tgatcactag ctggaatgca gtaataaaag aaaccgacca tgtttatcat ctaggcgacc
     1741 ttgctatgca tccgcaatat gaaaaagcaa atgcagaaat tttggaactg gtcactcaac
     1801 tgcaaggaac aatccacttg atcaaaggga accatgacag tcgcgctttt ttccgttatt
     1861 tagaaaaaaa tgatcctagg cttactgacg gaagtcccaa attttatttt tatgatgtag
     1921 gtgccatcgt taaattcgac catcaacaat attacttgac tcattatccg atgttgctcg
     1981 ggcctaacga cactatccgc aatcttcatg gacatatcca tcattacagc gtacctatcg
     2041 agaacgatgt caatgtaggc gtagacgcac cggaaagaga actcttaaag gaaaaactac
     2101 catttggcac tccgttatct gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt
     2161 acgataggtc gtgaaaaaac tgccctatat caagtattaa acaaataaag ataattactc
     2221 atatttttca tttgagtagg cttattgcca caggaacatt aggatcagaa atggagttgt
     2281 aacaagactt ctgtcacaac tccattccat actattcgtc tttttgctca tggtgttctt
     2341 ttaccagttt tttctgtaat tctttatcat aatttcgggc agctgacgtt aaaaataaaa
     2401 cgattccgca taataaaatc ccgattccgg aaaacaagaa cattttttct acccctaaac
     2461 gatcagctaa tggtcctgca aataataatc caattggacc tgtcaggttc atcaaagagt
     2521 tgaagttccc taagacacga cctaaaacat tcgattcata actctgctgg atcattgcca
     2581 tcagcagtgt attgaaatat ggcgttgcac aacctgctcc tgcattcaag ataagaaagt
     2641 acaagaaccc ttgactatcg tttggaacca atccgcttgc accgatcgtc aggcccataa
     2701 tgatgtaggc tataataata ggtttcattc ggtctttcca tgttcctata aaactgatca
     2761 gtgcaccgcc taagagcatt ccgactgcgt agactacttc cactgctccc gcctgtccaa
     2821 cggaaccacc aaagtaatct agcgtcatca atggatacag actgattgct ggcatgaaca
     2881 acagcatcac aaatgcacca ttgatcgtta tgtaccataa ccctttatta tccatcaatt
     2941 gctgcagccc aaattttgca tctttaaaaa tagtaagcgg aacttccaat atttcaggag
     3001 aaacttttgg gatcttcaca aaaatcaata agccgacacc aaaaactgcc cccaatacat
     3061 ctaataaaat aaggtaattg acaggaacga ctgaaaacaa agccgcacct agagctggcg
     3121 caatgatgaa attcgctgat tggaccattc ctaactgtcc attcgtcctt gttatgtgag
     3181 aagatggaac gattgtcggc ataatcgact ggatcgttgg catttggaat gtttgtgcga
     3241 ccgaacgcat aaacaaagac acaaaaacta gccaaatcgg gaaggattct gaaatcgttc
     3301 cgactactgc taaaataagc gcgaaaatcg ctacaataat atccgtgaca atcaaaagag
     3361 cttttttatc ccagcgatcc actagcggtc ctacaaatgg gcttagtaaa accatgggaa
     3421 tcattcctag taaagtagca aaactcaaaa ttgtcgccga accggtttct cttgtcaaat
     3481 accagatgat cgcatattgg acgaccatac tagtgattcc tgacaaaaac tgtcccgata
     3541 aaaagaacaa ataatttctc ttccaattcg t
//
LOCUS       DAZAOG010000204         3539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000204 DAZAOG010000000
VERSION     DAZAOG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.207"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..362)
                     /locus_tag="ACHDLD_002660"
     CDS             complement(<1..362)
                     /locus_tag="ACHDLD_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFN3364322.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIE"
     gene            415..891
                     /locus_tag="ACHDLD_002661"
     CDS             415..891
                     /locus_tag="ACHDLD_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364323.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(897..1079)
                     /locus_tag="ACHDLD_002662"
     CDS             complement(897..1079)
                     /locus_tag="ACHDLD_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364324.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(1079..1378)
                     /locus_tag="ACHDLD_002663"
     CDS             complement(1079..1378)
                     /locus_tag="ACHDLD_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364325.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(1483..1686)
                     /locus_tag="ACHDLD_002664"
     CDS             complement(1483..1686)
                     /locus_tag="ACHDLD_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364326.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1701..2447)
                     /locus_tag="ACHDLD_002665"
     CDS             complement(1701..2447)
                     /locus_tag="ACHDLD_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN3364327.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2525..2833
                     /locus_tag="ACHDLD_002666"
     CDS             2525..2833
                     /locus_tag="ACHDLD_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364328.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2804..3061)
                     /locus_tag="ACHDLD_002667"
     CDS             complement(2804..3061)
                     /locus_tag="ACHDLD_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFN3364329.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(3359..3499)
                     /locus_tag="ACHDLD_002668"
     CDS             complement(3359..3499)
                     /locus_tag="ACHDLD_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364330.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT          976 a          686 c          567 g         1310 t
ORIGIN      
        1 tcttcaattg tttgaggaac agtattcttc cgaataacat cttccatttc gttgaaagct
       61 tcaataaatt ttagtttgaa atttattgct ttacttccag tgaatcccat agctagcaag
      121 gaaaatccgt ctctattcat gaaataaact ctccgacttc ttccgtatga gtctggctcg
      181 ttcccttcca caaacatctg tccaaaattg gacccatctt caacattggc cgaattttcg
      241 accgatcttt ttattgattc aattgcttct agtacatgct tatgtttctt tttgaaactt
      301 tctgccactt gtaagctcgt agttacagct tctttatttt tcaaaattac taattcttgc
      361 attatttctt ctctcctttt tggtataatt ttcgttagaa agtgaggtga ttctatgagg
      421 aaaaagctca attacattct tactgcgact ttaaccatat atataattct gtttattacc
      481 ttatctttcc tgtcttcaat taaaaaaatt ccttttatta cattgaataa aaatagtatt
      541 ttgaaggagt tcaataacac tgactacatc gcatttctaa aacttttttc agtctgtgtg
      601 ctaatatcat gtgtaattat ttttattgtt gctaaaacat gttggacaat gttaaataaa
      661 aaccaacgcg atgttgaaaa tattgctata tttttaatta agattacttc ctcaatatca
      721 attatcattc cgatgactgt aactttttca aaagaacagt ttaatgtagc ggctacttat
      781 ctttcattta tagcgctatt ttcttttgtt cttcctaaaa gaaataagag caactttgac
      841 aacaaggagc tggatgaagt acgcaatagc agcccaaagg aacaaaaata aaaaaatcat
      901 attttagtca gtccctcccg actggctttt ttcgctctgt gctcagcttc atcaagcccc
      961 ataaaaatcc aaaccatgta aacgattgtc cctatcaacg cttgtctgct tccccagagg
     1021 cctaaagcgt agatgattag cggtgcgctg aatactagtg ctctgttgaa tttgcccatt
     1081 aacttgcctc cttcaaaaca tctgaaggcg ataaactttt tacacgatat ttgtttgcgt
     1141 ctttccactt taaaaaccaa ataaaagtat ggtaatgaat aaatgtggtt gagtgacctg
     1201 gacgaagtat tccttctgca aattcaggaa ttgtttccat ttccttgcag tattcaacta
     1261 aagtagtttt tgacatgccg tggaacttct ccaagattaa actttgtcgg taccagtcgt
     1321 ctggttttac tgccttctca gcagcttcta tcagctctga aagtgttggt tttttcactt
     1381 attatccctc ctaaaaatta tcgttccata ctcgatgttt aacatttctt gaagctctga
     1441 ttttgtaaga atgattttat cttccaacga ctttacctcc ctctacaatt cgtacatagt
     1501 gataatcgaa tctataattc tgtttgcttc tgcagaagtc tttttaccgt ttaaaattaa
     1561 agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt
     1621 tgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc
     1681 agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatagccc
     1741 tctgcgatta ataagttgta aataaacact cgccctttct gtgtccattt ggtattcatt
     1801 acaactttag tgccgccatc ggctttcggt atctcacttg tgtgcgattt tgtgtatcct
     1861 tggttcatat gttttcggta aagtatccat tgtccgctaa ctttatgctg aacacctaac
     1921 tcgtttagca atttgtttag agcaattgcc gacattccgt aatcagctgc aatttgagaa
     1981 gtagctaccg tatctgtcga tgaaagaatc gtatctaagt agctaatctt tggttcgtac
     2041 tcggcaattt gttgttcgag taattggttc ttttcttcta aatccgctgc taaacgcaat
     2101 gcttgtgcaa aactttgagg tacgtttgaa tagctacctg tttttctaat tgttggaagg
     2161 acttcactcg ttacccaacg tttaaatttt ttggcagagg gaagttttga ttttaagatt
     2221 aaactgtaaa ggcctgactc gttaatcaaa acaactgctg ttccattgac ggtgaacgaa
     2281 tcgttctgcg tcttatcctc caagtccaca tgatctcgaa tagctttttg cggattcgaa
     2341 tatcctagaa cgtctgctac atcttttcct acaaaatatg gttcatcatt tactaaaata
     2401 gttcgaactt cgttttgctc gaaattaaaa atttgtggtg tattcatatt tctcattcct
     2461 ttctttggta taatttcctt atcagtgtga caggctgaaa taattgttaa ggaggtgaaa
     2521 atttatgaaa ccagaatttg taattactat gcttgatgga gaaaaaatca aaatatttgc
     2581 cgatactttg gtcgttggat acgatgatgc gtcaaatatc gaagaaaaag atggaatgtt
     2641 ctattcttct caaatttacg tgaatacctt gcaaggtgat attggggtat ctactcttgc
     2701 aacatcaaga ccgcttgtag gtcttatcgg ctttttgtca aaagcaacat tcttttctct
     2761 cggcgatgat atagattcgg atgaaactgt catctataaa acatcagctg ttaaaagtat
     2821 cggtttcaaa tagtaattct tgctcgttga tagcggaagc caacttttct tccaacacat
     2881 cggcgagctt tctttttgtt ggattcattt caaagttgat agtcttgaga agcatttttg
     2941 catccttaac tttttgtcct tgcaattcct tgatgatttt atcggcttgg ctatcaattc
     3001 catgtgttag atcatctatc attttttgta ccttgattgt ttggctcatt tccttgttca
     3061 ttgtttagcc tcctttaata aatgattttt tgtatttttt aatttccata aattcctcaa
     3121 attgctcttt ggtattatta ccacgaccat acatattgtg aaattcttta tggcaactga
     3181 cacaaagtgt aacgccgttt gaaatcgctg cggatttagt tgttgcatgg ctaaatcacg
     3241 aatcacaagt atctactaaa ggcgaatcaa tttcgccaaa agaaattgtt caagcttatt
     3301 tagatattca ttatgctgtt atctatggtc agcttccaga agaccgtaaa aacgatgact
     3361 aaacgtgagc taaaatctct gctatggctg caaccatggc agagtcttct ttatctaccg
     3421 cgtgttccat cgccactttt gcttgtttca gtatgtctaa tttcaaatct tcgatttttg
     3481 ccgagactgt tttttccata ttcttctctc ctttctttta tattcgtaaa caaatttaa
//
LOCUS       DAZAOG010000205         3470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000205 DAZAOG010000000
VERSION     DAZAOG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.208"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACHDLD_002669"
     CDS             <1..909
                     /locus_tag="ACHDLD_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364331.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
     gene            923..1072
                     /locus_tag="ACHDLD_002670"
     CDS             923..1072
                     /locus_tag="ACHDLD_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364332.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ACHDLD_002671"
     CDS             1069..1515
                     /locus_tag="ACHDLD_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364333.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ACHDLD_002672"
     CDS             1517..1654
                     /locus_tag="ACHDLD_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN3364334.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ACHDLD_002673"
     CDS             1691..1984
                     /locus_tag="ACHDLD_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364335.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ACHDLD_002674"
     CDS             1981..2205
                     /locus_tag="ACHDLD_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN3364336.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ACHDLD_002675"
     CDS             2202..3221
                     /locus_tag="ACHDLD_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3364337.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1199 a          621 c          725 g          925 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt
//
LOCUS       DAZAOG010000206         3458 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000206 DAZAOG010000000
VERSION     DAZAOG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.209"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="ACHDLD_002676"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="ACHDLD_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN3364338.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="ACHDLD_002677"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="ACHDLD_002677"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN3364339.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..3432
                     /locus_tag="ACHDLD_002678"
     CDS             3229..3432
                     /locus_tag="ACHDLD_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN3364340.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1097 a          583 c          813 g          965 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3421 gaaaaaaaat aaaaaccact tattactaat tgaatcaa
//
LOCUS       DAZAOG010000207         3453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000207 DAZAOG010000000
VERSION     DAZAOG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.210"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACHDLD_002679"
     CDS             complement(<1..1097)
                     /locus_tag="ACHDLD_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3364341.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACHDLD_002680"
     CDS             complement(1227..2603)
                     /locus_tag="ACHDLD_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3364342.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACHDLD_002681"
     CDS             complement(2603..3259)
                     /locus_tag="ACHDLD_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3364343.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>3453)
                     /locus_tag="ACHDLD_002682"
     CDS             complement(3269..>3453)
                     /locus_tag="ACHDLD_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3364344.1"
                     /translation="NSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFAS
                     SLMLIKKPKEILSKIS"
BASE COUNT         1064 a          641 c          461 g         1287 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taa
//
LOCUS       DAZAOG010000208         3446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000208 DAZAOG010000000
VERSION     DAZAOG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.211"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHDLD_002683"
     CDS             complement(220..972)
                     /locus_tag="ACHDLD_002683"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN3364345.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHDLD_002684"
     CDS             1085..2005
                     /locus_tag="ACHDLD_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3364346.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHDLD_002685"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHDLD_002685"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN3364347.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
BASE COUNT         1203 a          576 c          687 g          980 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaa
//
LOCUS       DAZAOG010000209         3446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000209 DAZAOG010000000
VERSION     DAZAOG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.212"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(766..2220)
                     /locus_tag="ACHDLD_002686"
     CDS             complement(766..2220)
                     /locus_tag="ACHDLD_002686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364348.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2221..2520)
                     /locus_tag="ACHDLD_002687"
     CDS             complement(2221..2520)
                     /locus_tag="ACHDLD_002687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364349.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2823..3194)
                     /locus_tag="ACHDLD_002688"
     CDS             complement(2823..3194)
                     /locus_tag="ACHDLD_002688"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN3364350.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         1059 a          736 c          556 g         1095 t
ORIGIN      
        1 taaagttctg tcaaatatag tcactttata ttactttctc ttgagtaaaa aaacatgtga
       61 ggtcgtaaac aaagattgaa ctatctacat tcatactcta taatctgttt atttgcagac
      121 tgataatagc aaaagaaaac ttataatcaa ataatatcaa catttgatca cataatttga
      181 gattgaatgc tgatttacct atgttttacg taatggtctt tagttatttt tgctcataaa
      241 atgcataaaa cacgcgtttt tatgtgtgct tgaaatttct tggctctata atttctaaga
      301 ctgataaaaa tattctatcg gttctttttg caaataaaaa aacatataga agctccatta
      361 gattgaatca ccacaacaca atcaaaggag cattccgtat gcttgatttt cattctataa
      421 aactactttt aggaatcaaa gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga
      481 agtaccattg tttcatcgat catgagaaaa gcattcacct ctgtctgcag atgttcttat
      541 atcgctctgt cagtcctatt aataaagtac tgttttgttt tcaaaaaaac tattacgagt
      601 actttttaag agaatgcctt gcaaatattc atcacacacc gttatataag tcaaatagtt
      661 tctttcagtc gtcaagaaaa gattctcctg tccttggcat tatcagttaa gtagcaactt
      721 aattctcgga cttgttctac tctagcaaaa tttattttca tcaacttacg tccaaatgtt
      781 tttttgaata attacttcat cgcgggtctg gaagacttgc ctcattcgat aagtcactag
      841 cttttggcgt ccttcaggat ttctagtata caccaactga catttgggca gcgacgattg
      901 atttaattcg tttagaaaaa ggttagcgat ttgcttattt cggccaaagc gttctggtac
      961 cgtcagaact atctttttcc cttcgattaa ataccgcgga ttctccagcg gtgctaaaag
     1021 ctccctcata gcttcagtaa aaagctgtcc ttctcgatag ccagcattct caagatagca
     1081 tctaaattcc tgctgatcag ccgctacgat tacttttcta tcactaactt caccaaggcg
     1141 ctgcaacgtc gtttgaacgc atatggccaa gctttttttg cgggcacatt ccttttccca
     1201 tagcttttgt cgcctacgaa tttggaaata ttccagcaag ttacctttaa atatagctga
     1261 ccagaagctt agaggctcta tattttttac tactatcggc aatgaagttt cttgtttttg
     1321 ctttacctga tcgactttcc taacgacgat tggcatagat aggtgccagc catcagctaa
     1381 agcagtactc cattgttctt tcaaccgact tcgttcacaa gaaaaataga acatcgactg
     1441 agtataagtt tccatcgtgg tggtcgtata ttccagtggt aaagcaaagc gatcgccacc
     1501 actctcaatt tttggtgaag attcaaaggt cagacctgaa atattgccca aacgccgtag
     1561 cattcttttc aaatcaggct gttcagacag aggaactccc ggtaatccaa tcgctaaatg
     1621 ccaaatgttc gttactttat tttgtgcacc taggcgtaag ccccgccctc gtagttgttg
     1681 tgtttgcaca aaagtggaag cttgattagc caagatcaac agattaattg caggacaatc
     1741 ccagccttct cctagcaaac taacggttcc taccaaaata ttcaacttct tttcggttaa
     1801 taggcgagta actgcttgca tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa
     1861 ataacccgac atataaggat gttcattcat acccgtcgct agttctgcca gctcaacagt
     1921 tactagcatt ttcggtaaca gtaagaactc tccacagatg gcaccgattt gctgagtgtt
     1981 acctaataga cgaacaactt catcaaacaa aggaaccacg ccataagcta aggtaccatc
     2041 actgcctgct aatacctcct tgcaaatacg atccatcaaa atgaccgctg tcagttcttg
     2101 cttcagctga acttcttctt ttaacaaaat ttgacaaact gcttgctttt tctcaggaat
     2161 cgcctgacta ccaacagtgt ctggatactc tggaaacaaa tgcagcgact catcaaacaa
     2221 tcatccagct tttttcaatt gataaaaaat aggcaattcc ggttgatctt caaacatctg
     2281 ctgataaata gtgatcagct cttttttacc taaagatgga actgaagctt ttttaccttg
     2341 ctgcttagaa ctgcctaata gctgccaatg ttcgggtatc aattcatagt tatgatgatt
     2401 caagtaaatt aatgcactag tgaacagctc aaaattttca taaatttcct tcgaatgctc
     2461 aagcggatgc tgaacaaatt ctaaagaaag caaataatcc accgtctctt ggctttccat
     2521 taacgcttcc accagctcat tttgtttctc taaaaaggta aaataatgct gagtagtttc
     2581 ctgattagcc ggaatcaaat atagaaaatc ctgatgagga catagaacct cctcctttac
     2641 caactcggga atgctaattt cttcgtcaat caggccattt taactcaata tattgcttcc
     2701 actccgtctg agagctatca atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg
     2761 gttgtttttt cttcaagtca atcaacaatt caccccagct tttttttaaa tgatgagctt
     2821 catcaaaaaa ctaacagctc cagctgatca ccataagttg gctcctcctt cagtcggttg
     2881 taaatggtct gatacgtttc cactgtaata tgactaggat gttcaatcgc cccagaaaga
     2941 tagcaactat ctgctggatt tactaagaaa tattcctcga ttgttgttaa ccactgtttc
     3001 ttcaatatta gggaaggaac caaaattaag gttttacaat ccaaacgtcg gatcatctca
     3061 attcctaaaa aggtcttgcc agcccctggt ggagccacta gatggactga attgttattt
     3121 aaataagtag caaagttagt caatatacgc ttctgatagc tacgccactg aattttttca
     3181 tcagatagcc acatcccagg tccccctctc acgtattttc tctatcataa tctaaatgaa
     3241 tacagcaaaa aaaagatttc ttcaagaata tgtgttttta tagtaatatt ttacttactc
     3301 taaactcaaa tgaaaaggta aaaaaactgg tagtacaatc ttttaaattc tcattgtttt
     3361 gtacgttctt tactatcaaa aaaacaaaaa aaacgcgttt ctacgcgtga ccaaaatttt
     3421 tagctctata atttctaaga ctgata
//
LOCUS       DAZAOG010000210         3403 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000210 DAZAOG010000000
VERSION     DAZAOG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.213"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..234)
                     /locus_tag="ACHDLD_002689"
     CDS             complement(<1..234)
                     /locus_tag="ACHDLD_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364351.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSS"
     gene            complement(532..1506)
                     /gene="sgrA"
                     /locus_tag="ACHDLD_002690"
     CDS             complement(532..1506)
                     /gene="sgrA"
                     /locus_tag="ACHDLD_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN3364352.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1702..2541)
                     /locus_tag="ACHDLD_002691"
     CDS             complement(1702..2541)
                     /locus_tag="ACHDLD_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN3364353.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2726..>3403
                     /locus_tag="ACHDLD_002692"
     CDS             2726..>3403
                     /locus_tag="ACHDLD_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN3364354.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDP"
BASE COUNT         1108 a          711 c          502 g         1082 t
ORIGIN      
        1 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
       61 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
      121 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
      181 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
      241 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
      301 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
      361 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
      421 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
      481 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
      541 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
      601 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
      661 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
      721 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
      781 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
      841 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
      901 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
      961 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     1021 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     1081 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     1141 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     1201 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     1261 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     1321 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     1381 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     1441 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     1501 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     1561 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     1621 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     1681 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     1741 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     1801 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     1861 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     1921 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     1981 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     2041 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     2101 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     2161 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     2221 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     2281 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     2341 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     2401 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     2461 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     2521 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     2581 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     2641 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     2701 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     2761 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     2821 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     2881 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     2941 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     3001 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     3061 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     3121 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     3181 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gggaaaattt
     3241 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     3301 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     3361 aaaaaacggg gaagtttctg atatcattcc tgtaacagat cca
//
LOCUS       DAZAOG010000211         3398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000211 DAZAOG010000000
VERSION     DAZAOG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.214"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2175
                     /locus_tag="ACHDLD_002693"
     CDS             <1..2175
                     /locus_tag="ACHDLD_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFN3364355.1"
                     /translation="LQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALRILLDD
                     SYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSESQTI
                     LEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYYT
                     SKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALR
                     DVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINR
                     QLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKI
                     VEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVS
                     EISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSK
                     GVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCA
                     IVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDV
                     NKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIEL
                     GEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTS
                     DTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2172..>3398
                     /locus_tag="ACHDLD_002694"
     CDS             2172..>3398
                     /locus_tag="ACHDLD_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN3364356.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1285 a          457 c          571 g         1085 t
ORIGIN      
        1 cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga taatgagaca
       61 aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc attgagaatc
      121 ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga tttttttcat
      181 ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt tgaaggaatc
      241 agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc tgaaagtcaa
      301 acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat ttctctattt
      361 attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga attagactct
      421 catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt ttactacaca
      481 tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt aggaagactc
      541 aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg ctcaagaatt
      601 gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct tcgagatgtt
      661 cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac aattgaatca
      721 aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt aaatgagact
      781 attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca actaaatagc
      841 ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc agatgatatg
      901 ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt agacttacta
      961 gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt tgaagcgtgt
     1021 cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca tattcatcat
     1081 catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac tcagattctc
     1141 atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat gaatgtatta
     1201 aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa ctcatttgct
     1261 aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag atatttagat
     1321 gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg agatgctgaa
     1381 gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga cgaacttgga
     1441 ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt gtttgacgat
     1501 gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc aatcccatca
     1561 gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa agaaaaatta
     1621 tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac aacatttgaa
     1681 attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc aattatagat
     1741 gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca aagtgaacaa
     1801 tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa ggggtggttt
     1861 gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta tattcttaat
     1921 gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa aattctactc
     1981 catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc tagtttaaaa
     2041 acaagtgata cagctgattt gaatatagca ataatttctt tattagagta ctttggtaaa
     2101 gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat actttatgct
     2161 gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat ttagaacaaa
     2221 aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg agtgggaaaa
     2281 caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga tcactaaaaa
     2341 gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa cgcctaagtt
     2401 tacttagtat agatgatgat attaatattt gggtggggac aatacatcag ttttgtcttg
     2461 aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga tttacaatta
     2521 ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg aatttaaatc
     2581 tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt aaaattaatg
     2641 aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa aataaagaaa
     2701 ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac agtaacgttg
     2761 cacgtaatat tgctagtaat ataagggcga tttatgtgga tgaatttcaa gatactagag
     2821 aattacaata caatgtaata gctaaattac ttcaaaacaa ccctcaaatt cagtcaatgt
     2881 ttgttggaga tattgaccaa gcaatttatg gatcattaga tggtatagct aaatcagttg
     2941 aagaactaga aatgctaact agtcacactt ttcaatcaaa gacgctacat ggctgttaca
     3001 ggtctaatca gagattagta gacttctact ctaattttca gagttgtcct tacgagattg
     3061 aaagcagagg gaataataaa aacgaccgtg gttttataag ttatgattgc aaagttacta
     3121 aggaggattt acccaacata ataaaaaata taataaagga aaagattgaa agcggcattc
     3181 atgaaaaaga tatttgtgta cttgctcctc aacactatcc tctgttttca ctgggtgagt
     3241 atttaaagaa agaattaccg tattgcaatt ttgatgctat aaatatatca ccaattaagg
     3301 taaataatca tagtttattt ttcaagttag caattttgta ctttactgaa tcaggtgaaa
     3361 aggttcgaag aagaaagcta attgcaaacg atatttta
//
LOCUS       DAZAOG010000212         3371 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000212 DAZAOG010000000
VERSION     DAZAOG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.215"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1013
                     /gene="rpsB"
                     /locus_tag="ACHDLD_002695"
     CDS             231..1013
                     /gene="rpsB"
                     /locus_tag="ACHDLD_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN3364357.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1112..1993
                     /gene="tsf"
                     /locus_tag="ACHDLD_002696"
     CDS             1112..1993
                     /gene="tsf"
                     /locus_tag="ACHDLD_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN3364358.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2129..2851
                     /gene="pyrH"
                     /locus_tag="ACHDLD_002697"
     CDS             2129..2851
                     /gene="pyrH"
                     /locus_tag="ACHDLD_002697"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN3364359.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2854..>3371
                     /gene="frr"
                     /locus_tag="ACHDLD_002698"
     CDS             2854..>3371
                     /gene="frr"
                     /locus_tag="ACHDLD_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN3364360.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVD"
BASE COUNT         1227 a          592 c          752 g          800 t
ORIGIN      
        1 ctactgtttt tcttgaattc tttttatctt tcaggtaaac tattgaatgt tgagcagaaa
       61 agtatatttg ctcttgcgat acggatcttt tgtctgtata atagctgagg gtgctttatg
      121 catcagcaaa tccacattct tattggattg tcggtaggtg cttgaaaaag tttgccggca
      181 aggtagaaga taagaagagg aaaaataaaa ccaaatcgga ggaaacaaac atggcagtaa
      241 tttctatgaa acaattacta gaagccggcg tacactttgg tcaccaaact cgtcgctgga
      301 atccaaaaat gaagaaatat atcttcacag aaagaaacgg aatctacatc attgacttgc
      361 aaaaaacagt taaattagta gatgcagctt atgattacat gaagagcgtt gctgaagaag
      421 gcggcgtagc attattcgta ggtacaaaaa aacaagcaca agaagcaatc aaagaagaag
      481 caactcgtgc aggacaatac ttcgttaacc atcgttggtt aggcggaaca ttgacaaact
      541 gggatactat ccaaaaacgt atcagccgtt tgaaacaaat caatgcaatg gaagaagatg
      601 gaacttttga agttctacct aaaaaagaag ttgctggttt gaacaaagaa cgtgaacgtc
      661 ttgaaaaatt cttaggcggt atcgctgata tgccaagaat cccagatgta atgtacatcg
      721 ttgacccacg taaagaacgt attgctgttc aagaagcaca caaattaaat atcccaatcg
      781 tagctatggt tgatacaaac tgcgatccag atgaaatcga cgtagtaatc ccttcaaacg
      841 acgatgcaat tcgtgccgtt aaattgatta cttcaaaaat ggctgatgct ttcatcgaag
      901 gaaaccaagg tgaagatcaa gtagttgaag aagatttcac agaagaaaac acagcaacat
      961 caatcgaaga aatcgtagat gttgttgaag gcgacaattc ttcagcagaa taattttgaa
     1021 tgatggagct gtttcaaagg ctgggcgaca agcctcactc tcctttgaag cagctttttt
     1081 taaaaagaaa aacacacagg aggagttaaa catggcagac gttacagcta aaatggtaaa
     1141 agaactacgc gaaatgactg gcgtaggaat gatggatgcg aaaaaagcat tagttgaagt
     1201 agaaggcgac atggaaaaag cagttgatct tttacgtgaa aaaggaatgg caaaggctgc
     1261 gaagaaaaat gaccgcattg cagctgaagg attggcttca gttgctgtga aaggtaacac
     1321 tgcagcaatc gttgaagtaa actcagaaac agactttgtt tctaaaaacg aaatgttcca
     1381 agatttagta aaagaaattg cagaacttgt tgctgaaaac aaaccagcag acatggaagc
     1441 tgcaatgaaa atcaaaacag ataaaggtac aattgaatct gacttgatcg aagcaactca
     1501 agttatcgga gaaaaaatca gcttccgtcg ctttgaagtt gtagaaaaag acgacaatgc
     1561 tgcatttggt ggatacttgc atatgggcgg acgtattgcc gtattgactg ttctagatgg
     1621 aacaactgac gaaacagttg caagagacgt agctatgcac gtagcagcta tcaatcctcg
     1681 ttatgtaaac gaatcacaaa ttccagaagc tgaattagaa catgaaaaaa ctgttcttac
     1741 agaacaagca ttgaacgaag gcaaaccagc gaatatcgtt gaaaaaatgg tagaaggacg
     1801 cttgaagaaa ttcaaagcag aaatcgcttt ggtggaccaa ccattcgtta aagatcctga
     1861 tatgactgtt gaaaaatacg ttgcatcaaa aggcgcaact gttaaaacat tcgtacgttt
     1921 cgaagttggc gaaggtatcg aaaaacgcga agataacttt gtagaagaag ttatgagcca
     1981 agtgaaaaaa taattctgtt gaataacaga cgggaacgca cggtttgtgc gctccctttt
     2041 tttaagcaaa acaagaaaaa ccagtcttag agtgtcgaaa tcatctaagt atggtaaaat
     2101 ggcaatagaa aatattggag gaatgaacat ggtcacaccg aaatatcagc gtgtagtctt
     2161 aaaattgagt ggagaagctt tagctgggga cgcaggattt ggaatcaaac caccagtaat
     2221 caaagaaatc atccaagaaa tcaaagaagt tcacgagctg ggcgttgaaa tggcaatcgt
     2281 tgtgggcggc ggaaatatct ggcgcggaca aatcggagca caaatgggca tggaacgtgc
     2341 tcaagctgat tacatgggta tgttagctac agttatgaat gccttagctt tacaggatac
     2401 gttggaaaac gtgggtgtac cgacacgcgt gcaaacttcg attgaaatgc gtcaaatcgc
     2461 agagccatat attcgccgta aagcagaacg tcatttagaa aaaggacgga tcgttatctt
     2521 tgcaggcggt accggcaacc cgtacttctc gacagacaca acagcagcat tacgcgcggc
     2581 agaaatcggc gcagatgtta tcctgatggc taaaaacaat gtagacggtg tttattctgc
     2641 tgatccgaaa gtagatgcaa atgcagtaaa atttgaagaa ctgactcatc tggaagttat
     2701 tgccaaagga ttacaagtca tggattcaac tgcaagttca ctaagcatgg ataatgatat
     2761 tccactgctt gtcttcaatt taaatgaaca cggaaatatt cgccgtgcaa tcttaggcga
     2821 aaatatcgga acaactgtaa gggggaaata aaaatggcag atgcaattat gacagaagca
     2881 aaagaaaaaa tgaaaaaagc agcacaaaat cttcaacgtg aacttgggca aatccgcgca
     2941 ggccgtgcga acgctagcct tttggaccgt attactgtga actactacgg tgcgccaaca
     3001 ccattgaacc aaatggcatc aatccaaatt ccagaagcac gtgttttaat gatcacacct
     3061 tttgacaaga gcattcttca agatgttgaa aaagctatta tggcaagtga tatcggaatc
     3121 agtccaacca atgatggaaa tgtcattcgc ttagttatcc cgcaactgac agaagaacgc
     3181 agaaaagaat tagctaaaga tgttaaaaaa gaagcagaaa atgcaaaaat cgcgattcgt
     3241 aacatccgtc gtgatgcgat cgatgaatat aaaaaacaac aaaaaaatgg tgatattacc
     3301 gaagatgatt tgcgcggatt agaaaaagac gtacaaacat tgacagataa cagcatcaaa
     3361 gaagtagata a
//
LOCUS       DAZAOG010000213         3325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000213 DAZAOG010000000
VERSION     DAZAOG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.216"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHDLD_002699"
     CDS             complement(<1..2232)
                     /locus_tag="ACHDLD_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3364361.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHDLD_002700"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHDLD_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          913 a          744 c          535 g         1133 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaatt
//
LOCUS       DAZAOG010000214         3291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000214 DAZAOG010000000
VERSION     DAZAOG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.217"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..319
                     /locus_tag="ACHDLD_002701"
     CDS             <1..319
                     /locus_tag="ACHDLD_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN3364362.1"
                     /translation="KECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINAN
                     LNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQ
                     IAY"
     gene            292..969
                     /locus_tag="ACHDLD_002702"
     CDS             292..969
                     /locus_tag="ACHDLD_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN3364363.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            920..2101
                     /locus_tag="ACHDLD_002703"
     CDS             920..2101
                     /locus_tag="ACHDLD_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN3364364.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2114..3091
                     /locus_tag="ACHDLD_002704"
     CDS             2114..3091
                     /locus_tag="ACHDLD_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3364365.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3091..>3291
                     /locus_tag="ACHDLD_002705"
     CDS             3091..>3291
                     /locus_tag="ACHDLD_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3364366.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITN"
BASE COUNT         1235 a          406 c          631 g         1019 t
ORIGIN      
        1 aaaagagtgc aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt
       61 caaaatacca gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat
      121 tataaatgca aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc
      181 tcagtttata aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag
      241 aagaataaca aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca
      301 aaaacaaata gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga
      361 cacgcaaaag tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt
      421 gatgataagc aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat
      481 agctgggcgg atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta
      541 gtaagaaaaa agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt
      601 catccaagtg ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg
      661 aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc
      721 attgttgagc atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta
      781 gcgggtggag tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag
      841 atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt
      901 ccctcacatt ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt
      961 gaattctaga attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc
     1021 taaggctttt aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga
     1081 aagtattaca aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc
     1141 tgccatacat cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc
     1201 ttcatttact tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt
     1261 cattgactca gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga
     1321 agaagctgaa agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca
     1381 aatggctgat tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga
     1441 agatgcagcg gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac
     1501 aattgggatc tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct
     1561 agtgtctagt gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga
     1621 tcaagcaccg tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc
     1681 tgccggtatc ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga
     1741 aattttcaat cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga
     1801 agtggatggc atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc
     1861 tattgatcaa gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc
     1921 tttgtggaaa ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga
     1981 taataatctt agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag
     2041 taatatgaca gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta
     2101 ggtgtgatat tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact
     2161 tagactcatt caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc
     2221 gagacgaaag gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc
     2281 aaaaaatttg tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt
     2341 tattataaaa gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa
     2401 tgaatattat gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc
     2461 ttctacattt ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa
     2521 aaaaatttct tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga
     2581 ttatcgcaat ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat
     2641 tgaattacct tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca
     2701 gaaattatcc aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga
     2761 aactggtata gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt
     2821 aactataatt ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc
     2881 atggaatgaa gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga
     2941 taataattat tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt
     3001 cacgcttttg gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat
     3061 taacttaatt aacgacattg aggtaaatta atgatgaaag taattttttt tgggttaggg
     3121 tcaattggaa agagacatct aaaaaatcta ataagctatg gaaaaaagaa ttctattaat
     3181 tttgatataa cagcttatag tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt
     3241 caaaatagag acgatttgga tacacattat gatatagctt ttattacaaa t
//
LOCUS       DAZAOG010000215         3272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000215 DAZAOG010000000
VERSION     DAZAOG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.218"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            588..1004
                     /locus_tag="ACHDLD_002706"
     CDS             588..1004
                     /locus_tag="ACHDLD_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN3364367.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="ACHDLD_002707"
     CDS             1018..1584
                     /locus_tag="ACHDLD_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN3364368.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="ACHDLD_002708"
     CDS             1607..2593
                     /locus_tag="ACHDLD_002708"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN3364369.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3272)
                     /gene="scm"
                     /locus_tag="ACHDLD_002709"
     CDS             complement(2748..>3272)
                     /gene="scm"
                     /locus_tag="ACHDLD_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN3364370.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT          980 a          612 c          698 g          982 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgctatcact tgtggtagag
     3241 gtagtatctg tactgtcact tgaagctgat gt
//
LOCUS       DAZAOG010000216         3253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000216 DAZAOG010000000
VERSION     DAZAOG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.219"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1328)
                     /locus_tag="ACHDLD_002710"
     CDS             complement(<1..1328)
                     /locus_tag="ACHDLD_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3364371.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVK"
     gene            complement(1392..1751)
                     /locus_tag="ACHDLD_002711"
                     /pseudo
     CDS             complement(1392..1751)
                     /locus_tag="ACHDLD_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1929..2249)
                     /locus_tag="ACHDLD_002712"
                     /pseudo
     CDS             complement(1929..2249)
                     /locus_tag="ACHDLD_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2365..2886)
                     /locus_tag="ACHDLD_002713"
     CDS             complement(2365..2886)
                     /locus_tag="ACHDLD_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364372.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
BASE COUNT          999 a          605 c          451 g         1198 t
ORIGIN      
        1 tccttaacat actgtctaaa tcgaatctct tgataggtgg gatatgttaa agctgaatta
       61 ttttggatat caacattctt ttgtctaaaa tatctatcat atttctcaaa aagctgctgc
      121 ttatcagtgt ctttcttcca cgtttgataa agatactcta taagttcgtt gatattaaaa
      181 acttcgtcta ctttttccaa atcatccatt tgaatatatt ttaattcatt ctgtcttttc
      241 ataaccttca tgccgttgaa attcacccat tgtggtaaat gatacatctg aatataaagt
      301 tcttccaaat catctaagaa ataaaaggca ttggcacagg tcaattgaat gatatttttc
      361 aaattgccaa cattttcact aaagcgatag tttaataaaa aatctaataa ttgttgagat
      421 atcagtattt tctttgctag tctttcttct tctgatttaa aaaatatctt gattaactgt
      481 tttctttcat aaagagaacg actgtctaat gttggaagtg ttagctttac aggtattctt
      541 ctcaacaaag ttgataatag tttatcctca ggtggttctg atgttgcaaa tataatccag
      601 caacttgatg tgtaccagtt ttcattatca cccatccgat gaaatttcct cgtatccata
      661 aactgaaaaa gtttctcctg acattcattg ctcaatgcat gaatttcatc taaaaataag
      721 actccattat tagctaattc tgctaaaccc tttcttccat cttcggctcc tgtatatgca
      781 ccttttacat gcccaaaaaa attggacaag aaaagttctg gattattggc atattcacta
      841 caattaactg aaatgaaaga tgccttctct tctatataat tattggccaa caaatcctta
      901 tacacataat ttgctaaata gctttttcca gttccagtct cgccatgaag taaaatgggt
      961 aagccatttg gcggatagta gattgctgct ttaatttgag caattacatt tcgcagactt
     1021 ccttgacttc cgataaccct ttctattcca accctaaaag agctatcata ccatttttcc
     1081 gcttcctctg aagagctaaa aatttgttga tttggaactt tctccggtaa agaaaagaag
     1141 attactggac gagtattgat ttttccaaat tctccatctt ttacgaattc attcaaatat
     1201 tgagagacaa tattacgact aacatttaat ttttcagata ttttctgtgc tgtattgttc
     1261 ccccatttat ttttaatctc agagttctct atgacataat tgacaatgcg ttcttttaat
     1321 tcttccatca cagcacctcc tcgacactat tcgaattttg ccgacagtat ttttccataa
     1381 atatgacttg ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc ttgttttgct
     1441 tgatccgcta agacgttgaa aagatactct ggcgaaatcg cataccccaa aatatcaacg
     1501 attgttgcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg atacaacatc
     1561 gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt
     1621 tccaacaaac ggtcggataa atatttatag aaaatcagcc ccaataaata atttttatat
     1681 tctgacgcat ccattttact acggagatta tctgccgcac taaataactt ctgatttaat
     1741 tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt
     1801 ttagcatact cagttttaga aaaagcaaaa aaataaagaa acttattctt gataacaagc
     1861 tacgtgacgt atatccctaa cacaaaaata gccatatcct ttggtttgga tacggctatt
     1921 tgatattact cattcaatta tcaaatcaca gtaggtgtga atctcatcgg gatcggcaat
     1981 atctacttct gattaggatc aaaggtgatt ttctcaataa aggtgtgata caaagcttta
     2041 acttgcttct tctcactctg gataagaaag caatccaaac tagtcaatat ctgttggaca
     2101 tctttgactt gaatcggttc cccttgttgt tgaaagatcc ctaagatttc attctctcga
     2161 atttgtaact gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa catcggaaac
     2221 aattcgggac tatcttccaa agcagcttcc tgtttttgct ctaatagtta gattttgcgt
     2281 accattttct tcttttattt tccttcgatt gctgatttta atatagtcta aaggaaattt
     2341 atatttattg tattctttat aacttcaaga gaacttcgtt tgagtattac tgtatttttt
     2401 taattccata agaaaattag ttattacctg ccacctaatc agcaaatctg aacctgagtc
     2461 aatataatta atattatttt ccatctccaa cattgtaagt actgttggtg ttaatatagg
     2521 gactttgtac aattgtattt ttttgatttt atcatctgca caaaggagat tttttaaatc
     2581 taatccttct tttatttcaa aagttgaatt ttttaaatct ataagagtaa aaccaattcc
     2641 ttcatactga atttctgaaa atcgaatttt tctcccagtt gatagatcaa ggggcagaaa
     2701 accttttatt acattgtgag tgtcaccatc atttacaatg tcattaggaa ttttgatttt
     2761 agaattctgt ggagcaccta ttggaagata ataattcatt ataattttgt agtcttcatg
     2821 aggaaataaa gtacttttag gattgctaag atattctcgt accgaatcta agaatggctt
     2881 atccattgaa tcttcatccc catttaaact aaaaaacatt cctacaattg cttttgcaac
     2941 tctaagtgaa ttcattttct ttattgaatg tgttaaatca gacttaccat tctttaaact
     3001 tgtattgtta taaaggaaat ttttattgtc ttctgcaaat ctaatatatt cctcaacata
     3061 ctcactacca aaaaaactat tacatttact acataacgta ttggaatggc aacagttttt
     3121 ttgacaaatt ttataaggtg cagaacttct ttccgtatgc tattccgatt gtccttgaca
     3181 atgagcctcc tcggatgtga cgatcttcgg caggagctaa cagttaaagt tagactgcct
     3241 cgctagcgtt agc
//
LOCUS       DAZAOG010000217         3244 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000217 DAZAOG010000000
VERSION     DAZAOG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.220"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACHDLD_002714"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACHDLD_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACHDLD_002715"
     CDS             complement(612..1160)
                     /locus_tag="ACHDLD_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364373.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACHDLD_002716"
     CDS             complement(1161..2018)
                     /locus_tag="ACHDLD_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN3364374.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACHDLD_002717"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACHDLD_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACHDLD_002718"
     CDS             complement(2580..2909)
                     /locus_tag="ACHDLD_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3364375.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..3066)
                     /locus_tag="ACHDLD_002719"
                     /pseudo
     CDS             complement(2986..3066)
                     /locus_tag="ACHDLD_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3131..>3244
                     /locus_tag="ACHDLD_002720"
     CDS             3131..>3244
                     /locus_tag="ACHDLD_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN3364376.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          962 a          646 c          421 g         1215 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacggt
     3061 tctgttgcaa agttttccaa aaaatctatt ttagtgtaaa attgagaaaa aagacagaga
     3121 ggacagagta atgaatcatt ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc
     3181 tgttggttac tacctgcgtt acaatctaag ctatcgtgaa gttcaggaat tgttatatga
     3241 tcgt
//
LOCUS       DAZAOG010000218         3199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000218 DAZAOG010000000
VERSION     DAZAOG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.222"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHDLD_002721"
     CDS             301..585
                     /locus_tag="ACHDLD_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364377.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHDLD_002722"
     CDS             588..1004
                     /locus_tag="ACHDLD_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364378.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHDLD_002723"
     CDS             complement(1141..1860)
                     /locus_tag="ACHDLD_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3364379.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHDLD_002724"
     CDS             2522..2608
                     /locus_tag="ACHDLD_002724"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3364380.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACHDLD_002725"
     CDS             complement(2910..>3199)
                     /locus_tag="ACHDLD_002725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364381.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAZAOG010000219         3089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000219 DAZAOG010000000
VERSION     DAZAOG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.223"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1813
                     /gene="alsS"
                     /locus_tag="ACHDLD_002726"
     CDS             149..1813
                     /gene="alsS"
                     /locus_tag="ACHDLD_002726"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN3364382.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1826..2536
                     /gene="budA"
                     /locus_tag="ACHDLD_002727"
     CDS             1826..2536
                     /gene="budA"
                     /locus_tag="ACHDLD_002727"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN3364383.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2666..>3089
                     /locus_tag="ACHDLD_002728"
     CDS             2666..>3089
                     /locus_tag="ACHDLD_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN3364384.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQ"
BASE COUNT         1032 a          515 c          694 g          848 t
ORIGIN      
        1 ttctttttca ttttttgaaa catatggcta aacaaatgaa ataaaacgtg ctatactttt
       61 gtatgaaaca ggtttagaaa ctgtatcact tatttgtgat tatttctaac taataattaa
      121 aaataaagca taaaaaggac gatgttcaat ggctgaaaaa atgaaacaag gttcgaaatc
      181 tgtagtagaa agtttgatta atcatcatgt tgattatgta ttcggtatac caggtgcaaa
      241 aatcgatggt gtatttaatg aattagagga tcaaggtcct gaattaatcg tgactcgtca
      301 cgaacaaaat gctgcattta tggcacaagc aatcggacgt attacaggag agccaggagt
      361 agttatcgca acaagtggtc ctggtgctag taacttagct acaggtttgg taacagcgac
      421 agcagaaggt gacccagttt tagcaattgc cggacaagta aaacgaagcg atctattgaa
      481 gttgacccat caaagcatgg ataatgcagc actgttccag ccaatcacaa aatacagtgc
      541 agaaatccaa gatccagaaa caatttcaga agtgattgcc aatgcttatc gtatggctaa
      601 gagttcaaaa aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg atgcacctgt
      661 ccaaggtaat gtcatcaagc cattgagcga tcctaaatta ggttctgctt ctgcagatga
      721 tatccgttac ctagctgaac gcattagaga agcaaaacta ccggtactat tagtaggtat
      781 gcgaggatca agtgaaaaag aaacgttagc catcaggcaa ttagttgaaa agacagcatt
      841 gcctgttgta gagacattcc aagcagctgg agtgatctca cgtgaattag aagcgcattt
      901 ctttggtcgt gtgggtttat ttagaaacca accaggagat atgcttttga aacgaagtga
      961 tctagttatt gcaattggct atgatccaat cgaatatgaa gcaagaaact ggaatgcgga
     1021 aaaagatgcg cggatcattg tgatcgatga agcgccagct gaaatcgatc cttttatgca
     1081 gcctgaaaga gaattgatcg gtgatatctc agccactctt gatttattga caggttcatt
     1141 agagcctcaa caagtgtcag aagatgcaaa agaatattta gcatctcttc aagcaaaatt
     1201 aacggagcgg gacattgttc aatcaaaagg agaagcaggt atcttgcatc ctttagaagt
     1261 tattaacacg cttcagtcaa aagtaacaga tgatatgact gttaccgtag atgtaggtag
     1321 tcattatatt tggatggcac gccactttag aagttatgaa cctcgtcatt tgttattcag
     1381 taatggtatg caaacgcttg gtgttgcatt gccatgggcg atttctgctg cattggtgcg
     1441 tccaaataca caaatcgtat cagtttccgg cgatggggga tttttattct ctgcccagga
     1501 tttagaaacg gcggtacgta aaaaattgaa tatcgttcat ttgatttgga atgatggaca
     1561 ctacaatatg gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt
     1621 tggtccagtt gattttgtga aatatgcaga agcttttggc gctaaaggaa tccgagctac
     1681 aagtgtagag gaattagaaa aagctttaga agaaggattt gcgacagaag gacccgtaat
     1741 tattgatatt ccgattgatt accgtgataa tgagaaattg ggagaaacaa tcttaccaga
     1801 tcaattctat taaggaagtg tcgacatgac tgaaaaaatc ttataccagc acgggacatt
     1861 aggagctttg atggccggct tgatggatgg aacagaaacg attgctcata tattagaaaa
     1921 aggaactctg ggattgggaa cattgcatgg gcttgatggt gaagtcatct ttttagatgg
     1981 cgtagcttat caaggaagat cagatggctc agttgcccaa ttagatggaa gtgaacttac
     2041 cccctatgca gcaattacag attttacgcc agatactagt ttttctgtct caaaaactgc
     2101 agatggagaa caattgaaaa aagacatatt agtaagagaa gccggggaaa acctattttt
     2161 ggcagtgaag ataactggat tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga
     2221 aaaaccttac agaagactag caaaaatttc agagtctcaa ccagaattcc aacaaagcaa
     2281 tatccaagga acattagtcg ggttctttac gcctgaatta ttccaaggag ttgctgcagc
     2341 gggatttcac ctgcatttta tcgatgatac acatacattt ggcggacatg taatggattt
     2401 tgaagtggct gaaggaaaag ttgagattag ccgcatctcg tcccttgtac agcattttcc
     2461 agttgatgat ccgacttttg ttcacgaaac gattgattat gctgatttag cagcagaaat
     2521 ccaacaagca gaatagaaaa agacagcgag atgcgttcaa ggtgattaac ctgacgtatc
     2581 tcgttttttt gaggaggaaa cctagcgttt acaaaaaaac tgagctataa tcttctccat
     2641 agatcaaaaa ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga
     2701 aattttatta ttacagcaac aaggattatt agaagcagaa gataactggt tagcaaattt
     2761 agcaaattca tctgcattgt tgaatgagac tttgcctgag actgtctttg caggatatta
     2821 tttattcgat ggtaatgaat tgattttagg accgttccaa gggcgagttt cttgcacacg
     2881 tatcaagatg ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa cattgatcgt
     2941 tgatgatgtc aaaacccatg caaattacat ttcctgcgat tcagccgcac aatcagaaat
     3001 cgtagtgcca atggtaaaag aaggtaattt aatcggtgtt ttagatatcg attgcggagt
     3061 aacgaaaggg tatgatgaaa tcgatcaag
//
LOCUS       DAZAOG010000220         3061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000220 DAZAOG010000000
VERSION     DAZAOG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.224"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..786
                     /gene="fabI"
                     /locus_tag="ACHDLD_002729"
     CDS             31..786
                     /gene="fabI"
                     /locus_tag="ACHDLD_002729"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN3364385.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(896..1948)
                     /locus_tag="ACHDLD_002730"
     CDS             complement(896..1948)
                     /locus_tag="ACHDLD_002730"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN3364386.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2074..2994
                     /locus_tag="ACHDLD_002731"
     CDS             2074..2994
                     /locus_tag="ACHDLD_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN3364387.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          891 a          618 c          588 g          964 t
ORIGIN      
        1 aatttttatc agaaacaaag gagagtcatt atgtcatttc tagaaggaaa aaaaatcgtc
       61 attatgggcg tcgctaacaa aaaaagcatt gcttggggtt gtgctgaagc aatgttgaaa
      121 caaggggcag aaattatcta tacttaccaa aatgaacgaa tgaaaaaatc attagtcaaa
      181 ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt ctagcgacga aagcatcgaa
      241 aaagcaatga atgccatcgg tgaatatgct ggggagatcc acggccttgt ccatgcagtt
      301 gcttacgcca acaaagaaga attgtccgga aatgtgtctg atatttccag agacggctat
      361 caattagctc aagatatcag cagctactct ttaattgctg tatcaaaata cgcacaaccg
      421 tttcttgcac caaacagcgg gatcgttagc atgacttatc taggttcaga acgcgcaatc
      481 ccgaattata acatgatggg tattgcgaaa gcttctcttg aagctgctat ccgttatttg
      541 gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa tctcagctgg ggcaatcaaa
      601 acattagctg tcacaggtgt taaagactat caaaaactga tccagttatc tgaatctcgt
      661 acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta atgcttgtgc tttcttaatc
      721 agcccacttt ccagcggaat catcggtgat attatctacg ttgacaaagg tgtccatctt
      781 tcataaactt ttgttgcatt ctttaaagta aaacatataa aaatcaggaa aaaacttttt
      841 aaagtctctt cctgattttt tctttttacg tttttttcaa ctcattactt tgaaattatt
      901 ccacgacttc aatccagcct tctggtgctt ctatgtctcc aaattgaata cctgtcaatt
      961 catcatacaa ccgttttgtt actggtccta cttccgtttc actataaaat acatgaagat
     1021 tgttattgtg ataaatccca ccgattggag aaataacagc cgctgtacca catgcaccag
     1081 cttctgcaaa ttgatctaga tcatcaatga aaatatctcc ttcaattgct tgcagtccta
     1141 gtcgttcttc agctaactgc aacaaggaat atttcgtgat acttggtaag atagagggcg
     1201 attgcggagt gatgaatttt ccgtcttttg taattccaaa gaaatttgct gcacctactt
     1261 cttcgatttt cgtatgtgtg gctggatcaa gataaacaca atctgagtaa tccagttctt
     1321 ttgcttctgt tcctggaagt aaactcgctg cgtaatttcc gccgactttt gctgcaccag
     1381 tccccttagg agcagcacga tcgtaatcag atacaataaa attcgttggt tttagtccac
     1441 ctttgaaata cgctcctact ggcgtacaga agacgacaaa taaatattct ggagctggat
     1501 ggacaccaat gttttcaccg accccaatca gtaaagggcg caagtataat gtagcgcctg
     1561 ttccataggg cggcacaaaa tcttcatttg cttttaccac ttgcttgaca gcatcaacga
     1621 acatcttttc agggaccatt ggcatcagca atcgtctagc actttgattc atccgtcgac
     1681 tattttgttc aggtcgaaag agattgacct tgccgtcttt tcgacgataa gcttttagac
     1741 cttcaaaaca ttgctgacca taatgaagcg ctggagagcc ttcatgaatc gtcaggtaat
     1801 tatcttctgt caattctcct tcttcccatt gtccgtcttt ccatttggca ataaaacgcc
     1861 aaggagtttt gatataagaa aaacctagat tgttccaatc gatttgcata tacttaccac
     1921 cttcgttatt ttctccacat tttatcatta tccatattat ttgtcatttt attttcagct
     1981 tttttccttc attatagtta tttttgctat ttcctttttt gaaaacttct tggtacaata
     2041 cagaaagaat caaattttag aaaagaggat tttatgtttt cattattagc aactgccggt
     2101 acgacagact cttctggcga gacattgaca gatcaagctg tcagcaaagt aaatgcgttt
     2161 cagcggtttt gggacgggat tgattgggac gccattttgg ctgcctttat ccaaaaaagt
     2221 ttatatctga tttttttgat cgtccttttt tggattcttc accgtgtggg aaaatatctg
     2281 attgataagt cttttaatca gtattctaaa aaagccattc taaacgagag ccgcttaaag
     2341 actttacata gtcttttaaa tactattttt cattataccc ttggtttctt ttttatctat
     2401 gcaattttat ctgctatcgg tgtgccgatc ggatcacttt tggctggtgc cggaattgca
     2461 ggtgtggcaa tcggtcttgg tgcccaaggg ttcatgagtg atgttattac cggatttttt
     2521 attatcatgg aacaacagat ggatgttggg gactacatca agctagcaaa cttaacgatt
     2581 gaaggaaccg tcgtatctgt tggtatccgt acccttcagc tgaaagcaac ggatggtact
     2641 gtccatttca tcccgaatcg aaacatcacc acgatcagca atctctctcg tgcaaatatg
     2701 caagtacttt tagatattcg gattgttcct gaagaaggat atgacaaaat ctatgagatc
     2761 attgatcgag taaatcgtac attagctgaa aaataccaag aagagctcca aactgaacct
     2821 tctatctttg gattagtgga tcttggaaac agcaattttg cgattcgtac ggtctgctac
     2881 gcattgaacg gaaaacaata tgccttaaaa gaagagttct tgagctccta tgtcaaagaa
     2941 ttgacagctt ctggtttcac gattccgaac agtccaattg ctgtcggaaa ataatttgga
     3001 caaacgatgc aacgaatctc actaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     3061 c
//
LOCUS       DAZAOG010000221         3046 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000221 DAZAOG010000000
VERSION     DAZAOG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.225"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..898
                     /locus_tag="ACHDLD_002732"
     CDS             <1..898
                     /locus_tag="ACHDLD_002732"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3364388.1"
                     /translation="QLWIGGIFLIDVILLAKGTISITNFVIVFMYANMVPTLWTTLIS
                     IIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNISFS
                     TEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISIAT
                     QIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKILLS
                     GGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYIIITHDHI
                     LDNFLDSNDEILYLNKEYDNNE"
     gene            891..1919
                     /locus_tag="ACHDLD_002733"
     CDS             891..1919
                     /locus_tag="ACHDLD_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFN3364389.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1975..2193
                     /locus_tag="ACHDLD_002734"
     CDS             1975..2193
                     /locus_tag="ACHDLD_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364390.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2264..>3046
                     /locus_tag="ACHDLD_002735"
     CDS             2264..>3046
                     /locus_tag="ACHDLD_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364391.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYRE"
BASE COUNT         1198 a          340 c          520 g          988 t
ORIGIN      
        1 ccaattatgg attggaggaa tatttcttat tgatgttatt ttattagcta aaggaacaat
       61 aagtattact aattttgtaa tagtatttat gtacgctaac atggtcccta ctttatggac
      121 tactttaatt agtattattt ggaaatggca agactattca gtgtcattaa atagatatta
      181 cgaatattat tcaaataaca acaaagtggc tggcacaaaa aaaataaata aaataaattc
      241 tattactggt caatcattaa atttttcata taacaaaaga aaggtattaa gcaatataag
      301 tttttcaact gaaatgaata aactattcat gatatcagga gaaaatggaa cagggaaaac
      361 aacactgatg aaattattta ctaaacagtt gccggttagt aataatcaat tattcgtcaa
      421 cgggatagat attaatatga ttgataacga atcattaaga aggttaattt caatcgcaac
      481 acaaatacca tatataatta atagctccgt gaaggagaat attttaatgg ggactaaaaa
      541 ctatgataaa aaaaaattca gtaaattaat agaactattg ggaataagta aatttatgac
      601 gaaattaggt attactttgg acgacaatat ttatgatgaa aaaatattat tatctggagg
      661 agaaaaacaa aaaataagtt tagctagggc gcttattaaa tcagcttcag ttattatatt
      721 agatgaacca tttagaaatt tagataatga aactcgcgat aatttgaggg attatatact
      781 aaatacaaaa aaagaagttt gttatataat tataactcat gatcacattc tagataattt
      841 tttggattca aatgatgaaa tattatattt aaataaggag tatgataata atgaatagca
      901 aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt gatgttacat
      961 ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac aattgtttta
     1021 tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata tgtaatttaa
     1081 agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa atagttttat
     1141 cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt actaatggat
     1201 cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat cgatttcaag
     1261 taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat ccaaacagtt
     1321 ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa gtggatgttg
     1381 ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa ctagtaaata
     1441 ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg ggaatgcaaa
     1501 atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat attaactata
     1561 ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat catatcatcc
     1621 gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca gacggaggat
     1681 taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac agtctttcta
     1741 aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa gatatagcta
     1801 ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat attaaatggc
     1861 ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt ttaatttagg
     1921 tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa aaaaatgatt
     1981 tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc tggtgaagct
     2041 gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc aggagtaggt
     2101 atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt agctgcagcc
     2161 gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta actgtctcct
     2221 attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat acaataattt
     2281 aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt atggatattt
     2341 aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc caacagtaag
     2401 atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa
     2461 aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag gaactattaa
     2521 atttttcaaa cactataata ttattgatat aggagattat agtatggaag aaaagaaaat
     2581 atataaaatt gaacaaaatt tagaattaga attactaaat gaatcacatt atgaagaaat
     2641 ttataatttt atcacagatc atcctgaaag tatgtatttt catgaagata tttatcatca
     2701 aggaaatagt aaagaattag aagttgtcct tagatataat ttattttcgt ttacgagtga
     2761 attttacttt ttaagacata aagagagaat tgtaggaatg gtttctataa ttgttaatcc
     2821 atttcaaaac ttatcctaca gtatagtaaa ttacctcata tgtgagcctg catatactac
     2881 gactattttg acaacagtat tagaaaatat taacgaagtt tcaattttag aagtaaagaa
     2941 gctagtgatt catttgctaa attcaacaga agatgaatca ctaatcccct atttaaaaga
     3001 ttcacatttt tttaaagaag cggtactatc taaagaatat agagag
//
LOCUS       DAZAOG010000222         3024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000222 DAZAOG010000000
VERSION     DAZAOG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.226"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1243
                     /locus_tag="ACHDLD_002736"
     CDS             <1..1243
                     /locus_tag="ACHDLD_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3364392.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="ACHDLD_002737"
     CDS             1256..1888
                     /locus_tag="ACHDLD_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3364393.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2096..2320
                     /locus_tag="ACHDLD_002738"
     CDS             2096..2320
                     /locus_tag="ACHDLD_002738"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFN3364394.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2422..2655
                     /gene="relB"
                     /locus_tag="ACHDLD_002739"
     CDS             2422..2655
                     /gene="relB"
                     /locus_tag="ACHDLD_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN3364395.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="ACHDLD_002740"
     CDS             2645..2914
                     /locus_tag="ACHDLD_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3364396.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DAZAOG010000223         2952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000223 DAZAOG010000000
VERSION     DAZAOG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.227"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..423
                     /locus_tag="ACHDLD_002741"
     CDS             <1..423
                     /locus_tag="ACHDLD_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364397.1"
                     /translation="YSFVRPYVYTKNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRII
                     GECSNPYWEVIQNSKVVASDGYFINMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQ
                     QDFTKSNFVQAPLGEFSVVFHVGHANVEIELYEERDLF"
     gene            420..1469
                     /locus_tag="ACHDLD_002742"
     CDS             420..1469
                     /locus_tag="ACHDLD_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364398.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            1473..2297
                     /locus_tag="ACHDLD_002743"
     CDS             1473..2297
                     /locus_tag="ACHDLD_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364399.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            2315..>2952
                     /locus_tag="ACHDLD_002744"
     CDS             2315..>2952
                     /locus_tag="ACHDLD_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN3364400.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         1079 a          418 c          580 g          875 t
ORIGIN      
        1 tactcatttg ttcgaccata tgtctacacc aaaaatgtat gggaaaagaa aggtgtattt
       61 aagttcagta ataattctgt atatttgaca aatagtacag agcgaatgtc tccgttaaaa
      121 ataaggatta ttggagagtg ctcaaatcca tattgggaag ttatccaaaa ctctaaagta
      181 gtagcttcag atggctattt tattaatatg aaagacacac aaatattaga agtgtctagt
      241 ttgctagaag accaaacggc aattttaaaa gatgtaggtg gagttgaatc ttctgtttat
      301 caacaacaag attttactaa atcaaacttc gtccaagcgc ctctaggaga attcagcgtt
      361 gtttttcatg ttggacatgc aaatgtagag attgaacttt atgaggagcg tgatctattt
      421 tgattttagc cattaccctc tttcaccgag atatgaattt atataacgaa cgtttaattt
      481 cgactgagtt cgaatttgga acggatgaaa ttggagaaga agtaagtaat tttattacag
      541 ataaatatgt ccatgtaaaa tcaggagatt ttctactagc aaaatttatt cctagcggga
      601 agattgctta ttttggagtg attagctcgc aagaagacaa aaaaataaat tgcaaaggat
      661 tactcagttt ggccgatagc gagataccga ctgtaaaaat atcaggtgct aattatgaag
      721 agcatattcg acgactgatt gaatactact tattgaacga tccaacaaaa caactaaaaa
      781 atattttgga ggtaaaaaca gaaagcgtta cttctcattc ttatcaagct gttgacacaa
      841 ataagagacg attgaatgca tatataataa atgggttcaa aaaatataat gttaaatggt
      901 atgtcaaaga gattaaaggt aaaaaaatat atactggtat tcgagcagtt gatgattcaa
      961 tttatattaa agataattca tctgagttta atgactggga tgtttttgtg caatctcctg
     1021 gaccagggaa cgagaacaaa ttattgatcg tagataaagc aatgaaagat atagaaaatc
     1081 ccttggtatt gtctacgtgg tatttagatg acgaaaataa tcttacgcaa gattcaacga
     1141 ataagaacat tgcattgcct accgttaatt tagtaaatat atatgatcaa actcaagaag
     1201 atagaccttc ttatgaagag gtagctaaat cggagcttaa aggcaacgcc tattcacatg
     1261 aaattaaagt taatgttgtg ctaaatgcta gaaatatcaa tgtagaaaat atcgaaacag
     1321 gaatgttagc gactattgtt tacaaagagc agacttacaa atctgttcta actgcctggc
     1381 gaatatccag cagtaaaaag aatatagaat tgacatttgg taatgtccga agtcgtttta
     1441 tggattattt tgaagataat gggggataaa aaatggttag caatgtggat ggatatcaat
     1501 ttgaaaacgt gaaagtaagc gcagaaaatg atgctagact ttatcacgtt ttatataatc
     1561 ggaaaaatca ggttattgat ggttacgatc agtctatgaa tttatcttca agcggattaa
     1621 cagtaaaagt tgctgcagga gcagcgatta ttcaaggtcg tatggtcgtt gttcgacaag
     1681 aagaaagtat aacagttcca gcaaactcaa gtggttatat agcattaaca gtggatttga
     1741 cacaagaagt tatacctgga tctattcttc cagaatcgga agaatatgaa tggactaata
     1801 atcaagtcaa gctagaattt ataacaaaag ttataaaagg taatttaaac aatggtgata
     1861 aggtctataa tttaccacta tgctcagtta attctactgg atcaactgtt tcaatctcaa
     1921 agatatcgga tagttacgag ctgactctct ctaaaggaga aattttgtgg agggggactg
     1981 cgttaatgca tgatactcaa actgtccaac cttcaaaaaa aatttggcag acagtttcag
     2041 gttttttgtt agtatggctc ccatacgaaa acgggcaagc aattgaggat agatatgtaa
     2101 ctacgccttt ttataaggag cgtataactt ttactaatgt tttaggagaa attgtttcag
     2161 gatttgatga ctatcacaaa aagtggttta gtaaacgaat aaactataat tcgaatacta
     2221 atatatttac aggtgcagca agcaatgcaa gtggagataa cgcaaatatg gtgcttaggt
     2281 atatagtttc tttttagtta ggaggtatgg aagagtggca aatttagaaa ttaaattatc
     2341 tgcaaataag agacagcctt atctacgtca ccgtgttgtt ggtagagttg gtgatggggg
     2401 gctcacaaca atcaatgtac aacttcttga ggaagatgaa attacacctt ttgtaattaa
     2461 tctaaacggt actttgaaat ttgtgggcga agtttcaaac ggtaactata ccgagggaga
     2521 accagaaata atcgattcga ctaatgggtt aattagttac acgttcacta agtcaaattt
     2581 cagcacgagt catcaattca aacaagcata ttttgaatat gtagatccta acggcaaaaa
     2641 agtaactttt cagaacttca tcatagacgt gttagaacga gtagatatta attcggagca
     2701 agcgaaatac tatatttctt cattggaaaa attacagagt gaaatgcaaa ccactttcaa
     2761 tcagttcatt agtgataaac aggtccaata cgatcaaatc tactcgaaat ataacgaatt
     2821 agtaagattg ataaatgaat cagataagca agtaaatgat cgtattgata aggcaaataa
     2881 tcgtattgat caaaccaatc agcaaatcgg cgatctcggc aagctgaaaa agatgtacag
     2941 taacagcatt ga
//
LOCUS       DAZAOG010000224         2876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000224 DAZAOG010000000
VERSION     DAZAOG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.228"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACHDLD_002745"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACHDLD_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACHDLD_002746"
     CDS             1122..1634
                     /locus_tag="ACHDLD_002746"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364401.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACHDLD_002747"
     CDS             1631..2668
                     /locus_tag="ACHDLD_002747"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3364402.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1040 a          508 c          573 g          755 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgca
//
LOCUS       DAZAOG010000225         2875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000225 DAZAOG010000000
VERSION     DAZAOG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.229"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..443)
                     /locus_tag="ACHDLD_002748"
     CDS             complement(<1..443)
                     /locus_tag="ACHDLD_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364403.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="ACHDLD_002749"
     CDS             513..863
                     /locus_tag="ACHDLD_002749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3364404.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="ACHDLD_002750"
     CDS             1277..2095
                     /locus_tag="ACHDLD_002750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3364405.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="ACHDLD_002751"
     CDS             complement(2168..2527)
                     /locus_tag="ACHDLD_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3364406.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          914 a          439 c          530 g          992 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttcca
//
LOCUS       DAZAOG010000226         2862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000226 DAZAOG010000000
VERSION     DAZAOG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.230"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..113
                     /locus_tag="ACHDLD_002752"
     CDS             <1..113
                     /locus_tag="ACHDLD_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3364407.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(199..1293)
                     /locus_tag="ACHDLD_002753"
     CDS             complement(199..1293)
                     /locus_tag="ACHDLD_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN3364408.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1482..2243
                     /locus_tag="ACHDLD_002754"
     CDS             1482..2243
                     /locus_tag="ACHDLD_002754"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3364409.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2358..2798)
                     /gene="fabZ"
                     /locus_tag="ACHDLD_002755"
     CDS             complement(2358..2798)
                     /gene="fabZ"
                     /locus_tag="ACHDLD_002755"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN3364410.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          915 a          571 c          480 g          896 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 taacctataa aaaaacaaaa taaaaaagca gaaataactg gctatttcta gcggattatt
      181 tctgcaataa ctaattatct attttttatt ttcaattaat ttaaagcttg accatggttt
      241 caaaaagtca aggaggaata attcgtcatc ggagattctt cctacaacat tgacacgtcc
      301 gtcgtttttc atatccttca aggcgatttg tgtttctcct ttgtattgac cgtacccttc
      361 attgtcaatc agtacatccc catgatgcat atctcgcgta ttatgaggcg ggaaatcttt
      421 atctttgtag tatacacgag tcatcgttga acgaaggata tattctgagc gatcccctcg
      481 ataactatga aggttatcaa acaagcagat tcgttcgttt tctgtaatac cttcttccgt
      541 atctactttc aatgttggat aatccgcgtt aaatgcttct gccatttctt tcaattcttc
      601 ttcacttgcg tacgcatttc caatagaaat atcatcaatc aatcctgtaa ggattaaatg
      661 cttcacttgt gtcgcaattt caagatctcg gtggtcttcc agcgtgcaca agccatcttg
      721 cgtaggccaa gggccaaact cagcagattg cgaattgacg aatgccatcg tattcaaatt
      781 atattttctg aatttttctg aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg
      841 atgaggataa aaattatgag aacctaataa attatccgtg tttggcgaat aactcatgat
      901 gttgtcaacg taattcgtgc ccgaactcat attgatctcg attttgatac caaaaggatt
      961 gcgcgtcatt tttgcttctt ctgctcctgt gaatccgata tctaagcgga ccccgtaggc
     1021 acccatctta tggaaaaaag ataagtcatc gtaagaaatc tccaactgtt caaataacgc
     1081 tggattgata tcaaccatta cttccatgcc taggctattc gcgtaatcaa ctacttcttt
     1141 aaattctgaa agaacttttt ctttatcatc attgatctgc agcaaactgg taaaaacccg
     1201 tttataccca tacttatgag ctaaatccaa gtacgctttg tccttttcaa atgttgaccg
     1261 ttctggataa atcgagattc ctaattttcc catattacca tttcctcctg catcttttcg
     1321 tttttgtatc cacttacatt atagcaaaca caaagtaaat ggtctacacc ttttcttttt
     1381 cttttaaaat aaatgataaa ttccaataat ttaagtaggt attttccctc tattttgtta
     1441 tcattaaaga taacaaaata gtaaattgga aaggaacagt tatgaagaaa aataaagtta
     1501 ttttaagtat tagtttagca gcagcattgc tgtttgctgc tggttgttct gcaaacaaca
     1561 cgacttcttc tagttcgtct acagaaagtt caaaaagtac atctactgcg gaaagcacga
     1621 ctgccagtag cgtcgatttg aacgcattgg aattaccaca attgacgaaa gatgtcgccg
     1681 aaaatgaaga tctggtcgaa atggtgacga ataaaggcac aatcaaaatc aaattatttc
     1741 ccgaacacgc accaaaagcg gttgaaaact ttatgacaca tgcaaaagac ggttactaca
     1801 acggactgac tttccatcgc gtcatcaaag atttcatgat ccaaggtggc gatcctaaag
     1861 gcgatggcac tggaggggaa agcatttggg gagaaggatt tgaaacagag atcaacaacc
     1921 acctttataa tataagaggt gcgttatcta tggctcgctc acaagatcct aacagtaacg
     1981 gcagccagtt ctttatcgtt caaaatacag atgatatgca tgatggtctt ttaaaagacg
     2041 actatcctca agcaatcatc gacgcttata aaaacggcgg ttatccttca ctagatggcc
     2101 attatactgt cttcggccaa gtgattgaag gaatggatgt cgtggatgct attgcaaata
     2161 tcgaaacaga tagttcagac aaaccaaaag aagaagtaaa aatcgataaa atcaatatac
     2221 tgcaagaagc aaaacaaaaa taaaaaaata acgtctgaat cctgcataga tgacaacgta
     2281 tgctaggatt caggcgtctt ctttttacat aaaaagcaag taagcttcct tttgttttgc
     2341 tatatcaggt tttatagtca agcattttgg ttgacgatga acgtgaactc gcactcacag
     2401 acttttttat catctacata tgctgcagat aacgctgtac cgactgatcc cttcattttg
     2461 gtgatttcaa aatgacattt cagcacatct cctggaacaa ctttttgacg gaatttggcc
     2521 ttattgatcc caccgatata agctcttttt cctagaaact cttctgattt taggataagt
     2581 atcgaacctg cttgtgccag tgtttctaga atcaacgcac ccggcatcac tggattgtca
     2641 ggaaagtgac cttggaaaaa atcttcattg attgttacat tttttgtcgc aacgatcttt
     2701 tttcctggtt ctaaagtatc tacataatca atataacaaa taggataacg attaggaatc
     2761 aattccatta cttcagtagc agataatacg taagacataa ttaaactcct tctttcaggc
     2821 aattgccatt ttcattactt tgactatcaa agtatcatat tc
//
LOCUS       DAZAOG010000227         2845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000227 DAZAOG010000000
VERSION     DAZAOG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.231"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..307
                     /locus_tag="ACHDLD_002756"
     CDS             <1..307
                     /locus_tag="ACHDLD_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364411.1"
                     /translation="EVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNTDLK
                     RLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLRSL"
     gene            411..1589
                     /locus_tag="ACHDLD_002757"
     CDS             411..1589
                     /locus_tag="ACHDLD_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3364412.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(1770..1955)
                     /locus_tag="ACHDLD_002758"
     CDS             complement(1770..1955)
                     /locus_tag="ACHDLD_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364413.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            1992..2285
                     /locus_tag="ACHDLD_002759"
     CDS             1992..2285
                     /locus_tag="ACHDLD_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN3364414.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(2373..2519)
                     /locus_tag="ACHDLD_002760"
                     /pseudo
     CDS             complement(2373..2519)
                     /locus_tag="ACHDLD_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1020 a          419 c          542 g          864 t
ORIGIN      
        1 ggaagttaaa acaaatgtta attctctgga aatagagttc tattcaattt tacaaaaaaa
       61 actggaagat tacacggaaa aattgcttgc agtcagtcat ttacctctgg tattaacaaa
      121 tactgattta aagcgactat ttcagataag tgatagtaca ctgaaccgtt tagtaaaact
      181 tggtgagttt cctccctgtt ggtacgggat tcgaggacac tattttcgtg atgatgtttt
      241 agaatggatg agaaagagtg attcgaatga ctatcgagaa aaattgcgac tacttcgatc
      301 tttataagtt acattttctt tctaagatgc ggtacaatga tgacatagat agaaagattt
      361 ccaaagggtt tctgcttgtt gggttccttc aaaataggag gaatcaataa atggaaaaaa
      421 cagatcgatt caagaagcaa atcagttcta ctggtaggat cacatggtac tttcaagcat
      481 atcgaacaac aagacgtggc tttaattcaa aacgtgaggc gcaactcgct tatctggaaa
      541 tggagaaaca acaaaggctt aagaaaaaaa ttgttgcaag caatgacaag tttagtgttg
      601 taggtgagca atggttccaa tattaccgtt cgttaaatga acaaaaggaa ataacctatg
      661 ataagcgaga ggaacaatta aaaattattg ttcgatggat tggtgataaa aagttgtctg
      721 atttgacacc agagtgcctt caagaattgc tttttacctt aaaggaatgt ggcgttaatg
      781 gaacaaatgt tggctatgcc aaaaatagtt tacaatcact tattcaagtt ctaaatatga
      841 tttttaaata ttgtcaaaga aagaatttga taagtgaaaa tccaatgaag tctgtgagaa
      901 tgccaaaata tcagttaacg gtaactgatt taaaagaagc cataaataat attgaggaca
      961 agtttcttac tgtcgatgaa ttgagaactt ttttgaatta tggcattgtt catgaggaat
     1021 taccgatgtc tgtacttttc catgttctat tttacactgg ctgccgagtc agcgaggcgc
     1081 ttgctctaca gcctggagat atagatttca aaagaaatga aattctgttc tataagcaaa
     1141 cggcagttaa aggtaagagt aaggattttc gaattgaaac tactaagaca gttagttctg
     1201 ctagacgagt tctagtaact cctctagtta tggaaaagct acaacagttg attaaggctt
     1261 tgggtgagat gaggcaacat tacaggttta ttatagaaga aacatatttg tttgtgtatc
     1321 ttgattctag taaacgtggt gttccttatc gtcgagagta tgtaaatgat cacgtcaaac
     1381 ggtgcgtgaa tcgctcagga attaataagc catttcatac tcatttagct cgtcatacaa
     1441 tggcaagctt agtagcggaa cactgtagct gggaagtctt gaaagatcgc ttaggccata
     1501 ctgaccaaac aacttcgaaa atttatcgcc acttaacgtc aaatgaaaag gtaaaacctc
     1561 tgattgcttt tagttcttta gagagttaaa aaaacggtga aagcactggt attacaggat
     1621 tcttgaaaaa cgatttttta acaaattagt caatatgtag tcagaggtaa taaaaatcct
     1681 tttatatcaa tactttaacg acttttcaca agtatcaaag aaatccgtcg ttaatcattg
     1741 ttatcagact gagaaagaac ccttggtatt caagggttct tttttcttag catgtaattt
     1801 gaacaccctg atccagtttt ttctttaatt attggtaggg gagaataatg gattagaata
     1861 aatctaccta taagtagtag taaaaaaaag agcactatga taatatcttt cttaagagta
     1921 accgctttaa taaaaaatcc agaatattct aacataaatg gagtattctt agaatcaaaa
     1981 ggagaaagaa gatggtttat acttatacgg agcaagaaaa gatgaaagtt ttagacacat
     2041 tcatgaatga tggaaaagta aaacaagtac caagttcaac aaaaaagaaa tacatcctat
     2101 tgaaagaagt cttgaaacgc tttgaccatg gagtgactta tacagaaaca gaggttaatt
     2161 cgatactgct gaacgtattt tctagtggcg attatgtcga acaaagacgt tatctaatca
     2221 catttggctt tttcaaaaga agttcagacg gtcgggcata tcagatgatg ggaattgaaa
     2281 attagaagtt ttaatttata ctcattgaaa aaagttcaag tatgcgatat tggttctaca
     2341 ccgcatactt gaactttttt tgtcatctgt atctataata caaagccaag aacgttattc
     2401 aaaatatgga tagctatgat aaaagctaat gagcgattcg cagttaaata agcaatcgtc
     2461 agtaaaatcg ttccaggagc gtacatgaaa aaatcaattg gatgcgttac atgaataaag
     2521 ataattctct tttatttgta cgaataaata ccaaaacgta cctcctaaaa ttagattttg
     2581 gacagatgcc ctttaaaaag ataacaagat ttcgttcttg aaacaatata tatgctttta
     2641 ttccaatgag gttgtgaaaa cagtattttg tcctgagcgt tagaaaattg aaaatgggga
     2701 atagcaactc gtactttacc attttttaag tgaatacaag atcagctaat tgaacaccgt
     2761 ttattcgtga tgagggacac aacactgcag tcgacacatt tctcttttat gttcaaagat
     2821 aacaaatatt taaaacaaag aaaaa
//
LOCUS       DAZAOG010000228         2739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000228 DAZAOG010000000
VERSION     DAZAOG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.232"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHDLD_002761"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHDLD_002761"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN3364415.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAZAOG010000229         2685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000229 DAZAOG010000000
VERSION     DAZAOG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.233"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..2166)
                     /locus_tag="ACHDLD_002762"
     CDS             complement(553..2166)
                     /locus_tag="ACHDLD_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3364416.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..>2685)
                     /locus_tag="ACHDLD_002763"
     CDS             complement(2307..>2685)
                     /locus_tag="ACHDLD_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3364417.1"
                     /translation="LLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQL
                     AQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSI
                     HHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          853 a          495 c          465 g          872 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaa
//
LOCUS       DAZAOG010000230         2671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000230 DAZAOG010000000
VERSION     DAZAOG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.234"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACHDLD_002764"
     CDS             174..518
                     /locus_tag="ACHDLD_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN3364418.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACHDLD_002765"
     CDS             519..938
                     /locus_tag="ACHDLD_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN3364419.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACHDLD_002766"
     CDS             complement(1030..1380)
                     /locus_tag="ACHDLD_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364420.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2671)
                     /locus_tag="ACHDLD_002767"
                     /pseudo
     CDS             complement(1346..2671)
                     /locus_tag="ACHDLD_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT          994 a          480 c          523 g          674 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt t
//
LOCUS       DAZAOG010000231         2636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000231 DAZAOG010000000
VERSION     DAZAOG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.235"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHDLD_002768"
     CDS             190..690
                     /locus_tag="ACHDLD_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN3364421.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHDLD_002769"
     CDS             787..2634
                     /locus_tag="ACHDLD_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFN3364422.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
BASE COUNT          915 a          335 c          580 g          806 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaa
//
LOCUS       DAZAOG010000232         2567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000232 DAZAOG010000000
VERSION     DAZAOG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.236"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1045
                     /locus_tag="ACHDLD_002770"
     CDS             38..1045
                     /locus_tag="ACHDLD_002770"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN3364423.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1057..2061
                     /gene="neuB"
                     /locus_tag="ACHDLD_002771"
     CDS             1057..2061
                     /gene="neuB"
                     /locus_tag="ACHDLD_002771"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFN3364424.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2058..>2567
                     /locus_tag="ACHDLD_002772"
     CDS             2058..>2567
                     /locus_tag="ACHDLD_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN3364425.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFP"
BASE COUNT          941 a          329 c          513 g          784 t
ORIGIN      
        1 aattatagat gactataatt cataggagga gatataagtg tttaaaggaa aaacaatatt
       61 agtaactggc ggaactggtt ccattggaag tgaaattgtt cgtcagcttt taaaatatga
      121 accaaaagct atcagaattt atagtagagg tgaagaaaaa caattttata tgcaacaaga
      181 gctctcaaat cacactaatt tgagattttt aattggtgat atcagagact ctcaaaggat
      241 tgattatgca tgtcgtgatg tggatattat ttttcatgct gccgcaatga aacacgttcc
      301 agcaagtgaa tataatccta tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt
      361 aatagatgca gcgatcaaaa ataatgtaaa acattttgta ggtatcagta cagataaagt
      421 agtgagtcct actaacacca tgggtgcgac aaagttattg agtgaaaaat taataatagc
      481 tgccaatcag tataaaggaa aaacagaaac aatattttct tgtgtaaggt ttggaaatgt
      541 catggggtca agtggatctg ttattcctct ttttagtaaa caaataaaaa acaataaaga
      601 tattactttg acagatgttg atatgacacg atttatgatg tcaataccgg atgctgcaag
      661 attagttctg tctgctgcta tgaattcaaa atttggagaa acatatgtct taaaaatgcc
      721 ggcaatcaat attttaactt tagctcaatc attaataaaa atatataatg atacacatga
      781 tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa aattatatga
      841 agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa tgtatatcat
      901 tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac
      961 tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta gaatcgcaaa
     1021 agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt atatgtaatt
     1081 gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag tattgataaa
     1141 gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt
     1201 tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga cagttctcaa
     1261 tttgaaatgc taaaaaaatt agaattatct caatctgatt ttattagaat aaaagattat
     1321 tgtatacaaa ataaaattga ttttttgtct actccttttg atgaagaaag tgcagacttt
     1381 ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac aaatttacct
     1441 tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg tatggctgat
     1501 attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac tattttacat
     1561 tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat taacacatta
     1621 aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata tgatgttggt
     1681 atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt ggataaggaa
     1741 ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga ttttgtaaag
     1801 catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat gccttctgaa
     1861 cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt aaatgtggga
     1921 gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat agctcctaag
     1981 tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg
     2041 cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga gctgattatg
     2101 gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta tcatttgttg
     2161 tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc ttagctgacg
     2221 gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag ggaaacatgg
     2281 ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa actagccctg
     2341 attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata gcttgtttgt
     2401 atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact gtagatgaat
     2461 caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg gaaaaaagca
     2521 aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttccc
//
LOCUS       DAZAOG010000233         2483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000233 DAZAOG010000000
VERSION     DAZAOG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.238"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..1242)
                     /locus_tag="ACHDLD_002773"
     CDS             complement(163..1242)
                     /locus_tag="ACHDLD_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3364426.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1592..2440)
                     /locus_tag="ACHDLD_002774"
     CDS             complement(1592..2440)
                     /locus_tag="ACHDLD_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3364427.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          613 a          562 c          554 g          754 t
ORIGIN      
        1 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
       61 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
      121 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
      181 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
      241 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
      301 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
      361 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
      421 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
      481 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
      541 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
      601 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
      661 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
      721 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
      781 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
      841 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
      901 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
      961 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     1021 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     1081 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     1141 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     1201 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
     1261 attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca
     1321 aatggctaag ttaattataa aatccgccaa caaaaataat aaaaaaaaga aaagataaat
     1381 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     1441 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     1501 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     1561 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     1621 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     1681 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     1741 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     1801 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     1861 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     1921 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     1981 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     2041 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     2101 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     2161 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     2221 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     2281 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     2341 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     2401 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     2461 ataagattat cattttttcc gtt
//
LOCUS       DAZAOG010000234         2407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000234 DAZAOG010000000
VERSION     DAZAOG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.240"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHDLD_002775"
     CDS             5..1150
                     /locus_tag="ACHDLD_002775"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN3364428.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHDLD_002776"
     CDS             1321..1779
                     /locus_tag="ACHDLD_002776"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN3364429.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACHDLD_002777"
     CDS             1938..>2407
                     /locus_tag="ACHDLD_002777"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN3364430.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZAOG010000235         2391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000235 DAZAOG010000000
VERSION     DAZAOG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.241"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..253)
                     /locus_tag="ACHDLD_002778"
     CDS             complement(8..253)
                     /locus_tag="ACHDLD_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364431.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(270..1121)
                     /locus_tag="ACHDLD_002779"
     CDS             complement(270..1121)
                     /locus_tag="ACHDLD_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3364432.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1287..2291
                     /locus_tag="ACHDLD_002780"
     CDS             1287..2291
                     /locus_tag="ACHDLD_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN3364433.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          763 a          404 c          482 g          742 t
ORIGIN      
        1 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
       61 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
      121 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
      181 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
      241 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
      301 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
      361 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
      421 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
      481 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
      541 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
      601 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
      661 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
      721 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
      781 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
      841 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
      901 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
      961 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     1021 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     1081 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     1141 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     1201 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     1261 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     1321 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     1381 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     1441 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     1501 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     1561 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     1621 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     1681 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     1741 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     1801 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     1861 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     1921 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     1981 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     2041 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     2101 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     2161 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     2221 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     2281 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     2341 ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt g
//
LOCUS       DAZAOG010000236         2390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000236 DAZAOG010000000
VERSION     DAZAOG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.242"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACHDLD_002781"
     CDS             197..2059
                     /locus_tag="ACHDLD_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3364434.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACHDLD_002782"
     CDS             2059..>2390
                     /locus_tag="ACHDLD_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3364435.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZAOG010000237         2371 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000237 DAZAOG010000000
VERSION     DAZAOG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.243"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..791
                     /locus_tag="ACHDLD_002783"
     CDS             75..791
                     /locus_tag="ACHDLD_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3364436.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            810..>2371
                     /locus_tag="ACHDLD_002784"
     CDS             810..>2371
                     /locus_tag="ACHDLD_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFN3364437.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPR"
BASE COUNT          932 a          273 c          451 g          715 t
ORIGIN      
        1 attatttcaa tgattaagag tcgagagcac aaacggtaga cgatgttatt tttataaaaa
       61 gatataattt attaatggtc gataattatg aaaggaaaaa tattcttaaa aaaaagagta
      121 taggatgtcg tatgaaaata gtcgctatag taccaatgaa gttaaataac aaaagattgc
      181 cacaaaaaaa cacgaaagct tttaccaatg gaaaaccatt gtgttattat atactttcaa
      241 cacttttaaa agtaaaacag attgataaag tgtatgtcta ttgtagtaac ccagatatta
      301 aagaatttat accagaaggt gtaacatttt taaagagatc tagttcctta gatcaggata
      361 caacgagtat tacacaggta ttagccggtt tcacaaaaga agtagaggct gatgtatatg
      421 taatgacaca cacaacagca ccatttatta gcgaaaaatc tattgaaaaa ggactagatg
      481 ccgtgctgac aggagaatat gattctgcat ttgctgcaaa aaaattgcaa gatttcttat
      541 ggaaagacgg tgaaccattt aattatgaat tggacaatat tcctagaaca caggatttgc
      601 cgcctttata cgaagaaact agtggatttt atatttataa tcgcaatgtg atgaccgagt
      661 tgcatagaag aattgggaaa aaaccttgca tagttgaagt aggtgagatt gagtcagttg
      721 atattgatga agccgaggat tttatgattg ctgatgcgat ttataatcat cttattttac
      781 aaggtagtta agacaggggg aaggttagga tgaacaatgt acagatttta gattgcacac
      841 ttagagatgg gggctattgt aacaattggg aatttggatt tgataataca aaaaaaatta
      901 tttctggact tatagaatca ggaattgaga ttatagaatg tgggtttctc acaaataaaa
      961 cagtatacaa taaaaatgtt acaaaatata cgaccttaga agaattagca cagataattc
     1021 ctgaaaaaag agaaggaaaa ttatttgtag caatgatgaa ctatggcgaa tataatatag
     1081 aggatcttcc agattatgat ggttcatcta ttgatggaat taggattgcg tttcataaaa
     1141 aaaattgcaa tgaagcgttg aaattatgtc gtgagataaa gaaaaaaggc tacttagttt
     1201 ttgttcaagc tatggtatct ctcaattata cagatgaaga gtttttagca gtaataaaaa
     1261 atgtgaatca gtttaaacca tacgcttttt atattgttga tagttttgga atgatgaaag
     1321 gaaaagattt aactcgtctt ttttacatga ttgagcataa tctagaaaaa gatatacaga
     1381 taggttttca ctcacataat aacatgcagc ttgcatattc taatgcacaa aaacttataa
     1441 ccgtacaaac aaatcgaagc ctgattattg attcttcggt gtacggaatg ggaagaggag
     1501 caggtaatct taacacagaa ctatttgtag aatatttgaa tgataatgca ggaaaaaaat
     1561 atcaattaaa accattatta tatataattg atgaaatact aaatagtttc tatcagaaga
     1621 gttattgggg atattcatta ccaaactata tttcagcagt tcataatgct catcctaatt
     1681 atgcaagcta tctgtcatat aagaatactt taacaattga agcaatgaat gaaatattcg
     1741 aaatgatgga tgaagaaaaa aaggtatctt atgataaaca atatatagaa gatttatact
     1801 acaaatatat ggaaactggg aaaatacagg aggaatatat ggaagaactg agaaaatttc
     1861 tctcagacaa aaaaattcta ctgattgcac caggaaaaag ttctgtagat gaggtagaaa
     1921 gaataaaaga gtttgttacc aaagaagaag tcgtaataat tagtgtaaat tttgaatatt
     1981 ctcatataga tatagattac gtatttttaa gtaatttaag acgttttcat gaattaccga
     2041 aagaaagcag aactaaatgt attgttacat caaatataca agcatcggat gtatatttac
     2101 ggacgagtta taaaaagttg ttatctgaaa gggaagttgt aagagacaat gcaggattaa
     2161 tggcaattag atttttagcc gatatgaatg tggatgaaat attcttagct ggatttgatg
     2221 gatattcaca taatgaagat gagaattatg gtgagcaatc catggaaatt ataacaaaat
     2281 ttgcattatt agatgcaatg aacgtgcaaa tgagagatga attgtcagaa ttagctaaaa
     2341 aagtaaaggt gacattttta acggatccac g
//
LOCUS       DAZAOG010000238         2367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000238 DAZAOG010000000
VERSION     DAZAOG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.244"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..>2367
                     /gene="esp"
                     /locus_tag="ACHDLD_002785"
     CDS             41..>2367
                     /gene="esp"
                     /locus_tag="ACHDLD_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN3364438.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          860 a          372 c          484 g          651 t
ORIGIN      
        1 cttactagga aagtaattat taagggagtt gatttgaata atggttagca agaataataa
       61 gagagtattt ctagaaaaaa caaagaaacg agtattgaag tacagtatca aaaaattaag
      121 tgttggggtt gcatctgttt tgattggtgt tggtcttgtc ttgggaacaa ctgaactagt
      181 taaagcacaa gatgaaattt ctccaagcag cccacttgaa acagcgataa gctccgtaca
      241 agtaggtgac aaagtagctt cggggaatac ttttcaggag aatcctggtt atacgaaaaa
      301 ttataacttt tcggatttac aatttagtcc tcaagaacta actggagaca cactaaaagg
      361 caatacaatt ggatttgaag tttacggaaa acataatatt gcagcatcaa ctaaaaactg
      421 ggaaatccgt cttcaattag atgaacgatt agctaaatat gtagaaaaaa ttcaggtcga
      481 tccaaagaaa ggaattggat cttctagacg gacctttgta agaattaatg attcgctggg
      541 tagacctaca aatatttgga aggtcaatta tattcgtgca agtgatggat tatttgctgg
      601 ggcagaaaca accgatacgc aaaccgctcc taatggggta attacgtttg aaaagagcct
      661 agatgaaatt tttaaagaga taggcataga caatctcaag accgatcgtt taatgtatcg
      721 aatctacttg gtaagtcatc aagacgatga taaaattgta ccgggtattg atagtacggg
      781 ctacttttta acagattcag atgatttcta taatagctta gatgtatctg aaaataatcc
      841 tgaccagttt aaacatggat cagtaagtgc aaaatatgag aaacctaata ctcaaacaaa
      901 agatggttca ggatccacag gtgctaatgg agctataatc ttagatcata agttaacgaa
      961 aaattataat ttctcatatt catcttccgc aaagggcacg ccttggtatg ctaattacaa
     1021 aattgatgaa cgattagttc catatgtagc gggtatacag atgcacatgg ttcaggctga
     1081 caaagtaaca tatgatgttt cgtttgaatc cggaacaaaa gtagctgatt tagcaatcga
     1141 gcgacgggaa ggtcatgaaa attatggcat aggttcaata actaataatg atttaactaa
     1201 acttatcgat tttgctaatg caagtccacg tccagtcgtt attcgatatg ttttacaatt
     1261 gactaagcca ttagatgaaa tcttagaaga tatgaaagca acagcacaag ttgaagaaaa
     1321 taaaccattt ggtgaagatt tcatctttga ttcttggttg tcggatacga ataaaaaatt
     1381 aatccagaac acttatggaa caggttatta ttatttgcaa gatattgatg gtgatggaaa
     1441 ccctgacgat aaagaagaga gcggagacac gaatccatat atcgggaaac ctgaattaga
     1501 agaagtatat gatgttgaca caacagttaa ggggaaagta ttcatccacg agttagcggg
     1561 aacaggtcac aaagcccaac ttgttgataa agaaggtact gtattagcag aaaaaactat
     1621 cgctccaaat gaaaaagatg gggctccaat ttcagatact gtagaatttg aatttacggg
     1681 tgtagattca agtaaattaa tcgcgaaaga tgaattaaaa atccaaatcg tttctccagg
     1741 ttttgataaa ccagaagaag gttcaaccgt tattaaggaa tcaccaaaag cggttgataa
     1801 acaaaccgtg gtagttggat ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa
     1861 cttacctgaa gatgcagagt attcatggaa aacagagcct gatacttcta acgttactga
     1921 tagtacgaaa ggtattgtaa ctgttaagat cggaaatcga actttcgacg tggatgtaga
     1981 gtttgctgta aaagcttctc aagctatgga aaatgatgca acatacgtac ctataacaac
     2041 aactccagaa acgacagttc aaagtggaaa acctacattt gataaaccag atgttccttt
     2101 agctaaagat gccttttcaa tttcagatgt ctataataag tacttcggta atgcaagtgt
     2161 tgatgcaaac actggtattg ttacattcac tccagctaaa ggtgtaggag aatcggagcc
     2221 gattactgga acaattccta ttaaaattgt ttaccaagat ggttctgtag gcacgaccga
     2281 tttagcagta actgtaagta aagatattta tgaaaatcca ggagaaaaca ttcctgcagg
     2341 ctaccacaaa gtaaccttca ccgcagg
//
LOCUS       DAZAOG010000239         2241 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000239 DAZAOG010000000
VERSION     DAZAOG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.245"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..>2241)
                     /locus_tag="ACHDLD_002786"
     CDS             complement(151..>2241)
                     /locus_tag="ACHDLD_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN3364439.1"
                     /translation="AQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLGYLQIVNG
                     EEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAKDLLEIA
                     TKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYSAMVNKV
                     TADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASELPGVSTG
                     TDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLEKQYEDV
                     LQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRNYQTLIN
                     NGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPGSVVKAG
                     TLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSSNTYMIK
                     VALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPGISRSVD
                     YMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPHLVKAIY
                     GNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKPLASAKM
                     DLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDHINLTIA
                     KEIMDAYYDMFMAK"
BASE COUNT          572 a          501 c          411 g          757 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg c
//
LOCUS       DAZAOG010000240         2235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000240 DAZAOG010000000
VERSION     DAZAOG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.246"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2118)
                     /locus_tag="ACHDLD_002787"
     CDS             complement(34..2118)
                     /locus_tag="ACHDLD_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3364440.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          646 a          509 c          355 g          725 t
ORIGIN      
        1 cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt ttacatgcaa
       61 gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa atactgcatc
      121 tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta tcccaatagc
      181 aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga ttgcttctgc
      241 aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg cagcatcgct
      301 tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg catcgttgat
      361 tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct ctaaccgctg
      421 cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt gcttcccgat
      481 atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca caccagcctg
      541 ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa taacaagata
      601 accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt gacttaaagg
      661 aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt gtttgccatc
      721 aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg gtaaaagcgg
      781 acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact gttctgcact
      841 cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct ggatcgaaaa
      901 gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt gcgcaagcaa
      961 ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc cgataccgcc
     1021 aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca ctaaaaatgt
     1081 caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac
     1141 agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat
     1201 gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta ataatttgct
     1261 gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat ttttattgat
     1321 aaatccgctt aatactgttt caccaacata tacagattta gggactgatt ctcctgtaag
     1381 tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg gtacacgttc
     1441 accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca cggtcatcaa
     1501 tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt cgcgaatcga
     1561 ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat aaaatagcat
     1621 cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag caacagacat
     1681 aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct tcttcactac
     1741 atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg gttcattaaa
     1801 atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga acagcgtatt
     1861 tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg ttactttgac
     1921 atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag ccaaatgatc
     1981 ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt ccttgactcc
     2041 attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat ctaaaccttc
     2101 taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca tctcttcata
     2161 actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac
     2221 atctgcatcg gaaaa
//
LOCUS       DAZAOG010000241         2218 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000241 DAZAOG010000000
VERSION     DAZAOG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.247"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHDLD_002788"
     CDS             <1..365
                     /locus_tag="ACHDLD_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3364441.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACHDLD_002789"
     CDS             complement(721..1293)
                     /locus_tag="ACHDLD_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3364442.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHDLD_002790"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHDLD_002790"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN3364443.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2112..>2218)
                     /locus_tag="ACHDLD_002791"
     CDS             complement(2112..>2218)
                     /locus_tag="ACHDLD_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN3364444.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          685 a          457 c          337 g          739 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctagttggt tctgttgcaa agttttaaat aaagaataaa atcccttacg
     2101 gtatctatga tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac
     2161 ccgaagagag tgccttcttt tcgggttttc ttatataatc ctcgaatggc ttccatgc
//
LOCUS       DAZAOG010000242         2180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000242 DAZAOG010000000
VERSION     DAZAOG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.248"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHDLD_002792"
     CDS             complement(<1..914)
                     /locus_tag="ACHDLD_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN3364445.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHDLD_002793"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHDLD_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN3364446.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHDLD_002794"
     CDS             2006..2176
                     /locus_tag="ACHDLD_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364447.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          740 a          384 c          418 g          638 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag
//
LOCUS       DAZAOG010000243         2174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000243 DAZAOG010000000
VERSION     DAZAOG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.249"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>2174
                     /gene="acm"
                     /locus_tag="ACHDLD_002795"
     CDS             28..>2174
                     /gene="acm"
                     /locus_tag="ACHDLD_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN3364448.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFL"
BASE COUNT          854 a          348 c          423 g          549 t
ORIGIN      
        1 ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg
       61 ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc
      121 agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga
      181 ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat
      241 tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa
      301 atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg
      361 caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt
      421 gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg
      481 acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct
      541 agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg
      601 tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa
      661 atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt
      721 gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc
      781 gctacattta atttttcagt aactgataat tacatagaaa taactatacc aaaaaacttt
      841 gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata
      901 aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga
      961 gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt
     1021 agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg
     1081 actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg
     1141 acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta
     1201 aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa
     1261 ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag
     1321 gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt
     1381 tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca
     1441 aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat
     1501 agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc
     1561 ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc
     1621 atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg
     1681 acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt
     1741 gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa
     1801 atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat
     1861 aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg tagccatata
     1921 acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct
     1981 caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt
     2041 agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca
     2101 agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct
     2161 gggatattct taca
//
LOCUS       DAZAOG010000244         2150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000244 DAZAOG010000000
VERSION     DAZAOG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.250"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1539
                     /locus_tag="ACHDLD_002796"
     CDS             136..1539
                     /locus_tag="ACHDLD_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFN3364449.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            1560..1844
                     /locus_tag="ACHDLD_002797"
     CDS             1560..1844
                     /locus_tag="ACHDLD_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFN3364450.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          748 a          315 c          449 g          638 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 catattatta tttgtcaaat taagcgagac aaaactttcc atttacgtaa
//
LOCUS       DAZAOG010000245         2146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000245 DAZAOG010000000
VERSION     DAZAOG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.251"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..506
                     /locus_tag="ACHDLD_002798"
     CDS             3..506
                     /locus_tag="ACHDLD_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364451.1"
                     /translation="MNFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDS
                     GLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNR
                     KIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNP
                     KTYDILA"
     gene            complement(567..932)
                     /locus_tag="ACHDLD_002799"
     CDS             complement(567..932)
                     /locus_tag="ACHDLD_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN3364452.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1008..1253)
                     /locus_tag="ACHDLD_002800"
     CDS             complement(1008..1253)
                     /locus_tag="ACHDLD_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3364453.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1323..1577)
                     /locus_tag="ACHDLD_002801"
     CDS             complement(1323..1577)
                     /locus_tag="ACHDLD_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364454.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1741..2064)
                     /locus_tag="ACHDLD_002802"
     CDS             complement(1741..2064)
                     /locus_tag="ACHDLD_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364455.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          808 a          364 c          374 g          600 t
ORIGIN      
        1 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
       61 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
      121 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
      181 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
      241 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
      301 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
      361 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
      421 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
      481 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
      541 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
      601 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
      661 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
      721 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
      781 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
      841 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
      901 tttgaaatca atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt
      961 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     1021 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     1081 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     1141 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     1201 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     1261 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     1321 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     1381 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     1441 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     1501 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     1561 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     1621 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     1681 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     1741 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     1801 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     1861 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     1921 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     1981 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     2041 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     2101 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaa
//
LOCUS       DAZAOG010000246         2140 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000246 DAZAOG010000000
VERSION     DAZAOG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.252"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..545
                     /gene="ahpC"
                     /locus_tag="ACHDLD_002803"
     CDS             3..545
                     /gene="ahpC"
                     /locus_tag="ACHDLD_002803"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN3364456.1"
                     /translation="MEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCPTEL
                     EDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISRIFD
                     VLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGEVCP
                     AKWKESGETLKPSFDLVGKI"
     gene            555..2084
                     /gene="ahpF"
                     /locus_tag="ACHDLD_002804"
     CDS             555..2084
                     /gene="ahpF"
                     /locus_tag="ACHDLD_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN3364457.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT          692 a          369 c          463 g          616 t
ORIGIN      
        1 aaatcgaaga atttaatgca catgcttatc atcaaggaga atttttagaa gtcagtgaaa
       61 aagatcttat ggggaactgg agtattcttt gcttctatcc agctgatttt acttttgttt
      121 gtcctactga attagaagac ttacaagaac aatacagtac attgaaatct ttaggtgttg
      181 aagtcttttc ttgctctacc gatacccact ttactcacaa agcatggcat gatacatcag
      241 atgctatcgg taaaatcgaa tacgtgatga tcggtgatcc gtctcatcag atttctcgta
      301 tttttgatgt tttggatgaa gagcaaggat tagctcagcg aggaacattt attattgatc
      361 cagacggaat cgttcaagct atggaaatca atgcagatgg aatcggtcgt gacgctagtg
      421 cactgattga taagattcgc gctgctcaat atgtacgtac ccacccaggt gaagtttgtc
      481 cggcaaaatg gaaagaaagt ggcgaaacat tgaaaccaag ttttgatctt gttggaaaga
      541 tttaggtggt ggatatgtta gacaaagaaa ctaaacaaaa tctcgaacaa tatttagcac
      601 tgattgaatc tcctattgtt ttttctgtta gtttggataa tagtgaaaat agtcaaaaac
      661 tagctgaatt tacgaaggaa atagcagaaa tgtctcctaa aatctcttgg gaaaaaagag
      721 attcgatgcg cacacctagt ttttctatca atccagttgg caaagaaagt ggaatcgttt
      781 ttgctggtat ccccctcggt catgaatttt cctctttttt actggctatg ttacaaatta
      841 gtggacgggc accgaaaatc agtgaatctt tgagtcttaa aatcaaaaaa ataacacaaa
      901 cattgcattt tgacagttat gttagcttga cttgtcataa ttgccccgac gttgtacagg
      961 cattgaatat attagctgta ttaaatccaa atattactca tacgatgatt gaaggtggga
     1021 tgttccaaaa agaaatcgat gataaaaaaa tcatggcagt gccaactgtg tttttaaatg
     1081 gagaagaatt tagtagcgga cgcatgacga tcgaacaaat tgttgaaaaa atcactggcc
     1141 ctttgattga agaagagttg tttgaaaaag aaccttatga tgtattagtg attggcggtg
     1201 gaccagcagc tagtagtgca gctatctatg cagcacgtaa aggaatccgt acaggacttg
     1261 tcgctgattc tttcggcgga caagtcgttg aaacactagg gattgaaaat atgattggta
     1321 cctcttacac agaaggtcca aaattgatgc agcaagtaga aagtcatgtg cgtagttacc
     1381 ctatcgacat tatgatgaat caacaggccg tttccttaaa taaagagcaa cattttatta
     1441 atattggctt agctaacggt atatcactta aagcaaaaac cgccatcatc gcgacaggtg
     1501 cgcactggcg ttctatcaat gtaccaggcg aaaaagagtt taaaaataaa ggaattgctt
     1561 attgcccaca ctgtgacggc cctttattta aaggaaaaga gattgctgtt atcggtggtg
     1621 gtaattctgg tgttgaagca gcaatcgatt tggctgggtt agcaaaacat gtatatgttc
     1681 tagaattctt accagaatta aaagcagatc aaatcttaca ggataaacta gtctctcttg
     1741 cgaatgtcac tgttatcaaa catgcagcga cgaaagaaat cagcggtacg aatcaggtag
     1801 aatctctctc ctatatcgat cgccgtacga tggagatgca tacactagct gtttcaggag
     1861 tattcattct agtcggctta ctccctaata cggattggct agatggaacg atcgaaatga
     1921 atccaagaaa tgaaatagtg accgagaaaa atggcgcaac gaatatcccc ggcgttttcg
     1981 cagctggtga ttgtacagat agtccataca aacaaatcat tgtttcaatg ggttcaggag
     2041 ctactgcagc tttaggagca ttcgattatc tgatgcgcac ttgagctatg aagattgtaa
     2101 aaagtcagag agcggaaaaa aggatggtga caagaatttt
//
LOCUS       DAZAOG010000247         2069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000247 DAZAOG010000000
VERSION     DAZAOG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.253"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1309
                     /locus_tag="ACHDLD_002805"
     CDS             164..1309
                     /locus_tag="ACHDLD_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN3364458.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="ACHDLD_002806"
     CDS             1446..1790
                     /locus_tag="ACHDLD_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN3364459.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT          725 a          350 c          432 g          562 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaat
//
LOCUS       DAZAOG010000248         2060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000248 DAZAOG010000000
VERSION     DAZAOG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.254"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHDLD_002807"
     CDS             <1..1008
                     /locus_tag="ACHDLD_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364460.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHDLD_002808"
     CDS             1025..1174
                     /locus_tag="ACHDLD_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364461.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHDLD_002809"
     CDS             1171..1617
                     /locus_tag="ACHDLD_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364462.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHDLD_002810"
     CDS             1619..1756
                     /locus_tag="ACHDLD_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN3364463.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2060
                     /locus_tag="ACHDLD_002811"
     CDS             1794..>2060
                     /locus_tag="ACHDLD_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364464.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIK"
BASE COUNT          752 a          357 c          425 g          526 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa
//
LOCUS       DAZAOG010000249         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000249 DAZAOG010000000
VERSION     DAZAOG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.255"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHDLD_002812"
     CDS             <1..378
                     /locus_tag="ACHDLD_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFN3364465.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHDLD_002813"
     CDS             426..1826
                     /locus_tag="ACHDLD_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN3364466.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZAOG010000250         1993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000250 DAZAOG010000000
VERSION     DAZAOG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.256"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHDLD_002814"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHDLD_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN3364467.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHDLD_002815"
     CDS             852..1040
                     /locus_tag="ACHDLD_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN3364468.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHDLD_002816"
     CDS             1053..1610
                     /locus_tag="ACHDLD_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3364469.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHDLD_002817"
     CDS             1642..1884
                     /locus_tag="ACHDLD_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3364470.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAZAOG010000251         1932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000251 DAZAOG010000000
VERSION     DAZAOG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.257"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACHDLD_002818"
     CDS             144..542
                     /locus_tag="ACHDLD_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN3364471.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="ACHDLD_002819"
     CDS             526..1221
                     /locus_tag="ACHDLD_002819"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFN3364472.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1805..>1932)
                     /locus_tag="ACHDLD_002820"
     CDS             complement(1805..>1932)
                     /locus_tag="ACHDLD_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HFN3364473.1"
                     /translation="LEKIATEAKTLDTEFMDKTIRKVMKKDNVTIKDPYVKNIFK"
BASE COUNT          713 a          283 c          365 g          571 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt
     1681 tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa
     1741 taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat
     1801 ttgtttattt gaatatattt tttacataag gatctttaat agttacattg tcttttttca
     1861 tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa
     1921 ttttttctag ct
//
LOCUS       DAZAOG010000252         1931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000252 DAZAOG010000000
VERSION     DAZAOG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.258"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..1545
                     /locus_tag="ACHDLD_002821"
     CDS             79..1545
                     /locus_tag="ACHDLD_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3364474.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          593 a          331 c          396 g          611 t
ORIGIN      
        1 acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa caaattttta
       61 tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt tgcgttaatt
      121 gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca gcaaggagta
      181 tcagttgtca cctcttggct attgattttg gccatttatt ttattccata cgcattgatc
      241 gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc atgggtcgaa
      301 aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg ggtcgtccat
      361 attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg ggcagtccaa
      421 ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat ttcacttatt
      481 atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa agtgattggc
      541 ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt agctgtaacg
      601 gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca agtgaaaaca
      661 tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt atttgcagtt
      721 ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc aaaggaattt
      781 cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt aggctcagtc
      841 gcaatgggta tgctgtttgc tagtgataat attcctaaag acttgatggc aaatggggca
      901 tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt gatgatcatt
      961 tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat tgatgcacca
     1021 ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct acgtaaacgt
     1081 aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt aggaatcatt
     1141 attttgttgc cgatttttgg tatcaaagat atgaatgaat tagtaaaatg gttgacgaac
     1201 ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata tatgatgttg
     1261 aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc aaaaatcggt
     1321 tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct aggaatggtg
     1381 ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt tacgaatatc
     1441 atcacaccaa tcgtcttgat cttattaggt atgatcttgc cagcaattgc tcgtcgagac
     1501 aaaaacaaaa aaatcactga accagaaaca gtggttcagc cttaaaatag aaatacttgt
     1561 tacaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt
     1621 gcttctcaaa tttttgtgaa gtttcggctt gtttttcgaa gaagctactt ctggaacacc
     1681 gtgaatcaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
     1741 atagcccctc atatttttcg tttggctggg cttattaccg cagatgcaag ctttggaaca
     1801 ccgtttaagg aagaggttgt aaaatcagcg atttgacctg agtattagag aaacaaacgg
     1861 aaaaatagct cctcatattt tgccgctttt tgagctaatg ctgaaggtca gctgattgaa
     1921 caccatttat t
//
LOCUS       DAZAOG010000253         1919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000253 DAZAOG010000000
VERSION     DAZAOG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.259"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..733)
                     /locus_tag="ACHDLD_002822"
     CDS             complement(<1..733)
                     /locus_tag="ACHDLD_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3364475.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSS"
     gene            complement(744..1412)
                     /locus_tag="ACHDLD_002823"
     CDS             complement(744..1412)
                     /locus_tag="ACHDLD_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN3364476.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1542..>1919)
                     /locus_tag="ACHDLD_002824"
     CDS             complement(1542..>1919)
                     /locus_tag="ACHDLD_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3364477.1"
                     /translation="ENIDIEKSWRENFNELDPFINYIFENKRIFDLILFQSAGTKYQD
                     IVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSK
                     EKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          692 a          314 c          247 g          666 t
ORIGIN      
        1 tcgaactatt aaaaatgtca aatttcctta atgcgttatt gttttcagtt tttaacaact
       61 gaatagttgt agtttttaca gttctatttt taacaaatag aacttgatca attaattctt
      121 tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt tttaaatcac
      181 aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac ggttcatcca
      241 atataattaa tttagagttt agcgataaac tacatccaat agaaattttc tgccgttcac
      301 cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat aattcagcta
      361 attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc atcgaaaata
      421 ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga cttacgtagc
      481 caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt tcaaataaaa
      541 tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt aactttaata
      601 gtgtggattt cccactccca gaagaaccag taatcaatgt aagactgcct ttttttattg
      661 aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa gaaacattgt
      721 taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat agtaacggaa
      781 aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact tcttttcaca
      841 ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat catagttgca
      901 cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa aaaagacaag
      961 cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg ataagtcgga
     1021 aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg tattttttct
     1081 aaagaggata ataattcatt gctagtatag acactaacca acaaccatga gatcattacg
     1141 accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt aaaaaactct
     1201 atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa
     1261 taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac
     1321 cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa
     1381 gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca
     1441 ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa
     1501 aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag taattcaatc
     1561 cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa
     1621 tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt
     1681 tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa cttatctaga
     1741 caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc ttgatatttg
     1801 gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa aatataatta
     1861 ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttc
//
LOCUS       DAZAOG010000254         1899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000254 DAZAOG010000000
VERSION     DAZAOG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.260"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHDLD_002825"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHDLD_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN3364478.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="ACHDLD_002826"
     CDS             661..915
                     /locus_tag="ACHDLD_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3364479.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="ACHDLD_002827"
     CDS             916..1233
                     /locus_tag="ACHDLD_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3364480.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          655 a          290 c          354 g          600 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acttccctgc
     1681 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1741 tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata
     1801 cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc gtggtgctag
     1861 ttaattttaa tatcctaaag aatatctcaa agttccttc
//
LOCUS       DAZAOG010000255         1886 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000255 DAZAOG010000000
VERSION     DAZAOG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.261"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..812
                     /locus_tag="ACHDLD_002828"
     CDS             <1..812
                     /locus_tag="ACHDLD_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN3364481.1"
                     /translation="KKIKKLASTQQLQSRKSKLLFLALVFEDQSYVIIDQSGHPVEYS
                     PAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKELANCWVGLPEE
                     RFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRS
                     QIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIREGKPVMIGILK
                     VLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            892..1866
                     /locus_tag="ACHDLD_002829"
     CDS             892..1866
                     /locus_tag="ACHDLD_002829"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN3364482.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
BASE COUNT          625 a          328 c          429 g          504 t
ORIGIN      
        1 ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc
       61 tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc
      121 cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga
      181 agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc
      241 ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg gtgggattgc
      301 cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta
      361 cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc
      421 caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta
      481 gccagatcca accactagga cttcctacga ttccttttca ggttagtaca gggatttcta
      541 gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc
      601 agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac
      661 ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag
      721 gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca
      781 gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt
      841 ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat
      901 aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa
      961 aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat
     1021 ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa
     1081 tcaacgaatg cacctgtcaa tgactactat atggaactac tgatcatgat cgatgcaatg
     1141 aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa
     1201 gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa
     1261 gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc
     1321 tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa
     1381 ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt
     1441 gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat
     1501 gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta
     1561 gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac
     1621 ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt
     1681 attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa
     1741 aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc
     1801 aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc
     1861 gaataaacaa aaaaaagagg gaaaga
//
LOCUS       DAZAOG010000256         1852 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000256 DAZAOG010000000
VERSION     DAZAOG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.262"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..716
                     /locus_tag="ACHDLD_002830"
     CDS             150..716
                     /locus_tag="ACHDLD_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3364483.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            861..948
                     /locus_tag="ACHDLD_002831"
     tRNA            861..948
                     /locus_tag="ACHDLD_002831"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Ser,seq:cga)
     gene            1282..>1852
                     /locus_tag="ACHDLD_002832"
     CDS             1282..>1852
                     /locus_tag="ACHDLD_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN3364484.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          649 a          279 c          356 g          568 t
ORIGIN      
        1 ccaaattttt ttaattttgc aaaaaaaagt taaagaagac tgaaaaaaaa tttaaaatat
       61 aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata gggtacaata aaacaacata
      121 tactgcagta aagaagtggg ggagttttaa tgagaagaaa agtctatacc aaggatcaaa
      181 ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt taaaggattt actgcgagaa
      241 atattgctaa aaagatgggg atttcgacac agccgattta tctggaattc aaaaacatgg
      301 aagatttaaa aaatacgttg ctggattcta tctttgaaca actaaaagaa gatgttttcc
      361 ctattgaacg tactggtgat aaaatcattg atctcggttt gaactatatc tactttgcaa
      421 aaaagcatca tagtctttac atggcattgt acattgatga acatggggac ggaaagaaaa
      481 tgcatgattt ttcatacaat tatttccgtg aaatagttaa agaagatgaa agatacaatc
      541 aattatcgga tgaatatgtc gatgctctgc atactggtac atgggtagca gtgactggct
      601 tagcgtcatt gatgtcatca aacatcatgc atccaacaga agaacaaatc attgcactga
      661 tcaaacggac aattgactcg attttagcat tagataatcc tgaaataatc agatagtgtt
      721 ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa actgtcaatg gacttttttg
      781 ctaaaaaaag cgatttctgt tatttatcta ttagaaatag gcttgacatt tcgtgaaaaa
      841 agtttaaaaa tagtacttac ggaaggttgg cagagtggta atgcaccgga ctcgaaatcc
      901 ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt accttcctta tatcaacgat
      961 tagaggttgt gaagaagctg tcctgcatca agtaataaga acggccaaac aaaaatagct
     1021 tttcctattt tttcgtttga ttgggcttat taccgcagat gcaagctttt gaacaccgtt
     1081 tgttcggaat aaggggctgt gacaagactt ttgtcacagc cccttattgt tgtactttta
     1141 acctacaata ttttttgatt ccccctgagt actatttttt ataaatgcta gtaaatcaat
     1201 cttctttggc ctttttttga taaaaaaaat actagatgta agataaaagt gtaaaaacga
     1261 tttgttttag gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa agaccattat
     1321 tttggaagga attttgatta tgagatgtct tataatgaag tggtcactat tactccaaga
     1381 aattatcgtt tcgattttag aaaggtaaaa aatttagata aacattatgc aatgatagga
     1441 atagcagctg gggtatccaa ctatccactc tattatgagg ctacaaatga aaaagggttg
     1501 agtatggcag gactgaattt tcctggcaat gctgattata aagaattaca agaaggaaaa
     1561 gataacgttg caccatttga gtttattcca tggatactag ggcaatgctc aaccatagac
     1621 gaagctaaag aattattggc tactatcaat ctagtcaata tcgattttag tgaaaaacta
     1681 cccttgtcac ctttacactg gttattagct gataaagaaa aatcgatagt aattgaaagt
     1741 atgaaagatg gacttcattt atatgataat cctgtcggtg tgttgacaaa taatccgcca
     1801 tttgattatc aattatttaa tctgaacaat tatcgctcgc tatcaaatgg aa
//
LOCUS       DAZAOG010000257         1820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000257 DAZAOG010000000
VERSION     DAZAOG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.263"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..728)
                     /locus_tag="ACHDLD_002833"
     CDS             complement(114..728)
                     /locus_tag="ACHDLD_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3364485.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1334..1711)
                     /locus_tag="ACHDLD_002834"
     CDS             complement(1334..1711)
                     /locus_tag="ACHDLD_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364486.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          585 a          367 c          288 g          580 t
ORIGIN      
        1 acaaaaaaag tagtcgttgt taatcctcat taataatcgt tttctttttt tcaaacgctc
       61 catttttatg tggaacatta tttttttaag gaactttgtc aacatgtgga taactatttt
      121 ccacttgtgt cctggactag ctgttttgct gtaaaataca aaacttgctg cagcaaaaag
      181 atcgttgcaa gagaggctgt tccgcagatc gctggatcta gatttggcaa agtcaagact
      241 gcatcatgta aattggcgtg gttgtccaaa accaaatcag caaattcaaa taatcgcaaa
      301 ccactgctat gttgcgattt gatacttcgt gacaaatcaa atccagtaac aatgattaat
      361 ggatgaccat tttctttgat ccactgagct agttcgatga ttgccgggtt tcttccttca
      421 ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa gctgatcaat aattgcctgt
      481 cctgcacctt ctagcgtttc atagacacct ccggctggat tttcaatcaa tatacttggt
      541 acaagatccg gggtttgatc ccaaaattca aatgctacac cacttagtgt gcggctagca
      601 tatatctgca atctaccacc ttgattaatt tgtttcgcga tccaagtgcc agcttgaagc
      661 atcagcggct cttctatcct taatttctct tccaattggt gataatagtc aaaataacat
      721 aaactcattt atctctcccg cctagctata cttgacatga atagaaacag ctgcctaatc
      781 taaggaagta cataaaaaaa cagtagttag acagtaacac atgtggtctt tgctgtttca
      841 aacacaaaaa aggcattgaa cgaatacgcc caataccttg acatagcaaa taaaacaacg
      901 tgcagaccat ggaaaatatc tacatagggg tagaattttc accctagatt aggtacaata
      961 aattaggtcg atgcactgca ttgactatgg caaaatgatc ggtcagggtg tatgtgtgtg
     1021 actggtcatt ccctgattct tagcgccaac taagggtcag ggagcctttt tttattcagc
     1081 tgtttctatc tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa gaaaatacaa
     1141 aaaaacattg gtatgattta tatatatata aataacataa aaaaaggaat ggcttatccc
     1201 gatacaacaa gcattccttt aaaaaagatt cttttatata agtgaaaaaa gcttttttcc
     1261 accaattaaa taaatctctc atctcaatag tttatccatt attttatctt tttttgcgta
     1321 tacctactaa atattaccgt cttcttctcg ttcttctact ttgttcgaga gaatctgtac
     1381 tagaaaaagt agttgtatgt ctaccttttc tgcctatccg agactttctt ttcatatatg
     1441 ggatataaat gattagatac agtacagcaa aaaataaagc aaatacgcaa gcttcccaac
     1501 tacctaacgc caattcaacc atcaaggtag caaccccaac ggatgctgca taccagataa
     1561 aaatcagaaa cgtttctttt tttctgacca tgcgaaacat gataaataga ctaataagca
     1621 atagtggcaa cacaaatatc caattgcttc cttccatcag ccacttgagt atatcaagca
     1681 gcgactgtgc aacagcgact aattttccca tagtgaacac acaccttttt ttataattaa
     1741 aaaattcgcc tttttattct actatgaatc ttctggcttg gctttcattt ctcttatgta
     1801 atcattttct tttatacaac
//
LOCUS       DAZAOG010000258         1790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000258 DAZAOG010000000
VERSION     DAZAOG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.264"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..162)
                     /locus_tag="ACHDLD_002835"
     CDS             complement(<1..162)
                     /locus_tag="ACHDLD_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN3364487.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEY"
     gene            371..583
                     /locus_tag="ACHDLD_002836"
     CDS             371..583
                     /locus_tag="ACHDLD_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364488.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            745..1263
                     /locus_tag="ACHDLD_002837"
     CDS             745..1263
                     /locus_tag="ACHDLD_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFN3364489.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1270..1782
                     /locus_tag="ACHDLD_002838"
     CDS             1270..1782
                     /locus_tag="ACHDLD_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364490.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          686 a          233 c          273 g          598 t
ORIGIN      
        1 atattcttgc acccaacgat aaatcgtcgt atgagaaacg ttaatgccac gatcatataa
       61 gatttcttga acttcacgat agctaaggtt ataacgaaga tagtagccca cggctacaat
      121 aatcacatcc tgctgaaatt gctttccttt aaaatgattc atcgtcattc ctcctgctat
      181 ctttttctat tattctacct tatttgatag tagatttaaa actttgcaac agaaccctaa
      241 aaacactata tattccgaaa agtgagtatc tccattcata aaataatcaa catatttaaa
      301 aaatctaatt agctttttta gtcaatctaa agttctttat tctatgatat aatgttactg
      361 tagaaagttt gtggcggttt ggcaaatcca attgaaggag cgtggtgcct atgggcttat
      421 tagcttatcg gacgtactct gtagaaagga aatgcctgat ttgttctcat tggatcaaat
      481 cattgctatt atcggagcag cagcagtagt tattactgcc gtagcgaaag ctagcgattc
      541 gattttagct tggttaaagt tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg
      601 gcgaggtaga cgagctctaa agctttattt acatgccatc cgctttgaac ggactacact
      661 gggcatgtga cagcatgccc tttcatttat attatatctc attcttttat ataatgaaag
      721 taaataaatc atggaggata aaatatggat aaatttaata gattaacatt aataaatcag
      781 tttgaaattt taaaagcttt aaatcctaac gaagaaaaat actatgctga aaaaatagaa
      841 atactaacag aaggttacga atatcactat agtgaaatat ttgaaaatat ttctgatcca
      901 ttgccagaag aagattcgcg gtttgttcta gacatattaa atatgtatag agatattact
      961 tttagtaaaa ataaattaaa agatataaat tcaattgata attattatat acaatttaaa
     1021 ggttttgact ttaatagctc taccgaattt aagttagcac tttatgcaca attttttatt
     1081 gaaaaattaa ataggtttca agaaatagtt gaagatagtg attttaacac ttttaatagc
     1141 cataaaccga tgagaggtac ttatataaaa tttctcgaaa attataatga tttaaaatct
     1201 accaataatt atcaatttgg aaacttgtct tatgagatga taagtaaagt tctaagtttg
     1261 tgatgataaa tgaaattaaa tagtataaat gatttatttg ctattatagg tgttattgct
     1321 aacatacttt tttcgtgttt gaattttaga tttactcaaa agaacaaaaa gaacaaactc
     1381 agtatagaac aaatgaaaaa agacattgaa actgatttaa aaaatgacca taaagaaaga
     1441 agaaaaattg aagaatatac ttctttaatg gaaaagtgta aagcaactga tgcaattttt
     1501 agctttttaa gaaaaagtga tgagataatc acacgtgaaa atatagattt agaaaatgag
     1561 attagtaaac ttgaatctat cttttccgat ataaattctc ttaagtattt aatcgtcgaa
     1621 gaaaattcgg atagcttttt agaatttgaa aaatatctaa cagatcttat agataataga
     1681 aataatgaat taatgagaca aaacattgca agattcctta ctaaattagt ttctgaatgg
     1741 aaaagtatta tttctcaaaa tatggataaa ttaagatatt aaggatgtta
//
LOCUS       DAZAOG010000259         1770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000259 DAZAOG010000000
VERSION     DAZAOG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.265"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..313
                     /locus_tag="ACHDLD_002839"
     CDS             <1..313
                     /locus_tag="ACHDLD_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364491.1"
                     /translation="FSFYSKYINKLARTSLGVYILHENHYFIDGMKSRGLIWDGIFGL
                     GKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNLCKLMNHVINIY
                     P"
     gene            336..1361
                     /locus_tag="ACHDLD_002840"
     CDS             336..1361
                     /locus_tag="ACHDLD_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN3364492.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            1610..>1770
                     /locus_tag="ACHDLD_002841"
     CDS             1610..>1770
                     /locus_tag="ACHDLD_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3364493.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          627 a          213 c          376 g          554 t
ORIGIN      
        1 tttttcattt tacagtaagt atataaataa acttgcgaga accagtttag gggtatatat
       61 tttacatgaa aatcattatt tcattgatgg tatgaagtct agaggcttaa tatgggatgg
      121 gatatttgga ttaggcaaaa ggtttttaga tagggatttt tggatttggt ttataattgc
      181 agttgtagtt gtgtgcataa tttgcatggc tatcgatgtt gtaagagaaa aatttgaaag
      241 acagattttt aaaattaaga gtgtaaagaa cctgtgtaaa cttatgaatc atgttataaa
      301 tatataccca taattaataa atgaggagaa aagaagtgaa agttatatca ttcggagaat
      361 tattattgag acttgcagca cccggttatt caaagttatt tcaaagagat agtttggaga
      421 ctacattctg cggaggggaa gctaatgtag cggtatcctt agcaatattt ggatatcaat
      481 cagaatttat aacaaagctt ccggattcag atgttggata tgcggcagct aatgctttgc
      541 gttattttgg agtaaatgtt gataatatta tatttggaga gggacgcatg ggactgtatt
      601 atttagaaaa aggagcagct cagagaccat ctaaagtaat ttatgacaga aaattttctg
      661 caatttcgtt agctgatagt gatgaattta agtggaagga gatcctaaac gatgcaaatt
      721 ggtttcattg gacagggata aatccagcat tgtctgagaa tctctttgag acgtccttag
      781 atgcatgtca ggctgccaaa aaaagaggta taacaatttc ttgtgattta aattatcgat
      841 ccaagctttg gacatcagag gaagcaaaag aaaagatgga acagatacta ccattggttg
      901 atatttgtat ttgtaatgaa gaagatgcag aaaaagtgct aggaattaaa gctgagcata
      961 caaatattaa tagtggagaa ttaaataaag atggctatgt gcaggtggct agggagattc
     1021 acaagaagtt tggatgtagg tatgtagcta caactctgcg tagaagttat tcggcaagta
     1081 gaaatggttg gagtgcaatg ttatacgaag cagaaaaaga taaagcgtat ttttccacag
     1141 aatacaacat acaaattgtt gatcgcgttg gtggtggaga cagcttttct gctggtataa
     1201 tatatgcgtt atgtgaaaat atgtccggac aaaaggctat tgaatttgcg actgcatcta
     1261 gttgtttaaa acatactata gaaggtgatt ttaatcgaac tacaattgag gatgtatata
     1321 atttaatgaa tagcggtgga aatggtcgcg tgttgcgtta gtcaacatat aaagataaat
     1381 cacagtaatt tttatggtga ttatctgtct tagtagtgat tagaataacg tgaaaataaa
     1441 ttgttgagcg tttatactgt tttgaaaaat aaatttaaca tattgaaaaa tacaaatata
     1501 tacgtatatt ataaagcgcc gattgtaaaa ttaagctaga caactaaaac agtcatttgt
     1561 gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtgcaaa taacctatac
     1621 ctatcttaca acggatgaac ttgtaataat agagtcttat ttgaaaatta accaatctgt
     1681 tgctaaaact gcgcattgct tgaatcgttc aagtcaaacg atccataaag tatatctgtt
     1741 cttcaagcaa agaaaatcag ccttagaata
//
LOCUS       DAZAOG010000260         1767 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000260 DAZAOG010000000
VERSION     DAZAOG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.266"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..>1767
                     /locus_tag="ACHDLD_002842"
     CDS             43..>1767
                     /locus_tag="ACHDLD_002842"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFN3364494.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGIS"
BASE COUNT          644 a          224 c          353 g          546 t
ORIGIN      
        1 actttttgga aaaatcttac taataagtag aggagaaaga aaatgactat aaaagtagac
       61 ttacgaaaaa aaccatatca tttagatgaa gatgctattt cttggattga agagacgatt
      121 gatgctatga ctttagaaga aaaaataggt caattatttg ttaatatggg atctagtcgt
      181 gataaaaatt acttaagtag tatggtcaat cagtaccata ttggagctgt acgatataat
      241 ccaggaactg ctgatgaggt atatgatcaa aactatatcc tacaaacaga aagcaaaatt
      301 cctttattaa ttgctgctaa tacagaagca ggtggtaacg gagcttgtac agatggtacc
      361 gaagttggtg tagaagtaaa aattggtgca acaaatgatg cgaaatatgc ttatgaatta
      421 gggagagttt ctggaattga agcatcggct attggatgta actggagttt tgctcctatt
      481 gtagatatta atagaaattg gagaaatcct atcatttcta gtcgatcttt tggacaaagc
      541 cctgataaag ttttagaact tagtttagct tatatgaaag gtattcaaga gagtggaatt
      601 ttgcctgcag ctaaacattt ccctggtgac ggtattgacg aacgagatca acatctatct
      661 tttagtatta atagtttaag tacagaagaa tgggataatt cttttggaaa agtatactca
      721 ggcttaatag aagctggatt accttcaatt atggcaggtc atatccatat gccagcttat
      781 tcaaaacatt ttaatcttga tctgaaagat gaagatttac ttcctgcaac tttatcaaaa
      841 gaattaatca ctgacttact tagaggaaaa ctcgggttta atggattagt agttactgat
      901 gctagtcata tgttaggaat gactggggca atgaaacggt cagaattatt gcccacttct
      961 attgcggctg gatgtgatct attcctattt tttaatgatc cagatgaaga ctttaattat
     1021 atgatggaag gttattacaa aggaattatt tcagaagagc gtttacgtga tgcattaaga
     1081 agaattttag gcttaaaggc taaactaggt ttgcataaaa aagaaaagaa taaaattttg
     1141 ttaccgaaac aagaagctat ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa
     1201 gaggtagctg atcaagcaat tacactagtt aaagataaac aagatatttg gcctattgta
     1261 ccagaaaaat ataaaaggat tttactggta gatgtacatg gggtaaaagg tgggtttggt
     1321 gaagtagttg ctggaaatag acctcaggcc attgatttat tgaaacagat tttgtcagca
     1381 aaaggatttg aagttgaaaa atacatttca cctgaagaag aattgaaagg attatcgcaa
     1441 gaagaaaaaa ttaaaaatat agctggaata tatgctgcta agagaccaat caaagattta
     1501 actgaaaaat atgacttgat tattaatata gctaatgtta acccaggtac agttcaaaga
     1561 attgtttggc cagcaagtaa aggtactccg gatattccat tttatattca tgaaattcca
     1621 acaatttttg tatctgtaca gtgtccgttt catttagctg atgtgcctca agtaaaaact
     1681 tatatcaata cttatgatgg aaaacaagaa actttagaaa tattagtgga caaactaatg
     1741 ggcctttctg aatttaaggg aataagt
//
LOCUS       DAZAOG010000261         1750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000261 DAZAOG010000000
VERSION     DAZAOG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.267"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACHDLD_002843"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACHDLD_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN3364495.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          482 a          306 c          348 g          614 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa
//
LOCUS       DAZAOG010000262         1739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000262 DAZAOG010000000
VERSION     DAZAOG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.269"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACHDLD_002844"
     CDS             complement(<1..189)
                     /locus_tag="ACHDLD_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3364496.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKY"
     gene            complement(186..839)
                     /locus_tag="ACHDLD_002845"
     CDS             complement(186..839)
                     /locus_tag="ACHDLD_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3364497.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1127..>1739
                     /locus_tag="ACHDLD_002846"
     CDS             1127..>1739
                     /locus_tag="ACHDLD_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN3364498.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          555 a          315 c          235 g          634 t
ORIGIN      
        1 atattttatt tcttttccat tcatattact agaaatttca tttgccatat tatgaatctt
       61 ttcttttctc gtctccaaat atgttttagg aaagataaaa aaaattaatg aatgaactaa
      121 tataattatt attccaagaa cagaaaatat ttgtatgaac atttttggaa atatctttaa
      181 ttttttcatt ttctctccaa tttatatcct acatttctta tagtagttat acagtctaat
      241 tgtaattttt ttctaagttc ttttatatat acatctatta ctcgatcata aggagtttct
      301 tcgctatctt tccatacata atcaataatt tgcattcttg ttaacacttg tccattatta
      361 tccaataaac attttaatac ttctagttct tttgcattta catctatttc ttcaccattt
      421 attttagctg tatagctatt aaaattaacc gatagatcct tatattcaaa cttctctaaa
      481 tgtccataat tcttcttaat taaagaatct attctagctt ttaagaccgg caatgaaaat
      541 ggcttttcta catatccgtc tactaaatta gtaaatgcat caattttata ttcttcatca
      601 ctaaatgcag tcaagattag aattggtaaa ttgcttttct ttctaatttc tttcaacact
      661 tctaaaccat ttataaaagg tatctgaatg tctaagataa ccaaatttat atcgctatta
      721 aattttgaca aggcttctct tccatcttta gcttgaataa cagtatatcc aaattctgaa
      781 agatattcac ttattccctc tcttatcata ttatcatctt caacagttaa tattttcata
      841 tatacctcca cttaagccta tacactagat attatacagc atattatggc tctataatat
      901 ctagtgttgg tgacaccttc tgttgccaat actttttctt ttttctaata aatgcaaaca
      961 gacaccttga aatcctttag aataaagggt tctataatat tttgtgttgt tggaaaatcg
     1021 aaggcgattt tctactaaca ctttttcttc gtttcaactc aaaaagagac tcacttttgg
     1081 taaaatttag tcgaccaaaa catcacccta ccaaaggagt cgctctatgg ataatcatac
     1141 tagaaaatta ctcggattaa cagataaaca tttatttttt gatgaagaat ggctgattga
     1201 aaaagaatat aaaggcgttc aagcgcagtt tattaaaggg agattggatg actcaccttc
     1261 ccattgtgaa cactgtggat cgattcgtat tatccgcaac ggttcctata cgacacgtac
     1321 tcagatattg aaagtcaaag aaaagttgac tattttagaa ttaaaacgta cccgttttct
     1381 atgctatgat tgtgggcaga ccttttctgc gaaaacggac ttagttgatg aacatcatca
     1441 actaacaaag gaactgaagc aggcgattct catagaactg tacgaaaatc aatcacgtaa
     1501 actaatcgct aagaagtact tcgtctcgga tgggacggtg acacggatct tgcgtgaagc
     1561 aacgaagcat taccagccac gtatgaactt ccttccgacg gtgttgtgta tggatgagtt
     1621 caaatcaatg aagtctgttt ctggttccat gagttttatt tgtgttgatg ggacaaccca
     1681 tcagctattt acgatccttg aagatcgcag actttacaaa ctaactcaat acttcctgc
//
LOCUS       DAZAOG010000263         1725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000263 DAZAOG010000000
VERSION     DAZAOG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.271"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1714
                     /locus_tag="ACHDLD_002847"
     CDS             191..1714
                     /locus_tag="ACHDLD_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN3364499.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT          638 a          288 c          376 g          423 t
ORIGIN      
        1 tagaggttga ttgcaaaaat atcgtcaatc attcatataa actttctcat caattaaaat
       61 aagaaaacgc ttgacagttc ttttaaccat atgataaatt aaatcctgtt gctaaaacgt
      121 aactattacg gtttgcgaaa agcgaatcgg aacattcaaa tgaaagaaga tgagaagagg
      181 agtgtcataa atgaaacgat tgataggtat attggccatg ctggttttag caggtatgtt
      241 aactgcttgc ggggcaagtg ggaaagccga ggacagtaaa gaaaaattga gcgtgatgac
      301 gaccttttat cccatgtatg attttacaaa agcaatcgtc ggggatgaag gagaagtaga
      361 gttattgatt ccagcaggga ctgattccca cgattatgaa ccttctgcta aagacatggc
      421 aaagatccaa gatacagata tctttgttta taatgatgaa aacatggaaa catgggtccc
      481 cgcaatccaa aaaacattac aagaaggcaa tgtccatacg ataaaagcga cagaaggtat
      541 gcttttactt ccgggaagtg aagaaggaca tgatcatgac catgaacacg gggaagaagg
      601 ccatacacat gagctagacc cacatgtatg gctagctcca agtttagcaa tcaagcaggt
      661 agcaaacata cgtgatcagc tgattgaagc ttacccagaa aaacaggaag tatggacaaa
      721 aaatgcagca gcctataccg agaaacttca agcactccat cagctttatc aagaaacctt
      781 taagcaagca aaacaaagat cttttgtcac gcaacatgct gcattcaact atctagcgtt
      841 agaatatgga ttgaatcaag tatcgattgc tggtctttct tctagtgaag aaccgtctgc
      901 ggcgagaatt gctgaactga aacactttgt taaagaacat ggaatcaact atatttattt
      961 tgaagaaaac gccaaagata gtattgccag aacgttagca aacgaagcag gtgtatcttt
     1021 agaagtattg aatcctttgg aaggattgac gaatgaacaa atagaaaatg gagaaaacta
     1081 tctttctatc atggaagcaa acctcgaagc gttgaaaaaa acgacagaga cagaaaaccc
     1141 gctagaagat actttgaccc ccaaaaaaga aaagagcgtc tacaacggtt attttgaaga
     1201 cgaggatgta caagatcgct ctctttcaga ttgggcagga acttggcaaa cagtagatac
     1261 gtatgtagaa gatgggactt ttgatcctgt ttttgaatat aaagaaaaat tgaatcagga
     1321 taagacagca caagaatata aagaatatta tacgaagggc tatcaaacag atattgcttc
     1381 aatcaagatc agtgagaaag agatggcatt tacgtttaag gatggaacaa cgaaacgttc
     1441 aaattatcgt tatgtaggga aagaaatctt gacctacgag gcaggaaacc gtggcgtaag
     1501 gtatctattc gaagcagaag ataaggaaag tggcgctttt agatatattc aattcagtga
     1561 tcacacgatt gcaccggctg actccgaaca ttttcattta tatgcaggaa atgaaagtca
     1621 agaagtattg ctgaaagaaa tggagaattg gccaacattt tatccagata caatggatgg
     1681 acgtgaaatt gcacaggaaa tgcttttgca ctgataggca aaaaa
//
LOCUS       DAZAOG010000264         1707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000264 DAZAOG010000000
VERSION     DAZAOG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.272"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(502..1254)
                     /locus_tag="ACHDLD_002848"
     CDS             complement(502..1254)
                     /locus_tag="ACHDLD_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3364500.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1269..>1707)
                     /locus_tag="ACHDLD_002849"
     CDS             complement(1269..>1707)
                     /locus_tag="ACHDLD_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3364501.1"
                     /translation="LKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFD
                     CIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGI
                     TTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          556 a          297 c          223 g          631 t
ORIGIN      
        1 caaaaggact tgcagcgctg tagttggtgc tgcaggagga cctatttcgg ccgttacagg
       61 aggtattggt aatgtacttg tcggatgtgc attaaagcct gttcctgcaa gttaaattta
      121 taaaaacagg aaagtaatta tctcaaatcc tatcaagtac aatcatagga ttgcacttga
      181 caggacaaat tttttaacta aaaaatgaaa aacactttct aaaaattagt agaatttgcc
      241 aaatctttcg catcttccac catacattta attttaattc ccctttatgg tacttaataa
      301 aaacaagaaa agaactattc atttggccct tttttggttc cagaccatag aaatattcct
      361 ccagtaacta atattgctgt tatgcacgta actaataaca gctataaaaa ttggtacaac
      421 tttaatagga ttcagaaaaa tttgcactag tgaaggaatc tattttatta gaaaaatgca
      481 atctccctaa agaaaaaacc actaaacagt gctttctttc aacttctcct taattttttg
      541 tgctttccct aaagtaaagt ttctggccaa ctctgaatca aaatatagtg aacgtttttt
      601 aaaatctatt tctttaacgt taaagatatt tactaaacac gctcgactaa ttcttgttaa
      661 cataggatat ttcttttcaa gctcactaat tcttccataa aattcgtact gcccatcttt
      721 agtacacaat gataatctat gtggtaattt tgaagcttct acaaaataaa tgtcatttat
      781 atcaaaagta aatgtttgag aacctatgga aaaaacaaat gcttgattcg taacctgttt
      841 tgttgcatct atcctttctt gcgctaataa caataactca acaatctcgt ttcgatactc
      901 atctagtgtt tgatcttttg ttacaaatcc taacgtttca actcttcttt ttattgttac
      961 gggtaacatc tcatcatgag tagttgtaaa aatgattttt gcttgaacat catattttct
     1021 gattacttct gctaattcta taccgttaac ttcatgattt aaatcaatat ctaagaaata
     1081 tattccttgc tttggtcgga attgtttgag gtatttttta acttctaacg gactttgtgt
     1141 tttcaatact atcttaaata ctttatcgtg aaaaagaatg aaattatcaa taattcgttc
     1201 taactgattt aattgtataa tttgatcttc acataatatt attggataac tcataaatcc
     1261 tccttccctc aaaacaacaa aataacttga gtagtaaatt gatacggatc gttttgatag
     1321 ttcacaaaca tattgggatt ctttttattg atttcctcta tactagataa acctaaccct
     1381 tgatgtccct tttttgttgt tattcctttt ttcattagag tatctataga gagtagtatc
     1441 tctctacaac tattcgatat cgaaatttct atctgtttat catctcggta aatagctaga
     1501 gacagttctc tatcttgact ctctatagat gcttcaattg cattatcgag aataattcca
     1561 agcaccctaa tacagtcaaa tattggaaca ggaacttcaa taatagtact tggacattcg
     1621 aatttacaac aaatcttatt gttatttgct tggtatagct tcgctatcaa caaacttttc
     1681 aaatattcat tttttatatt ttttaaa
//
LOCUS       DAZAOG010000265         1688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000265 DAZAOG010000000
VERSION     DAZAOG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.273"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            219..575
                     /locus_tag="ACHDLD_002850"
     CDS             219..575
                     /locus_tag="ACHDLD_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3364502.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            585..1484
                     /locus_tag="ACHDLD_002851"
     CDS             585..1484
                     /locus_tag="ACHDLD_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3364503.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          572 a          292 c          348 g          476 t
ORIGIN      
        1 taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt tccgttcaag
       61 aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc ataataaacg
      121 aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag ttgcatctga
      181 tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa gtgacaaaag
      241 aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta ttagatatcg
      301 aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat acggcatttc
      361 gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct gtagtggctg
      421 atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg gatgatccat
      481 ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca agcggcttga
      541 gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga aaattggcga
      601 tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg atgcagcttt
      661 ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat acggctaata
      721 aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta tctacgacat
      781 ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg gaccattttc
      841 cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc gttcttttag
      901 ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag gcaatcgtac
      961 gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca gatctcgagg
     1021 tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa gcaaaaaaac
     1081 ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg tatcaatctg
     1141 ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca ttcggagaat
     1201 tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat cgtcgctttg
     1261 aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat tattatgaat
     1321 ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat gatcctatga
     1381 aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa acacaacatt
     1441 tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc attagtaatg
     1501 tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat ccatttatcg
     1561 tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat cggatgattt
     1621 gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata atcacaaaaa
     1681 aaaatata
//
LOCUS       DAZAOG010000266         1672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000266 DAZAOG010000000
VERSION     DAZAOG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.274"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..513
                     /locus_tag="ACHDLD_002852"
     CDS             139..513
                     /locus_tag="ACHDLD_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3364504.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(627..1256)
                     /locus_tag="ACHDLD_002853"
     CDS             complement(627..1256)
                     /locus_tag="ACHDLD_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3364505.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1390..1462)
                     /locus_tag="ACHDLD_002854"
     tRNA            complement(1390..1462)
                     /locus_tag="ACHDLD_002854"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1427..1429),aa:Thr,seq:ggt)
BASE COUNT          587 a          242 c          284 g          559 t
ORIGIN      
        1 atccaagtct acactctgag atgtaagaaa tatcataaga tatttcttac atctttttgt
       61 tttgagcagg actttagata attttattaa tatatgctag catgcttttg taagaaaaag
      121 aaagagggcg tattagttat gtatcttaaa attacggaac aagcacaaga aagattacaa
      181 aaatatattg atgaaggcgc aacagttata ttggatttag atgatggcgt tggggaatat
      241 tctaaaatgg gtgtttgctc tttagatact agttttcgtt tacttcttct tgataaagaa
      301 cagagaaaaa atgattataa actccaatta gatagtgata ttggggatgt ttatataaaa
      361 gattattcaa aaatgtacat ggatgaacat atgacccttt ctttagatcc acgtctaggt
      421 gtatttactc ttgaaagccc aagtggttct ttagacagtc atgttcaatt agtagatcta
      481 cgaccagaaa aattctctgg aataaaacaa taactttctc tctattgtcc ataaaactag
      541 tctcaatcaa gctgtattga aaaaacaaga caacattctt accatggtaa ataaaaagac
      601 gacacatttt gtcgtctttt tttaaattaa ctattttcga taaaagatcg catagcttgt
      661 gccattattt ctggcttttc tctattcaat tcatgttttc cgtttttaat aaagagtagc
      721 cggccattcg ttgaaagttt atataaacgt tttgctgctg gtttattcgc atgatctttg
      781 gtaccgcata aaatcaattt aggaagtcgt gatgtacgaa gctgtttttt gcagtctaat
      841 gtttcaagtg aacgaagtag ggtaagggtt tgttttttag atagacctaa tttttcaaat
      901 atcttagaag gtacgcagta aaaataaata ttctggaaag taagcaataa gcgattagga
      961 gactcaaatt gtggagcgac taaaacaacc ttttttaaat atggagaagg agttgcaaga
     1021 tagtttaaag caataacagc gcctaaagat aatccacata aaataaaagg ttcctttatc
     1081 tgattcaatt gtacttcgat gtatttggtt aagtcgtcta aagtagcagt gtctgtgctg
     1141 tttaaatgat tgaacaatga aagacttttg caagaataac tattgggtag ataagagatt
     1201 acatctttcc atgattgttc gtcttggcct agaccatgca aaaaaacaat tttcatattg
     1261 ctctcctttt tatagttatc atatagactt tctttgtact attaaaatat aaacattcat
     1321 ttcttacgtc ataaaataaa aaaacagcgc agacacattg ttaaaccaac atgttgcact
     1381 gtttaaggaa gccacttgcc gggcttgaac cggcgacctc ttccttacca tggaagcgct
     1441 ctaccagctg agctaaagcg gcattatcat taatacaaat aataggataa cgaatattaa
     1501 tgtatttgtc aacagaattt tttcgaaaaa atgtgtttct ctaataggaa aacagatttt
     1561 agaaaaaata aaaataacaa aaaatgcgaa cggtttcttt gataatataa taaaaaaatt
     1621 caaaaaaagt attgcgttat ttagaagaaa ttgatattat attcatgtac tt
//
LOCUS       DAZAOG010000267         1672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000267 DAZAOG010000000
VERSION     DAZAOG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.275"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1622
                     /locus_tag="ACHDLD_002855"
     CDS             75..1622
                     /locus_tag="ACHDLD_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN3364506.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          560 a          305 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DAZAOG010000268         1666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000268 DAZAOG010000000
VERSION     DAZAOG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.276"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1418
                     /locus_tag="ACHDLD_002856"
     CDS             <1..1418
                     /locus_tag="ACHDLD_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN3364507.1"
                     /translation="YEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTID
                     TNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTD
                     NVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRS
                     NADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVV
                     TYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTT
                     YTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIV
                     TGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPT
                     NVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISG
                     GLILLGTLGLLGYKNRKKENE"
     gene            1552..>1666
                     /locus_tag="ACHDLD_002857"
     CDS             1552..>1666
                     /locus_tag="ACHDLD_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFN3364508.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEI"
BASE COUNT          662 a          251 c          356 g          397 t
ORIGIN      
        1 aatatgaacc aacagtagaa ggcgaaaaag tagaagttgg gggcacagta gatctaacag
       61 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      121 gcggtacaat tgataccaac acaccaggta attacgaagg tgtcattgaa gtaacgtatc
      181 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctgacg
      241 ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc acagtagatt
      301 taacagataa tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta
      361 ctcccggcgg tacaattgat accaacacac caggcaatta tgaaggtgtc attgaagtaa
      421 cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt
      481 ctaatgcaga taaatacaat cctgaaggac aaaaagtgac tacagactta aataaagagc
      541 cagatgcatc tgagggaatt aagaacaaag aagatttacc aaaaggaact acttatgctt
      601 ggaaagagaa agtagatgtt agcacagctg gaaataaaaa aggtactatt gtagtgacat
      661 atccagatgg atcatctgat gaagttgaag tagacgttac agtgacagat actcgttccg
      721 atgcagataa atataatcct gaaggacaaa aagtgactac agacttaaat aaagagccag
      781 atgcatctga gggaattaag aacaaagaag atctaccaaa aggaactact tatacttgga
      841 aagaaaaagt agatgttagt acagctggaa acaaaaaagg tactgtagta gtaacatatc
      901 cagatggatc taaagaagaa gtagaagtta ctatttctgt agtagataaa aaagcaccga
      961 ataaacctca agttgatcct attacagagg gtgaccaaat tgttactggt aaaactgaac
     1021 caaatgcaga ggtgacagtt acattaccag acggaagtca acaccatggg acagctgata
     1081 aaaatggtaa ttttacagtt aaagttccta aattagaggc agggacaaaa gtgatagtaa
     1141 ctgcaactga tgaatctggt aatactagtg aacctactaa tgtagttgtt tcttccaaca
     1201 aaaaagacag tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca
     1261 gcaatcaaga caaaaataga ggcaaaagcc agtcctcaaa agtttttcct aagacgggag
     1321 agagtgacag taatatcttt actattagcg gtggtttaat actgttagga acgttaggat
     1381 tattagggta taaaaatcga aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc
     1441 tctgagaaga ggaagaatct ttttcttcct ctttttgctt ttttatgatg ggaaataagt
     1501 gttagttaaa gatgaatata ctttttatag aaaggaaaag aatgtaatta agtggaaaaa
     1561 atagtaatta ttggttcaaa ccattcaggg ctagccgcag ctcgatttct aaacttaagc
     1621 gattcaaatt atgaagttat tctaatcgat aaaaaatcag aaatta
//
LOCUS       DAZAOG010000269         1645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000269 DAZAOG010000000
VERSION     DAZAOG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.277"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..323
                     /locus_tag="ACHDLD_002858"
     CDS             <1..323
                     /locus_tag="ACHDLD_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364509.1"
                     /translation="YGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEK
                     TINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVE
                     YGII"
     gene            310..>1645
                     /locus_tag="ACHDLD_002859"
     CDS             310..>1645
                     /locus_tag="ACHDLD_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3364510.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIR"
BASE COUNT          530 a          214 c          250 g          651 t
ORIGIN      
        1 gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa
       61 atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta
      121 tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata
      181 cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt
      241 ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt
      301 tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc
      361 tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt
      421 gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca
      481 attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac
      541 tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta
      601 attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg
      661 aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat
      721 atccagtctt attttacgat taactttaac cattttaaat atttgatttt atcttttata
      781 tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat
      841 aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt
      901 tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct
      961 ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc
     1021 gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt
     1081 agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca
     1141 tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat
     1201 tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca
     1261 aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta
     1321 ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa
     1381 acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg
     1441 attttcatac ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt
     1501 ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca attatttgac
     1561 acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat acttcatatt
     1621 ttaatcaatc aaatgttgat tcgtt
//
LOCUS       DAZAOG010000270         1629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000270 DAZAOG010000000
VERSION     DAZAOG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.278"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACHDLD_002860"
     CDS             202..1077
                     /locus_tag="ACHDLD_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN3364511.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACHDLD_002861"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACHDLD_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT          584 a          291 c          255 g          499 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgtta
//
LOCUS       DAZAOG010000271         1609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000271 DAZAOG010000000
VERSION     DAZAOG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.279"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHDLD_002862"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHDLD_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHDLD_002863"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHDLD_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          568 a          242 c          214 g          585 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataag
//
LOCUS       DAZAOG010000272         1600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000272 DAZAOG010000000
VERSION     DAZAOG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.280"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..194)
                     /locus_tag="ACHDLD_002864"
     CDS             complement(<1..194)
                     /locus_tag="ACHDLD_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364512.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVCFLIS"
     gene            complement(208..438)
                     /locus_tag="ACHDLD_002865"
     CDS             complement(208..438)
                     /locus_tag="ACHDLD_002865"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN3364513.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(532..870)
                     /locus_tag="ACHDLD_002866"
     CDS             complement(532..870)
                     /locus_tag="ACHDLD_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364514.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          545 a          282 c          200 g          573 t
ORIGIN      
        1 aagctaatta agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt
       61 gtgtagctac ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac
      121 tgcattaaag ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg
      181 tcaaaatttt tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc
      241 ttctacttca gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga
      301 tccttctctg taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc
      361 tttccactca ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt
      421 tcccttttct accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta
      481 catctagtaa attttcatca atttctatat cataaagtat ttcactagtg tctacatcca
      541 caatatatac tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc
      601 gtcactttca tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag
      661 ctctaagcct actgtatttt ttactattga gtcattattt accaccataa cttcgtactg
      721 tatataccat cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt
      781 ctccggaaca atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc
      841 atgttcctca cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa
      901 aataaacact acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc
      961 caatcacaaa ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac
     1021 tacaactatt catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga
     1081 tacttgtaat agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa
     1141 ttccaataca caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt
     1201 attctagcaa tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta
     1261 aagttaactt tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga
     1321 aatggagtga gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata
     1381 atggttattt gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca
     1441 aaacctttga ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca
     1501 aaggtgttaa aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat
     1561 tgaaaaataa ctacagataa tacttttata aacgtacgtg
//
LOCUS       DAZAOG010000273         1575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000273 DAZAOG010000000
VERSION     DAZAOG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.281"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..1531
                     /locus_tag="ACHDLD_002867"
     CDS             65..1531
                     /locus_tag="ACHDLD_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN3364515.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          438 a          291 c          331 g          515 t
ORIGIN      
        1 agttaaattt ttcggtaaag atgtataata ttttctgaaa atttaaagaa ttggggtata
       61 gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat tgtcaacttt
      121 gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat tacttatcta
      181 ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa cagcattagc
      241 tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg gatgggtatc
      301 tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca ttatgcttgg
      361 ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga tggcattgat
      421 tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac atctttattc
      481 aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg tcaacttagg
      541 ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact atcatttagg
      601 gttctcttta gctgctattg ggatgttctt tggattatta caatacatca tccaaggacg
      661 caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg aagaacgtaa
      721 aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat tcggcggggc
      781 acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg tattggggat
      841 tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa ctgctgaaga
      901 aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt tctggtcatt
      961 acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac aatctacgct
     1021 atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt tcatctttat
     1081 tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac catctacagt
     1141 agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat tgatgttgcc
     1201 gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg gaagtttctt
     1261 catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga caacaaaatt
     1321 agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg atgcttcttc
     1381 acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat ctgcttactt
     1441 cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg tgaaagaacc
     1501 aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag gaattgctta
     1561 tttgcagcag ttcct
//
LOCUS       DAZAOG010000274         1567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000274 DAZAOG010000000
VERSION     DAZAOG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.282"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1034)
                     /locus_tag="ACHDLD_002868"
     CDS             complement(12..1034)
                     /locus_tag="ACHDLD_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFN3364516.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            complement(1040..>1567)
                     /locus_tag="ACHDLD_002869"
     CDS             complement(1040..>1567)
                     /locus_tag="ACHDLD_002869"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3364517.1"
                     /translation="VMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLIIGTGDMEEE
                     IKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFF
                     SEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKI
                     LESKYYELFELIQRG"
BASE COUNT          495 a          283 c          170 g          619 t
ORIGIN      
        1 acctctaata tttataataa tttgttttct accaacatcc cataaaaagt aactttatca
       61 tctttttgct ttttaaaaga ttgtttccat gtcaacttaa acagtaaagt atctttcttt
      121 aataagttcc atactttttc atcatatata ttatttaaat ttttaatcaa ttcgtcacat
      181 aatggattgt tactaggtat tttatcaaat gtctgtgcta tttcatgatt tattctctgt
      241 gctaaaacaa tcatataatc tatcatcaaa taatccacca tcatatcatt atttttccaa
      301 taatttaaaa aaaagtctct aattatagca aacatccatc tagactcttg attacatgct
      361 aatgaataac ccgcaaaata tcctgatgct acggatgcgt gagcataatc aggcctccta
      421 atagaccaaa tatcgatatc aaaatactca tctggtattg gttttgtaca ataaaatgtg
      481 gcgtctaacc acaaacctcc atgttttgct agcaaagata atcttaataa atcagaataa
      541 tttgttctcg taatgattcc tttgtttttc ttttcttcca cccattttgg tatatcaaca
      601 tattgtctat aattatcttc tgttaaaatt ataacagtat gtttccctgc atttcgtttt
      661 attgatttta cacaagcttt tacaatttcc ggcgattgat ccaatccctg ccaccaacaa
      721 acccaaatgc agttagaata tggtgatttt gattgattaa ttatttgatt ttgactataa
      781 tcatatgttt tcaaaaaatc tgaaaaagtc ttttcataat acttcagcat tacctcatgt
      841 ttttttaata aatgttcctt ctgtttttta ctttcattat atccattacg attcatgact
      901 tgaatatcaa ttttagcttg taacgtggca aatgcttcct tccaagatgt tatctttgca
      961 attcgtcttg ttgcattcaa ttcttcggat atgcgatgta taattctttt tatacttgaa
     1021 tgtttctgtg tcatattctt taacctcttt gtattaattc aaataactca taatattttg
     1081 attctaagat tttgtatgct gtttgaatat tataatctgc ctttattaaa atcgaacgtt
     1141 ctgaatctcg atgagagata tctacatttg caatctctct cgcccattct tttgccgatt
     1201 gatttaaact tatgaaattg gtcttatcac tgaaagcaac ttctgtagga actttctccg
     1261 aaaagaaaca cggtaaatct gcaaattgag cttcaatacc cactaacgga accccttcaa
     1321 ataatgatgg caacattaat acgtcaaatg cttgataaaa tttttctata tcatttctat
     1381 ttcctaaaaa tttaatatca tcatctatgc cacttttctt tgcataattt tttatttctt
     1441 cttccatatc accagttcca ataattaata atacggaatt attcttaata gcgtgaaatt
     1501 ctttaaaaat ttcaatcaaa aaacgatgat ttttagaata tgaaattctt ccaatatgtc
     1561 ccattac
//
LOCUS       DAZAOG010000275         1563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000275 DAZAOG010000000
VERSION     DAZAOG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.283"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          590 a          179 c          316 g          478 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caa
//
LOCUS       DAZAOG010000276         1493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000276 DAZAOG010000000
VERSION     DAZAOG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.285"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..761)
                     /locus_tag="ACHDLD_002870"
     CDS             complement(93..761)
                     /locus_tag="ACHDLD_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3364518.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="ACHDLD_002871"
     CDS             895..1182
                     /locus_tag="ACHDLD_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364519.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          552 a          234 c          251 g          456 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tat
//
LOCUS       DAZAOG010000277         1470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000277 DAZAOG010000000
VERSION     DAZAOG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.286"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..723
                     /locus_tag="ACHDLD_002872"
                     /pseudo
     CDS             109..723
                     /locus_tag="ACHDLD_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1093..>1470)
                     /locus_tag="ACHDLD_002873"
     CDS             complement(1093..>1470)
                     /locus_tag="ACHDLD_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN3364520.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          534 a          238 c          216 g          482 t
ORIGIN      
        1 ctatttagag tttaccctaa atagacaaaa atataacatt gaaaaattta gagttctaac
       61 gtatgattag ggtgtattat aaacgttata gaaaagtagg gaataaattt gaaaattgct
      121 tatattagag tttcatccat cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag
      181 tttggcgctg aaaaaatttt tatcgaaaaa caatctggag cgacgattag tcatcggccg
      241 attttccagg aagctttaga ttttgtcaga gagcaagata tttttattgt agaagccatt
      301 gatcgacttg gacgcaatta tgacgagatt atcgattcag tgaattatct aaagaagaag
      361 aatgtgcgac ttattatcac cagtttaccg atcatggctg aagcaattgg aaatcctctc
      421 ctagataaat tcatcaaaga cttaatcatt taaatctcag ccatgattgc tgaacaagaa
      481 cgtactgaat cgaaacgacg ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc
      541 tataaaggtc gtcctaagtt atacagtgca gacaccaaag atcctcaacg tcgccttgtc
      601 tataggagta tcgttcagga tcttgagaat ggggttgcta tctcaaagat tgcaacagac
      661 tacaatgtga ctaggcaaac aatttaccga ataaaaaaag aaatcgacca actaattgta
      721 tgatttcttt tttgaatcac gtaattatta attagctttg tcatcaatac agacaatcat
      781 tttagattca tctagctaag ttgattacat ttatgttaaa aagaatttag ttaacataaa
      841 tataattgat gcataaatcg tccttattta aaggcattta acattgacaa cttagtataa
      901 tgtatcttat gtaaactaat aagatttgaa ttttatttta tacacactcg ataatagatc
      961 aattttatgg tcccccgcat ttacgattgc acaagagaaa cataaagtcg acctattagc
     1021 atttcttttc aattcaaaca ttaaaaaagt ttatacctct gctactaaaa aatgaaatta
     1081 tctatttaaa atttaaaacg gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg
     1141 tattggagct ttgtatttct ccataataac agatatcttt ttttcatttt ttaaaaaata
     1201 ccttttaaat ttcttatagt cctctgaaat caaaatatag cgtgatggat actcatcaaa
     1261 agaagaatcg tattcttcct ttaaaattcc cgatttactt aatttttcta gtacatcaaa
     1321 tactctatca ctcaagtaac tccttaaatt gttatcactt tcccattgaa ctacttttct
     1381 ataagttgaa ttatcagttg aaaagataga attttcatcc ataaacgcat atattagcag
     1441 caatctttca tcattgtcaa attctattgg
//
LOCUS       DAZAOG010000278         1453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000278 DAZAOG010000000
VERSION     DAZAOG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.287"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHDLD_002874"
     CDS             complement(<1..625)
                     /locus_tag="ACHDLD_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN3364521.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHDLD_002875"
     CDS             complement(784..1050)
                     /locus_tag="ACHDLD_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3364522.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHDLD_002876"
     CDS             complement(1040..1396)
                     /locus_tag="ACHDLD_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3364523.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAZAOG010000279         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000279 DAZAOG010000000
VERSION     DAZAOG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.288"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHDLD_002877"
     CDS             105..1406
                     /locus_tag="ACHDLD_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3364524.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAZAOG010000280         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000280 DAZAOG010000000
VERSION     DAZAOG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.289"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..364
                     /locus_tag="ACHDLD_002878"
     CDS             104..364
                     /locus_tag="ACHDLD_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364525.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(726..965)
                     /locus_tag="ACHDLD_002879"
     CDS             complement(726..965)
                     /locus_tag="ACHDLD_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364526.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1251..>1440
                     /locus_tag="ACHDLD_002880"
     CDS             1251..>1440
                     /locus_tag="ACHDLD_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364527.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSS"
BASE COUNT          502 a          192 c          257 g          489 t
ORIGIN      
        1 tttttatttt tttataaaat cacaagtgtt attgattttc acaatcatat tttaatggta
       61 ttatcatttt gaataatgaa aaaacattaa ggatggatga aatatgaata acgaaactat
      121 cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag tggttggaga
      181 agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc atgacgtcga
      241 tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc acgtttatga
      301 gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata ctttagtttc
      361 ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga aggcacttta
      421 tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc ctagagaatc
      481 tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc cagaactgtc
      541 ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa tcatttcaaa
      601 aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa
      661 aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg aagaagatga
      721 agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt ctttaccctg
      781 ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt tttctcattt
      841 gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat gttctttgat
      901 gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa cagaattctt
      961 ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc agtattaggc
     1021 aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt aatacatata
     1081 tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt attaacgatt
     1141 atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg tatgatatta
     1201 tctagaggta atttttttgg gtatttaaat agttgctagg aggatagctt ttggattttt
     1261 acagtaattt tatcttaata atagcaatat tattacttct aaatatttgg ttttttgata
     1321 agagtagaaa tgcagggata ggttttagaa ccaaacgaag tacgtcttct gaaaaaaagt
     1381 gggtatattc acagactatt ttttacggag gggttatttc aatcagttta ctttcttcaa
//
LOCUS       DAZAOG010000281         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000281 DAZAOG010000000
VERSION     DAZAOG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.290"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHDLD_002881"
     CDS             complement(150..1337)
                     /locus_tag="ACHDLD_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN3364528.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZAOG010000282         1385 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000282 DAZAOG010000000
VERSION     DAZAOG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.291"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1204
                     /locus_tag="ACHDLD_002882"
     CDS             35..1204
                     /locus_tag="ACHDLD_002882"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3364529.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          499 a          252 c          228 g          406 t
ORIGIN      
        1 tctgtaaaaa agaaaggaga ctaaataaac tattatgaaa aaaggaaaac atgctaaaaa
       61 aagaaaatat tcatttttaa tgatcgttgt cgtgttgttt agccttagtg cgttctctac
      121 aggaactatt cttggatggc aattttacaa taatgaacac tctcagatag ttgcccatgc
      181 tcaatcagct caaaaaaagt tgatactgac taccaatgaa caacagctcg ttgaaaaaat
      241 cgatcaacta ttaagaaaaa atgattatat tggctcaatt tacgtaaaga aaaataatcg
      301 catcatcatg gaaaaaggct atggctatgc gaataaactc acgcaacaac caaatgatcc
      361 ttctctttat tatcagattg gttcggttca aaaagcattg actactcttt tgattttaaa
      421 acaagtcgag cgtgggcacc tttctttaga tactaagctg gctcaattct atccacaaat
      481 tgctggcagc cagcaaatta ctatccgtga cttgctgtat atgcgctcag gcttgcaccg
      541 aactgcttct ccaaccatac cgatgactga tgaagaagtc gttcaatttg cgattcagca
      601 cttaaaattt acagattatc agacctctca ttatgattca ttgaacttta ccatattgac
      661 tggaattttg atcaaattaa caaatcaacc gtatgaaaaa ttattgaccg atgatatcat
      721 cgaaccttta ggattacagc atacaaaatt ttatgaccaa gtcaaaaatt catccaatca
      781 tgcactatcc tatcaaatgt ctaaagagga tgattacttt aaaccactga cagaatctga
      841 aacagagatc cataacgaat tagggactgg taatatcagt atgtccgtct atgatttaaa
      901 tacgctgttt acacaagttt tatctggaaa actagtttcc aaagacttac tcttctcatt
      961 atggcgtgaa aatgcgaatg gacatccata tagtggtggg gtatatagcg gagataatta
     1021 tatcctgtcg caaggaaata tcgactcttt tgatacatct gctatattta gcaaagattc
     1081 acaggatgct atcatcatgg aaagcaatat aaaagctgac aaaactatca aactgcctac
     1141 tgctgatcta agaaaccaaa tctatgacct gatggaagga actactaagt tggcaaatag
     1201 ctgatatgat gaaaagatag caaatactac ttctttgaat agcatttgct taaaaaaagt
     1261 actcgactaa acgtaggaaa gagtgtggga caaatctaaa aatcaatttt tgctacagac
     1321 tttaattcca aataaacaga acagaaagag gtgttcttct ttctgttctg tccaaattat
     1381 tctaa
//
LOCUS       DAZAOG010000283         1366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000283 DAZAOG010000000
VERSION     DAZAOG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.292"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1366
                     /locus_tag="ACHDLD_002883"
     CDS             82..>1366
                     /locus_tag="ACHDLD_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN3364530.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DAZAOG010000284         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000284 DAZAOG010000000
VERSION     DAZAOG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.293"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHDLD_002884"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHDLD_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFN3364531.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZAOG010000285         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000285 DAZAOG010000000
VERSION     DAZAOG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.294"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHDLD_002885"
     rRNA            <1..1355
                     /locus_tag="ACHDLD_002885"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZAOG010000286         1339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000286 DAZAOG010000000
VERSION     DAZAOG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.296"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACHDLD_002886"
     CDS             <1..1309
                     /locus_tag="ACHDLD_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFN3364532.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DAZAOG010000287         1330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000287 DAZAOG010000000
VERSION     DAZAOG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.297"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..663)
                     /locus_tag="ACHDLD_002887"
     CDS             complement(175..663)
                     /locus_tag="ACHDLD_002887"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3364533.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(685..1287)
                     /locus_tag="ACHDLD_002888"
     CDS             complement(685..1287)
                     /locus_tag="ACHDLD_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3364534.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          420 a          312 c          191 g          407 t
ORIGIN      
        1 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
       61 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
      121 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
      181 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
      241 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
      301 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
      361 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
      421 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
      481 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
      541 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
      601 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
      661 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
      721 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
      781 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
      841 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
      901 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
      961 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
     1021 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
     1081 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
     1141 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
     1201 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
     1261 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
     1321 actgcctcct
//
LOCUS       DAZAOG010000288         1298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000288 DAZAOG010000000
VERSION     DAZAOG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.298"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACHDLD_002889"
     CDS             117..656
                     /locus_tag="ACHDLD_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364535.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          485 a          171 c          207 g          435 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataat
//
LOCUS       DAZAOG010000289         1287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000289 DAZAOG010000000
VERSION     DAZAOG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.299"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACHDLD_002890"
     CDS             <1..848
                     /locus_tag="ACHDLD_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3364536.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAZAOG010000290         1245 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000290 DAZAOG010000000
VERSION     DAZAOG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.300"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHDLD_002891"
     CDS             200..742
                     /locus_tag="ACHDLD_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364537.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1245
                     /locus_tag="ACHDLD_002892"
     CDS             898..>1245
                     /locus_tag="ACHDLD_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN3364538.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          237 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgcca
//
LOCUS       DAZAOG010000291         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000291 DAZAOG010000000
VERSION     DAZAOG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.301"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHDLD_002893"
     CDS             414..947
                     /locus_tag="ACHDLD_002893"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN3364539.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZAOG010000292         1206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000292 DAZAOG010000000
VERSION     DAZAOG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.302"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..>1206)
                     /locus_tag="ACHDLD_002894"
     CDS             complement(56..>1206)
                     /locus_tag="ACHDLD_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364540.1"
                     /translation="EKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINI
                     RNVLFGVYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISL
                     LRAYGNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITE
                     EEFELDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMH
                     VNRKNWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLF
                     YDYLFLLVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRT
                     NIYLSDCLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          406 a          215 c          173 g          412 t
ORIGIN      
        1 aaaaaaacat aaatcagcta ataaatatgt gagacaaagc tgccatccat aaatactagc
       61 tgattttttc agttaaatgt ctattttttt ctctgatgat ggtatttcca aaatattccc
      121 agtcatctgg agtcggtgga tttttccaaa ttatctgttc tttattaaat ttttcaagta
      181 aacaatcaga taaataaata ttcgtacgag aagtgattcg actctcaatc acaatattgt
      241 aatttttaaa tcctttcact tgagaaataa tgtaatcggt ataagcagat ccttgcgaaa
      301 aatcaataca gacatgaatc ggtgcttcaa aatatgatac cggacaaata ccgaccaata
      361 aaaagagata atcataaaat aagcgaatca tgaaacgttc atcttttccc aagaaatagc
      421 gtttattctc ttgtatgacc ttttggattg ctgtactgaa tattggatac gtctcgaaga
      481 aaaactgtag ctgcttttta gacgtaaagc tagtcatctc aaagtcgaat atccaatttt
      541 ttcgattgac atgcatcata cccatacgaa gttcttttcg tatatatagt ctttcttctt
      601 cagagatttc aatcgaaaat ggtaattgat caatcaattc cactgacatt ttttgactaa
      661 tcatgtggat atcatgaaaa tgagcttccg caagtccaaa tgcgccatct aattcaaatt
      721 cttcttccgt gatcaaaaac aacatgatat aagaaatctc atcaatgatc tgctctttag
      781 ggatatgaag aaaagaaata tgtgataaat ccttgatgat cttcattcct tctgagctct
      841 taaagtattc atctgaaaag aaacgttttc cgattggttg attcccatag gccctcaaca
      901 aactaatagt ggttagcaaa ttgaatttga ttcgttttgt tgcagacagt tttaaattga
      961 acagatattc aaaatattgc acgatactct ttgcttcatt catgacgctt tctctaaatg
     1021 gcatagaaaa accattaaag accccgaaat ataccccaaa taatacgttc cgtatgttta
     1081 tttcttcccc aactaatatg tttttcttta actttatttg atgctgttca agaaaagaga
     1141 ctaattctct tctttttaca taggctcttg atatgcttaa aaaatgattc tctgcaaatt
     1201 tttcga
//
LOCUS       DAZAOG010000293         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000293 DAZAOG010000000
VERSION     DAZAOG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.303"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHDLD_002895"
     CDS             <1..1076
                     /locus_tag="ACHDLD_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN3364541.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZAOG010000294         1151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000294 DAZAOG010000000
VERSION     DAZAOG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.304"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACHDLD_002896"
     CDS             <1..153
                     /locus_tag="ACHDLD_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN3364542.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACHDLD_002897"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACHDLD_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACHDLD_002898"
     CDS             681..944
                     /locus_tag="ACHDLD_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3364543.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1151
                     /locus_tag="ACHDLD_002899"
     CDS             944..>1151
                     /locus_tag="ACHDLD_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3364544.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVY"
BASE COUNT          438 a          149 c          231 g          333 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat a
//
LOCUS       DAZAOG010000295         1135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000295 DAZAOG010000000
VERSION     DAZAOG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.305"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..264
                     /locus_tag="ACHDLD_002900"
     CDS             79..264
                     /locus_tag="ACHDLD_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364545.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            276..524
                     /locus_tag="ACHDLD_002901"
     CDS             276..524
                     /locus_tag="ACHDLD_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364546.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            555..734
                     /locus_tag="ACHDLD_002902"
     CDS             555..734
                     /locus_tag="ACHDLD_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364547.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(777..>1135)
                     /locus_tag="ACHDLD_002903"
     CDS             complement(777..>1135)
                     /locus_tag="ACHDLD_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364548.1"
                     /translation="YTKVIISNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGS
                     VDDNRIYSEAVPLNIPPKSAISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVD
                     IKNTNFPIEKLVKELN"
BASE COUNT          399 a          154 c          226 g          356 t
ORIGIN      
        1 aaaaatatta gttattgtca acgaatatta gctaattttt tagcttacag attatttttt
       61 tagaaaggag ctatttttat gtctgagaat ttagacttaa aaattcgagc ggagatgaga
      121 aaaagaagaa tgactttcaa agaactagct gcgcttatcg gtatttcagg agcttattta
      181 tcagatattc taaacggcaa tcgtgatgga aagaaagcac aacagcatat cgaaacagtg
      241 aaaaaaatat tggacatccg ataggggggt aggcgatgac aaaactaaaa aaacaagatt
      301 ttgtaaaaaa atacaattat tctccatcta cttatcaacg tcgaatgtcg gaactaaaaa
      361 atacagcaat tttctcagcg gcgtatgaac gggtcacagg acaagaagtt tggatcaata
      421 cagaattata cgataaattt ttgtctttca aatcctataa caggttacgc acaagaaagg
      481 taacacctaa agaatttatc gagaagcatt tagttgattt ataaaaaaca gaacattttg
      541 agaggtgaag gttaatgggt aaattcaaca gagcattggt attcagcgca ccgctaatca
      601 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
      661 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
      721 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
      781 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
      841 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
      901 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
      961 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
     1021 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
     1081 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaa
//
LOCUS       DAZAOG010000296         1114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000296 DAZAOG010000000
VERSION     DAZAOG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.306"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACHDLD_002904"
     CDS             <1..75
                     /locus_tag="ACHDLD_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3364549.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            80..1027
                     /locus_tag="ACHDLD_002905"
     CDS             80..1027
                     /locus_tag="ACHDLD_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN3364550.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          356 a          170 c          237 g          351 t
ORIGIN      
        1 gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat
       61 ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg
      121 ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc
      181 aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt
      241 cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca
      301 tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt
      361 gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa
      421 acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa
      481 agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca
      541 atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa
      601 tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt
      661 agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg
      721 aagccagtct agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc
      781 gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact
      841 tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa
      901 agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat
      961 tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat
     1021 caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt caataaggac
     1081 tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DAZAOG010000297         1110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000297 DAZAOG010000000
VERSION     DAZAOG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.307"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..821
                     /locus_tag="ACHDLD_002906"
     CDS             <1..821
                     /locus_tag="ACHDLD_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFN3364551.1"
                     /translation="YYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNKNVQVSILE
                     EGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLV
                     TAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDN
                     LIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMS
                     AKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            818..>1110
                     /locus_tag="ACHDLD_002907"
     CDS             818..>1110
                     /locus_tag="ACHDLD_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3364552.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSV"
BASE COUNT          425 a          133 c          187 g          365 t
ORIGIN      
        1 cctattattt gaaaaactgt aaaagtgaat attttgatga atttatttgt ttttcaatgg
       61 gagaggtttg gttggaagga atatatgctt gcttatatcg aaataataaa aatgtacagg
      121 tatcaatttt agaagaaggt attttaagct atggagttga cattattcca acagcaagaa
      181 caaaagctat cgcagtactc aattacttaa aaaaaaggac tccactatat aacaaaagct
      241 ataattttta ttgttttttt cctaatatat atacaggaag agagaataca atacaaattc
      301 cattagttac agctgggtca agaaccgcat tgcaactttc ggatattttt aaccctatga
      361 tagatattta taaagaaaaa aaatatatat tttttacgtc tgtttatgat tttgagggcg
      421 gagaacctgt cggagaatat gaattagtat gcaaaatagc agacttagta ggaaaagata
      481 atctaattgt taagacgcat cctagagatg taagaacaat ttataaagat aatggattta
      541 tcgaggataa aaattcatca ataccttggg aggcgattca attgtcaggc gattttaacg
      601 ataaaatatt tatgacaatt aattcaggaa gtgttatttc aggtagtaca atgtctgcaa
      661 aacctgtacg cacgtattat atgtataaat tgtgtaacat agaaggaaat ccgtcttgca
      721 aaaagaatgc gtatgatatt gagcgattgc ttaagcaaaa aacgatgaaa aacattttaa
      781 agaatgtgaa aatagcagaa agcttagatg atatactatg agcaaaacta aatatttaat
      841 taaaaatatg ggaatattaa caataagtaa tttttcttca aaaattttag tttttttgtt
      901 agtcccttta tatacaagtg tattatccac aacagaatat ggcacatatg atttgacaat
      961 ttcaacagcc acattattgt ttccaatttt aaccttaaac attgtagatg ctgtgatgcg
     1021 ctttttaatg gataaagata aaaataaaaa aaatatagca tctatagcga ttaaagttat
     1081 atttttaagc gtgatagtct ttggatccgt
//
LOCUS       DAZAOG010000298         1107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000298 DAZAOG010000000
VERSION     DAZAOG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.308"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHDLD_002908"
     CDS             <1..1087
                     /locus_tag="ACHDLD_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3364553.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          193 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaac
//
LOCUS       DAZAOG010000299         1084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000299 DAZAOG010000000
VERSION     DAZAOG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.310"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..738)
                     /locus_tag="ACHDLD_002909"
     CDS             complement(364..738)
                     /locus_tag="ACHDLD_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3364554.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(761..>1084)
                     /locus_tag="ACHDLD_002910"
     CDS             complement(761..>1084)
                     /locus_tag="ACHDLD_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFN3364555.1"
                     /translation="FVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQMFDKGLQRPLF
                     LEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDYSIN
                     YECGF"
BASE COUNT          365 a          208 c          131 g          380 t
ORIGIN      
        1 aataaaaaaa ggcatacaga caataaactg tctatacgcc tcacagactc tattctatac
       61 atctaatttt ggctgtcctg caaaaaatat gtgttagcct ccatcgaaag atcaaacttt
      121 ggaaaacatc attataactt tgcttggtaa gagctttgct aaaacatgat atgacttgta
      181 tacaaaatgt ggagtgtatt ccaataaata ggaaggatta caaaatttga aaccaaataa
      241 gtaaaaaata ctgtcacttt gaattaattt gaaatagtaa attctcatta aactattgaa
      301 atgctgatac caaaaaatga agtacttttc gttcatacca acttagagca cttcgttatc
      361 tatttatttc ttcatttggc ttatctctat aatctcatga tttggtccgt agaaattaaa
      421 gtaacggatt ccattttccc agaacggtaa ttccataata tttttatcta ataattcata
      481 tcctaaatct tgaattgaat tgaatacctc atctatgtca tccgtatcta atgctagatg
      541 atcaatggct cctctacact cagtagtttt atcttcccat atttctatta tcgttccatt
      601 caactcgaaa aagccgactt tacttcccac attcatttct tcattaatca gactaaaccc
      661 taaggattca taaaagattt ttgaagctcc aaaatctttt gttggaattc caatatgttg
      721 taaaccattt aatttcactt acatcatccc ttcaattgtt ttaaaatcca cattcataat
      781 ttattgaata gtctagttta tctaccttga ttgctccctc cgtaacagtc atacttaatg
      841 ctgttccgac tttatcatac attctagtac ttaaagcaac attatcaata tataaaacag
      901 ctatcgtatc atcaacaatt aactttagtt catattcttt gcctatttcc aaaaatagtg
      961 gtcgttgcag ccccttatca aacatttgag gccaaggata gttcggagtt tttccgaaag
     1021 taagctgatt attaatcaat gaaatacgaa attcgtatcc ttcttgagta tcactatcta
     1081 caaa
//
LOCUS       DAZAOG010000300         1071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000300 DAZAOG010000000
VERSION     DAZAOG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.312"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHDLD_002911"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHDLD_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN3364556.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          315 a          220 c          181 g          355 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa c
//
LOCUS       DAZAOG010000301         1069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000301 DAZAOG010000000
VERSION     DAZAOG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.313"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHDLD_002912"
     CDS             <1..969
                     /locus_tag="ACHDLD_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN3364557.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZAOG010000302         1068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000302 DAZAOG010000000
VERSION     DAZAOG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.314"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..846)
                     /locus_tag="ACHDLD_002913"
     CDS             complement(37..846)
                     /locus_tag="ACHDLD_002913"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3364558.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          323 a          202 c          188 g          355 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 cggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gatggatt
//
LOCUS       DAZAOG010000303         1031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000303 DAZAOG010000000
VERSION     DAZAOG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.315"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..887
                     /gene="galU"
                     /locus_tag="ACHDLD_002914"
     CDS             6..887
                     /gene="galU"
                     /locus_tag="ACHDLD_002914"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN3364559.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          368 a          172 c          238 g          253 t
ORIGIN      
        1 agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca cgttttttgc
       61 cggcaacaaa agccatggca aaagaaatgc ttccaatcgt ggataaacca acgatccagt
      121 ttattgtgga agaagcattg gattcgggaa tcgaagacat cttgatcgta acgggaaaag
      181 aaaaaagacc cattgaagat cactttgatg caaatatcga attggaaaac aatttgcggg
      241 aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac gactcaagtc aatcttcact
      301 tcatccgtca atctcatcca agaggactgg gggatgccgt gcttcaagca aaagcattta
      361 taggaaatga accgtttgtc gtcatgctgg gggatgattt gatgaaagat gatatcccat
      421 tgactcaaca attgatgatg gattatgaag aaacacaagc ttccgctgtt gccgtcatgc
      481 gtgtaccaga aaatgaaaca tctaaatacg gaattattga tcctaaaaca gatagcgaaa
      541 agggtcgttg cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa gcaccaagta
      601 atttagccat tatcggacgt tatctactga ctcctaaaat atttgagata ttagaaactc
      661 aagaaccagg agcaggaaac gagatacagc taacggatgc acttcagact cttaaccaga
      721 cagaggcggt ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc gataagttag
      781 gatatatgaa gacaaatatt gaatatggac ttcaacattc ggaaattggg acttcattaa
      841 gtgcatatat tatccagttg agtaaaaagc taagaacaga aagttaactg gaaataagtg
      901 gcctgctgag aatgaaaggc tatttgttat taaagcggca gcttgtgcgt catttgcgca
      961 actgtcgttt tagttttttg actctagatc agttgagtcg tggaaaacga aagatagaaa
     1021 aagactttcg t
//
LOCUS       DAZAOG010000304         1025 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000304 DAZAOG010000000
VERSION     DAZAOG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.316"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..>1025
                     /locus_tag="ACHDLD_002915"
     CDS             196..>1025
                     /locus_tag="ACHDLD_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3364560.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          361 a          149 c          175 g          340 t
ORIGIN      
        1 agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat ctttagtgat
       61 ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta aatagatatt
      121 tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac cgaaatttta
      181 taggaggact ttctcatgcc caattttact acagaaatta tggaaacact aatcaataaa
      241 ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc attattgcag
      301 gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt caattcaggt
      361 aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga attaaatttg
      421 gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc ttataaaaga
      481 accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat tactatgtct
      541 gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca aaccatttca
      601 aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc cttagaatcc
      661 agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca aactgtttca
      721 aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga ggttcttggg
      781 tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca agacctaaaa
      841 gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg cattaacgat
      901 agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca tatatctaga
      961 aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga tttcaaatta
     1021 gttta
//
LOCUS       DAZAOG010000305         1017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000305 DAZAOG010000000
VERSION     DAZAOG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.317"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..507
                     /locus_tag="ACHDLD_002916"
     CDS             97..507
                     /locus_tag="ACHDLD_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3364561.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            521..877
                     /locus_tag="ACHDLD_002917"
     CDS             521..877
                     /locus_tag="ACHDLD_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3364562.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          346 a          153 c          193 g          325 t
ORIGIN      
        1 gttattttga ttttttttta tatattatta gctaataatt ggtattttaa aagaaattac
       61 tgtagaattg gattataatt aaaaatgagt gagaaaatga taaaagtata tacttcgcct
      121 agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa
      181 gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg
      241 actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc
      301 atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca
      361 ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat
      421 gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta
      481 caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg
      541 atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc
      601 ttatcaattt gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg
      661 cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa
      721 cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca
      781 taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa
      841 tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc
      901 actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc
      961 tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt caaaatt
//
LOCUS       DAZAOG010000306         1013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000306 DAZAOG010000000
VERSION     DAZAOG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.318"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..>1013
                     /locus_tag="ACHDLD_002918"
     CDS             33..>1013
                     /locus_tag="ACHDLD_002918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFN3364563.1"
                     /translation="MNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIY
                     LTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDM
                     PLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVST
                     RMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLK
                     KILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIE
                     DMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTK"
BASE COUNT          375 a          129 c          168 g          341 t
ORIGIN      
        1 actattatta tcttcaaata acgtctatct ttttgaattc ttggatttat caaatccaat
       61 taacatcgga cgataaatta tattccttca tcaatgatat taatagttgg tgtagtcgag
      121 aagttcaatt agtcaatcgt tttattcaaa taggggatat ttatttaaca aaaattcaaa
      181 aaaatgtttt ggttatttta tttaagttaa tgtgtagtaa agaatttcag caaaaacgtt
      241 tagcactgat tttggctcat ggagatctaa cggcaacctg tatagcaagt actgctaata
      301 ggatcgttga gagatatata tacgacgcca ttgatatgcc actaatgact tcaagtagtg
      361 agattgcaga caaaattaat agatacttac aagagtgtac cggatatgaa gaaattattt
      421 tacttgttga tatgggttca ctggagaaaa tttatctaaa tttggaacta gacgctcaaa
      481 aaaaattcgg actactaaac aatgtttcaa caagaatggc tattgatgtt gcaaatcaga
      541 tattattaga tttaccgctt aaacagattc ttgaacaagt aaaagaaaat caaaatgtgg
      601 attatattta tgaatcatca aatattaaga aggatatgat tgtctgttct tgtgcaagcg
      661 gattaggaac ttcgtttaaa ttaaaaaaga tacttgaaga tagctttcca agggatactg
      721 atcttgtcat agaaacgtgt gactattact ctttagtgaa tgaaaactat ttatcggagc
      781 ttcgtaaaga gaacaatatt ctttttattg ttggaacact aaatccacat ctagaagatg
      841 tagaattctt ttcaattgaa gatatgattt taggaatgaa tataagtaaa ttctccaatc
      901 aattgaatag acactttaat gataatgaac tgaaagtttt gaacaataat attctgaaga
      961 attttagtct aacgaattta atggagcaat tgaccatatt aaatccgact aaa
//
LOCUS       DAZAOG010000307          996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000307 DAZAOG010000000
VERSION     DAZAOG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.319"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>996
                     /locus_tag="ACHDLD_002919"
     CDS             <1..>996
                     /locus_tag="ACHDLD_002919"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3364564.1"
                     /translation="ETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDI
                     PGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLF
                     DVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSM
                     DMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRS
                     SVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSE
                     SIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLA"
BASE COUNT          382 a          120 c          176 g          318 t
ORIGIN      
        1 agagacagaa gttgacaaaa ttgatattca aaataaaaag attagttgtg taaaaaaaga
       61 tggtacaaga ttctctcaag aatatgacaa agttatatta gctacaggtt caaagcaaat
      121 tatgctagat ataccgggga ataatttagc taatattttt tcgataaaga attttccaaa
      181 tgcaaaagag atttttaaag ctttggatga tagtactatt aaaaaagtag gaattattgg
      241 agcaggatat attggagttg aaattgctga agccataaga aaaagaggga aagaagtata
      301 tcttttcgat gttgcagata gagtgttgtc gacttattac gaccgtagtt tttcagataa
      361 agttgaagaa atactatcaa aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa
      421 atatttagga agaaaaaaga ttgaaaaaat agtcactaca agtggtaccc aatactctat
      481 ggatatggtc gtacaagcta taggatttct atctaatagt ccaattgggg aaaaggaact
      541 cttgagagat gttagtggag catatctaac taatgaatac cagcaaacaa atataaaaga
      601 tatatatgct attggggatt gtgctacaac tttttttacg tccattaata gaagtagtgt
      661 tgatttttct atttctaatg ctctaagaac agcttatatt gcaactcaac atattaacaa
      721 tcaagatttt acaccttctg gttcccaatt tacaaatggc ttttctatat ttaattacaa
      781 tttttacgca gcaggtttga atttaaaaac agcacgatta ttaagtgagt ctatagatta
      841 tatagaaata gaagatttca ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt
      901 aagaattagc tttgaaaaaa atacaaagag aatagtaggt gtacaacttt gtagtcaaga
      961 agatttatct ggactattat caatgttttc tcttgc
//
LOCUS       DAZAOG010000308          995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000308 DAZAOG010000000
VERSION     DAZAOG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.320"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACHDLD_002920"
     CDS             complement(26..913)
                     /locus_tag="ACHDLD_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3364565.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAZAOG010000309          994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000309 DAZAOG010000000
VERSION     DAZAOG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.321"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACHDLD_002921"
     rRNA            complement(<1..>994)
                     /locus_tag="ACHDLD_002921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZAOG010000310          987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000310 DAZAOG010000000
VERSION     DAZAOG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.322"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..865
                     /locus_tag="ACHDLD_002922"
     CDS             <1..865
                     /locus_tag="ACHDLD_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN3364566.1"
                     /translation="EVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHAT
                     EDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYS
                     KNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGE
                     GSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAA
                     KLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTT
                     EQMESIYSRDH"
BASE COUNT          381 a          111 c          189 g          306 t
ORIGIN      
        1 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
       61 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
      121 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
      181 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
      241 tgaaagtctg tttcaaaacc atccctttga gatagttcat aatggtattg atttagagct
      301 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
      361 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
      421 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
      481 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
      541 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
      601 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
      661 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
      721 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
      781 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
      841 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
      901 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
      961 cgattgaacg aaaatgcagt ttttatt
//
LOCUS       DAZAOG010000311          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000311 DAZAOG010000000
VERSION     DAZAOG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.323"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHDLD_002923"
     CDS             <1..704
                     /locus_tag="ACHDLD_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3364567.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAZAOG010000312          950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000312 DAZAOG010000000
VERSION     DAZAOG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.325"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>950
                     /locus_tag="ACHDLD_002924"
     CDS             <1..>950
                     /locus_tag="ACHDLD_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3364568.1"
                     /translation="LIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFF
                     IANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTAD
                     RYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTF
                     VMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILS
                     AKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDI
                     FIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILII"
BASE COUNT          306 a          114 c          179 g          351 t
ORIGIN      
        1 tctaatacaa tttgctaaag ggttagaaaa agttaaagct atgggcatag caggtattat
       61 cagcacaatt gtaacaataa ttgccaatat tttcttcttg cttattctga aatggggatt
      121 aattggtttt ttcattgcaa atattttatc acagattatt tcagctactt atctgtcagt
      181 attagtgaga atatgggaat atatcgattt tgaggctaaa gatcctcagc taaagagaga
      241 aatgctaaat tactcgattc cattaattgc aactgttgtt ggatggtgga ttaatagtac
      301 agctgatagg tatgtggttt cctttatagt aggtgtctcg gcgaatggct tattgtctgt
      361 ggcatataaa atacctcaaa taattaatac tttgcaggga atatttatac aggcctggca
      421 aatttctgca attaaagaat atggagataa tgagactgca aaattttatg gaaatacatt
      481 tgttatgatt aatttgttaa tgtgcataac ctgttcaagt cttatcatat taactagacc
      541 gttttcacat attctttatg cgaaagactt ttatgaagcg tggaaatatg ttccattttt
      601 attaatagct aatgttttaa attgtgcatc aggtctgtta ggacctatct tatcagcaaa
      661 aaaagattct aaatccatga tgtggtctgc aatcataggg gcattagtta atgtgattat
      721 gagtgtagtc cttgtttata agataggggt tcaaggtgcc acaatagcca ctgttatatg
      781 ttcatatgta atatatgttg ttcgtaaacg agctgtggga aaagatattt ttataaagca
      841 ttatcatatt gttgtggaat cttggggatt attgtttgtt caggcaatca tagaaatata
      901 ttttaaaaat tatgtaatag aaattataat tatgattatt ttaattatta
//
LOCUS       DAZAOG010000313          931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000313 DAZAOG010000000
VERSION     DAZAOG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.328"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACHDLD_002925"
     CDS             <1..>931
                     /locus_tag="ACHDLD_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3364569.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAZAOG010000314          928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000314 DAZAOG010000000
VERSION     DAZAOG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.329"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            306..928
                     /locus_tag="ACHDLD_002926"
                     /pseudo
     CDS             306..928
                     /locus_tag="ACHDLD_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          312 a          175 c          174 g          267 t
ORIGIN      
        1 cttcaattat ctaaatctcg aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa
       61 cgaattacta ttcatggtct tagacacaca catgctagct tgcttttagc aagctagtgc
      121 aaatattaaa gatgtgcaag agcgtttagg ccactcttct attcaaatca ttatggatct
      181 atatattcat attacagaca aaagaaaaga agaaaccgta gcgcaattcg caaaatatag
      241 gctctataat ttatgggact gatgaatcaa aattgattct tcagtcccat tgttatttta
      301 ggtatatgat tcgtacattg aaagagttga aaaactatct tccgaagccg tcaaaaagga
      361 ccctgccaaa agtattgatg gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa
      421 tgagctttat ttgtgcggat ggcgagactg gaaaactcat tgatattttg cctacacgaa
      481 aattgtctcg attaacacac tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt
      541 tagtgacaga tatgaacgcc gcctacttcc aactgacaaa acgtgtctta ccaaatgcga
      601 aagtcgtcat tgattgtttt catatcatca aacatttgaa tcaggcgttc aatgattttc
      661 gtgtacaaga aatgaaaaga ttacaccagt tagggcaacg acgggaagcc gagaaagtga
      721 aaaagaactg gcgctttctt ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa
      781 catggcgcag ttttcgtgca cctaaatacc cgctactcac tgaagcaatg atgattgatc
      841 gattgctggc attttcaacg cctttaaagg acgcctatac ttttttccat gagctgaccg
      901 aagcctttag agacaaggaa tcagactt
//
LOCUS       DAZAOG010000315          914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000315 DAZAOG010000000
VERSION     DAZAOG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.330"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..>914)
                     /gene="menA"
                     /locus_tag="ACHDLD_002927"
     CDS             complement(137..>914)
                     /gene="menA"
                     /locus_tag="ACHDLD_002927"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN3364570.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          325 a          189 c          159 g          241 t
ORIGIN      
        1 cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
       61 aatataagga gctattttta tttggctgtt cttattactt gatgcagggc agttttttca
      121 caacctcttc atgctgttaa atggcttgca taatgattgt tagtaatagc cccaatacat
      181 aactggcatt gaataagacc atatttttga tggaatagct gaaactgata ggctggggta
      241 acgtttgctt gaattgatcc aaatgcttct tcacaattgg caaggtcaag aaggtgacca
      301 agatcggcca ttcatagata ccgctcaaca accccattaa aatcacgaaa tagcaagcat
      361 acatcaatag ctggaataag actgttcccg caggacgtcc gatataaaaa actagtgtat
      421 agcgatgatt ttcaatatca cggtcaagat cccgcaaatt attagccaac atgatattgg
      481 caatcgtaaa caccaagggt aaggaagcta atacgattgc tgctatagcc caaagatttc
      541 ctaccaaatc aaattgtcca gatgaaaagt tgactgtcag atcatagaca tctgaaccga
      601 atgtattgac ataaacgacc atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa
      661 tttcccctaa aggcattcga gataaaggaa tcggtccgaa tgtatagaat actccaataa
      721 aacaacagat aaatcccatc aaaagtaaaa gccaacccgt attgatcgtt agaataacgc
      781 cgataacaaa aacaaatgca atcatagcta aaatcatgtt tcgaatcaat acaggagata
      841 gatttctgcg gccaatgaca ttttcttcat acttatatac ttcagaatga gccttttcat
      901 aatccatgta atta
//
LOCUS       DAZAOG010000316          908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000316 DAZAOG010000000
VERSION     DAZAOG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.331"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..777
                     /locus_tag="ACHDLD_002928"
     CDS             <1..777
                     /locus_tag="ACHDLD_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3364571.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          314 a          139 c          173 g          282 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagataaa gtaaaaatgc gaaattaagt agatgagagt gatgatgttc gaaatatcta
      841 ttatttttaa taagaataca taagctatct agtttgtaaa caatgagaga aaaaacatca
      901 atattttt
//
LOCUS       DAZAOG010000317          905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000317 DAZAOG010000000
VERSION     DAZAOG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.332"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..889
                     /locus_tag="ACHDLD_002929"
     CDS             266..889
                     /locus_tag="ACHDLD_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3364572.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          276 t
ORIGIN      
        1 ttagttttaa aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa
       61 tacttaaagg aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt
      121 atttgttgtt tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg
      181 atttttcagc ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa
      241 atagattgga taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga
      301 tcaaagttta gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc
      361 tgaagacgat ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc
      421 agagctgcaa gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct
      481 tgatcgatta gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa
      541 aggtgcttct attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt
      601 aagagacttg ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg
      661 gaaaaaaatc aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta
      721 tactggtaaa aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga
      781 tgaaattgtt gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg
      841 agttacaaga aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt
      901 tagaa
//
LOCUS       DAZAOG010000318          899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000318 DAZAOG010000000
VERSION     DAZAOG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.333"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>899
                     /gene="bsh"
                     /locus_tag="ACHDLD_002930"
     CDS             6..>899
                     /gene="bsh"
                     /locus_tag="ACHDLD_002930"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN3364573.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAID"
BASE COUNT          306 a          122 c          164 g          307 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgat
//
LOCUS       DAZAOG010000319          892 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000319 DAZAOG010000000
VERSION     DAZAOG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.334"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..871
                     /gene="thiD"
                     /locus_tag="ACHDLD_002931"
     CDS             56..871
                     /gene="thiD"
                     /locus_tag="ACHDLD_002931"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN3364574.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          320 a          145 c          204 g          223 t
ORIGIN      
        1 gaaacagtaa cagtattatg aaaaatcttt ttataaaaat ggaggaatcg atttgatgga
       61 agatcagtat gtacaagcaa tgacgatcgc aggatcagat agcgatggaa gcgcagggat
      121 gcaagcagat atgcatacat tctttacaag aaatgtttac ggtgtttctg tcatgaccgc
      181 atgtgttgca ggtaattctt acggaatcgg agcaagtgtg acattgccaa cagattttat
      241 cgacaaagaa tttgaactga ttgcgaagga ctttcaaata cgtgcagcta aaacaggtat
      301 gctagcagat tcaaagttga ttgagacagt tgtaaaaaat taccaaaaat atgattttgg
      361 accattagtc gtggatcccg taatcgtaac gaaacatgga aatcttttac tagaagaatc
      421 tgctttgcaa agcttgaaag aaaagcttgt ccctttggcc gaagtactga cacctaattt
      481 ttatgaagca gaaaagctga ctggacggac attagcagct gaagaagatt ttgtgcaagc
      541 agcaaaagaa ctgcaagata tgggtgcaaa aaacgttatg atcaaaggac atcatgctaa
      601 aaatgcgcag caagaaacag tcaatgacta cgtattgttc gaatcaggaa aatcattttg
      661 gttaaatgca ccattttatc ctaccgaccg gatcaacgga actggcgatt cgctatctgc
      721 atgtattgca gctgagttag cgaaaggaaa aacagttgaa caagcgatcc gattggcaaa
      781 agactatgta aaccgtgcaa ttcgtgaaga aataaatgta gggcacaaat atggccccat
      841 caatcattgg gcagctggtg aattagagta aaagaaagaa gttgtgaaaa ag
//
LOCUS       DAZAOG010000320          891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000320 DAZAOG010000000
VERSION     DAZAOG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.335"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>891
                     /locus_tag="ACHDLD_002932"
     CDS             <1..>891
                     /locus_tag="ACHDLD_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFN3364575.1"
                     /translation="IAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLT
                     KRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNR
                     ANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPD
                     LFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVS
                     YGFKSFENMRIRIFLINQLI"
BASE COUNT          300 a          175 c          168 g          248 t
ORIGIN      
        1 ttattgcgaa aaattgtcag gtatctttga ccaccgttat tcgtacgttg aaagagttta
       61 aaagctactt accaaagcaa tctaagagga ttctccccag agtattgatg gttgatgaat
      121 ttcgttcgca cgcttccata gaagataaaa tgagctttat ttgcgcagat ggcgaaacag
      181 gacaattaat tgatgtcttg cctacgcgta aattgcctcg attaacaagc tatttcttag
      241 gctgcgctaa tcctgaagaa gtaaaatttt tagtcacaga catgaacgcc gcctacttcc
      301 agcttaccaa acgtattctg ccaaatgcga aagtagtgat tgatcggttt catattatca
      361 aacacatgaa tcaagccttt aatgagttgc gtatccgtga aatgaatgaa cttcgtaaag
      421 taggacagaa aagtcaggca gaaaaactga aaaagaactg gcgctttttg ctaaaaaatc
      481 gtgcaaacat caaccattat gaatacaaaa catggaaaag ttttcgagca ccaaaatacc
      541 catttcttac ggaagcgatg atgattgatc gattgcttgg cttttctacg tccctgaagg
      601 aggcgtatcc ctattttcat gaattagttg aagcctttcg agacaaagac cctgacttat
      661 ttttctcctt attggcagaa cttcccgaaa cgttagatga cggctttcgg gaaaagctcc
      721 aaaaccttct gacctatgaa gaaggcatca ccaacgcgat gatctatcct tacaccaatg
      781 gaaaaataga agcgaagaat acccatataa agacaatgaa acgagtatcc tacggattta
      841 aatcatttga gaacatgaga attagaatct ttttgatcaa tcaattaatc a
//
LOCUS       DAZAOG010000321          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000321 DAZAOG010000000
VERSION     DAZAOG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.336"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHDLD_002933"
     CDS             complement(<1..244)
                     /locus_tag="ACHDLD_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364576.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQF"
     gene            complement(246..>890)
                     /locus_tag="ACHDLD_002934"
     CDS             complement(246..>890)
                     /locus_tag="ACHDLD_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN3364577.1"
                     /translation="KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNES
                     DKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFL
                     KKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSK
                     VNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          335 a          154 c          105 g          296 t
ORIGIN      
        1 aaaattgatt atggttagtt aacaagttaa ttttacgttt aaaggaagaa ccgattttcc
       61 ggtgattcac aaaaacaaca tcattaaata gccctaattc ttttatacgc tcactatatc
      121 ttctagcgtt ttcaaattca gaataaataa ataaatctcc ttttgattct cggtaaaaat
      181 tttgataaac atttaatata ttaatcaaat gtaaaggagt ccaagcaatg aaagctgtct
      241 tcatattaat tctccttttt taaatacaag taagaaatgc ttcccaaata ataccagaaa
      301 acaattgaaa tgacatcgcg cttattgaaa agtacatgag cctccaccat ttcatctata
      361 aaaatagata gtataaaaat taaaacaata ccaagaaacg cataattcac tttacttcta
      421 aaaaatgttt tgagtaaact aaagatattt aacacataga aagcatagat acatataaaa
      481 gcaattatac cacttgatac aagtacttga agaaatccat tatgcatgtt tccatgtgct
      541 ctttttagtt cattcacatc tgtagtatca agatctactt ttttcaaaaa ttttgcatcg
      601 ctattcccat agaaatcggt ctctccataa ccaaataaca aagttggtct tataacttta
      661 agcgcagctt tccaaatcgt aaaacgacca gctgaaattt cggagttatc actagaatgc
      721 tgtaatatta cttctttttc tggaagtgga gttttatctg attcatttga ctctataact
      781 tctacttttc tatcaatcga tacaggaatt gttgctaaaa aatccttagc ataattagta
      841 ccaaaagaaa aatataccaa tagaactaat aatatgctta ccatccattt
//
LOCUS       DAZAOG010000322          876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000322 DAZAOG010000000
VERSION     DAZAOG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.337"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACHDLD_002935"
     CDS             <1..75
                     /locus_tag="ACHDLD_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN3364578.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            62..196
                     /locus_tag="ACHDLD_002936"
     CDS             62..196
                     /locus_tag="ACHDLD_002936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364579.1"
                     /translation="MTMNRAEALRIGTAIANCWWKYYKPIILSQQHIDKQKAWQQIKK
                     "
     gene            253..594
                     /locus_tag="ACHDLD_002937"
     CDS             253..594
                     /locus_tag="ACHDLD_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364580.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            587..>876
                     /locus_tag="ACHDLD_002938"
     CDS             587..>876
                     /locus_tag="ACHDLD_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN3364581.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          325 a          142 c          185 g          224 t
ORIGIN      
        1 ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg
      121 gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca
      181 acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg
      241 agattttagc atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat
      301 tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg
      361 aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat
      421 gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa
      481 gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat
      541 tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa
      601 agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa
      661 aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc
      721 acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca
      781 agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc
      841 tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DAZAOG010000323          872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000323 DAZAOG010000000
VERSION     DAZAOG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.338"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACHDLD_002939"
     CDS             <1..75
                     /locus_tag="ACHDLD_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN3364582.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..590
                     /locus_tag="ACHDLD_002940"
     CDS             249..590
                     /locus_tag="ACHDLD_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364583.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            583..>872
                     /locus_tag="ACHDLD_002941"
     CDS             583..>872
                     /locus_tag="ACHDLD_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN3364584.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          335 a          138 c          176 g          223 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
      541 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg
      601 acaacgaact ttgaaaaact ttttagtcgt aagttaaata aaattctcaa gaaaaaagga
      661 aattttgatt atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag
      721 gcaacggtaa ctattaatga gtataaacac tacagggttg tttctggaac tcatcaagac
      781 tttcttgttg aggaatataa accttttctt atggatgaaa cagggactta tgtatctgtc
      841 tcagtaacgg ttaaaggaca tacggaaaca ga
//
LOCUS       DAZAOG010000324          869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000324 DAZAOG010000000
VERSION     DAZAOG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.339"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHDLD_002942"
     CDS             136..309
                     /locus_tag="ACHDLD_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364585.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          290 a          143 c          105 g          331 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttat
//
LOCUS       DAZAOG010000325          864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000325 DAZAOG010000000
VERSION     DAZAOG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.340"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..339
                     /locus_tag="ACHDLD_002943"
     CDS             43..339
                     /locus_tag="ACHDLD_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN3364586.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          321 a          132 c          160 g          251 t
ORIGIN      
        1 gagtaaactt atcaaataaa agacttcttt ggaggaatca ctatgaagga atatataaaa
       61 aacatctatt ttatcgaaga aacacaaaat atcgagggtt cttatattga agtaaaaaca
      121 ctctttgtca atgaagataa aaccaaagca ttggatattt ataaaaaatt agcttcaaaa
      181 aaaacgaata gttttggatt gatattaagc gagtataaaa tcaaagcaga agagagttat
      241 ttctatcaat tattaaaacg gtggagtaag cttcccgccg atttttatcg aaaaatgcag
      301 atcatcaact atcaaccgct tgctgagaca catgcctaaa tagaggttgt gaaatcagtg
      361 ctttgagcta ataccgaagg tcagctgatt gaacattgtt tagaagagat tgtgacaact
      421 gcgatttgct ttgagtaata agaacgctgg acagaaaata gcttctcata ttttctagtc
      481 tagtgggctt attgccgaga agcagcagat ggaacatcgt ttagaagaga ttgtgacaac
      541 tgcgatttgc tttgagtaat aagaacgctg gacagaaaat agcttctcat attttctagt
      601 ctagtggact tattgccgag aagcagcaga tggaacatcg tttaaagaag agatcggtac
      661 aaaataaaaa tatctaaatt taagttttat ctaagtttcg ctttgtacct ttgattcaaa
      721 tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac tattttttgc cactccccat
      781 tctagaatca acccgtatga tgtagataat gaattcacgt tgaaaaaaga tttctcaaat
      841 aaataaaaac catccgtaaa atca
//
LOCUS       DAZAOG010000326          848 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000326 DAZAOG010000000
VERSION     DAZAOG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.342"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>848
                     /locus_tag="ACHDLD_002944"
     CDS             14..>848
                     /locus_tag="ACHDLD_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364587.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     EL"
BASE COUNT          276 a          111 c          124 g          337 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattac
//
LOCUS       DAZAOG010000327          845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000327 DAZAOG010000000
VERSION     DAZAOG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.343"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..724
                     /locus_tag="ACHDLD_002945"
     CDS             <1..724
                     /locus_tag="ACHDLD_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN3364588.1"
                     /translation="EQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFN
                     LSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVS
                     LPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVS
                     ETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFK
                     KYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          307 a          100 c          150 g          288 t
ORIGIN      
        1 cgagcaaatc aaaatagata atctgttatc acaaaattac gatccagaaa tatatctgtt
       61 tctttatgaa aataaaatac tggaatatgt taaaaatgga gatattcgaa atttagaaaa
      121 catggttttt aatctgagta atggaataat tccaagtgtt agtggagata cgattcggtc
      181 tgaaaaaaat tattctatca ttgtttttga aaagttagca caaacgtcta taacattagg
      241 aatggatata atagaagcgt atcaaagtag agatgctttg atacaagaaa acgaattagc
      301 tgtatcgtta cctgaggtct taaaagtgag ggattctggg attgtttatt atactaagga
      361 aatcggaaaa actaagatag agcatctttc tcccttaatt tcttctgtcg ttcaattcat
      421 cggcttaaat atctataaaa gaattactgt aaaggaaata gctaattatt tttctgtaag
      481 tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat attacgattt ataattatat
      541 ctctaagcga aaaatttcta cggctaaaat aatgttgaaa tcaaaccata ctatcagtga
      601 agtttcttta ggtttaggat tttcagattc ttcacacttt tcgagagtat tcaaaaagta
      661 cgcaggtgta tctcctaagc aatatcaatt aggtttagtg gacaatttca acaacattag
      721 ctgagaggtt ttattttatt atgagactat atcatttacg tagtttggag gagttgagga
      781 aattggattt taacaaaaaa tttttttggg aaatttctga tttgagttgt ttcgtagaat
      841 ctgag
//
LOCUS       DAZAOG010000328          841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000328 DAZAOG010000000
VERSION     DAZAOG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.344"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..464)
                     /locus_tag="ACHDLD_002946"
     CDS             complement(243..464)
                     /locus_tag="ACHDLD_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364589.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..>841)
                     /locus_tag="ACHDLD_002947"
     CDS             complement(562..>841)
                     /locus_tag="ACHDLD_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364590.1"
                     /translation="RLLLLTAEAGWMDFPEVVKEVQEIGATISDSRSSPMNYQDAHGI
                     KIIDQERNVRKYTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          253 a          162 c          111 g          315 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 a
//
LOCUS       DAZAOG010000329          827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000329 DAZAOG010000000
VERSION     DAZAOG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.345"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..418
                     /locus_tag="ACHDLD_002948"
     CDS             182..418
                     /locus_tag="ACHDLD_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFN3364591.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            437..652
                     /locus_tag="ACHDLD_002949"
     CDS             437..652
                     /locus_tag="ACHDLD_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFN3364592.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            621..>827
                     /locus_tag="ACHDLD_002950"
     CDS             621..>827
                     /locus_tag="ACHDLD_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364593.1"
                     /translation="MLDSACGPVRKQLKNIKPQDWFVSITNIGMFFIFSKVWNGQNFL
                     LKLLVLVFIIILNFYLLLKSDKNSL"
BASE COUNT          268 a          111 c          187 g          261 t
ORIGIN      
        1 ctcataattc cacaaaaact ttgaaaacag ttcttgaaca ctaacttttt cattcctgaa
       61 tcaaatttat tttggaaatt ttactattca agaataaact tgataagttt caggaagatt
      121 tttactgtta atcaaatacg tatgttacag tagcaccgta aaaaatatta tttaaagaag
      181 cgtgtttaaa catgcaaaat gtaaaggaac taagtattgt ggaaatgcac aaactgttgg
      241 tggaggcaat aatgcttggg gaaaattggg gcaagttgta ggtggactta ccacaggggc
      301 agtaggtggt gcaggtttag gaacagcaat ttgcggacca gcttgcggtg ttgttggagg
      361 gttatatgga gctgtcgctg gaggtgctgc agctggctgg gatgctcgaa aaaaataaaa
      421 aaggagaata agtagtatgc atattaaaaa cacaaaaaca acatttatat tatcttcaga
      481 agagttgaaa aatattcaag gtgggagtgc cgtgggagtt ttaggcacta ctttttctgg
      541 ggcaacagca ggagtaaaac tttgttcagc cggaggacct tatgcaatag ctgcttgtgg
      601 tgtaggtgga gctttattag gtgctggatt cagcatgtgg accggtgcgt aaacaattaa
      661 aaaatatcaa acctcaagat tggtttgtgt cgattacgaa cataggtatg ttctttatct
      721 tttcaaaagt ttggaatgga cagaactttt tactaaagct tcttgtgcta gtttttataa
      781 ttatattaaa tttttatctt ttattaaagt cagataaaaa ttctttg
//
LOCUS       DAZAOG010000330          795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000330 DAZAOG010000000
VERSION     DAZAOG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.346"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>795
                     /locus_tag="ACHDLD_002951"
     CDS             <1..>795
                     /locus_tag="ACHDLD_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN3364594.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          270 a          117 c          148 g          260 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttca
//
LOCUS       DAZAOG010000331          785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000331 DAZAOG010000000
VERSION     DAZAOG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.347"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..127)
                     /locus_tag="ACHDLD_002952"
     tRNA            complement(55..127)
                     /locus_tag="ACHDLD_002952"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:His,seq:gtg)
     gene            complement(130..203)
                     /locus_tag="ACHDLD_002953"
     tRNA            complement(130..203)
                     /locus_tag="ACHDLD_002953"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Trp,seq:cca)
     gene            complement(210..290)
                     /locus_tag="ACHDLD_002954"
     tRNA            complement(210..290)
                     /locus_tag="ACHDLD_002954"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Tyr,seq:gta)
     gene            complement(292..367)
                     /locus_tag="ACHDLD_002955"
     tRNA            complement(292..367)
                     /locus_tag="ACHDLD_002955"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Phe,seq:gaa)
     gene            complement(403..475)
                     /locus_tag="ACHDLD_002956"
     tRNA            complement(403..475)
                     /locus_tag="ACHDLD_002956"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Asp,seq:gtc)
     gene            complement(485..557)
                     /locus_tag="ACHDLD_002957"
     tRNA            complement(485..557)
                     /locus_tag="ACHDLD_002957"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Val,seq:tac)
     gene            complement(573..644)
                     /locus_tag="ACHDLD_002958"
     tRNA            complement(573..644)
                     /locus_tag="ACHDLD_002958"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Glu,seq:ttc)
     gene            complement(659..748)
                     /locus_tag="ACHDLD_002959"
     tRNA            complement(659..748)
                     /locus_tag="ACHDLD_002959"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Ser,seq:gga)
BASE COUNT          215 a          215 c          183 g          172 t
ORIGIN      
        1 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
       61 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
      121 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
      181 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
      241 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
      301 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
      361 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
      421 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
      481 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
      541 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
      601 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
      661 gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat
      721 gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa
      781 atgag
//
LOCUS       DAZAOG010000332          779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000332 DAZAOG010000000
VERSION     DAZAOG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.348"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /locus_tag="ACHDLD_002960"
     CDS             <1..225
                     /locus_tag="ACHDLD_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3364595.1"
                     /translation="TRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKEL
                     KYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            226..>779
                     /locus_tag="ACHDLD_002961"
     CDS             226..>779
                     /locus_tag="ACHDLD_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3364596.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNAS"
BASE COUNT          304 a           92 c          138 g          245 t
ORIGIN      
        1 acaagaaaat ttcaattgtt tactttagat gaatgtatca atgttgactt attaaataat
       61 aaaataagtt ttgaagaaaa cattgttaaa gaaatgcctg aatctgctaa tgatatgtat
      121 gagaaggagt tgaaatattt ctttgaaaac gtagtaacag gcaaagaaaa ctggaatgat
      181 ttatatcatg caaacgaagt attgaaaata gctgaggaga aataaatgga aattttattt
      241 actatttgtg gtagagctgg gtctaagggg tttaaaaata aaaatttgaa atatttggca
      301 ggaaagcccc tagtccattt cgctttagca atgatagata tctatcaaca aaaacatcct
      361 gaacataatt gcacgacttg tttgaatacg gacagtaatg agttagtaga attgacgact
      421 aagttaaatc cgtctgtgaa aattgtagaa cgtaaacctg aattagctac agatactgta
      481 gggaaaattg aagtgatacg tgattcattc ttacaactag aaagagaaga taatatattt
      541 gatttaatta tagatttaga tttgacttca cctttacgtg aagtttctaa tcttgaagaa
      601 ttaatagatg aacatctaaa aaatttagat aaagacgtct acttttcagt tgtttctgca
      661 agacgtaatc cctattttaa tatggtaaaa agaattgaaa atgaagttac tatagttaat
      721 aaatcaagat acacatctag gcaacagaca cctgaagttt ttgaaatgaa tgcctcaat
//
LOCUS       DAZAOG010000333          766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000333 DAZAOG010000000
VERSION     DAZAOG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.349"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>766
                     /locus_tag="ACHDLD_002962"
     CDS             <1..>766
                     /locus_tag="ACHDLD_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3364597.1"
                     /translation="YDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNI
                     LFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHK
                     FKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAE
                     STDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLS
                     LILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIF"
BASE COUNT          318 a           64 c          133 g          251 t
ORIGIN      
        1 tatgatggat tagcaaaact aaaagatgct aaagtagtta gcggagaaca aagaataaat
       61 agagaagatt tatctgacga atttaagaat gttgtttcac tcgaagctac aaataatact
      121 aaaagaaata ttttatttag tagtggagta tttacaatta aagaaggtaa aaatatagaa
      181 gaaaatgata agaattcaat tattgttcat gaagaatttg ctaaacaaaa caatctaaaa
      241 ttaggtgatg aagttgatct tgaattacta gatattgaaa aaagtggaaa aataaaaagt
      301 cataaattta aaattatagg gatcttttct ggtaaaaaac aggaaacata tacaggatta
      361 tcttctgatt ttagtgaaaa tatggtgttt gtagattatt caacaagcca agagatatta
      421 aataattcag aaaataatga aattgcaaat aaaattttaa tgtattctgg tagtgcagaa
      481 tctacagact tagccttaaa caaattgaaa gagcttaaaa ttgatgagtc aaagtatttt
      541 gttgaaaaag attctaatgc gtttgaagag tctttagagt cagtgagtgg aataaaatat
      601 ataataaaag taatgactta ttctattatg ttaggtggga tggttgttct ttcattaatc
      661 ttgattctat ggttaaggga aagaatttat gaaataggta tattcttatc tattggaaca
      721 tctaagatac aaattataat gcaatttata ttcgagttaa tattca
//
LOCUS       DAZAOG010000334          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000334 DAZAOG010000000
VERSION     DAZAOG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.350"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHDLD_002963"
     rRNA            <1..>733
                     /locus_tag="ACHDLD_002963"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZAOG010000335          728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000335 DAZAOG010000000
VERSION     DAZAOG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.351"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>728)
                     /locus_tag="ACHDLD_002964"
     CDS             complement(39..>728)
                     /locus_tag="ACHDLD_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364598.1"
                     /translation="DKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRR
                     ELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFF
                     LFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKP
                     EFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQID
                     DFPEEIIDRIE"
BASE COUNT          225 a          129 c          109 g          265 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatc
//
LOCUS       DAZAOG010000336          726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000336 DAZAOG010000000
VERSION     DAZAOG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.352"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>726)
                     /locus_tag="ACHDLD_002965"
     CDS             complement(109..>726)
                     /locus_tag="ACHDLD_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFN3364599.1"
                     /translation="YTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRV
                     GKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFD
                     NQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFD
                     YVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          205 a          166 c          135 g          220 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtata
//
LOCUS       DAZAOG010000337          719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000337 DAZAOG010000000
VERSION     DAZAOG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.353"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..675)
                     /locus_tag="ACHDLD_002966"
     tRNA            complement(602..675)
                     /locus_tag="ACHDLD_002966"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
BASE COUNT          252 a          124 c          121 g          222 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatg
//
LOCUS       DAZAOG010000338          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000338 DAZAOG010000000
VERSION     DAZAOG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.354"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            420..>714
                     /locus_tag="ACHDLD_002967"
     CDS             420..>714
                     /locus_tag="ACHDLD_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3364600.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTD"
BASE COUNT          268 a          109 c           99 g          238 t
ORIGIN      
        1 ctaaatattc caatttggga atccaataaa aattttcgtt caaatacgtg tctattcgaa
       61 ctcttttcta gttgttaatt atgaaggggg aagaaataaa aaatacaatc tatacacgaa
      121 taagaatatt atccaaaagg aggttttaga aaagatttat tatacgtaaa aagctcaaga
      181 cattttctta gccataagga catgtttgat atttaagaag atatatgaat tttcttttct
      241 aagtataaag ataactatcc attatcaact aaaaaaacat ttttgaaaat aaacatcagc
      301 agtcctatca atgcatgtga gcttatagaa gttattcgca gcgcagcata attgcattag
      361 cttcttttcg tgttttttgc ctcttttccc tttaaatctt aatggaggag gtgtgcaata
      421 tgtcagataa cgacgaacgc gtaaatgccc agttgattcg attctttgaa aaggtcattt
      481 tgaacaccgc taatacttat tataaaaaac aagaagaaat tcaaaaacat gaacaacttg
      541 atcaactccc accatatttg tttactaaag atactataaa atttaaacaa ccgattaatg
      601 ttttaaatgt gactctcctt gttgaagata taaaattagc aaaaatcctt cctttgttga
      661 aagaacctga acaaattttt cttatcgaaa aatttatctt tgataaaact gata
//
LOCUS       DAZAOG010000339          699 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000339 DAZAOG010000000
VERSION     DAZAOG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.355"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..592
                     /locus_tag="ACHDLD_002968"
     CDS             <1..592
                     /locus_tag="ACHDLD_002968"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3364601.1"
                     /translation="ELLRIISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIG
                     IWGQLGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLN
                     SFVKEILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRM
                     LFKTSLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT          222 a           81 c          116 g          280 t
ORIGIN      
        1 tgaactttta agaatcattt ctatattgtg tattgttgct taccatgcta atttgcgttt
       61 aaattataat gcagacggaa ttatagtaca tggttcatta actgctaacc agattttctt
      121 gaattatatt ggtatttggg gtcagctagg agttaattgt tttatattta tttcggcata
      181 ttttttaagt gagaaaagaa acattcactt tgaaaatgtt ttgggaattg tattagaatg
      241 tattttttat agtgtattag tttatggaat tctttgtgca tgtggaatag aaacattttc
      301 attgaacagc tttgtaaaag aaatattatc atttgctcta ggtagatatt ggtttattac
      361 atcctattta gcattgtata ttctaagtcc gtatttaaat atcatgctta acaatctgga
      421 aaaacttcag ttgcaaaaaa taagtatgtt attgtttata ggtgtatgct gttatcgtat
      481 gttatttaaa acatctttgt ttggggattg tgctgtttta attgcactat atgtcatatg
      541 ttatacactg aaaaaaacga taataatatt tttgagagac acagaatcat aggaacaatt
      601 gtatgtataa cagttatacc acttctcata tccataggaa tacttttcaa atttatacca
      661 aatgcaatgg ttaaccagat ggttcatatt ggttctcca
//
LOCUS       DAZAOG010000340          698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000340 DAZAOG010000000
VERSION     DAZAOG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.356"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..446
                     /locus_tag="ACHDLD_002969"
     CDS             78..446
                     /locus_tag="ACHDLD_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN3364602.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          251 a          114 c          106 g          227 t
ORIGIN      
        1 tgttttgttc gttttttata cataactatt gtttttctgg taaaataaat caaaccactt
       61 aatggaggcg ttttagaatg gaaaaatttc agccgatcat caatcaaatc aaagaaaaag
      121 aacatcaaca aaagatcaaa gacctttttg ctaagataac agaaacgtat ccccagctag
      181 acacaactgt aaaatggaac caacctatgt ttactgacca tggtaccttt attattgcat
      241 ttagttttgc taaaaatcat ttttctatcg caccggaaaa agcagcgatt cgtgcccttg
      301 aaaaaaatat ccaagaagca ggctacgtat atacggataa cgtcatcaaa gttccttgga
      361 aaagtgtaat caactgggaa ttgattgaac aattgatttc atttaatatt gaagataaaa
      421 aaggtcatga taaattttgg cgataaagct ttatacgaat aaacctttta ctggcgagga
      481 aactattcat caccttatac aggttttgtt caatcctatc ctttttatca tccctggtta
      541 aacacttaat tgacaaagta tctgctattt ttataaggag tctttcttgt gctgaaaaaa
      601 atcactgatt gaatggcttt gaattttcta catagtaaaa ttattttttc agcaattttc
      661 ctgcaaaact tttgaataag gaaaaaatac gagaaaat
//
LOCUS       DAZAOG010000341          693 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000341 DAZAOG010000000
VERSION     DAZAOG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.357"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..612
                     /locus_tag="ACHDLD_002970"
     CDS             349..612
                     /locus_tag="ACHDLD_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364603.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT          281 a           84 c          114 g          214 t
ORIGIN      
        1 aatgatgaaa gtcagattga gtttttaaat gataccttcc tcagaacttc aaaacgaatt
       61 caaagattcg ttgacaaatc ttgagcaaga tcatttaaaa aaatattttc tcttcatttg
      121 acgaaaaatg tttagaaatc ttatataagt gaagataaag taattggtgt aattaattgt
      181 agagaatagc ctttgagcaa ttgaagcgat acaattattt agaaaatgaa cttcctccag
      241 catactacat gattccttac gggacgatgt taaagaagaa agcaagaaat tttagaagta
      301 ttagtagata aactaatggg cttttctcaa tttaagggag tagcccaaat gtttatcaaa
      361 tatggtgcag atacagtagc aaccatgttt acagaagaat atcaagtaga ctattatgaa
      421 gtaatagata aatgtgtaca ttttgtttat gaaaaagtgt ggaattttat gaattttgcg
      481 ataagtgaag aaatcttacg agtacaagaa aacagaacag gaatatttac cagagacaga
      541 aagcttaatt tgattgcgca ttatatacgg aatcgctggt catcaatacc aaattttaat
      601 tataaaaaat aattatgaac aatcttttat acaatataca caataaatca agtagtattt
      661 atgaaaaaag acttcaaaaa cagaatgatt ttc
//
LOCUS       DAZAOG010000342          686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000342 DAZAOG010000000
VERSION     DAZAOG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.358"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..448)
                     /locus_tag="ACHDLD_002971"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="ACHDLD_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT          206 a          136 c          105 g          239 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaa
//
LOCUS       DAZAOG010000343          669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000343 DAZAOG010000000
VERSION     DAZAOG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.359"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..463)
                     /locus_tag="ACHDLD_002972"
     CDS             complement(<1..463)
                     /locus_tag="ACHDLD_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3364604.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFST"
BASE COUNT          235 a          106 c           97 g          231 t
ORIGIN      
        1 tagtagaaaa cattatttta gtgagggaac agtctacaaa atctgcgctt gataagacag
       61 agtgctcaaa ggtactttct tttatgtttg aaccagataa atttacatag tccattttac
      121 aattctcgaa taacgtgttc ccaatataac tttccatcaa tttagtacct gttaatttac
      181 aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa tatacagcct gaaaaatcac
      241 agttttcgaa tatacagtcg gtaatctcca ttctttgaaa atcattggaa gcaaaaaatg
      301 catacttgaa aatagatttg taaataattg gatggcagat tatttggttt tcgatgacct
      361 ctttttgtga aaggatatta tacagttcaa aatcttcgtt ttcatagata tgaaaaaagg
      421 atcctttatt caagcttgct tcattaactt taggaaaaat catatttaat tacctccatt
      481 ttcataaagt catattaaca aaagctaaac caacttacat tagcagaaaa tgagataggt
      541 tttcgccttt ttcgataacg atagtgtcgc tatctcttga aacgttctta gaattgccct
      601 gagagacaca aattaggcgt aattctaata aaaatcttag aataaaaatt tttactgatt
      661 taatttaga
//
LOCUS       DAZAOG010000344          662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000344 DAZAOG010000000
VERSION     DAZAOG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.360"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..>662
                     /locus_tag="ACHDLD_002973"
     CDS             46..>662
                     /locus_tag="ACHDLD_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3364605.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLD"
BASE COUNT          255 a          101 c          115 g          191 t
ORIGIN      
        1 atatcaaatt caaaaatgaa attttgagag ggattaaata tgacaattaa aactagtctc
       61 caaaatcaag cttatcaatc tattcgtcag caaattatct acgctgactt agaacctgga
      121 aaaaaagtat cagaaaaaaa attggaagaa acgctttcta tcggtcgtac tccaatacgt
      181 gaagcattga ttcaactccg tcatcaagga cttgttgata caattcctca aagtggaaat
      241 tatatctcat taatcgactt agatttagca aaaaatgctc gttttgtcag agaacattta
      301 gaacgacaaa ttatgctaga gtgttgtgcc aagatgaata atcaaaagaa acaagtttta
      361 gaaactataa ttgcagaaca agaaaaagct gttagtcaaa agaatgctcg tagttttttt
      421 cataatgata atttatttca taaagcttgt tttgaaattg cagaacggga agaagtatgg
      481 caatggttgg aagataataa tacgcacttg gaacgatttc gttggttacg agtaaaaacg
      541 gaaggattaa aatgggaaaa aattatggaa caacatcatc aactatttaa agccttgacc
      601 atgcaagacc ctgaagaagc taacttctta acatccttgc atcttcattt aatgcttgat
      661 ga
//
LOCUS       DAZAOG010000345          646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000345 DAZAOG010000000
VERSION     DAZAOG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.361"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..619
                     /gene="spxA"
                     /locus_tag="ACHDLD_002974"
     CDS             221..619
                     /gene="spxA"
                     /locus_tag="ACHDLD_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN3364606.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          230 a           96 c          122 g          198 t
ORIGIN      
        1 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
       61 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
      121 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
      181 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
      241 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
      301 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
      361 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
      421 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
      481 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
      541 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
      601 attgatggct ggattataga aggataatac gaaacttgga aaagac
//
LOCUS       DAZAOG010000346          642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000346 DAZAOG010000000
VERSION     DAZAOG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.362"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..568)
                     /locus_tag="ACHDLD_002975"
     CDS             complement(98..568)
                     /locus_tag="ACHDLD_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN3364607.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
BASE COUNT          217 a          135 c           95 g          195 t
ORIGIN      
        1 tcttgaaaaa tctacggcta atgcaatagc attcggaaca ggaatcaact tgagtacagt
       61 aaaaaaactt aaaagtggag agagagctga agaaaaatta aatcttgccg atgctattaa
      121 aatcacagaa tttggcatga aaaatatgcc aaccaaaatc gaaatatgga aatcctagct
      181 cttccaaagc catttgtcgt tggtaccaaa cactgccgag acttttccca ataatatcgc
      241 caatttcacg atcattaaga ccgatgaaga aggacataaa aatgatttct tgtctttgac
      301 taggtaatgc tgcaatcgca tttgccaact cctcattgga aataaagatt ttcataccaa
      361 ggacaataaa agtaccacta ctattcaaat ttggttccaa ataatagaac tctgtaccta
      421 accctttttg ttccaatatt tccattgata tttgccgttg gcgaaatcta gcgtacttct
      481 tttgaatatt taacgcctca ttgcagacta ctttcttaca aaaactgtca aacgtatgtt
      541 gaatgtgggt ttcctctgag tcgcccataa tattctcccc ctcaaaaaat tcattcaact
      601 cgttttactt ctatcccttt ccattattac tacaatctag ga
//
LOCUS       DAZAOG010000347          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000347 DAZAOG010000000
VERSION     DAZAOG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.363"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACHDLD_002976"
     CDS             complement(<1..270)
                     /locus_tag="ACHDLD_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364608.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACHDLD_002977"
     CDS             complement(276..>635)
                     /locus_tag="ACHDLD_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN3364609.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAZAOG010000348          595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000348 DAZAOG010000000
VERSION     DAZAOG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.364"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..504)
                     /locus_tag="ACHDLD_002978"
     CDS             complement(205..504)
                     /locus_tag="ACHDLD_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3364610.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          192 a          106 c           69 g          228 t
ORIGIN      
        1 tagtttcata aaatacactt ttaattaaca ttattagttt atactagctg tttttgtgtt
       61 aattaaagga taaaatacaa tataaagaaa catctgtttc tttatttaga ataaaaaaat
      121 acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa
      181 ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat
      241 tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga
      301 tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt
      361 tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat
      421 acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt
      481 ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt cttcccacta
      541 cattctataa acaaagtata cgctacatct agattttttt aaatcattac tggat
//
LOCUS       DAZAOG010000349          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000349 DAZAOG010000000
VERSION     DAZAOG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.365"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>588
                     /locus_tag="ACHDLD_002979"
     CDS             <1..>588
                     /locus_tag="ACHDLD_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3364611.1"
                     /translation="LRKCVDSLLSQTLKEIEIVLVDDGSPDASGEIADEYQKKYSNVK
                     TIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYLVASKENADIVVSGH
                     CDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKDQEVEAFPMSVCMSLYNK
                     NLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEY"
BASE COUNT          225 a           61 c          128 g          174 t
ORIGIN      
        1 ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga aatcgtatta
       61 gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca aaaaaaatat
      121 tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag aaatacagga
      181 attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg ggtagaaagt
      241 gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat agtagttagt
      301 ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc tttgagtggg
      361 aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt tggacatagt
      421 ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt gtataataaa
      481 aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga tacaattttt
      541 aacttgaatg catatgatgt cgcaaataaa attgctttta cagaatat
//
LOCUS       DAZAOG010000350          579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000350 DAZAOG010000000
VERSION     DAZAOG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.366"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>579
                     /locus_tag="ACHDLD_002980"
     CDS             <1..>579
                     /locus_tag="ACHDLD_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364612.1"
                     /translation="HDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIE
                     LLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGIT
                     DDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDIL
                     TIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLK"
BASE COUNT          183 a           57 c          108 g          231 t
ORIGIN      
        1 catgatttaa cttttgcgat gggggttttt ttcctttatt acgtattttt tgaaaataaa
       61 aatacaaaaa aacataagat aaaaattatc gtatctttaa ttttaatttt catgggattg
      121 aagagaattg aactgttagc tttaagtggt gcaattctag tgtattatgt tttattaaag
      181 tggggaaaaa agattcaaat ccgtgcatta gtgtgtacta tttgtgcaac tgttatttcg
      241 ttagtgttta tttacattat agatacagga attttggcat tgcttatgtc aaatttgggc
      301 attacagatg acggtgcgag gctttcattt tatctatatt catctaaatt ttatgaactt
      361 ggtttaggat atgtaggaac aggatggaca tggttttcaa aatacttttt taatctttat
      421 ttatcgcact ttaggttaaa tggtggaaga attgcagcaa gcctacacag tgatatatta
      481 actatttata tagaagttgg atgtatagtt tttattatat ggatgttttg gttatttttt
      541 gtgaaaccga taagactaac taaaaaattt ggattgaaa
//
LOCUS       DAZAOG010000351          576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000351 DAZAOG010000000
VERSION     DAZAOG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.367"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            320..>576
                     /locus_tag="ACHDLD_002981"
     CDS             320..>576
                     /locus_tag="ACHDLD_002981"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3364613.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDK"
BASE COUNT          207 a           62 c           97 g          210 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaa
//
LOCUS       DAZAOG010000352          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000352 DAZAOG010000000
VERSION     DAZAOG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.368"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>575)
                     /locus_tag="ACHDLD_002982"
     CDS             complement(<1..>575)
                     /locus_tag="ACHDLD_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3364614.1"
                     /translation="GLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQNMQENMAA
                     YANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLSVTPAIFN
                     ISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWTTPPVISG
                     FLATAGDWRAAVLQILIIAASVFIYLPFLRI"
BASE COUNT          213 a          106 c           92 g          164 t
ORIGIN      
        1 caattcgtaa aaacggtaaa tatataaaca cacttgcggc tataattaat atttgtaaaa
       61 cagctgctcg ccagtctccg gctgttgcta aaaatcccga tataactggt ggcgtagtcc
      121 aaggaatttg cactactaca tgattaatta atcccacagc agttgcaaaa tatgctactg
      181 tcaaagaaaa aatcggtgca agcacaaagg gaataatcag actgatatta aatacaatag
      241 gcaatccaaa aataataggt tcgcttatat tgaatatagc gggagttacc gataatttag
      301 caaagtcctt ataatctttt ctacgactga agataaatat tgcaatcaat aaagcaatcg
      361 tatttcccga tccacccatt accgcaaagg tatctttaaa ggccatattg ataatatgtg
      421 gaatttgctc tttgtttgca taagctgcca tattttcttg catattctga atcaaaaacg
      481 gatctaacaa tgaacctgat attactgctt gatggatacc aaatccaaaa aatagattag
      541 caatagatgt aattaacaaa aatcctggta aacca
//
LOCUS       DAZAOG010000353          571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000353 DAZAOG010000000
VERSION     DAZAOG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.369"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..>571)
                     /locus_tag="ACHDLD_002983"
     CDS             complement(27..>571)
                     /locus_tag="ACHDLD_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFN3364615.1"
                     /translation="NHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHS
                     SSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTG
                     NYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKL
                     IKAVHELYMKKGYENKTKRM"
BASE COUNT          173 a           91 c           98 g          209 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg a
//
LOCUS       DAZAOG010000354          535 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000354 DAZAOG010000000
VERSION     DAZAOG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.370"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>535
                     /locus_tag="ACHDLD_002984"
     CDS             <1..>535
                     /locus_tag="ACHDLD_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364616.1"
                     /translation="IFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYKG
                     DFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKS
                     NLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDS
                     YYWEEIGLYAPQIEGED"
BASE COUNT          212 a           57 c           97 g          169 t
ORIGIN      
        1 gtatttttgt cttattatta catggaagag ttattaactt agttaatcga aaaagtacga
       61 ctaaatttgc aaactcattc tggtttttaa aaaatattaa taaaacagtg gaaaagaatg
      121 gagtttataa aggtgatttt cattttcaaa atgctgagac tattgaacga gaacatcatt
      181 atttcgattt attagtgaat acagtggtag aattagcgaa atgggcaaag tcaaacgaaa
      241 cttatagtca agtacttaaa agaattatga gtcaagaaac ttcatttatc tatgagttaa
      301 gcaagtcaaa tttgaaatat acttataatg aagatgaact tgaaatttta gataatccag
      361 aaaattcaca atggattaaa gagttgaaga gagaagactt tattgagaca ctaagaattc
      421 taaaatcgtt atttgatttg aaattagcca aagctaccat catgggaatt gaaagagatt
      481 catattattg ggaggaaatt ggactatatg ctccccagat tgaaggtgaa gataa
//
LOCUS       DAZAOG010000355          528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000355 DAZAOG010000000
VERSION     DAZAOG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.371"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..466)
                     /locus_tag="ACHDLD_002985"
     CDS             complement(275..466)
                     /locus_tag="ACHDLD_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364617.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          159 a          110 c           75 g          184 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaat
//
LOCUS       DAZAOG010000356          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000356 DAZAOG010000000
VERSION     DAZAOG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.372"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHDLD_002986"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHDLD_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN3364618.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZAOG010000357          515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000357 DAZAOG010000000
VERSION     DAZAOG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.373"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>515)
                     /locus_tag="ACHDLD_002987"
     CDS             complement(107..>515)
                     /locus_tag="ACHDLD_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN3364619.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DAZAOG010000358          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000358 DAZAOG010000000
VERSION     DAZAOG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.374"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..248)
                     /locus_tag="ACHDLD_002988"
     CDS             complement(<1..248)
                     /locus_tag="ACHDLD_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFN3364620.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCS"
BASE COUNT          148 a           93 c           71 g          202 t
ORIGIN      
        1 gaacattttc cattaatttc cagacatata tccaattcac tgacggttac tcgcaaattt
       61 ttctctagat cgaacttttg taaatcatgt ttcagcttat cgatcgtttt tttcaagaga
      121 aatgtcgata gcgacaactc ttcttgaaca ttcgatattg actctgataa atttgagctt
      181 gcttctaaat atttaagtaa tgaataactt ctgtgatctt ctttgcttaa aaaaaactca
      241 taattcatta gtcatcagct ctttctacgt atattacttt ccatgcattc accaactagc
      301 tggggataaa actgatttcc tggatccgag tcactgttgt cagcgattct tgtttgtata
      361 atatacatcg tcctgtcttt tttttaacat tttggagagc gttttatttg gcctttctcg
      421 cgatcaaacc tgttattaga attatatcat ttactagtat gcatttttat taataaaatg
      481 gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DAZAOG010000359          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000359 DAZAOG010000000
VERSION     DAZAOG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.375"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..512
                     /locus_tag="ACHDLD_002989"
     CDS             141..512
                     /locus_tag="ACHDLD_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364621.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          199 a           63 c           89 g          163 t
ORIGIN      
        1 tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct cagattacga
       61 tgttttggat actctcgata atgtattatc tgactataaa tatatgggga aatctgtagt
      121 agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt tccaaacaaa
      181 ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct gtttcaagag
      241 catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat gatattattt
      301 ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt tcaacagtta
      361 aggaattgaa taccttagga atacataata gtgggtatag aatgttagag aaaagaaaat
      421 gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg atgaatcgag
      481 aagcagaaaa aataataaac aaactcacct aatg
//
LOCUS       DAZAOG010000360          507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000360 DAZAOG010000000
VERSION     DAZAOG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.376"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>507)
                     /locus_tag="ACHDLD_002990"
     CDS             complement(<1..>507)
                     /locus_tag="ACHDLD_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364622.1"
                     /translation="DKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKSEFTLGHVEK
                     LISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIIDSLGEMRSYN
                     YLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTEYGNEIFAKY
                     DEILKNYLD"
BASE COUNT          161 a          100 c           50 g          196 t
ORIGIN      
        1 gtctaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc catattccgt
       61 ccttaaaatt atattttcag tgccagacaa gtgatttata ttgagttgat aaaaaagatc
      121 tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca ttccatcagc
      181 tctatgtgtt acatacaaat aattgtatga cctcatttca cctaatgaat caattatttc
      241 ttgcccttta gctctaatat attgatctct attacctatc tcactcttat ttaacaaaag
      301 ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc tactactaat
      361 ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat tcgctaaata
      421 ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc tacatcgatt
      481 ctctatctct tcaaaaagca tcttatc
//
LOCUS       DAZAOG010000361          485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000361 DAZAOG010000000
VERSION     DAZAOG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.378"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACHDLD_002991"
     CDS             complement(62..259)
                     /locus_tag="ACHDLD_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3364623.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAZAOG010000362          482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000362 DAZAOG010000000
VERSION     DAZAOG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.380"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>482
                     /locus_tag="ACHDLD_002992"
     CDS             <1..>482
                     /locus_tag="ACHDLD_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3364624.1"
                     /translation="DFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWLY
                     RKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFV
                     VLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKE
                     "
BASE COUNT          160 a           49 c           77 g          196 t
ORIGIN      
        1 gatttcagat ctattattac ttccatggtt atgacgtata tgttttcatt tgaagggata
       61 aaagggtatg gttatgtagt attccagata atgcttgatt ctttgttttt gctgatgttt
      121 atttggttat atagaaaatt agctataaat aggattgttg aacagtccag ttctaaactt
      181 actgctatat tcatgattta tttacttaca attggtttat ttgtttctta tgttgctcat
      241 caatatcaag tatttgatca ttttgtgttt ggtgtcttaa tatttttaat cattcaaatg
      301 tttgttgttc tatttttatt tattagaata actattaggc aacgagaaca atatgaacag
      361 caattgaaaa gaaaagaatt ggtttatcta aaaaaatata ctgattcact ggaaaaagat
      421 caagaaaaat ttgccagatt tagacatgac tataaaaatt tattgctgag tttaaaagaa
      481 at
//
LOCUS       DAZAOG010000363          482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000363 DAZAOG010000000
VERSION     DAZAOG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.381"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           95 c           56 g          172 t
ORIGIN      
        1 taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa
       61 aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta
      121 atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat
      181 agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc
      241 ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa
      301 ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga catctcgatt
      361 catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata
      421 aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa
      481 tg
//
LOCUS       DAZAOG010000364          440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000364 DAZAOG010000000
VERSION     DAZAOG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.382"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..385
                     /locus_tag="ACHDLD_002993"
     CDS             2..385
                     /locus_tag="ACHDLD_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364625.1"
                     /translation="MSEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          162 a           61 c           88 g          129 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc
//
LOCUS       DAZAOG010000365          436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000365 DAZAOG010000000
VERSION     DAZAOG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.383"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>436)
                     /locus_tag="ACHDLD_002994"
     CDS             complement(<1..>436)
                     /locus_tag="ACHDLD_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFN3364626.1"
                     /translation="LNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKA
                     NIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLND
                     LSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERI"
BASE COUNT          147 a           75 c           56 g          158 t
ORIGIN      
        1 gaattctttc aaatgcttga gatactccgt ctttatcatt tgaaacagtg acataatcag
       61 cttttttcat gatttcttga tgactatttc ccattgcaaa actaatatct gcactttcaa
      121 acatagataa gtcatttaaa ctatctccga tcgcgattct caaaggtttc tttaaattgt
      181 agattgattc caaacgatca aacaccacac ctttagatac attttgaggt acgatttcaa
      241 tatttacagg tgccgattca gtaacagaca catttagcat ctttttcaaa tggttaaata
      301 tattagcttt caattgacga ttaggaataa aaataaaaaa tttgtaaatg ctgatagcat
      361 ccttttctat aatttcctca atattattat aagggattga acaaatagat ttttcatact
      421 caagctcata gtttag
//
LOCUS       DAZAOG010000366          399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000366 DAZAOG010000000
VERSION     DAZAOG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.384"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAZAOG010000367          397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000367 DAZAOG010000000
VERSION     DAZAOG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.385"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>397
                     /locus_tag="ACHDLD_002995"
     CDS             <1..>397
                     /locus_tag="ACHDLD_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN3364627.1"
                     /translation="NKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESI
                     ELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTE
                     IDSQSSLLTDTLNLYFTSSWYSIKREKLIQ"
BASE COUNT          141 a           48 c           73 g          135 t
ORIGIN      
        1 aataaattga atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg
       61 aatctaggtt ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata
      121 ggagagagta ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt
      181 agagaacaac aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta
      241 gaattttctt ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa
      301 acagaaattg attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca
      361 agctggtatt ctattaagcg tgaaaaatta atacaga
//
LOCUS       DAZAOG010000368          390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000368 DAZAOG010000000
VERSION     DAZAOG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.386"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..>390)
                     /locus_tag="ACHDLD_002996"
     CDS             complement(100..>390)
                     /locus_tag="ACHDLD_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364628.1"
                     /translation="VGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
BASE COUNT          161 a           68 c           52 g          109 t
ORIGIN      
        1 attgatttga gatcttttta tacttcaaaa aagataacac ccaactttca aaaatatgtt
       61 ttggaagttg ggacaaacga gttaatacat tttaatacat tattttttat tcaaaaaaaa
      121 tagcacaatg gcaaagatcg taagagagat cataagcccc aatgaatgac taaaaagaaa
      181 agcagaatca catgccatat catcccatat ttcttcaaga aaataaataa gcgggaaaaa
      241 gggaaggaat agtagaacat cgatccagca ccattttttt cttcttttga aataacataa
      301 aacaataaac atgaacgtgc agggaagtaa aaacaaagct ttactcataa ctaacaaaat
      361 accagctcct tttatttttt tctcccccac
//
LOCUS       DAZAOG010000369          382 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000369 DAZAOG010000000
VERSION     DAZAOG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.387"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>382
                     /locus_tag="ACHDLD_002997"
     CDS             <1..>382
                     /locus_tag="ACHDLD_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364629.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMF"
BASE COUNT          150 a           70 c           58 g          104 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt ta
//
LOCUS       DAZAOG010000370          375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000370 DAZAOG010000000
VERSION     DAZAOG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.388"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHDLD_002998"
     CDS             complement(<1..132)
                     /locus_tag="ACHDLD_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN3364630.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAZAOG010000371          366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000371 DAZAOG010000000
VERSION     DAZAOG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.389"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..>366
                     /locus_tag="ACHDLD_002999"
     CDS             97..>366
                     /locus_tag="ACHDLD_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364631.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAF"
BASE COUNT          169 a           30 c           63 g          104 t
ORIGIN      
        1 agtatatatt gaaaatacaa gatgaaggta aaaatcatgc tataataaaa tagtcaaaat
       61 ttttgccgat atttcagttt aagaggagag attgaattga aattaagagc aaacataatt
      121 gaaaaaagag gaacattaca agtagttgta tcttataaag actctaacgg aaaaattaga
      181 caaaagtgga aagatacaaa attaaaaaag aaaggaaata aaaaacgagc agaaaaaatt
      241 agagatgaat ttttactaga attggaaagt caattagcta tggaatgtga atgtgctaat
      301 ccaaatttat tgttttatga ctatttgctt aattatttgg aagtatcaaa aaaacaagta
      361 gcattt
//
LOCUS       DAZAOG010000372          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000372 DAZAOG010000000
VERSION     DAZAOG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.392"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           65 c           56 g          111 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttct
//
LOCUS       DAZAOG010000373          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000373 DAZAOG010000000
VERSION     DAZAOG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.393"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHDLD_003000"
     CDS             complement(<1..321)
                     /locus_tag="ACHDLD_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3364632.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DAZAOG010000374          343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000374 DAZAOG010000000
VERSION     DAZAOG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.394"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHDLD_003001"
     CDS             <1..156
                     /locus_tag="ACHDLD_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN3364633.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>343
                     /locus_tag="ACHDLD_003002"
     CDS             175..>343
                     /locus_tag="ACHDLD_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3364634.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSIS"
BASE COUNT          139 a           30 c           65 g          109 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caa
//
LOCUS       DAZAOG010000375          341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000375 DAZAOG010000000
VERSION     DAZAOG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.395"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACHDLD_003003"
     CDS             <1..>341
                     /locus_tag="ACHDLD_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN3364635.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZAOG010000376          338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000376 DAZAOG010000000
VERSION     DAZAOG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.396"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..338
                     /locus_tag="ACHDLD_003004"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACHDLD_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAZAOG010000377          331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000377 DAZAOG010000000
VERSION     DAZAOG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.398"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACHDLD_003005"
     CDS             <1..>331
                     /locus_tag="ACHDLD_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFN3364636.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAZAOG010000378          326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000378 DAZAOG010000000
VERSION     DAZAOG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.399"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..>326)
                     /locus_tag="ACHDLD_003006"
     CDS             complement(203..>326)
                     /locus_tag="ACHDLD_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3364637.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          119 a           46 c           37 g          124 t
ORIGIN      
        1 tatatgtatt aaatctaaaa caaccatttc atgaaaaata acatcctttc tatcactatt
       61 agattcatat ctcttttttt gagttaaacc agaaataact ttttcaaaat aaaattttaa
      121 cgccaatcca tgagtcaatt caaaaaaaga aaaaagattc tttgttttct cagaagttca
      181 attctttatt atttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc
      241 tcaaaaatat ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt
      301 gattgctgac actggaaata aatgtc
//
LOCUS       DAZAOG010000379          324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000379 DAZAOG010000000
VERSION     DAZAOG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.400"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..>324
                     /locus_tag="ACHDLD_003007"
     CDS             71..>324
                     /locus_tag="ACHDLD_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364638.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           95 a           57 c           62 g          110 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg atac
//
LOCUS       DAZAOG010000380          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000380 DAZAOG010000000
VERSION     DAZAOG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.402"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHDLD_003008"
     CDS             complement(<1..>321)
                     /locus_tag="ACHDLD_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364639.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZAOG010000381          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000381 DAZAOG010000000
VERSION     DAZAOG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.404"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHDLD_003009"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHDLD_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZAOG010000382          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000382 DAZAOG010000000
VERSION     DAZAOG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.406"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHDLD_003010"
     CDS             complement(76..>308)
                     /locus_tag="ACHDLD_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3364640.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAZAOG010000383          302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000383 DAZAOG010000000
VERSION     DAZAOG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.407"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACHDLD_003011"
     CDS             <1..>302
                     /locus_tag="ACHDLD_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3364641.1"
                     /translation="AAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRN
                     NYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILR"
BASE COUNT          110 a           34 c           54 g          104 t
ORIGIN      
        1 gctgctccac tgaggtataa aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa
       61 aaagtatatt ctgtaagagc tatttgttct acttatcttc ctgattggag aaaaggtact
      121 gattatagaa ataattattc tgcctttaaa tctatggggg gaggagtaga attagactgt
      181 atacatgaat tggattatat tataaaccta tttggtttcc ctaaatcagt aaaaaaacag
      241 tttggtaaat attcgaatct tgaaattaac agtaatgatt tagcaacata tatattgaga
      301 ta
//
LOCUS       DAZAOG010000384          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000384 DAZAOG010000000
VERSION     DAZAOG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.408"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..272
                     /locus_tag="ACHDLD_003012"
                     /pseudo
     CDS             162..272
                     /locus_tag="ACHDLD_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          104 a           47 c           38 g          111 t
ORIGIN      
        1 acatctttca gcattttact atatttggta tatagtgcta aggttcatat tcgattgaca
       61 agagaatata aattgatcta tttctatttc ctaagttgag taaacataac tttgcccttt
      121 ataaatatct tccctctctc tcttctctct atattttata tttgaataat catatcaagg
      181 tattccaacg aaatgccaat ggatttcgaa gcttttataa ctttaaatta cggatcatga
      241 tttggcatgg ggaatcatta ctaataaaat aaaaaaatga acagaaagaa aacagttctt
//
LOCUS       DAZAOG010000385          286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000385 DAZAOG010000000
VERSION     DAZAOG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.410"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>286
                     /locus_tag="ACHDLD_003013"
     CDS             <1..>286
                     /locus_tag="ACHDLD_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFN3364642.1"
                     /translation="QVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSYGRNR
                     RVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIE"
BASE COUNT          107 a           40 c           59 g           80 t
ORIGIN      
        1 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
       61 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
      121 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
      181 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
      241 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaaga
//
LOCUS       DAZAOG010000386          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000386 DAZAOG010000000
VERSION     DAZAOG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.411"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHDLD_003014"
     CDS             <1..>282
                     /locus_tag="ACHDLD_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN3364643.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZAOG010000387          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000387 DAZAOG010000000
VERSION     DAZAOG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.414"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHDLD_003015"
     CDS             <1..>270
                     /locus_tag="ACHDLD_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3364644.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAZAOG010000388          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000388 DAZAOG010000000
VERSION     DAZAOG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.415"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          102 a           28 c           40 g          100 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt
//
LOCUS       DAZAOG010000389          265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000389 DAZAOG010000000
VERSION     DAZAOG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.417"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           38 c           41 g           75 t
ORIGIN      
        1 tattaaacaa cgttgtcgcg cttcattgaa acattttatg aaaattccaa aaaagagttg
       61 acacatgttt ttagacgtga taagataact aagtcgttaa aaagaaccac ttgcgaagct
      121 tgattctatc gaaaaaaaac ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg
      181 ataaatgagt tgctgaaaga tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt
      241 tgacaatcgc ttgtctctct gatat
//
LOCUS       DAZAOG010000390          256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000390 DAZAOG010000000
VERSION     DAZAOG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.denovo.418"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>256)
                     /locus_tag="ACHDLD_003016"
     CDS             complement(<1..>256)
                     /locus_tag="ACHDLD_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364645.1"
                     /translation="AALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNV
                     GAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYIT"
BASE COUNT          105 a           37 c           35 g           79 t
ORIGIN      
        1 gtgtaatata aaatatttgt acgataaaat tcatcatata aaaaacacga atcgtttttg
       61 ttatttttga aattaataca ctaattaaac ttaaaataat agaagtatat ccaaacatag
      121 ctccaacatt tggacttgta tagacaccaa ataaccgatg ttcaataaaa ccttgtcttg
      181 caagtaatcc tggcgttcct gttgggacat gataagaaat caaaaaaata aattgataga
      241 tagatattag cgcagc
//
LOCUS       DAZAOG010000391         5820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000391 DAZAOG010000000
VERSION     DAZAOG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.151"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1058
                     /locus_tag="ACHDLD_003017"
     CDS             162..1058
                     /locus_tag="ACHDLD_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3364646.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1208..2149)
                     /locus_tag="ACHDLD_003018"
     CDS             complement(1208..2149)
                     /locus_tag="ACHDLD_003018"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3364647.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2275..3396
                     /locus_tag="ACHDLD_003019"
     CDS             2275..3396
                     /locus_tag="ACHDLD_003019"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3364648.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3654..5156
                     /gene="eat(A)"
                     /locus_tag="ACHDLD_003020"
     CDS             3654..5156
                     /gene="eat(A)"
                     /locus_tag="ACHDLD_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3364649.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5223..5750
                     /locus_tag="ACHDLD_003021"
     CDS             5223..5750
                     /locus_tag="ACHDLD_003021"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3364650.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1906 a         1027 c         1225 g         1662 t
ORIGIN      
        1 agaaaacttt taggcgaatt ttaatagaag aatgatttat ttcatcatat tttcatacaa
       61 atgttaaatt ttcatacaaa tataggaaac gttttactgg ctttttacta tttaaacagg
      121 ctatcatagg aaaaagtgac agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac
      181 tgctagttgc aaaaaataaa tcactttatt ttttgccttc catccgctct ttagatgttt
      241 ggtccggaaa agatttacta cagtcggaat tcattaaatc agctagacaa cagttggcaa
      301 attatcagct gatcgttttt ttagattatg gatttgatat ggagatggct cgacgcgtgc
      361 gcccatttac caatgcaaaa attgtgttat ttttttggaa tcacttcaaa gcagaacacg
      421 aggcgatgct gaagcaggca caagatgaac cggcaatcga tgagatttat cattttgatt
      481 ttttggaagc aaaagaactt gggctgaaac ataatagttc attttattcg aaacatatga
      541 atttttcttc tagcgaacca aagacggatc tattttttgg tgcaacgaat aatgggagaa
      601 aagaacgagc agaaagctat aagcaggaat ttataaaaag agatgttacg accaactatt
      661 tcattcttcc acatcgaggg aatgaacaac ctggatatct tacttatgaa gagtacctga
      721 aaaaaacagg ggaatcgcgc gggattctgg aactgttgcg ggagggtcag caaggagtga
      781 ctttgcggac atttgaatcc atgtattttc aaaagaaact agtgacggat aatcaagcga
      841 ttgctcatta tcatttttat cgtcctgaaa atattttttt gcttcgagaa cgtgatttag
      901 atgaattacc ggaatttctg catacaccat ttaagccaat cctatcagag ctattggaat
      961 tttatgatgc agaaaattgg gcaaaacgat ttatcactaa cgattccact atttatgagc
     1021 aatacgaata tgacctctcc tctgttgaaa acaaataaaa aacctacatt ttgaagagat
     1081 tatgtactga aacaaacaag cacaattctt atggcatcat gagagttgtg ttttcttttt
     1141 gatcttttat tgggcttaaa aaaagagaac cagctgctga ttcagctgat tctcatcaga
     1201 gagcctttta ttctgcgcca aggattcctt cttttgcatg gactaattgg gtcaaaaagg
     1261 cacaatatgg tgtagcaata tcatattttt gtccttttcg ccaaatggca ccattgatgt
     1321 agtcgatttc agtcaaacga tggtttttga tcaggtcttg gtacatagat gggtaatgca
     1381 acccgatgtt gtcaggatca taagtagaag caatatgttg gtacacttct tcttgatcta
     1441 gtatgattcc ttcttttgct gcgatagaag caaattcaga tacgattgtt cgaaccatgc
     1501 tctctgccgc tttttgcgcg cctagttcag caatattaca atccaaaatc gtgcataaac
     1561 cattcaatgt tccatttaca caagcttttc gccaaatgga ataacgaacg ttatggctat
     1621 agatcggatt taaatttgct tcttgaaaga cttctacaac tttttttgta aatgcttcgc
     1681 cttctggttc caagttctct agttcgattt ctccatcacc taacaatgtg acttttccgg
     1741 gaccagctag accagctgtc cacatcgtga tcccaaataa gatatttttc tttggcacat
     1801 atttttccag tacatcttca tgacctaatc catttagtaa acacaaaaca taagtattgt
     1861 cagtaataat ggattggatc gaacaaaaca tatcatctaa ttgattggct tttgttaatg
     1921 ctacgatcaa gtccacgtgt tcgttgcttt cgatgatttc ttctggtgaa tagattggca
     1981 gttttgcgac cacttcttta ccgttaaagt cagcaatcaa cccatttgta cgaatggctt
     2041 caatgtgagc aggccaacga tcgatcaaga ggacctcatt tccgctttga tgtagcatca
     2101 atccgattcg gctgcccatt gctccggccc cagctattgc gattttcatg ttttttctct
     2161 cctttattca atgagttccc ttttagtata tagaaaaatg gaagaaaata gcattgtttt
     2221 gtttgaaaaa aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat
     2281 attttggcgg caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca
     2341 gttctaaatg gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc
     2401 ggagaaaata ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat
     2461 gggatggatc tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga
     2521 aaaaacaccg cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca
     2581 acggatcaaa ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca
     2641 ctgcttcaag aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt
     2701 ggtctaggat tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct
     2761 ttattatcta ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa
     2821 ttgattatcg ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa
     2881 tttggaagac aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga
     2941 aagattgctc agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt
     3001 ggagcagcag gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt
     3061 ttcgatttag tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc
     3121 attacaggag aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta
     3181 gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa
     3241 gagctagaag gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata
     3301 ggtttagaag aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag
     3361 acacttagca gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc
     3421 tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat
     3481 tgatgaaagc tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc
     3541 gggattggct tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc
     3601 tctttagcca gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta
     3661 aaatcgaaat aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac
     3721 aagcaaatct aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag
     3781 gaaagacaac tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc
     3841 atcagcaaga atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt
     3901 acgtgttaaa tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga
     3961 tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga
     4021 caaaagtcct actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac
     4081 caacgaatca tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa
     4141 aacaaggctt catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg
     4201 ttttagcaat cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag
     4261 aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag
     4321 tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag
     4381 ataaaacaaa gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag
     4441 cagtgggtgc tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg
     4501 agacccagat cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga
     4561 cgatgaatag ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag
     4621 ggtatgaaaa tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg
     4681 cgatttcagg tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat
     4741 tcaacggcaa ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa
     4801 gccaaaatta tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag
     4861 actaccaagc atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata
     4921 acaagatcga gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat
     4981 cacagccagc tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc
     5041 aagaacaatt agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg
     5101 atcaaacctt cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct
     5161 tgtaatctca agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc
     5221 acatgcttta tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg
     5281 gacgggcaga tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa
     5341 aggtagtaga tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa
     5401 gagatacagc acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat
     5461 tggtagaaat ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa
     5521 aagcacgttt agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg
     5581 cacggatcgt tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag
     5641 tttgccataa tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg
     5701 ggttctttac attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt
     5761 gtgttattta aataaaatat ttcaaggcac tgcttttgct tagcagtgcc tttttttagg
//
LOCUS       DAZAOG010000392         5607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.159, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000392 DAZAOG010000000
VERSION     DAZAOG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.159"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..590
                     /locus_tag="ACHDLD_003022"
     CDS             126..590
                     /locus_tag="ACHDLD_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3364651.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            574..1203
                     /locus_tag="ACHDLD_003023"
     CDS             574..1203
                     /locus_tag="ACHDLD_003023"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3364652.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1557..3035
                     /gene="msr(C)"
                     /locus_tag="ACHDLD_003024"
     CDS             1557..3035
                     /gene="msr(C)"
                     /locus_tag="ACHDLD_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN3364653.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3082..3528
                     /locus_tag="ACHDLD_003025"
     CDS             3082..3528
                     /locus_tag="ACHDLD_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN3364654.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3727..4242)
                     /locus_tag="ACHDLD_003026"
     CDS             complement(3727..4242)
                     /locus_tag="ACHDLD_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3364655.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4359..5561
                     /locus_tag="ACHDLD_003027"
     CDS             4359..5561
                     /locus_tag="ACHDLD_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3364656.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1743 a          936 c         1198 g         1730 t
ORIGIN      
        1 tctctatact tttctattga gagcgttttt tttattacac agctgattct ttgacttttt
       61 tcttttttta ttctattatt gacaagtcaa tcgttgacga gtcaattatt agaaaggagg
      121 aaattatgtc tttagaagag attagtcacc ttttatatca aatcaaatta gccgatcaaa
      181 aaatcgtcca gatctttgaa gaaaatatcg gtataagtct tactagatac gagatcatga
      241 tgagactgaa agaagaaaat ccattgctac aaagcagttt gcaagaaaag ttgcagattg
      301 atcaaggagc agttactcgt catctgaagg tattggaagc aaatggctat gtttcgcgtc
      361 agagaaatcc tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga
      421 aaatcaagca ttgtgaaatg gaccataaag atacaggttc cgtgctatcc ccggctttca
      481 gcgatgtaga aatgaaacaa tttctaacac ttttagataa attgaatacc caactttgca
      541 atataacaac agaaacgaaa gaggttaata ataatggaac aaaaaaatga tttttcaaca
      601 attatgactg gtagaagatc cgttcgcgta tatgatgaaa catacaaaat cccacacgaa
      661 gaaatgctgg aaatgatcaa agaagcgacg actgcacctt cttctgtcaa tatgaaacca
      721 tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt
      781 ttcaatacga gacaaaatga tacttcttct gcaatggtca tgatttttgg cgatatgcaa
      841 tgttacgaat atggcgaaga aatctacaac caagcttacg aatcagggaa gatgccaaaa
      901 gaagtcagag atcaacaatt agctgcgatc attccttatt acaagagttt ttcaagagaa
      961 gaaatgaacg atgtagtcaa agtcgattct agcttagcgg ctatgcaatt catgttagtt
     1021 gcgcgtgatc atggctatga aacaaatcca attggtggat tcgaagcaga tcagttggca
     1081 gaggcatttg gtttggataa agaccgttat gttccagtta ttattttatc aataggaaaa
     1141 gcggtagaag aaggttacga gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa
     1201 taaaaggttt tcagttacta ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc
     1261 taataaaaaa ggaggtcgga aaaatgactg catcgatgaa attacgtttc gaacttttga
     1321 ataacaacta aataaattca aaagacatgt ataaacaaca atttcatgga tggagtatgt
     1381 ccattttttc gaacgacagt agaataggtg aaagctatga cagatttggt gatttagtca
     1441 tagttatttc gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc
     1501 tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta aaacatatgg
     1561 aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag ctagaactat
     1621 ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga gaaaatggtg
     1681 tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat catgggaaaa
     1741 tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct gaagcaaagg
     1801 acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact tcagagcgga
     1861 aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct tcttatgaac
     1921 aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt gatcgactga
     1981 ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat cgctattttc
     2041 tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga gagttttcgg
     2101 ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag ctacgagaag
     2161 cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac
     2221 aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc agactggatc
     2281 ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa
     2341 cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag atgaaacaaa
     2401 tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc atgggacaaa
     2461 atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt cagttttctt
     2521 taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc ttattagaac
     2581 atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt caagtatatc
     2641 agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc atgagacaaa
     2701 cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt gctcaggaaa
     2761 ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg acgattgctt
     2821 tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat tttattgata
     2881 tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg attttgttta
     2941 cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa ataaaagggc
     3001 agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca
     3061 aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat ttttacaaca
     3121 atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct gcatcaagat
     3181 cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc tttcattttg
     3241 gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc agtaaatcca
     3301 gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg aatggtgaca
     3361 tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt attctctgct
     3421 tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt
     3481 ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg gaacactgtt
     3541 atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaatgcag ttctagaaaa
     3601 atagtgatcc gaacgatagg ttttggcctg tcgttcggat cgctttttct tttccaactg
     3661 tattgctctt ttattgaaga taatttgctc ttcaatagat atttctagaa aggttctgtt
     3721 tatcccttac tcaaaaaact gctgcatttg ttctcttact tgctgacacc attggagttc
     3781 ctgctgacaa agcataattc tgcgctggat cacaacataa cgtccaaatt cttgtttctt
     3841 gtctagcgag aggtcattca attttttttg atttttttgc atgattttgt tcagctgttg
     3901 ttctctagtg aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa aagcatagat
     3961 ttttgctaga aattcatctt tttgcgtagg agcgggagtt tcctctttga tccattcctc
     4021 tacaagagca cgtccttcat cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt
     4081 gtccaaaacg gtcacataac cctgctggtc taacttggca agcgttgtat agatctgact
     4141 gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag aacgtggttt
     4201 ggataaaagc acgcataata atatataggc taaagtattc ataaaaatcc tacctctcgt
     4261 cttattttct ttattatatc acagtaaagt ttccacgttt tacatgtgca tattgacatg
     4321 taaaataaat catgtatgat gctaatagag agaaagggat ggaaatattg gagaatcaaa
     4381 tagatataca agaacaaaca aatgaagaaa caaaggggaa aagttcagcg ctagttgtag
     4441 cagctacctg tatgatcgct tttatgggag tgggtctagt agacccaatc ttaaaaacga
     4501 ttgctgcaca gttaaacgct acgccagctg aaacgaccct gttatttact agttacatgt
     4561 tagtcactgg tgtggtgatg ctatttacag gtttcttatc tagtcggatc ggtttgaaaa
     4621 aaacattgat gacaggattg tttatcatcg ttgtattcgc tggtcttggc ggattatctg
     4681 ggaatatcgg aatgctgatt gggttacgtg ccggctgggg tgtcgggaat gctttatttg
     4741 tctctactgc tttagcaacg atcgtcagta ttttagttgg taacacagaa aaagcaatca
     4801 tgatgtatga agcagcactt ggctgcggaa tggctgttgg ccctctagtc ggtggggtct
     4861 tgggcagtgg ttcatggcgt tatccatttt ttggggtagc atttttgatg ttttgcggtt
     4921 ttatagccct agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt
     4981 gggaaggtgt caaagcgtta agcaatcata aacttcgttc agttggacta atcgcattgt
     5041 tatataattt cggattcttt actttacttg cgtatgcgcc atttttattg attggctatt
     5101 ctgaattaga agtcggtttt gtattttttg gctggggtgt gcttctggct attgcttcga
     5161 ttttcgttgc accggctctt gagcggaagt ttacgactta tcggacaatt ttagcagcat
     5221 ttgtcttatt cattatttgt ttgctgttat taggagctgg aacttctatg ccagtccttg
     5281 taccgatcag cattgtgtta gcaggatttt tccaaggaat tatcaataca ctgttgacga
     5341 cgattgctat ggaaatacca ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg
     5401 tccgcttttt cggtggagct ttagcaccat ttattgcagg tgtgattggt gaacatattg
     5461 gtgaaagcta tgcgttttat tttgcaggtg ccattgttct tatcagtgta tgttgtatca
     5521 tttatcaccg taattatttt gctacaaatg aagaagaata agaaatcaga ggttgtgaaa
     5581 aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DAZAOG010000393         3567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.206, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000393 DAZAOG010000000
VERSION     DAZAOG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.206"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1003
                     /gene="rlmN"
                     /locus_tag="ACHDLD_003028"
     CDS             <1..1003
                     /gene="rlmN"
                     /locus_tag="ACHDLD_003028"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN3364657.1"
                     /translation="EKKFRAAQVWEWLYQKRVSNFTEMTNLSKQLIEKLSEHFIINPL
                     KQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGLSVCVTTQVGCNIGCTFCASGLL
                     KKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVVMGIGEPFDNYDNVMDFLHIIND
                     AKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAISLHAPNNEVRTSIMRINRSFPI
                     EKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQQLADLLKDKKKLAYVNLIPYNP
                     VSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHGTDIDAACGQLRSKQMKKTVAK"
     gene            993..1199
                     /locus_tag="ACHDLD_003029"
     CDS             993..1199
                     /locus_tag="ACHDLD_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364658.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1196..2299)
                     /locus_tag="ACHDLD_003030"
     CDS             complement(1196..2299)
                     /locus_tag="ACHDLD_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3364659.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2852..3400
                     /gene="aac(6')"
                     /locus_tag="ACHDLD_003031"
     CDS             2852..3400
                     /gene="aac(6')"
                     /locus_tag="ACHDLD_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN3364660.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1163 a          620 c          717 g         1067 t
ORIGIN      
        1 cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa
       61 tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat
      121 catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt
      181 gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt
      241 gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg
      301 gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt
      361 ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg
      421 gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga
      481 tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca
      541 caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc
      601 gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa
      661 attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata
      721 tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt
      781 aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca
      841 tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa
      901 aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg
      961 acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt
     1021 tttcaattac cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga
     1081 gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc
     1141 aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac
     1201 tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa
     1261 tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg
     1321 ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta
     1381 acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact
     1441 gctgatccat catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc
     1501 cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat
     1561 agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt
     1621 gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag
     1681 tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta
     1741 gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac
     1801 ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta
     1861 caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac
     1921 ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag
     1981 ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca
     2041 ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg
     2101 aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta
     2161 ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat
     2221 attctttgac gattccattt gctttttcat acgcatctgt tccaatggta taatttggta
     2281 agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt
     2341 aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata
     2401 agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac
     2461 aagtacaaaa acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg
     2521 attccctttg cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa
     2581 acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca
     2641 tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga
     2701 ataaagtgtt tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga
     2761 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
     2821 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
     2881 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
     2941 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
     3001 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
     3061 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
     3121 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
     3181 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
     3241 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
     3301 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
     3361 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
     3421 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga
     3481 aattccaact tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa
     3541 aagtcgctta gtttcgagta gtaagaa
//
LOCUS       DAZAOG010000394         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.221, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000394 DAZAOG010000000
VERSION     DAZAOG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.221"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1027)
                     /locus_tag="ACHDLD_003032"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ACHDLD_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACHDLD_003033"
     CDS             1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACHDLD_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN3364661.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ACHDLD_003034"
     CDS             1852..3006
                     /locus_tag="ACHDLD_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFN3364662.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          989 a          631 c          663 g          938 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt a
//
LOCUS       DAZAOG010000395         2512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000395 DAZAOG010000000
VERSION     DAZAOG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.237"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..574)
                     /locus_tag="ACHDLD_003035"
     CDS             complement(<1..574)
                     /locus_tag="ACHDLD_003035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3364663.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSE"
     gene            966..1025
                     /locus_tag="ACHDLD_003036"
     CDS             966..1025
                     /locus_tag="ACHDLD_003036"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFN3364664.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            1083..1814
                     /gene="erm(A)"
                     /locus_tag="ACHDLD_003037"
     CDS             1083..1814
                     /gene="erm(A)"
                     /locus_tag="ACHDLD_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFN3364665.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1940..>2512)
                     /locus_tag="ACHDLD_003038"
     CDS             complement(1940..>2512)
                     /locus_tag="ACHDLD_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HFN3364666.1"
                     /translation="MTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPKREFIYGEWLR
                     GEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIRRAIKDSLPE
                     LIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLLDIARKGYR
                     GECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          859 a          462 c          377 g          814 t
ORIGIN      
        1 gttcagatag gtacttatct gaagctaaat gctcaagcca tgatttagat gtgttgtgat
       61 tataaattct aatcgcattt tttattccaa atttgaaaca atgcggtaaa aactcttgga
      121 ttgcccctaa tttataaaca taggtaggcg gtgtctcgac ttcagataca ttatcaagaa
      181 taactattct tccttcctca ttcaacagtt ccttcatttg ctgtattacg ctggctatat
      241 catccaaatg atgaaaggtt gttcggctta caataaaatc aaacttctca ttaaaattaa
      301 gttgttctgc attcatattc agatagaccg tatttgttag ttgacgttta gatttggcaa
      361 gatcgagcat ttgattagaa atatcaatcc ctaccacttc atcataataa cttgctaatt
      421 tctccactaa caaacccgag ccacatccga tatctaatgc tctgcctttc tttggagaca
      481 tattagacac aaagaatgaa taatcattca aaagctcatt cacgaaatcg taatcttctg
      541 caaccttatc aaactgtgat tctattgtat tcaaaaagat cccccattcc tactttatcg
      601 acattctttc attacttacc actttagatg ttttttcgtt ggggataaaa cttcccttta
      661 gacaatttta tccaaagaca atacaacagt gcaactttat taaagtcact gtcctttatc
      721 gcagccttta ctttttagta aagacagtgg cttctcttat caagtttcaa aacatattat
      781 tttgaagaaa acgtccatct gaagtgtcaa gtgcaaaatt acatataaag gtttattcta
      841 aaatgaaaag atgatacaat catattcagt tacataagga ggtttcaatt atgtgcacca
      901 gtatcgcagt agtagaaatt actttatctc attcataatg aaaaaaatgg aaaggagata
      961 aaagtatggg tactttttct atatttgtta ttaataaagt tcgttatcaa ccaaatcaaa
     1021 attaattggt tataatgaac gcttaatgtc agttcattat aaccagtaag gagaaggtta
     1081 taatgaacca gaaaaaccct aaagacacgc aaaattttat tacttctaaa aagcatgtaa
     1141 aagaaatatt gaatcacacg aatatcagta aacaagacaa cgtaatagaa atcggatcag
     1201 gaaaaggaca ttttaccaaa gagctagtca aaatgagtcg atcagttact gctatagaaa
     1261 ttgatggagg cttatgtcaa gtgactaaag aagcggtaaa cccctctgag aatataaaag
     1321 tgattcaaac ggatattcta aaattttcct tcccaaaaca tataaactat aagatatatg
     1381 gtaatattcc ttataacatc agtacggata ttgtcaaaag aattaccttt gaaagtcagg
     1441 ctaaatatag ctatcttatc gttgagaagg gatttgcgaa aagattgcaa aatctgcaac
     1501 gagctttggg tttactatta atggtggaga tggatataaa aatgctcaaa aaagtaccac
     1561 cactatattt tcatcctaag ccaagtgtag actctgtatt gattgttctt gaacgacatc
     1621 aaccattgat ttcaaagaag gactacaaaa agtatcgatc ttttgtttat aagtgggtaa
     1681 accgtgaata tcgtgttctt ttcactaaaa accaattccg acaggctttg aagcatgcaa
     1741 atgtcactaa tattaataaa ctatcgaagg aacaatttct ttctattttc aatagttaca
     1801 aattgtttca ctaaattaaa gtaataaagc gttctctaat ttcacaagag gacgctttat
     1861 tcttcccaaa aattgttcaa tatttatcaa taaatcagta gttttaaaag taagcacctg
     1921 ttattgcaat aaaattagcc taattgagag aagtttctat agaatttttc atatacttaa
     1981 cgagtgcttt cacctttgaa tatagtcctt cccacttatc atcacactct ccccgatagc
     2041 cttttctagc tatatccagt aaagttacat gctctttagg taaaagaggt atagcccatt
     2101 ctgcagcgac atctttcgag gtaatttcac cagtagtcac tgtttgccac attcgagcta
     2161 gggttaaaat tacattacgc tcatcacctt ttatcccctc aattagttct ggcaaagaat
     2221 ccttaattgc tcttcgaata tctgtcaaag gtacggagac aagtatactt gaagaatcag
     2281 gaccaaatag agaaatacta ttctttcttg cttgtgctaa aacaatagcc aaatcaggat
     2341 catagcttgg ttcctgaatt tgtccattct caaattcacc cctgagccac tcaccgtata
     2401 taaattctct ttttggagga tattgccaag ggacaacttc actcctattt ataaccgtaa
     2461 cttcaagtgg tctaacagaa tccgtatttc caatctttcc tgatatagtc at
//
LOCUS       DAZAOG010000396         2433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000396 DAZAOG010000000
VERSION     DAZAOG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.239"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1008
                     /locus_tag="ACHDLD_003039"
     CDS             40..1008
                     /locus_tag="ACHDLD_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN3364667.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1001..2032
                     /gene="vanA"
                     /locus_tag="ACHDLD_003040"
     CDS             1001..2032
                     /gene="vanA"
                     /locus_tag="ACHDLD_003040"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN3364668.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2038..2403
                     /locus_tag="ACHDLD_003041"
                     /pseudo
     CDS             2038..2403
                     /locus_tag="ACHDLD_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT          717 a          465 c          609 g          642 t
ORIGIN      
        1 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
       61 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
      121 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
      181 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
      241 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
      301 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
      361 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
      421 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
      481 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
      541 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
      601 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
      661 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
      721 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
      781 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
      841 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
      901 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
      961 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     1021 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     1081 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     1141 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     1201 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     1261 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     1321 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     1381 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     1441 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     1501 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     1561 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     1621 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     1681 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     1741 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     1801 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     1861 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     1921 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     1981 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     2041 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     2101 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     2161 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     2221 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     2281 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     2341 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     2401 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAZAOG010000397         1741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.268, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000397 DAZAOG010000000
VERSION     DAZAOG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.268"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1130
                     /locus_tag="ACHDLD_003042"
     CDS             <1..1130
                     /locus_tag="ACHDLD_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HFN3364669.1"
                     /translation="LAYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRA
                     YSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALL
                     EISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERP
                     LKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIR
                     YGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYL
                     SFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTN
                     GLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1228..1416
                     /locus_tag="ACHDLD_003043"
     CDS             1228..1416
                     /locus_tag="ACHDLD_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFN3364670.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1474..>1741)
                     /locus_tag="ACHDLD_003044"
     CDS             complement(1474..>1741)
                     /locus_tag="ACHDLD_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3364671.1"
                     /translation="QVGTMIEIDPRYLTNIENKGQHPSTQVLYDLVSLLHVSIDEFFL
                     PTDNLIKSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          574 a          270 c          327 g          570 t
ORIGIN      
        1 tacttgcata tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat
       61 cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta
      121 agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa
      181 atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc
      241 ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg
      301 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
      361 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
      421 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
      481 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
      541 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
      601 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
      661 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg
      721 gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac
      781 tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag
      841 tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa
      901 atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc
      961 gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt
     1021 tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc
     1081 taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat
     1141 gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg
     1201 aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt gtgtcctatt
     1261 tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa tttcccttta
     1321 tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca
     1381 atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt
     1441 atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt
     1501 gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta
     1561 tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc
     1621 atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg
     1681 tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg
     1741 t
//
LOCUS       DAZAOG010000398         1725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.270, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000398 DAZAOG010000000
VERSION     DAZAOG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.270"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..827)
                     /locus_tag="ACHDLD_003045"
     CDS             complement(<1..827)
                     /locus_tag="ACHDLD_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364672.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFL"
     gene            920..1269
                     /locus_tag="ACHDLD_003046"
                     /pseudo
     CDS             920..1269
                     /locus_tag="ACHDLD_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1541..>1725)
                     /locus_tag="ACHDLD_003047"
     CDS             complement(1541..>1725)
                     /locus_tag="ACHDLD_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3364673.1"
                     /translation="SLNFLAEVDEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYE
                     KSLYPRKKVRQDERIN"
BASE COUNT          588 a          312 c          232 g          593 t
ORIGIN      
        1 attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaaacgg ctttccaaat
       61 gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc aggaaaccat
      121 ttattaattt catgtgcagc acgtaatgca ttccctggag ttgtcaaaga gaaaattaat
      181 tctgctaagc ttattattga aaatactgat aacttataat tatatcgtcc attgacaatc
      241 aaatataaac taactatact tgttagaaca aaaaaacagg cgttgacttg ttcttgatta
      301 gttaaaaata tcaataaagg aatacttacc caatatattt ttctatttat ttcttctcct
      361 cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg ccactgatag
      421 ttcaaggtgg ttgctctcca accggtttcc cccattactg tcaatggtat taaacagtat
      481 atagagcaaa ccaataataa atttttaaca tttattttct caaaaacata cttgcaaagt
      541 aatactacaa ttcctaacat aatcgtagaa tttaataatt tccatagcat aaagtgttta
      601 gaaaagaaca ttaagaatag ttctataata attcttgaac tccatgtctt ataatgttct
      661 atcgaagcac ccaaatagcc aagttcatct aatctacttg cataaactac gtcatccgct
      721 ctcatagtcg gcatatacat attaaagagt aaaaagatac cccaaatccc tattagtaac
      781 aagatcagtt ctttgttatt cgttattttt ctattatgtt tgttcatttt tatactcctt
      841 cttatatttc ctaatagagt ataacatagc tttaagcatt acttaaagat aacttaaaag
      901 ttatctttta aatacttaat tgttaaatac cttatttgat gcaaaagaaa tctacgatag
      961 tcaacaaaaa aattttcatt atatccagaa aaatttttct tagttaagga cctatggatt
     1021 gcagtaatgc agaattcgtc gaataaactg ccaaagacat acaatcatat tgcttttaaa
     1081 attgacgaac aagatataga ttcgtttgtc tcaaaaatcc aaatgttagg tttaactgtc
     1141 gaaccaggac gttctcgtgt gaaaggcgag gcttcttcaa tttactttta cgactatgat
     1201 aatcacctct tcgagttaca taccggtact ctccaggaac gtttaaaatc ctacaattcc
     1261 actacctgag actagtaatg actagtctct ttttatggcc tctgatcccc ccattgagcg
     1321 aatactaata gggcttcgaa ttgatctgtt caatctttaa gcaatatccc tcaaatgagt
     1381 ttggaaagtt tagtgattca tcacaaatac ttaaatacat gtagttatat atagattgag
     1441 gttcttgtga gcgtatgctc gtgtgggttc aaatcccatc gtctgcatcc atctttccaa
     1501 aacaagcggc taggacattt ttgtctcagc cgcttgtttt ctagtttatt ctttcatctt
     1561 gtcggacctt ttttcttggg tatagacttt tctcatattc taccacaatt ttgggtttcc
     1621 ctaacacatg ataggatctc acaacaaatt gctttctatt attataataa cctgtaacga
     1681 ctaacttcat cccttcgtct acttcagcta ggaagttcaa actat
//
LOCUS       DAZAOG010000399         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.284, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000399 DAZAOG010000000
VERSION     DAZAOG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.284"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHDLD_003048"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHDLD_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHDLD_003049"
     CDS             394..477
                     /locus_tag="ACHDLD_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFN3364674.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHDLD_003050"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHDLD_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN3364675.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZAOG010000400         1350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.295, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000400 DAZAOG010000000
VERSION     DAZAOG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.295"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACHDLD_003051"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACHDLD_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACHDLD_003052"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACHDLD_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN3364676.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAZAOG010000401         1077 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.311, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000401 DAZAOG010000000
VERSION     DAZAOG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.311"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..881
                     /locus_tag="ACHDLD_003053"
     CDS             99..881
                     /locus_tag="ACHDLD_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFN3364677.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          377 a          147 c          216 g          337 t
ORIGIN      
        1 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
       61 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
      121 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
      181 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
      241 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
      301 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
      361 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
      421 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
      481 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
      541 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
      601 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
      661 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
      721 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
      781 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
      841 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
      901 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
      961 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
     1021 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtga
//
LOCUS       DAZAOG010000402          938 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.327, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000402 DAZAOG010000000
VERSION     DAZAOG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.327"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..867
                     /locus_tag="ACHDLD_003054"
                     /note="aph(2'')-Ia"
     CDS             <1..867
                     /locus_tag="ACHDLD_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFN3364678.1"
                     /translation="LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKK
                     GYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEE
                     EQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYI
                     ESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYL
                     LEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIEN
                     GRKEIYKRTYKD"
BASE COUNT          399 a           66 c          153 g          320 t
ORIGIN      
        1 ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat cggtagtggt
       61 tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa atttagtact
      121 aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa tacaaattta
      181 gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga attatctata
      241 ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc tactatgtca
      301 gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca aatgcacggt
      361 ttagattata cagatattag tgaatgtact attgataata aacaaaatgt attagaagag
      421 tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa agattatata
      481 gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc
      541 cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt aactggaata
      601 attgattttg gagattctgg aattatagat gaatattgtg attttatata cttacttgaa
      661 gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat gtatggaaat
      721 atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta tcctattgaa
      781 actattgttt atggaattaa aaatattaaa caggaattta tcgaaaatgg tagaaaagaa
      841 atttataaaa ggacttataa agattgatta tataatatat gaaaagctat tataaaagac
      901 ggttctgttg caaagtttta aatctactat caaataag
//
LOCUS       DAZAOG010000403          863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.341, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000403 DAZAOG010000000
VERSION     DAZAOG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.341"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..586
                     /gene="dfrG"
                     /locus_tag="ACHDLD_003055"
     CDS             89..586
                     /gene="dfrG"
                     /locus_tag="ACHDLD_003055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN3364679.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(658..>863)
                     /locus_tag="ACHDLD_003056"
     CDS             complement(658..>863)
                     /locus_tag="ACHDLD_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN3364680.1"
                     /translation="TTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKV
                     LTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          312 a          105 c          177 g          269 t
ORIGIN      
        1 ttcaactaac gggcaggata atgagaaaag gttttttgat agaaataaga aattagatgg
       61 taaattagtt aaaaaggctg tgttataaat gaaagtttct ttgattgctg cgatggataa
      121 gaatagagtg attggcaaag agaatgacat tccttggagg attcccaagg actgggaata
      181 tgttaaaaat actacaaagg gacatccgat aatattaggt aggaagaacc ttgaatcaat
      241 cggaagagcc ttacctgaca gaagaaatat tattctgacg agagataagg ggtttacctt
      301 taatggttgt gaaattgttc attcaataga agatgttttt gagttatgta aaaacgaaga
      361 agaaattttt attttcggag gagaacagat ttataatttg tttttccctt atgttgagaa
      421 aatgtacatc acaaaaatac atcatgaatt cgaaggagat actttttttc cagaagtgaa
      481 ttatgaggaa tggaatgagg tatttgccca aaaagggata aagaatgata aaaatccgta
      541 taactactat tttcatgtat atgaaagaaa aaacttattg agttaacggg tgcgttaatt
      601 gaagaagaac agtaaagata aagcacaaga gcttgggatt actccaagct ctttttgtca
      661 tttgaaataa attatatctt tccaatcaaa ttgtttatct gtaatcccta atctttgagc
      721 aggtgtcaaa acctttttat ccgctgattt aaaagggcga caaaagttgt agtaagttct
      781 tagaattgta atcgcaaatt gagcatactt cggattgaag ttagcgtaaa tatagctctt
      841 cttatcacca cgagctgttg tta
//
LOCUS       DAZAOG010000404          482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5128
            SAMEA115707514-rid22639393.guided.379, whole genome shotgun
            sequence.
ACCESSION   DAZAOG010000404 DAZAOG010000000
VERSION     DAZAOG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707514
            Sequence Read Archive: ERR13197243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002296210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002296210.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/19/2024 01:22:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 44 of 87
            Pseudo Genes (internal stop)      :: 25 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707514-rid22639393.guided.379"
                     /isolate="VRE5128"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>482
                     /locus_tag="ACHDLD_003057"
     CDS             <1..>482
                     /locus_tag="ACHDLD_003057"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3364681.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     "
BASE COUNT          198 a           48 c           86 g          150 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 ta
//
